64 results on '"Rödel, M."'
Search Results
52. Evidence for Crystalline Structure in Dynamically-Compressed Polyethylene up to 200 GPa
- Author
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Hartley, N. J., Brown, S., Cowan, T. E., Cunningham, E., Döppner, T., Falcone, R. W., Fletcher, L. B., Frydrych, S., Galtier, E., Gamboa, E. J., Laso Garcia, A., Gericke, D. O., Glenzer, S. H., Granados, E., Heimann, P. A., Lee, H. J., MacDonald, M. J., MacKinnon, A. J., McBride, E. E., Nam, I., Neumayer, P., Pak, A., Pelka, A., Prencipe, I., Ravasio, A., Rödel, M., Rohatsch, K., Saunders, A. M., Schölmerich, M., Schörner, M., Schuster, A. K., Sun, P., Van Driel, T., Vorberger, J., and Kraus, D.
- Subjects
13. Climate action ,3. Good health - Abstract
Scientific reports 9(1), 4196 (2019). doi:10.1038/s41598-019-40782-5, We investigated the high-pressure behavior of polyethylene (CH2) by probing dynamically-compressed samples with X-ray diffraction. At pressures up to 200 GPa, comparable to those present inside icy giant planets (Uranus, Neptune), shock-compressed polyethylene retains a polymer crystal structure, from which we infer the presence of significant covalent bonding. The A2/m structure which we observe has previously been seen at significantly lower pressures, and the equation of state measured agrees with our findings. This result appears to contrast with recent data from shock-compressed polystyrene (CH) at higher temperatures, which demonstrated demixing and recrystallization into a diamond lattice, implying the breaking of the original chemical bonds. As such chemical processes have significant implications for the structure and energy transfer within ice giants, our results highlight the need for a deeper understanding of the chemistry of high pressure hydrocarbons, and the importance of better constraining planetary temperature profiles., Published by Macmillan Publishers Limited, part of Springer Nature, [London]
53. Specimen collection: an essential tool
- Author
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Prosanta Chakrabarty, Jérôme Fuchs, William N. Eschmeyer, Thomas Valqui, Jorge L. Pérez-Emán, Mason J. Ryan, Harilaos A. Lessios, Frederick H. Sheldon, Michael L. Berumen, D. Ó Foighil, R. Winterbottom, David W. Johnson, Anthony C. Gill, Karsten E. Hartel, Kipling Will, D. L. Mahler, Alexandre Aleixo, Erik Verheyen, David C. Blackburn, Eileen A. Lacey, Gregory Mayer, A. Getahun, M. R. de Carvalho, Terry A. Wheeler, D. R. Robertson, Randall D. Mooi, Carole C. Baldwin, David H. Kavanaugh, Daniel L. Rabosky, Carlos Daniel Cadena, James F. Parham, Christopher C. Witt, Robert P. Guralnick, Lukas Rüber, Kevin Winker, Steven Poe, Rafe M. Brown, Bruce B. Collette, Kent E. Carpenter, Herman L. Mays, Alejandro Pérez-Matus, Maxwell V. L. Barclay, Cody W. Thompson, E. Paul, F. Di Dario, William T. White, Joel Cracraft, Kristofer M. Helgen, Robert C. Drewes, Gary C. Williams, Gary R. Graves, Tomio Iwamoto, James Davis Reimer, Rauri C. K. Bowie, Luiz A. Rocha, Martin F. Gomon, Brian L. Fisher, Jose