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249 results on '"Ramesar, Jai"'

60. Malaria parasites contain two identical copies of an elongation factor 1 alpha gene1Note: Nucleotide sequence data reported in this paper are available in the EMBL, GenBank™ and DDJB databases under the accession numbers AJ224150, AJ224151, AJ224153 and AJ224154.1

Author

Vinkenoog, Rinke, primary, Aparecida Sperança, Márcia, additional, van Breemen, Onno, additional, Ramesar, Jai, additional, Williamson, Donald H, additional, Ross-MacDonald, Petra B, additional, Thomas, Alan W, additional, Janse, Chris J, additional, del Portillo, Hernando A, additional, and Waters, Andrew P, additional

Published
1998
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66. Experimentally controlled downregulation of the histone chaperone FACT in Plasmodium berghei reveals that it is critical to male gamete fertility.

Author

Laurentino, Eliane C., Taylor, Sonya, Mair, Gunnar R., Lasonder, Edwin, Bartfai, Richard, Stunnenberg, Hendrik G., Kroeze, Hans, Ramesar, Jai, Franke-Fayard, Blandine, Khan, Shahid M., Janse, Chris J., and Waters, Andrew P.

Subjects
BIOLOGY experiments, HISTONES, MOLECULAR chaperones, SPERMATOZOA, CHROMATIN, PLASMODIUM, GENE expression, RNA polymerases, GERM cells
Abstract

Summary Human FACT (facilitates chromatin transcription) consists of the proteins SPT16 and SSRP1 and acts as a histone chaperone in the (dis)assembly of nucleosome (and thereby chromatin) structure during transcription and DNA replication. We identified a Plasmodium berghei protein, termed FACT-L, with homology to the SPT16 subunit of FACT. Epitope tagging of FACT-L showed nuclear localization with high expression in the nuclei of (activated) male gametocytes. The gene encoding FACT-L could not be deleted indicating an essential role during blood-stage development. Using a 'promoter-swap' approach whereby the fact-l promoter was replaced by an 'asexual blood stage-specific' promoter that is silent in gametocytes, transcription of fact-l in promoter-swap mutant gametocytes was downregulated compared with wild-type gametocytes. These mutant male gametocytes showed delayed DNA replication and gamete formation. Male gamete fertility was strongly reduced while female gamete fertility was unaffected; residual ookinetes generated oocysts that arrested early in development and failed to enter sporogony. Therefore FACT is critically involved in the formation of fertile male gametes and parasite transmission. 'Promoter swapping' is a powerful approach for the functional analysis of proteins in gametocytes (and beyond) that are essential during asexual blood-stage development. [ABSTRACT FROM AUTHOR]

Published
2011
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67. A Novel 'Gene Insertion/Marker Out' (GIMO) Method for Transgene Expression and Gene Complementation in Rodent Malaria Parasites.

Author

Jing-wen Lin, Annoura, Takeshi, Sajid, Mohammed, Chevalley-Maurel, Séverine, Ramesar, Jai, Klop, Onny, Franke-Fayard, Blandine M. D., Janse, Chris J., and Khan, Shahid M.

Subjects
VIRAL genetics, GENE transfection, GENETIC transformation, PROTOZOAN diseases, PARASITES, MALARIA, COMPLEMENTATION (Genetics)
Abstract

Research on the biology of malaria parasites has greatly benefited from the application of reverse genetic technologies, in particular through the analysis of gene deletion mutants and studies on transgenic parasites that express heterologous or mutated proteins. However, transfection in Plasmodium is limited by the paucity of drug-selectable markers that hampers subsequent genetic modification of the same mutant. We report the development of a novel 'gene insertion/marker out' (GIMO) method for two rodent malaria parasites, which uses negative selection to rapidly generate transgenic mutants ready for subsequent modifications. We have created reference mother lines for both P. berghei ANKA and P. yoelii 17XNL that serve as recipient parasites for GIMO-transfection. Compared to existing protocols GIMO-transfection greatly simplifies and speeds up the generation of mutants expressing heterologous proteins, free of drug-resistance genes, and requires far fewer laboratory animals. In addition we demonstrate that GIMO-transfection is also a simple and fast method for genetic complementation of mutants with a gene deletion or mutation. The implementation of GIMO-transfection procedures should greatly enhance Plasmodium reverse-genetic research. [ABSTRACT FROM AUTHOR]

