323 results on '"Sue, Shin"'
Search Results
52. The novel <scp>HLA‐DPB1</scp> allele, <scp> HLA ‐ DPB1 </scp> *1344:01 , first identified in Korean individuals by next‐generation sequencing
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Eun Youn Roh, Sori Lim, Sue Shin, and Jong Hyun Yoon
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Immunology ,Genetics ,Immunology and Allergy - Published
- 2022
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53. Effects of Resistance Training and Aerobic Exercise on Insulin Sensitivity in Overweight Korean Adolescents: A Controlled Randomized Trial
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Sunghwan Suh, In-Kyong Jeong, Mi Yeon Kim, Yeon Soo Kim, Sue Shin, Sun Sin Kim, and Jae Hyeon Kim
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Adolescent ,Aerobic exercise ,Insulin sensitivity ,Resistance training ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
BackgroundData on the impact of resistance training on insulin resistance in overweight or obese children are inconclusive.MethodsThirty overweight South Korean adolescents (mean age of 13.10 years) were divided by sex, and then randomly assigned to one of three treatment groups, which were the diet only (DO), diet with aerobic exercise (AE), or diet with resistance training (RT) group. Physiologic and metabolic parameters were assessed at baseline and after 12 weeks of exercise training and diet modification.ResultsBoth exercise groups (aerobic and resistance) showed significant improvements in their insulin area under the curve and insulin sensitivity index values when compared to their baseline values while the DO group showed no significant changes in these variables. Age-, sex-, and body mass index (BMI)-adjusted intergroup comparison analyses showed a marked reduction in BMI and a significant reduction in muscle mass in the AE group when compared to the RT group and the DO group, respectively.ConclusionA 12-week exercise training program of either resistance or aerobic activity improved insulin sensitivity in overweight adolescents, although it failed to show superiority over a DO program. Aerobic exercise decreased both body weight and BMI, and it was noted that this group also had a significant reduction in muscle mass when compared to the DO group.
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- 2011
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54. Clinically refined epidemiology of nontuberculous mycobacterial pulmonary disease in South Korea: overestimation when relying only on diagnostic codes
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Jae Hyeon Park, Sue Shin, Taek Soo Kim, and Hyunwoong Park
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Pulmonary and Respiratory Medicine ,Lung Diseases ,Republic of Korea ,Humans ,Mycobacterium Infections, Nontuberculous ,Female ,Nontuberculous Mycobacteria ,Lung ,Retrospective Studies - Abstract
Background There have been reports of increases in the incidence and prevalence of nontuberculous mycobacterial pulmonary disease (NTM-PD) in several countries, but no studies have analyzed claims data using laboratory tests. This study aimed to estimate the nationwide epidemiology and medical treatments of NTM-PD according to laboratory tests run in Korea. Methods Using claims data from the Health Insurance Review and Assessment Service, we analyzed patients with nontuberculous mycobacterium (ICD-10: A31) who were diagnosed from Jan 2007 to Jun 2019. The incidence and prevalence of NTM-PD and whether related laboratory tests were performed were analyzed. Diagnostic code-based NTM-PD patients were defined as patients who had NTM as a diagnosis on at least 2 occasions within 180 days. Clinically refined NTM-PD patients were defined as those excluding hospital-diagnosed patients with acid-fast bacilli (AFB) culture rates less than 5%. Laboratory tests included AFB smears, AFB culture, NTM identification, and drug susceptibility tests (DSTs). Results A total of 60,071 diagnostic code-based NTM-PD patients were evaluated. Clinically refined NTM-PD included 45,321 patients, excluding 14,750 (24.6%) patients diagnosed in hospitals with low AFB culture rates. The annual incidence per 100,000 population increased from 2.9 cases in 2008 to 12.3 cases in 2018. The annual prevalence per 100,000 population increased from 5.3 cases in 2008 to 41.7 cases in 2018. After removing outliers according to the AFB culture rate, a significant decrease in incidence was observed in women younger than 50 years. Among patients with clinically refined NTM-PD, the test rates for AFB culture, NTM identification, and DST were 84.3%, 59.1%, and 40.4%, respectively. From the outpatient clinic, 17,977 (39.7%) patients were prescribed drugs related to NTM treatment, with a median number of prescriptions of 7 (interquartile range (IQR) 3–11) and a median duration from the diagnosis to end of treatment of 330 (IQR 118–578) days. Conclusions Although the incidence and prevalence of NTM-PD are on the rise, the recent surge in women 50 years of age is overestimated in patients not adequately tested. In claim-based studies, there may be limitations in estimating the epidemiological data with only the diagnostic codes.
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- 2021
55. SARS-CoV-2 Infectivity and Antibody Titer Reduction for 6 Months After Second Dose of BNT162b2 mRNA Vaccine in Health Care Workers: A Prospective Cohort Study
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Namhee Kim, Sue Shin, Dohsik Minn, Seungman Park, Dongheui An, Jae Hyeon Park, Eun Youn Roh, Jong Hyun Yoon, and Hyunwoong Park
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Vaccines, Synthetic ,Infectious Diseases ,SARS-CoV-2 ,Health Personnel ,virus diseases ,Immunology and Allergy ,COVID-19 ,Humans ,Prospective Studies ,mRNA Vaccines ,Antibodies, Viral ,Antibodies, Neutralizing ,BNT162 Vaccine - Abstract
Several studies reported that severe acute respiratory syndrome coronavirus-2 antibody levels change over 6 months in participants receiving the vaccination. From the enrolled 272 health care workers (HCWs), blood samples were obtained at 2, 16, and 24 weeks after the second vaccination dose. In the 267 noninfected HCWs, the neutralizing antibodies decreased by 23.9%, and the anti-spike/receptor binding domain antibody decreased by 53.8% at 24 weeks. We observed no significant difference in antibody reduction between the sexes; however, in younger individuals, there was higher antibody formation and lower reduction rates of the neutralizing antibody. In 3 HCWs with breakthrough infections, the antibody levels were relatively low just before the coronavirus disease 2019 infection. In conclusion, as antibody titers decrease over time after the second vaccination dose and HCWs with low antibody titers tend to have a high probability of breakthrough infection, an additional dose should be considered after several months. Blood samples were obtained from health care workers at 2, 16, and 24 weeks after a second vaccination dose. Antibody titers decreased over time and the participants with low antibody titers tended to have a high probability of breakthrough infection.
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- 2021
56. Natural Killer Cell Expansion and Cytotoxicity Differ Depending on the Culture Medium Used
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Seung Kwon Koh, Jeehun Park, Seong-Eun Kim, Yuree Lim, Minh-Trang Thi Phan, Jinho Kim, Ilwoong Hwang, Yong-Oon Ahn, Sue Shin, Junsang Doh, and Duck Cho
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Killer Cells, Natural ,Biochemistry (medical) ,Clinical Biochemistry ,Humans ,General Medicine ,Cell Proliferation ,Culture Media - Abstract
Adoptive cell therapy using umbilical cord blood (UCB)-derived allogeneic natural killer (NK) cells has shown encouraging results. However, because of the insufficient availability of NK cells and limited UCB volume, more effective culture methods are required. NK cell expansion and functionality are largely affected by the culture medium. While human serum is a major affecting component in culture media, the way it regulates NK cell functionality remains elusive. We elucidated the effects of different culture media and human serum supplementation on UCB NK cell expansion and functionality.UCB NK cells were cultured under stimulation with K562-OX40L-mbIL-18/21 feeder cells and IL-2 and IL-15 in serum-containing and serum-free culture media. The effects of the culture media and human serum supplementation on NK cell expansion and cytotoxicity were evaluated by analyzing the expansion rate, activating and inhibitory receptor levels, and the cytotoxicity of the UCB NK cells.The optimal medium for NK cell expansion was Dulbecco's modified Eagle's medium/Ham's F12 with supplements and that for cytotoxicity was AIM V supplemented with Immune Cell Serum Replacement. Shifting media is an advantageous strategy for obtaining several highly functional UCB NK cells. Live cell imaging and killing time measurement revealed that human serum enhanced NK cell proliferation but delayed target recognition, resulting in reduced cytotoxicity.Culture medium supplementation with human serum strongly affects UCB NK cell expansion and functionality. Thus, culture media should be carefully selected to ensure both NK cell quantity and quality for adoptive cell therapy.
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- 2021
57. Maternal dyslipidemia and altered cholesterol metabolism in early pregnancy as a risk factor for small for gestational age neonates
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Ja Nam Koo, Ig Hwan Oh, Byoung Jae Kim, So Yeon Kim, Go Eun Kwon, Young Mi Jung, Errol R. Norwitz, Chan-Wook Park, Sun Min Kim, Sue Shin, Sae Kyung Joo, Seung Mi Lee, Jong Kwan Jun, Man Ho Choi, Won Kim, and Joong Shin Park
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Adult ,medicine.medical_specialty ,Science ,Lathosterol ,Article ,Pregnancy outcome ,chemistry.chemical_compound ,Pregnancy ,Risk Factors ,Internal medicine ,Humans ,Medicine ,Prospective Studies ,Risk factor ,Prospective cohort study ,Dyslipidaemias ,reproductive and urinary physiology ,Dyslipidemias ,Multidisciplinary ,business.industry ,Cholesterol ,Cholesterol, HDL ,Confounding ,Infant, Newborn ,Intrauterine growth ,medicine.disease ,female genital diseases and pregnancy complications ,Pregnancy Complications ,Pregnancy Trimester, First ,Endocrinology ,chemistry ,Quartile ,Infant, Small for Gestational Age ,Small for gestational age ,lipids (amino acids, peptides, and proteins) ,Female ,business ,Dyslipidemia - Abstract
We evaluated the relationship between maternal cholesterol levels and its biologically active precursors and metabolites in the first trimester and subsequent risk for small-for-gestational-age birthweight (SGA). This is a secondary analysis of a prospective cohort study which enrolled healthy singleton pregnancies (n = 1337). Maternal fasting blood was taken in the first trimester and followed up till delivery. The lipid parameters were compared between women who delivered SGA neonates (SGA-group, birthweight p = 0.022). The risk for SGA was negatively correlated with maternal serum HDL-C quartiles (p = 0.003), and this association remained significant after adjustment for confounding variables. In metabolic signatures of cholesterol, the cholesterol/lathosterol ratio in SGA-group was significantly higher than non-SGA-group [(2.7 (1.6–3.7) vs. 2.1 (1.5–2.9), respectively; p = 0.034)], suggesting increased endogenous cholesterol biosynthesis. We demonstrated that dyslipidemia and increased cholesterol biosynthesis led to delivery of SGA neonates even in early pregnancy.
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- 2021
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58. Attached Segment has Higher CD34 Cells and CFU-GM than the Main Bag after Thawing
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Hye Ryun Lee, Sue Shin, Jong Hyun Yoon M.D., Eun Youn Roh, Eun Young Song, Kyou Sup Han, and Byoung Jae Kim
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Medicine - Abstract
A contiguous segment attached to the cord blood unit (CBU) is required for verifying HLA types, cell viability, and, possibly, potency before transplantation since such a segment is considered to be representative of the CBU. However, little is known regarding the characteristics of contiguous segments in comparison to main bag units due to the difficulty experienced in accessing a large number of cryopreserved CBUs. In this study, we used 245 nonconforming CBUs for allogeneic transplantation. After thawing the cryopreserved CBU, the number of total nucleated cells (TNCs), CD34 + cells, and CFUs in CB from main bags and segments, as well as cell viability and apoptosis, were examined. The comparative analysis showed that the number of TNCs was significantly higher in CB from main bags, whereas the numbers of CD34 + cells and CFU-GM were significantly higher in CB from segments. While the cell viability of TNCs in segments was higher, the proportion of apoptotic TNCs was also higher. In contrast, no difference was observed between the proportion of apoptotic CD34 + cells in main bags and segments. In the correlation analysis, the numbers of TNCs, CD34 + cells, and CFU-GM in main bags were highly correlated with those in segments, indicating that CB from segments is indeed representative of CB in main bags. Taken together, we conclude that segments have higher CD34 + cells and CFU-GM and lower TNCs than the main cryopreserved bag, although the two compartments are highly correlated with each other.