G. Tello, Michael Vecchione, T. Daniel, Djoko T. Iskandar, K. de Queiroz, Peter C. Wainwright, Mark-Oliver Rödel, Jimmy A. McGuire, Gustav Paulay, John Howard Choat, Helen K. Larson, Jeffrey M. Leis, Chris R. Feldman, Andrew E. Z. Short, Peter D. Roopnarine, Nancy Knowlton, William F. Smith-Vaniz, Jeffrey T. Williams, Luis M. P. Ceríaco, John M. Bates, Federico Bolaños, Victor Mamonekene, Priscilla K. Tucker, Philip Myers, Victor G. Springer, Terrence M. Gosliner, Jon Fjeldså, Mark V. Erdmann, Kendall D. Clements, Matthew J. Miller, Craig Moritz, A. Engilis, Hsuan-Ching Ho, Stan Blum, Gerald R. Allen, John E. McCosker, Ralf Britz, G. Shinohara, Zeehan Jaafar, John P. Dumbacher, Michael W. Nachman, Roy W. McDiarmid, Edward O. Wilson, Warren Brian Simison, John E. Randall, James K. Liebherr, Margaret D. Lowman, Peter W. Fritsch, Ronald A. Nussbaum, Jerry A. Coyne, Francesca Benzoni, Allen Gilbert Collins, Melanie L. J. Stiassny, John J. Pogonoski, Thomas Trnski, Gerardo Chaves, Charles E. Griswold, Keiichi Matsuura, Lynne R. Parenti, Frank Almeda, Aaron M. Bauer, Rich Mooi, Miguel Trefaut Rodrigues, C. M. Berns, Helen F. James, Rocha, L, Aleixo, A, Allen, G, Almeda, F, Baldwin, C, Barclay, M, Bates, J, Bauer, A, Benzoni, F, Berns, C, Berumen, M, Blackburn, D, Blum, S, Bolaños, F, Bowie, R, Britz, R, Brown, R, Cadena, C, Carpenter, K, Ceríaco, L, Chakrabarty, P, Chaves, G, Choat, J, Clements, K, Collette, B, Collins, A, Coyne, J, Cracraft, J, Daniel, T, de Carvalho, M, de Queiroz, K, Di Dario, F, Drewes, R, Dumbacher, J, Engilis Jr., A, Erdmann, M, Eschmeyer, W, Feldman, C, Fisher, B, Fjeldså, J, Fritsch, P, Fuchs, J, Getahun, A, Gill, A, Gomon, M, Gosliner, T, Graves, G, Griswold, C, Guralnick, R, Hartel, K, Helgen, K, Ho, H, Iskandar, D, Iwamoto, T, Jaafar, Z, James, H, Johnson, D, Kavanaugh, D, Knowlton, N, Lacey, E, Larson, H, Last, P, Leis, J, Lessios, H, Liebherr, J, Lowman, M, Mahler, D, Mamonekene, V, Matsuura, K, Mayer, G, Mays Jr., H, Mccosker, J, Mcdiarmid, R, Mcguire, J, Miller, M, Mooi, R, Moritz, C, Myers, P, Nachman, M, Nussbaum, R, Ó Foighil, D, Parenti, L, Parham, J, Paul, E, Paulay, G, Pérez-Emán, J, Pérez-Matus, A, Poe, S, Pogonoski, J, Rabosky, D, Randall, J, Reimer, J, Robertson, D, Rödel, M, Rodrigues, M, Roopnarine, P, Rüber, L, Ryan, M, Sheldon, F, Shinohara, G, Short, A, Simison, W, Smith-Vaniz, W, Springer, V, Stiassny, M, Tello, J, Thompson, C, Trnski, T, Tucker, P, Valqui, T, Vecchione, M, Verheyen, E, Wainwright, P, Wheeler, T, White, W, Will, K, Williams, J, Williams, G, Wilson, E, Winker, K, Winterbottom, R, and Witt, C
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Multidisciplinary ,Specimen collection ,specimen collection ,Biology ,Genealogy ,ZOOGEOGRAFIA ,BIO/05 - ZOOLOGIA - Abstract
Collecting biological specimens for scientific studies came under scrutiny when B. A. Minteer et al. [“Avoiding (re)extinction,” Perspectives, 18 April, p. [260][1]] suggested that this practice plays a significant role in species extinctions. Based on a small number of examples (rare birds
