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Immune suppression and disturbances of normal leukocyte populations are side effects attributed to many antimalarial drugs and were concerns during a recent year-long placebo-controlled trial that compared daily primaquine (0.5 mg of base per kg of body weight per day) with weekly chloroquine (300 mg of base one time per week) for malaria prophylaxis. The study took place in Irian Jaya, Indonesia, from July 1994 to August 1995 and enrolled 129 Javanese men with normal glucose-6-phosphate dehydrogenase function. Tests for lymphocyte function and subset composition were conducted blindly on a cross-section of subjects during weeks 10 (n = 42) and 48 (n = 72) of supervised prophylaxis. Lymphocyte function, measured as the proliferative response of peripheral blood mononuclear cells to a panel of mitogens (pokeweed mitogen, phytohemagglutinin, and concanavalin A) and antigens (purified protein derivative of Mycobacterium tuberculosis and Clostridium tetani toxoid) and expressed as a stimulation index, allowed for statistical comparison between groups and sampling times. The lymphocyte subset composition for each group and time point was based on flow cytometry profiling, and the results were expressed as the mean percentages of CD3 (total T cells), CD19 (total B cells), CD4+ (T-helper and inducer cells), and CD8+ (T suppressor and cytotoxic cells), CD3/CD16+ CD56 (natural killer cells), CD3/anti-HLA-DR (activated T cells) cells and the CD4+/CD8+ ratios. Lymphocyte stimulation indices were statistically comparable among the placebo, primaquine, and chloroquine groups at both time points, although the primaquine group was distinguished by having repeatedly greater proportions of subjects with high ( > 3.0) stimulation indices. The lymphocyte subset profiles of these groups at both time points were also similar and undistorted relative to those of healthy reference populations matched for age, sex, and ethnicity. The results provide quantitative support for the safety of daily primaquine prophylaxis.

Chloroquine remains the first-line therapy for uncomplicated malaria in Indonesia. Among a series of trials of chloroquine for malaria on this archipelago conducted since 1990, we now report the highest risk of therapeutic failure yet observed. A clinical trial of standard chloroquine therapy for uncomplicated malaria at Arso PIR V in northeastern Indonesian Papua was conducted during 1995. We enrolled 104 non-immune subjects infected with Plasmodium falciparum (n = 55), P. vivax (n = 29), or P. falciparum plus P. vivax (n = 20) and administered supervised standard chloroquine therapy (10 + 10 + 5 mg/kg at 24-hour intervals). The 28-day cumulative incidence of therapeutic failure was 95% for P. falciparum, 84% for P. vivax, and 100% for mixed infections. Only one subject each for P. falciparum and P. vivax remained free of parasites at day 28. All recurrent parasitemias occurred with whole blood levels of chloroquine plus desethylchloroquine exceeding 100 ng/ml. These findings document almost complete failure of chloroquine against P. falciparum or P. vivax near the northeastern coast of Indonesian Papua.

Penggunaan deterjen dalam kegiatan rumah tangga dan industri dapat meningkatkan pencemaran lingkungan. Zat aktif yang umum digunakan dalam deterjen adalah Alkil Benzena Sulfonat Linear (LAS). Tujuan penelitian ini adalah untuk mengkaji kemampuan Pseudomonas aeruginosa dalam mendegradasi LAS yang terdapat pada tiga jenis detergen yang berbeda. Penelitian ini menggunakan Pseudomonas aeruginosa sebagai dekomposer dan menambahkan (NH4)2SO4, KH2PO4, K2HPO4, dan C6H12O6 sebagai sumber N, P, dan C. Sebagai sampel digunakan tiga jenis deterjen komersial (A, B, dan C) dengan variasi konsentrasi uji 35mg/L, 50mg/L, dan 100mg/L. Penurunan kadar LAS dalam detergen selama 30 hari proses biodegradasi ditetapkan secara Methylene Blue Active Substances (MBAS) dan menggunakan instrumen spektrofotometer Uv-Vis pada λ652 nm. Hasil penelitian menunjukkan pada hari ke 30 proses biodegradasi deterjen A, B, dan C dengan konsentrasi uji 35mg/L menghasilkan residu LAS berturut-turut sebesar 3,62; 4,66 dan 11,62 mg/L. Pada konsentrasi uji 50 mg/L, residu LAS pada deterjen A, B, dan C berturut-turut sebesar 7,71; 11,88; dan 21,16mg/L. Sedangkan pada konsentrasi uji 100mg/L, residu LAS pada deterjen A, B, dan C berturut-turut sebesar 27,98; 37,54; 66,81mg/L

Optimization of Drying Temperature Using Oven on Quality of Black Pepper and White Pepper Powder Pepper (Piper Nigrum L) is an Indonesian spice plant widely used for export and import activity. The post-harvest process of pepper affected the quality, especially on the drying process. One of secondary metabolite compound is piperin. Piperin is an active substance of alkaloid group giving a distinctive spicy flavor to the pepper. Drying process by using the oven could improve the quality of pepper. The results showed that the optimum temperature for white pepper was 85° C for 20 minutes with the water content of 10.65%, total mold/yeast was 1 x 104 colony/g, and piperin content of 4.58%. For Black pepper, the optimum temperature was at 105° C for 20 minutes with the moisture content of 7.84%, total mold/yeast was 1.3 x 104 colony/g and piperin 5,01%.Keywords: White Pepper, Black Pepper, Piperin Content.ABSTRAK Lada (Piper Nigrum L) adalah tanaman rempah Indonesia yang banyak digunakan untuk ekspor dan impor. Proses pasca panen lada yang tidak maksimal menyebabkan kualitas menurun. Lada memiliki senyawa metabolit sekunder berupa piperin. Piperin adalah zat aktif golongan alkoloid yang memberikan cita rasa pedas khas pada lada. Pengeringan dengan menggunakan oven mampu meningkatkan mutu lada. Hasil penelitian menunjukan suhu optimum untuk pengovenan lada putih adalah 85°C selama 20 menit dengan kadar air sebesar 10,65 %, AKK 1 x 104 koloni/ g, dan kadar piperin 4,58 %. Lada hitam efektif pada suhu optimum 105°C selama 20 menit dengan kadar air sebesar 7,84 %, AKK 1,3 x 104 koloni / g dan kadar piperin 5,01 %.Kata kunci : Lada Putih, Lada Hitam, Kadar Piperin

