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465 results on '"Rummel, Andreas"'

52. SNARE tagging allows stepwise assembly of a multimodular medicinal toxin

Author

Darios, Frédéric, Niranjan, Dhevahi, Ferrari, Enrico, Zhang, Fan, Soloviev, Mikhail, Rummel, Andreas, Bigalke, Hans, Suckling, Jason, Ushkaryov, Yuri, Naumenko, Nikolay, Shakirzyanova, Anastasia, Giniatullin, Rashid, Maywood, Elizabeth, Hastings, Michael, Binz, Thomas, Davletov, Bazbek, and Südhof, Thomas C.

Published
2010

55. Human-Relevant Sensitivity of iPSC-Derived Human Motor Neurons to BoNT/A1 and B1

Author

Schenke, Maren, Prause, Hélène-Christine, Bergforth, Wiebke, Przykopanski, Adina, Rummel, Andreas, Klawonn, Frank, Seeger, Bettina, and HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.

Subjects
Motor Neurons, Induced Pluripotent Stem Cells, Cell Differentiation, Animal Testing Alternatives, Article, alternative methods, Disease Models, Animal, Mice, Neuroblastoma, in vitro potency determination, Tumor Cells, Cultured, Medicine, motor neurons, Animals, Humans, botulinum neurotoxins, Biological Assay, Botulinum Toxins, Type A, Cells, Cultured
Abstract

The application of botulinum neurotoxins (BoNTs) for medical treatments necessitates a potency quantification of these lethal bacterial toxins, resulting in the use of a large number of test animals. Available alternative methods are limited in their relevance, as they are based on rodent cells or neuroblastoma cell lines or applicable for single toxin serotypes only. Here, human motor neurons (MNs), which are the physiological target of BoNTs, were generated from induced pluripotent stem cells (iPSCs) and compared to the neuroblastoma cell line SiMa, which is often used in cell-based assays for BoNT potency determination. In comparison with the mouse bioassay, human MNs exhibit a superior sensitivity to the BoNT serotypes A1 and B1 at levels that are reflective of human sensitivity. SiMa cells were able to detect BoNT/A1, but with much lower sensitivity than human MNs and appear unsuitable to detect any BoNT/B1 activity. The MNs used for these experiments were generated according to three differentiation protocols, which resulted in distinct sensitivity levels. Molecular parameters such as receptor protein concentration and electrical activity of the MNs were analyzed, but are not predictive for BoNT sensitivity. These results show that human MNs from several sources should be considered in BoNT testing and that human MNs are a physiologically relevant model, which could be used to optimize current BoNT potency testing.

Published
2021

59. Kirchlichkeit der Theologie – wohlverstanden

Author

Metzger, Paul, Rummel, Andreas, Schumacher, Wolfgang, Metzger, P ( Paul ), Rummel, A ( Andreas ), Schumacher, W ( Wolfgang ), Tietz, Christiane, Metzger, Paul, Rummel, Andreas, Schumacher, Wolfgang, Metzger, P ( Paul ), Rummel, A ( Andreas ), Schumacher, W ( Wolfgang ), and Tietz, Christiane

Published
2021

61. Mass transport in shallow turbulent wake flow by planar concentration analysis technique

Author

Carmer, Carl F.v., Rummel, Andreas C., and Jirka, Gerhard H.

Subjects
Hydraulic measurements -- Measurement, Vortex-motion -- Observations, Turbulence -- Measurement, Hydraulic engineering -- Research, Engineering and manufacturing industries, Science and technology
Abstract

A planar concentration analysis (PCA) system is used for observing the transport and mixing of a tracer mass in a shallow turbulent free-surface wake flow of a large cylindrical obstacle. The nonintrusive, fieldwise PCA measuring technique is applied to evaluate depth-averaged mass concentrations by making use of light attenuation due to absorption and scattering processes related to a dissolved tracer mass. The scalar fields are decomposed into a low-frequency quasiperiodic part, the coherent flow, and a randomly fluctuating part. From accompanying near-surface velocity measurements, large-scale coherent structures are identified and related to the coherent mass fields. This allows one to assess the role of the large-scale vortices for advection and diffusion in shallow wake flows. The time--mean wake flow displays a self-similar spanwise distribution both for mass and velocity. The longitudinal development of shallow wakes initially shows the growth of unbounded wakes; in the wake far field an attenuated behavior applies. CE Database subject headings: Shallow water; Mass transport; Turbulent diffusion; Vortices; Flow measurement; Dye.

