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Abstract Saline-sodic stress restricts the absorption of zinc by rice, consequently impacting the photosynthesis process of rice plants. In this experiment, Landrace 9 was selected as the test material and the potting method was employed to investigate the influence of ZnO nanoparticles (ZnO NPs) on zinc absorption and chlorophyll fluorescence in rice grown in saline-sodic land. The research findings demonstrate that the application of ZnO NPs proves to be more advantageous for the growth of rice in saline-sodic soil. Notably, the application of ZnO NPs significantly decreases the levels of Na+ and MDA in rice leaves in saline-sodic soil, while increasing the levels of K+ and Zn2+. Additionally, ZnO NPs enhances the content of chloroplast pigments, specific energy flux, quantum yield, and the performance of active PSII reaction center (PI ABS) in rice leaves under saline-sodic stress. Furthermore, the relative variable fluorescence (W K and V J) and quantum energy dissipation rate (φ Do) of rice are also reduced. Therefore, the addition of ZnO NPs enhances the transfer of electrons and energy within the rice photosystem when subjected to saline-sodic stress. This promotes photosynthesis in rice plants growing in saline-sodic land, increasing their resistance to saline-sodic stress and ultimately facilitating their growth and development.

Abstract Background The energy/protein imbalance in a low-protein diet induces lipid metabolism disorders in late-phase laying hens. Reducing energy levels in the low-protein diet to adjust the energy-to-protein ratio may improve fat deposition, but this also decreases the laying performance of hens. This study investigated the mechanism by which different energy levels in the low-protein diet influences liver lipid metabolism in late-phase laying hens through the enterohepatic axis to guide feed optimization and nutrition strategies. A total of 288 laying hens were randomly allocated to the normal-energy and normal-protein diet group (positive control: CK) or 1 of 3 groups: low-energy and low-protein diet (LL), normal-energy and low-protein diet (NL), and high-energy and low-protein diet (HL) groups. The energy-to-protein ratios of the CK, LL, NL, and HL diets were 0.67, 0.74, 0.77, and 0.80, respectively. Results Compared with the CK group, egg quality deteriorated with increasing energy intake in late-phase laying hens fed low-protein diet. Hens fed LL, NL, and HL diets had significantly higher triglyceride, total cholesterol, acetyl-CoA carboxylase, and fatty acid synthase levels, but significantly lower hepatic lipase levels compared with the CK group. Liver transcriptome sequencing revealed that genes involved in fatty acid beta-oxidation (ACOX1, HADHA, EHHADH, and ACAA1) were downregulated, whereas genes related to fatty acid synthesis (SCD, FASN, and ACACA) were upregulated in LL group compared with the CK group. Comparison of the cecal microbiome showed that in hens fed an LL diet, Lactobacillus and Desulfovibrio were enriched, whereas riboflavin metabolism was suppressed. Cecal metabolites that were most significantly affected by the LL diet included several vitamins, such as riboflavin (vitamin B2), pantethine (vitamin B5 derivative), pyridoxine (vitamin B6), and 4-pyridoxic acid. Conclusion A lipid metabolism disorder due to deficiencies of vitamin B2 and pantethine originating from the metabolism of the cecal microbiome may be the underlying reason for fat accumulation in the liver of late-phase laying hens fed an LL diet. Based on the present study, we propose that targeting vitamin B2 and pantethine (vitamin B5 derivative) might be an effective strategy for improving lipid metabolism in late-phase laying hens fed a low-protein diet.