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- 2015
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59. Allele and haplotype frequencies of 11 human leukocyte antigen loci in Koreans by next-generation sequencing
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Inseong Oh, Kiwook Jung, Jisoo G. Kim, Sue Shin, Eun Youn Roh, Yun Ji Hong, and Eun Young Song
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Transplantation ,Immunology - Published
- 2022
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60. Metabolic Dysfunction-Associated Fatty Liver Disease and Subsequent Development of Adverse Pregnancy Outcomes
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Errol R. Norwitz, Soo Heon Kwak, Sang Youn Kim, Eun Saem Choi, Ig Hwan Oh, Seung Mi Lee, Ja Nam Koo, Young Mi Jung, Won Kim, Sun Min Kim, Byoung Jae Kim, Gyoung Min Kim, Sae Kyung Joo, Chan-Wook Park, Jong Kwan Jun, Joong Shin Park, Sue Shin, and Bo Kyung Koo
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medicine.medical_specialty ,digestive system ,Liver disease ,Pregnancy ,Non-alcoholic Fatty Liver Disease ,Internal medicine ,Nonalcoholic fatty liver disease ,medicine ,Humans ,Prospective Studies ,Prospective cohort study ,Hepatology ,business.industry ,Fatty liver ,Infant, Newborn ,Pregnancy Outcome ,Gastroenterology ,nutritional and metabolic diseases ,Odds ratio ,medicine.disease ,digestive system diseases ,Gestational diabetes ,Diabetes, Gestational ,Premature Birth ,Small for gestational age ,Female ,business - Abstract
Background & Aims Recently, metabolic dysfunction-associated fatty liver disease (MAFLD), rather than nonalcoholic fatty liver disease (NAFLD), was proposed to better describe liver disease associated with metabolic dysfunction (MD). In this study, we attempted to investigate the impact of MAFLD on pregnancy complications. Methods The current study is a secondary analysis of a multicenter prospective cohort designed to examine the risk of NAFLD during pregnancy. In the first trimester, enrolled pregnant women were evaluated for hepatic steatosis by liver ultrasonography, and blood samples were collected for biochemical measurements. The study population was divided into three groups: no NAFLD, hepatic steatosis but without metabolic dysfunction (non-MD NAFLD), and MAFLD. The primary outcome was the subsequent development of adverse pregnancy outcomes, including gestational diabetes mellitus, pregnancy-associated hypertension, preterm birth, and fetal growth abnormalities. Results The study population consisted of 1,744 pregnant women, including 1,523 with no NAFLD, 43 with non-MD NAFLD, and 178 with MAFLD. The risk of subsequent development of adverse pregnancy outcomes was higher in MAFLD than in non-MD NAFLD (adjusted odds ratio, 4.03; 95% CI, 1.68-9.67), whereas the risk was not significantly different between no NAFLD and non-MD NAFLD. 3) Among women with no NAFLD, the presence of MD increased the risk of adverse pregnancy outcomes. However, women with MAFLD were at higher risk for adverse pregnancy outcomes than women with no NAFLD without MD or those with no NAFLD with MD. Conclusions In pregnant women, MAFLD may be associated with an increased risk of subsequent adverse pregnancy outcomes.
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- 2022
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61. Development of early prediction model for pregnancy-associated hypertension with graph-based semi-supervised learning
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Seung Mi Lee, Yonghyun Nam, Eun Saem Choi, Young Mi Jung, Vivek Sriram, Jacob S. Leiby, Ja Nam Koo, Ig Hwan Oh, Byoung Jae Kim, Sun Min Kim, Sang Youn Kim, Gyoung Min Kim, Sae Kyung Joo, Sue Shin, Errol R. Norwitz, Chan-Wook Park, Jong Kwan Jun, Won Kim, Dokyoon Kim, and Joong Shin Park
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Multidisciplinary ,Pregnancy ,Humans ,Female ,Hypertension, Pregnancy-Induced ,Prospective Studies ,Supervised Machine Learning ,Biomarkers ,Placenta Growth Factor - Abstract
Clinical guidelines recommend several risk factors to identify women in early pregnancy at high risk of developing pregnancy-associated hypertension. However, these variables result in low predictive accuracy. Here, we developed a prediction model for pregnancy-associated hypertension using graph-based semi-supervised learning. This is a secondary analysis of a prospective study of healthy pregnant women. To develop the prediction model, we compared the prediction performances across five machine learning methods (semi-supervised learning with both labeled and unlabeled data, semi-supervised learning with labeled data only, logistic regression, support vector machine, and random forest) using three different variable sets: [a] variables from clinical guidelines, [b] selected important variables from the feature selection, and [c] all routine variables. Additionally, the proposed prediction model was compared with placental growth factor, a predictive biomarker for pregnancy-associated hypertension. The study population consisted of 1404 women, including 1347 women with complete follow-up (labeled data) and 57 women with incomplete follow-up (unlabeled data). Among the 1347 with complete follow-up, 2.4% (33/1347) developed pregnancy-associated HTN. Graph-based semi-supervised learning using top 11 variables achieved the best average prediction performance (mean area under the curve (AUC) of 0.89 in training set and 0.81 in test set), with higher sensitivity (72.7% vs 45.5% in test set) and similar specificity (80.0% vs 80.5% in test set) compared to risk factors from clinical guidelines. In addition, our proposed model with graph-based SSL had a higher performance than that of placental growth factor for total study population (AUC, 0.71 vs. 0.80, p
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- 2021
62. Evaluation of Three Multiplex Real-time Reverse Transcription PCR Assays for Simultaneous Detection of SARS-CoV-2, Influenza A/B, and Respiratory Syncytial Virus in Nasopharyngeal Swabs
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Jiwon Yun, Jong Hyun Yoon, Jaehyeon Park, Hyunwoong Park, Nam Hee Kim, Sue Shin, Taek Soo Kim, and Eun Youn Roh
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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,viruses ,Respiratory Syncytial Virus Infections ,Biology ,Cross Reactions ,medicine.disease_cause ,Virus ,Viral Matrix Proteins ,Viral Proteins ,Limit of Detection ,Nasopharynx ,Multiplex polymerase chain reaction ,Influenza, Human ,Republic of Korea ,Influenza A virus ,medicine ,Humans ,Multiplex ,Respiratory system ,Polyproteins ,SARS-CoV-2 ,virus diseases ,COVID-19 ,Influenza a ,General Medicine ,Nucleocapsid Proteins ,Infectious Diseases, Microbiology & Parasitology ,Virology ,Influenza B Virus ,Respiratory Syncytial Viruses ,Reverse transcription polymerase chain reaction ,Respiratory Syncytial Virus, Human ,RNA, Viral ,Original Article ,Reagent Kits, Diagnostic ,Multiplex Polymerase Chain Reaction - Abstract
Background In the coronavirus disease 2019 (COVID-19) pandemic era, the simultaneous detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza virus (Flu), and respiratory syncytial virus (RSV) is important in the rapid differential diagnosis in patients with respiratory symptoms. Three multiplex real-time reverse transcription polymerase chain reaction (rRT-PCR) assays have been recently developed commercially in Korea: PowerChek™ SARS-CoV-2, Influenza A&B Multiplex Real-time PCR Kit (PowerChek; KogeneBiotech); STANDARD™ M Flu/SARS-CoV-2 Real-time Detection Kit (STANDARD M; SD BioSensor); and Allplex™ SARS-CoV-2/FluA/FluB/RSV Assay (Allplex; Seegene). We evaluated the analytical and clinical performances of these kits. Methods A limit of detection tests were performed and cross-reactivity analysis was executed using clinical respiratory samples. Ninety-seven SARS-CoV-2-positive, 201 SARS-CoV-2-negative, 71 influenza A-positive, 50 influenza B-positive, 78 RSV-positive, and 207 other respiratory virus-positive nasopharyngeal swabs were tested using the three assays. The AdvanSure™ respiratory viruses rRT-PCR assay (AdvanSure; LG Life Sciences) was used as a comparator assay for RSV. Results Except in influenza B, in SARS-CoV-2 and influenza A, there were no significant differences in detecting specific genes of the viruses among the three assays. All three kits did not cross-react with common respiratory viruses. All three kits had greater than 92% positive percent agreement and negative percent agreement and ≥ 0.95 kappa value in the detection of SARS-CoV-2 and flu A/B. Allplex detected RSV more sensitively than AdvanSure. Conclusion The overall performance of three multiplex rRT-PCR assays for the concurrent detection of SARS-CoV-2, influenza A/B, and RSV was comparable. These kits will promote prompt differential diagnosis of COVID-19, influenza, and RSV infection in the COVID-19 pandemic era., Graphical Abstract
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- 2021
63. Performance evaluation of three automated quantitative immunoassays and their correlation with a surrogate virus neutralization test in coronavirus disease 19 patients and pre-pandemic controls
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Sue Shin, Yun Ji Hong, Minjeong Nam, Kiwook Jung, Eun Young Song, Kyoung Un Park, and Eun Youn Roh
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Microbiology (medical) ,viruses ,Clinical Biochemistry ,Disease ,medicine.disease_cause ,Antibodies, Viral ,Sensitivity and Specificity ,Neutralization ,SARS‐CoV‐2 ,Serology ,COVID-19 Serological Testing ,Antigen ,Neutralization Tests ,spike antigen ,Immunology and Allergy ,Medicine ,Humans ,Serologic Tests ,Neutralizing antibody ,skin and connective tissue diseases ,Research Articles ,Coronavirus ,Immunoassay ,biology ,business.industry ,SARS-CoV-2 ,Biochemistry (medical) ,fungi ,Public Health, Environmental and Occupational Health ,virus diseases ,COVID-19 ,Reproducibility of Results ,neutralizing antibody ,virus neutralization test ,Hematology ,Antibodies, Neutralizing ,Vaccination ,body regions ,immunoassays ,Medical Laboratory Technology ,ROC Curve ,Immunoglobulin G ,Immunology ,Spike Glycoprotein, Coronavirus ,biology.protein ,Antibody ,business ,Research Article - Abstract
Background SARS‐CoV‐2 pandemic is currently ongoing, meanwhile vaccinations are rapidly underway in some countries. The quantitative immunoassays detecting antibodies against spike antigen of SARS‐CoV‐2 have been developed based on the findings that they have a better correlation with the neutralizing antibody. Methods The performances of the Abbott Architect SARS‐CoV‐2 IgG II Quant, DiaSorin LIAISON SARS‐CoV‐2 TrimericS IgG, and Roche Elecsys anti‐SARS‐CoV‐2 S were evaluated on 173 sera from 126 SARS‐CoV‐2 patients and 151 pre‐pandemic sera. Their correlations with GenScript cPass SARS‐CoV‐2 Neutralization Antibody Detection Kit were also analyzed on 173 sera from 126 SARS‐CoV‐2 patients. Results Architect SARS‐CoV‐2 IgG II Quant and Elecsys anti‐SARS‐CoV‐2 S showed the highest overall sensitivity (96.0%), followed by LIAISON SARS‐CoV‐2 TrimericS IgG (93.6%). The specificities of Elecsys anti‐SARS‐CoV‐2 S and LIAISON SARS‐CoV‐2 TrimericS IgG were 100.0%, followed by Architect SARS‐CoV‐2 IgG II Quant (99.3%). Regarding the correlation with cPass neutralization antibody assay, LIAISON SARS‐CoV‐2 TrimericS IgG showed the best correlation (Spearman rho = 0.88), followed by Architect SARS‐CoV‐2 IgG II Quant and Elecsys anti‐SARS‐CoV‐2 S (all rho = 0.87). Conclusions The three automated quantitative immunoassays showed good diagnostic performance and strong correlations with neutralization antibodies. These assays will be useful in diagnostic assistance, evaluating the response to vaccination, and the assessment of herd immunity in the future., The performances of three automated quantitative immunoassays detecting antibodies against SARS‐CoV‐2 spike protein, Abbott SARS‐CoV‐2 IgG II Quant; DiaSorin LIAISON SARS‐CoV‐2 TrimericS IgG; Roche Elecsys anti‐SARS‐CoV‐2 S were comparable, with superior sensitivity and specificity. The three immunoassays demonstrated strong correlations with GenScript cPass virus neutralization test.