- Published
- 2014
54. Site-selective functionalization of in-plane nanoelectrode-antennas.
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Ochs M, Jucker L, Rödel M, Emmerling M, Kullock R, Pflaum J, Mayor M, and Hecht B
- Abstract
Stacked organic optoelectronic devices make use of electrode materials with different work functions, leading to efficient large area light emission. In contrast, lateral electrode arrangements offer the possibility to be shaped as resonant optical antennas, radiating light from subwavelength volumes. However, tailoring electronic interface properties of laterally arranged electrodes with nanoscale gaps - to e.g. optimize charge-carrier injection - is rather challenging, yet crucial for further development of highly efficient nanolight sources. Here, we demonstrate site-selective functionalization of laterally arranged micro- and nanoelectrodes by means of different self-assembled monolayers. Upon applying an electric potential across nanoscale gaps, surface-bound molecules are removed selectively from specific electrodes by oxidative desorption. Kelvin-probe force microscopy as well as photoluminescence measurements are employed to verify the success of our approach. Moreover, we obtain asymmetric current-voltage characteristics for metal-organic devices in which just one of the electrodes is coated with 1-octadecanethiol; further demonstrating the potential to tune interface properties of nanoscale objects. Our technique paves the way for laterally arranged optoelectronic devices based on selectively engineered nanoscale interfaces and in principle enables molecular assembly with defined orientation in metallic nano-gaps.
- Published
- 2023
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55. Mechano-Stimulus and Environment-Dependent Circularly Polarized TADF in Chiral Copper(I) Complexes and Their Application in OLEDs.
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Muthig AMT, Mrózek O, Ferschke T, Rödel M, Ewald B, Kuhnt J, Lenczyk C, Pflaum J, and Steffen A
- Abstract
Molecular emitters that combine circularly polarized luminescence (CPL) and high radiative rate constants of the triplet exciton decay are highly attractive for electroluminescent devices (OLEDs) or next-generation photonic applications, such as spintronics, quantum computing, cryptography, or sensors. However, the design of such emitters is a major challenge because the criteria for enhancing these two properties are mutually exclusive. In this contribution, we show that enantiomerically pure {Cu(Cbz
R )[( S / R )-BINAP]} [R = H ( 1 ), 3,6- t Bu ( 2 )] are efficient thermally activated delayed fluorescence (TADF) emitters with high radiative rate constants of kTADF up to 3.1 × 105 s-1 from1/3 LLCT states according to our temperature-dependent time-resolved luminescence studies. The efficiency of the TADF process and emission wavelengths are highly sensitive to environmental hydrogen bonding of the ligands, which can be disrupted by grinding of the crystalline materials. The origin of this pronounced mechano-stimulus photophysical behavior is a thermal equilibrium between the1/3 LLCT states and a3 LC state of the BINAP ligand, which depends on the relative energetic order of the excited states and is prone to inter-ligand C-H···π interactions. The copper(I) complexes are also efficient CPL emitters displaying exceptional dissymmetry values glum of up to ±0.6 × 10-2 in THF solution and ±2.1 × 10-2 in the solid state. Importantly for application in electroluminescence devices, the C-H···π interactions can also be disrupted by employing sterically bulky matrices. Accordingly, we have investigated various matrix materials for successful implementation of the chiral copper(I) TADF emitters in proof-of-concept CP-OLEDs.- Published
- 2023
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56. Diamond formation kinetics in shock-compressed C─H─O samples recorded by small-angle x-ray scattering and x-ray diffraction.
