51. Short-Term High-Salt Diet Increases Corin Level to Regulate the Salt-Water Balance in Humans and Rodents.
- Author
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Zhang, Jiao, Yin, Yanjun, Chen, Lili, Chu, Chao, Wang, Yang, Lv, Yongbo, He, Ming, Martin, Marcy, Huang, Po-Hsun, Mu, Jian-Jun, Shyy, John Y-J, and Yuan, Zu-Yi
- Subjects
Kidney Disease ,Cardiovascular ,Nutrition ,Hypertension ,Adaptation ,Physiological ,Adult ,Albuminuria ,Animals ,Aquaporin 2 ,Blood Pressure ,Diet ,Sodium-Restricted ,Epithelial Sodium Channels ,Female ,Humans ,Kidney ,Male ,Middle Aged ,Natriuresis ,Potassium ,Dietary ,Proprotein Convertase 9 ,Rats ,Sprague-Dawley ,Serine Endopeptidases ,Sodium Chloride ,Dietary ,Time Factors ,Up-Regulation ,Water-Electrolyte Balance ,blood pressure ,corin ,high-salt diet ,hypertension ,potassium ,PCSK6 ,Clinical Sciences ,Cardiovascular System & Hematology - Abstract
BackgroundDietary sodium and potassium affect the fluctuation in blood pressure (BP) and renal function. Corin, with its enzymatic activity to convert pro-atrial natriuretic peptide (pro-ANP) to biologically active ANP, regulates BP, cardiac, and renal functions. We investigated whether corin expression responds to a high-salt (HS) diet to regulate salt and water balance.MethodsForty-two volunteers followed 3 sequential diets for 7 days each: a low-salt (LS) diet (3.0 g/day NaCl), a HS diet (18.0 g/day NaCl), followed by an HS diet with K+ supplementation (HS + K+) (18.0 g/day NaCl and 4.5 g/day KCl).ResultsCorin level was higher with the HS diet than the LS and HS + K+ diets and was positively correlated with systolic BP (SBP) and 24-hour urinary Na+ and microalbumin (U-mALB) excretion. In rodents, serum and renal levels of corin were transiently increased with the HS diet and were decreased if the HS diet was continued for up to 7 days. HS loading increased SBP, 24-hour urinary Na+, U-mALB excretion, and the expression of proprotein convertase subtilisin/kexin-6 (PCSK6), a corin activator. Knockdown of PCSK6 or corin in high salt-treated M1-cortical collecting duct (M1-CCD) cells increased the expression of aquaporin 2 (AQP2) and β-epithelial Na+ channel (β-ENaC).ConclusionsShort-term HS may induce the PCSK6-corin-ANP-AQP2/β-ENaC pathway in the kidney. Enhanced serum corin level in humans and rodents is positively correlated with HS-induced SBP and 24-hour urinary Na+ and U-mALB excretion, which suggests that corin is involved in the salt-water balance in response to HS intake.Clinical trials registrationPublic Trials Registry Number NCT02915315.
- Published
- 2018