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51. Планирование деятельности бюджетных учреждений с учетом рейтинга

Author

Сазонова, И. В., Sazonova, I. V., Сазонова, И. В., and Sazonova, I. V.

Abstract

В изменившихся условиях финансирования бюджетным учреждениям необходимо планировать экономическое развитие и финансово-хозяйственную деятельность. В статье предлагается проводить сравнительный рейтинговый анализ на основе планируемых относительных показателей структурных подразделений учреждений., In the changed terms of financing it is necessary for budgetary establishments to plan economic development and financial and economic activity. In article it is offered to carry out the comparative rating analysis on the basis of planned relative indicators of structural divisions of establishments.

Published
2012

52. Features of constructive solutions of high-rise buildings

Author

Sazonova, I. R. and Sazonova, I. R.

Abstract

The issues of the design of high-rise buildings, both on the action of permanent and temporary loads, and on actions caused by emergency situations, which can lead to an emergency collapse of one of the main supporting structures, are considered. The conclusions are made and recommendations on constructive decisions which allow to localize consequences of an accident are given., Рассмотрены вопросы особенностей проектирования высотных зданий как на действие постоянных и временных нагрузок, так и на действия, вызванные чрезвычайными ситуациями, которые могут привести к аварийному обрушения одной из основных несущих конструкций. Сделаны выводы и даны рекомендации по конструктивным решениям, которые позволяют локализовать последствия аварии., Розглянуто питання особливостей проектування висотних будівель як на дію постійних і тимчасових навантажень, так і на дії, викликані надзвичайними ситуаціями, які можуть привести до аварійного обвалення однієї з основних несучих конструкцій. Зроблено висновки і надано рекомендації щодо конструктивних рішень, якi дають змогу локалізувати наслідки аварії.