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- 2021
64. Antibody microarray analysis of the amniotic fluid proteome for predicting the outcome of rescue cerclage in patients with cervical insufficiency
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Sue Shin, Kyo Hoon Park, Yu Mi Kim, Young Eun Lee, Subeen Hong, and Hyeon Ji Kim
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Adult ,Proteomics ,0301 basic medicine ,Immunology & Inflammation ,medicine.medical_specialty ,Amniotic fluid ,Cervical insufficiency ,Proteome ,Antibody microarray ,Protein Array Analysis ,Biophysics ,Enzyme-Linked Immunosorbent Assay ,Biochemistry ,Gastroenterology ,Molecular Bases of Health & Disease ,03 medical and health sciences ,0302 clinical medicine ,Predictive Value of Tests ,Pregnancy ,Internal medicine ,Humans ,Medicine ,Biomarker discovery ,cervical insufficiency ,Molecular Biology ,Diagnostics & Biomarkers ,Research Articles ,Cerclage, Cervical ,Retrospective Studies ,030219 obstetrics & reproductive medicine ,spontaneous preterm delivery ,business.industry ,Confounding ,Area under the curve ,amniotic fluid ,biomarkers ,Cell Biology ,Treatment Outcome ,030104 developmental biology ,rescue cerclage ,Gene chip analysis ,Gestation ,Female ,Uterine Cervical Incompetence ,business ,antibody microarray - Abstract
Little is known about the biomarkers that can identify patient candidates suitable for rescue cerclage procedure. The purpose of the study was to identify novel biomarkers in amniotic fluid (AF) that can predict the outcome of rescue cerclage in patients with cervical insufficiency by using an antibody microarray. This case–control study was conducted using AF samples collected from singleton pregnant women who underwent rescue cerclage following a diagnosis of cervical insufficiency (19–25 weeks). Patients were divided into case (n=20) and control (n=20) groups based on the occurrence of spontaneous preterm delivery (SPTD) at
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- 2021
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65. Identification of MicroRNA Related to the CD34+ Cell Fraction of Cord Blood Stem Cells
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Hyunwoong Park, Jong Hyun Yoon, Eun Youn Roh, Sue Shin, Joon Hee Lee, Namhee Kim, and Eun Young Song
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business.industry ,Cord blood ,Cd34 cells ,microRNA ,CD34 ,Medicine ,Identification (biology) ,Fraction (chemistry) ,Stem cell ,business ,Cell biology - Published
- 2019
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66. GWAS identifying HLA‐DPB1 gene variants associated with responsiveness to hepatitis B virus vaccination in Koreans: Independent association of HLA‐DPB1*04:02 possessing rs1042169 G ‐ rs9277355 C ‐ rs9277356 A
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Yunhwan Lee, Eun Young Song, Soie Chung, Jong Hyun Yoon, Sue Shin, Eun Youn Roh, and Boram Park
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Hepatitis B virus ,Hepatology ,HLA-DPB1 ,business.industry ,Haplotype ,Genome-wide association study ,Single-nucleotide polymorphism ,Hepatitis B ,medicine.disease_cause ,medicine.disease ,Vaccination ,03 medical and health sciences ,0302 clinical medicine ,Infectious Diseases ,Virology ,Immunology ,Genotype ,Medicine ,030211 gastroenterology & hepatology ,030212 general & internal medicine ,business - Abstract
Recently, HLA class II loci, including HLA-DPB1, have been reported to be associated with interindividual variance in the hepatitis B (HB) vaccine response. In this study, we investigated significant single nucleotide polymorphisms (SNPs) for anti-HBs antibody levels in 6867 healthy Koreans using a genome-wide association study (GWAS). In GWAS, the top 20 SNPs that showed significant association with anti-HBs levels (P
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- 2019
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67. Chronic Rhinosinusitis without Nasal Polyps in Asian Patients Shows Mixed Inflammatory Patterns and Neutrophil-Related Disease Severity
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Dong-Kyu Kim, Kyoung Mi Eun, Dae Woo Kim, Sue Shin, and Eun Youn Roh
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Adult ,Male ,Chemokine ,Article Subject ,Neutrophils ,Immunology ,Inflammation ,Severity of Illness Index ,Type 2 immune response ,Interferon-gamma ,03 medical and health sciences ,Nasal Polyps ,0302 clinical medicine ,Immune system ,Asian People ,Severity of illness ,Eosinophilic ,lcsh:Pathology ,medicine ,Humans ,Nasal polyps ,Sinusitis ,030223 otorhinolaryngology ,Rhinitis ,biology ,business.industry ,Case-control study ,Endoscopy ,Cell Biology ,Middle Aged ,medicine.disease ,Eosinophils ,030228 respiratory system ,Case-Control Studies ,Chronic Disease ,biology.protein ,Cytokines ,Female ,Chemokines ,medicine.symptom ,business ,Research Article ,lcsh:RB1-214 - Abstract
Chronic rhinosinusitis (CRS) shows heterogeneous immunologic features. Western studies revealed that CRS without nasal polyps (CRSsNP) showed a predominantly type 1 immune response and CRS with nasal polyps (CRSwNP) was characterized by type 2 immune response; however, the detailed immunologic profile of CRSsNP in Asian patients has not been thoroughly investigated. Therefore, we investigated the inflammatory endotypes of CRSsNP in Asian patients. Patients with CRSsNP (N=57), patients with CRSwNP (N=13), and a control group (N=10), who underwent endoscopic sinus surgery, were enrolled; uncinate process (UP) tissues were harvested from all patients. Homogenates were prepared from the UP of each group, and immunologic profiles were analyzed, including major cytokines (32 inflammatory mediators). When comparing the UPs between groups, CRSsNP patients showed higher levels of Th2 cytokines (IL-4 and IL-13), eosinophilic chemokines (CCL-11 and CCL-24), ECP, and total IgE expression than control subjects. In addition, several neutrophilic markers (IL-1α, IL-6, IL-8, CXCL-1, CXCL-2, and MPO), IL-17A, IL-22, and TNF-α were dominant in CRSsNP patients. Among these inflammatory mediators, IL-17A showed higher expression levels in CRSsNP patients than in the control group and CRSwNP patients. However, IFN-γ expression was not significantly elevated in CRSsNP patients. The levels of neutrophil-associated cytokines were well correlated with each other; of which, CXCL2, IL-8, and MMP-9/TIMP-1 levels were significantly correlated with disease extent (r=0.338, r=0.317, and r=0.424, respectively). However, the levels of eosinophil-associated cytokines showed little correlation with each other and were not correlated with disease extent. Our study revealed that Asian CRSsNP patients showed a mixed (types 2 and 17) immune response, but neutrophil-related markers were dominant and associated with disease extent. Knowledge of this immunologic feature may help clinicians make better individual treatment decisions for Asian CRSsNP patients.
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- 2019
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68. MBP-positive and CD11c-positive cells are associated with different phenotypes of Korean patients with non-asthmatic chronic rhinosinusitis.
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Dong-Kyu Kim, Min-Hyun Park, Dong-Yeop Chang, Kyung Mi Eun, Hyun-Woo Shin, Ji-Hun Mo, Eui-Cheol Shin, Hong Ryul Jin, Sue Shin, Eun Youn Roh, Doo Hee Han, and Dae Woo Kim
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Medicine ,Science - Abstract
BACKGROUND: Asthmatic nasal polyps primarily exhibit eosinophilic infiltration. However, the identities of the immune cells that infiltrate non-asthmatic nasal polyps remain unclear. Thus, we thought to investigate the distribution of innate immune cells and its clinical relevance in non-asthmatic chronic rhinosinusitis (CRS) in Korea. METHODS: Tissues from uncinate process (UP) were obtained from controls (n = 18) and CRS without nasal polyps (CRSsNP, n = 45). Nasal polyps (NP) and UP were obtained from CRS with nasal polyps (CRSwNP, n = 56). The innate immune cells was evaluated by immunohistochemistry such as, eosinophil major basic protein (MBP), tryptase, CD68, CD163, CD11c, 2D7, human neutrophil elastase (HNE) and its distribution was analyzed according to clinical parameters. RESULTS: In comparisons between UP from each group, CRSwNP had a higher number of MPB(+), CD68(+), and CD11c(+) cells relative to CRSsNP. Comparisons between UP and NP from CRSwNP indicated that NP have a higher infiltrate of MBP(+), CD163(+), CD11c(+), 2D7(+) and HNE(+) cells, whereas fewer CD68(+) cells were found in NP. In addition, MBP(+) and CD11c(+) cells were increased from UP of CRSsNP, to UP of CRSwNP, and to NP of CRSwNP. Moreover, in UP from CRSwNP, the number of MBP(+) and CD11c(+) cells positively correlated with CT scores. In the analysis of CRSwNP phenotype, allergic eosinophilic polyps had a higher number of MBP(+), tryptase(+), CD11c(+), 2D7(+) cells than others, whereas allergic non-eosinophilic polyps showed mainly infiltration of HNE(+) and 2D7(+) cells. CONCLUSIONS: The infiltration of MBP(+) and CD11c(+) innate immune cells show a significant association with phenotype and disease extent of CRS and allergic status also may influences cellular phenotype in non-asthmatic CRSwNP in Korea.
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- 2014
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69. Positivity of SARS-CoV-2 Antibodies among Korean Healthy Healthcare Workers 1 and 2 Weeks after Second Dose of Pfizer-BioNTech Vaccination
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Jong Hyun Yoon, Dohsik Minn, Namhee Kim, Eun Youn Roh, Seungman Park, Hyunwoong Park, and Sue Shin
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COVID-19 Vaccines ,Time Factors ,Coronavirus disease 2019 (COVID-19) ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,viruses ,Health Personnel ,Korean ,Brief Communication ,Antibodies, Viral ,03 medical and health sciences ,0302 clinical medicine ,Medicine ,Humans ,030212 general & internal medicine ,BNT162 Vaccine ,Whole blood ,Immunoassay ,medicine.diagnostic_test ,biology ,business.industry ,SARS-CoV-2 ,Vaccination ,Antibody titer ,COVID-19 ,General Medicine ,mRNA Vaccine ,Laboratory Medicine ,Immunology ,Antibody Formation ,Luminescent Measurements ,biology.protein ,Antibody ,business ,Antibody formation - Abstract
The antibody titer of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was observed in 289 healthy healthcare workers who had completed the second dose of the Pfizer-BioNTech coronavirus disease 2019 (COVID-19) vaccine. Antibody tests were performed using both the automated electrochemiluminescence immunoassay (ECLIA) and the chromatographic lateral flow immunoassay (LFIA). All subjects had antibodies against the receptor binding domain of the spike protein of SARS-CoV-2 only one week after completing the vaccination, and the antibody titer became significantly higher after another week (P < 0.001). Since there was a large amount of antibody formation within two weeks after completion of vaccination, the less sensitive method, LFIA, also showed high sensitivity. There was no significant difference between whole blood and serum in detecting SARS-CoV-2 antibodies after vaccination. This is an early study of vaccinations among Koreans and is expected to contribute to the establishment of national guidelines on COVID-19 vaccination., Graphical Abstract
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- 2021
70. Natural Killer Cell Expansion and Cytotoxicity Differ Depending on the Culture Medium Used.
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Seung Kwon Koh, Jeehun Park, Seong-Eun Kim, Yuree Lim, Minh-Trang Thi Phan, Jinho Kim, Ilwoong Hwang, Yong-Oon Ahn, Sue Shin, Junsang Doh, and Duck Cho
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CORD blood ,SERUM-free culture media ,IMMUNE serums ,CELL imaging ,KILLER cells ,ANTIBODY-dependent cell cytotoxicity - Abstract
Background: Adoptive cell therapy using umbilical cord blood (UCB)-derived allogeneic natural killer (NK) cells has shown encouraging results. However, because of the insufficient availability of NK cells and limited UCB volume, more effective culture methods are required. NK cell expansion and functionality are largely affected by the culture medium. While human serum is a major affecting component in culture media, the way it regulates NK cell functionality remains elusive. We elucidated the effects of different culture media and human serum supplementation on UCB NK cell expansion and functionality. Methods: UCB NK cells were cultured under stimulation with K562-OX40L-mbIL-18/21 feeder cells and IL-2 and IL-15 in serum-containing and serum-free culture media. The effects of the culture media and human serum supplementation on NK cell expansion and cytotoxicity were evaluated by analyzing the expansion rate, activating and inhibitory receptor levels, and the cytotoxicity of the UCB NK cells. Results: The optimal medium for NK cell expansion was Dulbecco's modified Eagle's medium/Ham's F12 with supplements and that for cytotoxicity was AIM V supplemented with Immune Cell Serum Replacement. Shifting media is an advantageous strategy for obtaining several highly functional UCB NK cells. Live cell imaging and killing time measurement revealed that human serum enhanced NK cell proliferation but delayed target recognition, resulting in reduced cytotoxicity. Conclusions: Culture medium supplementation with human serum strongly affects UCB NK cell expansion and functionality. Thus, culture media should be carefully selected to ensure both NK cell quantity and quality for adoptive cell therapy. [ABSTRACT FROM AUTHOR]
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- 2022
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71. Evaluation of LabType-SSO HLA Typing for HLA-A, -B, -C, -DRB1, and -DQB1 loci
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Kyoung Un Park, Minjeong Nam, Eun Young Song, Kiwook Jung, Eun Youn Roh, and Sue Shin
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Genetics ,HLA-A Antigens ,Histocompatibility Testing ,Oligonucleotides ,Human leukocyte antigen ,Biology ,General Biochemistry, Genetics and Molecular Biology ,HLA-A ,Gene Frequency ,Haplotypes ,Hla genotyping ,Humans ,Typing ,Allele ,Solid organ transplantation ,Allele frequency ,Alleles ,HLA-DRB1 Chains - Abstract
Background For HLA genotyping, PCR sequence-specific oligonucleotide (SSO) methods using the Luminex platform are widely used. We evaluated the performance of LabType-SSO (One Lambda, USA) in Koreans. Methods LabType-SSO were performed on 50 residual DNA samples analyzed by sequence-based typing (SBT) for all HLA-A, -B, -C, -DRB1, and -DQB1 alleles with gene frequency > 0.1% in Koreans. Results The LabType-SSO results were in complete agreement with SBT at the 2-digit level. For 4-digit level, 9 HLA-A alleles, 1 HLA-B allele, 3 HLA-C alleles, neither HLA-DRB1 nor -DQB1 allele showed ambiguous results for assignment of most probable types considering HLA gene frequency in Koreans. In addition, two cases of DQB1*04:01 allele were incorrectly assigned to DQB1*04:02. Conclusions LabType-SSO tests showed accurate assignment of 2-digit level and LabType-SSO HLA-DRB1 test showed correct 4-digit most probable HLA type. The tests can be useful as intermediate resolution typing for solid organ transplantation.