Acid Hydrolysis on The Starch Flour of white sweet potato (Ipomoea batatas L.) in Making of Liquid Sugar National sugar needs for both direct consumption and for industrial needs will continue to increase as the population increases. According to Dirjenbun, in 2014 the national sugar demand reaches 5.7 million tons. Consisting of 2.8 million tons of white crystalline sugar for direct community consumption and 2.9 million tons of refined crystal sugar to meet industrial needs. White sweet potato can be used as raw material for making liquid glucose through hydrolysis process with acid (HCl). The preparation of liquid glucose consists of two stages: gelatinization stage and hydrolysis stage. Optimum hydrolysis was determined by variations in HCl concentration of 0.25; 0.5; and 0.75 N and time variations of 30, 60, and 90 minutes. The yield of sweet potato starch was 28.82% and the highest yield of hydrolysis result of white sweet potato starch was 94.07% at acid concentration 0.75 N with hydrolysis time 90 minutes. Keywords: white sweet potato, Flour of white sweet potato, liquid sugar, acid hydrolysis ABSTRAK Kebutuhan gula nasional baik untuk konsumsi langsung maupun untuk kebutuhan industri akan terus meningkat sejalan dengan meningkatnya jumlah penduduk. Menurut Dirjenbun, pada tahun 2014 kebutuhan gula nasional mencapai 5,7 juta ton yang terdiri dari 2,8 juta ton gula kristal putih untuk konsumsi masyarakat langsung dan 2,9 juta ton gula kristal rafinasi untuk memenuhi kebutuhan industri. Ubi jalar putih dapat dijadikan bahan baku pembuatan glukosa cair melalui proses hidrolisis dengan asam (HCl). Pembuatan glukosa cair terdiri dari dua tahap yaitu tahap gelatinisasi dan tahap hidrolisis. Hidrolisis optimum ditentukan dengan variasi konsentrasi HCl yaitu 0,25; 0,5; dan 0,75 N dan variasi waktu 30, 60, dan 90 menit. Rendemen pati ubi jalar didapatkan sebesar 28,82% dan rendemen glukosa tertinggi hasil hidrolisis pati ubi jalar putih sebesar 94,07% pada konsentrasi asam 0,75 N dengan waktu hidrolisis 90 menit. Kata kunci: Ubi jalar putih, Tepung Pati Ubi Jalar Putih, Gula cair, hidrolisis asam

Washing Effects of Chloride Content in Process of Making Carrageenan of Seaweed, Eucheuma cottonii This study was conducted experimentally using Completely Randomized Design (CRD) with three (3) of standard treatments and three (3) replications. Stages of treatments included: Extraction of seaweed with three stages: extraction of seaweed with normal washing process, the twice extraction of the seaweed with laundering at the beginning, and four times extraction of the seaweed with the washing at the end. Drying of seaweed extraction, milling of dried seaweed to be come carrageenan, and filtering. Carrageenan screening results conducted to determine the yield, test physical parameters such as viscosity, pH, water content, the gel strength, and ash content. And also test the chemical parameters such as levels of sulphate and chloride levels (as cleaning KCl based). The results showed that the washing process four times in the end give chloride levels (as KCl based cleaning) was lowest with an average of 0,16%, compared to the treatment washing process twice in the beginning with an average of 0,33%. And the manual process of KCl based cleaning with an average of 0,49% therefore the normal process of providing value levels of chloride as KCl based cleaning was the highest. The results of the study concluded that the washing process at the beginning and at the end, affect the chloride levels based cleaning obtained at below 2%. The washing process that can be used for the manufacture of carrageenan of the seaweed (Eucheuma cottonii) were the best in the washing process four times at the end. Keywords: Eucheuma cottonii, leaching, chloride, carrageenan ABSTRAK Penelitian ini dilaksanakan secara eksperimen dengan menggunakan metode Rancangan Acak Lengkap (RAL) dengan 3 (tiga) taraf perlakuan masing-masing 3 (tiga) kali ulangan. Tahapan kerja meliputi: Ekstraksi rumput laut dengan tiga tahapan yaitu ekstraksi rumput laut dengan proses pencucian normal, ekstraksi rumput laut dengan pencucian di awal sebanyak dua kali, dan ekstraksi rumput laut dengan pencucian di akhir sebanyak empat kali. Pengeringan hasil ekstraksi rumput laut, penggilingan rumput laut kering hingga menjadi karaginan, dan penyaringan . Hasil penyaringan karaginan dilakukan penentuan rendemen, uji parameter fisik seperti viskositas, pH, kadar air, kekuatan gel , dan kadar abu. Serta uji parameter kimia seperti kadar sulfat dan kadar klorida sebagai KCl dry based . Hasil yang diperoleh menunjukkan bahwa perlakuan proses pencucian empat kali di akhir memberikan nilai kadar klorida sebagai KCl dry based terendah dengan rata-rata 0,16%, dibandingkan dengan perlakuan proses pencucian dua kali di awal dengan rata-rata 0,33%, dan proses manual (sebagai KCl dry based ) dengan nilai rata-rata 0,49%, jadi proses normal memberikan nilai kadar klorida (sebagai KCl dry based ) yang menghasilkan nilai tertinggi. Hasil penelitian disimpulkan bahwa proses pencucian di awal dan di akhir, berpengaruh terhadap kadar klorida dry based yang diperoleh yaitu di bawah 2 %. Proses pencucian yang dapat digunakan untuk pembuatan karaginan dari rumput laut Eucheuma cottonii yang terbaik yaitu proses pencucian empat kali di akhir. Kata kunci : Euche uma cottonii , pencucian, klorida, karaginan

Study of Dynamics of Phosphate in the Water Quality in Bogor of Upstream Ciliwung RiverCiliwung River has existed and become an important part of the community since ancient period. Since 2009, the Ciliwung River has been polluted condition from upstream. One of the pollutant that could decrease the quality of river water was phosphate. Excessive phosphate level in water bodies caused nutrient enrichment conditions (eutrophication). The presence of nitrate supporting phosphate also caused algae blooming, one of the environmental problem. The research was conducted to determine the dynamics of the phosphate compound of Ciliwung River whether the pollution was reduced, same, or worse. The research included sampling of river water at three points of Katulampa, Pasar Bogor, and Warung Jambu River with sampling time interval of 8 hours in a day for 3 weeks in a row. The data of phosphate and nitrate concentration were measured and compared to the results in PP No.82 year 2001. The total of phosphate in Ciliwung river water has exceeded threshold level in accordance with the environmental quality standard of PP. 82 year 2001, and has been indicated to be in eutrophication condition.Keywords: Ciliwung River, Water Quality, Water Pollution, Phosphate.ABSTRAKSungai Ciliwung telah ada dan menjadi bagian penting masyarakat sejak zaman purba. Namun seiring dengan berlalunya waktu dan perkembangan pesat, sejak tahun 2009 Sungai Ciliwung telah tercemar dari hulu. Salah satu polutan yang bisa menurunkan kualitas air sungai adalah fosfat. Keberadaan fosfat yang berlebihan di badan air dapat menyebabkan kondisi pengayaan nutrisi (eutrofikasi), dan dengan dukungan nitrat dapat menyebabkan algae blooming yang menjadi salah satu masalah lingkungan. Penelitian ini dilakukan untuk mengetahui dinamika senyawa fosfat Sungai Ciliwung apakah pencemarannya berkurang, sama, atau lebih buruk. Ruang lingkup penelitian meliputi pengambilan sampel air sungai pada tiga titik Katulampa, Pasar Bogor, dan Warung Jambu dengan interval waktu sampling 8 jam dalam sehari, dan dilakukan setiap minggu selama 3 minggu berturut-turut. Kemudian dilakukan analisis sampel di laboratorium, serta interpretasi data dengan membandingkan hasilnya terhadap PP No.82 tahun 2001. Nilai total fosfat di air sungai Ciliwung Hulu tidak memenuhi standar kualitas lingkungan PP. 82 tahun 2001, dan diindikasikan berada dalam kondisi eutrofikasi.Kata kunci: Sungai Ciliwung, Kualitas Air, Polusi Air, Fosfat.

