82 results on '"Quan-Cai, Cui"'
Search Results
52. [Proteomics study of papillary thyroid carcinoma]
- Author
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Jie, Shi, Zhao-Hui, Lu, and Quan-Cai, Cui
- Subjects
Adult ,Male ,Proteomics ,Thyroid Gland ,Down-Regulation ,Middle Aged ,Carcinoma, Papillary ,Neoplasm Proteins ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,Young Adult ,Biomarkers, Tumor ,Humans ,Female ,Thyroid Neoplasms ,Aged - Abstract
To study the proteomics of papillary thyroid carcinoma (PTC), and to detect the differential expression of proteins compared to normal thyroid (NT) tissue.Protein extracts of papillary thyroid carcinoma and normal thyroid tissues from 10 patients were separated by proteomics technique and identified using Image Master software. The selected differential proteins were sent to Proteomics Research Center of Chinese Academy of Medical Sciences for mass-spectrometry identification.The basic 2-dimensional electrophoresis (2-DE) images of PTC and NT were obtained, and four potential differential expression spots were selected for mass-spectrometry identification. Among them, annexin I, peroxiredoxin I, mitochondrial aconitase were up-regulated while carbonic anhydrase I was down-regulated.Proteomics may provide a new direction to study PTC, by identifying some tumor-specific markers, leading to elucidation of the mechanism of tumorigenesis and identification of therapeutic targets.
- Published
- 2008
53. [Proteomics and its applications in the research of papillary thyroid carcinoma]
- Author
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Jie, Shi, Zhao-hui, Lu, and Quan-cai, Cui
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Proteomics ,Repressor Proteins ,Prohibitins ,S100 Proteins ,Biomarkers, Tumor ,HSP27 Heat-Shock Proteins ,Humans ,Cell Cycle Proteins ,Thyroid Neoplasms ,Carcinoma, Papillary ,Serpins ,Cathepsin B ,S100 Calcium Binding Protein A6 - Published
- 2008
54. [Laryngeal pleomorphic rhabdomyosarcoma: report of a case]
- Author
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Jian-ping, Liu and Quan-cai, Cui
- Subjects
Adult ,Diagnosis, Differential ,Leiomyosarcoma ,Male ,Rhabdomyosarcoma ,Humans ,Vimentin ,Laryngectomy ,Histiocytoma, Malignant Fibrous ,Tomography, X-Ray Computed ,Laryngeal Neoplasms - Published
- 2007
55. [Annexin A2 and pathogenesis of cancer: an update]
- Author
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Ling, Zhao and Quan-Cai, Cui
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DNA Replication ,Neoplasms ,Tissue Plasminogen Activator ,Animals ,Humans ,Neoplasm Invasiveness ,Neoplasm Metastasis ,Prognosis ,Annexin A2 ,Endocytosis ,Exocytosis - Published
- 2007
56. [Clinical characters and prognosis of postpartum choriocarcinoma]
- Author
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Cheng-Zhi, Lei, Yang, Xiang, Xi-Run, Wan, Jun-Jun, Yang, Quan-Cai, Cui, Feng-Zhi, Feng, and Xiu-Yu, Yang
- Subjects
Adult ,Postpartum Period ,Middle Aged ,Hysterectomy ,Prognosis ,Combined Modality Therapy ,Treatment Outcome ,Drug Resistance, Neoplasm ,Antineoplastic Combined Chemotherapy Protocols ,Uterine Neoplasms ,Dactinomycin ,Humans ,Chorionic Gonadotropin, beta Subunit, Human ,Female ,Choriocarcinoma ,Fluorouracil ,Retrospective Studies - Abstract
To analyze the clinical characters and prognosis of postpartum choriocarcinoma.Retrospective analysis of 103 patients diagnosed with postpartum choriocarcinoma in Peking Union Medical College Hospital between 1985 and 2005 was performed.In a total of 103 cases of postpartum choriocarcinoma treated with multiagent cytotoxic chemotherapy and combined modality therapy, 92 patients (89.3%) achieved a complete remission while 2 patients had a partial remission, and 9 patients died. Regarding 25 cases with chemorefractory choriocarcinoma and 4 relapsed cases, 18 and 3 cases achieved a complete remission respectively. According to the new International Federation of Gynecology and Obstetrics (FIGO) 2000 staging and risk factor scoring system, 17 cases were at FIGO stage I, 8 cases at stage II, 56 cases at stage III, and 22 cases at stage IV. Complete remission was achieved in 100%, 8/8, 95% and 64% respectively. Thirty-five cases in low risk group had FIGO scoresor = 6, and all achieved a complete remission. Of 68 cases in high risk group with FIGO scoresor = 7, 57 (84%) of them obtained a complete remission. There were significant differences in the two groups (P0.05).Postpartum choriocarcinoma could have an increasing probability of cure with early diagnosis and the use of modern multiagent cytotoxic chemotherapy and combined modality therapy.
