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583 results on '"Sellergren, Börje"'

61. Sialic acid as a biomarker studied in breast cancer cells using fluorescent molecularly imprinted polymers, MIPs

Author

El-Schich, Zahra, primary, Zhang, Yuecheng, additional, Göransson, Tommy, additional, Dizeyi, Nishtman, additional, Persson, Jenny, additional, Johansson, Emil, additional, Caraballo, Remi, additional, Elofsson, Mikael, additional, Shinde, Sudhirkumar, additional, Sellergren, Börje, additional, and Wingren, Anette Gjörloff, additional

Published
2020
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84. Hierarchically templated beads with tailored pore structure for phosphopeptide capture and phosphoproteomics

Author

Wierzbicka, Celina, Torsetnes, Silje B., Jensen, Ole N., Shinde, Sudhirkumar, and Sellergren, Börje

Subjects
Chemistry, Multidisciplinary, Naturvetenskap, Natural Sciences
Abstract

Two templating approaches to produce imprinted phosphotyrosine capture beads with a controllable pore structure are reported and compared with respect to their ability to enrich phosphopeptides from a tryptic peptide mixture. The beads were prepared by the polymerization of urea-based host monomers and crosslinkers inside the pores of macroporous silica beads with both free and immobilized template. In the final step the silica was removed by fluoride etching resulting in mesoporous polymer replicas with narrow pore size distributions, pore diameters ≈ 10 nm and surface area > 260 m2 g-1. The beads displayed pronounced phosphotyrosine affinity and selectivity in binding tests using model peptides in acetonitrile rich solutions with a performance surpassing solution polymerized bulk imprinted materials. Tests of the beads for the enrichment of phosphopeptides from tryptic digests of twelve proteins revealed both pY/pS and pY/Y selectivity. This was reflected in a nearly 6-fold increase in the enrichment factor of a 23-mer pY-peptide and pY/pS normalized intensity ratios up to 1.5, when comparing the template mesoporous beads with the bulk materials.

Published
2017

85. Molecularly Imprinted Polymer of Colocynthin, An Effective Tool for Quality Control of Citrullus colocynthis Extracts

Author

Ramezani Farid, Shams ardekani Mohammad Reza, Shekarchi Maryam, Rastegar Hossein, Khanavi Mahnaz, Eftekhari Mahdieh, and Sellergren Börje

Subjects
chemistry.chemical_classification, Quality Control, Sorbent, Chromatography, Chemistry, Plant Extracts, Polymers, Solid Phase Extraction, Molecularly imprinted polymer, Polymer, Porosimetry, Dosage form, Molecular Imprinting, Adsorption, Citrullus colocynthis, Drug Discovery, Spectroscopy, Fourier Transform Infrared, Thermogravimetry, Microscopy, Electron, Scanning, Plant Oils, Solid phase extraction, Chromatography, High Pressure Liquid
Abstract

Background: Different parts of Colocynth, Citrullus colocynthis (L.) Schrad., are used in traditional phytotherapy and homeopathy. Objective: In our new approach, a molecularly imprinted polymer was synthesized to absorb colocynthin, the major plant marker, and its capability was evaluated using HPLC-UV. Method: A new method was considered to achieve optimal conditions. FT-infrared, N2 adsorption porosimetry, fluorescent and scanning electron microscopy and thermo gravimetric profile of the polymers were studied. The imprinted polymer was applied as molecularly imprinted solid phase extraction sorbent to enrich colocynthin from colocynth oil extract, a traditional medicine dosage form. Results: The imprinted polymer showed high capacity and affinity toward colocynthin. Physical assessments demonstrated no major differences between imprinted and non-imprinted polymers. The imprinted polymer was able to absorb colocynthin more efficiently than non-imprinted and control simple solvent extraction from the real sample. Conclusion: In conclusion, this polymer is capable of being applied as a promising adsorbent for analysis of colocynth traditional medicine products.

Published
2016

89. Phosphotyrosine Biased Enrichment of Tryptic Peptides from Cancer Cells by Combining pY-MIP and TiO2Affinity Resins

Author

Bllaci, Loreta, Torsetnes, Silje B., Wierzbicka, Celina, Shinde, Sudhirkumar, Sellergren, Börje, Rogowska-Wrzesinska, Adelina, and Jensen, Ole N.

Abstract

Protein phosphorylation at distinct tyrosine residues (pY) is essential for fast, specific, and accurate signal transduction in cells. Enrichment of pY-containing peptides derived from phosphoproteins is commonly facilitated by use of immobilized anti-pY antibodies prior to phosphoproteomics analysis by mass spectrometry. We here report on an alternative approach for pY-peptide enrichment using inexpensive pY-imprinted polymer (pY-MIP). We assessed by mass spectrometry the performance of pY-MIP for enrichment and sequencing of phosphopeptides obtained by tryptic digestion of protein extracts from HeLa cells. The combination of pY-MIP- and TiO2-based phosphopeptide enrichment provided more than 90% selectivity for phosphopeptides. Mass spectrometry signal intensities were enhanced for most pY-phosphopeptides (approximately 70%) when using the pY-MIP-TiO2combination as compared to TiO2alone. pY constituted up to 8% of the pY-MIP-TiO2-enriched phosphopeptide fractions. The pY-MIP-TiO2and the TiO2protocols yielded comparable numbers of distinct phosphopeptides, 1693 and 1842, respectively, from microgram levels of peptide samples. Detailed analysis of physicochemical properties of pY-MIP-TiO2-enriched phosphopeptides demonstrated that this protocol retrieved phosphopeptides that tend to be smaller (<24 residues), less acidic, and almost exclusively monophosphorylated, as compared to TiO2alone. These unique properties render the pY-MIP-based phosphopeptide enrichment technique an attractive alternative for applications in phosphoproteomics research.

Published
2024
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