Published
2009

63. Innovative and Highly Sensitive Detection of Clostridium perfringens Enterotoxin Based on Receptor Interaction and Monoclonal Antibodies

Author

Neumann, Thea, primary, Krüger, Maren, additional, Weisemann, Jasmin, additional, Mahrhold, Stefan, additional, Stern, Daniel, additional, Dorner, Martin B., additional, Feraudet-Tarisse, Cécile, additional, Pöhlmann, Christopher, additional, Schulz, Katharina, additional, Messelhäußer, Ute, additional, Rimek, Dagmar, additional, Gessler, Frank, additional, Elßner, Thomas, additional, Simon, Stéphanie, additional, Rummel, Andreas, additional, and Dorner, Brigitte G., additional

Published
2021
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67. NANOPROBING AT LOW BEAM ENERGY, ADDRESSING CURRENT AND FUTURE NODES.

Author

Rummel, Andreas and Smith, Andrew Jonathan

Subjects
*HIGH resolution imaging
Abstract

The article reports that As the structures in semiconductor devices continue to shrink, analyzing individual transistors using nanoprobing techniques becomes more and more challenging. In many cases, the employed nanoprobing techniques are based on scanning electron microscopy (SEM). However, it has been shown that the incident electron beam can induce changes to a transistor's behavior.

Published
2022
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68. Arg (super)362 and Tyr (super)365 of the botulinum neurotoxin type A light chain are involved in transition state stabilization

Author

Binz, Thomas, Bade, Steffen, Rummel, Andreas, Kollewe, Astrid, and Alves, Jurgen

Subjects
Biochemistry -- Research, Argon -- Physiological aspects, Tyrosine -- Physiological aspects, Neurotoxic agents -- Physiological aspects, Metalloenzymes -- Physiological aspects, Zinc -- Physiological aspects, Biological sciences, Chemistry
Abstract

Research has been conducted on the botulinum neurotoxin type A light chain which acts as zinc endopeptidase. Results suggest that the members of the gluzincin metalloprotease superfamily, Arg (super)362 guanido group and Tyr (super)365 hydroxyl group, accomplish transition state stabilization.

Published
2002

85. Protocol for cryo-focused ion beam lift-out technique

Author

Schaffer, Miroslava, primary, Pfeffer, Stefan, additional, Mahamid, Julia, additional, Kleindiek, Stephan, additional, Laugks, Tim, additional, Albert, Sahradha, additional, Engel, Benjamin D., additional, Rummel, Andreas, additional, Smith, Andrew, additional, Baumeister, Wolfgang, additional, and Plitzko, Juergen M., additional

Published
2019
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94. Historical Perspectives and Guidelines for Botulinum Neurotoxin Subtype Nomenclature

Author

Peck, Michael W., Smith, Theresa J., Anniballi, Fabrizio, Austin, John W., Bano, Luca, Bradshaw, Marite, Cuervo, Paula, Cheng, Luisa W., Derman, Yagmur, Dorner, Brigitte G., Fisher, Audrey, Hill, Karen K., Kalb, Suzanne R., Korkeala, Hannu, Lindström, Miia, Lista, Florigio, Luquez, Carolina, Mazuet, Christelle, Pirazzini, Marco, Popoff, Michel R., Rossetto, Ornella, Rummel, Andreas, Sesardic, Dorothea, Singh, Bal Ram, Stringer, Sandra C., Departments of Faculty of Veterinary Medicine, Food Hygiene and Environmental Health, Hannu Korkeala / Principal Investigator, and Miia Lindström / Principal Investigator

Subjects
botulism, subtypes, AMINO-ACID-SEQUENCE, NONPROTEOLYTIC CLOSTRIDIUM-BOTULINUM, GROUP-I, ANTIBODY-BASED IMMUNOASSAY, INFANT BOTULISM, NUCLEOTIDE-SEQUENCE ANALYSIS, B NEUROTOXIN, FRAGMENT LENGTH POLYMORPHISM, botulinum, Clostridium botulinum, neurotoxins, GENETIC DIVERSITY, nomenclature, guidelines, MONOCLONAL-ANTIBODIES, 1183 Plant biology, microbiology, virology
Abstract

Botulinum neurotoxins are diverse proteins. They are currently represented by at least seven serotypes and more than 40 subtypes. New clostridial strains that produce novel neurotoxin variants are being identified with increasing frequency, which presents challenges when organizing the nomenclature surrounding these neurotoxins. Worldwide, researchers are faced with the possibility that toxins having identical sequences may be given different designations or novel toxins having unique sequences may be given the same designations on publication. In order to minimize these problems, an ad hoc committee consisting of over 20 researchers in the field of botulinum neurotoxin research was convened to discuss the clarification of the issues involved in botulinum neurotoxin nomenclature. This publication presents a historical overview of the issues and provides guidelines for botulinum neurotoxin subtype nomenclature in the future.

Published
2017

100. Toxins 2017 conference in Madrid

Author

Albanese, Alberto, primary, Cardoso, Francisco, additional, Fujinaga, Yukako, additional, Jankovic, Joseph, additional, Rummel, Andreas, additional, and Schiavo, Giampietro, additional

Published
2018
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