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Purpose The purpose of this study was to investigate clinical efficacy of posterior tibial artery perforator technique combined with iliac crest autograft in treatment of medial soft tissue and medial malleolus loss. Methods This study involved 11 cases of medial soft tissue and medial malleolus loss from October 2011 to March 2016. Patients were treated with posterior tibial artery perforator technique combined with iliac crest autograft, and given routine treatment, such as rehydration, anti-inflammation, anticoagulation and vasodilation. Ankle function of patients was evaluated according to the American Orthopedic foot and ankle Association (AOFAS) ankle-hind foot scoring system. Results All flaps survived without bone exposure, and the appearance of skin flaps was satisfactory. There was one case of arterial crisis, one case of venous crisis, one case of skin edge necrosis and one case of incision infection. Wounds of the above patients were healed. Skin flap was soft and elastic without secondary contracture. The two-point discrimination of skin flap was 5–11 mm. The ankle range of motion was 10–60°. X-Ray showed that grafts healed within 8.6 months. According to AOFAS evaluation, four cases were excellent, four cases were good, and three cases were poor. The excellent and good rate was 72.8%. Conclusions In this study, posterior tibial artery perforator technique combined with iliac crest autograft was used to treat medial soft tissue and medial malleolus loss. The findings demonstrated that this treatment was reliable and efficacious.

Plokiophiloides bannaensis sp. nov., is described from Xishuangbanna, Yunnan Province, representing the first record of the family Plokiophilidae from China. The new species also represents the first record of the genus Plokiophiloides in the Oriental Region, a second zoogeographical region besides the Afrotropical Region. Photographs of the live individuals inhabiting a spider web within natural habitats, male and female habitus, wings of adult, male genitalic structures, female abdomen structures and scanning electron micrographs of forewing, head, thorax and legs are provided. A key to all known species of Plokiophiloides is presented, with a distribution map.

BackgroundThis research aimed to evaluate the impacts of transfusing packed red blood cells (pRBCs), fresh frozen plasma (FFP), or platelet concentrate (PC) on postoperative mechanical ventilation time (MVT) in patients with acute Stanford type A aortic dissection (ATAAD) undergoing after total arch replacement (TAR).MethodsThe clinical data of 384 patients with ATAAD after TAR were retrospectively collected from December 2015 to October 2017 to verify whether pRBCs, FFP, or PC transfusion volumes were associated with postoperative MVT. The logistic regression was used to assess whether blood products were risk factors for prolonged mechanical ventilation (PMV) in all three endpoints (PMV ≥24 h, ≥48 h, and ≥72 h).ResultsThe mean age of 384 patients was 47.6 ± 10.689 years, and 301 (78.39%) patients were men. Median MVT was 29.5 (4–574) h (h), and 213 (55.47%), 136 (35.42%), and 96 (25.00%) patients had PMV ≥24 h, ≥48 h, and ≥72 h, respectively. A total of 36 (9.38%) patients did not have any blood product transfusion, the number of patients with transfusion of pRBCs, FFP, and PC were 334 (86.98%), 286 (74.48%), and 189 (49.22%), respectively. According to the multivariate logistic regression of three PMV time-endpoints, age was a risk factor [PMV ≥ 24 h odds ratio (ORPMV≥24) = 1.045, p = 0.005; ORPMV≥48 = 1.060, p = 0.002; ORPMV≥72 = 1.051, p = 0.011]. pRBC transfusion (ORPMV≥24 = 1.156, p = 0.001; ORPMV≥48 = 1.156, p < 0.001; ORPMV≥72 = 1.135, p ≤ 0.001) and PC transfusion (ORPMV≥24 = 1.366, p = 0.029; ORPMV≥48 = 1.226, p = 0.030; ORPMV≥72 = 1.229, p = 0.011) were independent risk factors for PMV. FFP had no noticeable effect on PMV [ORPMV≥48 = 0.999, 95% confidence interval (CI) 0.998–1.000, p = 0.039; ORPMV≥72 = 0.999, 95% CI: 0.998–1.000, p = 0.025].ConclusionsIn patients with ATAAD after TAR, the incidence of PMV was very high. Blood products transfusion was closely related to postoperative mechanical ventilation time. pRBC and PC transfusions and age increased the incidence of PMV at all three endpoints.

Wheat flour, the most important source of food globally, is also one of the most common causative agents of food allergy. Wheat gluten protein, which accounts for 80% of the total wheat protein, is a major determinant of important wheat-related disorders. In this study, the effects of Pediococcus acidilactici XZ31 against gluten-induced allergy were investigated in a mouse model. The oral administration of P. acidilactici XZ31 attenuated clinical and intestinal allergic responses in allergic mice. Further results showed that P. acidilactici XZ31 regulated Th1/Th2 immune balance toward Th1 polarization, which subsequently induced a reduction in gluten-specific IgE production. We also found that P. acidilactici XZ31 modulated gut microbiota homeostasis by balancing the Firmicutes/Bacteroidetes ratio and increasing bacterial diversity and the abundance of butyrate-producing bacteria. Specifically, the abundance of Firmicutes and Erysipelotrichaceae is positively correlated with concentrations of gluten-specific IgE and may act as a fecal biomarker for diagnosis. The evidence for the role of P. acidilactici XZ31 in alleviating gluten-induced allergic responses sheds light on the application of P. acidilactici XZ31 in treating wheat allergy.

The species of Kokeshia Miyamoto, 1960 from China are reviewed, K. hsiaoi Ren & Zheng, 1992 is redescribed, and ten new species from China are described. Among these new species, K. baii sp. nov., was collected from Hong Kong, and the other nine, K. acutiformis sp. nov., K. bui sp. nov., K. caii sp. nov., K. drepanoides sp. nov., K. hilli sp. nov., K. pengae sp. nov., K. redeii sp. nov., K. renae sp. nov. and K. weirauchae sp. nov., from Yunnan. Thus, the number of known species of this genus is increased from eight to eighteen. Photographs of the habitus, forewing, abdomen and genitalic structures; drawings and scanning electron micrographs of male abdomen and genitalic structures are provided. A key to all the species currently recognized in the genus Kokeshia is presented, a distribution map and checklist for all known species is provided.

