Search

Michele Massimino,1,2,* Stefania Stella,2,3,* Elena Tirrò,2,3 Maria Stella Pennisi,2,3 Fabio Stagno,4 Silvia Rita Vitale,2,3 Chiara Romano,2,5 Cristina Tomarchio,2,3 Nunziatina Laura Parrinello,4 Livia Manzella,2,3 Francesco Di Raimondo,4 Paolo Vigneri2,3,6 1Department of Surgery and Medical-Surgical Specialties, University of Catania, Catania, Italy; 2Center of Experimental Oncology and Hematology, A.O.U. Policlinico “G. Rodolico-S. Marco”, Catania, Italy; 3Department of Clinical and Experimental Medicine, University of Catania, Catania, Italy; 4Division of Hematology, A.O.U. Policlinico “G. Rodolico-S. Marco”, Catania, Italy; 5Department of Medical, Surgical Sciences and Advanced Technologies “G.F. Ingrassia”, Anatomic Pathology, University of Catania, Catania, Italy; 6Humanitas Istituto Clinico Catanese, University Oncology Department, Catania, Italy*These authors contributed equally to this workCorrespondence: Michele Massimino, A.U.O. Policlinico G. Rodolico S. Marco, Via Santa Sofia 78, Catania, 95123, Italy, Tel +39-95-3781952, Fax +39-95-3781949, Email michedot@yahoo.itPurpose: Chronic Myeloid Leukemia (CML) is a clonal disorder of the hematopoietic stem cell caused by expression of the BCR::ABL1 oncoprotein. High BCR::ABL1 levels have been associated to proliferative advantage of leukemic cells, blast crisis progression and tyrosine kinase inhibitors (TKIs) inefficacy. We have previously shown that high BCR::ABL1/GUSIS transcripts measured at diagnosis are associated with inferior responses to standard dose Imatinib (IM). However, the mechanisms underlying the higher rates of disease progression and development of TKIs resistance dependent on elevated BCR::ABL1 levels remain unclear.Methods: Leukemic cells were collected from CML patients showing, at diagnosis, high or low BCR::ABL1/GUSIS. BCR::ABL1 expression levels were measured using real-time PCR. Short-term culture and long-term culture-initiating cells assays were

Chaetopteridae forms a monophyletic clade showing an uncertain position within Annelida. The family has 75 ubiquitous species within four genera that cluster in two well-supported clades (Chaetopterus–Mesochaetopterus and Spiochaetopterus–Phyllochaetopterus) and includes several cryptic species complexes. Based on integrative taxonomy and supported by citizen science, here we describe one new and two unnamed species of Caribbean chaetopterids. Partial sequences from the nuclear 18S rRNA and mitochondrial Cytochrome Oxidase I genes of all known chaetopterid genera allowed us to (1) discuss the phylogeny of the family and (2) assign the three species into Mesochaetopterus (two) and Phyllochaetopterus (one). Mesochaetopterus stinapa, sp. nov. clearly diverged from all species of the genus, whereas Mesochaetopterus aff. xerecus forms a separate clade with Mesochaetopterus rogeri (Europe) and Mesochaetopterus xerecus (Brazil). Phyllochaetopterus aff. verrilli forms a separate clade with Phyllochaetopterus arabicus (Red Sea) and the closely related sequences from Hawai’i, Australia and French Polynesia attributed to Phyllochaetopterus verrilli (or cf. verrilli). Despite observing differences in morphology (e.g. palp colour pattern, presence or absence of eyespots, chaetal morphology and arrangement) and biogeographical distributions, only the erection of M. stinapa as a new species is well supported by the genetic distance, barcoding gap and species discrimination analyses. Our results emphasise the existence of cryptic species complexes within Mesochaetopterus and Phyllochaetopterus, whose taxonomy will require further morphological, biogeographical and molecular data to be resolved.