Phytochemical Content and Katinon Coumpound in Red Khat (Catha edulis) Leaves Khat Plant (Catha edulis) belongs to the Celastraceae family that originated in East Africa and the Arabian plateau. Khat plant has green leaves with finely toothed oval shape resembling betel leaves and fragrant. The active component Khat leaves was cathinone which is an alkaloids group. Cathinone which has the chemical formula of 2-amino-1-phenyl propanone is often referred to as a natural amphetamine because it produced effects like amphetamine that could penetrate the nervous system , adrenaline and as stimulant. The purpose of this study was to identify phytochemical compounds in red khat leaves (Catha edulis) using gas chromatography-mass spectra. The results showed that extracts of red leaf khat (Catha edulis) contained of secondary metabolites, they were alkaloids, phenolics, glycosides, steroids / triterpenoids, flavonoids, tannins and saponins. Based on the identification of khat leaf extract (Catha edulis) at pH 8, 9 and 10 showed the presence of katin compounds at different concentrations, while the cathinone compounds were not identified at all three types of the extract. Keywords : Catha edulis, phytochemical ,metabolite secondary, gas chromatography-mass spectra ABSTRAK Tanaman Khat ( Catha edulis ) termasuk dalam famili Celastraceae yang berasal dari Afrika Timur dan dataran Arab. Tanaman Khat memiliki daun berwarna hijau bergerigi halus dengan bentuk oval menyerupai daun sirih, dan berbau harum. Komponen aktif daun Khat yaitu katinona merupakan senyawa kimia golongan alkaloid. Katinona dengan rumus kimia 2-amino-1-fenil propanon sering disebut sebagai amfetamin alami karena menghasilkan efek seperti amfetamin yang bisa menembus susunan saraf pusat, memacu adrenalin dan stimulansia. Tujuan penelitian ini untuk mengidentifikasi kandungan senyawa fitokimia dan katinon dalam daun khat merah ( Catha edulis ) menggunakan kromatografi gas spektra massa .Hasil penelitian menunjukan serbuk simplisia daun khat merah ( Catha edulis ) mengandung senyawa metabolit sekunder yaitu alkaloid, fenolik, glikosida, steroid/triterpenoid, flavonoid, tanin serta saponin. Berdasarkan identifikasi ekstrak daun khat merah ( Catha edulis ) pada pH 8, 9 dan 10 menggunakan KG-SM menunjukan adanya senyawa katin pada konsentrasi yang berbeda, sedangkan senyawa katinona tidak ditemukan pada ke tiga jenis pH ekstrak tersebut. Kata kunci : Catha edulis, fitokimia , metabolit sekunder , kromatografi gas-spektra massa

The activity of ligninolytic enzymes of white rot fungi (WRF) Omphalina sp. and Pleurotus ostreatus was observed during somatic and fruiting body development in solid substrate fermentation using empty fruit bunches of oil palm (EFB). The enzyme activity was dominated by laccase both of Omphalina sp. and P. ostreatus. The laccase activity on somatic phase (mycelium growth) was higher compared to that of fruiting body formation phase. The laccase activity of Omphalina sp. was slightly higher compared to that of P. ostreatus. The peak activity of manganese peroxidase (MnP) for Omphalina sp. was observed two weeks after inoculation, while P. ostreatus had two peaks i.e. two and four weeks after inoculation period. The MnP activity of P. ostreatus was higher compared to that of Omphalina sp. Omphalina sp. growth in EFB did not secrete lignin peroxidase (LiP) in contrast to P. ostreatus. The peak of LiP activity of P. ostreatus was reached two and four weeks after inoculation. The MnP and LiP activities declined during the development of fruiting bodies while laccase increased both in Omphalina sp. and P. ostreatus. It seems that the activities of ligninolytic enzyme profile were regulated in line with developmental phase of growth both in Omphalina sp. and P. ostreatus. Key words: ligninolytic enzyme activity, Omphalina sp., Pleurotus ostreatus, empty fruit bunches of oil palm

Greenhouse gas (GHG) emissions from post-consumer waste and wastewater are a small contributor (about 3%) to total global anthropogenic GHG emissions. Emissions for 2004-2005 totalled 1.4 Gt CO2-eq year—1 relative to total emissions from all sectors of 49 Gt CO2-eq year— 1 [including carbon dioxide (CO2), methane (CH4), nitrous oxide (N2O), and F-gases normalized according to their 100-year global warming potentials (GWP)]. The CH4 from landfills and wastewater collectively accounted for about 90% of waste sector emissions, or about 18% of global anthropogenic methane emissions (which were about 14% of the global total in 2004). Wastewater N2O and CO2 from the incineration of waste containing fossil carbon (plastics; synthetic textiles) are minor sources. Due to the wide range of mature technologies that can mitigate GHG emissions from waste and provide public health, environmental protection, and sustainable development co-benefits, existing waste management practices can provide effective mitigation of GHG emissions from this sector. Current mitigation technologies include landfill gas recovery, improved landfill practices, and engineered wastewater management. In addition, significant GHG generation is avoided through controlled composting, state-of-the-art incineration, and expanded sanitation coverage. Reduced waste generation and the exploitation of energy from waste (landfill gas, incineration, anaerobic digester biogas) produce an indirect reduction of GHG emissions through the conservation of raw materials, improved energy and resource efficiency, and fossil fuel avoidance. Flexible strategies and financial incentives can expand waste management options to achieve GHG mitigation goals; local technology decisions are influenced by a variety of factors such as waste quantity and characteristics, cost and financing issues, infrastructure requirements including available land area, collection and transport considerations, and regulatory constraints. Existing studies on mitigation potentials and costs for the waste sector tend to focus on landfill CH4 as the baseline. The commercial recovery of landfill CH4 as a source of renewable energy has been practised at full scale since 1975 and currently exceeds 105 Mt CO2 -eq year—1. Although landfill CH 4 emissions from developed countries have been largely stabilized, emissions from developing countries are increasing as more controlled (anaerobic) landfilling practices are implemented; these emissions could be reduced by accelerating the introduction of engineered gas recovery, increasing rates of waste minimization and recycling, and implementing alternative waste management strategies provided they are affordable, effective, and sustainable. Aided by Kyoto mechanisms such as the Clean Development Mechanism (CDM) and Joint Implementation (JI), the total global economic mitigation potential for reducing waste sector emissions in 2030 is estimated to be > 1000 Mt CO2-eq (or 70% of estimated emissions) at costs below 100 US$ t— 1 CO2-eq year—1. An estimated 20—30% of projected emissions for 2030 can be reduced at negative cost and 30—50% at costs < 20 US$ t—1 CO 2-eq year—1. As landfills produce CH 4 for several decades, incineration and composting are complementary mitigation measures to landfill gas recovery in the short- to medium-term — at the present time, there are > 130 Mt waste year— 1 incinerated at more than 600 plants. Current uncertainties with respect to emissions and mitigation potentials could be reduced by more consistent national definitions, coordinated international data collection, standardized data analysis, field validation of models, and consistent application of life-cycle assessment tools inclusive of fossil fuel offsets.

