51. Expression of the protective antigen for PEDV in transgenic duckweed, Lemna minor
- Author
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Myung Jin Oh, Min-Jae Kim, Pyung Ok Lim, Hyun-Sook Sin, Hyeon-Jin Sun, Suk-Min Ko, Yong-Woo Kim, Hyo-Yeon Lee, Suk Weon Kim, In-Ja Song, and Chang-Hyo Goh
- Subjects
Lemna minor ,Lemna ,biology ,medicine.diagnostic_test ,Transgene ,Plant Science ,Horticulture ,biology.organism_classification ,Virology ,Diarrhea ,Western blot ,Infectious disease (medical specialty) ,medicine ,medicine.symptom ,Porcine epidemic diarrhea virus ,Gene ,Biotechnology - Abstract
Duckweeds are small, floating aquatic plants with a number of useful characteristics, including edibility, fast-growing, and a clonal proliferation. Duckweed is also fed to animals as a diet complement because of its high nutritional value. Porcine epidemic diarrhea virus (PEDV) is a major causative agent of fatal diarrhea in piglets and is a serious problem in the hog-raising industry. In this study, we assessed the feasibility of producing a protective antigen for the PEDV spike protein 1 using duckweed, Lemna minor. Stably transformed Lemna were obtained by co-cultivation with A. tumefaciens EHA105 harboring the PEDV spike protein gene. Transgene integration and expression of the PEDV spike protein 1 gene were confirmed by genomic PCR and RT-PCR and western blot analysis of transgenic Lemna, respectively. This is the first report of the expression of a vaccine antigen against an animal infectious disease in duckweed.
- Published
- 2011
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