51. Hormone-sensitive lipase deficiency disturbs the fatty acid composition of mouse testis
- Author
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Óscar Pastor, P. Mariscal, Rebeca Busto, María E. Casado, Alberto Canfrán-Duque, Miguel A. Lasunción, Javier Martínez-Botas, Antonia Martín-Hidalgo, and Fredric B. Kraemer
- Subjects
Fatty Acid Desaturases ,Male ,medicine.medical_specialty ,FADS1 ,Fatty Acid Elongases ,FADS2 ,Clinical Biochemistry ,Plasmalogens ,Gene Expression ,Hormone-sensitive lipase ,Biology ,chemistry.chemical_compound ,Mice ,Essential fatty acid ,Mead acid ,Acetyltransferases ,Internal medicine ,Testis ,medicine ,Animals ,RNA, Messenger ,Spermatogenesis ,Infertility, Male ,chemistry.chemical_classification ,Mice, Knockout ,Fatty acid metabolism ,Fatty Acids, Essential ,Myocardium ,food and beverages ,Fatty acid ,Cell Biology ,Sterol Esterase ,Lipid Metabolism ,Endocrinology ,Biochemistry ,chemistry ,Organ Specificity ,lipids (amino acids, peptides, and proteins) ,sense organs ,Fatty Acid Synthases ,Polyunsaturated fatty acid - Abstract
Hormone-sensitive lipase (HSL) is a key enzyme in the mobilization of fatty acids from intracellular stores. In mice, HSL deficiency results in male sterility caused by a major defect in spermatogenesis. The testes contain high concentrations of PUFA and specific PUFA are essential for spermatogenesis. We investigated the fatty acid composition and the mRNA levels of key enzymes involved in fatty acid metabolism in testis of HSL-knockout mice. HSL deficiency altered fatty acid composition in the testis but not in plasma. The most important changes were decreases in the essential n-6 PUFA LNA and the n-3 PUFA ALA, and an increase in the corresponding synthesis intermediates C22:4n-6 and C22:5n-3 without changes in DPAn-6 or DHA acids. Mead acid, which has been associated with an essential fatty acid deficit leading to male infertility, was increased in the testis from HSL-knockout mice. Moreover, the expression of SCD-1, FADS1, and FADS2 was increased while expression of ELOVL2, an essential enzyme for the formation of very-long PUFA in testis, was decreased. Given the indispensability of these fatty acids for spermatogenesis, the changes in fatty acid metabolism observed in testes from HSL-knockout male mice may underlie the infertility of these animals. more...
- Published
- 2012