The absolute necessity for rational therapy in the face of rampant drug resistance places increasing importance on the accuracy of malaria diagnosis. Giemsa microscopy and rapid diagnostic tests (RDTs) represent the two diagnostics most likely to have the largest impact on malaria control today. These two methods, each with characteristic strengths and limitations, together represent the best hope for accurate diagnosis as a key component of successful malaria control. This review addresses the quality issues with current malaria diagnostics and presents data from recent rapid diagnostic test trials. Reduction of malaria morbidity and drug resistance intensity plus the associated economic loss of these two factors require urgent scaling up of the quality of parasite-based diagnostic methods. An investment in anti-malarial drug development or malaria vaccine development should be accompanied by a parallel commitment to improve diagnostic tools and their availability to people living in malarious areas.

Insecticide toxicity of profenofos and chlorpyrifos on nile tilapia (Oreochromis sp .) The purpose of this study was to determine the level of toxicity of two organophosphate pesticide chlorpyrifos and profenofos on the nile tilapia ( Oreochromis . Sp). Applications for pest control of insect pests on plants may indirectly increase production, but some may find the negative impacts. Although a kind of pesticide intended to kill a specific target group or species, but is intrinsically toxic to all organisms for ecosystem sustainability. Profenofos and chlorpyrifos is a broad-spectrum organophosphate insecticide used to control insect pests on pepper, cotton, corn, onions, potatoes, vegetables and other crops. A number of the available data indicate that profenofos and chlorpyrifos are also harmful to non-target organisms in terrestrial and aquatic ecosystems. Scope o the study include Aclimitation Test, Preliminary and Definitive Determination and Test Range. The results showed the value of LC 50 was getting smaller with increasing observation time in both types of insecticides, results for profenofos LC50-24 hours 0.866 mg / L, LC50-48 hours 0.786 mg / L, LC50-72 hours 0,711 mg / L and 0,517 hours LC50-96 mg / L and for chlorpyrifos was LC50-24 hour 0.227 mg / L, LC50-48 hours of 0,211 mg / L, LC50-72 hours 0.192 mg / L and LC50-96 hours 0.173 mg / L, these results indicated that the insecticide profenofos and chlorpyrifos were insecticides with extremely high toxicity. LC50 values of insecticide chlorpyrifos was 3.6 times lower than the toxicity of profenofos. Keywords: profenofos , chlorpyrifos , LC 50, Oreochromis. sp ABSTRAK Tujuan dari penelitian ini adalah untuk mengetahui tingkat toksisitas dari dua pestisida organofosfat yaitu profenofos dan Klorpirifos terhadap ikan nila ( O reochromis . sp ). Aplikasi untuk penanggulangan hama serangga pengganggu pada tanaman secara tidak langsung dapat meningkatkan produksi, namun tidak sedikit pula ditemukan dampak negatifnya. Meskipun suatu jenis pestisida ditujukan untuk mematikan suatu kelompok atau spesies target tertentu, tetapi pada hakekatnya bersifat racun terhadap semua organisme untuk keberlanjutan ekosistem. Profenofos dan Klorpirifos merupakan insektisida organofosfat berspektrum luas yang digunakan untuk mengendalikan hama serangga pada cabai, kapas, jagung, bawang, kentang, sayur-sayuran dan tanaman lainnya. Sejumlah data yang tersedia menunjukkan bahwa profenofos dan klorpirifos juga berbahaya untuk organisme non-target pada ekosistem terestrial dan perairan.Ruang lipngkup penelitian ini meliputi Aklimitisasi, Uji Pendahuluan dan Penentuan Kisaran dan Uji Definitif. Hasil penelitian menunjukan nilai LC 50 semakin kecil dengan bertambahnya waktu pengamatan pada kedua jenis insektisida, hasil LC50-24 jam 0,866 mg/L, LC50-48 jam 0,786 mg/L, LC50-72 jam 0,711 mg/L dan LC50-96 jam 0,517 mg/L dan untuk klorpirifos didapatka hasil LC50-24 jam 0,227 mg/L, LC50-48 jam 0,211 mg/L, LC50-72 jam 0,192 mg/L dan LC50-96 jam 0,173 mg/L, hasil ini menunjukan bahwa insektisida profenofos dan klorpirifos adalah jenis insektisida dengan daya racun yang sangat tinggi. Nilai LC50 insektisida klorpirifos rata-rata pada tiap waktu pajanan 3,6 kali lebih rendah dibandingkan dengan profenofos sehingga daya racun klorpirifos lebih kuat dibandingkan dengan profenofos. Kata kunci : Profenofos, Klorpirifos, LC 50, Oreochromis. sp

Chloride Acid Hydrolysis of Cassava (Manihot esculenta Crantz) Strach Flour in Producing a Liquid Sugar Cassava (Manihot esculenta Crantz), known as cassava or manioc is a tuber or root of a tree that long with a diameter average of 5-10 cm and a length of 50-80 cm, depending on the type of cassava. Cassava is agricultural products that having high potency source of carbohydrates for food and industrial materials. Cassava starch can be made for liquid sugar by the method of acid hydrolysis using hydrochloric acid. The research results showed that rendemen at a HCl concentration of 0.75 N and hydrolysis time of 90 minutes was 80.51%. The higest rendemen of liquid sugar at a concentration of 0.5 N HCl and the hydrolysis time of 90 minutes was 84.22%. Results of the analysis indicate liquid sugar content of 16.22% moisture, ash content of 1.46%, 2.16% protein content, fat content of 0.53%, carbohydrates of 63.90%, and a negative starch content.Key words: Cassava, Flour Cassava Starch, Acid Hydrolysis, Liquid Sugar ABSTRAK Singkong (Manihot esculenta Crantz) yang dikenal sebagai ketela pohon atau ubi kayu merupakan umbi atau akar pohon yang rata-rata berdiameter 5-10 cm dan panjang 50-80 cm, tergantung dari jenis singkong. Singkong merupakan hasil produk pertanian yang potensinya tinggi sebagai sumber karbohidrat untuk bahan pangan dan industri. Pati singkong dapat dibuat gula cair dengan metode hidrolisis asam menggunakan asam klorida. Hasil penelitian menunjukkan bahwa nilai rendemen tertinggi pada konsentrasi HCl 0,75 N dan waktu hidrolisis 90 menit yaitu sebesar 80,51%. Rendemen tertinggi kadar gula pereduksi dari pembuatan gula cair dengan konsentrasi HCl 0,5 N dan waktu hidrolisis 90 menit sebesar 84,22%. Hasil analisa gula cair menunjukkan kandungan kadar air 16,22%, kadar abu 1,46%, kadar protein 2,16%, kadar lemak 0,53% , karbohidrat 63,90%, dan kandungan pati negatif.Kata kunci: Singkong, Tepung Pati Singkong, Hidrolisis Asam, Gula Cair