Published
2011
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68. Malaria parasites lacking eef1a have a normal S/M phase yet grow more slowly due to a longer G1 phase.

Author

Janse, Chris J., Haghparast, Alireza, Speranca, Márcia A., Ramesar, Jai, Kroeze, Hans, del Portillo, Hernando A., and Waters, Andrew P.

Subjects
GROWTH factors, PROTEIN synthesis, MORPHOLOGY, MALARIA, GENES, PLASMODIUM, MOLECULAR biology
Abstract

Eukaryotic elongation factor 1A (eEF1A) plays a central role in protein synthesis, cell growth and morphology. Malaria parasites possess two identical genes encoding eEF1A (e ef1aa and eef1ab). Using pbeef1a– Plasmodium berghei mutants that lack an eEF1a gene, we demonstrate that the level of eEF1A production affects the proliferation of blood stages and parasite fitness. Pbeef1a– parasites can complete the vertebrate and mosquito phases of the life cycle, but the growth phase of the asexual blood stages is extended by up to 20%. Analysis of the cell cycle by flow cytometry as well as transcriptional analyses revealed that the duration of the S and M phases and the number of daughter cells produced were not detectably affected, but that the G1 phase is elongated. Thus, as in budding yeast, a growth threshold must be achieved by blood-stage Plasmodium parasites to permit transition from G1 into S/M phase. Initial analyses indicate that transcriptional events associated with gametocyte development were not remarkably retarded. Insight into protein synthesis and its influence on cell proliferation might be used to generate slow-growing (attenuated) parasites. [ABSTRACT FROM AUTHOR]

Published
2003
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69. Reduced CD36-dependent tissue sequestration of Plasmodium-infected erythrocytes is detrimental to malaria parasite growth in vivo

Author

Fonager, Jannik, Pasini, Erica M., Braks, Joanna A.M., Klop, Onny, Ramesar, Jai, Remarque, Edmond J., Vroegrijk, Irene O.C.M., van Duinen, Sjoerd G., Thomas, Alan W., Khan, Shahid M., Mann, Matthias, Kocken, Clemens H.M., Janse, Chris J., and Franke-Fayard, Blandine M.D.

Abstract

Adherence of parasite-infected red blood cells (irbc) to the vascular endothelium of organs plays a key role in the pathogenesis of Plasmodium falciparum malaria. The prevailing hypothesis of why irbc adhere and sequester in tissues is that this acts as a mechanism of avoiding spleen-mediated clearance. Irbc of the rodent parasite Plasmodium berghei ANKA sequester in a fashion analogous to P. falciparum by adhering to the host receptor CD36. To experimentally determine the significance of sequestration for parasite growth, we generated a mutant P. berghei ANKA parasite with a reduced CD36-mediated adherence. Although the cognate parasite ligand binding to CD36 is unknown, we show that nonsequestering parasites have reduced growth and we provide evidence that in addition to avoiding spleen removal, other factors related to CD36-mediated sequestration are beneficial for parasite growth. These results reveal for the first time the importance of sequestration to a malaria infection, with implications for the development of strategies aimed at reducing pathology by inhibiting tissue sequestration.

Published
2012
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70. Transgenic Expression of a Mosquito-Stage Malarial Protein, Pbs21, in Blood Stages of Transformed Plasmodium bergheiand Induction of an Immune Response upon Infection

Author

Margos, Gabriele, van Dijk, Melissa R., Ramesar, Jai, Janse, Chris J., Waters, Andrew P., and Sinden, Robert E.