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- 2021
72. Effects of corticosteroids and platelet-rich plasma on synoviocytes in IL-1ß-induced inflammatory condition
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Chris Hyunchul Jo, Sue Shin, Kang Sup Yoon, and Ji Hye Yea
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medicine.medical_specialty ,Anabolism ,medicine.drug_class ,0206 medical engineering ,Anti-Inflammatory Agents ,02 engineering and technology ,Biochemistry ,03 medical and health sciences ,Rheumatology ,Adrenal Cortex Hormones ,Internal medicine ,Synovitis ,medicine ,Orthopedics and Sports Medicine ,Molecular Biology ,Cells, Cultured ,030304 developmental biology ,0303 health sciences ,business.industry ,Platelet-Rich Plasma ,Cell Biology ,medicine.disease ,020601 biomedical engineering ,Synoviocytes ,Endocrinology ,Platelet-rich plasma ,Corticosteroid ,Cytokines ,business - Abstract
Corticosteroid injections are used to treat shoulder pain. Platelet-rich plasma (PRP) is known to have anti-inflammatory and anabolic effects, as well as cytoprotective effects against corticosteroids. Thus, this study was to investigate the effects of co-treatment of corticosteroid and PRP on anti-inflammatory and matrix homeostasis of synoviocytes in IL-1ß-induced inflammatory conditions.Synoviocytes were cultured with 1 ng/mL IL-1β, 1 μM dexamethasone, and 10% (vol/vol) Platelet-poor plasma (PPP), PRP200, PRP1000, and PRP4000 X 10Corticosteroid modulated anti-inflammatory and pro-inflammatory cytokines, and subsequent PRP treatment did not interfere with the effect of a corticosteroid and modulated the gene expressions of cytokines such as TNF-α and IL-4, which were not regulated by the corticosteroid alone. Gene expressions and protein expressions of degradative enzymes and their inhibitors were suppressed by corticosteroid. Additional PRPs did not alter the gene expression and protein regulated by the corticosteroid and inhibited the gene expression of ADAMTS-5 and protein synthesis of MMP-9 and ADAMTS-5, which were not modulated by the corticosteroid alone.Corticosteroid regulated the inflammation and synovial homeostasis. When PRP and the corticosteroid were used together, it exhibited synergistic effects on synoviocytes by regulating the parts that were not controlled by corticosteroid alone while not interfering with the effects of the corticosteroid in an inflammatory condition.
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- 2021
73. Proteomic identification of novel plasma biomarkers associated with spontaneous preterm birth in women with preterm labor without infection/inflammation
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Yu Mi Kim, Ji-Woong Choi, Hyeon Ji Kim, Kyong-No Lee, Kyo Hoon Park, Ji Eun Lee, and Sue Shin
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Proteomics ,Proteome ,Physiology ,Maternal Health ,Biochemistry ,Labor and Delivery ,Pregnancy ,Blood plasma ,Medicine and Health Sciences ,Receptor ,Immune Response ,Multidisciplinary ,medicine.diagnostic_test ,Acute-phase protein ,Obstetrics and Gynecology ,Hematology ,Blood proteins ,Body Fluids ,Blood ,Amniocentesis ,Premature Birth ,Medicine ,Female ,medicine.symptom ,Anatomy ,Coagulation Factors ,Research Article ,Adult ,Science ,Immunology ,Inflammation ,Preterm Birth ,Blood Plasma ,Andrology ,Signs and Symptoms ,medicine ,Calgranulin B ,Humans ,Blood Coagulation ,Plasma Proteins ,business.industry ,Biology and Life Sciences ,Proteins ,Factor V ,Retrospective cohort study ,Pregnancy Complications ,Birth ,Women's Health ,Farnesoid X receptor ,Clinical Medicine ,business ,Biomarkers - Abstract
Objective We sought to identify plasma biomarkers associated with spontaneous preterm birth (SPTB, delivery within 21 days of sampling) in women with preterm labor (PTL) without intra-amniotic infection/inflammation (IAI) using label-free quantitative proteomic analysis, as well as to elucidate specific protein pathways involved in these cases. Methods This was a retrospective cohort study comprising 104 singleton pregnant women with PTL (24–32 weeks) who underwent amniocentesis and demonstrated no evidence of IAI. Analysis of pooled plasma samples collected from SPTB cases and term birth (TB) controls (n = 10 for each group) was performed using label-free quantitative mass spectrometry for proteome profiling in a nested case-control study design. Eight candidate proteins of interest were validated by ELISA-based assay and a clot-based assay in the total cohort. Results Ninety-one proteins were differentially expressed (P < 0.05) in plasma samples obtained from SPTB cases, of which 53 (58.2%) were upregulated and 38 (41.8%) were downregulated when compared to TD controls. A validation study confirmed that plasma from women who delivered spontaneously within 21 days of sampling contained significantly higher levels of coagulation factor Ⅴ and lower levels of S100 calcium binding protein A9 (S100A9), especially the former which was independent of baseline variables. The top-ranked pathways related to the 91 differentially expressed proteins were liver-X-receptor/retinoid X receptor (RXR) activation, acute phase response signaling, farnesoid X receptor/RXR activation, coagulation system, and complement system. Conclusions Proteomic analyses in this study identified potential novel biomarkers (i.e., coagulation factor V and S100A9) and potential protein pathways in plasma associated with SPTB in the absence of IAI in women with PTL. The present findings provide novel insights into the molecular pathogenesis and therapeutic targets specific for idiopathic SPTB.
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- 2021
74. Performance evaluation of immunoassay for infectious diseases on the Alinity i system
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Sang Hoon Song, Sue Shin, Eun Young Song, Da Young Song, Minjeong Nam, Kyoung Un Park, and Eun Youn Roh
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0301 basic medicine ,Microbiology (medical) ,Analyte ,HBsAg ,analytical performance ,infectious disease ,Clinical Biochemistry ,Alinity i system ,Cytomegalovirus ,Infections ,Rubella ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Immunology and Allergy ,Humans ,Serologic Tests ,Syphilis ,Research Articles ,Immunoassay ,Hepatitis B Surface Antigens ,medicine.diagnostic_test ,business.industry ,comparison study ,Biochemistry (medical) ,Public Health, Environmental and Occupational Health ,virus diseases ,Reproducibility of Results ,Hematology ,Repeatability ,medicine.disease ,Medical Laboratory Technology ,030104 developmental biology ,HBeAg ,Infectious disease (medical specialty) ,030220 oncology & carcinogenesis ,Immunoglobulin G ,Immunology ,business ,Toxoplasma ,Research Article - Abstract
Background Although a diagnosis of infectious diseases is essential for timely treatment, the performance of diagnostic tests has been hardly evaluated due to variable results that are influenced by multiple factors in different conditions. In the present study, the performance of the Alinity i system, which is a newly developed immunoassay to diagnose infectious diseases, was evaluated. Methods We evaluated the precision, linearity, correlation, and carryover of 16 analytes (HAV Ab IgG, HBsAg, HBeAg, anti‐HBc, anti‐HBe, anti‐HBs, anti‐HCV, HIV Ag/Ab, EBV VCA IgM, EBV VCA IgG, EBV EBNA IgG, CMV IgM, CMV IgG, Toxoplasma IgG, Rubella IgG, and Syphilis TP) of Alinity i by comparison with ARCHITECT i2000SR system following the rationale of the Clinical and Laboratory Standards Institute (CLSI). Results For quantitative tests, the coefficients of variation (CV) % of repeatability and intermediate precision were between 0% and 4.18%. The coefficients of the linearity (r 2) over a widely tested analytical range were ≥ 0.990 and the correlation between Alinity i and the ARCHITECT i2000SR system was strong (r ≥ 0.994). For qualitative tests, the agreement between Alinity i and the ARCHITECT i2000SR system was excellent (kappa coefficient 1) with 100% sensitivity and specificity. Carryover rates for all analytes were less than 1.0% (−0.11% ~ 0.21%). Conclusion The Alinity i system showed good analytical performance and favorable comparability with the ARCHITECT i2000SR. It could be suitable as a routine immunoassay analyzer for screening and diagnosis of infectious disease., For both qualitative and quantitative measurements, the Alinity i system showed good analytical precision and excellent agreement with ARCHITECT i2000SR system. Alinity i system would be an excellent routine immunoassay analyzer for screening and diagnosing infectious disease.
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- 2020
75. Effects of Storage Temperature and Media/Buffer for SARS-CoV-2 Nucleic Acid Detection
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Sue Shin, Namhee Kim, Hyunwoong Park, Mi Seon Han, Jong Hyun Yoon, Ahrin Kwon, Eun Youn Roh, and Sang Won Park
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0301 basic medicine ,Nucleic acid quantitation ,Coronavirus disease 2019 (COVID-19) ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Buffers ,Real-Time Polymerase Chain Reaction ,medicine.disease_cause ,Buffer (optical fiber) ,Specimen Handling ,03 medical and health sciences ,0302 clinical medicine ,Molecular diagnostics ,medicine ,Humans ,030212 general & internal medicine ,Food science ,Coronavirus ,SARS-CoV-2 ,Reverse Transcriptase Polymerase Chain Reaction ,Chemistry ,Temperature ,COVID-19 ,Shipping and storage ,General Medicine ,Media and buffer ,030104 developmental biology ,COVID-19 Nucleic Acid Testing ,Nucleic acid ,RNA, Viral ,Original Article ,Sample collection ,AcademicSubjects/MED00690 ,Nucleic acid detection - Abstract
Objectives The increase in the number of patients with coronavirus disease 2019 (COVID-19) has delayed real-time reverse transcription–quantitative polymerase chain reaction (RT-qPCR), requiring proper shipping and storage conditions, especially in hot weather. This study aims to assess how some conditions, such as storage period, temperature, media or buffer, and sample types, affect the results of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RT-qPCR. Methods SARS-CoV-2–positive specimens were collected from Boramae Medical Center for 2 months (from May to June 2020) and stored in different media or buffers at different temperatures. Results As a result of examining confirmed patient samples, RT-qPCR results were not significantly affected by 2°C to 8°C storage until after 7 days. When stored at 20°C to 22°C or above 35°C, the results were affected negatively even after 1 day. Higher storage temperatures resulted in a lower probability of detecting viral nucleic acids because of degradation. Samples stored in pH-controlled media or buffer were more stable than those stored in nonbuffer states. Conclusions These results emphasize the importance of storage temperature and media or buffer and performing RT-qPCR for SARS-CoV-2 nucleic acid detection as soon as possible after sample collection.
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- 2020
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76. Umbilical Cord Blood Units Cryopreserved in the Public Cord Blood Bank: A Breakthrough in iPSC Haplobanking?