- Published
- 2007
57. Clinical and pathologic characteristics and prognosis of placental site trophoblastic tumor
- Author
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Jun, Zhao, Yang, Xiang, Xi Run, Wan, Quan Cai, Cui, and Xiu Yu, Yang
- Subjects
Adult ,Trophoblastic Tumor, Placental Site ,Pregnancy ,Uterine Neoplasms ,Humans ,Female ,Middle Aged ,Prognosis ,Chorionic Gonadotropin - Abstract
To analyze the clinical and pathologic characteristics of placental site trophoblastic tumor (PSTT) cases and to discuss the diagnosis, treatment and prognosis of PSTT.The clinical and pathologic data on 11 patients with PSTT at Peking Union Medical College Hospital (PUMCH) from 2000 to 2005 were analyzed retrospectively using SPSS 11.0 software (Chicago, Illinois).Between 2000 and 2005, 635 patients with gestational trophoblastic neoplasms were treated at PUMCH, 11 with PSTT (1.73%). The mean age was 36 years. The antecedent pregnancy was molar in 5 cases (45.5%), full-term delivery in 4 cases (36.4%) and missed abortion in 2 cases (18.2%). The mean interval from the antecedent pregnancy to diagnosis was 16 months. The most common presentations were vaginal bleeding (72.7%) and amenorrhea (63.6%). All patients were pathologically diagnosed, in most cases with human placental lactogen immunohistochemical stain. Chemotherapy and hysterectomy were performed on all patients. Nine complete remissions and 1 partial remission were attained after therapy.Pathologic diagnosis of PSTT was the gold standard. Multidrug chemotherapy combined with hysterectomy was effective in metastasis cases. (J Re
- Published
- 2007
58. Overexpression of Eukaryotic Translation Initiation Factor 5A2 (EIF5A2) Correlates with Cell Aggressiveness and Poor Survival in Gastric Cancer
- Author
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Jian-Chun Yu, Weiming Kang, Zhi-Qiang Ma, Quan-Cai Cui, Wei-Xun Zhou, Yu-Qin Liu, Li Zhou, and Qing-Bin Meng
- Subjects
Male ,Lymphovascular invasion ,Science ,Cell ,Gene Expression ,Biology ,Histone Deacetylases ,Gene Knockout Techniques ,Cyclin D1 ,Peptide Initiation Factors ,Stomach Neoplasms ,Cell Line, Tumor ,medicine ,Humans ,RNA, Small Interfering ,Cyclin D3 ,Aged ,Cell Proliferation ,Neoplasm Staging ,Aged, 80 and over ,Multidisciplinary ,Cell growth ,RNA-Binding Proteins ,Cancer ,Transfection ,Middle Aged ,Prognosis ,medicine.disease ,Molecular biology ,Tumor Burden ,Repressor Proteins ,medicine.anatomical_structure ,Tumor progression ,Disease Progression ,Trans-Activators ,Medicine ,Female ,RNA Interference ,Research Article - Abstract
Eukaryotic translation initiation factor 5A2 (EIF5A2) plays an important role in tumor progression and prognosis evaluation. However, little information is available about its potential role in gastric cancer. This study aimed to investigate the function of EIF5A2 in tumor progression and its potential mechanisms. EIF5A2 expression was measured in human gastric cancer cell lines, the immortalized gastric mucosal epithelial cell line (GES-1) and human gastric cancer tissues and knocked down by RNA interference or upregulated by EIF5A2 plasmid transfection. Cell proliferation, migration and invasion were assessed in vitro. The downstream targets of EIF5A2 were examined by western blotting. EIF5A2 and its potential target metastasis-associated protein 1 (MTA1) expression were examined in 160 pairs of human gastric cancer and adjacent non-tumor specimens using immunohistochemistry (IHC) staining, and its correlation with clinicopathological features and survival was investigated. Knockdown of EIF5A2 or MTA1 caused an apparent suppression of HGC27 cell proliferation, migration and invasion. After knockdown of EIF5A2 in HGC27 cells, E-cadherin levels were upregulated and vimentin, cyclin D1, cyclin D3, C-MYC and MTA1 levels were downregulated. Upregulation of EIF5A2 in MKN45 cells resulted in the converse. IHC results showed a positive correlation between EIF5A2 and MTA1 expression in gastric cancers (P
- Published
- 2015
59. [Advances in primary thyroid lymphoma]
- Author
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Yuan, Li, Ding-rong, Zhong, and Quan-cai, Cui
- Subjects
Lymphoma ,Humans ,Thyroid Neoplasms - Abstract
Primary thyroid lymphomas (PTLs) are closely correlated with the autoimmune reaction of thyroid. However, the molecular mechanisms of PTLs are still unclear. It is really necessary to improve the ability to differentiate between benign and malignant PTLs along with the introduction of some new molecular biology methods. The diagnosis and prognosis of PTLs depend on their histological features, pathological classification, and clinical stages. Customized therapy of PTLs becomes possible with the further advances in lymphoma's pathological classification, clinical stages, and international prognosis index standard.
- Published
- 2006
60. [Genetic heterogeneity for familial recurrent hydatidiform mole]
- Author
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Jun, Zhao, Yang, Xiang, Shang-zhi, Huang, Xi-run, Wan, Quan-cai, Cui, M J, Seckl, and R A, Fisher
- Subjects
Family Health ,Male ,Genetic Heterogeneity ,Genotype ,Haplotypes ,Pregnancy ,Humans ,Female ,Genetic Predisposition to Disease ,Hydatidiform Mole ,Neoplasm Recurrence, Local ,Pedigree - Abstract
To determine the parental origin of the genome in the molar pregnancies of two familes with familial recurrent hydatidiform mole (FRHM) and to investigate whether the gene responsible for FRHM is likely to be located within the 19q13.4 region in these familes.The features of complete hydatidiform mole (CHM) were confirmed by pathological examination. DNA of CHM was prepared from sections of formalin-fixed paraffin-embedded blocks of molar tissue following laser capture microdissection. The polymerace chain reaction was used to amplify microsatellite polymorphisms in DNA from the patients, their husbands and the captured molar tissue. Parental contributions to the molar tissue were determined using ABI 310 GeneScan software. Genotyping and haplotype analysis of the candidate region on 19q13.4 was performed for members of both families using 25 microsatellite markers.One CHM from each family was identified as a biparental complete hydatidiform mole. All patients were heterozygous for most of the markers in the chromosome region of interest. In addition the two affected sisters in one of the families had different genotypes for the 19q13.4 region, suggesting that mutations in a different locus might be responsible for the disorder in this family.The location of the gene responsible for FRHM is unlikely to be located in the 19q13.4 chromosomal region in these two families suggesting that FRHM shows genetic heterogeneity.