Published
2009

54. Abstracts

Author

Dunet, V., primary, Dabiri, A., additional, Allenbach, G., additional, Goyeneche Achigar, A., additional, Waeber, B., additional, Feihl, F., additional, Heinzer, R., additional, Prior, J. O., additional, Van Velzen, J. E., additional, Schuijf, J. D., additional, De Graaf, F. R., additional, De Graaf, M. A., additional, Schalij, M. J., additional, Kroft, L. J., additional, De Roos, A., additional, Jukema, J. W., additional, Van Der Wall, E. E., additional, Bax, J. J., additional, Lankinen, E., additional, Saraste, A., additional, Noponen, T., additional, Klen, R., additional, Teras, M., additional, Kokki, T., additional, Kajander, S., additional, Pietila, M., additional, Ukkonen, H., additional, Knuuti, J., additional, Pazhenkottil, A. P., additional, Nkoulou, R. N., additional, Ghadri, J. R., additional, Herzog, B. A., additional, Buechel, R. R., additional, Kuest, S. M., additional, Wolfrum, M., additional, Gaemperli, O., additional, Husmann, L., additional, Kaufmann, P. A., additional, Andreini, D., additional, Pontone, G., additional, Mushtaq, S., additional, Antonioli, L., additional, Bertella, E., additional, Formenti, A., additional, Cortinovis, S., additional, Ballerini, G., additional, Fiorentini, C., additional, Pepi, M., additional, Koh, A. S., additional, Flores, J. S., additional, Keng, F. Y. J., additional, Tan, R. S., additional, Chua, T. S. J., additional, Annoni, A. D., additional, Tamborini, G., additional, Fusari, M., additional, Bartorelli, A. L., additional, Ewe, S. H., additional, Ng, A. C. T., additional, Delgado, V., additional, Schuijf, J., additional, Van Der Kley, F., additional, Colli, A., additional, De Weger, A., additional, Marsan, N. A., additional, Yiu, K. H., additional, Ng, A. C., additional, Timmer, S. A. J., additional, Knaapen, P., additional, Germans, T., additional, Dijkmans, P. A., additional, Lubberink, M., additional, Ten Berg, J. M., additional, Ten Cate, F. J., additional, Russel, I. K., additional, Lammertsma, A. A., additional, Van Rossum, A. C., additional, Wong, Y. Y., additional, Ruiter, G., additional, Raijmakers, P., additional, Van Der Laarse, W. J., additional, Westerhof, N., additional, Vonk-Noordegraaf, A., additional, Youssef, G., additional, Leung, E., additional, Wisenberg, G., additional, Marriot, C., additional, Williams, K., additional, Etele, J., additional, Dekemp, R. A., additional, Dasilva, J., additional, Birnie, D., additional, Beanlands, R. S. B., additional, Thompson, R. C., additional, Allam, A. H., additional, Wann, L. S., additional, Nureldin, A. H., additional, Adelmaksoub, G., additional, Badr, I., additional, Sutherland, M. L., additional, Sutherland, J. D., additional, Miyamoto, M. I., additional, Thomas, G. S., additional, Harms, H. J., additional, De Haan, S., additional, Huisman, M. C., additional, Schuit, R. C., additional, Windhorst, A. D., additional, Allaart, C., additional, Einstein, A. J., additional, Khawaja, T., additional, Greer, C., additional, Chokshi, A., additional, Jones, M., additional, Schaefle, K., additional, Bhatia, K., additional, Shimbo, D., additional, Schulze, P. C., additional, Srivastava, A., additional, Chettiar, R., additional, Moody, J., additional, Weyman, C., additional, Natale, D., additional, Bruni, W., additional, Liu, Y., additional, Ficaro, E., additional, Sinusas, A. J., additional, Peix, A., additional, Batista, E., additional, Cabrera, L. O., additional, Padron, K., additional, Rodriguez, L., additional, Sainz, B., additional, Mendoza, V., additional, Carrillo, R., additional, Fernandez, Y., additional, Mena, E., additional, Naum, A., additional, Bach-Gansmo, T., additional, Kleven-Madsen, N., additional, Biermann, M., additional, Johnsen, B., additional, Aase Husby, J., additional, Rotevatn, S., additional, Nordrehaug, J. E., additional, Schaap, J., additional, Kauling, R. M., additional, Post, M. C., additional, Rensing, B. J. W. M., additional, Verzijlbergen, J. F., additional, Sanchez, J., additional, Giamouzis, G., additional, Tziolas, N., additional, Georgoulias, P., additional, Karayannis, G., additional, Chamaidi, A., additional, Zavos, N., additional, Koutrakis, K., additional, Sitafidis, G., additional, Skoularigis, J., additional, Triposkiadis, F., additional, Radovanovic, S., additional, Djokovic, A., additional, Simic, D. V., additional, Krotin, M., additional, Savic-Radojevic, A., additional, Pljesa-Ercegovac, M., additional, Zdravkovic, M., additional, Saponjski, J., additional, Jelic, S., additional, Simic, T., additional, Eckardt, R., additional, Kjeldsen, B. J., additional, Andersen, L. I., additional, Haghfelt, T., additional, Grupe, P., additional, Johansen, A., additional, Hesse, B., additional, Pena, H., additional, Cantinho, G., additional, Wilk, M., additional, Srour, Y., additional, Godinho, F., additional, Zafrir, N., additional, Gutstein, A., additional, Mats, I., additional, Battler, A., additional, Solodky, A., additional, Sari, E., additional, Singh, N., additional, Vara, A., additional, Peters, A. M., additional, De Belder, A., additional, Nair, S., additional, Ryan, N., additional, James, R., additional, Dizdarevic, S., additional, Depuey, G., additional, Friedman, M., additional, Wray, R., additional, Old, R., additional, Babla, H., additional, Chuanyong, B., additional, Maddahi, J., additional, Tragardh Johansson, E., additional, Sjostrand, K., additional, Edenbrandt, L., additional, Aguade-Bruix, S., additional, Cuberas-Borros, G., additional, Pizzi, M. N., additional, Sabate-Fernandez, M., additional, De Leon, G., additional, Garcia-Dorado, D., additional, Castell-Conesa, J., additional, Candell-Riera, J., additional, Casset-Senon, D., additional, Edjlali-Goujon, M., additional, Alison, D., additional, Delhommais, A., additional, Cosnay, P., additional, Low, C. S., additional, Notghi, A., additional, O'brien, J., additional, Tweddel, A. C., additional, Bingham, N., additional, O Neil, P., additional, Harbinson, M., additional, Lindner, O., additional, Burchert, W., additional, Schaefers, M., additional, Marcassa, C., additional, Campini, R., additional, Calza, P., additional, Zoccarato, O., additional, Kisko, A., additional, Kmec, J., additional, Babcak, M., additional, Vereb, M., additional, Vytykacova, M., additional, Cencarik, J., additional, Gazdic, P., additional, Stasko, J., additional, Abreu, A., additional, Pereira, E., additional, Oliveira, L., additional, Colarinha, P., additional, Veloso, V., additional, Enriksson, I., additional, Proenca, G., additional, Delgado, P., additional, Rosario, L., additional, Sequeira, J., additional, Kosa, I., additional, Vassanyi, I., additional, Egyed, C. S., additional, Kozmann, G. Y., additional, Morita, S., additional, Nanasato, M., additional, Nanbu, I., additional, Yoshida, Y., additional, Hirayama, H., additional, Allam, A., additional, Sharef, A., additional, Shawky, I., additional, Farid, M., additional, Mouden, M., additional, Ottervanger, J. P., additional, Timmer, J. R., additional, De Boer, M. J., additional, Reiffers, S., additional, Jager, P. L., additional, Knollema, S., additional, Nasr, G. M., additional, Mohy Eldin, M., additional, Ragheb, M., additional, Casans-Tormo, I., additional, Diaz-Exposito, R., additional, Hurtado-Mauricio, F. J., additional, Ruano, R., additional, Diego, M., additional, Gomez-Caminero, F., additional, Albarran, C., additional, Martin De Arriba, A., additional, Rosero, A., additional, Lopez, R., additional, Martin Luengo, C., additional, Garcia-Talavera, J. R., additional, Laitinen, I. E. K., additional, Rudelius, M., additional, Weidl, E., additional, Henriksen, G., additional, Wester, H. J., additional, Schwaiger, M., additional, Pan, X. B., additional, Schindler, T., additional, Quercioli, A., additional, Zaidi, H., additional, Ratib, O., additional, Declerck, J. M., additional, Alexanderson Rosas, E., additional, Jacome, R., additional, Jimenez-Santos, M., additional, Romero, E., additional, Pena-Cabral, M. A., additional, Meave, A., additional, Gonzalez, J., additional, Rouzet, F., additional, Bachelet, L., additional, Alsac, J. M., additional, Suzuki, M., additional, Louedec, L., additional, Petiet, A., additional, Chaubet, F., additional, Letourneur, D., additional, Michel, J. B., additional, Le Guludec, D., additional, Aktas, A., additional, Cinar, A., additional, Yaman, G., additional, Bahceci, T., additional, Kavak, K., additional, Gencoglu, A., additional, Jimenez-Heffernan, A., additional, Sanchez De Mora, E., additional, Lopez-Martin, J., additional, Lopez-Aguilar, R., additional, Ramos, C., additional, Salgado, C., additional, Ortega, A., additional, Sanchez-Gonzalez, C., additional, Roa, J., additional, Tobaruela, A., additional, Nesterov, S. V., additional, Turta, O., additional, Maki, M., additional, Han, C., additional, Daou, D., additional, Tawileh, M., additional, Chamouine, S. O., additional, Coaguila, C., additional, Mariscal-Labrador, E., additional, Kisiel-Gonzalez, N., additional, De Araujo Goncalves, P., additional, Sousa, P. J., additional, Marques, H., additional, O'neill, J., additional, Pisco, J., additional, Cale, R., additional, Brito, J., additional, Gaspar, A., additional, Machado, F. P., additional, Roquette, J., additional, Martinez, M., additional, Melendez, G., additional, Kimura, E., additional, Ochoa, J. M., additional, Alessio, A. M., additional, Patel, A., additional, Lautamaki, R., additional, Bengel, F. M., additional, Bassingthwaighte, J. B., additional, Caldwell, J. H., additional, Rahbar, K., additional, Seifarth, H., additional, Schafers, M., additional, Stegger, L., additional, Spieker, T., additional, Hoffmeier, A., additional, Maintz, D., additional, Scheld, H., additional, Schober, O., additional, Weckesser, M., additional, Aoki, H., additional, Matsunari, I., additional, Kajinami, K., additional, Martin Fernandez, M., additional, Barreiro Perez, M., additional, Fernandez Cimadevilla, O. V., additional, Leon Duran, D., additional, Velasco Alonso, E., additional, Florez Munoz, J. P., additional, Luyando, L. H., additional, Templin, C., additional, Veltman, C. E., additional, Reiber, J. H. C., additional, Venuraju, S., additional, Yerramasu, A., additional, Atwal, S., additional, Lahiri, A., additional, Kunimasa, T., additional, Shiba, M., additional, Ishii, K., additional, Aikawa, J., additional, Kroner, E. S. J., additional, Ho, K. T., additional, Yong, Q. W., additional, Chua, K. C., additional, Panknin, C., additional, Roos, C. J., additional, Van Werkhoven, J. M., additional, Witkowska-Grzeslo, A. J., additional, Boogers, M. J., additional, Anand, D. V., additional, Dey, D., additional, Berman, D., additional, Mut, F., additional, Giubbini, R., additional, Lusa, L., additional, Massardo, T., additional, Iskandrian, A., additional, Dondi, M., additional, Sato, A., additional, Kakefuda, Y., additional, Ojima, E., additional, Adachi, T., additional, Atsumi, A., additional, Ishizu, T., additional, Seo, Y., additional, Hiroe, M., additional, Aonuma, K., additional, Kruk, M., additional, Pracon, R., additional, Kepka, C., additional, Pregowski, J., additional, Kowalewska, A., additional, Pilka, M., additional, Opolski, M., additional, Michalowska, I., additional, Dzielinska, Z., additional, Demkow, M., additional, Stoll, V., additional, Sabharwal, N., additional, Chakera, A., additional, Ormerod, O., additional, Fernandes, H., additional, Bernardes, M., additional, Martins, E., additional, Oliveira, P., additional, Vieira, T., additional, Terroso, G., additional, Oliveira, A., additional, Faria, T., additional, Ventura, F., additional, Pereira, J., additional, Fukuzawa, S., additional, Inagaki, M., additional, Sugioka, J., additional, Ikeda, A., additional, Okino, S., additional, Maekawa, J., additional, Uchiyama, T., additional, Kamioka, N., additional, Ichikawa, S., additional, Afshar, M., additional, Alvi, R., additional, Aguilar, N., additional, Ippili, R., additional, Shaqra, H., additional, Bella, J., additional, Bhalodkar, N., additional, Dos Santos, A., additional, Daicz, M., additional, Cendoya, L. O., additional, Marrero, H. G., additional, Casuscelli, J., additional, Embon, M., additional, Vera Janavel, G., additional, Duronto, E., additional, Gurfinkel, E. P., additional, Cortes, C. M., additional, Takeishi, Y., additional, Nakajima, K., additional, Yamasaki, Y., additional, Nishimura, T., additional, Hayes