Optimation of Polyethylene Glycol (PEG) 6000 Treatment on Isolation of Agarose Seaweeds of Glacilaria sp Isolation of agarose from seaweeds (Glacilaria sp ) ha d been done in acid, base, and neutral condition by using polyethylene glycol (PEG) method. Glacilaria sp , Seaweeds that used in this research was cultured by f a rmer in Muara Gembong, Bekasi. It used PEG with concentration 10%, 15%, 20%, 25%, & 30%. The sc ope of this research ware determination of seaweeds, seaweeds water content, isolation of agarose from seaweeds, and quality test of agarose. Parameter test of agarose quality include the strength of gel, the content of sulfat, measurement of galactose content and its proksimat. The high yield was 61,27% r in PEG 30%. The best quality of agarose was resulted in PEG 20% because of the highest strength of gel was 379,04 g/cm 2 .The lowest sulfat concentration was 1,33% and galactose was 17,39%. Key words: polyethylene glycol, PEG 6000, agarose, seaweeds, glacilaria sp ABSTRAK Isolasi agarosa dari rumput laut Glacilaria sp telah dilakukan dalam suasana asam, basa dan netral dengan metode polyethylene glikol (PEG). Rumput lauat yang digunakan adalah Gracilaria sp yang dibudidayakan oleh petani di Muara Gembong, Bekasi dengan perlakuan konsentrasi PEG 10%, 15%, 20%, 25% dan 30%. Ruang lingkup penelitian ini meliputi uji determinasi rumput laut, penetapan kadar air rumput laut, isolasi agarosa dari rumput laut, serta uji kualitas agarosa. Parameter pengujian kualitas agarosa terdiri dari kekuatan gel, kandungan sulfat, dan pengukuran kandungan galaktosa serta proksimatnya. Isolasi agarosa perlakuan konsentrasi polyethylene Glikol (PEG) 6000 pada rumput laut Glacilaria sp diperoleh rendemen paling tinggi terdapat pada perlakuan PEG 30% yaitu sebesar 61,27%. Akan tetapi untuk kualitas agarosa yang paling baik terdapat pada perlakuan konsentrasi PEG 20% karena diperoleh kekuatan gel paling tinggi sebesar 379,04 g/cm 2 . Kadar sulfat yang paling rendah sebesar 1,33% dan kadar galaktosa sebesar 17,39%. Kata Kunci: polyethylene glikol, PEG 6000, agarosa, rumput laut, glacilaria sp

Proximate and Gel Strenght Analysis of Agar f rom Dry Seaweed in some Traditional Market s Seaweeds a re good material in processing to intermedite and finish product like agar – agar s in Indonesia still in a semi- modern and traditional methods . This led Indonesia still make import the agar gelatin from other countries. The purpose of the study was to increas use the dried seaweed in traditional markets f o r the products of gelatin and to provide the nutrition information, like water content, ash content, crude fat, protein, carbohydrates, crude fiber, the levels of sulfate and gel strength of processed gelatin from dry seaweed. Dried seaweed used in this study, obtained from a traditional market in Bogor, Bekasi and Jakarta. Proximate result and the gel strength from extracted agar powder samples would compared with commercial flour agar . Research results obtained extracted agar – agar powder samples had lower chemical content and gel strength than the commercial agar , but it still could be used as food, although in very small amounts. Keywords : dry seaweeds, proximate analysis, gel strength, agar – agar ABSTRAK Pengolahan rumput laut menjadi bahan olahan seperti agar – agar di Indonesia masih secara tradisional dan semi modern. Hal ini menyebabkan Indonesia masih mengimpor agar – agar dari negara lain. Manfaat dan tujuan penelitian ini adalah memanfaatkan rumput laut kering yang banyak terdapat di pasar – pasar tradisional menjadi produk agar – agar dan memberikan informasi kandungan nutrisi yang berupa air, abu, lemak kasar, protein, karbohidrat, serat kasar, kadar sulfat serta kekuatan gel dari agar –agar hasil olahan rumput laut kering. Rumput laut kering yang digunakan pada penelitian ini, diperoleh dari pasar tradisional di Bogor, Bekasi dan Jakarta. Hasil proksimat dan kekutan gel dari sampel tepung agar – agar hasil ekstrak kemudian dibandingkan dengan tepung agar – agar komersial berdasarkan hasil penelitian diperoleh sampel tepung agar – agar hasil ekstraksi dengan kandungan kimia dan kekuatan gel yang lebih rendah dari agar – agar komersial, tetapi masih dapat dimanfaatkan sebagai bahan pangan walaupun dalam jumlah yang sedikit. Kata kunci : rumput laut kering, analisis proksimat, kekuatan gel, agar – agar

Identification of Chemical Compounds at the Leuncas Fruits Base Extracts with Chromatography Methodes Secondary methabolites contain in the plant was a alkaloids, flavonoids, terphenoids, steroids that use for medicinal, biopestiside, etc. Taksonomy principle of Leunca (Solanum nigrum Linn) is clasified to Solanaceae family, and prediction contains alkaloids. The leunca fruits base extracts qualitative tested with Dragendorf reagents and indetified with thin layer chromatography (TLC), coloumn chromatography (CC), and gass chromatography mass-spectra (GC-MS). Optimation of eluens as mobile fase for TLC and CC used three eluens, there are methanol: NH 4 OH (200:3), chloroform:methanol (17:3), and chloroform:ethanol (4:1). Dragendorf test showed extract contains alkaloids compounds. Eluens optimum was chloroform:methanol (17:3) with trhree fraction ,and GC-MS identification showed that extract contains chemical compounds as carbocilic acid, thiourea, nicotinic acid, and glicerol. Keyword s: Alkaloids compounds, leunca, chromatography ABSTRAK Metabolit sekunder yang terkandung pada tanaman dapat berupa senyawaan kimia alkaloid, flavonoid, terpenoid, dan steroid . Senyawaan kimia ini dapat berfungsi sebagai obat, biopestisida, dan sebagainya. Leunca ( Solanum nigrum Linn) berdasarkan ilmu taksonomi diklasifikasikan ke dalam famili Solanaceae (labu-labuan), dan diduga banyak mengandung senyawaan alkaloid yang berkhasiat sebagai obat. Ekstrak basa buah lenca diuji dengan pereaksi Draggendorf dan diidentifikasi dengan menggunakan teknik kromatografi lapis tipis (KLT), kromatografi kolom, dan instrumen kromatografi gas-spektra massa (GC-MS). Optimasi eluen sebagai fase gerak pada KLT dan kromatografi kolom dilakukan terhadap 3 jenis dan komposisi eluen yang berbeda yaitu Metanol : NH 4 OH (200:3), Kloroform:Metanol (17:3), dan Kloroform:Etanol (4:1). Hasil uji Dragendorf mengindikasikan bahwa ekstrak basa buah lenca mengandung senyawaan alkaloid. Hasil optimasi eluen menunjukkan bahwa eluen yang optimum adalah Kloroform:Metanol (17:3) dengan memberikan 3 fraksi pemisahan. Nilai Rf ketiga fraksi tersebut adalah 0,21; 0,31 dan 0,60. Berdasarkan analisis dengan GC-MS diketahui bahwa ekstrak basa buah lenca mengandung senyawaan kimia karboksilat, tiourea, asam nikotinat, dan gliserol. Kata kunci : Senyawa alkaloid, leunca, kromatografi