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He Z, Rödel M, Lütgert J, Bergermann A, Bethkenhagen M, Chekrygina D, Cowan TE, Descamps A, French M, Galtier E, Gleason AE, Glenn GD, Glenzer SH, Inubushi Y, Hartley NJ, Hernandez JA, Heuser B, Humphries OS, Kamimura N, Katagiri K, Khaghani D, Lee HJ, McBride EE, Miyanishi K, Nagler B, Ofori-Okai B, Ozaki N, Pandolfi S, Qu C, Ranjan D, Redmer R, Schoenwaelder C, Schuster AK, Stevenson MG, Sueda K, Togashi T, Vinci T, Voigt K, Vorberger J, Yabashi M, Yabuuchi T, Zinta LMV, Ravasio A, and Kraus D
- Abstract
Extreme conditions inside ice giants such as Uranus and Neptune can result in peculiar chemistry and structural transitions, e.g., the precipitation of diamonds or superionic water, as so far experimentally observed only for pure C─H and H
2 O systems, respectively. Here, we investigate a stoichiometric mixture of C and H2 O by shock-compressing polyethylene terephthalate (PET) plastics and performing in situ x-ray probing. We observe diamond formation at pressures between 72 ± 7 and 125 ± 13 GPa at temperatures ranging from ~3500 to ~6000 K. Combining x-ray diffraction and small-angle x-ray scattering, we access the kinetics of this exotic reaction. The observed demixing of C and H2 O suggests that diamond precipitation inside the ice giants is enhanced by oxygen, which can lead to isolated water and thus the formation of superionic structures relevant to the planets' magnetic fields. Moreover, our measurements indicate a way of producing nanodiamonds by simple laser-driven shock compression of cheap PET plastics.- Published
- 2022
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57. Dual setting brushite-gelatin cement with increased ductility and sustained drug release.
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Rödel M, Teßmar J, Groll J, and Gbureck U
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- Bone Cements, Compressive Strength, Drug Liberation, Materials Testing, Calcium Phosphates, Gelatin
- Abstract
A novel dual setting brushite-gelatin cement was achieved by genip ininitiated cross-linking of gelatin during cement setting. Although the combination of an inorganic and organic phase resulted in a decrease of the compressive strength from about 10 MPa without polymeric phase to 3-6-MPa for gelatin modified composites, an increase in elastic properties due to the gelatin hydrogel with a concentration of 10.0 w/v% was achieved. For a powder-to-liquid ratio of 2.5 g*mL
-1 , a shift of initial maximum stress value during compression testing was observed up to 5% deformation and tested samples showed a pseudo-ductile fracture behavior. The obtained composites of the different formulations were characterized regarding phase composition, porosity as well as drug loading capacity with rifampicin and vancomycin. For the latter, a sustained and prolonged release was realized with a drug release profile according to the Higuchi model and a release exponent of n = 0.5 for the formulation with a PLR of 2.5 g*mL-1 and an incorporation of 10.0 w/v% gelatin.- Published
- 2022
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58. Color-Switchable Subwavelength Organic Light-Emitting Antennas.
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Grimm P, Zeißner S, Rödel M, Wiegand S, Hammer S, Emmerling M, Schatz E, Kullock R, Pflaum J, and Hecht B
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Future photonic devices require efficient, multifunctional, electrically driven light sources with directional emission properties and subwavelength dimensions. Electrically driven plasmonic nanoantennas have been demonstrated as enabling technology. Here, we present the concept of a nanoscale organic light-emitting antenna (OLEA) as a color- and directionality-switchable point source. The device consists of laterally arranged electrically contacted gold nanoantennas with their gap filled by the organic semiconductor zinc phthalocyanine (ZnPc). Since ZnPc shows preferred hole conduction in combination with gold, the recombination zone relocates depending on the polarity of the applied voltage and couples selectively to either of the two antennas. Thereby, the emission characteristics of the device also depend on polarity. Contrary to large-area OLEDs where recombination at metal contacts significantly contributes to losses, our ultracompact OLEA structures facilitate efficient radiation into the far-field rendering transparent electrodes obsolete. We envision OLEA structures to serve as wavelength-scale pixels with tunable color and directionality for advanced display applications.
- Published
- 2022
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59. Use of IFNγ/IL10 Ratio for Stratification of Hydrocortisone Therapy in Patients With Septic Shock.