Brown, K., additional, Collado, F., additional, Alhaji, M., additional, Green, J., additional, Alexander, S., additional, Vashistha, R., additional, Jain, S., additional, Aldaas, F., additional, Shanes, J., additional, Doukky, R., additional, Ashikaga, K., additional, Akashi, Y. J., additional, Uemarsu, M., additional, Kamijima, R., additional, Yoneyama, K., additional, Omiya, K., additional, Miyake, Y., additional, Brodov, Y., additional, Raval, U., additional, Berezin, A., additional, Seden, V., additional, Koretskaya, E., additional, Panasenko, T. A., additional, Matsuo, S., additional, Kinuya, S., additional, Chen, J., additional, Van Bommel, R. J., additional, Van Der Hiel, B., additional, Dibbets-Schneider, P., additional, Garcia, E. V., additional, Rutten-Vermeltfoort, I., additional, Gevers, M. M. J., additional, Verhoeven, B., additional, Dijk Van, A. B., additional, Raaijmakers, E., additional, Raijmakers, P. G. H. M., additional, Engvall, J. E., additional, Gjerde, M., additional, De Geer, J., additional, Olsson, E., additional, Quick, P., additional, Persson, A., additional, Mazzanti, M., additional, Marini, M., additional, Pimpini, L., additional, Perna, G. P., additional, Marciano, C., additional, Gargiulo, P., additional, Galderisi, M., additional, D'amore, C., additional, Savarese, G., additional, Casaretti, L., additional, Paolillo, S., additional, Cuocolo, A., additional, Perrone Filardi, P., additional, Al-Amoodi, M., additional, Thompson, E. C., additional, Kennedy, K., additional, Bybee, K. A., additional, Mcghie, A. I., additional, O'keefe, J. H., additional, Bateman, T. M., additional, Van Der Palen, R. L. F., additional, Mavinkurve-Groothuis, A. M., additional, Bulten, B., additional, Bellersen, L., additional, Van Laarhoven, H. W. M., additional, Kapusta, L., additional, De Geus-Oei, L. F., additional, Pollice, P. P., additional, Bonifazi, M. B., additional, Pollice, F. P., additional, Clements, I. P., additional, Hodge, D. O., additional, Scott, C. G., additional, De Ville De Goyet, M., additional, Brichard, B., additional, Pirotte, T., additional, Moniotte, S., additional, Tio, R. A., additional, Elvan, A., additional, Dierckx, R. A. I. O., additional, Slart, R. H. J. A., additional, Furuhashi, T., additional, Moroi, M., additional, Hase, H., additional, Joki, N., additional, Masai, H., additional, Nakazato, R., additional, Fukuda, H., additional, Sugi, K., additional, Kryczka, K., additional, Kaczmarska, E., additional, Petryka, J., additional, Mazurkiewicz, L., additional, Ruzyllo, W., additional, Smanio, P., additional, Vieira Segundo, E., additional, Siqueira, M., additional, Kelendjian, J., additional, Ribeiro, J., additional, Alaca, J., additional, Oliveira, M., additional, Alves, F., additional, Peovska, I., additional, Maksimovic, J., additional, Vavlukis, M., additional, Kostova, N., additional, Pop Gorceva, D., additional, Majstorov, V., additional, Zdraveska, M., additional, Hussain, S., additional, Djearaman, M., additional, Hoey, E., additional, Morus, L., additional, Erinfolami, O., additional, Macnamara, A., additional, Opolski, M. P., additional, Witkowski, A., additional, Berti, V., additional, Ricci, F., additional, Gallicchio, R., additional, Acampa, W., additional, Cerisano, G., additional, Vigorito, C., additional, Sciagra', R., additional, Pupi, A., additional, Sliem, H., additional, Collado, F. M., additional, Schmidt, S., additional, Maheshwari, A., additional, Kiriakos, R., additional, Mwansa, V., additional, Ljubojevic, S., additional, Sedej, S., additional, Holzer, M., additional, Marsche, G., additional, Marijanski, V., additional, Kockskaemper, J., additional, Pieske, B., additional, Ricalde, A., additional, Alexanderson, G., additional, Mohani, A., additional, Khanna, P., additional, Sinusas, A., additional, Lee, F., additional, Pinas, V. A., additional, Van Eck-Smit, B. L. F., additional, Verberne, H. J., additional, De Bruin, C. M., additional, Guilhermina, G., additional, Jimenez-Angeles, L., additional, Ruiz De Jesus, O., additional, Yanez-Suarez, O., additional, Vallejo, E., additional, Reyes, E., additional, Chan, M., additional, Hossen, M. L., additional, Underwood, S. R., additional, Karu, A., additional, Bokhari, S., additional, Pineda, V., additional, Gracia-Sanchez, L. M., additional, Garcia-Burillo, A., additional, Zavadovskiy, K., additional, Lishmanov, Y. U., additional, Saushkin, W., additional, Kovalev, I., additional, Chernishov, A., additional, Annoni, A., additional, Tarkia, M., additional, Saanijoki, T., additional, Oikonen, V., additional, Savunen, T., additional, Green, M. A., additional, Strandberg, M., additional, Roivainen, A., additional, Gaeta, M. C., additional, Artigas, C., additional, Deportos, J., additional, Geraldo, L., additional, Flotats, A., additional, La Delfa, V., additional, Carrio, I., additional, Laarse, W. J., additional, Izquierdo Gomez, M. M., additional, Lacalzada Almeida, J., additional, Barragan Acea, A., additional, De La Rosa Hernandez, A., additional, Juarez Prera, R., additional, Blanco Palacios, G., additional, Bonilla Arjona, J. A., additional, Jimenez Rivera, J. J., additional, Iribarren Sarrias, J. L., additional, Laynez Cerdena, I., additional, Dedic, A., additional, Rossi, A., additional, Ten Kate, G. J. R., additional, Dharampal, A., additional, Moelker, A., additional, Galema, T. W., additional, Mollet, N., additional, De Feyter, P. J., additional, Nieman, K., additional, Trabattoni, D., additional, Broersen, A., additional, Frenay, M., additional, Boogers, M. M., additional, Kitslaar, P. H., additional, Dijkstra, J., additional, Annoni, D. A., additional, Muratori, M., additional, Johki, N., additional, Tokue, M., additional, Dharampal, A. S., additional, Weustink, A. C., additional, Neefjes, L. A. E., additional, Papadopoulou, S. L., additional, Chen, C., additional, Mollet, N. R. A., additional, Boersma, E. H., additional, Krestin, G. P., additional, Purvis, J. A., additional, Sharma, D., additional, Hughes, S. M., additional, Berman, D. S., additional, Taillefer, R., additional, Udelson, J., additional, Devine, M., additional, Lazewatsky, J., additional, Bhat, G., additional, Washburn, D., additional, Patel, D., additional, Mazurek, T., additional, Tandon, S., additional, Bansal, S., additional, Inzucchi, S., additional, Staib, L., additional, Davey, J., additional, Chyun, D., additional, Young, L., additional, Wackers, F., additional, Harbinson, M. T., additional, Wells, G., additional, Dougan, J., additional, Borges-Neto, S., additional, Phillips, H., additional, Farzaneh-Far, A., additional, Starr, Z., additional, Shaw, L. K., additional, Fiuzat, M., additional, O'connor, C., additional, Henzlova, M., additional, Duvall, W. L., additional, Levine, A., additional, Baber, U., additional, Croft, L., additional, Sahni, S., additional, Sethi, S., additional, Hermann, L., additional, Nureldin, A., additional, Gomaa, A., additional, Soliman, M. A. T., additional, Hany, H. A. R., additional, De Graaf, F., additional, Pazhenkottil, A., additional, Siebelink, H. M. J., additional, Reiber, J. H., additional, Ayub, M., additional, Naveed, T., additional, Azhar, M., additional, Van Tosh, A., additional, Faber, T. L., additional, Votaw, J. R., additional, Reichek, N., additional, Pulipati, B., additional, Palestro, C., additional, Nichols, K. J., additional, Okuda, K., additional, Kirihara, Y., additional, Ishikawa, T., additional, Taki, J., additional, Yoshita, M., additional, Yamada, M., additional, Salacata, A., additional, Keavey, S., additional, Chavarri, V., additional, Mills, J., additional, Nagaraj, H., additional, Bhambhani, P., additional, Kliner, D. E., additional, Soman, P., additional, Heo, J., additional, Iskandrian, A. E., additional, Jain, M., additional, Lin, B., additional, Walker, A., additional, Nkonde, C., additional, Bond, S., additional, Baskin, A., additional, Declerck, J., additional, Soto, M. E., additional, Mendoza, G., additional, Aguilar, M., additional, Williams, S. P., additional, Colice, G., additional, Mcardle, J. R., additional, Lankford, A., additional, Kajdasz, D. K., additional, Reed, C. R., additional, Angelini, L., additional, Angelozzi, F., additional, Ascoli, G., additional, Jacobson, A., additional, Lessig, H. J., additional, Gerson, M. C., additional, Cerqueira, M. D., additional, Narula, J., additional, Uematsu, M., additional, Kida, K., additional, Suzuki, K., additional, Bravo, P. E., additional, Fukushima, K., additional, Chaudhry, M., additional, Merrill, J., additional, Alonso Tello, A., additional, Rodriguez Palomares, J. F., additional, Marti Aguasca, G., additional, Aguade Bruix, S., additional, Aliaga, V., additional, Mahia, P., additional, Gonzalez-Alujas, T., additional, Candell, J., additional, Evangelista, A., additional, Mlynarski, R., additional, Mlynarska, A., additional, Sosnowski, M., additional, Zerahn, B., additional, Hasbak, P., additional, Mortensen, C. E., additional, Mathiesen, H. F., additional, Andersson, M., additional, Nielsen, D., additional, Ferreira Santos, L., additional, Ferreira, M. J., additional, Ramos, D., additional, Moreira, D., additional, Cunha, M. J., additional, Albuquerque, A., additional, Moreira, A., additional, Oliveira Santos, J., additional, Costa, G., additional, Providencia, L. A., additional, Arita, Y., additional, Kihara, S., additional, Mitsusada, N., additional, Miyawaki, M., additional, Ueda, H., additional, Hiraoka, H., additional, Matsuzawa, Y., additional, Askew, J., additional, O'connor, M., additional, Jordan, L., additional, Ruter, R., additional, Gibbons, R., additional, Miller, T., additional, Emmett, L., additional, Ng, A., additional, Sorensen, N., additional, Mansberg, R., additional, Kritharides, L., additional, Gonzalez, T., additional, Majmundar, H., additional, Coats, N. P., additional, Vernotico, S., additional, Doan, J. H., additional, Hernandez, T. M., additional, Evini, M., additional, Hepner, A. D., additional, Ip, T. K., additional, Chalela, W. A., additional, Falcao, A. M., additional, Azouri, L. O., additional, Ramires, J. A. F., additional, Meneghetti, J. C., additional, Manganelli, F., additional, Spadafora, M., additional, Varrella, P., additional, Peluso, G., additional, Sauro, R., additional, Di Lorenzo, E., additional, Rotondi, F., additional, Daniele, S., additional, Miletto, P., additional, Rijnders, A. J. M., additional, Hendrickx, B. W., additional, Van Der Bruggen, W., additional, America, Y. G. C. J., additional, Thorley, P. J., additional, Chowdhury, F. U., additional, Dickinson, C. J., additional, Sazonova, S. I., additional, Proskokova, I. Y. U., additional, Gusakova, A. M., additional, Minin, S. M., additional, Lishmanov, Y. U. B., additional, Saushkin, V. V., additional, Rodriguez, G., additional, Roffe, F., additional, Ilarraza, H., additional, Bialostozky, D., additional, Kitsiou, A. N., additional, Arsenos, P., additional, Tsiantis, I., additional, Charizopoulos, S., additional, Karas, S., additional, Vidal Perez, R. C., additional, Garrido, M., additional, Pubul, V., additional, Argibay, S., additional, Pena, C., additional, Pombo, M., additional, Ciobotaru, A. B., additional, Sanchez-Salmon, A., additional, Ruibal Morell, A., additional, Gonzalez-Juanatey, J. R., additional, Rodriguez-Gomez, E., additional, Martinez, B., additional, Pontillo, D., additional, Benvissuto, F., additional, Fiore Melacrinis, F., additional, Maccafeo, S., additional, Scabbia, E. V., additional, Schiavo, R., additional, Golzar, Y., additional, Gidea, C., additional, Golzar, J., additional, Pop-Gorceva, D., additional, Zdravkovska, M., additional, Stojanovski, S., additional, Georgievska-Ismail, L. J., additional, Katsikis, T., additional, Theodorakos, A., additional, Kouzoumi, A., additional, Koutelou, M., additional, Yoshimura, Y., additional, Toyama, T., additional, Hoshizaki, H., additional, Ohshima, S., additional, Inoue, M., additional, Suzuki, T., additional, Uitterdijk, A., additional, Dijkshoorn, M., additional, Van Straten, M., additional, Van Der