Background Conducting Controlled Human Malaria Infection (CHMI) studies in sub-Saharan Africa presents unique challenges yet it provides enormous opportunities for fast-tracking malaria vaccine and drug development. Sanaria Inc. USA, has devised methods of producing, characterising and shipping aseptic, purified, cryopreserved Plasmodium falciparum sporozoites (PfSPZ). Unlike in the past, the sporozoites can be shipped to any field site including malaria-endemic countries in sub-Saharan Africa. CHMI studies need cutting-edge laboratory capacity to allow screening of potential study participants for their eligibility, perform safety tests, efficacy testing of the investigation product, and determine immune markers, responses and parasite kinetics. Methods The sites were identified within sub-Saharan Africa, based on local population, malaria epidemiology status and clinical research capacity. The laboratory assays, equipment and consumables were identified. The methods and procedures were optimised, standardised and documented. The laboratory technical team were selected and trained. EDCTP provided financial support whilst Sanaria Inc. provided technical support. Results The capacity strengthening successfully supported CHMI studies in five sites in Africa namely; IHI, Bagamoyo, Tanzania; KEMRI CRC, Nairobi, Kenya; EGMVI, Malabo Equatorial Guinea; CERMEL, Lambarane, Gabon; KEMRI-CDC, Siaya-Kenya and KEMRI-WT, Kilifi Kenya. Capacity strengthening was aimed at clinical and immunology laboratory systems required for CHMI studies. Conclusions As more CHMI studies are anticipated, vigorous capacity strengthening will be required for laboratory facilities in Africa to accelerate the evaluation of malaria vaccines, anti-malaria drugs, diagnostic assays and assessment of host immune response to malaria infection.

The OptiMAL assay, a new immunochromatographic "dipstick" test for malaria based on detection of Plasmodium lactate dehydrogenase (pLDH), is purported to detect infections of approximately 200 parasites/microL of blood and to differentiate between Plasmodium falciparum and non-P. falciparum. We evaluated OptiMAL performance by comparing the test strip interpretations of two independent readers with consensus results obtained independently by expert malaria microscopists. Unbiased measures of sensitivity were derived by applying the OptiMAL test for detection and differentiation of light, asymptomatic infections by P. falciparum and Plasmodium vivax. OptiMAL readings were separated in time to determine whether the reaction signal was stable. Microscopy identified infections in 225 of 505 individuals screened; those with P. falciparum (n = 170) averaged 354 asexual forms/microL and P. vivax/Plasmodium malariae (n = 112) averaged 216 asexual forms/microL of blood. Concordance between OptiMAL and microscopy was 81% and 78% by the two independent readings. The assay's sensitivity for detection of any malaria species was 60.4% and 70.2% respectively and specificity was 97% and 89%. Most cases identified by microscopy as P. falciparum were graded as negative or non-falciparum by both OptiMAL readers. OptiMAL false negatives as well as misidentifications were related to low parasitemias (< 500/microL). The OptiMAL assay demonstrated 88-92% sensitivity for detecting infections of 500-1,000 parasites/microL, a range covering the mean parasitemia of primary symptomatic P. falciparum infections in malaria-naive Indonesian transmigrants. This device was markedly less sensitive than expert microscopy for discriminating between malaria species and is presently unsuited for use as an epidemiological screening tool. The OptiMAL assay is not approved for diagnostic use but is commercially available for research purposes only.

Immune suppression resulting from prolonged chemoprophylaxis and potential drug-vaccine interaction were investigated within the context of a randomized placebo-controlled trial that compared daily primaquine or weekly chloroquine administration for malaria prevention. After 11 months of prophylaxis, adult male subjects received a tetanus-diphtheria (Td) vaccination. Prophylaxis continued 4 weeks longer. Anti-tetanus and anti-diphtheria antibody levels were measured by ELISA at baseline and at 1, 3, 7, and 14 months after Td vaccination. All groups were comparable at baseline. Immunization triggered significant increases in anti-tetanus and anti-diphtheria IgG levels over each group's pre-Td baseline levels and those of an unvaccinated control group. Geometric mean anti-tetanus titers (GMTs) in the primaquine group were significantly higher than those of the placebo group at 1, 3, and 14 months. Anti-tetanus GMTs in placebo and chloroquine groups declined over 14 months to levels comparable to those of unvaccinated controls, but levels in the primaquine group remained significantly higher than in controls.

In Indonesia, energy consumption (excluding non-commercial energy) increased from 328 MBOE in 1990 to 478 MBOE in 1995. As a consequence, energy sector CO2 emissions increased from 150 million tons to over 200 million tons during the same period. The present rapid economic growth Indonesia is experiencing (7–8%) will continue in the future. Based on a BAU scenario, primary energy supply for the year 2020 will be 18,551 PJ, an increase of 5.9% annually from 1990 CO2 from the energy system will increase from 150 Teragrams in 1990 to 1264 Teragram in 2020. The mitigation scenario would reduce total CO2 emissions from the BAU scenario by 10% for the year 2000 and 20% by 2020. Some demand side management and energy conservation programs are already included in the BAU scenario. In the mitigation scenario, these programs are expanded, leading to lower final energy demand in the industrial and residential sectors. Indonesia's total primary energy supply in 2020 is approximately 5% lower for the mitigation scenario than for the BAU scenario. In the BAU scenario, coal and oil have the same contribution (25%). In the mitigation scenario, natural gas and nonfossil fuels such as hydropower, geothermal, and nuclear have higher contributions.

Malaria Sporozoite Vaccine Each year, hundreds of millions of people are infected with Plasmodium falciparum , the mosquito-borne parasite that causes malaria. A preventative vaccine is greatly needed. Seder et al. (p. 1359 , published online 8 August; see the Perspective by Good ) now report the results from a phase I clinical trial where subjects were immunized intravenously with a whole, attenuated sporozoite vaccine. Three of 9 subjects who received four doses and zero of 6 subjects who received five doses of the vaccine went on to develop malaria after controlled malaria infection. Both antibody titers and cellular immune responses correlated positively with the dose of vaccine received, suggesting that both arms of the adaptive immune response may have participated in the observed protection.