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König R, Kolte A, Ahlers O, Oswald M, Krauss V, Roell D, Sommerfeld O, Dimopoulos G, Tsangaris I, Antoniadou E, Jaishankar N, Bogatsch H, Löffler M, Rödel M, Garcia-Moreno M, Tuchscherr L, Sprung CL, Singer M, Brunkhorst F, Oppert M, Gerlach H, Claus RA, Coldewey SM, Briegel J, Giamarellos-Bourboulis EJ, Keh D, and Bauer M
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- Adult, Aged, Animals, Anti-Inflammatory Agents administration & dosage, Anti-Inflammatory Agents adverse effects, Biomarkers, Clinical Decision-Making, Disease Management, Disease Models, Animal, Female, Hemodynamics, Humans, Hydrocortisone administration & dosage, Hydrocortisone adverse effects, Lactic Acid blood, Male, Mice, Middle Aged, Norepinephrine, Odds Ratio, Prognosis, Propensity Score, Shock, Septic diagnosis, Shock, Septic mortality, Treatment Outcome, Anti-Inflammatory Agents therapeutic use, Hydrocortisone therapeutic use, Interferon-gamma blood, Interleukin-10 blood, Shock, Septic blood, Shock, Septic drug therapy
- Abstract
Large clinical trials testing hydrocortisone therapy in septic shock have produced conflicting results. Subgroups may benefit of hydrocortisone treatment depending on their individual immune response. We performed an exploratory analysis of the database from the international randomized controlled clinical trial Corticosteroid Therapy of Septic Shock (CORTICUS) employing machine learning to a panel of 137 variables collected from the Berlin subcohort comprising 83 patients including demographic and clinical measures, organ failure scores, leukocyte counts and levels of circulating cytokines. The identified theranostic marker was validated against data from a cohort of the Hellenic Sepsis Study Group (HSSG) ( n = 246), patients enrolled in the clinical trial of Sodium Selenite and Procalcitonin Guided Antimicrobial Therapy in Severe Sepsis (SISPCT, n = 118), and another, smaller clinical trial (Crossover study, n = 20). In addition, in vitro blood culture experiments and in vivo experiments in mouse models were performed to assess biological plausibility. A low serum IFNγ/IL10 ratio predicted increased survival in the hydrocortisone group whereas a high ratio predicted better survival in the placebo group. Using this marker for a decision rule, we applied it to three validation sets and observed the same trend. Experimental studies in vitro revealed that IFNγ/IL10 was negatively associated with the load of (heat inactivated) pathogens in spiked human blood and in septic mouse models. Accordingly, an in silico analysis of published IFNγ and IL10 values in bacteremic and non-bacteremic patients with the Systemic Inflammatory Response Syndrome supported this association between the ratio and pathogen burden. We propose IFNγ/IL10 as a molecular marker supporting the decision to administer hydrocortisone to patients in septic shock. Prospective clinical studies are necessary and standard operating procedures need to be implemented, particularly to define a generic threshold. If confirmed, IFNγ/IL10 may become a suitable theranostic marker for an urging clinical need., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 König, Kolte, Ahlers, Oswald, Krauss, Roell, Sommerfeld, Dimopoulos, Tsangaris, Antoniadou, Jaishankar, Bogatsch, Löffler, Rödel, Garcia-Moreno, Tuchscherr, Sprung, Singer, Brunkhorst, Oppert, Gerlach, Claus, Coldewey, Briegel, Giamarellos-Bourboulis, Keh and Bauer.)
- Published
- 2021
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60. Tough and Elastic α-Tricalcium Phosphate Cement Composites with Degradable PEG-Based Cross-Linker.
- Author
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Rödel M, Teßmar J, Groll J, and Gbureck U
- Abstract
Dual setting cements composed of an in situ forming hydrogel and a reactive mineral phase combine high compressive strength of the cement with sufficient ductility and bending strength of the polymeric network. Previous studies were focused on the modification with non-degradable hydrogels based on 2-hydroxyethyl methacrylate (HEMA). Here, we describe the synthesis of suitable triblock degradable poly(ethylene glycol)-poly(lactide) (PEG-PLLA) cross-linker to improve the resorption capacity of such composites. A study with four different formulations was established. As reference, pure hydroxyapatite (HA) cements and composites with 40 wt% HEMA in the liquid cement phase were produced. Furthermore, HEMA was modified with 10 wt% of PEG-PLLA cross-linker or a test series containing only 25% cross-linker was chosen for composites with a fully degradable polymeric phase. Hence, we developed suitable systems with increased elasticity and 5⁻6 times higher toughness values in comparison to pure inorganic cement matrix. Furthermore, conversion rate from α-tricalcium phosphate (α-TCP) to HA was still about 90% for all composite formulations, whereas crystal size decreased. Based on this material development and advancement for a dual setting system, we managed to overcome the drawback of brittleness for pure calcium phosphate cements.