Giessen, W. J., additional, Duncker, D. J., additional, Merkus, D., additional, Platsch, G., additional, Sunderland, J., additional, Tonge, C., additional, Arumugam, P., additional, Dey, T., additional, Wieczorek, H., additional, Bippus, R., additional, Romijn, R. L., additional, Backus, B. E., additional, Aach, T., additional, Lomsky, M., additional, Johansson, L., additional, Marving, J., additional, Svensson, S., additional, Pou, J. L., additional, Esteves, F. P., additional, Raggi, P., additional, Folks, R., additional, Keidar, Z., additional, Askew, J. W., additional, Verdes, L., additional, Campos, L., additional, Gulyaev, V., additional, Pankova, A., additional, Santos, J., additional, Carmona, S., additional, Henriksson, I., additional, Prata, A., additional, Carrageta, M., additional, Santos, A. I., additional, Yoshinaga, K., additional, Naya, M., additional, Katoh, C., additional, Manabe, O., additional, Yamada, S., additional, Iwano, H., additional, Chiba, S., additional, Tsutsui, H., additional, Tamaki, N., additional, Vassiliadis, I., additional, Despotopoulos, E., additional, Kaitozis, O., additional, Hatzistamatiou, E., additional, Thompson, R., additional, Hatch, J., additional, Zink, M., additional, Gu, B. S., additional, Bae, G. D., additional, Dae, C. M., additional, Min, G. H., additional, Chun, E. J., additional, Choi, S. I., additional, Al-Mallah, M., additional, Kassem, K., additional, Khawaja, O., additional, Goodman, D., additional, Lipkin, D., additional, Christiaens, L., additional, Bonnet, B., additional, Mergy, J., additional, Coisne, D., additional, Allal, J., additional, Dias Ferreira, N., additional, Leite, D., additional, Rocha, J., additional, Carvalho, M., additional, Caeiro, D., additional, Bettencourt, N., additional, Braga, P., additional, Gama Ribeiro, V., additional, Kristoffersen, U. S., additional, Lebech, A. M., additional, Gutte, H., additional, Ripa, R. S., additional, Wiinberg, N., additional, Petersen, C. L., additional, Jensen, G., additional, Kjaer, A., additional, Bai, C., additional, Conwell, R., additional, Folks, R. D., additional, Verdes-Moreiras, L., additional, Manatunga, D., additional, Jacobson, A. F., additional, Belzer, D., additional, Hasid, Y., additional, Rehling, M., additional, Poulsen, R. H., additional, Falborg, L., additional, Rasmussen, J. T., additional, Waehrens, L. N., additional, Heegaard, C. W., additional, Silvola, J. M. U., additional, Forsback, S., additional, Laine, J. O., additional, Heinonen, S., additional, Ylaherttuala, S., additional, Broisat, A., additional, Ruiz, M., additional, Goodman, N. C., additional, Dimastromatteo, J., additional, Glover, D. K., additional, Hyafil, F., additional, Blackwell, F., additional, Pavon-Djavid, G., additional, Sarda-Mantel, L., additional, Feldman, L. J., additional, Meddahi-Pelle, A., additional, Tsatkin, V., additional, Liu, Y.- H., additional, De Kemp, R., additional, Slomka, P. J., additional, Klein, R., additional, Germano, G., additional, Beanlands, R. S., additional, Rohani, A., additional, Akbari, V., additional, Groothuis, J. G. J., additional, Fransen, M., additional, Beek, A. M., additional, Brinckman, S. L., additional, Meijerink, M. R., additional, Hofman, M. B. M., additional, Van Kuijk, C., additional, Kogure, S., additional, Yamashita, E., additional, Murakami, J., additional, Kawaguchi, R., additional, Adachi, H., additional, Oshima, S., additional, Minin, S., additional, Popov, S., additional, Saushkina, Y. U., additional, Savenkova, G., additional, Lebedev, D., additional, Alexandridis, E., additional, Rovithis, D., additional, Parisis, C., additional, Sazonova, I., additional, Saushkin, V., additional, Chernov, V., additional, Zaabar, L., additional, Bahri, H., additional, Hadj Ali, S., additional, Sellem, A., additional, Slim, I., additional, El Kadri, N., additional, Slimen, H., additional, Hammami, H., additional, Lucic, S., additional, Peter, A., additional, Tadic, S., additional, Nikoletic, K., additional, Jung, R., additional, Lucic, M., additional, Tagil, K., additional, Jakobsson, D., additional, Svensson, S.- E., additional, Wollmer, P., additional, Leccisotti, L., additional, Indovina, L., additional, Paraggio, L., additional, Calcagni, M. L., additional, Giordano, A., additional, Kapitan, M., additional, Paolino, A., additional, Nunez, M., additional, Sweeny, J., additional, Kulkarni, N., additional, Guma, K., additional, Akashi, Y., additional, Takano, M., additional, Takai, M., additional, Koh, S., additional, Miyake, F., additional, Torun, N., additional, Durmus Altun, G., additional, Altun, A., additional, Kaya, E., additional, Saglam, H., additional, Matsuoka, D. T., additional, Sanchez, A., additional, Bartolozzi, C., additional, Padua, D., additional, Ponta, G., additional, Ponte, A., additional, Carneiro, A., additional, Thom, A., additional, Ashrafi, R., additional, Garg, P., additional, Davis, G., additional, Falcao, A., additional, Costa, M., additional, Bussolini, F., additional, Meneghetti, J. A. C., additional, Tobisaka, M., additional, Correia, E., additional, Jansen, J. W., additional, Van Der Vleuten, P. A., additional, Willems, T. P., additional, Zijlstra, F., additional, Sato, M., additional, Taniguchi, K., additional, Kurabayashi, M., additional, Pop Gjorcheva, D., additional, Zdraveska-Kochovska, M., additional, Moriwaki, K., additional, Kawamura, A., additional, Watanabe, K., additional, Omura, T., additional, Sakabe, S., additional, Seko, T., additional, Kasai, A., additional, Ito, M., additional, Obana, M., additional, Akasaka, T., additional, Hruska, C., additional, Truong, D., additional, Pletta, C., additional, Collins, D., additional, Tortorelli, C., additional, Rhodes, D., additional, El-Prince, M., additional, Martinez-Moeller, A., additional, Marinelli, M., additional, Weismueller, S., additional, Hillerer, C., additional, Jensen, B., additional, Nekolla, S. G., additional, Wakabayashi, H., additional, Tsukamoto, K., additional, Baker, S. M. E. A., additional, Sirajul Haque, K. M. H. S., additional, Siddique, A., additional, Krishna Banarjee, S., additional, Ahsan, A., additional, Rahman, F., additional, Mukhlesur Rahman, M., additional, Parveen, T., additional, Lutfinnessa, M., additional, Nasreen, F., additional, Sano, H., additional, Naito, S., additional, De Rimini, M. L., additional, Borrelli, G., additional, Baldascino, F., additional, Calabro, P., additional, Maiello, C., additional, Russo, A., additional, Amarelli, C., additional, Muto, P., additional, Danad, I., additional, Raijmakers, P. G., additional, Appelman, Y. E., additional, Hoekstra, O. S., additional, Marcus, J. T., additional, Boonstra, A., additional, Ryzhkova, D. V., additional, Kuzmina, T. V., additional, Borodina, O. S., additional, Trukshina, M. A., additional, Kostina, I. S., additional, Hommel, H., additional, Feuchtner, G., additional, Pachinger, O., additional, Friedrich, G., additional, Stel, A. M., additional, Deckers, J. W., additional, Gama, V., additional, Ciarka, A., additional, Neefjes, L. A., additional, Mollet, N. R., additional, Sijbrands, E. J., additional, Wilczek, J., additional, Llibre Pallares, C., additional, Abdul-Jawad Altisent, O., additional, Cuellar Calabria, H., additional, Mahia Casado, P., additional, Gonzalez-Alujas, M. T., additional, Evangelista Masip, A., additional, Garcia-Dorado Garcia, D., additional, Tekabe, Y., additional, Shen, X., additional, Li, Q., additional, Luma, J., additional, Weisenberger, D., additional, Schmidt, A. M., additional, Haubner, R., additional, Johnson, L., additional, Sleiman, L., additional, Thorn, S., additional, Hasu, M., additional, Thabet, M., additional, Dasilva, J. N., additional, Whitman, S. C., additional, Genovesi, D., additional, Giorgetti, A., additional, Gimelli, A., additional, Cannizzaro, G., additional, Bertagna, F., additional, Fagioli, G., additional, Rossi, M., additional, Bonini, R., additional, Marzullo, P., additional, Paterson, C. A., additional, Smith, S. A., additional, Small, A. D., additional, Goodfield, N. E. R., additional, Martin, W., additional, Nekolla, S., additional, Sherif, H., additional, Reder, S., additional, Yu, M., additional, Kusch, A., additional, Li, D., additional, Zou, J., additional, Lloyd, M. S., additional, Cao, K., additional, Motherwell, D. W., additional, Rice, A., additional, Mccurrach, G. M., additional, Cobbe, S. M., additional, Petrie, M. C., additional, Al Younis, I., additional, Van Der Wall, E., additional, Mirza, T., additional, Raza, M., additional, Hashemizadeh, H., additional, Santos, L., additional, Krishna, B. A., additional, Perna, F., additional, Lago, M., additional, Leo, M., additional, Pelargonio, G., additional, Bencardino, G., additional, Narducci, M. L., additional, Casella, M., additional, Bellocci, F., additional, Kirac, S., additional, Yaylali, O., additional, Serteser, M., additional, Yaylali, T., additional, Okizaki, A., additional, Urano, Y., additional, Nakayama, M., additional, Ishitoya, S., additional, Sato, J., additional, Ishikawa, Y., additional, Sakaguchi, M., additional, Nakagami, N., additional, Aburano, T., additional, Solav, S. V., additional, Bhandari, R., additional, Burrell, S., additional, Dorbala, S., additional, Bruno, I., additional, Caldarella, C., additional, Collarino, A., additional, Mattoli, M. V., additional, Stefanelli, A., additional, Cannarile, A., additional, Maggi, F., additional, Soukhov, V., additional, Bondarev, S., additional, Yalfimov, A., additional, Khan, M., additional, Priyadharshan, P. P., additional, Chandok, G., additional, Aziz, T., additional, Avison, M., additional, Smith, R. A., additional, Bulugahapitya, D. S., additional, Vakhtangadze, T., additional, Todua, F., additional, Baramia, M., additional, Antelava, G., additional, Roche, N.- C., additional, Paule, P., additional, Kerebel, S., additional, Gil, J.- M., additional, Fourcade, L., additional, Tzonevska, A., additional, Tzvetkov, K., additional, Atanasova, M., additional, Parvanova, V., additional, Chakarova, A., additional, Piperkova, E., additional, Kocabas, B., additional, Muderrisoglu, H., additional, Allaart, C. P., additional, Entok, E., additional, Simsek, S., additional, Akcay, B., additional, Ak, I., additional, Vardareli, E., additional, Stachura, M., additional, Kwasiborski, P. J., additional, Horszczaruk, G. J., additional, Komar, E., additional, Cwetsch, A., additional, Zraik, B., additional, Morales Demori, R., additional, Almeida, A. D. J., additional, Siqueira, M. E., additional, Vieira, E., additional, Balogh, I., additional, Kerecsen, G., additional, Marosi, E., additional, Szelid, Z. S., additional, Sattar, A., additional, Swadia, T., additional, Chattahi, J., additional, Qureshi, W., additional, Khalid, F., additional, Gonzalez, A., additional, Hechavarria, S., additional, Takamura, K., additional, Fujimoto, S., additional, Nakanishi, R., additional, Yamashina, S., additional, Namiki, A., additional, Yamazaki, J., additional, Koshino, K., additional, Hashikawa, Y., additional, Teramoto, N., additional, Hikake, M., additional, Ishikane, S., additional, Ikeda, T., additional, Iida, H., additional, Takahashi, Y., additional, Oriuchi, N., additional, Higashino, H., additional, Endo, K., additional, Mochizuki, T., additional, Murase, K., additional, Baali, A., additional, Moreno, R., additional, Chau, M., additional, Rousseau, H., additional, Nicoud, F., additional, Dolliner, P., additional, Brammen, L., additional, Steurer, G., additional, Traub-Weidinger, T., additional, Ubl, P., additional, Schaffarich, P., additional, Dobrozemsky, G., additional, Staudenherz, A., additional, Ozgen Kiratli, M., additional, Temelli, B., additional, Kanat, N. B., additional, Aksoy, T., additional, Slavich, G. A., additional, Piccoli, G., additional, Puppato, M., additional, Grillone, S., additional, Gasparini, D., additional, Dunet, V., additional, Perruchoud, S., additional, Poitry-Yamate, C., additional, Lepore, M., additional, Gruetter, R., additional, Pedrazzini, T., additional, Anselm, D., additional, Anselm, A., additional, Atkins, H., additional, Renaud, J., additional, Dekemp, R., additional, Burwash, I., additional, Guo, A., additional, Beanlands, R., additional, Glover, C., additional, Vilardi, I., additional, Zangheri, B., additional, Calabrese, L., additional, Romano, P., additional, Bruno, A., additional, Fernandez Cimadevilla, O. C., additional, Uusitalo, V. A., additional, Luotolahti, M., additional, Wendelin-Saarenhovi, M., additional, Sundell, J., additional, Raitakari, O., additional, Huidu, S., additional, Gadiraju, R., additional, Ghesani, M., additional, Uddin, Q., additional, Wosnitzer, B., additional, Takahashi, N., additional, Alhaj, E., additional, Legasto, A., additional, Abiri, B., additional, Elsaban, K., additional, El Khouly, T., additional, El Kammash, T., additional, Al Ghamdi, A., additional, Kyung Deok, B., additional, Bon Seung, K., additional, Sang Geun, Y., additional, Chang Min, D., additional, and Gwan Hong, M., additional