- Published
- 2006
61. [Gene rearrangement studies in Hashimoto's thyroiditis and primary lymphoma of thyroid]
- Author
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Zhen, Huo, Yuan, Li, Ding-rong, Zhong, and Quan-cai, Cui
- Subjects
Gene Rearrangement ,Male ,Sex Characteristics ,Lymphoma ,Thyroiditis, Autoimmune ,Gender Identity ,Humans ,Female ,Hashimoto Disease ,Thyroid Neoplasms ,Middle Aged - Abstract
To study the immunoglobulin gene rearrangement patterns in Hashimoto's thyroiditis (HT) and primary thyroid lymphoma (PTL), and to analyze the relationship between the two diseases.Formalin-fixed and paraffin-embedded tissues of 11 cases of PTL and 38 cases of HT as well as their clinical data, were retrieved. The latter group was further subcategorized into classic HT and suspicious PTL. Gene rearrangement studies for immunoglobulin heavy chains and light chains were carried out by polymerase chain reaction (PCR) using VH, FR3A and FR3kappa primers.There was an increasing trend in immunoglobulin gene rearrangement rate for classic HT (10.7%), suspicious PTL (40.0%) and PTL (72.7%) groups. In general, a female predilection was observed. This sex predilection however was less obvious in the PTL group. There was no relationship between serum antibody (both thyroglobulin and thyroid peroxidase) titers and gene rearrangement patterns.HT and PTL show morphologic overlaps and may not be clearly distinguished on the basis of light microscopy alone. PCR-based immunoglobulin gene rearrangement study may be helpful in the detection of cases with early lymphomatous transformation of HT.
- Published
- 2006
62. [Visual and histologic analysis of laparoscopic diagnosis of endometriosis]
- Author
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Jin-hua, Leng, Jing-he, Lang, Xue-ying, Zhao, Hua-jun, Li, Li-na, Guo, and Quan-cai, Cui
- Subjects
Adult ,Uterine Diseases ,Evaluation Studies as Topic ,Biopsy ,Ovary ,Endometriosis ,Humans ,Female ,Laparoscopy ,Prospective Studies ,Sensitivity and Specificity ,Pelvis - Abstract
To determine the characteristics of anatomical distribution of pelvic endometriosis and the correlation between visual and histologic findings of endometriosis at laparoscopy.A prospective study of 62 patients undergoing laparoscopy for the pelvic pain, infertility and/or pelvic masses was carried out. All lesions with the diagnosis of endometriosis laparoscopically were excised and examined pathologically. Normal-appearing peritoneal biopsies were obtained randomly. All lesions were identified by anatomical site and color of the foci. The positive predictive value (PPV), sensitivity, negative predictive value (NPV), and specificity were determined for visually identified endometriosis versus the histologic findings.Totally, 219 peritoneal endometriotic lesions, 54 normal peritoneal biopsies, and 71 ovarian endometriotic cysts were obtained. Peritoneal lesions tended to locate in posterior part of the pelvis (80.8%, 177/219) and in left (58.0%, 127/219) with most in black (39.2%). The PPV was 67.6%; sensitivity, 93.7%; NPV, 81.4%; and specificity, 38.3% for visual versus histologic diagnosis of peritoneal endometriosis. Lesions in black or from sacral ligaments were confirmed histologically in 94.2% and 84.7% respectively, and 80.3% (57/71) of ovarian endometriotic cysts diagnosed by laparoscopy were confirmed histologically with 43.6% in the left, 27.3% in the right; and 29.1% (16/55) in both sides of the ovary. In addition, 18.5% (10/54) of normal-appearing peritoneal biopsy were identified as endometriosis by pathological examination. Laparoscopy was confirmed to be in 100% diagnostic accordance with pathology for patients with endometriosis.Our study showed asymmetrical distribution of pelvic endometriosis. Peritoneal lesions in black or from sacral ligament are more likely to be histologically confirmed, and microscopic lesions are not a rare phenomenon of endometriosis.
- Published
- 2006
63. [Clinicopathologic study of 10 cases of osteomalacia or rickets-associated mesenchymal tumors]
- Author
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Ding-Rong, Zhong, Tong-Hua, Liu, Di, Yang, Rui-E, Feng, Quan-Cai, Cui, Yu-Feng, Luo, and Yong, Jia
- Subjects
Adult ,Male ,Femoral Neoplasms ,Antigens, CD34 ,Bone Neoplasms ,Soft Tissue Neoplasms ,Middle Aged ,Actins ,Osteomalacia ,Humans ,Mesenchymoma ,Vimentin ,Female ,Aged ,Rickets - Abstract
To study the clinicopathologic features of osteomalacia or rickets-associated mesenchymal tumors.The clinical and pathologic findings of 10 cases of osteomalacia or rickets-associated mesenchymal tumors were evaluated. Hematoxylin and eosin stain, immunohistochemistry and histochemistry were performed on the archival paraffin sections.Amongst the 10 patients studied, 6 were males and 4 were females. Their age at the time of operation ranged from 28 to 69 years ( mean = 45.6 years). A history of long-standing bone pain, arthralgia, limitation in movement, hypophosphatemia and hyperphosphaturia was present in all cases. The duration of symptoms ranged from 2 to 27 years (mean = 9.6 years). The tumor size ranged from 1 to 7 cm (mean size = 3.52 cm). Microscopically, the tumors were composed of various mesenchymal cells, including spindled fibroblast-like cells, adipocytes, chondroid cells and mucinous cells. The background was rich in blood vessels. In 8 of the 10 cases, there was also dystrophic calcification in an unusual flocculent or "grungy" pattern. Peripheral woven bone shell formation was noted in 2 cases and non-urate crystal deposition in 2 cases. Mitotic figures were rare in 9 cases. In 1 of the 10 cases however, mitotic figures and bizarre cells were commonly encountered. On immunohistochemical study, the tumor cells were all positive for vimentin. There was focal positivity for smooth muscle actin and CD34 in 5 and 3 cases respectively. The staining for desmin, S-100 and AE1/AE3 was negative. Ki-67 proliferation index was less than 4% in 8 cases and 30% in 1 case. Alcian blue-positive mucinous matrix and mucinous degeneration around vessels were noted in 8 cases.Most of the osteomalacia or rickets-associated tumors are either benign or low-grade malignant mesenchymal tumors. They can be mistaken as other neoplasms due to the morphologic heterogeneity present. Thorough understanding of the associated clinical features and laboratory investigation results is helpful in arriving at the correct diagnosis.