Observed different evolutions of SST anomalies in the South China Sea (SCS) between super and other El Niño events are revealed. The results suggest that the first (second) warming peak is warmer than the second (first) one for super (other) El Niño composite. Mixed layer heat budget analysis indicates that during the first warming period, the vertical advection induced by the anomalous anticyclone in the SCS (SCSAC) warms the SST in the central SCS through the basin-scale downwelling motion for super El Niño composite. In contrast, the positive shortwave radiation anomalies from atmosphere into ocean associated with the reduced total cloud cover contribute the most warming SST for other El Niño composite. During the cooling period, the horizontal linear cold advection associated with a large west-east SST gradient anomaly and climatological western boundary currents plays the most important role in rapid cooling SST for super El Niño composite. However, the nonlinear warm advection associated with the northward current anomalies and anomalous SST gradient in the western SCS maintains the SCS SST for other El Niño composite. During the second warming period, the net surface heat flux anomalies act as a damping process, while the warming process could be attributed to the vertical warm advection. Specially, the SCSAC weakens but the anomalous easterly winds strengthen to suppress the upwelling off the coast of southeast Vietnam for other El Niño composite. The anomalous ocean circulations and associated advections are related to the development of SCSAC, which could be largely attributed to the SST anomalies in the Indian Ocean. In contrast with previous studies, our results highlight the role of ocean dynamics in the evolution of SCS SST anomalies during El Niño of different intensities.

Hyphalus shiyuensis sp. nov. is described from Xisha Islands of China, which represents the ninth species and provides new distribution information for this unique intertidal genus. Brief comparisons between the new species and the known species are given. An updated key to the species of genus Hyphalus is provided.

In deep underground engineering, understanding of time-related stress memory properties is critical to evaluate the in situ stress conditions of a rock mass. In this study, the time-sensitivity mechanism of the rock stress memory properties under tensile stress was investigated. It was found that the material property (Poisson's ratio) and crack angle were the controlling factors of the Kaiser effect (KE) under tensile stress. In particular, the time-sensitivity of the stress memory properties was closely related to the crack growth path. When the failure of the rock specimen was dominated by tensile microcracks and the crack development direction was deflected by up to 30° in the successive loading process, the stress memory capacity was likely to be time-independent for a sandstone specimen. The distribution of the Felicity ratio in a Brazilian test was more discrete than that in a three-point bending test. It also showed that the changes in the crack path, rather than the time interval between successive loading cycles, led to inaccuracy of the detected KE. This study provides insights into stress memory-related issues under uniaxial or more complex stress conditions and thus facilitates development of methods for testing in situ mechanical behaviors of rocks with acoustic emission (AE) technology.

Cellular markers of endothelial cell proliferation help identify the vasculature of tumours in homeostasis. Here, the authors report Apelin-based synthetic Notch (synNotch) receptors that can target Apj expressed on endothelium and stimulate the synNotch pathways upon Apj binding, and suggest this as a strategy to treat solid tumours.

Abstract Background Chilli veinal mottle virus (ChiVMV), which belongs to the genus Potyvirus of the family Potyviridae, mainly infects solanaceous plants and has caused serious economic losses in Asia and Africa. Tobacco plants infected with ChiVMV suffered from punctate necrosis of leaves, leaf deformation, systemic necrosis of leaves and stems, and eventually plant death. However, ChiVMV infection could not usually be identified given the lack of rapid and efficient detection assays in tobacco plants. Therefore, an isolate of tobacco-infecting ChiVMV (ChiVMV-LZ) was obtained, and a novel isothermal amplification and detection technique, reverse transcription-recombinase polymerase amplification (RT-RPA), was established to detect ChiVMV in tobacco plants. Methods In this study, the full-length genome of ChiVMV-LZ was obtained using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) assays. The genome sequence of ChiVMV-LZ was characterized by sequence alignment and phylogenetic analysis. Then, a RT-RPA assay was established for rapid and sensitive detection of ChiVMV-LZ in tobacco. Additionally, the established RT-RPA assay was compared to the RT-PCR assay in aspect of sensitivity and application in field-collected tobacco samples. Results ChiVMV-LZ was isolated from diseased tobacco in Luzhou, Sichuan, China. The tobacco plants inoculated with ChiVMV-LZ showed typical symptoms of yellow and round spots on the leaves, and curled and folded leaf margin, similar to those observed on naturally ChiVMV-infected tobacco in the field. The full-length genomic sequence of ChiVMV-LZ was determined to be 9742 nucleotides. Sequence alignment and phylogenetic analysis showed that ChiVMV-LZ was most closely related to ChiVMV-Yp8 isolated from pepper plants in Sichuan province while distantly related to ChiVMV-YN from tobacco in Yunnan province, indicating a possibly geographical differentiation of ChiVMV isolates. Additionally, a RT-RPA assay was established for rapid detection of ChiVMV in tobacco. The RT-RPA has no cross-reaction with other related tobacco viruses and is about 10-fold more sensitive than conventional RT-PCR method. Conclusion The characterization of ChiVMV-LZ infecting tobacco was determined, and the established RT-RPA assay provides a reliable and effective method for rapid detection of ChiVMV in tobacco.

The extent of fracture development is associated with the degree of enrichment of a natural gas reservoir and its productivity. Based on numerical simulation results of the paleotectonic stress field, a set of evaluation methods for determining the degree of development of reservoir tectonic fractures were established using rock rupture criteria. Taking the fourth member of the Leikoupo Formation in the Pengzhou area of western Sichuan as an example, a finite element (FE) method was employed to simulate the paleo-tectonic stress field during the period of fracture development, and the degree of tectonic fracture development was further evaluated using the above methods. The results indicated that effective fractures were created in the Himalayan period. In this time, mainly NE–NEE and nearly E–W strike tectonic fractures were developed in the target layer. The fractures were mainly low-angle and oblique fractures, while the high-angle fractures were less developed. According to the integrative fracture index (F), five typical fracture development areas were determined: the fault zone, and the northern, eastern, southeastern, and central regions of the study area. The reliability of the fracture prediction results was verified using fracture distribution statistics and gas production test results.

