51. Refinement of herpesvirus B-capsid structure on parallel supercomputers.
- Author
-
Zhou ZH, Chiu W, Haskell K, Spears H Jr, Jakana J, Rixon FJ, and Scott LR
- Subjects
- Computer Simulation, Freezing, Microscopy, Electron, Models, Molecular, Simplexvirus ultrastructure, Capsid chemistry, Capsid ultrastructure, Protein Conformation, Simplexvirus chemistry
- Abstract
Electron cryomicroscopy and icosahedral reconstruction are used to obtain the three-dimensional structure of the 1250-A-diameter herpesvirus B-capsid. The centers and orientations of particles in focal pairs of 400-kV, spot-scan micrographs are determined and iteratively refined by common-lines-based local and global refinement procedures. We describe the rationale behind choosing shared-memory multiprocessor computers for executing the global refinement, which is the most computationally intensive step in the reconstruction procedure. This refinement has been implemented on three different shared-memory supercomputers. The speedup and efficiency are evaluated by using test data sets with different numbers of particles and processors. Using this parallel refinement program, we refine the herpesvirus B-capsid from 355-particle images to 13-A resolution. The map shows new structural features and interactions of the protein subunits in the three distinct morphological units: penton, hexon, and triplex of this T = 16 icosahedral particle.
- Published
- 1998
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