- Published
- 2006
64. Endophthalmitis induced by mycobacterium tuberculosis being misdiagnosed as non-infectious uveitis
- Author
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Han-yi, Min and Quan-cai, Cui
- Subjects
Adult ,Uveitis ,Endophthalmitis ,Humans ,Female ,Mycobacterium tuberculosis ,Diagnostic Errors ,Tuberculosis, Pulmonary - Published
- 2006
65. [The clinical and pathological characteristics and prognosis of pseudomyxoma peritonei]
- Author
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Zhi-qiang, Song, Wen-ze, Wang, Xing-hua, Lu, Mei-yun, Ke, Xiao-hong, Sun, and Quan-cai, Cui
- Subjects
Adult ,Male ,Adolescent ,Humans ,Female ,Middle Aged ,Prognosis ,Pseudomyxoma Peritonei ,Survival Analysis ,Peritoneal Neoplasms ,Aged ,Follow-Up Studies ,Retrospective Studies - Abstract
To investigate the clinical and pathological characteristics, treatment measures and prognosis of pseudomyxoma peritonei (PMP).The clinical records and follow-up data of the patients with PMP were retrospectively analyzed. Survival analysis (Kaplan-Meier method and Cox regression) was used to investigate the prognosis.Thirty-three patients (twenty-two women, eleven men) have a median interval between onset and definite diagnosis of 12 months with an average age of 50. The main clinical presentation includes bloating, abdominal mass and abdominal distention. A delay in diagnosis is common and many patients are labeled as other diseases (84.8%). Positive results were often found in the assistant examinations such as erythrocyte sedimentation rate, gastrointestinal tumor markers (carcinoembryonic antigen, carcinomatous antigen 19-9 and so on), imaging (B ultrasound and computer tomography) and abdominal paracentesis which was usually unsuccessful. The most frequently practiced approach is debulking surgery with the main assistant means of chemotherapy which was lack of the uniform rationale. Benign type of pathological samples accounts for 66.7% of all, while mid-type 21.2% and malignant 12.1%. The general median survival time is 70 months with a follow-up rate of 79%. The pathological type and chemotherapy are main factors which influence the cumulative survival rate (P0.05). Up to the end of 2004, sixteen patients died of various complications, multiple organ failure and severe infection, while ten patients survival and seven patients lost.PMP is a rare condition of borderline malignancy with its unique clinical and pathological characteristics. Gastrointestinal tumor markers, imaging and abdominal paracentesis are very important to the diagnosis. The existing treatment measures need to be improved further. The pathological type and chemotherapy are main factors which influence the prognosis.
- Published
- 2006
66. [Clinicopathologic features and surgical treatment of nonfunctioning islet cell tumors (78 case report)]
- Author
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Tai-ping, Zhang, Yu-pei, Zhao, Yu, Zhu, Li-xing, Cai, Quan-cai, Cui, and Jie, Chen
- Subjects
Adult ,Male ,Adolescent ,Middle Aged ,Adenoma, Islet Cell ,Prognosis ,Pancreatic Neoplasms ,Young Adult ,Humans ,Female ,Child ,Aged ,Follow-Up Studies ,Retrospective Studies - Abstract
To investigate surgical treatment results and clinical pathological specifics in the treatment of non-functional islet cell tumor.To perform retrospective analysis of 78 cases of non-functional islet cell tumor treated at Peking Union Medical College Hospital from July 1968 through January 2005, and summarize clinical symptoms and signs, primary diagnosis before surgery, surgical treatment, pathologic specifics and immuno-histological analysis.Of 78 cases, 27 cases (34.6%) was found in physical examinations, the most common symptoms was upper abdominal pain and upper abdominal discomfort, 30.7% (24 cases) and 26.9% (21 cases) respectively; the most common sign being abdominal masses 35.9% (28 cases). B-us and CT are most commonly used forms of preoperative examination, with positive rates being 98.6% (71/72), 100% (53/53) respectively. Operative mortality was 1.28%, Radical surgery of malignant cases with 5 year survival rate 75% (9/12). Immuno-histological examinations showed that tumors with multi peptide linked hormones 78.6% (55/70), a larger number of positive examinations of neuron specific enolase and chromaffin, 91.4% (32/35) and 79.4% (27/34) respectively. Insulin, glucagon, pancreatic polypeptide, somatostatin, vasoactive intestinal peptide and gastrin positive rates are 64.6% (42/65), 47.5% (22/53), 45.8% (22/48), 37.5% (21/56), 23.9% (11/46) and 22.6% (7/31).Non-functional islet cell tumor lack specificity, Bus and CT are primary examination methods, immuno-histological analysis indicates various different hormones, but lacks the presence of related clinical symptoms. Surgery is an effective form of treatment in the treatment of non-functional islet cell tumor, even with distal metastasis, immediate surgical removal and treatment, extend prognosis.
- Published
- 2005
67. Analysis of the chromosomal region 19q13.4 in two Chinese families with recurrent hydatidiform mole
- Author
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Michael J. Seckl, Neil J. Sebire, Jun Zhao, Jill Moss, Quan-cai Cui, Yang Xiang, and Rosemary A. Fisher
- Subjects
Male ,China ,Genotype ,Biology ,Genetic Heterogeneity ,Pregnancy ,Polymorphic Microsatellite Marker ,Humans ,Genotyping ,Genetics ,Genetic heterogeneity ,Rehabilitation ,Haplotype ,Obstetrics and Gynecology ,Hydatidiform Mole ,NLRP7 ,Pedigree ,Reproductive Medicine ,Haplotypes ,Chromosomal region ,Cytogenetic Analysis ,Uterine Neoplasms ,Microsatellite ,Female ,Chorionic Villi ,Neoplasm Recurrence, Local ,Chromosomes, Human, Pair 19 ,Microsatellite Repeats - Abstract
BACKGROUND: Familial recurrent hydatidiform mole is an extremely rare autosomal recessive condition in which affected individuals have a predisposition to molar pregnancies that are diploid but biparental, rather than androgenetic, in origin. A gene for this condition has been previously mapped to a 1.1 Mb region of chromosome 19q13.4. However, investigation of further families is needed to refine the location of the specific gene(s) involved. METHODS: We have recently identified two novel Chinese families in which four affected women had recurrent pregnancy loss including 14 complete hydatidiform moles (CHM). Fluorescent microsatellite genotyping was used to determine the origin of CHM in both families. Using a panel of polymorphic microsatellite markers, genotyping and haplotype analysis of the 19q13.4 chromosomal region was performed in both families. RESULTS: Genotyping of CHM from affected individuals confirmed their biparental origin and diagnosis of familial recurrent hydatidiform mole in both families. However, no significant homozygosity for the 19q13.4 candidate region was found in affected members of either family. CONCLUSION: Genotyping and haplotype analysis has shown that a mutation in 19q13.4 is unlikely to be responsible for recurrent CHM in the two Chinese families investigated and provides further evidence to support the hypothesis that, although extremely rare, this condition shows genetic heterogeneity.