Abstract

ABSTRACTPbs21 is a surface protein of the ookinete of Plasmodium berghei, which can induce a potent transmission-blocking immune response. Pbs21 is normally expressed only by parasite stages in the mosquito, i.e., female gametes/zygotes, ookinetes, and oocysts. However, the Pbs21 gene is transcribed in female gametocytes which circulate in the bloodstream of the host, where translation of the resulting mRNA is totally repressed. Episomal transfection has been used to investigate whether expression of Pbs21 protein could be achieved in blood stages of the parasite. By using plasmid pMD221, the complete mRNA-encoding region of Pbs21, flanked only by 218 nucleotides (nt) of its promoter region and 438 nt of its 3′ region downstream from the polyadenylation site, was introduced into the blood stages of gametocyte-producing and non-gametocyte-producing clones of P. berghei. In both of these transformed parasite lines, Pbs21 protein was expressed in asexual trophozoites, schizonts, and, when present, in both male and female gametocytes. Hence, the flanking regions present are sufficient to allow transcription but lack the elements that exert natural control of sex- and stage-specific transcription. The mRNA and the protein expressed by transformed blood stages were indistinguishable from the wild-type forms by the criteria tested, and the protein was recognized by both conformation-dependent and conformation-independent monoclonal antibodies raised against native Pbs21. In mice infected with transformed non-gametocyte-producing parasites, a Pbs21-specific immune response was induced and characterized with respect to isotype (IgG2a/IgG2b) and quantity (11.5 ± 10 μg/ml) of antibody produced. However, as found in previous studies, these antibody levels were insufficient to inhibit development of the parasites in the mosquito. The ability to express mosquito midgut-stage antigens in blood-stage parasites will facilitate further investigations of molecular and immunological properties of these proteins.

Published
1998
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71. Genome-Scale Identification of Essential Metabolic Processes for Targeting the Plasmodium Liver Stage

Author

Stanway, Rebecca R., Bushell, Ellen, Chiappino-Pepe, Anush, Roques, Magali, Sanderson, Theo, Franke-Fayard, Blandine, Caldelari, Reto, Golomingi, Murielle, Nyonda, Mary, Pandey, Vikash, Schwach, Frank, Chevalley, Séverine, Ramesar, Jai, Metcalf, Tom, Herd, Colin, Burda, Paul-Christian, Rayner, Julian C., Soldati-Favre, Dominique, Janse, Chris J., Hatzimanikatis, Vassily, Billker, Oliver, and Heussler, Volker T.

Subjects
570 Life sciences, biology, 3. Good health

72. Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

Author

Fonager, Jannik, Blandine MD Franke-Fayard, Adams, John H, Ramesar, Jai, Onny Klop, Khan, Shahid M, Janse, Chris J, and Waters, Andrew P

Subjects
animal structures, fungi, parasitic diseases, 3. Good health
Abstract

Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

73. Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

Author

Fonager, Jannik, Blandine MD Franke-Fayard, Adams, John H, Ramesar, Jai, Onny Klop, Khan, Shahid M, Janse, Chris J, and Waters, Andrew P

Subjects
animal structures, fungi, parasitic diseases, 3. Good health
Abstract

Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

75. Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

Author

Fonager, Jannik, Blandine MD Franke-Fayard, Adams, John H, Ramesar, Jai, Onny Klop, Khan, Shahid M, Janse, Chris J, and Waters, Andrew P

Subjects
animal structures, fungi, parasitic diseases, 3. Good health
Abstract

Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

76. Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

Author

Fonager, Jannik, Blandine MD Franke-Fayard, Adams, John H, Ramesar, Jai, Onny Klop, Khan, Shahid M, Janse, Chris J, and Waters, Andrew P

