51. Imaging of Structural Plasticity of Dendritic Spines with Two-Photon Microscopy.
- Author
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Saneyoshi T and Hayashi Y
- Subjects
- Animals, Synapses metabolism, Synapses physiology, Mice, Biosensing Techniques methods, Dendritic Spines metabolism, Dendritic Spines ultrastructure, Dendritic Spines physiology, Neuronal Plasticity physiology, Fluorescence Resonance Energy Transfer methods, Microscopy, Fluorescence, Multiphoton methods
- Abstract
Plasticity of synaptic transmission underlies learning and memory. It is accompanied by changes in the density and size of synapses, collectively called structural plasticity. Therefore, understanding the mechanism of structural plasticity is critical for understanding the mechanism of synaptic plasticity. In this chapter, we describe the procedures and equipment required to image structural plasticity of a single dendritic spine, which hosts excitatory synapses in the central nervous system, and underlying molecular interactions/biochemical reactions using two-photon fluorescence lifetime microscopy (2P-FLIM) in combination with Förster resonance energy transfer (FRET)-based biosensors., (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
- Published
- 2024
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