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Sue Shin, Byoung Jae Kim, Eun Young Song, Jong Hyun Yoon, Sohee Oh, and Eun Youn Roh
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0301 basic medicine ,Cord Blood ,Population ,Induced Pluripotent Stem Cells ,Biomedical Engineering ,lcsh:Medicine ,Korean ,Human leukocyte antigen ,Biology ,Umbilical cord ,homozygous ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Allele ,education ,Induced pluripotent stem cell ,Cryopreservation ,Transplantation ,education.field_of_study ,haplobanking ,Haplotype ,lcsh:R ,Cell Biology ,Fetal Blood ,human leukocyte antigen (HLA) ,030104 developmental biology ,medicine.anatomical_structure ,Cord blood ,Immunology ,iPSC (induced pluripotent stem cell) ,Blood Banks ,Original Article ,030215 immunology - Abstract
The use of induced pluripotent stem cells (iPSCs) is an emerging therapeutic option for precision medicine. Cord blood (CB) cells with lower immunogenicity, fewer genomic changes, and persistent epigenetic memory might be ideal candidates for iPSC production. Based on the human leukocyte antigen (HLA) distribution of cord blood units (CBUs) in the public CB bank, we estimated the coverage of the Korean population with HLA-homozygous iPSCs to repurpose cryopreserved CBUs. We analyzed a total of 27,904 Korean CBUs donated to the public CB bank. Low-to-intermediate resolution typing was performed for HLA-A, -B, and -DRB1 alleles, and individuals possessing homozygous HLA haplotypes were identified by direct counting. Moreover, the matching probabilities for zero-mismatch transplantation were calculated for 27,904 CBUs and 50,000,000 potential Korean patients. Among the preserved CBUs, 15 HLA-A, 40 HLA-B, and 13 HLA-DRB1 alleles as well as 48 homozygous HLA-A-B-DRB1 haplotypes were identified at serological equivalents (2 digits). The 48 identified homozygous haplotypes cumulatively matched 78.18% of the 27,904 Korean CB donors as zero HLA-mismatch iPSC sources. Among the combinations of 1,699 haplotypes with frequencies greater than 0.001%, assuming a population of 50 million, those 48 haplotypes can provide a match for 78.37% of potential Korean recipients. A practicable number of HLA-A, -B, and -DRB1 homozygous iPSC lines derived from CBUs may be an efficient option in allogeneic iPSC therapy because this type of haplobanking may provide cell lines with optimal HLA matching for up to three-quarters of the Korean population.
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- 2020
77. Viral RNA Load in Mildly Symptomatic and Asymptomatic Children with COVID-19, Seoul, South Korea
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Sung Sup Park, Sung Im Cho, Nam Hee Kim, Eun Hwa Choi, Taek-Soo Kim, Mi Seon Han, Sue Shin, Moon Woo Seong, and Hyunwoong Park
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Saliva ,Epidemiology ,coronavirus ,lcsh:Medicine ,Urine ,medicine.disease_cause ,Feces ,Plasma ,0302 clinical medicine ,fluids and secretions ,Nasopharynx ,030212 general & internal medicine ,Child ,Asymptomatic Infections ,Coronavirus ,Viral RNA Load in Mildly Symptomatic and Asymptomatic Children with COVID-19, Seoul ,biology ,Transmission (medicine) ,Viral Load ,Infectious Diseases ,coronavirus disease ,Child, Preschool ,RNA, Viral ,medicine.symptom ,Coronavirus Infections ,Viral load ,severe acute respiratory syndrome coronavirus 2 ,Microbiology (medical) ,Adolescent ,Seoul ,030231 tropical medicine ,Pneumonia, Viral ,Asymptomatic ,lcsh:Infectious and parasitic diseases ,03 medical and health sciences ,Betacoronavirus ,respiratory infections ,stomatognathic system ,children ,South Korea ,Republic of Korea ,medicine ,Research Letter ,Humans ,lcsh:RC109-216 ,viruses ,Pandemics ,business.industry ,SARS-CoV-2 ,lcsh:R ,Infant, Newborn ,RNA ,COVID-19 ,Infant ,biology.organism_classification ,Virology ,zoonoses ,business - Abstract
Along with positive SARS-CoV-2 RNA in nasopharyngeal swabs, viral RNA was detectable at high concentration for >3 weeks in fecal samples from 12 mildly symptomatic and asymptomatic children with COVID-19 in Seoul, South Korea. Saliva also tested positive during the early phase of infection. If proven infectious, feces and saliva could serve as transmission sources.
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- 2020
78. Fifteen new nucleotide substitutions in variants of human papillomavirus 18 in Korea
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Jeong Su Park, Nam Hee Kim, Eun Youn Roh, Hyunwoong Park, Ji Eun Kim, Jong Hyun Yoon, Jae Hyeon Park, and Sue Shin
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Adult ,0301 basic medicine ,Lineage (genetic) ,Genotype ,viruses ,Short Report ,Cervix Uteri ,Biology ,Cervical intraepithelial neoplasia ,03 medical and health sciences ,0302 clinical medicine ,Lineage ,Virology ,Republic of Korea ,Prevalence ,medicine ,Carcinoma ,Humans ,Missense mutation ,Gene ,Phylogeny ,Human papillomavirus (HPV) 18 ,Cervical cancer ,Human papillomavirus 18 ,Nucleotides ,Papillomavirus Infections ,Variants ,Genetic Variation ,Middle Aged ,Uterine Cervical Dysplasia ,Vaccine efficacy ,medicine.disease ,E6, E7 and L1 genes ,030104 developmental biology ,Infectious Diseases ,Amino Acid Substitution ,030220 oncology & carcinogenesis ,DNA, Viral ,Female ,Ascus - Abstract
High-risk human papillomavirus (HPV) infection is an essential factor for the development of cervical cancer. HPV18 is the second most common carcinogenic HPV type following HPV16, but the lineages of HPV18 have been less well studied than those of HPV 16. The purpose of this study was to analyze the nucleotide variants in the E6, E7, and L1 genes of HPV18, to assess the prevalence of HPV18 variants in Korea and to explore the relationship between HPV18 genetic variants and the risk for cervical cancer.A total of 170 DNA samples from HPV18-positive cervical specimens were collected from women admitted to a secondary referral hospital located in Seoul. Among them, the lineages of the 97 samples could be successfully determined by historical nomenclature.All the studied HPV 18 variants were lineage A. Sublineages A1 and A4 comprised 91.7% (89/97) and 1.0% (1/97), respectively. Sublineages other than A1 or A4 comprised 7.2% (7/97). We identified 15 new nucleotide substitutions among 44 nucleotide substitutions: C158T, T317G, T443G, A560G, A5467G, A5560C, A5678C, A6155G, G6462A, T6650G, G6701A, T6809C, A6823G, T6941C and T6953C. Among them, 6 substitutions at positions 317, 443, 5467, 5560, 6462, and 6823 resulted in amino acid changes (E6: F71L and N113K; L1: H13R, H44P, A345T, and N465S, respectively). The pathologic results were classified as normal in 25.8% (25/97) of the women, atypical squamous cells of undermined significance (ASCUS) in 7.2% (7/97), cervical intraepithelial neoplasia (CIN) 1 in 36.1% (35/97), CIN2/3 in 19.6% (18/97), and carcinoma in 12.4% (12/97). There was no significant association between the HPV18 sublineages and the severity of pathologic lesion or the disease progression.This study is the first to analyze the distribution of HPV18 variants in Korean and to associate the results with pathologic findings. Although the HPV18 variants had no significant effect on the degree and progression of the disease, the newly discovered nonsynonymous mutation in L1 might serve as a database to determine vaccine efficacy in Korean women.
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- 2020
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79. Associations of Anti-MHC Class I-related Chain A (MICA) Antibodies with Renal Allograft Outcomes
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Nuri, Lee, Minjeong, Nam, Eun Youn, Roh, Sue, Shin, Kyoung Un, Park, and Eun Young, Song
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Graft Rejection ,Male ,Antibody Formation ,Graft Survival ,Histocompatibility Antigens Class I ,Humans ,Female ,Middle Aged ,Allografts ,Prognosis ,Kidney Transplantation ,Antibodies ,Tissue Donors - Abstract
The association of anti-MHC class I-related chain A (MICA) and kidney allograft rejection has been reported. However, the associations of antibodies specific to donor MICA (dsMICA) and dsMICA levels with allograft outcome have not been elucidated. From November 2009 to June 2017, 125 sera samples from renal transplantation recipients with no HLA antibodies were screened for MICA antibodies. Patients with positive MICA antibody screening results and available residual donor DNA for MICA geno-typing underwent dsMICA analysis. Among these 125 sera samples, 19 were positive for MICA antibodies. dsMICA was positive in 5 out of the 12 analyzed sera samples. Neither MICA antibodies nor dsMICA were associated with acute rejection. However, interstitial fibrosis and tubular atrophy (IFTA) was significantly associated with MICA antibody positivity (OR=3.84
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- 2020
80. Sequential Analysis of Viral Load in a Neonate and Her Mother Infected With Severe Acute Respiratory Syndrome Coronavirus 2
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Sung Im Cho, Ji Hong Park, Sue Shin, Namhee Kim, Moon Woo Seong, Mi Seon Han, Sung Sup Park, Eun Hwa Choi, and Eun Young Heo
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Microbiology (medical) ,Saliva ,Coronavirus disease 2019 (COVID-19) ,viruses ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,coronavirus ,Mothers ,Urine ,010501 environmental sciences ,medicine.disease_cause ,01 natural sciences ,Feces ,03 medical and health sciences ,fluids and secretions ,0302 clinical medicine ,stomatognathic system ,Nasopharynx ,otorhinolaryngologic diseases ,medicine ,Humans ,030212 general & internal medicine ,0105 earth and related environmental sciences ,Coronavirus ,Korea ,SARS-CoV-2 ,business.industry ,Brief Report ,Infant, Newborn ,COVID-19 ,RNA ,Viral Load ,Virology ,Body Fluids ,stomatognathic diseases ,AcademicSubjects/MED00290 ,Infectious Diseases ,Vomiting ,RNA, Viral ,Female ,neonate ,medicine.symptom ,business ,Viral load - Abstract
We report changes in viral load over time in a 27-day-old neonate with coronavirus disease 2019 who presented with fever, cough, and vomiting. Severe acute respiratory syndrome coronavirus 2 RNA was detected in the nasopharynx, oropharynx, stool, saliva, plasma, and urine. The highest viral RNA copies in nasopharynx decreased over time while viral load in stool remained high.
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- 2020
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81. Acceptable Donor-Specific Antibody Levels Before and After Desensitization Therapy in Living Donor Kidney Transplantation
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Eun Youn Rho, Ji Won In, Yun Ji Hong, Kyoung Un Park, Sue Shin, Minjeong Nam, and Eun Young Song
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Graft Rejection ,medicine.medical_specialty ,medicine.medical_treatment ,Gastroenterology ,Living donor ,General Biochemistry, Genetics and Molecular Biology ,Antibodies ,HLA Antigens ,Internal medicine ,medicine ,Living Donors ,Humans ,Kidney transplantation ,Desensitization (medicine) ,biology ,business.industry ,Donor specific antibodies ,Immunoglobulins, Intravenous ,Plasmapheresis ,medicine.disease ,Kidney Transplantation ,body regions ,Desensitization, Immunologic ,biology.protein ,Rituximab ,Antibody ,business ,Desensitization therapy ,medicine.drug - Abstract
Background Plasmapheresis (PP) is commonly used for desensitization in highly sensitized patients with donor-specific antibodies (DSA) in living donor kidney transplantation. We analyzed the impact of DSA levels before and after desensitization on renal allograft outcome. Methods Twenty-three patients who underwent desensitization with PP, intravenous immunoglobulin (IVIG), and rituximab before kidney transplantation in Seoul National University Hospital from August 2006 to August 2016 were enrolled. The association of median fluorescent intensity (MFI) value of DSA with graft outcome was analyzed. Results The frequency of positive HLA class II DSA after desensitization was lower in patients without antibody-mediated rejection (AMR) compared to those with AMR (p = 0.006). The cutoff value of MFI sum of HLA class II DSA after desensitization for predicting AMR was 2,122 with 63% sensitivity and 94% specificity. The frequency of moderate HLA class II DSA (MFI 5,000 - 10,000) after desensitization was significantly higher in patients with graft loss compared to those without graft loss (p = 0.02). Conclusions Weak HLA class II DSA after desensitization including PP, IVIG, and rituximab was related to AMR and moderate levels of HLA class II DSA after desensitization was related to graft loss in living donor kidney transplantation.