Previous studies showed that brain trauma promotes repair of peripheral nerve injury by reducing scar in nerve endings. The effect of brain injury at different locations on ipsilateral rat sciatic nerve regeneration in Sprague-Dawley rats was found to promote the repair of ipsilateral sciatic nerve injury, thus providing further experimental evidence for the unveiling of traumatic brain injury promoting peripheral nerve regeneration.

Microplastics (MPs) are rapidly colonized by microbial biofilms in a natural aquatic environment, and the nature of the microbial community and type of MP can result in different degradation products of organic pollutants. Here, we quantified the degradation products of a ubiquitously detected pollutant, decabrominated diphenyl ether (BDE-209), under both light-only and biota conditions and in the absence or presence of three kinds of MPs, styrofoam polystyrene, hard polyamide, and polypropylene film. The results showed that the BDE-209 concentration increased by 0.7–2.8 fold in the presence of MPs, probably due to the “sustained release” desorption effect. Under light-only conditions, the penta- and hexa-BDE concentrations in the presence of styrofoam or hard MPs were significantly reduced, which can be deemed a beneficial effect. However, when biota were present, the debromination products increased with the addition of MPs, particularly in the presence of styrofoam MPs. These products caused a 1.7-fold upregulation in triiodothyronine content and a 5.9-fold upregulation of thyroid stimulating hormone β expression in zebrafish larvae. The increase in debromination products could be attributed to the distinct high abundance of the bacteria Chloroflexi, Proteobacteria, and Basidiomycotina on styrofoam MPs that can participate in pollutant degradation. Collectively, our results indicate that MPs can alter the degradation pathways of BDE-209 and increase the toxicity to the endocrine system and the thyroid in aquatic organisms.

This study reveals the features of the strong intraseasonal variability (ISV) of the upper-layer current in the northern South China Sea (NSCS) based on four long-time mooring observations and altimeter data. The ISV of the upper-layer current in the NSCS consists of two dominant periods of 10–65 days and 65–110 days. The ISV with period of 10–65 days is much strong in the Luzon Strait and decays rapidly westward along the slope. The ISV with the period of 65–110 days is relatively strong along the slope with two high cores at 115 and 119°E, whereas it is weak in the Luzon Strait. The 10–65-day ISV can propagate directly from the western Pacific into the NSCS for most of the time. However, due to its long wavelength, the 65–110-day ISV propagates into the NSCS indirectly, possibly similar to the wave diffraction phenomenon. The spatial differences between the two main frequency bands are primarily due to the baroclinic and barotropic instabilities. The spatial distribution of the upper-layer ISV is closely associated with the mesoscale eddy radius of the NSCS. The eddy radius is directly proportional to the strength of 65–110-day ISV, but it is inversely proportional to the strength of 10–65-day ISV.

Abnormal expression of the transcription factor Y-box-binding protein-1 (YBX1) is associated with the proliferation, migration, aggressiveness, and stem-like properties of various cancers. These characteristics contribute to the tumorigenesis and metastasis of cancer. We found that the expression levels of Mucin-1 (MUC1) and YBX1 were positively correlated in lung adenocarcinoma cells and lung adenocarcinoma tissue. Our retrospective cohort study of 176 lung adenocarcinoma patients after surgery showed that low expression of both YBX1 and MUC1 was an independent predictor of the prognosis and recurrence of lung adenocarcinoma. In lung adenocarcinoma cells, the silencing/overexpression of YBX1 caused a simultaneous change in MUC1, and MUC1 overexpression partially reversed the decreased tumor cell migration, aggressiveness, and stemness caused by YBX1 silencing. Moreover, chromatin immunoprecipitation (ChIP) and dual-luciferase reporter assays proved that MUC1 was the downstream target of YBX1 and that YBX1 bound to the -1480~-1476 position in the promoter region of MUC1 to regulate its transcription. Furthermore, in mouse xenograft models and a lung cancer metastasis model, MUC1, which is downstream of YBX1, partially reversed the decreased number and size of tumors caused by YBX1 silencing. In conclusion, our findings indicated a novel mechanism by which YBX1 promotes the stemness and metastasis of lung adenocarcinoma by targeting MUC1 and provided a combination approach for diagnosis different from traditional single tumor biomarkers to predict patient prognosis and provide clinical treatment targets.

Fam20C, a typical member of Fam20 family, has been well-known as a Golgi casein kinase, which is closely associated with Raine Syndrome (RS). It can phosphorylate many secreted proteins and multiple substrates, and thereby plays a crucial role in biological functions. More importantly, Fam20C has also been found to enhance the metastasis of several types of human cancers, such as breast cancer, indicating that Fam20C may be a promising therapeutic target. Accordingly, some small-molecule inhibitors of Fam20C have been reported in cancer. Taken together, these inspiring findings would shed new light on exploiting Fam20C as a potential therapeutic target and inhibiting Fam20C with small-molecule compounds would provide a clue on discovery of more candidate small-molecule drugs for fighting with human diseases.

Hearing loss is one of the most common disabilities affecting both children and adults worldwide. However, traditional treatment of hearing loss has some limitations, particularly in terms of drug delivery system as well as diagnosis of ear imaging. The blood–labyrinth barrier (BLB), the barrier between the vasculature and fluids of the inner ear, restricts entry of most blood-borne compounds into inner ear tissues. Nanoparticles (NPs) have been demonstrated to have high biocompatibility, good degradation, and simple synthesis in the process of diagnosis and treatment, which are promising for medical applications in hearing loss. Although previous studies have shown that NPs have promising applications in the field of inner ear diseases, there is still a gap between biological research and clinical application. In this paper, we aim to summarize developments and challenges of NPs in diagnostics and treatment of hearing loss in recent years. This review may be useful to raise otology researchers’ awareness of effect of NPs on hearing diagnosis and treatment.

