51. Electrochemical and spectroscopic study on the interaction between isoprenaline and DNA using multivariate curve resolution-alternating least squares
- Author
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Serge Kokot, Min Wei, and Yongnian Ni
- Subjects
Absorption spectroscopy ,Analytical chemistry ,Electrochemistry ,Biochemistry ,Chemometrics ,Structural Biology ,Spectrophotometry ,medicine ,Animals ,Least-Squares Analysis ,Electrodes ,Molecular Biology ,Voltammetry ,060100 BIOCHEMISTRY AND CELL BIOLOGY ,medicine.diagnostic_test ,Chemistry ,Spectrum Analysis ,Isoproterenol ,Hyperchromicity ,DNA ,Electrochemical Techniques ,General Medicine ,Hydrogen-Ion Concentration ,Binding constant ,Carbon ,Kinetics ,Isoprenaline, DNA, Spectrophotometry, Voltammetry, Multivariate curve resolution-alternating least squares ,Multivariate Analysis ,Cattle ,Glass ,Cyclic voltammetry ,Oxidation-Reduction - Abstract
Interaction of isoprenaline (ISO) with calf-thymus DNA was studied by spectroscopic and electrochemical methods. The behavior of ISO was investigated at a glassy carbon electrode (GCE) by cyclic voltammetry (CV) and differential pulse stripping voltammetry (DPSV); ISO was oxidized and an irreversible oxidation peak was observed. The binding constant K and the stoichiometric coefficient m of ISO with DNA were evaluated. Also, with the addition of DNA, hyperchromicity of the UV-vis absorption spectra of ISO was noted, while the fluorescence intensity decreased significantly. Multivariate curve resolution-alternating least squares (MCR-ALS) chemometrics method was applied to resolve the combined spectroscopic data matrix, which was obtained by the UV-vis and fluorescence methods. Pure spectra of ISO, DNA and ISO-DNA complex, and their concentration profiles were then successfully obtained. The results indicated that the ISO molecule intercalated into the base-pairs of DNA, and the complex of ISO-DNA was formed.
- Published
- 2011