- Published
- 2005
68. Clinical diagnosis and treatment of nonfunctioning pheochromocytomas in 14 patients
- Author
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Dong-liang, Pan, Han-zhong, Li, Zheng-pei, Zeng, Fang, Li, and Quan-cai, Cui
- Subjects
Adult ,Male ,3-Iodobenzylguanidine ,Adrenal Gland Neoplasms ,Humans ,Female ,Pheochromocytoma ,Middle Aged ,Radiopharmaceuticals ,Radionuclide Imaging ,Tomography, X-Ray Computed ,Follow-Up Studies ,Retrospective Studies - Abstract
To analyze the clinical characteristics of nonfunctioning pheochromocytoma, and to evaluate the efficacy of 131I-metaiodobenzylguanidine (MIBG) scan in the diagnosis and perioperative treatment of nonfunctioning pheochromocytoma.The clinical data of 14 patients with nonfunctioning pheochromocytoma were analyzed retrospectively. Plasma free corticoid, renin, aldosterone, and urine catecholamines levels were estimated. B-mode ultrasonography, computed tomography scan, thoracic X-ray and 131I-MIBG were used.All patients with nonfunctioning pheochromocytoma had no hypertension and the tumors were found incidentally. The 24 hours urine catecholamines levels in 80% (8/10) patients were normal. The positive rate of 131-MIBG was 80% (8/10) and the specificity was 100%. All patients underwent surgical operation of tumor resection. No preoperative volume expansion was given to all patients. All tumors were resected completely, and no death accident happened. There was no recurrence and metastasis after operation by long-term follow-up.131I-MIBG scan is the first choice technique for the diagnosis of nonfunctioning pheochromocytoma. Blood volume expansion is unnecessary before resection of pheochromocytoma.
- Published
- 2005
69. Twenty-six patients with nonfunctional pheochromocytomas
- Author
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Dong-liang, Pan, Han-zhong, Li, Zheng-pei, Zeng, Fang, Li, and Quan-cai, Cui
- Subjects
Adult ,Male ,3-Iodobenzylguanidine ,Adrenal Gland Neoplasms ,Humans ,Female ,Pheochromocytoma ,Middle Aged ,Radionuclide Imaging - Published
- 2005
70. [Expression of survivin gene in pancreatic carcinoma]
- Author
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Zhi-Yong, Liang, Tong-Hua, Liu, Yu-Feng, Luo, Jian, Guan, Shuan-Zeng, Wei, Jin-Ling, Cao, Jie, Gao, and Quan-Cai, Cui
- Subjects
Male ,Survivin ,Adenocarcinoma, Mucinous ,Inhibitor of Apoptosis Proteins ,Neoplasm Proteins ,Gene Expression Regulation, Neoplastic ,Pancreatic Neoplasms ,Cell Line, Tumor ,Biomarkers, Tumor ,Humans ,Female ,RNA, Messenger ,Microtubule-Associated Proteins ,Carcinoma, Pancreatic Ductal ,Neoplasm Staging - Abstract
To determine the expression status of survivin gene in pancreatic carcinoma.Expression of survivin gene was evaluated by immunohistochemistry, Western Blot and RT-PCR in 59 cases of pancreatic carcinoma along with their corresponding adjacent benign tissues, 11 cases of chronic pancreatitis, and 7 pancreatic carcinoma cell lines.The positive expression rate of survivin in pancreatic carcinoma was 72.8% (43/59). There was no relationship between the expression of survivin and tumor stage and differentiation. No expression of survivin was detected in benign tissue adjacent to the tumors as well as in samples of chronic pancreatitis. All 7 pancreatic carcinoma cell lines showed a positive expression of survivin mRNA and protein.The expression of survivin appears to be tumor specific to some extent in our pancreatic carcinoma samples. Survivin may be an ideal target for therapy against pancreatic carcinoma.
- Published
- 2005
71. Intraspinal clear cell meningioma: a case report
- Author
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Yong, Jia, Ding-rong, Zhong, and Quan-cai, Cui
- Subjects
Adult ,Cauda Equina ,Meningeal Neoplasms ,Humans ,Female ,Spinal Cord Neoplasms ,Meningioma ,Magnetic Resonance Imaging - Published
- 2005
72. [The advances of molecular pathology of follicular thyroid carcinoma]
- Author
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Yang, Zhan and Quan-Cai, Cui
- Subjects
Diagnosis, Differential ,Adenocarcinoma, Follicular ,Biomarkers, Tumor ,ras Proteins ,Humans ,Thyroid Neoplasms ,Matrix Metalloproteinase 1 ,Telomerase - Published
- 2004
73. [Recent advances in gene change of pancreatic cancer]
- Author
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Yu-yue, Wang and Quan-cai, Cui
- Subjects
Fibroblast Growth Factors ,Pancreatic Neoplasms ,Genes, ras ,Epidermal Growth Factor ,Genes, p16 ,Genes, myc ,Humans ,Genes, Tumor Suppressor ,Oncogenes ,Genes, p53 ,Growth Substances - Abstract
A large number of data derived from molecular analyses support the hypothesis that human cancer is a genetic disease and a distinct subset of genes have been found to be genetically changed in most tumors. Molecular alterations in pancreatic cancer include: (1) oncogenes such as K-ras, c-myc, c-fos, and c-erbB-2; (2) tumor suppressor genes such as p53, p16, DPC4/SMAD4, and DCC; and (3) growth factors such as EGF, FGF, HGF, PDGF, VEGF, TGF-beta. Genetic alterations of K-ras and p53 are common in human pancreatic cancer, but the occurrence of pancreatic cancer is a multi-step phenomenon in which the accumulation of genetic changes is extremely important.