Numerous studies have focused on the mechanical properties of shale specimens to support hydraulic fracturing in shale gas engineering. However, the failure modes of shale specimens containing a pre-existing flaw are still not clear. A series of uniaxial compression tests were conducted on shale specimens containing a pre-existing flaw coupled with acoustic emission (AE) technology and a high-speed camera. A slow enough and variable loading speed scheme (0–50 kN: 0.1 kN/s, 50–100 kN: 0.05 kN/s; 100-failure: 0.02 kN/s) is adopted to allow microcracks to fully develop. The damage mechanism in the mesoscale from moment tensor inversion of the AE signals and the cracking behaviors on the macroscale were associated. Both the pre-existing flaw and the bedding layers had significant influences on the mechanical characteristics, progressive cracking, and failure modes of the shale specimens. The peak stress increases fluctuated as the bedding layer angle α increases, and the maximum and minimum values take place at α=90° and α=0°, respectively. In addition, the pre-existing flaw, the bedding layers, and the compression stress field jointly control the failure modes of the shale specimens. It was found that the α=0° specimen failed due to tension splitting, the α=30° specimen failed due to tension splitting and shear across the matrix, the α=60 specimen failed due to shear across the shale matrix and shear along the bedding layers, and the α=90° specimen failed due to tension along the bedding layers and shear across the shale matrix. The study can provide some references to the construction of complex crack networks in shale gas engineering.

Natural products are well-characterized to have pharmacological or biological activities that can be of therapeutic benefits for cancer therapy, which also provide an important source of inspiration for discovery of potential novel small-molecule drugs. In the past three decades, accumulating evidence has revealed that natural products can modulate a series of key autophagic signaling pathways and display therapeutic effects in different types of human cancers. In this review, we focus on summarizing some representative natural active compounds, mainly including curcumin, resveratrol, paclitaxel, Bufalin, and Ursolic acid that may ultimately trigger cancer cell death through the regulation of some key autophagic signaling pathways, such as RAS-RAF-MEK-ERK, PI3K-AKT-mTOR, AMPK, ULK1, Beclin-1, Atg5 and p53. Taken together, these inspiring findings would shed light on exploiting more natural compounds as candidate small-molecule drugs, by targeting the crucial pathways of autophagy for the future cancer therapy.

Objective: To explore the relationship between dietary inflammatory index (DII) and heart failure (HF) in participants with cardiovascular and cerebrovascular diseases.Methods: NHANES (1998–2018) data were collected and used to assess the association of HF with DII. Twenty-four-hour dietary consumptions were used to calculate the scores of DII. Demographic characteristics and physical and laboratory examinations were collected for the comparison between HF and non-HF groups. Logistic regression analysis and random forest analysis were performed to calculate the odds rate and determine the potential beneficial dietary components in HF.Results: A total of 19,067 cardiac-cerebral vascular disease participants were categorized as HF (n = 1,382; 7.25%) and non-HF (n = 17,685; 92.75%) groups. Heart failure participants had higher levels of DII score compared with those in the non-HF group (0.239 ± 1.702 vs. −0.145 ± 1.704, p < 0.001). Compared with individuals with T1 (DII: −3.884 to −0.570) of DII, those in T3 (DII: 1.019 to 4.598) had a higher level of total cholesterol (4.49 ± 1.16 vs. 4.75 ± 1.28 mmol/L, p < 0.01), globulin (29.92 ± 5.37 vs. 31.29 ± 5.84 g/L, p < 0.001), and pulse rate (69.90 ± 12.22 vs. 72.22 ± 12.77, p < 0.001) and lower levels of albumin (40.76 ± 3.52 vs. 39.86 ± 3.83 g/L, p < 0.001), hemoglobin (13.76 ± 1.65 vs. 13.46 ± 1.77 g/dl, p < 0.05), and hematocrit (40.83 ± 4.69 vs. 40.17 ± 5.01%, p < 0.05). The odds rates of HF for DII from the logistic regression were 1.140, 1.158, and 1.110 in models 1, 2, and 3, respectively. In addition, from the results of random forest analysis, dietary magnesium, fiber, and beta carotene may be essential in HF.Conclusion: Dietary inflammatory index was positively associated with HF in US adults, and dietary intervention might be a promising method in the therapy of HF.

High-frequency motions in the southeastern South China Sea (SCS) have rarely been investigated due to sparse field observations. The vertical distribution and temporal variation of internal tides (ITs) and near-inertial waves (NIWs) near the Nansha area in the southeastern SCS were studied using a mooring current dataset from December 2018 to June 2019 in this study. Results showed that ITs were mainly dominated by O1, K1, and M2. Tidal energy analysis indicates that the diurnal ITs were the most energetic components, followed by the semidiurnal ITs. Modal decomposition reveals that diurnal ITs were dominated by mode-3, rather than mode-1, as reported by previous studies. The horizontal kinetic energy (HKE) of diurnal ITs fluctuated within a limited range, almost unaffected by the background field. However, the HKE of semidiurnal ITs was dominated by mode-1 and more affected by the background field, especially at the end of March. Most observations showed the phase of the NIWs propagating upward and the energy propagating downward. During the northeast monsoon period, the near-inertial energy had a large magnitude due to strong wind forcing. In addition, the near-inertial energy peaked from the middle of March to the beginning of April because of the input of NIWs from afar. Overall, near-inertial energy was found concentrated above a 500–600 m depth in the southeastern SCS.

Earthquakes are one of the most serious natural disasters, threatening ecological balance and security. Ecosystem services (ESs) reflect the multiple functions of an ecosystem. However, based on catastrophic disasters, the contributions of various factors to ESs recovery have not been previously quantified. We follow recovery after the 2008 Wenchuan earthquake to gain insight into these processes. To quantify ESs, biophysical models were applied, including scenario simulation and Geodetector methods that were used to explore the driving forces. We concluded as follows: in overall study area, ESs showed an insignificant but undulating increase from 2001 to 2017, indicating the earthquake had slight but long-term effects. In the vegetated areas denuded by co-seismic landslides, the loss in ESs was twice that in non-denuded areas, and the damaged ESs did not recover to pre-earthquake levels by 2017. Overall, climate change was the main driving force, and the factors directly related to local climate had a more obvious impact on ESs especially elevation and geomorphic types. More than three quarters of study area showed ESs increases, with climate (accounting for 40.26%) and its multiple interactions with non-climate factors (accounting for 45.91%) contributed the most to ESs restoration, respectively at low elevations and in the subalpine mountains. Nearly a quarter of the study area was still degraded, of which the proportion of the area representing non-climate-driven factors had increased (accounting for 20.68%). The intensity of ESs degradation mainly concentrated in XI and X regions where co-seismic landslides were dense, which was one of the most influential non-climate factors. We therefore recommend that in the post-disaster reconstruction stage, more attention should be paid to restoration and maintenance of ecological service functions in areas with frequent secondary disasters, so as to maximize the benefits of post-disaster reconstruction projects and seek socio-economic and ecological environment collaborative development.