Malaria Sporozoite Vaccine Each year, hundreds of millions of people are infected with Plasmodium falciparum, the mosquito-borne parasite that causes malaria. A preventative vaccine is greatly needed. Seder et al. (p. 1359, published online 8 August; see the Perspective by Good) now report the results from a phase I clinical trial where subjects were immunized intravenously with a whole, attenuated sporozoite vaccine. Three of 9 subjects who received four doses and zero of 6 subjects who received five doses of the vaccine went on to develop malaria after controlled malaria infection. Both antibody titers and cellular immune responses correlated positively with the dose of vaccine received, suggesting that both arms of the adaptive immune response may have participated in the observed protection. Consistent, high-level, vaccine-induced protection against human malaria has only been achieved by inoculation of Plasmodium falciparum (Pf) sporozoites (SPZ) by mosquito bites. We report that the PfSPZ Vaccine—composed of attenuated, aseptic, purified, cryopreserved PfSPZ—was safe and wel-tolerated when administered four to six times intravenously (IV) to 40 adults. Zero of six subjects receiving five doses and three of nine subjects receiving four doses of 1.35 × 105 PfSPZ Vaccine and five of six nonvaccinated controls developed malaria after controlled human malaria infection (P = 0.015 in the five-dose group and P = 0.028 for overall, both versus controls). PfSPZ-specific antibody and T cell responses were dose-dependent. These data indicate that there is a dose-dependent immunological threshold for establishing high-level protection against malaria that can be achieved with IV administration of a vaccine that is safe and meets regulatory standards. Intravenous immunization with an attenuated whole malaria sporozoite vaccine protected volunteers in a phase I clinical trial. [Also see Perspective by Good] Intravenous immunization with an attenuated whole malaria sporozoite vaccine protected volunteers in a phase I clinical trial. [Also see Perspective by Good]

Contains fulltext : 118242.pdf (Publisher’s version ) (Open Access) BACKGROUND: Gene-based vaccination using prime/boost regimens protects animals and humans against malaria, inducing cell-mediated responses that in animal models target liver stage malaria parasites. We tested a DNA prime/adenovirus boost malaria vaccine in a Phase 1 clinical trial with controlled human malaria infection. METHODOLOGY/PRINCIPAL FINDINGS: The vaccine regimen was three monthly doses of two DNA plasmids (DNA) followed four months later by a single boost with two non-replicating human serotype 5 adenovirus vectors (Ad). The constructs encoded genes expressing P. falciparum circumsporozoite protein (CSP) and apical membrane antigen-1 (AMA1). The regimen was safe and well-tolerated, with mostly mild adverse events that occurred at the site of injection. Only one AE (diarrhea), possibly related to immunization, was severe (Grade 3), preventing daily activities. Four weeks after the Ad boost, 15 study subjects were challenged with P. falciparum sporozoites by mosquito bite, and four (27%) were sterilely protected. Antibody responses by ELISA rose after Ad boost but were low (CSP geometric mean titer 210, range 44-817; AMA1 geometric mean micrograms/milliliter 11.9, range 1.5-102) and were not associated with protection. Ex vivo IFN-gamma ELISpot responses after Ad boost were modest (CSP geometric mean spot forming cells/million peripheral blood mononuclear cells 86, range 13-408; AMA1 348, range 88-1270) and were highest in three protected subjects. ELISpot responses to AMA1 were significantly associated with protection (p = 0.019). Flow cytometry identified predominant IFN-gamma mono-secreting CD8+ T cell responses in three protected subjects. No subjects with high pre-existing anti-Ad5 neutralizing antibodies were protected but the association was not statistically significant. SIGNIFICANCE: The DNA/Ad regimen provided the highest sterile immunity achieved against malaria following immunization with a gene-based subunit vaccine (27%). Protection was associated with cell-mediated immunity to AMA1, with CSP probably contributing. Substituting a low seroprevalence vector for Ad5 and supplementing CSP/AMA1 with additional antigens may improve protection. TRIAL REGISTRATION: ClinicalTrials.govNCT00870987.

Primakuin yang digunakan sebagai profilaksis malaria terbukti efektif dan diterima dengan baik oleh tubuh manusia yang normal terhadap aktivitas enzim 6 glukosa-6 fosfat dehidrogenase (G-6PD). Pemeriksaan laboratoris klinik adalah bagian dari uji coba secara acak dengan kontrol plasebo dalam rangka mengevaluasi penggunaan primakuin sebagai profilaksis pada penduduk transmigran yang tidak kebal di Irian Jaya. Penelitian ini dilakukan terhadap 129 pria Jawa dewasa yang normal G-6PDnya. Pemeriksaan hematologi, fungsi hati dan ginjal, dan pemeriksaan limfosit dilakukan berulang kali selama waktu penelitian profilaksis dilakukan untuk menjamin keamanan dari sukarelawan tersebut dan mengawasi perubahan yang mungkin terjadi akibat obat profilaksis. Seperti yang diperkirakan, pengguna primakuin tidak menunjukkan gejala peningkatan methemoglobin yang kembali dalam batas normal setelah 7 hari pemberian dosis terakhir. Pada akhir penelitian (12 bulan profilaksis) nilai hematologi, fungsi hati dan ginjal, dan nilai limfosit dari kelompok primakuin sebanding dengan kelompok plasebo, dan berada dalam batas nilai normal untuk orang Indonesia.Hasil penelitian ini memberikan masukan adanya keluhan fisik yang sedikit dari sukarelawan pengguna profilaksis primakuin. Untuk membuktikan hasil penelitian ini dan mempersiapkan penggunaan secara umum primakuin untuk profilaksis malaria, perlu dilakukan uji coba lebih lanjut keamanan primakuin. Di Indonesia, primakuin tidak digunakan sebagai profilaksis dan laporan hasil penelitian ini hendaknya tidak ditafsirkan sebagai laporan keamanan dari primakuin.

Background Accurate information about the burden of malaria infection at the district or provincial level is required both to plan and assess local malaria control efforts. Although many studies of malaria epidemiology, immunology, and drug resistance have been conducted at many sites in Indonesia, there is little published literature describing malaria prevalence at the district, provincial, or national level. Methods Two stage cluster sampling malaria prevalence surveys were conducted in the wet season and dry season across West Sumba, Nusa Tenggara Province, Indonesia. Results Eight thousand eight hundred seventy samples were collected from 45 sub-villages in the surveys. The overall prevalence of malaria infection in the West Sumba District was 6.83% (95% CI, 4.40, 9.26) in the wet season and 4.95% (95% CI, 3.01, 6.90) in the dry. In the wet season Plasmodium falciparum accounted for 70% of infections; in the dry season P. falciparum and Plasmodium vivax were present in equal proportion. Malaria prevalence varied substantially across the district; prevalences in individual sub-villages ranged from 0–34%. The greatest malaria prevalence was in children and teenagers; the geometric mean parasitaemia in infected individuals decreased with age. Malaria infection was clearly associated with decreased haemoglobin concentration in children under 10 years of age, but it is not clear whether this association is causal. Conclusion Malaria is hypoendemic to mesoendemic in West Sumba, Indonesia. The age distribution of parasitaemia suggests that transmission has been stable enough to induce some clinical immunity. These prevalence data will aid the design of future malaria control efforts and will serve as a baseline against which the results of current and future control efforts can be assessed.