- Published
- 2004
74. Uterine adenomyoma with lymphoid infiltration simulating lymphoma
- Author
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Ruie Feng, Yufeng Luo, Shuying Zhang, Shuanzeng Wei, and Quan-Cai Cui
- Subjects
Adult ,Pathology ,medicine.medical_specialty ,Uterine leiomyoma ,Lymphoma ,business.industry ,Obstetrics and Gynecology ,medicine.disease ,Malignant lymphoma ,Diagnosis, Differential ,Oncology ,Stroma ,Lymphatic Metastasis ,Uterine Neoplasms ,medicine ,Humans ,Cyst ,Female ,business ,Infiltration (medical) ,Adenomyoma ,Rare disease - Abstract
Background. Uterine leiomyoma with lymphoid infiltration is a rare disease that simulates malignant lymphoma with only nine cases reported to date. We describe the first case of uterine adenomyoma with lymphoid infiltration simulating lymphoma. Case. The specimen resected from a 30-year-old Chinese woman was a well-defined firm nodule measuring 5 × 5.5 × 5.5 cm. The cut surface was similar to that of adenomyoma, which contained dark brown spots and a cyst. Microscopically, the tumor comprised smooth muscle cells intermixed with many lymphocytes. Many lymphoid follicles were present, just locating beside one side of thin-walled blood vessels and protruding into the vessels. Endometrial-type glands and stroma were visible in the tumor. Conclusion. We describe the first case of uterine adenomyoma with lymphoid infiltration simulating lymphoma.
- Published
- 2004
75. [Retrospective analysis of 27 cases of disseminated tuberculosis]
- Author
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Xiao-tong, Zhang, Long-yun, Li, Li, Zhang, Yuan-jue, Zhu, and Quan-cai, Cui
- Subjects
Adult ,Aged, 80 and over ,Male ,Adolescent ,Middle Aged ,Diagnosis, Differential ,Liver ,Humans ,Tuberculosis ,Female ,Autopsy ,Lymph Nodes ,Diagnostic Errors ,Lung ,Spleen ,Aged ,Retrospective Studies - Abstract
To analyze the clinical characteristics and treatment of hematogenous disseminated tuberculosis.Twenty-seven cases of hematogenous disseminated tuberculosis diagnosed by autopsy from 1961 to 2000 were retrospectively analyzed.Among these patients the disease was acute in 22 cases (including 3 non-reactive) and chronic in 5. Twenty cases (74%) were misdiagnosed before death, most of which were due to concomitant with or misdiagnosed as connective tissue disease, hematological disease or tumor. 63% of the cases had long-term use of corticosteroids or repeated chemotherapy. Most cases showed atypical clinical manifestations and chest X-ray lesions. The positive rate of tuberculin skin test and sputum for smear were low. Autopsy showed that all cases had active lung tuberculosis and the major extra-pulmonary organs involved were liver, spleen and lymph nodes.Unaware of the risk for tuberculosis among immune compromised patients was the main reason for misdiagnosis and corticosteroid abuse was common. Physician should be alert to the occurrence of hematogenous disseminated tuberculosis, especially in immune compromised patients with long-term fever and systemic injury. Direct smear and cultural examination as well as newer diagnostic approaches with sputum and tissue may be useful for a rapid and correct diagnosis.
- Published
- 2004
76. [Alterations of DPC4/SMAD4/MADH4 gene detected in paraffin-embedded tissues of human pancreatic carcinomas]
- Author
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Li-jun, Gu, Jie, Chen, Tong-hua, Liu, Quan-cai, Cui, Zhao-hui, Lu, Li, Li, and Jie, Gao
- Subjects
Adult ,Male ,Paraffin Embedding ,Middle Aged ,DNA-Binding Proteins ,Pancreatic Neoplasms ,Mutation ,Proto-Oncogenes ,Trans-Activators ,Humans ,Female ,RNA, Messenger ,Aged ,Smad4 Protein - Abstract
To demonstrate the alterations of DPC4/SMAD4/MADH4 gene in paraffin-embedded tissues of pancreatic carcinomas.Forty-six cases of resected specimens containing carcinomatous tissue and normal pancreatic tissue were analysed for possible DPC4 gene mutations by polymerase chain reaction (PCR)and single-strand conformation polymorphism (SSCP). The DNA sequencing technique was applied to determine the patterns of gene mutation in the PCR-SSCP positive cases. Fifty-six cases of pancreatic carcinoma along with the specimens corresponding normal pancreatic tissues were studied by in situ hybridization (ISH) and immunohistochemistry (IHC) techniques for gene expression in mRNA and protein level.The homozygous deletion rate of exon 1, 2, 3, 4, 8, 11 of DPC4 gene in pancreatic carcinoma was 28.26%, while the mutation rate of DPC4 gene was 21.74%. In these tumors, there were 3 cases of nonsense mutation, 5 cases of missense mutation, 1 case of deletion and missense mutation, 1 case of insertion mutation. Positive rates of SMAD4 in carcinomatous tissues detected by the ISH and IHC were 53.57% and 58.93% respectively, whereas they were 91.07% and 89.29% in the matched normal tissue respectively. There were significant difference between cancer and normal tissue (P0.05). Thrity-two cases were positive of DPC4/SMAD4 with all methods mentioned above, the coincident rate was 87.50% (28/32). The coincidence between gene detection and ISH of SMAD4 was 87.50%, and it was 96.88% between gene detection and IHC of SMAD4. Of all 56 cases, the coincidence of the positive rates of SMAD4 detected by ISH and IHC was 91.07%. No significant difference among the positive rates of DPC4/SMAD4 as detected by the three different techniques (P0.05).The main mechanisms of inactivation of DPC4 gene in pancreatic carcinoma are homozygous deletion and mutation. The product of DPC4 expression is significantly decreased in cancer group compared with the normal tissues. As a tumor suppressor gene, DPC4 alteration is an important molecular event in pancreatic carcinoma, and probably plays a crucial role in cancer development and progression.