Subjects
animal structures, fungi, parasitic diseases, 3. Good health
Abstract

Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

77. Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

Author

Fonager, Jannik, Blandine MD Franke-Fayard, Adams, John H, Ramesar, Jai, Onny Klop, Khan, Shahid M, Janse, Chris J, and Waters, Andrew P

Subjects
animal structures, fungi, parasitic diseases, 3. Good health
Abstract

Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

78. Additional file 5 of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

Author

Fonager, Jannik, Blandine MD Franke-Fayard, Adams, John H, Ramesar, Jai, Onny Klop, Khan, Shahid M, Janse, Chris J, and Waters, Andrew P

Subjects
3. Good health
Abstract

Additional file 5: List of primers used in this study. Left column: primer number. Middle column: Primer sequence. Right column: Description of primer and orientation (F: Forward, R: Reverse). (PDF 63 KB)

80. Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

Author

Fonager, Jannik, Blandine MD Franke-Fayard, Adams, John H, Ramesar, Jai, Onny Klop, Khan, Shahid M, Janse, Chris J, and Waters, Andrew P

Subjects
animal structures, fungi, parasitic diseases, 3. Good health
Abstract

Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

81. MOESM8 of Chimeric Plasmodium falciparum parasites expressing Plasmodium vivax circumsporozoite protein fail to produce salivary gland sporozoites

Author

Catherin Marin-Mogollon, Pul, Fiona, Miyazaki, Shinya, Imai, Takashi, Ramesar, Jai, Salman, Ahmed, Winkel, Beatrice, Othman, Ahmad, Kroeze, Hans, Chevalley-Maurel, Severine, Reyes-Sandoval, Arturo, Roestenberg, Meta, Franke-Fayard, Blandine, Janse, Chris, and Khan, Shahid

Subjects
fungi, parasitic diseases, 3. Good health
Abstract

Additional file 8. Chimeric rodent parasite lines pb-pvcsp(vk210) and pb-pvcsp(vk247) are able to produce salivary gland sporozoites that are infectious to mice. A. Upper panel, left: Light microscope pictures of wild type P. berghei (WT) and pb-pvcsp oocyst at days 7, 10 and 14 after feeding of An. stephensi mosquitoes. Black arrows indicate oocyst with sporozoite formation and white arrows indicate degenerated, vacuolated oocyst without sporozoite formation (scale bar, 10 µm). Upper panel, right, Percentage of degenerated oocyst in An. stephensi mosquitoes (n = 5) at day 14 after feeding (***P =

82. MOESM8 of Chimeric Plasmodium falciparum parasites expressing Plasmodium vivax circumsporozoite protein fail to produce salivary gland sporozoites

Author

Catherin Marin-Mogollon, Pul, Fiona, Miyazaki, Shinya, Imai, Takashi, Ramesar, Jai, Salman, Ahmed, Winkel, Beatrice, Othman, Ahmad, Kroeze, Hans, Chevalley-Maurel, Severine, Reyes-Sandoval, Arturo, Roestenberg, Meta, Franke-Fayard, Blandine, Janse, Chris, and Khan, Shahid

Subjects
fungi, parasitic diseases, 3. Good health
Abstract

Additional file 8. Chimeric rodent parasite lines pb-pvcsp(vk210) and pb-pvcsp(vk247) are able to produce salivary gland sporozoites that are infectious to mice. A. Upper panel, left: Light microscope pictures of wild type P. berghei (WT) and pb-pvcsp oocyst at days 7, 10 and 14 after feeding of An. stephensi mosquitoes. Black arrows indicate oocyst with sporozoite formation and white arrows indicate degenerated, vacuolated oocyst without sporozoite formation (scale bar, 10 µm). Upper panel, right, Percentage of degenerated oocyst in An. stephensi mosquitoes (n = 5) at day 14 after feeding (***P =

83. Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

Author

Fonager, Jannik, Blandine MD Franke-Fayard, Adams, John H, Ramesar, Jai, Onny Klop, Khan, Shahid M, Janse, Chris J, and Waters, Andrew P