Published
2011
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56. Fractional composition of grain sorghum proteins depending on the variety

Author

Sazonova Irina, Bychkova Vera, and Erokhina Anna

Subjects
Microbiology, QR1-502, Physiology, QP1-981, Zoology, QL1-991
Abstract

14 varieties of grain sorghum bred by the Federal State Budgetary Scientific Institution RosNIISK Rossorgo were studied. The amount of protein in grain and its fractions was experimentally determined: albumin, globulins, glutelins and prolamins, a comparative analysis was carried out within each fraction and a conclusion was made about the highest biological value among the studied varieties. The results showed that sorghum seeds contain all four protein fractions, the highest content of which is albumin and glutel. The highest nutritional value was noted in the variety of sorghum Zhemchug, which contained the highest amount of albumin, characterized by a complete amino acid composition, and the lowest content of prolamins, which have a low balance of amino acids. In the varieties Kamelik and Locus, there was an insufficient content of complete proteins that make up the water-soluble and salt-soluble fractions in the grain of these plants. Grain sorghum varieties with the highest amount of protein (Pomegranate, Locus, Pearl and Bachelor) were characterized by a low level of alcohol-soluble protein fraction.

Published
2022
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57. Geodemography of the Saint Petersburg Suburbs

Author

Degusarova V.S., Martynov V.L., and Sazonova I.E.

Subjects
geodemography, suburban area, borders, population, natural population movement, migratory movement, distance, isochrones, Regional economics. Space in economics, HT388
Abstract

In this article, we analyse the structure and the development dynamics of the Saint Petersburg suburbs — home to over 1.6 million people. To this end, we employ statistical, historical, and empirical research methods and carry out a comparative analysis. Geodemographic studies should take into account not only demographic data but also the characteristics of the settlement system. Such studies are particularly important for suburbs. Russian social geography pays little attention to suburban studies, although such territories have become an independent object of research in international geographical science. The Saint Petersburg suburbs are of special interest from the perspective of geodemography, which is explained by the significant size of the area — a result of the territory’s historical development. The formation of the settlement system of the Saint Petersburg suburbs started with the foundation of the city, and continues to this day. Today, their spatial structure is shaped by the current administrative border between Saint Petersburg and the Leningrad region — a product of the territory’s development in the Soviet period of Russia’s history. The lengthy process of border formation has given it a peculiar character. The most vibrant and attractive areas of the suburbs are located at a distance of 14—32 km from the centre of Saint Petersburg, between the isochrones of fortyand ninety-minute transport accessibility. Lying at a distance of approximately 60 km from the city centre, the two-hour travel time band marks the border of both the commuter zone and the Saint Petersburg agglomeration. A new settlement system is emerging within the suburban area of Saint Petersburg — the most economically, demographically, and socially vibrant territory of Russia’s North-West.

Published
2018
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58. ChemInform Abstract: Synthesis and Properties of New Organothiophosphorus Insectoacaricides, Containing N‐Acylated Amino Acid Fragments.

Author

SHIPOV, A. E., primary, GENKINA, G. K., additional, ZHDANOVA, G. V., additional, PETROVSKII, P. V., additional, ROSLAVTSEVA, S. A., additional, SAZONOVA, I. N., additional, SUNDUKOV, O. V., additional, GOLOVKINA, L. S., additional, VOLKOVA, R. I., additional, KAGAN, YU. S., additional, ERSHOVA, E. A., additional, MASTRYUKOVA, T. A., additional, and KABACHNIK, M. I., additional

Published
1995
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60. Isolation, purification, and identification of the virulence protein VirE2 fromAgrobacterium tumefaciens.

Author

Volokhina, I., Sazonova, I., Velikov, V., and Chumakov, M.

Subjects
MICROBIAL virulence, PATHOGENIC microorganisms, MICROBIAL invasiveness, CELLS, GENES, NUCLEIC acids
Abstract

Bacteria of the genusAgrobacteriumare capable of transferring a fragment of their Ti-plasmid T-DNA, in a complex with the proteins VirE2 and VirD2, into the nuclei of plant cells and incorporating it into the chromosome of the host. The mechanisms of T-DNA transportation through the membrane and cytoplasm of the plant cell are unknown. The aim of this work was isolation of the virulence protein VirE2 for studying its role in T-DNA transportation through the membrane and cytoplasm of eukaryotic cells. For VirE2 accumulation, thevirE2gene was cloned into plasmid pQE31. VirE2 was isolated from the cells ofE. colistrain XL1-blue, containing the recombinant plasmid pQE31-virE2. The cells were disrupted ultrasonically, and the protein, with six histidine residues at the N-end, was isolated by means of affinity chromatography on a Ni-NTA-superose column. The purified protein was tested by the immunodot method using polyclonal rabbit antibodies and anti-VirE2 miniantibodies. The ability of the recombinant protein VirE2 to bind to single-stranded DNA was judged from the formation of complexes detected by electrophoresis in agarose gel. Thus, we isolated, purified, and partially characterized theAgrobacterium tumefaciensvirulence protein VirE2, which is capable of binding to single-stranded T-DNA upon transfer to the plant cell. [ABSTRACT FROM AUTHOR]

Published
2005
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61. Современные геодемографические проблемы Евросоюза и миграционный кризис 2010-х годов=Current Geodemographic Problems in the European Union and Migration Crisis of the 2010s

Author

Martynov Vasily L. and Sazonova Irina E.

Subjects
migrant crisis, population, irregular migration routes, Muslim popu­lation of European Union, “no-go zones”, geodemography, миграционный кризис, численность населения, маршру­ты нелегальной миграции, мусульман­ское население Евросоюза, зоны «ноу-гоу», геодемография, International relations, JZ2-6530
Abstract

This article demonstrates the effect of the 2010s migration crisis has had on the demographic development of the European Union. Employing statistics and map­ping, the study argues that the demographic development of EU states is not affected by either socioeconomic performance or religious, linguistic, and cultural charac­teristics. Migration inflow differs significantly by country and EU region. The au­thors analyse major irregular migration routes and show that their use cannot be always explained by using traditional demographic approaches. There is also a dif­ference in the proportion of migrants by country, although settlement systems are very similar. Even with decades spent in the destination country, the Muslim popula­tion remains poorly integrated into the local community and its effect on the latter is insignificant (Turks in Germany). The demographic mosaic of the European Union is becoming increasingly fragmented, which makes any national level demographic forecasts inconsequential. However, community-level forecasts are possible and necessary. All this creates a need for geodemographic research.

Published
2017
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62. Creation of new initial forms of black currant (Ribes nigrum L.) in breeding for adaptation

Author

Sazonov Fedor, Kulikov Ivan, Tumaeva Tatyana, and Sazonova Irina

Subjects
Environmental sciences, GE1-350
Abstract

The article presents the results of studying the black currant varieties collection of the Kokinsky control station of the FSBSI FRC for Horticulture (Bryansk region) for winter hardiness, resistance to Sphaerotheca, and currant gall mite in order to create new initial forms based on the selected genotypes, characterized by a high level of adaptation for their inclusion in the breeding work to improve the crop assortment. The best combinations of crosses (Gamayun × Nara, 33-27-1 × Kipiana, 33-27-1 × Bryanskiy Agat, Favorit × Dar Smolyaninovoy, etc.), the initial forms for inbreeding and free pollination, in the progeny of which the largest number of winter-hardy seedlings, characterized by resistance to Sphaerotheca and gall mite, were identified. Promising winter-hardy Sphaerotheca-resistant forms were identified 4-63-4, 72-03-7, 3-37-26/02, 62-03-7, 1-5-1, 63-35-1, 3-134-01, 28-03-1, 5-82-02, 1-91-01, immune to gall mite.