Alternative splicing (AS) is a key mechanism involved in regulating gene expression and is closely related to tumorigenesis. The incidence of thyroid cancer (THCA) has increased during the past decade, and the role of AS in THCA is still unclear. Here, we used TCGA and to generate AS maps in patients with THCA. Univariate analysis revealed 825 AS events related to the survival of THCA. Five prognostic models of AA, AD, AT, ES, and ME events were obtained through lasso and multivariate analyses, and the final prediction model was established by integrating all the AS events in the five prediction models. Kaplan–Meier survival analysis revealed that the overall survival rate of patients in the high-risk group was significantly shorter than that of patients in the low-risk group. The ROC results revealed that the prognostic capabilities of each model at 3, 5, and 8 years were all greater than 0.7, and the final prognostic capabilities of the models were all greater than 0.9. By reviewing other databases and utilizing qPCR, we verified the established THCA gene model. In addition, gene set enrichment analysis showed that abnormal AS events might play key roles in tumor development and progression of THCA by participating in changes in molecular structure, homeostasis of the cell environment and in cell energy. Finally, a splicing correlation network was established to reveal the potential regulatory patterns between the predicted splicing factors and AS event candidates. In summary, AS should be considered an important prognostic indicator of THCA. Our results will help to elucidate the underlying mechanism of AS in the process of THCA tumorigenesis and broaden the prognostic and clinical application of molecular targeted therapy for THCA.

High-alkali species in coal are notorious for causing ash slagging and fouling incidents. In this paper, four high-alkali coals were individually subject to hydrothermal pretreatment (HTP), within a batch-type autoclave at 300 °C for 1 h, and the treated coals were analyzed, along with the oxygen-containing functional groups determined by Fourier transform infrared spectrometer (FT-IR). Then the alkali species and other components in the coal ash were quantified by X-ray fluorescence (XRF) for evaluating the ash slagging and fouling tendency. Apart from this, FactSage was adopted to simulate the occurrence and transformation of alkali species during coal thermal conversion ending at various temperatures. The findings indicate that the treated coals are superior to the parent ones in terms of certain remarkable changes via HTP. The moisture, oxygen and sulfur of the hydrothermally treated coals decline obviously, while the calorific value rises sharply. HTP could reduce the alkali species to less than 2% (%, by weight, equivalent to Na2O in dry ash), with a maximum removal ratio of 88.9%, lowering the ash slagging and fouling tendency. The proposed mechanism of HTP was that the alkali species in coal matrix became released due to the breakage of the coal functional groups and micropores during HTP. Keywords: Hydrothermal pretreatment, High-alkali coal, Ash slagging and fouling tendency, Lowering, FactSage

Magnetically separable mesoporous activated carbon was prepared from brown coal in the presence of Fe3O4 as a bi-functional additive. Magnetic activated carbon (MAC) was characterized by low-temperature nitrogen adsorption, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and vibrating sample magnetometry (VSM). The evolution behaviors and transition mechanism of Fe3O4 during the preparation of MAC were investigated. The results show that prepared MAC with 6 wt% Fe3O4 addition having a specific surface area and mesopore ratio of 370 m2·g−1 and 55.7%, which meet the requirements of adsorption application and magnetic recovery. Highly dispersed iron-containing aggregates with the size of 0.1 μm in the MAC were observed. During the preparation of MAC, Fe3O4 could enhance the escape of volatiles during the carbonization. Fe3O4 could also accelerate burning off the carbon wall during activation, which leads to enlarging micropore size, then resulting in the generation of mesopore and macropore. As a result, a part of Fe3O4 converted into FeO, FeOOH, α-Fe, γ-Fe, Fe2SiO4 and compound of Aluminum-iron-silicon. The prepared activated carbon, which was magnetized by both of residual Fe3O4, reduced α-Fe and γ-Fe, can be easily separated from the original solution by external magnetic field. Keywords: Magnetic activated carbon, Preparation, Brown coal, Ferroferric oxide, Magnetic separation

High nitrogen steel (HNS) has an excellent tensile strength and impact property due to the solid solution strengthening of nitrogen, which provides a good application prospect in many fields. Fusion welding is one of the main processing methods of HNS, but the process is prone to spatters, serious nitrogen losses, N2 porosities, and poor performances of joints, resulting in the failure of large-scale engineering applications of HNS. In this work, the development of HNS welding is reviewed, and the problems including the droplet transfer instability, N2 porosities, nitrogen losses and poor mechanical properties due to high nitrogen content are discussed. According to previous welding experiences, the adoption of a welding method with a low heat input is proposed, which utilizes powders instead of wires, optimizes compositions in the shielding gas and feeding materials in order to solve the above problems, and improves the mechanical properties of the weld.

To evaluate the seismic performances of the ultra-high voltage (UHV) gas-insulated switchgear (GIS) bushings made by porcelain and glass fiber reinforced polymer (GFRP) composite materials, shaking table tests were conducted on the two full-scale GIS bushings. The dynamic characteristics and seismic responses of the two UHV GIS bushings were obtained. The experimental results indicated that the two UHV GIS bushings meet the seismic requirements in the corresponding standards. The supporting frame and bus canister amplify the seismic responses of the UHV GIS bushings. Under earthquakes, the GFRP composite UHV GIS bushing is safer than the porcelain bushing. In the seismic design of the electrical substation, the large seismic displacement of the GFRP composite UHV GIS bushings should be considered.

Multiple studies have uncovered the pivotal role of gut microbiota in the development of food allergy. However, the effects of gut microbiota on peanut allergy are still unclear. Here, we characterized the gut microbiota composition of peanut-allergic mice by 16S rRNA sequencing and analyzed the correlation between allergic indicators and gut microbiota composition. Outcomes showed that the gut microbiota composition was reshaped in peanut-allergic mice, with Acidobacteriota, Lachnospiraceae, Rikenellaceae, Alistipes, Lachnospiraceae_NK4A136_group significantly down-regulated and Muribaculaceae up-regulated. All of them were significantly correlated with the serum peanut-specific antibodies. These results suggested that these six bacterial OTUs might be the gut microbial signatures associated with peanut allergy.