- Published
- 2018
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61. Highly flexible and degradable dual setting systems based on PEG-hydrogels and brushite cement.
- Author
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Rödel M, Teßmar J, Groll J, and Gbureck U
- Subjects
- Biocompatible Materials chemistry, Compressive Strength, Elastic Modulus, Hydrogen-Ion Concentration, Materials Testing, Methacrylates chemical synthesis, Polyethylene Glycols chemical synthesis, Proton Magnetic Resonance Spectroscopy, Solutions, Spectroscopy, Fourier Transform Infrared, Stress, Mechanical, X-Ray Diffraction, Bone Cements chemistry, Calcium Phosphates chemistry, Hydrogels chemistry, Methacrylates chemistry, Polyethylene Glycols chemistry
- Abstract
With respect to the composition of natural bone, we established a degradable dual setting system of different poly(ethylene glycol) (PEG)-based hydrogels combined with a brushite cement. The idea was to reinforce the inorganic calcium phosphate mineral phase with an organic, polymeric phase to alter the cement's properties towards ductility and elasticity. Extremely flexible samples were produced via this dual setting approach with a fully reversible elasticity of the samples containing high molecular weight PEG-based hydrogel precursors. Using the decalcifying agent EDTA, the whole inorganic phase was dissolved due to Ca
2+ -complexation and dimensionally stable hydrogels were obtained, indicating a homogenous polymeric phase within the composites. This was also confirmed by SEM-analysis, where no discontinuities or agglomerations of the phase were observed. Additional XRD-measurements proved a significant influence of the coherent polymeric matrix on the conversion from β-TCP/MCPA to brushite with a decrease in signal intensity. The results confirmed a parallelly running process of setting reaction and gelation without an inhibition of the conversion to brushite and the formation of interpenetrating networks of hydrogel and cement. The strengths of this newly developed dual setting system are based on the material degradability as well as flexibility, which can be a promising tool for bone regeneration applications in non-load bearing craniomaxillofacial defects., Statement of Significance: Brushite based calcium phosphate cements (CPCs) are known as bone replacement materials, which degrade in vivo and are replaced by native bone. However, the pure inorganic material shows a brittle fracture behavior. Here, the addition of a polymeric phase can influence the mechanical properties to create more ductile and flexible materials. This polymeric phase should ideally form during cement setting by a polymerization reaction to achieve high polymer loads without altering cement viscosity and it should be degradable in vivo similar to the cement itself. Therefore, we developed a dual setting system based on simultaneous cement setting of brushite and lactide modified poly(ethylene glycol) dimethacrylate (PEG-PLLA-DMA)-based hydrogel. It was evident that the gels form a continuous phase within the cement after radical polymerization with a strong reduction of cement brittleness., (Copyright © 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.)- Published
- 2018
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62. Simultaneous structuring and mineralization of silk fibroin scaffolds.
- Author
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Rödel M, Baumann K, Groll J, and Gbureck U
- Abstract
Silk fibroin is commonly used as scaffold material for tissue engineering applications. In combination with a mineralization with different calcium phosphate phases, it can also be applied as material for bone regeneration. Here, we present a study which was performed to produce mineralized silk fibroin scaffolds with controlled macroporosity. In contrast to former studies, our approach focused on a simultaneous gelation and mineralization of silk fibroin by immersion of frozen silk fibroin monoliths in acidic calcium phosphate solutions. This was achieved by thawing frozen silk fibroin monoliths in acidic calcium phosphate solution, leading to the precipitation of monocalcium phosphate within the silk fibroin matrix. In the second approach, a conversion of incorporated β-tricalcium phosphate particles into brushite was successfully achieved. Furthermore, a controlled cryostructuring process of silk fibroin scaffolds was carried out leading to the formation of parallel-oriented pores with diameters of 30-50 µm., Competing Interests: Declaration of conflicting interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
- Published
- 2018
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63. Batrachochytrium dendrobatidis in amphibians of Cameroon, including first records for caecilians.