Published
2021
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63. Legal regulation of the family members’ entrepreneurial activity and inheritance relations: law enforcement problems

Author

Kudryashov Vladislav Vasilyevich, Lepeshkina Valentina Sergeevna, Sazonova Irina Vladimirovna, Potkin Aleksandr Anatolevich, and Altunin Viktor Anatolevich

Subjects
legal regulation, entrepreneurial activity, inheritance relations, family business, Social Sciences
Abstract

The problem of transition in the line of business assets and obligations succession with regard to norms of civil, family and business law represents an important legal problem as for the matter of family business succession. Russian legislation does not determine the family business succession as a single entity, there exists no special regulation as well as the term “entrepreneurial succession”. The doctrine gives a reasonable conclusion that “practices of the recent past reveal substantial problems of marital regimes legal regulation under a digital transformation of the economy”. Inheriting different properties that can be collectively referred to sphere of entrepreneurial activity causes many problems of similar properties transition in the line of succession in the field of law enforcement. Determining particularities of legal regulation of inheritance relations complicated with business activities in order to ensure efficient regulation of succession to business assets and debts and as well to ensure law enforcement stability. The methodological base for the present scientific research is represented by the system of general scientific and specific scientific methods and research techniques, including a historical method, a logical method, a method of system analysis and research, a comparative legal method, a statistical method, a functional-structural method, methods of analysis and synthesis, a method of specification, an empirical and theoretical method, i.e. analogy, deduction. The authors suppose that in conditions of the world financial crisis complicated with consequences of the coronavirus pandemic small businesses are the most vulnerable, including family businesses. The authors believe that a modern lawyer must have systemic knowledge for efficient application of civil law, inheritance law, family law, entrepreneurial law on the basis of the convergence principle in law. The use of a rather broad methodological base allows determining essential properties of legal regulation of the family members’ entrepreneurial activity and inheritance relations from the point of view of law enforcement problems resolution. As for particularities of inheritance regulations application, a joint-stock company is supposed to have certain mechanisms of the protection of its interests in terms of its shares inheritance. For example, it is possible to envisage the right of a private joint-stock company to discourage inclusion within its shareholders a new participant in line with a similar power of the limited liability companies.

Published
2021
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64. Synergy of Business, Law and Economy in the Smart-Contract Implementation

Author

Sazonova Irina V., Mazhaeva Vladlena S., Potkin Alexandr A., and Kuznetsova Marina A.

Subjects
Social Sciences
Abstract

The evolution of digital technologies leads to a tectonic transformation of all spheres of society. Law, as a system of regulating public relations, is changing dynamically along with the development of public relations in different spheres. The development of IT led to the emergence of blockchain technology, which, in turn, became the basis for the development of smart contracts. Smart contract technology, as it develops, causes changes not only in the legislation, but also in the model of interaction between the state and business. Due to smart contracts, a significant part of the rules can be algorithmized, and the regulation can become machine-readable. Purpose of the research: Legal research of the current legislation, the synergy of business, law and economy in the implementation of smart contract technology, determination of theoretical concepts in relation to smart contracts, the content and problems of the application of smart contracts, and identification of the most significant proposals for improving legislation. Methods: The authors of the research used general and specific scientific methods. In the study of the technological foundations of the smart contract, the main methods were analysis, synthesis, analogy, and a system-structural approach.

Published
2021
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65. Artificial Intelligence as Effective Digital Transformation Legal Means of Business, Management, Economy and Technology

Author

Sushkova Olga V., Sazonova Irina V., Tyulin Aleksandr V., and Ruzhentseva Marina S.

Subjects
Social Sciences
Abstract

Currently, entrepreneurial and professional activities are being transformed towards digital business, management, economics and technology, which are viewed through the prism of four new technological areas: artificial intelligence, blockchain, cloud technologies and data analytics. Therefore, we will use multidisciplinary approaches that show how digital tools affect all areas of business processes, management and economics. Purpose of the study: to determine the role and importance of artificial intelligence for building an effective model of business, management, economy and technology transformation, business and professional activities in the context of law and society digital transformation. Methods: The methodological basis of this work was general scientific methods of cognition of legal phenomena, such as synthesis, the method of analogy, formal logic and others, as well as specific scientific methods for studying the category of artificial intelligence in the field of entrepreneurial and professional activity. Results and novelty: To achieve the goals of using AI, it can be described as follows: the first step is to use big data, the second is to apply analytics, and the third is forecasting. AI is used to collect and store data for analysis and further forecasting. Companies in IT, marketing, finance, accounting and sales are using AI to become more competitive and efficient.

Published
2021
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66. Biotransformation of flaxseed meal by effective microorganisms and possibility of application in aquaculture

Author

Sazonova Irina A., Osina Tatyana S., Smutnev Petr V., Zhnichkova Elena G., and Shpul Sergey V.

Subjects
Microbiology, QR1-502, Physiology, QP1-981, Zoology, QL1-991
Abstract

This article describes studies on the impact of effective microorganisms of the “Baikal EM-1” probiotic on the biotransformation of flaxseed meal, its organoleptic and chemical parameters, and changes in amino acid and fatty acid compositions. It has been established that after fermentation in flaxseed meal, the amount of fiber decreases, it saturates with protein, and the amount of essential and non-essential amino acids increases. We made an analysis of the feed value of biotransformed meal depending on the probiotic concentration for the purpose of further use in the feeding of carps – the most effective probiotic concentration for biotransformation was selected. The effect of replacing part of the feed with biotransformed flaxseed meal on the change in liveweight and biochemical parameters of fish blood was studied. Positive dynamics of fishes’ liveweight was noted. The inexpediency of feeding unfermented meal to carps is proved.

Published
2020
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68. Synthesis of new organothiophosphorus insectoacaricides containing N-acylated amino acid fragments.

Author

Shipov, A., Genkina, G., Zhdanova, G., Petrovskii, P., Roslavtseva, S., Sazonova, I., Sundukov, O., Golovkina, L., Volkova, R., Kagan, Yu., Ershova, E., Mastryukova, T., and Kabachnik, M.

Abstract

Methods for the synthesis of O-alkyl S-(N-acyl- N-alkoxycarbonylalkyl)aminomethyl (methyl)thio- and -dithiophosphonates based on the reaction between alkaline-metal salts of O-alkyl (methyl)thio- and -dithiophosphonates and N-alkoxycarbonyl- N-chloromethylglycine or -β-alanine esters and by treatment of O-alkyl (methyl)dithiophosphonoates and N-acylated amino acids or their esters with paraform in the presence of HCl were developed. The compounds obtained exhibit high insectoacaricide activity and selectivity. [ABSTRACT FROM AUTHOR]

Published
1994
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69. The mechanism of a bacterial plasminogen activator intermediate between streptokinase and staphylokinase.

Author

Sazonova, I Y, Houng, A K, Chowdhry, S A, Robinson, B R, Hedstrom, L, and Reed, G L

Abstract

The therapeutic properties of plasminogen activators are dictated by their mechanism of action. Unlike staphylokinase, a single domain protein, streptokinase, a 3-domain (alpha, beta, and gamma) molecule, nonproteolytically activates human (h)-plasminogen and protects plasmin from inactivation by alpha(2)-antiplasmin. Because a streptokinase-like mechanism was hypothesized to require the streptokinase gamma-domain, we examined the mechanism of action of a novel two-domain (alpha,beta) Streptococcus uberis plasminogen activator (SUPA). Under conditions that quench trace plasmin, SUPA nonproteolytically generated an active site in bovine (b)-plasminogen. SUPA also competitively inhibited the inactivation of plasmin by alpha(2)-antiplasmin. Still, the lag phase in active site generation and plasminogen activation by SUPA was at least 5-fold longer than that of streptokinase. Recombinant streptokinase gamma-domain bound to the b-plasminogen.SUPA complex and significantly reduced these lag phases. The SUPA-b.plasmin complex activated b-plasminogen with kinetic parameters comparable to those of streptokinase for h-plasminogen. The SUPA-b.plasmin complex also activated h-plasminogen but with a lower k(cat) (25-fold) and k(cat)/K(m) (7.9-fold) than SK. We conclude that a gamma-domain is not required for a streptokinase-like activation of b-plasminogen. However, the streptokinase gamma-domain enhances the rates of active site formation in b-plasminogen and this enhancing effect may be required for efficient activation of plasminogen from other species.

Published
2001
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71. IR spectra of NO and NO+CO mixtures adsorbed on Fe2O3

Author

Glazneva, G. V., Davydov, A. A., Sazonova, I. S., Shchekochikhin, Yu. M., and Keier, N. P.

Abstract

The interaction of NO and NO+CO mixtures with Fe2O3 has been studied by IR spectroscopy. The conclusion has been made that adsorption bands in the region of 1200–1700 cm−1 belong to nitrite-nitrate structures, the band at 1810 cm−1 characterizes catalytically active particles of NO adsorbed on reduced surface sites, Fe2+, the bands at 2210 and 2240 cm−1 are due to vibrations of gaseous N2O.

Published
1978
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72. Territorial'naja struktura proizvoditel'nyh sil Severo-Zapada Rossii v 2000—2010 godah [The territorial structure of productive forces of Russian North-West in the 2000—2010s]

Author

Martynov Vasily and Sazonova Irina

Subjects
North-West, productive forces, regional development, fixed capital assets, investments, income, standard of living, International relations, JZ2-6530
Abstract

A number of researchers are studying regional specifications of Russia from a variety of different perspectives. Economic geography has developed its own methods of approaching the study of territories, and looks, in particular, at geographical distribution of labour. In this article, we use the framework provided by this discipline to describe the changes in the economy of the Russian North-West in the first decade of the 21st century. We combine publicly available data to propose new methods of evaluation of regional economic efficiency. We also identify and describe new trends of North-West development that have not yet been reflected upon by the researchers in the field. We conclude that, having overcome the crisis of the 1990s, the economy of the Russian North-West displays an overall positive trend of coming back to the territorial structures of the beginning of the 20th century.

Published
2012
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74. Value of the Local and Collective Electronic Properties of Modified TiO2 in Reactions of the Oxidation of Carbon Monoxide and of the Decomposition of Isopropyl Alcohol and Formic Acid

Author

FOREIGN TECHNOLOGY DIV WRIGHT-PATTERSON AFB OHIO, Keer,N. P., Sazonova,I. S., Mikhailova,I. L., Khoklova,T. P., FOREIGN TECHNOLOGY DIV WRIGHT-PATTERSON AFB OHIO, Keer,N. P., Sazonova,I. S., Mikhailova,I. L., and Khoklova,T. P.