The theory of the RNA world, especially with the catalytic capability of RNA, provides a reasonable framework explaining the evolution of molecular genetics system before the scenario of the central dogma. However, it remains a challenge to deduce the origin mechanism of rRNAs. Here we reconstructed the phylogenetic relationships of archaea and bacteria with bootstrap values of most nodes, especially the deep ones, higher than 90%. Based on the well-resolved tree, the full lengths of 16S, 5S, and 23S rRNA sequences of the last universal common ancestor (LUCA) were reconstructed for the first time. The potential similarities shared by the three ancestral rRNA sequences were further explored by searching for repeat short fragments in the level of purine–pyrimidine (RY) with certain lengths and arrangements. With the lengths ranging from 2 to 14, functional short fragments could be found in the three RNAs. As a representative, a set with a total of 75 short fragments of 11 nucleotides in length can recover all types of the known functional sites of ribosomes in a most concise manner. The 75 short fragments cluster around the functional center of the ribosome, among which 18 of them are highly conserved across five or six kingdoms and still contain all types of known functional sites except one. Alternatively, according to the strategy using the level of AUGC instead of RY, a similar pattern can be recovered. Such results indicate the local similarities shared by 16S, 5S, and 23S rRNAs and thus suggest a possible general mechanism in the formation of the LUCA rRNAs.

Rock failure and instability usually lead to significant engineering disasters. This paper aims to establish an experimental failure criterion to predict rock failure via testing the laser vibration characteristics of marble specimens. Uniaxial compression tests and Brazilian tests were carried out on marble specimens coupled with acoustic emission technology and laser Doppler vibrometry measurement technology. The whole laser vibration waveform of the marble specimen was divided into elastic stage, plastic stage, and failure stage. Although different frequency spectrum characteristics were identified in different waveform phases, a wide frequency spectrum was always present prior to rock failure. Furthermore, the wide frequency band frequency spectra characteristics took place 30.9 s and 21.3 s earlier than the rapid increase of the acoustic emission counts in the uniaxial compression test and Brazilian test, respectively. Taking the wide frequency spectrum as a failure criterion for the failure of loaded marble is quick, convenient, and reasonable. Using laser Doppler vibrometry measurement has the advantages of being remote, non-contacting, and earlier warning. This research can provide a reference for the further study of forecasting rock failure.

This work concentrates on the energy consumption and grinding energy efficiency of a laboratory vertical roller mill (VRM) under various operating parameters. For design of experiments (DOE), the response surface method (RSM) was employed with the VRM experiments to systematically investigate the influence of operating parameters on the energy consumption and grinding energy efficiency. The prediction models of energy consumption (Ecs) and grinding energy efficiency (η) were established respectively with the operating parameters (loading pressure, rotation speed and moisture content). Analysis of variance (ANOVA) was performed to obtain useful knowledge in designing operating parameters. Moreover, the multi-objective optimization design (MOD) method was conducted to seek out the optimal parameters of the VRM, and a set of optimal parameters was gained based on the desirability approach by Design-Expert. It is proved that the optimized prediction results match the experimental results well, which indicates this research offers a reliable guidance for reducing energy consumption and improving grinding energy efficiency.

Objective In birds, three types of melatonin receptors (MTNR1A, MTNR1B, and MTNR1C) have been cloned. Previous researches have showed that three melatonin receptors played an essential role in reproduction and ovarian physiology. However, the association of polymorphisms of the three receptors with duck reproduction traits and egg quality traits is still unknown. In this test, we chose MTNR1A, MTNR1B, and MTNR1C as candidate genes to detect novel sequence polymorphism and analyze their association with egg production traits in Shaoxing duck, and detected their mRNA expression level in ovaries. Methods In this study, a total of 785 duck blood samples were collected to investigate the association of melatonin receptor genes with egg production traits and egg quality traits using a direct sequencing method. And 6 ducks representing two groups (3 of each) according to the age at first eggs (at 128 days of age or after 150 days of age) were carefully selected for quantitative real-time polymerase chain reaction. Results Seven novel polymorphisms (MTNR1A: g. 268C>T, MTNR1B: g. 41C>T, and g. 161T>C, MTNR1C: g. 10C>T, g. 24A>G, g. 108C>T, g. 363 T>C) were detected. The single nucleotide polymorphism (SNP) of MTNR1A (g. 268C>T) was significantly linked with the age at first egg (pT and egg production traits: total egg numbers at 34 weeks old of age and age at first egg. In addition, the mRNA expression level of MTNR1A in ovary was significantly higher in late-mature group than in early-mature group, while MTNR1C showed a contrary tendency (p

Abstract Thirty conductivity-temperature-depth profiler casts in the Challenger Deep were conducted during four cruises from December 2015 to February 2017. Two cruises took place in the summer, and two in the winter. The results demonstrated that water characteristics varied seasonally. The temperature minimum values were the same between the four cruises, but its depth was noticeably shallower in the winter than that in the summer. The θ-S diagram indicated that deep water is more saline in the summer than that in winter at the same potential temperature. Mixing is more intense between 5000 and 6800 m in the summer than that in the winter. The dissipation rate and eddy diffusivity vertically averaged between 5000 and 6800 m in the summer were ε T = 3.277 × 10−8 m 2 s −3 and K zT = 2.58 × 10−2 m 2 s −1, respectively. The geostrophic flows below the reference level of 3000 dbar were cyclonic in the summer, travelling westwards in the northern and eastwards in the southern areas of the Challenger Deep.