- Author
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Doherty-Bone TM, Gonwouo NL, Hirschfeld M, Ohst T, Weldon C, Perkins M, Kouete MT, Browne RK, Loader SP, Gower DJ, Wilkinson MW, Rödel MO, Penner J, Barej MF, Schmitz A, Plötner J, and Cunningham AA
- Subjects
- Animals, Cameroon epidemiology, Mycoses epidemiology, Mycoses microbiology, Population Surveillance, Amphibians, Chytridiomycota isolation & purification, Mycoses veterinary
- Abstract
Amphibian chytrid fungus Batrachochytrium dendrobatidis (Bd) has been hypothesised to be an indigenous parasite of African amphibians. In Cameroon, however, previous surveys in one region (in the northwest) failed to detect this pathogen, despite the earliest African Bd having been recorded from a frog in eastern Cameroon, plus one recent record in the far southeast. To reconcile these contrasting results, we present survey data from 12 localities across 6 regions of Cameroon from anurans (n = 1052) and caecilians (n = 85) of ca. 108 species. Bd was detected in 124 amphibian hosts at 7 localities, including Mt. Oku, Mt. Cameroon, Mt. Manengouba and lowland localities in the centre and west of the country. None of the hosts were observed dead or dying. Infected amphibian hosts were not detected in other localities in the south and eastern rainforest belt. Infection occurred in both anurans and caecilians, making this the first reported case of infection in the latter order (Gymnophiona) of amphibians. There was no significant difference between prevalence and infection intensity in frogs and caecilians. We highlight the importance of taking into account the inhibition of diagnostic qPCR in studies on Bd, based on all Bd-positive hosts being undetected when screened without bovine serum albumin in the qPCR mix. The status of Bd as an indigenous, cosmopolitan amphibian parasite in Africa, including Cameroon, is supported by this work. Isolating and sequencing strains of Bd from Cameroon should now be a priority. Longitudinal host population monitoring will be required to determine the effects, if any, of the infection on amphibians in Cameroon.
- Published
- 2013
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64. CYP35: xenobiotically induced gene expression in the nematode Caenorhabditis elegans.
- Author
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Menzel R, Rödel M, Kulas J, and Steinberg CE
- Subjects
- 2-Pyridinylmethylsulfinylbenzimidazoles, Animals, Atrazine toxicity, Caenorhabditis elegans drug effects, Fluorenes toxicity, Lansoprazole, Omeprazole toxicity, Polychlorinated Biphenyls toxicity, Caenorhabditis elegans physiology, Caenorhabditis elegans Proteins metabolism, Cytochrome P-450 Enzyme System metabolism, Gene Expression Regulation, Enzymologic drug effects, Omeprazole analogs & derivatives, Reproduction drug effects, Reproduction physiology, Xenobiotics toxicity
- Abstract
Although over 80 cytochrome P450 (CYP) encoding genes have been identified in the genome of the nematode Caenorhabditis elegans very little is known about their involvement in biotransformation. This paper demonstrates a concentration-dependent relationship of C. elegans CYP35A1, A2, A5, and C1 gene expression in response to four organic xenobiotics, namely atrazine, PCB52, fluoranthene, and lansoprazole. The toxicity of these xenobiotics was determined using a reproduction assay. CYP-specific messenger RNA expression was analyzed by semi-quantitative RT-PCR resulting in a strongly increasing, concentration-dependent induction well below the EC50 for reproduction. For PCB52, approximately 0.5% of the EC50 induces a 2-fold increase of CYP35 gene expression. Using a double mutant and multiple RNAi of CYP35A/C it was possible to diminish the reproduction decline caused by PCB52 and fluoranthene.
- Published
- 2005
- Full Text
- View/download PDF
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