Abstract

The report discusses the surface chemistry of titanium dioxide in regards to its catalysis of carbon monoxide oxidation and formic acid and isopropyl alcohol decomposition. The surface electronic structure is the basis of the analysis., Edited machine trans. of mono. Problemy Kinetiki i Kataliza. XV. Mekhanizm i Kinetika Geterogennykh Reaktsii, n.p., 1973 p71-77, by Frank C. Vaughn.

Published
1974

75. THE EFFECT OF ADSORPTION OF GASES AND VAPORS ON THE WORK FUNCTION OF SEMICONDUCTORS WITH THE STRUCTURE OF ZINC BLENDE

Author

FOREIGN TECHNOLOGY DIV WRIGHT-PATTERSON AFB OHIO, Kirovskaya,I. A., Sazonova,I. S., Maidanovskaya,L. G., FOREIGN TECHNOLOGY DIV WRIGHT-PATTERSON AFB OHIO, Kirovskaya,I. A., Sazonova,I. S., and Maidanovskaya,L. G.

Abstract

The work function of an electron from germanium (n-type), Gallium arsenide (n-type) and copper bromide was studied in oxygen, hydrogen, propylene and vapors of isopropyl alcohol at different temperatures. A connection was revealed between the n-type state of the surface of these semiconductors and their adsorption capability with respect to oxygen. (Author), Unedited rough draft trans. of Sibirskii Fiziko-Tekhnicheskii Institut. Trudy (USSR) v43 p380-87 1964.

Published
1966

76. [First experience of application of assist circulation device using biventricular type 'excor']

Author

Gordeev, M. L., Karpenko, M. A., Nikolaev, G. V., Gnevashev, A. S., Malaia, E. I., Naǐmushin, A. V., Rubinchik, V. E., Sukhova, I. V., Mitrofanova, L. B., Somin, M. I., Sazonova, I. V., Stepanov, S. S., and Petr Fedotov

Subjects
трансплантация сердца, chronic cardiac failure, immunosuppression therapy, RD1-811, устройство вспомогательного кровообращения, assist circulation device, Surgery, heart transplantation, хроническая сердечная недостаточность, иммуносупрессивная терапия
Abstract

The frequency of use of assist blood device as the «bridge» to the heart transplantation increased in last years. An assessment of results of first 7 implantations of assist circulation device using biventricular type «Excor» was made. The implantations were performed in Federal Almazov centre of the heart, blood and endocrinology. An observation period after implantation was since 11 till 301 days. The heart transplantation of 4 patients was carried out in different terms after implantation. One of the patients passed away on the fifth day due to the pulmonary embolism. Another patient died on the eleventh day after the implantation because of multiple organ failure against the background of severe chronic cardiac failure. The waiting list of heart transplantation includes 2 patients.

81. HIV testing behaviour and HIV prevalence among female sex workers in Ukraine: findings from an Integrated Bio-Behavioural Survey, 2013-2014.

Author

Tokar A, Sazonova I, Mishra S, Smyrnov P, Saliuk T, Lazarus JV, Broerse JEW, Roura M, Blanchard J, and Becker ML

Subjects
Adolescent, Adult, Female, HIV Infections etiology, HIV Infections prevention & control, Humans, Prevalence, Surveys and Questionnaires, Ukraine epidemiology, Young Adult, HIV Infections epidemiology, Patient Acceptance of Health Care, Risk-Taking, Sex Workers
Abstract

Objectives: Ukraine has one of the largest HIV epidemics in Europe, with high prevalence among female sex workers (FSWs). We aimed to identify factors associated with HIV testing and receipt of the test result in the last 12 months, HIV prevalence and self-reported positive status among FSWs in Ukraine., Methods: We used data from an Integrated Bio-Behavioural Survey among FSWs conducted in 2013-2014. The survey methodology combined three sampling strategies: time and location sampling, respondent-driven sampling and key informant recruitment. We used multivariable regression to identify factors associated with self-reported HIV testing in the last 12 months, HIV prevalence and self-reported positive status among FSWs living with HIV. Explored factors included: age, age at first sex, age at entry into sex work, education, marital status, employment status beside sex work, condom use with last paying or non-paying sexual partner, drug or alcohol consumption and sex work venue., Results: Recent HIV testing was low overall with only 63.2% of FSWs reported having tested and received their test result in the last 12 months prior to the survey. HIV prevalence was 7.1% overall, but only 45.0% of FSWs living with HIV were aware of their HIV status. Testing in the last 12 months with receipt of test result was less common among FSWs who used drugs ever in life (adjusted OR (AOR) 0.7, 95% CI 0.6 to 0.9), women soliciting clients indoors (AOR 0.8, 95% CI 0.7 to 0.9) and those not using a condom with last paying sexual partner (AOR 0.3, 95% CI 0.2 to 0.5). HIV positivity was associated with history of ever using drugs (AOR 2.3, 95% CI 1.4 to 3.6) and soliciting clients outdoors (AOR 1.5, 95% CI 1.1 to 2.0). Women working indoors were less aware of their positive status (AOR 0.1, 95% CI 0.1 to 0.9)., Conclusion: HIV prevalence is high among FSWs in Ukraine, and testing and knowledge of one's status remain insufficient. HIV testing programmes need to expand with strategies to reach specific subgroups of FSWs., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)

Published
2019
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82. Molecular epidemiology reveals the role of war in the spread of HIV in Ukraine.

Author

Vasylyeva TI, Liulchuk M, Friedman SR, Sazonova I, Faria NR, Katzourakis A, Babii N, Scherbinska A, Thézé J, Pybus OG, Smyrnov P, Mbisa JL, Paraskevis D, Hatzakis A, and Magiorkinis G

Subjects
Communicable Disease Control, Epidemics, Female, Geography, HIV Infections complications, HIV-1 genetics, Humans, Likelihood Functions, Male, Phylogeny, Risk-Taking, Sexual Behavior, Substance Abuse, Intravenous complications, Ukraine epidemiology, HIV Infections epidemiology, Molecular Epidemiology, Warfare
Abstract

Ukraine has one of the largest HIV epidemics in Europe, historically driven by people who inject drugs (PWID). The epidemic showed signs of stabilization in 2012, but the recent war in eastern Ukraine may be reigniting virus spread. We investigated the movement of HIV-infected people within Ukraine before and during the conflict. We analyzed HIV-1 subtype-A pol nucleotide sequences sampled during 2012-2015 from 427 patients of 24 regional AIDS centers and used phylogeographic analysis to reconstruct virus movement among different locations in Ukraine. We then tested for correlations between reported PWID behaviors and reconstructed patterns of virus spread. Our analyses suggest that Donetsk and Lugansk, two cities not controlled by the Ukrainian government in eastern Ukraine, were significant exporters of the virus to the rest of the country. Additional analyses showed that viral dissemination within the country changed after 2013. Spearman correlation analysis showed that incoming virus flow was correlated with the number of HIV-infected internally displaced people. Additionally, there was a correlation between more intensive virus movement and locations with a higher proportion of PWID practicing risky sexual behaviors. Our findings suggest that effective prevention responses should involve internally displaced people and people who frequently travel to war-affected regions. Scale-up of harm reduction services for PWID will be an important factor in preventing new local HIV outbreaks in Ukraine., Competing Interests: The authors declare no conflict of interest.

Published
2018
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83. Fibrinolysis is amplified by converting alpha-antiplasmin from a plasmin inhibitor to a substrate.

Author

Sazonova IY, Thomas BM, Gladysheva IP, Houng AK, and Reed GL

Subjects
Amino Acid Sequence, Antibodies, Monoclonal chemistry, Epitopes chemistry, Fibrinolysin chemistry, Humans, Molecular Sequence Data, Plasminogen chemistry, Protein Binding, Protein Conformation, Protein Structure, Tertiary, Recombinant Proteins chemistry, Substrate Specificity, Tissue Plasminogen Activator chemistry, Fibrinolysin antagonists & inhibitors, Fibrinolysis, alpha-2-Antiplasmin biosynthesis
Abstract

alpha(2)-Antiplasmin (alpha(2)-AP) is the fast serpin inhibitor of plasmin and appears to limit the success of treatment for thrombosis. We examined the mechanisms through which monoclonal antibodies (mAbs) against alpha(2)-AP amplify fibrinolysis. The mAbs RWR, 49 and 77 interfered with the ability of alpha(2)-AP to inhibit plasmin, microplasmin and trypsin. In solution, mAbs 49 and 77 bound to alpha(2)-AP with 5-fold to 10-fold higher relative affinity than mAb-RWR, while mAb-RWR bound with greater avidity to immobilized or denatured alpha(2)-AP. Binding studies with chimeric alpha(2)-APs revealed that none of the mAbs bound to sites in alpha(2)-AP that form putative contacts with plasmin, namely the carboxy terminal lysines of alpha(2)-AP, or the reactive center loop in the serpin domain of alpha(2)-AP. Rather, mAb-RWR recognized an epitope in the amino-terminus of alpha(2)-AP (L(13)GNQEPGGQTALKSPPGVCS(32)) near the site at which alpha(2)-AP cross-links to fibrin. mAbs 49 and 77 bound to another conformational epitope in the serpin domain of alpha(2)-AP. mAbs 49 and 77 markedly increased the stoichiometry of plasmin inhibition by alpha(2)-AP (from 1.1 +/- 0.1 to 51 +/- 4 and 67 +/- 7) indicating that they convert alpha(2)-AP from an inhibitor to a substrate of plasmin. This was confirmed by sodium dodecylsulfate polyacrylamide gel electrophoresis analysis showing cleavage of alpha(2)-AP by plasmin in the presence of these mAbs. In summary, these mAbs appear to act at sites distinct from known alpha(2)-AP-plasmin contacts to enhance fibrinolysis by converting alpha(2)-AP from an inhibitor to a plasmin substrate.

