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79 results on '"Sun, Qingtao"'

51. Generation of a whole-brain atlas for the cholinergic system and mesoscopic projectome analysis of basal forebrain cholinergic neurons

Author

Li, Xiangning, primary, Yu, Bin, additional, Sun, Qingtao, additional, Zhang, Yalun, additional, Ren, Miao, additional, Zhang, Xiaoyan, additional, Li, Anan, additional, Yuan, Jing, additional, Madisen, Linda, additional, Luo, Qingming, additional, Zeng, Hongkui, additional, Gong, Hui, additional, and Qiu, Zilong, additional

Published
2017
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63. High-throughput dual-colour precision imaging for brain-wide connectome with cytoarchitectonic landmarks at the cellular level

Author

Gong, Hui, primary, Xu, Dongli, additional, Yuan, Jing, additional, Li, Xiangning, additional, Guo, Congdi, additional, Peng, Jie, additional, Li, Yuxin, additional, Schwarz, Lindsay A., additional, Li, Anan, additional, Hu, Bihe, additional, Xiong, Benyi, additional, Sun, Qingtao, additional, Zhang, Yalun, additional, Liu, Jiepeng, additional, Zhong, Qiuyuan, additional, Xu, Tonghui, additional, Zeng, Shaoqun, additional, and Luo, Qingming, additional

Published
2016
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74. Simultaneous acquisition of neuronal morphology and cytoarchitecture in the same Golgi-stained brain

Author

Chen, Xiao, Zhang, Xiaoyu, Zhong, Qiuyuan, Sun, Qingtao, Peng, Jie, Gong, Hui, and Yuan, Jing

Abstract

Acquiring an accurate orientation reference is a prerequisite for precisely analysing the morphological features of Golgi-stained neurons in the whole brain. However, the same reflective imaging contrast of Golgi staining for morphology and Nissl staining for cytoarchitecture leads to the failure of distinguishing soma morphology and simultaneously co-locate cytoarchitecture. Here, we developed the dual-mode micro-optical sectioning tomography (dMOST) method to simultaneously image the reflective and fluorescent signals in three dimensions. We evaluated the feasibility of real-time fluorescent counterstaining on Golgi-stained brain tissue. With our system, we acquired whole-brain data sets of physiological and pathological Golgi-stained mouse model brains with fluorescence-labelled anatomical annotation at single-neuron resolution. We also obtained the neuronal morphology of macaque monkey brain tissue using this method. The results show that real-time acquisition of the co-located cytoarchitecture reference in the same brain greatly facilitates the precise morphological analysis of Golgi-stained neurons.

Published
2018

78. Dynorphin modulates motivation through a pallido-amygdala cholinergic circuit.

Author

Sun Q, Liu M, Guan W, Xiao X, Dong C, Bruchas MR, Zweifel LS, Li Y, Tian L, and Li B

Abstract

The endogenous opioid peptide dynorphin and its receptor κ-opioid receptor (KOR) have been implicated in divergent behaviors, but the underlying mechanisms remain elusive. Here we show that dynorphin released from nucleus accumbens dynorphinergic neurons exerts powerful modulation over a ventral pallidum (VP) disinhibitory circuit, thereby controlling cholinergic transmission to the amygdala and motivational drive in mice. On one hand, dynorphin acts postsynaptically via KORs on local GABAergic neurons in the VP to promote disinhibition of cholinergic neurons, which release acetylcholine into the amygdala to invigorate reward-seeking behaviors. On the other hand, dynorphin also acts presynaptically via KORs on dynorphinergic terminals to limit its own release. Such autoinhibition keeps cholinergic neurons from prolonged activation and release of acetylcholine, and prevents perseverant reward seeking. Our study reveals how dynorphin exquisitely modulate motivation through cholinergic system, and provides an explanation for why these neuromodulators are involved in motivational disorders, including depression and addiction.

Published
2024
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79. Area postrema neurons mediate interleukin-6 function in cancer-associated cachexia.

Author

Sun Q, van de Lisdonk D, Ferrer M, Gegenhuber B, Wu M, Tollkuhn J, Janowitz T, and Li B

Abstract

Interleukin-6 (IL-6) has been long considered a key player in cancer-associated cachexia 1-15 . It is believed that sustained elevation of IL-6 production during cancer progression causes brain dysfunctions, which ultimately result in cachexia 16-20 . However, how peripheral IL-6 influences the brain remains poorly understood. Here we show that neurons in the area postrema (AP), a circumventricular structure in the hindbrain, mediate the function of IL-6 in cancer-associated cachexia in mice. We found that circulating IL-6 can rapidly enter the AP and activate AP neurons. Peripheral tumor, known to increase circulating IL-6 1-5,15,18,21-23 , leads to elevated IL-6 and neuronal hyperactivity in the AP, and causes potentiated excitatory synaptic transmission onto AP neurons. Remarkably, neutralization of IL-6 in the brain of tumor-bearing mice with an IL-6 antibody prevents cachexia, reduces the hyperactivity in an AP network, and markedly prolongs lifespan. Furthermore, suppression of Il6ra , the gene encoding IL-6 receptor, specifically in AP neurons with CRISPR/dCas9 interference achieves similar effects. Silencing of Gfral-expressing AP neurons also ameliorates the cancer-associated cachectic phenotypes and AP network hyperactivity. Our study identifies a central mechanism underlying the function of peripheral IL-6, which may serve as a target for treating cancer-associated cachexia.

Published
2023
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