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- 2020
82. A protein microarray analysis of amniotic fluid proteins for the prediction of spontaneous preterm delivery in women with preterm premature rupture of membranes at 23 to 30 weeks of gestation
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Kyo Hoon Park, Kwanghee Ahn, Eunwook Joo, Sue Shin, Hyeon Ji Kim, and Yu Mi Kim
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Fetal Membranes, Premature Rupture ,Amniotic fluid ,Antibody microarray ,Physiology ,Microarrays ,Epidemiology ,Maternal Health ,Biochemistry ,Pregnancy ,Medicine and Health Sciences ,Medicine ,Immune Response ,Multidisciplinary ,medicine.diagnostic_test ,Obstetrics and Gynecology ,Body Fluids ,Electrophysiology ,Bioassays and Physiological Analysis ,Matrix Metalloproteinase 9 ,Cohort ,Amniocentesis ,Gestation ,Premature Birth ,Female ,Anatomy ,Research Article ,Adult ,Science ,Immunology ,Protein Array Analysis ,Preterm Birth ,Research and Analysis Methods ,Membrane Potential ,Andrology ,Signs and Symptoms ,Obstetric Labor, Premature ,Humans ,fas Receptor ,Retrospective Studies ,Inflammation ,business.industry ,Preterm Labor ,Interleukin-8 ,Case-control study ,Infant, Newborn ,Biology and Life Sciences ,Retrospective cohort study ,medicine.disease ,Amniotic Fluid ,Pregnancy Complications ,Medical Risk Factors ,Case-Control Studies ,Birth ,Women's Health ,Clinical Medicine ,business ,Premature rupture of membranes ,Biomarkers - Abstract
Objective We sought to identify novel biomarkers in the amniotic fluid (AF) related to imminent spontaneous preterm delivery (SPTD) (≤ 14 days after sampling) in women with early preterm premature rupture of membranes (PPROM), using a protein microarray. Method This was a retrospective cohort study of a total of 88 singleton pregnant women with PPROM (23+0 to 30+6 weeks) who underwent amniocentesis. A nested case-control study for biomarker discovery was conducted using pooled AF samples from controls (non-imminent delivery, n = 15) and cases (imminent SPTD, n = 15), which were analyzed using an antibody microarray. Quantitative validation of four candidate proteins was performed, using ELISA, in the total cohort (n = 88). IL-8, MMP-9, and Fas levels were additionally measured for the comparison and to examine association of SPTD with the etiologic factors of PPROM. Results Of all the proteins studied in the protein microarray, four showed significant intergroup differences. Analyses of the total cohort by ELISA confirmed the significantly elevated concentrations of AF lipocalin-2, MMP-9, and S100 A8/A9, but not of endostatin and Fas, in women who delivered within 14 days of sampling. For inflammatory proteins showing a significant association, the odds of SPTD within 14 days increased significantly with an increase in baseline AF levels of the proteins (P for trend Conclusions We identified several potential novel biomarkers (i.e., lipocalin-2, MMP-9, and S100 A8/A9) related to SPTD within 14 days of sampling, all of which are inflammation-related molecules. Furthermore, the SPTD risk increased with increasing quartiles of each of these inflammatory proteins, especially the 3rd and 4th quartile of each protein. The present findings may highlight the importance of inflammatory mechanisms and the degree of activated inflammatory response in developing SPTD in early PPROM.
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- 2020
83. Non-alcoholic fatty liver disease in the first trimester and subsequent development of gestational diabetes mellitus
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Joong Shin Park, Byoung Jae Kim, Ja Nam Koo, Gyoung Min Kim, Ig Hwan Oh, Won Kim, Sun Min Kim, Jong Kwan Jun, Soo Heon Kwak, Bo Kyung Koo, Sang Youn Kim, Chanthalakeo Vixay, Sae Kyung Joo, Sue Shin, Errol R. Norwitz, Seung Mi Lee, Chan-Wook Park, and Jeong Eun Kwon
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0301 basic medicine ,medicine.medical_specialty ,endocrine system diseases ,Endocrinology, Diabetes and Metabolism ,030209 endocrinology & metabolism ,Gastroenterology ,03 medical and health sciences ,0302 clinical medicine ,Insulin resistance ,Non-alcoholic Fatty Liver Disease ,Pregnancy ,Risk Factors ,Internal medicine ,Internal Medicine ,medicine ,Humans ,Glucose homeostasis ,Risk factor ,Prospective cohort study ,Adiponectin ,business.industry ,Fatty liver ,nutritional and metabolic diseases ,medicine.disease ,female genital diseases and pregnancy complications ,digestive system diseases ,Pregnancy Complications ,Gestational diabetes ,Diabetes, Gestational ,Pregnancy Trimester, First ,030104 developmental biology ,Female ,Insulin Resistance ,Steatosis ,business ,Biomarkers - Abstract
Although there is substantial evidence that non-alcoholic fatty liver disease (NAFLD) is associated with impaired glucose homeostasis, the clinical significance of NAFLD in pregnant women has not been well determined. This study investigates the relationship between NAFLD in the first trimester and the subsequent development of gestational diabetes mellitus (GDM). A multicentre, prospective cohort study was conducted in which singleton pregnant Korean women were assessed for NAFLD at 10–14 weeks using liver ultrasound, fatty liver index (FLI) and hepatic steatosis index (HSI). Maternal plasma adiponectin and selenoprotein P concentrations were measured. Participants were screened for GDM using the two-step approach at 24–28 weeks. Six hundred and eight women were included in the final analysis. The prevalence of NAFLD was 18.4% (112/608) and 5.9% (36/608) developed GDM. Participants who developed GDM had a higher prevalence of radiological steatosis (55.6% vs 16.1%; p
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- 2018
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84. Allogenic Pure Platelet-Rich Plasma Therapy for Rotator Cuff Disease: A Bench and Bed Study
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Seung-yeon Lee, Chris Hyunchul Jo, Kang Sup Yoon, Sue Shin, and Sohee Oh
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030222 orthopedics ,business.industry ,Physical Therapy, Sports Therapy and Rehabilitation ,030229 sport sciences ,Disease ,Bioinformatics ,medicine.disease ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,Platelet-rich plasma ,Medicine ,Orthopedics and Sports Medicine ,Rotator cuff ,Tendinopathy ,business - Abstract
Background: Although platelet-rich plasma (PRP) is a popular option for rotator cuff disease, the underlying mechanism of PRP and its clinical indications are unclear. Further, some kinds of PRP might be detrimental to patients. Allogenic PRP prepared through a standardized process and fully characterized could eliminate variations in PRP as well as uncertainties regarding its use in each patient, which could provide clues about its mechanism of action and indications for its use. Purpose: To assess the effects of pure PRP on tenocytes with or without inflammation in an in vitro study and to evaluate the safety and efficacy of a fully characterized pure PRP injection in patients with rotator cuff disease in a clinical study. Study Design: Controlled laboratory study and cohort study; Level of evidence, 3. Methods: For the in vitro study, tenocytes were enzymatically isolated and cultured from patients with rotator cuff tear and treated with or without interleukin 1β (IL-1β) and PRP. Gene expression and protein synthesis of pro- and anti-inflammatory cytokines, enzymes and their inhibitors, matrix synthesis, and cell viability were evaluated. For the clinical study, a total of 17 patients with rotator cuff disease received ultrasonography-guided subacromial PRP injection and were followed for 6 months. Pain, range of motion, muscle strength, shoulder function, and overall satisfaction in patients were compared with the results in a propensity score–matched control group who received corticosteroid (triamcinolone acetonide 40 mg). Results: PRP induced inflammation in the absence of inflammation and ameliorated inflammation in IL-1β-induced tendinopathic conditions by regulation of cytokines such as IL-1β, cyclooxygenase 2, microsomal prostaglandin E synthase 1, vasoactive intestinal peptide, and downstream matrix metalloproteinases. No general or local adverse events were noted with regard to allogenic PRP injection. Whereas steroid injection showed earlier improvement in some kinds of pain and functional scores, PRP generally showed comparable effects with steroid injection in all clinical outcomes at 6 months. Conclusion: This study showed that allogenic pure PRP had pleiotropic effects on tenocytes depending on inflammation and that it did not cause adverse events but rather decreased pain and improved shoulder function to a degree comparable with steroid injection in patients with rotator cuff disease. Clinical Relevance: Allogenic PRP could be a treatment option for rotator cuff disease.
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- 2018
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85. 487 Early prediction of pregnancy-associated hypertension : Development of novel strategies with semi-supervised learning
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Sue Shin, Ja Nam Koo, Eun Saem Choi, Ig Hwan Oh, Dokyoon Kim, Sang Youn Kim, Young Mi Jung, Sae Kyung Joo, Joong Shin Park, Byoung Jae Kim, Won Kim, Errol R. Norwitz, Sun Min Kim, Gyoung Min Kim, Chan-Wook Park, Yonghyun Nam, Seung Mi Lee, and Jacob Leiby
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medicine.medical_specialty ,Pregnancy Associated Hypertension ,business.industry ,Early prediction ,medicine ,Obstetrics and Gynecology ,Semi-supervised learning ,Intensive care medicine ,business - Published
- 2021
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86. 390 Prediction of gestational diabetes in the first trimester using machine learning-based methods
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Suhyun Hwangbo, Taesung Park, Eun Saem Choi, Errol R. Norwitz, Seung Mi Lee, Chan-Wook Park, Joong Shin Park, Young Mi Jung, Sang Youn Kim, Sun Min Kim, Sue Shin, Byoung Jae Kim, Won Kim, Gyoung Min Kim, Ig Hwan Oh, Ja Nam Koo, and Sae Kyung Joo
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Gestational diabetes ,First trimester ,medicine.medical_specialty ,business.industry ,Obstetrics ,medicine ,Obstetrics and Gynecology ,medicine.disease ,business - Published
- 2021
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87. Serum HBsAg levels during peginterferon α-2a treatment with or without thymosin α-1 in HBeAg-positive chronic hepatitis B patients
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Song, Eun Young, Shin, Yunsu, Roh, Eun Youn, Sue, Shin, Park, Myoung Hee, Kim, Bo Hyun, Kim, Won, Yoon, Jung-Hwan, Lee, Youn-Jae, Park, Sung Jae, Jung, Eun Uk, Lee, Jeong-Hoon, Myung, Sun Jung, Kim, Yoon-Jun, and Lee, Hyo-Suk
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- 2011
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88. Clinical Characteristics of Macrolide-Resistant Mycoplasma pneumoniae from Children in Jeju
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Jae Hong Choi, Kyung Sue Shin, Young Ree Kim, Yoon Joo Kim, and Keun Hwa Lee
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0301 basic medicine ,Male ,Mycoplasma pneumoniae ,030106 microbiology ,medicine.disease_cause ,Pediatrics ,Polymerase Chain Reaction ,Severity of Illness Index ,law.invention ,Microbiology ,03 medical and health sciences ,law ,23S ribosomal RNA ,Lower respiratory tract infection ,Nasopharynx ,Drug Resistance, Bacterial ,Pneumonia, Mycoplasma ,Republic of Korea ,medicine ,Prevalence ,Humans ,Child ,Pathogen ,Respiratory Tract Infections ,Polymerase chain reaction ,Respiratory tract infections ,business.industry ,23S rRNA ,General Medicine ,medicine.disease ,Antimicrobial ,respiratory tract diseases ,Anti-Bacterial Agents ,Pneumonia ,RNA, Ribosomal, 23S ,Child, Preschool ,Mutation ,Original Article ,Female ,Macrolide ,Macrolides ,business - Abstract
Mycoplasma pneumoniae is the major pathogen of community-acquired pneumonia in children. The prevalence of macrolide-resistant M. pneumoniae (MRMP) is important owing to the limited alternative therapies for children. We analyzed 111 M. pneumoniae obtained from 107 children admitted for lower respiratory tract infection at Jeju National University Hospital between 2010 and 2015. Macrolide resistance of M. pneumoniae was searched for using polymerase chain reaction (PCR) and sequencing. Of 107 clinical M. pneumoniae, 11 (10.3%) carried macrolide resistance mutations in the 23S rRNA gene. All macrolide resistance mutations were A2063G transitions. We found an acquired A2063G mutation of M. pneumoniae from a patient during macrolide treatment. Patients' characteristics and clinical severity did not differ between those with MRMP and macrolide-sensitive M. pneumoniae, with the exception of frequent pleural effusion in the MRMP group. The prevalence of MRMP (10.3%) in Jeju Island was relatively lower than those of surrounding countries in East Asia. Previous antimicrobial usage and timing of diagnostic test should be considered when determining of macrolide resistance of M. pneumoniae., Graphical Abstract
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- 2017
89. Long-Term Quality Control Program Plan for Cord Blood Banks in Korea: A Pilot Study for Cryopreservation Stability
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Jong Hyun Yoon, Sohee Oh, Soo Hyun Seo, Sue Shin, Byoung Jae Kim, Eun Youn Roh, and Eun Young Song
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Quality Control ,Internal quality control ,medicine.medical_specialty ,Time Factors ,Cell Survival ,Clinical Biochemistry ,Antigens, CD34 ,Cell Count ,Pilot Projects ,030204 cardiovascular system & hematology ,Cryopreservation ,Andrology ,Umbilical cord blood ,03 medical and health sciences ,0302 clinical medicine ,Nucleated cell ,Long period ,Republic of Korea ,Humans ,Medicine ,Viability assay ,Transfusion Medicine ,business.industry ,Biochemistry (medical) ,Quality control ,General Medicine ,Fetal Blood ,Capillaries ,Internal quality ,Surgery ,Transplantation ,Cord blood ,Blood Banks ,Original Article ,business ,030215 immunology - Abstract
Background Maintaining the quality of cryopreserved cord blood is crucial. In this pilot study, we describe the results of the internal quality control program for a cord blood bank thus far. Methods Donated cord blood units unsuitable for transplantation were selected for internal quality control once a month. One unit of cord blood, aliquoted into 21 capillaries, was cryopreserved and thawed annually to analyze the total nucleated cell count, CD34⁺ cell count, cell viability test, and colony-forming units assay. Results No significant differences in the variables (total nucleated cell count, cell viability, CD34⁺ cell count) were observed between samples cryopreserved for one and two years. Upon comparing the variables before cryopreservation and post thawing with the capillaries of one year of storage, cell viability and CD34⁺ cell counts decreased significantly. The use of cord blood samples in capillaries, which can be easily stored for a long period, was similar to the methods used for testing segments attached to the cord blood unit. Conclusions The results of this study may be useful for determining the period during which the quality of cryopreserved cord blood units used for transplantation is maintained.