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- 2003
77. Tumor heterogeneity in the recurrence of epithelial ovarian cancer demonstrated by polycomb group proteins
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Quan-Cai Cui, Jiaxin Yang, Huimin Bai, Dongyan Cao, Keng Shen, Ting Gui, Zhaoji Zhong, Jianfang Zeng, and Jie Chen
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Tissue microarray ,PcG protein ,business.industry ,EZH2 ,macromolecular substances ,Bioinformatics ,OncoTargets and Therapy ,medicine.anatomical_structure ,Oncology ,BMI1 ,Ovarian carcinoma ,Gene duplication ,microRNA ,Cancer research ,Medicine ,Pharmacology (medical) ,business ,Lymph node ,Survival analysis ,Original Research ,miRNA - Abstract
Ting Gui,1,* Huimin Bai,1,* Jianfang Zeng,1 Zhaoji Zhong,1 Dongyan Cao,1 Quancai Cui,2 Jie Chen,2 Jiaxin Yang,1 Keng Shen11Department of Obstetrics and Gynecology, 2Department of Pathology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China *These authors contributed equally to this work Purpose: To investigate tumor heterogeneity in the recurrence of epithelial ovarian cancer demonstrated by polycomb group (PcG) proteins. Methods: Tissue microarrays containing matched primary and recurrent ovarian tumors from the same patients were constructed for detection of PcG protein expression. Survival analyses of clinicopathological parameters and expression of PcG proteins were performed on progression-free survival (PFS) and overall survival (OS) of patients. Genetic and epigenetic heterogeneity was explored in aspects of gene copy number and microRNA (miRNA) profiling. Results: PcG proteins were heterogeneously expressed in primary versus recurrent tumors (P
- Published
- 2014
78. Novel HRPT2/CDC73 Gene Mutations and Loss of Expression of Parafibromin in Chinese Patients with Clinically Sporadic Parathyroid Carcinomas
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Ou Wang, Weibo Xia, Xunwu Meng, Quan-cai Cui, Chunyan Wang, Yan Jiang, Heng Guan, Xiaoping Xing, Mei-Mei Li, and Min Nie
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Male ,Pathology ,lcsh:Medicine ,Gene Expression ,Gene mutation ,medicine.disease_cause ,Stage (cooking) ,lcsh:Science ,Endocrine Tumors ,Regulation of gene expression ,Mutation ,Multidisciplinary ,Parathyroid neoplasm ,Middle Aged ,Gene Expression Regulation, Neoplastic ,Parathyroid Neoplasms ,Oncology ,Parathyroid carcinoma ,Medicine ,Female ,Research Article ,Adult ,China ,medicine.medical_specialty ,Tumor suppressor gene ,Molecular Sequence Data ,Parafibromin ,Biology ,Young Adult ,Asian People ,Genetics ,Cancer Genetics ,Cancer Detection and Diagnosis ,medicine ,Humans ,Genetic Testing ,Genetic Association Studies ,Aged ,Evolutionary Biology ,Base Sequence ,Tumor Suppressor Proteins ,lcsh:R ,Cancers and Neoplasms ,Human Genetics ,medicine.disease ,Genetics of Disease ,Parathyroid Carcinoma ,lcsh:Q ,Population Genetics - Abstract
OBJECTIVE: It is widely recognized that the diagnosis of parathyroid carcinoma (PC) is often difficult because of the overlap of characteristics between malignant and benign parathyroid tumors, especially at an early stage. Based on the identification of tumor suppressor gene HRPT2/CDC73 and its association with hereditary and sporadic PC, screening of gene mutations and detection of parafibromin immunoreactivity have been suggested as diagnostic instruments of PC in Whites. There is little information about HRPT2/CDC73 mutations and its corresponding protein expression in patients with sporadic PC in Chinese population, and the long-term follow-up data is scarce. METHODS: Paraffin-embedded tissues were obtained from 13 patients with PC, 13 patients with parathyroid adenoma (PA) and 7 patients with parathyroid hyperplasia(PH), and 6 normal parathyroid (NP) tissues as controls. Peripheral blood from 11 patients with PC was collected. PCR products using Genomic DNA extracted from tumor tissues or blood as template was sequenced for HRPT2/CDC73 gene. Expression of parafibromin in tumor tissues was evaluated by immunohistochemical analysis. RESULTS: Six mutations in 6 of 13 patients with PC were identified, with three being novel. Four of them were germ-line mutations. Patients with mutations were susceptible to recurrence of the PC. Complete (8/13, 61.5%) or partial (5/13, 38.5%) loss of parafibromin expression was observed in PC tissues. All of tissue samples from normal parathyroid or benign parathyroid tumors displayed positive immunostaining of parafibromin except one adenoma. CONCLUSIONS: The present study supplies information on the mutations and protein expression of HRPT2/CDC73 gene and phenotypes of parathyroid carcinoma in Chinese population. And the expanded mutation database of this gene may benefit patients in the diagnosis and treatment of this disease.
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- 2012
79. Significance of the Detection of Ki-ras Codon 12 Mutation in the Diagnosis and Differential Diagnosis of Pancreatic Carcinoma.