Abstract Water–sand flow triggered by rainfall is the dominant mechanism for instability and failure of sand slopes. To further analyze the stability state of sand on a slope under different rainfall conditions, the initiation conditions and flow characteristics of water–sand flows are studied. Based on the theory of equilibrium forces and hydrological dynamics, a 1:100-scale analog model is built and verified with field observation data. The results indicate three dynamic stabilization stages of the sand slope under different weather conditions: dry sand, wet sand, and water–sand flow. Water–sand flows are triggered easily under short duration and heavy rainfall conditions. The rainfall threshold required to initiate water–sand flow is 4.14 mm/h. Rainfall amount and duration required to initiate water–sand flow decrease with fine sand content increasing. A sand head that develops at the front of the water–sand flow results in a flow along the edge of the sand debris flow and a “tree root” flow morphology. Modeling results are consistent with theoretical analysis and field observations.

Rotary drums are widely used in numerous processes in industry to handle granular materials. In present work, heat transfer processes in drums with L-shaped lifters have been investigated by coupling the discrete element method (DEM) with heat transfer model. Effects of both operational and structural parameters have been analyzed. It is found that increasing rotational speed could improve heat transfer to a certain extent, however, just in relatively low speed stage. When lifter number increases, the heat transfer speed slightly decreases. An increasing lifter height could promote heat transfer first and then reduces it, but the amplitude of variation keeps small. The heat transfer rate descends with increasing lifter width. The heat transfer mechanisms have also been discussed by comparing mixing rates, total contact areas for thermal conduction, time constants (TC) indicating apparent heat transfer rate and effective heat transfer coefficients(HTC). It is concluded that dynamic conduction due to particle flow is dominated in all cases. The L-shaped lifers are turned out not a good choice when heat conduction between particles is prominent.

A recent study by Xie and colleagues show the dynamic behavior of the catenary system in turbulent and uniform flow conditions under four different tension combinations (Xie et al., 2018). The data presented in this article include the maximum displacement and maximum acceleration of the hanging point along the wind direction, comparing the values in two different wind flows and four different tension combinations. These data are useful to explore the influence and reveal the resonance interaction between structural elements and contact wire.

Disconnect switches, the widely used electrical substation apparatuses, however, were severely damaged in several earthquakes, causing huge economic losses. Disconnect switches with higher voltage are more vulnerable to earthquake for their great height and flexibility. The interaction between posts and blades might magnify the seismic responses, which is not fully understood and deserves further investigations. Therefore, a full-scale shake table test on a new-style latticed ±800 kV ultra-high voltage direct current disconnect switch was carried out to evaluate its dynamic properties and seismic responses. Two different operational conditions with the main blade open and closed were tested, respectively. Interaction between posts and blades was studied via acceleration amplification factors and the spectrum analysis. The test result indicates interaction only exists in the length direction, and will amplify seismic responses of the post with the main blade, while reduce those of the post with the fixed contact. Moreover, the seismic responses of specimen are smaller than those in previous studies, suggesting the latticed structural form alleviate the negative effects induced by the great height and flexibility, and is helpful for structural design of disconnect switches.

The formation process of EDZs (excavation damaged zones) in the roadways of deep underground mines is complex, and this process is affected by blasting disturbances, engineering excavation unloading, and adjustment of field stress. The range of an excavation damaged zone (EDZ) changes as the time and space change. These changes bring more difficulties in analyzing the stability of the surrounding rock in deep engineering and determining a reasonable support scheme. In a layered rock mass, the distribution of EDZs is more difficult to identify. In this study, an ultrasonic velocity detector in the surrounding rock was used to monitor the range of EDZs in a deep roadway which was buried in a layered rock mass with a dip angle of 20–30°. The space-time distribution laws of the range of EDZs during the excavation process of the roadway were analyzed. The monitoring results showed that the formation of an EDZ can be divided into the following stages: (1) the EDZ forms immediately after the roadway excavation, which accounts for approximately 82%–95% of all EDZs. The main factors that affect the EDZ are the blasting load, the excavation unloading, and the stress adjustment; (2) as the roadway excavation continues, the range of the EDZs increases because of the blasting excavation and stress adjustment; (3) the later excavation zone has a comparatively larger EDZ value; and (4) an asymmetric supporting technology is necessary to ensure the stability of roadways buried in layered rocks. Additionally, the predictive capability of random forest modeling is evaluated for estimating the EDZ. The root-mean-square error (RMSE) and mean absolute error (MAE) are used as reliable indicators to validate the model. The results indicate that the random forest model has good prediction capability (RMSE = 0.1613 and MAE = 0.1402).

In this study, M2 tidal energy and tide-induced mixing in the Mariana double ridges are investigated with a high-resolution three-dimensional non-hydrostatic numerical model and baroclinic energy budget analysis. The interference effect of the double ridges on the internal tide in the Mariana is examined by omitting either the eastern or the western ridge. Our results show that the baroclinic velocity on the sides of the interior facing slopes of the double ridges is larger than that on the other sides. In the double ridges, high values of dissipation reaching O (10−6 W kg−1) are accompanied by diapycnal diffusivity reaching O (10−1 m2 s−1), which is several orders of magnitude higher than the mixing of the open ocean. The bottom diapycnal mixing in the inner region between the two ridges is one order of magnitude larger than the mixing outside the ridges, indicating the important role of the interference of the double-ridge topography on the mixing in the Mariana Arc. Omitting either the eastern or the western ridge would have a significant impact on tide current, baroclinic energy flux and dissipation, and diapycnal mixing. The internal tide conversion, dissipation, and flux divergence are amplified by the double ridge topography, especially in the central part of the double ridges. Through energy budgets analysis, we conclude that the eastern ridge is the main source of the baroclinic tide in the Mariana double ridges.

Ice accretion can cause problems on polar ships, ocean platforms, and in other marine industries. It is important to understand the interface debonding behavior between ice and the surface of equipment. In this work, we created a mechanical model to analyze the interface debonding behavior between a square-based ice cuboid and an elastic coating base, using contact mechanics and fracture mechanics. Three-dimensional (3D) finite element (FE) simulation was used to simulate the interface debonding for normal and shear separation. A bilinear cohesive zone model (CZM) was used to simulate the interface between the ice cuboid and the elastic coating. We investigated the effect of the elastic modulus E of an elastic film on the critical detachment force Fc for normal and shear separation. The results showed that Fc increases with an increase of the elastic modulus of the elastic film. When E exceeds a certain level, Fc achieves a constant value and then remains stable. Finally, a series of epoxy/polydimethylsiloxane (PDMS) interpenetrating polymer-network (IPN) gel coatings with different elastic moduli were prepared. The ice tensile and shear adhesion strengths (σice and τice) of the coatings were measured. The results were roughly consistent with the results of the numerical simulation when E < 1 MPa.