Subjects
animal structures, fungi, parasitic diseases, 3. Good health
Abstract

Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

85. Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

Author

Fonager, Jannik, Blandine MD Franke-Fayard, Adams, John H, Ramesar, Jai, Onny Klop, Khan, Shahid M, Janse, Chris J, and Waters, Andrew P

Subjects
animal structures, fungi, parasitic diseases, 3. Good health
Abstract

Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

86. Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

Author

Fonager, Jannik, Blandine MD Franke-Fayard, Adams, John H, Ramesar, Jai, Onny Klop, Khan, Shahid M, Janse, Chris J, and Waters, Andrew P

Subjects
animal structures, fungi, parasitic diseases, 3. Good health
Abstract

Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

88. MOESM4 of Chimeric Plasmodium falciparum parasites expressing Plasmodium vivax circumsporozoite protein fail to produce salivary gland sporozoites

Author

Catherin Marin-Mogollon, Pul, Fiona, Miyazaki, Shinya, Imai, Takashi, Ramesar, Jai, Salman, Ahmed, Winkel, Beatrice, Othman, Ahmad, Kroeze, Hans, Chevalley-Maurel, Severine, Reyes-Sandoval, Arturo, Roestenberg, Meta, Franke-Fayard, Blandine, Janse, Chris, and Khan, Shahid

Subjects
parasitic diseases, 3. Good health
Abstract

Additional file 4. Growth of asexual blood stages and mCherry expression in blood stages. Growth of asexual blood stages of two chimeric P. falciparum parasite lines (pf-pvcsp(vk210) and pf-pvcsp(vk247), a P. falciparum line lacking expression of CSP (PfΔcsp) and P. falciparum wild type (WT) parasites. Parasites of the different cloned lines were cultured in semi-automated culture system for a period of 7 days. Cultures were initiated with a parasitaemia of 0.5%. Arrows indicate the dilution of the cultures with fresh red blood cells to have a final parasitaemia of 1%. B. mCherry-expressing blood stages of PfΔcsp parasites where the csp gene has been disrupted by insertion of an mCherry expression cassette (see Additional file 5 for details of the generation of PfΔcsp). Scale bar, 7 µm.

89. Additional file 5 of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

Author

Fonager, Jannik, Blandine MD Franke-Fayard, Adams, John H, Ramesar, Jai, Onny Klop, Khan, Shahid M, Janse, Chris J, and Waters, Andrew P

Subjects
3. Good health
Abstract

Additional file 5: List of primers used in this study. Left column: primer number. Middle column: Primer sequence. Right column: Description of primer and orientation (F: Forward, R: Reverse). (PDF 63 KB)

91. The malaria secretome: from algorithms to essential function in blood stage infection

Author

Ooij, Christiaan Van, Tamez, Pamela A., Souvik Bhattacharjee, Hiller, Natalia, Harrison, Travis, Liolios, Konstantinos, Kooij, Taco, Ramesar, Jai, Bharath Balu, Adams, John H., Waters, Andrew P., Janse, Chris J., and Haldar, Kasturi

Subjects
FOS: Biological sciences, 69999 Biological Sciences not elsewhere classified, 3. Good health
Abstract

The malaria agent Plasmodium falciparum is predicted to export a "secretome" of several hundred proteins to remodel the host erythrocyte. Prediction of protein export is based on the presence of an ER-type signal sequence and a downstream Host-Targeting (HT) motif (which is similar to, but distinct from, the closely related Plasmodium Export Element [PEXEL]). Previous attempts to determine the entire secretome, using either the HT-motif or the PEXEL, have yielded large sets of proteins, which have not been comprehensively tested. We present here an expanded secretome that is optimized for both P. falciparum signal sequences and the HT-motif. From the most conservative of these three secretome predictions, we identify 11 proteins that are preserved across human- and rodent-infecting Plasmodium species. The conservation of these proteins likely indicates that they perform important functions in the interaction with and remodeling of the host erythrocyte important for all Plasmodium parasites. Using the piggyBac transposition system, we validate their export and find a positive prediction rate of approximately 70%. Even for proteins identified by all secretomes, the positive prediction rate is not likely to exceed approximately 75%. Attempted deletions of the genes encoding the conserved exported proteins were not successful, but additional functional analyses revealed the first conserved secretome function. This gave new insight into mechanisms for the assembly of the parasite-induced tubovesicular network needed for import of nutrients into the infected erythrocyte. Thus, genomic screens combined with functional assays provide unexpected and fundamental insights into host remodeling by this major human pathogen.

92. Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

Author

Fonager, Jannik, Blandine MD Franke-Fayard, Adams, John H, Ramesar, Jai, Onny Klop, Khan, Shahid M, Janse, Chris J, and Waters, Andrew P

Subjects
animal structures, fungi, parasitic diseases, 3. Good health
Abstract

Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

94. Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

Author

Fonager, Jannik, Blandine MD Franke-Fayard, Adams, John H, Ramesar, Jai, Onny Klop, Khan, Shahid M, Janse, Chris J, and Waters, Andrew P

Subjects
animal structures, fungi, parasitic diseases, 3. Good health
Abstract

Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

95. MOESM3 of Chimeric Plasmodium falciparum parasites expressing Plasmodium vivax circumsporozoite protein fail to produce salivary gland sporozoites

Author

Catherin Marin-Mogollon, Pul, Fiona, Miyazaki, Shinya, Imai, Takashi, Ramesar, Jai, Salman, Ahmed, Winkel, Beatrice, Othman, Ahmad, Kroeze, Hans, Chevalley-Maurel, Severine, Reyes-Sandoval, Arturo, Roestenberg, Meta, Franke-Fayard, Blandine, Janse, Chris, and Khan, Shahid

Subjects
parasitic diseases, 3. Good health
Abstract

Additional file 3. Sequence analysis of the P. vivax csp genes introduced into P. falciparum. Long-range PCR fragments (see Fig. 1) were cloned into pET TOPO TA (Invitrogen) and sequenced. See Additional file 2 for primer sequences used for sequencing the complete fragment. A. LR-PCR fragment of parasites of pf-pvcsp(vk210). B. LR-PCR fragment of parasites of pf-pvcsp(vk247).

96. Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

Author

Fonager, Jannik, Blandine MD Franke-Fayard, Adams, John H, Ramesar, Jai, Onny Klop, Khan, Shahid M, Janse, Chris J, and Waters, Andrew P

Subjects
animal structures, fungi, parasitic diseases, 3. Good health
Abstract

Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

97. Additional file 4 of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

Author

Fonager, Jannik, Blandine MD Franke-Fayard, Adams, John H, Ramesar, Jai, Onny Klop, Khan, Shahid M, Janse, Chris J, and Waters, Andrew P

Subjects
3. Good health
Abstract

Additional file 4: Location of intron within the 5'ITR of piggyBac. Green letters: gfp CDS. Black underlined letters: piggyBac 5' ITR. Black underlined bold letters: Intron. (PDF 17 KB)

99. Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

Author

Fonager, Jannik, Blandine MD Franke-Fayard, Adams, John H, Ramesar, Jai, Onny Klop, Khan, Shahid M, Janse, Chris J, and Waters, Andrew P

Subjects
animal structures, fungi, parasitic diseases, 3. Good health
Abstract

Additional file of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

100. Additional file 4 of Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasites

Author

Fonager, Jannik, Blandine MD Franke-Fayard, Adams, John H, Ramesar, Jai, Onny Klop, Khan, Shahid M, Janse, Chris J, and Waters, Andrew P

Subjects
3. Good health
Abstract

Additional file 4: Location of intron within the 5'ITR of piggyBac. Green letters: gfp CDS. Black underlined letters: piggyBac 5' ITR. Black underlined bold letters: Intron. (PDF 17 KB)

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