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- 2017
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90. ALLOGENEIC PLATELET-RICH PLASMA FOR ROTATOR CUFF REPAIR
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Ji Sun Shin, Sue Shin, Chris Hyunchul Jo, and Seung-Yeon Lee
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Rotator cuff ,Orthopedic surgery ,Gynecology ,030222 orthopedics ,medicine.medical_specialty ,business.industry ,Rehabilitation ,Physical Therapy, Sports Therapy and Rehabilitation ,Original Articles ,030229 sport sciences ,Tendon injuries ,03 medical and health sciences ,0302 clinical medicine ,Platelet-rich plasma ,Medicine ,Orthopedics and Sports Medicine ,Growth factors ,business ,RD701-811 - Abstract
Objetivo: Investigar a seguranca e eficacia do plasma rico em plaquetas alogenico (PRP) no reparo do manguito rotador. Metodos: Foram incluidos dezessete pacientes com ruptura da espessura total do manguito rotador. Dez pacientes foram submetidos a reparo artroscopico do manguito rotador com PRP alogenico e sete pacientes com PRP autologo. Tres geis de PRP de 3ml cada foram aplicados entre a extremidade lesionada e a tuberosidade maior. Os resultados clinicos foram avaliados no pre-operatorio e no minimo 2 anos apos a cirurgia. Os resultados estruturais foram avaliados com a presenca de nova ruptura e a alteracao da area em corte transversal (ACT) do supra-espinhal. Resultados: O PRP alogenico nao causou quaisquer eventos adversos durante o periodo de acompanhamento. Nao houve diferenca significativa nas medidas de resultados clinicos entre os dois grupos (todos os valores p> 0,05). A taxa de nova ruptura foi de 33,3% e 25,0% no grupo alogenico e grupo autologo, respectivamente (p = 0,764). A alteracao da ACT entre o pos-operatorio de um ano e imediatamente no pos-operatorio tambem nao foi significativamente diferente entre os dois grupos (p = 0,373) . Conclusao: O PRP alogenico administrado por via artroscopica no manguito rotador nao causou quaisquer complicacoes locais ou gerais e sua eficacia e comparavel ao PRP autologo no que diz respeito aos resultados clinicos e estruturais. Nivel de Evidencia III, Estudo Retrospectivo Comparativo.
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- 2017
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91. Excluding Anti-cytomegalovirus Immunoglobulin M-Positive Cord Blood Units Has a Minimal Impact on the Korean Public Cord Blood Bank Inventory
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Eui Chong Kim, Sohee Oh, Eun Young Song, Jong Hyun Yoon, Sue Shin, and Eun Youn Roh
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0301 basic medicine ,Biomedical Engineering ,Congenital cytomegalovirus infection ,lcsh:Medicine ,Cytomegalovirus ,Antigens, CD34 ,Antibodies, Viral ,Article ,Immunoglobulin G ,03 medical and health sciences ,0302 clinical medicine ,Pregnancy ,Republic of Korea ,medicine ,Humans ,Seroprevalence ,Transplantation ,Communicable disease ,biology ,business.industry ,lcsh:R ,Cell Biology ,Fetal Blood ,medicine.disease ,030104 developmental biology ,Immunoglobulin M ,Cord blood ,Immunology ,Linear Models ,biology.protein ,Blood Banks ,Female ,Antibody ,business ,030215 immunology - Abstract
Cord blood units (CBUs) for transplantation should be free of communicable disease and must contain a specific amount of total nucleated cells and CD34+ cells. Although posttransplantation cytomegalovirus (CMV) infections are from latent infection in patients, ensuring CMV-free CBUs by performing CMV-specific IgM and nucleic acid amplification testing (NAT) is one of the mandatory procedures for the safety of CBUs. However, the exclusion policies (based on these test results) vary among nations and institutions. We tested 28,000 processed CBUs between May 2006 and June 2014. The cord blood leukocytes from CMV IgM-positive samples were then subjected to NAT. The total nucleated cell and CD34+ cell counts were measured for each CBU, and the results were compared to the CMV IgM and IgG results. The seroprevalence of CMV among pregnant women was 98.1% (18,459/18,818) for IgG and 1.7% (441/25,293) for IgM. The concentration and the total number of CD34+ cells were significantly higher in CBUs from IgM-negative mothers compared to those from IgM-positive mothers (72.4/μl vs. 57.2/μl, respectively, p < 0.0001; 1.45 × 106/unit vs. 1.15 × 106/unit, respectively, p < 0.0001). Among CBUs with positive CMV IgM in their mothers' plasma or cord blood plasma, only 0.58% of the samples (3/517) had a positive NAT. The number of excluded CBUs from inventory due to positive CMV IgM in the cord blood was 54 of 18,326 (0.3%). For inventory purposes, it is appropriate to remove CBUs with positive cord blood CMV IgM findings irrespective of the NAT status as well as positive maternal CMV IgM in South Korea.
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- 2017
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92. Effects of Interleukin-4 and Interleukin-12b Gene Polymorphisms on Hepatitis B Virus Vaccination
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Hyunwoong Park, Jong Hyun Yoon, Eun Young Song, Soo Hyun Seo, Sohee Oh, Ju Young Chang, Sue Shin, and Eun Youn Roh
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Male ,0301 basic medicine ,Hepatitis B virus ,Time Factors ,Genotype ,Vaccine response ,Specialties of internal medicine ,Korean ,Single-nucleotide polymorphism ,Hbv vaccination ,medicine.disease_cause ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,0302 clinical medicine ,Gene Frequency ,Republic of Korea ,Humans ,Medicine ,Hepatitis B Vaccines ,Hepatitis Antibodies ,Gene ,Immunization Schedule ,Interleukin 4 ,Hepatitis B Surface Antigens ,Hepatology ,Interleukin-12 Subunit p40 ,business.industry ,Vaccination ,Infant ,Interleukin-12B gene polymorphism ,Interleukin ,General Medicine ,Hepatitis B ,Interleukin-4 gene polymorphism ,Phenotype ,Treatment Outcome ,030104 developmental biology ,RC581-951 ,Pharmacogenetics ,Immunology ,Female ,030211 gastroenterology & hepatology ,Interleukin-4 ,business ,Biomarkers - Abstract
Approximately 10% of individuals do not respond to hepatitis B virus (HBV) vaccination, i.e. non-responders (NRs). We aimed to investigate the association of interleukin (IL)-4 and IL-12B gene polymorphisms with responsiveness to the HBV vaccine in Korean infants. Among 300 healthy infants (9-12 month), SNPs for the IL-4 gene (rs2243250, rs2070874, and rs2227284) and for the IL-12B gene (rs3213094 and rs17860508) were compared between subgroups in terms of the response to HBV vaccination. The percentages of NRs (< 10 mIU/mL), low-titer responders (LRs, 10-100 mIU/mL), and high-titer responders (HRs, ≥ 100 mIU/mL) were 20.3%, 37.7% and 42.0%, respectively. No SNPs differed in frequency between NRs and responders or between LRs and HRs. We divided the subjects into two groups according to the time interval from the 3rd dose of HBV vaccination to Ab quantification: > 6 months from the 3rd dose (n = 87) and ≤ 6 months from the 3rd dose (n = 213). In the ≤ 6 month subjects, rs2243250C and rs2227284G were significantly frequent in the lower-titer individuals (NRs + LR) than HRs (40.1 vs. 25.9%, p = 0.014 and 45.1 vs. 33.0%, p = 0.018, respectively), and the rs2243250C and rs2227284G frequencies were significantly different among the three subgroups (13.2 vs. 26.9 vs. 25.9%, p = 0.040 and 15.5 vs. 29.6 vs. 33.0%, p = 0.038, respectively). In conclusion, those results suggest that IL-4 gene polymorphisms may play a role in the response to the HBV vaccine in Korean infants.
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- 2017
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93. Maternal lipid profiles vs. fetal growth and cord blood hematopoietic cells: weak associations in healthy Korean newborn-mother pairs.
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un Young SONG, Jong Hgun YOON, Sue SHIN, Ju Young CHANG, Ki-Tae HWANG, and Eun Youn ROH
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- 2022
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94. Prevalence and correlated factors of sexually transmitted infections among women attending a Korean sexual assault center
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Hyunwoong Park, Sue Shin, Eun Youn Roh, Jong Hyun Yoon, Jae Hyeon Park, and Nam Hee Kim
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Adult ,medicine.medical_specialty ,Population ,Sexually Transmitted Diseases ,Poison control ,Chlamydia trachomatis ,Mycoplasma hominis ,urologic and male genital diseases ,medicine.disease_cause ,Ambulatory Care Facilities ,Pathology and Forensic Medicine ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Age Distribution ,Republic of Korea ,medicine ,Prevalence ,Humans ,030216 legal & forensic medicine ,030212 general & internal medicine ,education ,Pyuria ,Crime Victims ,Retrospective Studies ,education.field_of_study ,biology ,Obstetrics ,business.industry ,Sex Offenses ,virus diseases ,General Medicine ,biology.organism_classification ,female genital diseases and pregnancy complications ,Trichomonas vaginalis ,Female ,medicine.symptom ,Mycoplasma genitalium ,business ,Law ,Ureaplasma urealyticum - Abstract
Long-term observation of the prevalence of sexually transmitted infections (STIs) is unclear in sexual assault victims (SAVs).We aimed to identify the trends in the prevalence of STIs in SAVs and factors correlated with STIs. Between January 2010 and May 2019, a retrospective observational study was conducted in a regional sexual assault center in Seoul. Data were collected from sexual assault questionnaires and laboratory results. PCR for STIs, including Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Mycoplasma genitalium (MG), Mycoplasma hominis (MH), Ureaplasma urealyticum (UU), Trichomonas vaginalis (TV) and cytomegalovirus (CMV), was performed via vaginal swab. Associations between STIs and covariates were examined by logistic regression. A total of 1843 SAVs were included, and 60.2% were positive for at least one STI. UU was the dominant agent (32.6%), followed by MH (27.7%) and CT (17.4%). The annual positivity rate ofMH showed a significant decreasing trend (tau = -0.600, P value = 0.020). The highest positivity rate of STI by age group was 68.0% (282/415) in the 15-19 years of age group. Age (OR = 0.951, 95% confidence interval [CI] = 0.934-0.968) and pyuria (OR = 1.898, 95% CI = 1.543-2.329) were associated with clinically important STIs (NG, CT and TV). The prevalence of STIs in SAVs was consistently high over 10 years, and most STIs were more prevalent in SAVs than in the general population. Presumptive treatment is still needed, especially for young SAVs with pyuria.