In this work, isotropic pitch precursors are synthesized by the bromination-debromination method with ethylene bottom oil (EO) as the raw material and bromine as the initiator for pitch formation and condensation reactions. The aggregation structure, molecular weight distribution, and molecular structure of isotropic pitch precursors are characterized by thermal mechanical analyzer (TMA), MALDI TOF-MS, and 13C NMR, respectively, for revealing the mechanism of synthesis of isotropic pitch precursors. The results show that at low bromine concentrations, polycyclic aromatic hydrocarbons (PAHs) were mainly ordered in cross-linked structures by bromination-debromination through substitution reactions of side chains. The condensed reactivity can be improved by the effect of bromine, meaning that condensation reaction was aggravated by the method of bromination-dehydrobromination. In the presence of excess bromine, the cross-linked stereo structure of PAHs changed to the planar structure of condensed PAHs, which was not conducive to the subsequent spinning and preparation of carbon fibers.

Objective: To investigate the protective effect of erythropoietin (EPO) pretreatment on ischemic acute renal failure in rats and its molecular mechanism. Methods: Male Sprague–Dawley rats were selected as experimental animals and they were randomly divided into the sham operation group (sham group), ischemia-reperfusion injury group (IRI group) and EPO pretreatment group (EPO group). Each group had 15 rats. Serum specimens and renal specimens were collected after a IRI model was built for 4, 12 and 24 h. The contents of creatinine, urea nitrogen tumor necrosis factor-alpha (TNF-a), interleukin-1 (IL-1), IL-6 and IL-8 in serum and the contents of TNF-a, IL- 1, IL-6, IL-8, toll like receptor 4 (TLR4) and nuclear factor-kappa B (NF-kB) in the kidney tissue were determined. Results: After 4, 12 and 24 h reperfusion, there were differences between the contents of creatinine, urea nitrogen TNF-a, IL-1, IL-6 and IL-8 in serum and the contents of TNF-a, IL-1, IL-6, IL-8, TLR4 and NF-kB in rats of the three groups (P < 0.05). The contents of creatinine, urea nitrogen TNF-a, IL-1, IL-6 and IL-8 in serum and the contents of TNF-a, IL-1, IL-6, IL-8, TLR4 and NF-kB in the kidney tissue in rats of the IRI group were significantly higher than those of the sham group; and the contents of creatinine, urea nitrogen TNF-a, IL-1, IL-6 and IL-8 in serum and the contents of TNF-a, IL-1, IL-6, IL- 8, TLR4 and NF-kB in the kidney tissue in rats of the EPO group were distinctly lower than those of the IRI group. Conclusions: EPO pretreatment can protect the renal function of rats with ischemic acute renal failure by inhibiting the TLR4/NF-kB pathway mediated inflammatory responses.

High grade mucinous urothelial carcinoma is a rare pathological variant. There is still controversy as to its nomenclature and classification. We report the case of a 64 year old female with history of pelvic pain who was incidentally discovered to have a left upper pole renal mass. Left nephroureterectomy was performed and histopathological examination revealed high grade mucinous urothelial carcinoma. Accurate diagnosis of this distinct pathological entity will allow for better understanding of phenotypic behavior and inform best treatment strategies.

An effective deicing system is needed to be designed to conveniently remove ice from the surfaces of structures. In this paper, an ultrasonic deicing system for different configurations was estimated and verified based on finite element simulations. The research focused on deicing efficiency factor (DEF) discussions, prediction, and validations. Firstly, seven different configurations of Lead zirconate titanate (PZT) disk actuators with the same volume but different radius and thickness were adopted to conduct harmonic analysis. The effects of PZT shape on shear stresses and optimal frequencies were obtained. Simultaneously, the average shear stresses at the ice/substrate interface and total energy density needed for deicing were calculated. Then, a coefficient named deicing efficiency factor (DEF) was proposed to estimate deicing efficiency. Based on these results, the optimized configuration and deicing frequency are given. Furthermore, four different icing cases for the optimize configuration were studied to further verify the rationality of DEF. The effects of shear stress distributions on deicing efficiency were also analyzed. At same time, a cohesive zone model (CZM) was introduced to describe interface behavior of the plate and ice layer. Standard-explicit co-simulation was utilized to model the wave propagation and ice layer delamination process. Finally, the deicing experiments were carried out to validate the feasibility and correctness of the deicing system.

The sequences of 18S and 28S rDNAs have been used as molecular markers to resolve phylogenetic relationships of Heteroptera for two decades. The complete sequences of 18S rDNAs have been used in many studies, while in most studies only partial sequences of 28S rDNAs have been used due to technical difficulties of amplifying the complete lengths. In this study, we amplified the complete 18S and 28S rDNA sequences of Eurydema maracandica Oshanin, 1871, and reconstructed the secondary structure models of the corresponding rRNAs. In addition, and more importantly, all of the length variable regions of 18S rRNA were compared among 37 families of Heteroptera based on 140 sequences, and the D3 region of 28S rRNA was compared among 51 families based on 84 sequences. It was found that 8 length variable regions could potentially serve as molecular synapomorphies for some monophyletic groups. Therefore discoveries of more molecular synapomorphies for specific clades can be anticipated from amplification of complete 18S and 28S rDNAs of more representatives of Heteroptera.

Data from a subsurface mooring deployed in the western South China Sea shows clear intra-seasonal oscillations (ISO) at the period of 40~70 days. Analysis of remotelysensed sea surface height (SSH) anomalies in the same area indicates that these ISO signals propagate both eastward and westward. Time-longitude diagrams of ISO signals in SSH anomalies and wind-stress curl indicate that the eastward propagating SSH anomalies is forced by wind-stress curl. This is also confirmed by lag correlation between SSH anomalies and the wind-stress-curl index (wind stress curl averaged over 109.5ºE -115ºE and 12ºN -13.5ºN). Lag correlation of SSH anomaly suggests that the westward propagating signals are free Rossby waves.