The genus Marphysa (Annelida: Eunicidae) is represented by only three species, Marphysa sanguinea, Marphysa aegypti and Marphysa birgeri, in the Mediterranean Sea. Combining morphological, molecular data (16S rRNA and cytochrome c oxidase subunit I mitochondrial loci) and environmental information, we present the first Mediterranean report of Marphysa chirigota, based on the specimens collected at Radès Station (Gulf of Tunis, W Mediterranean). The current information on species distribution in Marphysa strongly supports that M. sanguinea inhabits hard bottoms and has a restricted distribution close to its type location (south English coast and nearby NE European Atlantic). Radès Station specimens and all those reported as M. sanguinea along the Tunisian coast were found in shallow water soft bottoms. Therefore, we suggest that the presence of M. sanguinea in Tunisia seems doubtful, and all species reports of Marphysa from Tunisia might correspond to M. chirigota.

El turismo en Italia representa una de las mayores fuentes de riqueza del país. Los turistas que visitan Italia proceden de todo el mundo y gracias a los monumentos culturales, la gran historia que caracterizaaeste país siempre despertará el interés de los turistas. Por esto los establecimientos turísticos presentes en Italia aumentan cada año y con tanta oferta es necesario ofrecer un servicio de excelencia para que los turistas elijan un establecimiento antes que otro.El presente trabajo de fin de master tiene como objetivo el análisis de un establecimiento turístico ubicado en Modica, una ciudad de la costa sureste de Sicilia, una de las islas de Italia. Se trata de una Villa antigua que cada verano está alquilada como casa vacacional a turistas y tiene un buen potencial; sin embargo, haría falta mejorar, desde varios puntos de vista, la Villa para poder aumentar las llegadas y atraer a los clientes para que puedan volver. El trabajo está organizado en cuatro capítulos: en el Capítulo 1, como la Villa pertenece a la categoría de casas ubicadas en zonas rurales, vamos a describir el turismo rural, sus principales características y categorías, analizando la demanda y la oferta relativas a esta tipología de turismo. En el Capítulo 2, sigue una descripción detallada del territorio en el que se encuentra la Villa rural, con las potenciales atraccionespara los turistas y, finalmente una descripción del alojamiento, de su historia y de los servicios ofrecidos. El Capítulo 3 pretendellevar a cabo un análisis interno de la situación comercial del alojamiento,analizando los problemas relativos al producto, al precio, a la distribución, a la comunicación online y a la reputación, que han llevado a una disminución de las llegadas de turistas en los últimos años. Finalmente, en el Capítulo 4 se lleva a cabo el plan de mejora a través de propuestas concretas, de una gestión llevada a cabo de manera más profesional para fidelizar los clientes pasados y actuales y al mismo tiemp

Wood boring bivalves of the family Xylophagaidae inhabit sunken wood on the deepsea floor where they play a key role in the degradation of this organic matter in the ocean. The patchiness of wood-fall habitats is impeding targeted sampling and little is therefore known on xylophagaid biology. We investigated for the first time the diversity and biogeography of Xylophagaidae in the NE-Atlantic and the Mediterranean over a broad geographic range and in various water depths using experimental wood deployments. We combined morphological and molecular analyses for species discrimination. A phylogenetic reconstruction based on 18S and 28S rRNA and COI genes revealed non-monophyly of the type genus, Xylophaga Turton (1822), and led us to revise the taxonomy and erect the genus Xylonora gen. nov. COI haplotypes of the most abundant species revealed broad Atlanto-Mediterranean genetic connectivity for Xylophaga dorsalis and Xylonora atlantica new comb., while genetic connectivity appears limited for Abditoconus brava across the entrance of the Mediterranean. We provide the first COI barcode data for Xylophagaidae as a solid base for future taxonomic work. Wood deployments in a broad geographic range provided a powerful tool for research on Xylophagaidae allowing for conclusions on ecological requirements of xylophagaid species.