Published
2007
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85. [Isolation, purification, and identification of virulence protein VirE2 from Agrobacterium tumefaciens].

Author

Volokhina IV, Sazonova IA, Velikov VA, and Chumakov MI

Subjects
Agrobacterium tumefaciens genetics, Animals, Antibodies immunology, Bacterial Proteins genetics, Bacterial Proteins immunology, Cloning, Molecular, DNA, Single-Stranded chemistry, DNA-Binding Proteins genetics, DNA-Binding Proteins immunology, Electrophoresis, Agar Gel, Ion Channels genetics, Ion Channels immunology, Peptide Library, Plasmids, Rabbits, Recombinant Proteins genetics, Recombinant Proteins immunology, Recombinant Proteins isolation & purification, Virulence, Agrobacterium tumefaciens chemistry, Bacterial Proteins isolation & purification, DNA-Binding Proteins isolation & purification, Ion Channels isolation & purification
Abstract

Bacteria of the genus Agrobacterium are capable of transferring a fragment of their Ti-plasmid, T-DNA, in a complex with the proteins VirE2 and VirD2, into the nuclei of plant cells and incorporating it into the chromosome of the host. The mechanisms of T-DNA transportation through membrane and cytoplasm of the plant cell are unknown. The aim of this work was isolation of virulence protein VirE2 for studying its role in T-DNA transportation through the membrane and cytoplasm of eukaryotic cells. For VirE2 accumulation, virE2 gene was cloned into plasmid pQE31. VirE2 was isolated from the cells of E. coli strain XL1-blue, containing the recombinant plasmid pQE31-virE2. The cells were disrupted ultrasonically, and the protein with six histidine residues at the N-end was isolated by means of affinity chromatography on a Ni-NTA-superose column. The purified protein was tested by the immunodot method using polyclonal rabbit antibodies and anti-VirE2 miniantibodies. The ability of the recombinant protein VirE2 to bind to single-stranded DNA was judged from the formation of complexes detected by electrophoresis in agarose gel. Thus, we isolated, purified, and partially characterized the Agrobacterium tumefaciens virulence protein VirE2 which is capable of binding to single-stranded T-DNA upon transfer to the plant cell.

Published
2005

86. [DNA from various tissues of the Far Eastern larch and its applicability for RAPD assay].

Author

Sazonova IIu, Kozyrenko MM, Artiukova EV, Reunova GD, and Zhuravlev IuN

Subjects
Asia, Eastern, Random Amplified Polymorphic DNA Technique, Siberia, DNA, Plant analysis, Plant Structures chemistry, Trees chemistry
Abstract

We studied applicability of RAPD assay for investigating genetic variability of far eastern larches. Techniques to isolate DNA from larch vegetative organs (needles, young shoots and stems) and megagametophytes were selected, and conditions of polymerase chain reaction were optimized. Fifty out of 84 random primers proved efficient, and 22 and 12 primers initiated weak and no synthesis, respectively. The tested primers worked efficiently on the DNA isolated from both diploid and haploid tissues.

Published
2001

87. Leucine 42 in the fibronectin motif of streptokinase plays a critical role in fibrin-independent plasminogen activation.

Author

Liu L, Sazonova IY, Turner RB, Chowdhry SA, Tsai J, Houng AK, and Reed GL

Subjects
Base Sequence, DNA Primers, Fibronectins chemistry, Kinetics, Recombinant Proteins chemistry, Recombinant Proteins genetics, Recombinant Proteins metabolism, Streptokinase chemistry, Streptokinase genetics, Fibrin metabolism, Fibronectins metabolism, Leucine metabolism, Plasminogen metabolism, Streptokinase metabolism
Abstract

The NH(2) terminus (residues 1-59) of streptokinase (SK) is a molecular switch that permits fibrin-independent plasminogen activation. Targeted mutations were made in recombinant (r) SK1-59 to identify structural interactions required for this process. Mutagenesis established the functional roles of Phe-37and Glu-39, which were projected to interact with microplasmin in the activator complex. Mutation of Leu-42 (rSK1-59(L42A)), a conserved residue in the SK fibronectin motif that lacks interactions with microplasmin, strongly reduced plasminogen activation (k(cat) decreased 50-fold) but not amidolysis (k(cat) decreased 1.5-fold). Otherwise rSK1-59(L42A) and native rSK1-59 were indistinguishable in several parameters. Both displayed saturable and specific binding to Glu-plasminogen or the remaining SK fragment (rSKDelta59). Similarly rSK1-59 and rSK1-59(L42A) bound simultaneously to two different plasminogen molecules, indicating that both plasminogen binding sites were intact. However, when bound to SKDelta59, rSK1-59(L42A) was less effective than rSK1-59 in restructuring the native conformation of the SK A domain, as detected by conformation-dependent monoclonal antibodies. In the light of previous studies, these data provide evidence that SK1-59 contributes to fibrin-independent plasminogen activation through 1) intermolecular interactions with the plasmin in the activator complex, 2) binding interactions with the plasminogen substrate, and 3) intramolecular interactions that structure the A domain of SK for Pg substrate processing.

Published
2000
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88. Kinetic properties of the activator complexes plasmin--staphylokinase and plasmin(ogen)--streptokinase in vitro.

Author

Sazonova IY, Aisina RB, Muhametova LI, and Varfolomeyev SD

Subjects
Dose-Response Relationship, Drug, Esterases chemistry, Fibrinolysis, Humans, Kinetics, Plasminogen Activators chemistry, Time Factors, Fibrinolysin chemistry, Metalloendopeptidases chemistry, Plasminogen chemistry, Streptokinase chemistry
Abstract

Comparative kinetic and electrophoretic study of the interaction of plasminogen (PG) with equimolar concentrations of staphylokinase (SPK) and streptokinase (SK) at 4 and 37 degreesC showed that the PG--SK complex has fibrinolytic and esterase activities, whereas the PG--SPK complex was inactive. Both esterase and fibrinolytic activities were enhanced during the conversion of the PG--SPK complex to the complex of plasmin (PL) with SPK (PL--SPK) at 37 and 4 degreesC, while the PG--SK complex was rapidly converted to the PL--SK complex with higher esterase activity only at 37 degreesC. The catalytic efficiency of Z-Lys-pNP hydrolysis (kcat/Km) by the preformed PL--SPK complex was twofold lower than that in the case of the PL--SK complex. Incubation of the PL--SPK and PG--SK(PL--SK) complexes at 37 degreesC for 24 h was associated with the degradation of the proteins and with different kinetics of lowering of esterase, plasminogen activator, and fibrinolytic activities. The PL--SPK complex was considerably more stable than the PG--SK(PL--SK) complex; streptokinase degraded more rapidly than staphylokinase. Kinetics of lysis of fibrin clots by the two complexes were similar, but the efficiency of lysis of plasma clots by the PL--SPK complex was significantly higher than that in the case of the PG--SK(PL--SK) complex (at 0.03-1 microM). Probably, unlike streptokinase, staphylokinase which is less susceptible to degradation in the PL--SPK complex and is released from the triple complex alpha2-antiplasmin--PL--SPK, forms a potentially highly active new complex with free molecules of plasminogen in the plasma.

Published
1999

89. [Inactivation and stabilization of urokinase fibrinolytic activity in vitro].

Author

Aĭsina RB, Mukhametova LI, Sazonova IIu, and Varfolomeev SD

Subjects
Albumins chemistry, Amidohydrolases pharmacology, Electrophoresis, Polyacrylamide Gel, Enzyme Stability, Fibrinolytic Agents pharmacology, Glycerol chemistry, Humans, Hydrogen-Ion Concentration, Kinetics, Lysine chemistry, Mannitol chemistry, Molecular Weight, Plasminogen Activators pharmacology, Protein Folding, Temperature, Urokinase-Type Plasminogen Activator pharmacology, Amidohydrolases chemistry, Fibrinolytic Agents chemistry, Plasminogen Activators chemistry, Urokinase-Type Plasminogen Activator chemistry
Abstract

When incubated at 30 degrees C and pH 7.4, urokinase lost fibrinolytic activity (i.e., the plasminogen-activating activity measured by the time of fibrin clot lysis) but completely retained amidase activity. The enzyme inactivation rate depended on the urokinase concentration and, at concentrations of more than 1.5 microM, was described by a second order equation, which indicated that the enzyme underwent autolytic degradation (kaut = 3.8 x 10(-3) M-1 min-1). During incubation, urokinase (54 kDa) was converted into its low-molecular-mass form (33 kDa) and products of the A-chain degradation. The amidase activity did not correlate with the fibrinolytic activity in the cases when the enzyme molecule underwent local unfolding or partial degradation. The optimum mixture of agents for stabilizing the fibrinolytic activity of urokinase was found.

Published
1998

90. Substrate and inhibitor specificity of cholinesterases from certain insects.

Author

Brick IL, Brestkin AP, Mandelshtam JE, and Sazonova IN

Subjects
Acetylcholine analogs & derivatives, Animals, Aphids enzymology, Cockroaches enzymology, Ganglia enzymology, Grasshoppers enzymology, In Vitro Techniques, Kinetics, Structure-Activity Relationship, Cholinesterase Inhibitors pharmacology, Cholinesterases metabolism, Insecta enzymology
Published
1975

91. [Bacteriolytic properties of the blood serum of rheumatism patients].

Author

Sazonova IM

Subjects
Adolescent, Adult, Cell Division, Escherichia coli immunology, Humans, In Vitro Techniques, Middle Aged, Staphylococcus aureus immunology, Streptococcus pyogenes immunology, Time Factors, Arthritis, Rheumatoid immunology, Blood Bactericidal Activity, Rheumatic Heart Disease immunology
Published
1980

93. [Cholinesterase of the pea aphid Acyrthosiphon pisi Kalt].

Author

Sazonova IN, Novozhilov KV, Brik IL, and Andreeva NA

Subjects
Animals, Aphids drug effects, Cholinesterase Inhibitors, Insecticides, Organophosphorus Compounds, Aphids enzymology, Cholinesterases metabolism
Published
1972

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