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Tong-hua Liu, Zhi-yong Wang, and Quan-cai Cui
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PANCREATIC cancer ,NEEDLE biopsy ,POLYMERASE chain reaction ,CHROMOSOME polymorphism ,PANCREATIC diseases - Abstract
Ki-ras codon 12 mutation was detected in: (1) surgically resected formalin-fixed and paraffin-embedded specimens from 109 carcinoma cases, including 46 pancreatic, 35 ampullary, 24 extrahepatic bile duct, and 4 gallbladder carcinomas, showing prevalences of 95.7%, 5.7%, 8.3%, and 0%, respectively, by polymerase chain reaction-restriction fragment length polymorphism technique, and 89.1%, 2.9%, 8.3%, and 0%, respectively, by polymerase chain reaction-dot blot method; and (2) 35 consecutive fine-needle aspiration biopsy specimens by polymerase chain reaction-restriction fragment length polymorphism giving a positive rate of 94.7% (18/19) for pancreatic carcinoma and 0% (0/16) in the specimens from nonpancreatic carcinoma tissues or tumors. Our results demonstrate that the strikingly high prevalence of Ki-ras codon 12 mutation of pancreatic carcinoma could be used as an effective means in the diagnosis and differential diagnosis of pancreatic carcinoma. [ABSTRACT FROM AUTHOR]
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- 1995
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80. Watery Diarrhea Syndrome Caused by Multihormonal Malignant Pancreatic Islet Cell Tumor Secreting Somatostatin, Vasoactive Intestinal Peptide, Serotonin, and Prostaglandin E—A Clinicopathological, Biochemical, Immunohistochemical, and Ultrastructural Study
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Yuan Fang Chen, Tong-Hua Liu, Shou-Po Chen, Guo-Zong Pan, Xing-Hua Lu, Guo-Jun Lu, Shou-Xiang Zhong, Li-Xing Cai, Quan-Cai Cui, Qing-Yun Ran, Xue Hou, Hsien Chiu Tseng, and Min Chang Chen
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Adult ,Male ,Serotonin ,endocrine system ,medicine.medical_specialty ,Pancreatic disease ,Adenoma ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Vasoactive intestinal peptide ,Prostaglandin ,Biology ,Peptide hormone ,chemistry.chemical_compound ,Endocrinology ,Internal medicine ,Internal Medicine ,medicine ,Humans ,Hormone metabolism ,Hepatology ,Histocytochemistry ,Prostaglandins E ,Adenoma, Islet Cell ,medicine.disease ,Hormones ,Pancreatic Neoplasms ,Somatostatin ,chemistry ,Vipoma ,hormones, hormone substitutes, and hormone antagonists ,Vasoactive Intestinal Peptide ,Prostaglandin E - Abstract
The pathophysiological, biochemical, histological, ultrastructural, and immunohistochemical characters of a case of malignant pancreatic islet cell tumor with watery diarrhea syndrome were carefully investigated. Four hormones or mediators--somatostatin (SST), vasoactive intestinal peptide (VIP), serotonin, and prostaglandin E--were markedly elevated in the circulation. The diagnosis was further confirmed by exploratory laparotomy and autopsy. The contents of SST and VIP in tumor tissues were very high. Gel chromatography of tumor extract revealed single peaks for both SST and VIP. Immunohistochemical studies of tumor tissues showed numerous immunoreactive cells to anti-SST, moderate amount of VIP-positive cells, and a few hCG-, insulin-, and glucagon-positive cells. In conclusion, this is an unusual case of Verner-Morrison syndrome in which three kinds of bioactive hormones or mediators were simultaneously secreted; peptides, amine, and prostaglandin.
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- 1986
81. Insulinoma. An immunocytochemical and morphologic analysis of 95 cases
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Jie Chen, Hsien-Chiu Tseng, Quan-Cai Cui, Liu T, Yu Zhu, and Shou-Xian Zhong
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endocrine system ,Cancer Research ,medicine.medical_specialty ,Pathology ,Vasoactive intestinal peptide ,Biology ,medicine.disease ,Secretin ,Motilin ,Endocrinology ,Somatostatin ,Gastric inhibitory polypeptide ,Oncology ,Internal medicine ,medicine ,Pancreatic polypeptide ,Insulinoma ,hormones, hormone substitutes, and hormone antagonists ,Gastrin - Abstract
One hundred twenty-seven insulinomas from 95 cases (1 malignant and 94 benign) were studied pathologically. Thirty-six tumors (35 cases) were examined by electron microscopy. Typical beta-cell secretory granules of crystalloid-form cores and/or atypical secretory granules were discerned in all tumors examined. A new type of secretory granule, with high electron-dense crystalloid-form cores and moderate electron-dense granular substance filling the space between the core and the limiting membrane, were observed in two cases. Among 68 insulinomas (67 cases) subjected to immunocytochemical investigations with ten peptide hormones (insulin, glucagon, somatostatin, pancreatic polypeptide (PP), gastrin, motilin, secretin, vasoactive intestinal polypeptide (VIP), gastric inhibitory polypeptide (GIP), and neurotensin), 42 were found to be multihormonal, varying from two to four peptides secreted. The hormones contained were insulin, glucagon, PP, somatostatin, and gastrin in different combinations. One patient had hyperinsulinemia and hypergastrinemia concurrently, and two islet tumors were excised at an interval of 10 months. Both electron microscopy and immunocytochemistry confirmed the presence of beta- and alpha-cells in the first tumor, whereas the second tumor revealed only G-cells by electron microscopy, and G- and beta-cells on immunocytochemical staining. The morphologic and immunocytochemical characteristics of the insulinomas in this series are discussed.
- Published
- 1985
82. ENDOPHTHALMITIS INDUCED BY MYCOBACTERIUM TUBERCULOSIS BEING MISDIAGNOSED AS NON-INFECTIOUS UVEITIS.
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Han-Yi Min and Quan-Cai Cui
- Subjects
- *
VISUAL acuity , *VISION , *ANTERIOR chamber (Eye) , *ANTERIOR eye segment , *FUNDUS oculi - Abstract
The article presents the case of a 19-year-old female suffering from gradually decreasing visual acuity of left eye for 3 months, diagnosed at Posner-Schlossman syndrome due to the mild inflammation of anterior chamber and increase of intra-ocular pressure (IOP). Physical examination showed that for the right eye, the visual acuity was 0.3 IOP was 18 mmHg, and the anterior segment and the fundus was normal.
- Published
- 2005
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