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- 2019
95. Association of Antibody-Secreting Cells With Allograft Rejection After Renal Transplantation
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Jaeseok Yang, Jongwon Ha, Sue Shin, Hyo-Suk Lee, Kyoung Un Park, Eun-Young Song, Minjeong Nam, and Eun Youn Roh
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Adult ,Graft Rejection ,Male ,medicine.medical_specialty ,Enzyme-Linked Immunospot Assay ,Urology ,chemical and pharmacologic phenomena ,Kidney ,Peripheral blood mononuclear cell ,Antibodies ,Flow cytometry ,Young Adult ,Risk Factors ,medicine ,Humans ,Transplantation, Homologous ,Antibody-Producing Cells ,Sensitization ,Transplantation ,CD40 ,medicine.diagnostic_test ,biology ,business.industry ,ELISPOT ,Autologous lymphocyte ,Middle Aged ,Allografts ,Flow Cytometry ,Kidney Transplantation ,medicine.anatomical_structure ,biology.protein ,Surgery ,Female ,Antibody ,business - Abstract
Background Donor-specific antibodies (DSA) are measured at the time of transplantation to predict renal allograft outcome, but pretransplantation DSA are sometimes not adequate to predict antibody-mediated rejection (AMR). We previously developed a flow cytometric assay that could measure the number of antibody-secreting cells (ASCs) instead of DSA. Here, we evaluated the performance of the flow cytometric ASC assay in predicting renal allograft rejection and compared it with that of the enzyme-linked immunospot (ELISpot) assay. Methods We enrolled 25 patients who received renal transplantation between May 2017 and August 2017 at Seoul National University Hospital. Mononuclear cells separated from patient peripheral blood obtained on pretransplantation day 1 were incubated with CpG 2006, human CD40L, interleukin-21, and donor or autologous lymphocyte lysates for 6 days. Flow cytometry and ELISpot assay (Mabtech) were performed to measure the ASCs and their association with graft rejection. Results The number of donor-reactive ASCs, as measured by flow cytometry, was higher in the rejection group than in the nonrejection group (mean ± standard deviation [SD], 3688.9 ± 3875.3 vs 257.9 ± 297.3, P = .014), and no significant difference was observed in the ELISpot assay. Multiple linear regression analysis showed that the number of donor-reactive ASCs measured by flow cytometry was independently and negatively associated with the number of rejection-free days (P = .008, partial R2 = 0.368, adjusted R2 = 0.496). Conclusion After renal transplantation, an increased number of donor-reactive ASCs, as measured by flow cytometry, was associated with allograft rejection. This may be useful to predict renal allograft rejection by measuring the sensitization status of patients who are awaiting renal transplantation.
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- 2019
96. Removal of Non-specific Reactions of LABScreen Single Antigen Class II Assay by Fetal Bovine Serum Treatment
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Kiwook, Jung, Minjeong, Nam, Yun Ji, Hong, Eun Youn, Roh, Sue, Shin, Kyoung Un, Park, and Eun Young, Song
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Adult ,Immunoassay ,Male ,Serum ,Fetus ,Case-Control Studies ,Histocompatibility Antigens Class II ,Animals ,Humans ,Cattle ,Female - Abstract
Recently, the removal of false reaction from beads 10 (
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- 2019
97. Correction: Nonalcoholic fatty liver disease is a risk factor for large-for-gestational-age birthweight
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Ja Nam Koo, Sue Shin, Errol R. Norwitz, Byoung Jae Kim, Joong Shin Park, Gyoung Min Kim, Won Kim, Jong Kwan Jun, Sun Min Kim, Chan-Wook Park, Sae Kyung Joo, Soo Heon Kwak, Sang Youn Kim, Seung Mi Lee, Chanthalakeo Vixay, and Ig Hwan Oh
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Adult ,medicine.medical_specialty ,Science ,Gestational Age ,Weight Gain ,Gastroenterology ,Infant, Newborn, Diseases ,Body Mass Index ,Young Adult ,Fetus ,Non-alcoholic Fatty Liver Disease ,Pregnancy ,Risk Factors ,Internal medicine ,Nonalcoholic fatty liver disease ,medicine ,Birth Weight ,Humans ,Risk factor ,Multidisciplinary ,business.industry ,Fatty liver ,Infant, Newborn ,Gestational age ,Correction ,medicine.disease ,Pregnancy Complications ,Diabetes, Gestational ,Medicine ,Female ,Insulin Resistance ,business - Abstract
Nonalcoholic fatty liver disease (NAFLD) is a well-recognized hepatic manifestation of metabolic disease in adults and has been associated with the development of gestational diabetes (GDM). Hepatic insulin resistance can result in increased release of glucose (from gluconeogenesis) and free fatty acids (due to enhanced lipolysis), which can lead in turn to fetal overgrowth. However, the relationship between maternal metabolic factors (such as circulating levels of triglycerides, free fatty acids [FFA], or adipokines) and excessive fetal birthweight in NAFLD has not been carefully examined. In this study, we evaluated the relationship between NAFLD and the subsequent risk of large-for-gestational-age (LGA) birthweight.Singleton nondiabetic pregnant women were evaluated for the presence of fatty liver at 10-14 weeks of gestation by abdominal ultrasound. The degree of fatty liver was classified as Grade 0-3 steatosis. At the time of liver ultrasound, maternal blood was taken after fasting and measured for adiponectin and FFA. LGA was defined as birthweight90th percentile for gestational age.A total of 623 women were included in the analysis. The frequency of LGA was 10.9% (68/623), and the frequency of NAFLD was 18.9%. The risk of LGA increased significantly in patients with Grade 2-3 steatosis in the first trimester. The relationship between Grade 2-3 steatosis and LGA remained significant after adjustment for maternal age, pre-pregnancy BMI, GDM, and maternal serum triglyceride levels. The concentration of maternal blood adiponectin at 10-14 weeks was significantly lower in cases with LGA than non-LGA, but the maternal blood FFA concentrations were not different between the groups.The presence of Grade 2-3 steatosis on ultrasound in early pregnancy was associated with the increased risk of delivering an LGA infant, even after adjustment for multiple confounding factors including GDM. Adiponectin may be the linking biomarker between NAFLD and LGA.
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- 2019
98. Maternal lipid profiles vs. fetal growth and cord blood hematopoietic cells: weak associations in healthy Korean newborn-mother pairs
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Eun Young SONG, Jong Hgun YOON, Sue SHIN, Ju Young CHANG, Ki-Tae HWANG, and Eun Youn ROH
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Fetal Development ,Pregnancy ,Pediatrics, Perinatology and Child Health ,Republic of Korea ,Infant, Newborn ,Humans ,Antigens, CD34 ,Female ,Fetal Blood ,Triglycerides - Abstract
We aimed to define the maternal lipid profiles that are associated with fetal growth and cord blood (CB) hematopoietic cells in healthy Korean full-term newborns.A total of 608 fetal-maternal pairs were enrolled; mothers voluntarily donated CB with informed consent. We analyzed birth weight (BW) as a marker of fetal growth, and we examined total nucleated cells (TNCs) and CD34Maternal triglycerides (TG) showed a significant positive association with BW and CD34We were able to investigate the association of maternal lipid profiles with BW and CB HPCs in healthy Korean newborn-mother pairs in this study. Both BW and the HPC contents showed independent associations with maternal TG and LDL, although the effect of maternal lipid levels on fetal growth and HPCs was not strong in the normal healthy population. Because maternal lipid levels were assessed once in the healthy fetal-maternal pairs, we could not investigate those associations across pregnancy.
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- 2019
99. A Comparison of Predictive Performances between Old versus New Criteria in a Risk-Based Screening Strategy for Gestational Diabetes Mellitus
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Seung Mi Lee, Souphaphone Louangsenlath, Sun Min Kim, Joong Shin Park, Sue Shin, Byoung Jae Kim, Sohee Oh, Sae Kyung Joo, Ig Hwan Oh, Subeen Hong, Won Kim, Jong Kwan Jun, Chan-Wook Park, Ja Nam Koo, Soo Heon Kwak, and Errol R. Norwitz
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medicine.medical_specialty ,endocrine system diseases ,Endocrinology, Diabetes and Metabolism ,lcsh:Diseases of the endocrine glands. Clinical endocrinology ,Pregnancy ,Secondary analysis ,Diabetes mellitus ,Nonalcoholic fatty liver disease ,Medicine ,Humans ,Mass Screening ,Prospective Studies ,Prospective cohort study ,Pregnancy, high-risk ,lcsh:RC648-665 ,Diagnostic screening programs ,business.industry ,Obstetrics ,Fasting ,Glucose Tolerance Test ,medicine.disease ,female genital diseases and pregnancy complications ,Gestational diabetes ,Diabetes, Gestational ,Gestation ,Female ,Original Article ,Metabolic Risk/Epidemiology ,business - Abstract
Background: The definition of the high-risk group for gestational diabetes mellitus (GDM) defined by the American College of Obstetricians and Gynecologists was changed from the criteria composed of five historic/demographic factors (old criteria) to the criteria consisting of 11 factors (new criteria) in 2017. To compare the predictive performances between these two sets of criteria. Methods: This is a secondary analysis of a large prospective cohort study of non-diabetic Korean women with singleton pregnan cies designed to examine the risk of GDM in women with nonalcoholic fatty liver disease. Maternal fasting blood was taken at 10 to 14 weeks of gestation and measured for glucose and lipid parameters. GDM was diagnosed by the two-step approach. Results: Among 820 women, 42 (5.1%) were diagnosed with GDM. Using the old criteria, 29.8% (n=244) of women would have been identified as high risk versus 16.0% (n=131) using the new criteria. Of the 42 women who developed GDM, 45.2% (n=19) would have been mislabeled as not high risk by the old criteria versus 50.0% (n=21) using the new criteria (1-sensitivity, 45.2% vs. 50.0%, P>0.05). Among the 778 patients who did not develop GDM, 28.4% (n=221) would have been identified as high risk us ing the old criteria versus 14.1% (n=110) using the new criteria (1-specificity, 28.4% vs. 14.1%, P
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- 2019
100. Maternal lipid profiles vs. fetal growth and cord blood hematopoietic cells: weak associations in healthy Korean newborn-mother pairs
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Sue Shin, Jong H Yoon, Eun Youn Roh, Eun Young Song, Ju Y Chang, and Ki-Tae Hwang
- Subjects
Pregnancy ,business.industry ,Birth weight ,Cell ,CD34 ,medicine.disease ,Andrology ,Haematopoiesis ,medicine.anatomical_structure ,Cord blood ,Pediatrics, Perinatology and Child Health ,Medicine ,business ,Beta (finance) ,Lipoprotein - Abstract
Background We aimed to define the maternal lipid profiles that are associated with fetal growth and cord blood (CB) hematopoietic cells in healthy Korean full-term newborns. Methods A total of 608 fetal-maternal pairs were enrolled; mothers voluntarily donated CB with informed consent. We analyzed birth weight (BW) as a marker of fetal growth, and we examined total nucleated cells (TNCs) and CD34+ cell concentrations of CB as markers of hematopoietic progenitor cell (HPC) contents. We also analyzed maternal lipid levels and investigated their associations with BW, TNCs and CD34+ cells. Results Maternal triglycerides (TG) showed a significant positive association with BW and CD34+ cells, and low-density lipoprotein (LDL) showed a negative association with BW and CD34+ cells. Though not statistically significant, higher maternal TG showed a tendency toward higher levels of TNCs. Maternal TG was independently and positively correlated with BW, and maternal LDL was independently and negatively correlated with CD34+ cells, although the impacts were not as strong, as indicated by small beta coefficients (0.157 and -0.226, respectively). Conclusions We were able to investigate the association of maternal lipid profiles with BW and CB HPCs in healthy Korean newborn-mother pairs in this study. Both BW and the HPC contents showed independent associations with maternal TG and LDL, although the effect of maternal lipid levels on fetal growth and HPCs was not strong in the normal healthy population. Because maternal lipid levels were assessed once in the healthy fetal-maternal pairs, we could not investigate those associations across pregnancy.
- Published
- 2019
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