Chloroquine remains the first-line therapy for uncomplicated malaria in Indonesia. Among a series of trials of chloroquine for malaria on this archipelago conducted since 1990, we now report the highest risk of therapeutic failure yet observed. A clinical trial of standard chloroquine therapy for uncomplicated malaria at Arso PIR V in northeastern Indonesian Papua was conducted during 1995. We enrolled 104 non-immune subjects infected with Plasmodium falciparum (n = 55), P. vivax (n = 29), or P. falciparum plus P. vivax (n = 20) and administered supervised standard chloroquine therapy (10 + 10 + 5 mg/kg at 24-hour intervals). The 28-day cumulative incidence of therapeutic failure was 95% for P. falciparum, 84% for P. vivax, and 100% for mixed infections. Only one subject each for P. falciparum and P. vivax remained free of parasites at day 28. All recurrent parasitemias occurred with whole blood levels of chloroquine plus desethylchloroquine exceeding 100 ng/ml. These findings document almost complete failure of chloroquine against P. falciparum or P. vivax near the northeastern coast of Indonesian Papua.

Chloroquine remains the first-line therapy for uncomplicated malaria in Indonesia. Among a series of trials of chloroquine for malaria on this archipelago conducted since 1990, we now report the highest risk of therapeutic failure yet observed. A clinical trial of standard chloroquine therapy for uncomplicated malaria at Arso PIR V in northeastern Indonesian Papua was conducted during 1995. We enrolled 104 non-immune subjects infected with Plasmodium falciparum (n = 55), P. vivax (n = 29), or P. falciparum plus P. vivax (n = 20) and administered supervised standard chloroquine therapy (10 + 10 + 5 mg/kg at 24-hour intervals). The 28-day cumulative incidence of therapeutic failure was 95% for P. falciparum, 84% for P. vivax, and 100% for mixed infections. Only one subject each for P. falciparum and P. vivax remained free of parasites at day 28. All recurrent parasitemias occurred with whole blood levels of chloroquine plus desethylchloroquine exceeding 100 ng/ml. These findings document almost complete failure of chloroquine against P. falciparum or P. vivax near the northeastern coast of Indonesian Papua.

Microscopy is an imperfect reference standard used for malaria diagnosis in clinical trials. The purpose of this study was to provide an assessment of the accuracy of basic microscopy, to compare polymerase chain reaction (PCR)-based diagnosis with microscopy results, and to assess the effect of microscopy error on apparent protective efficacy. The sensitivity and specificity of basic, compared with expert, microscopy was determined to be 91% and 71%, respectively. In a clinical trial, agreement between PCR and microscopy results improved with expert confirmation of initial results. In a simulated 12-week trial with weekly routine malaria smears, a very high specificity (>99%) for each malaria smear was found to be necessary for an estimate of protective efficacy to be within 10%-25% of the true value, but sensitivity had little effect on this estimate. Microscopy error occurs and can affect clinical trial results.

Synthesized T-cell epitopes of tetanus toxin are universally immunogenic and serve to enhance immune response when they are used as vaccine carriers of B-cell epitopes. The immunogenicity of the P2, P30, and P2P30 T-cell epitopes of tetanus toxin and whole tetanus toxoid (TT) was evaluated by in vitro proliferation assay of lymphocytes from men with no history of tetanus vaccination who were enrolled in a malaria prophylaxis trial. The enhancement of immune response by tetanus vaccination (Td) and possible antagonism by the antimalarial drugs, was measured by pre- and post-Td comparisons within and between immunized prophylaxis groups (primaquine, chloroquine, placebo) and a nonimmunized control group. Constructs demonstrated low immunogenicity relative to TT in all groups. Relative to both control and its own baseline, the immunized primaquine prophylaxis group was distinct in demonstrating significantly increased proliferation against all three subunits and at both high (30 microg ml(-1)) and low (3 microg ml(-1)) concentrations. Immunization elicited significantly increased proliferation responses by placebo and chloroquine prophylaxis groups against only the P2P30 construct. Despite these significant post-Td changes, a low concentration of TT 0.1 microg ml(-1)) stimulated proliferation 7-10 times over that induced by the greatest concentration of the constructs.

Immune suppression, a potential side effect of long-term chemoprophylaxis, was evaluated as part of a randomized, placebo-controlled trial that compared daily primaquine against weekly chloroquine for malaria prevention. In the last month of the year-long trial, baseline in vitro lymphoproliferative responses to tetanus toxoid were measured, and a tetanus-diphtheria (Td) immunization was administered. Proliferative responses to tetanus toxoid in each Td-immunized group increased significantly over pre-Td baselines and those of the unvaccinated control. Highest initial responses were measured in the primaquine group. The proportion of responders and the magnitude of proliferation was consistently low in the chloroquine group, and end point responses in this group were significantly below those of the placebo. These results suggest that the development and duration of the cellular response to tetanus immunization was impaired by long-term weekly chloroquine prophylaxis, while daily primaquine prophylaxis over the same time period had no inhibitory effect.

A malariometric survey was conducted in 14 villages of Sekotong district, in Lombok, Indonesia during October 1994. Point prevalence of malaria ranged from 0% to 15% in the surveyed villages, averaging 6% overall, and Plasmodium falciparum accounted for 63% of the infections. Forty-nine patients with uncomplicated malaria and parasite counts ranging from 40 to 10,800 asexual forms/µl were enrolled in a 28-day in vivo test of chloroquine sensitivity. All subjects received a supervised therapeutic regimen of chloroquine (25 mg base/kg over a 48-hr period) and parasitemia and symptoms were closely monitored for 28 days. Asexual parasites were eliminated within four days in the 29 P. falciparum and 20 P. vivax study patients enrolled. The cumulative incidence of therapeutic failure (recurrent symptomatic parasitemia) among P. falciparum cases at days 7, 14, and 28 was 7%, 10%, and 14% (4 of 29), respectively. However in all four cases, parasitemias recurred against chloroquine blood levels below the minimally effective concentration (MEC) of 200 ng/ml and do not confirm chloroquine resistance. All 20 P. vivax parasitemias were sensitive to chloroquine and the blood remained clear, with the exception of one case in which an asymptomatic parasitemia appeared on day 28. Parasitemias by P. falciparum and P. vivax that were observed before supervised therapy, but in the presence of whole blood chloroquine above normally suppressive MEC levels, suggest resistance to suppressive or prophylactic regimens of chloroquine.