Selective activation of Pt(IV) prodrugs within tumors has emerged as a promising strategy in tumor treatment. Although progress has been made with photo- and ultrasound-activated Pt(IV) prodrugs, concerns remain over the non-specific activation of photosensitizers (PS) and the potential for phototoxicity and chemical toxicity. In this study, a sequential dual-locked Pt(IV) nano-prodrug that can be activated by both the acidic tumor microenvironment and light was developed. The Pt(IV) prodrug was prepared by conjugating PS-locked Pt(IV) to a polymeric core, which was then chelated with metallo iron to lock its photoactivity and form a metallo-nano prodrug. Under acidic tumor microenvironment conditions, the metallo-nano prodrug undergoes dissociation of iron, triggering a reduction process in oxaliplatin under light irradiation, resulting in the activation of both chemotherapy and photodynamic therapy (PDT). Additionally, the prodrug could induce metallo-triggered ferroptosis and polarization of tumor-associated macrophages (TAM), thereby enhancing tumor inhibition. The dual-lock strategy employed in a nanoparticle delivery system represents an expansion in the application of platinum-based anticancer drugs, making it a promising new direction in cancer treatment.

Understanding how environmental factors influence the responsiveness of crop yield to growing season precipitation (GSP) can reduce the risk of yield fluctuations, ensuring stable crop production. This study involved the cultivation of rainfed potatoes at three locations within the climatic transition zone of Northwest China. We employed stepwise linear regression and machine learning techniques to pinpoint the key environmental factors influencing potato tuber yield and the yield-precipitation relationship. The slope of the water-limited yield potential relationship for potatoes was determined to be 172.1 ​kg ​ha−1 ​mm−1, with an intercept at 121.2 ​mm. The potato tuber yield exhibited an upward trend with increasing GSP but declined once the precipitation exceeded 400 ​mm. However, GSP alone explained up to 30% of the variability in potato tuber yield. Factors such as soil moisture at planting, maximum temperatures during the tuber stolon initiation and bulking stages, diurnal temperature fluctuations at maturity, and excessive precipitation events during the growing season significantly influenced potato tuber yield, and consequently, the relationship between yield and GSP. Conversely, climatic factors accounted for more than 63% of the variation in potato tuber yield, with the multiple linear regression model yielding the best results. This was especially evident when the yield-precipitation relationship was categorized into two groups based on the amount and distribution of GSP, maximum temperature, and radiation levels. This analysis suggested that preventing unnecessary water evaporation when precipitation is low, improving drainage when precipitation is high, and planting potato on an optimal date can advance potato production.

Aiming at the problem of cutting roof and retaining roadway on the right third working face of 16#, east second mining area of Jixian Coal Mine in Shuangyashan city, the mechanical model of surrounding rock of cutting roof and retaining roadway is established, and the theoretical calculation formula of key parameters such as cutting height and cutting angle is given; the influence of roof cutting parameters (roof cutting height and angle) on the stress distribution and deformation characteristics of the surrounding rock is further analyzed. The numerical simulation results show that the greater the roof cutting height is, the better the relief effect is; with the increase of the cutting angle, the side stress of solid coal shows an increasing trend, which also creates certain conditions for the caving of gangue. The similar simulation results show that when the coal seam is mined to a certain distance, the upper rock stratum in the goaf collapses at the same time, and the rock mass in the slit range and caving rock mass form a hinged joint, that is, the side filling of the roadway is basically completed, and the structure of “low cantilever beam + high hinged rock beam” is formed above the roadway. The engineering practice show that the cutting height is 8.0 m (2.39 m medium sandstone), the cutting angle is 10°, and the spacing of the drilling holes is 600 mm. When it is about 60 m away from the working face, the roof subsidence is 168 mm, the solid coal wall displacement is 212 mm, the deformation of the gangue wall is almost 0, and the bottom heave is 120 mm.

In order to improve the Hoek-Brown strength criterion not considering the influence of intermediate principal stress on rock strength, the evolutions of rock strength were investigated according to the results of the rock true triaxial compression test. The intermediate principal stress coefficient was introduced to quantify the effect on rock strength. Considering the relationships between the parameter of the Hoek-Brown strength criterion and the rock stress levels, the modified Hoek-Brown strength criterion for the true triaxial stress was then established with the Lagrange interpolation method. The spatial envelope characteristics of the modified strength criterion were analyzed. The best-fitting errors of seven rocks with true triaxial test results were finally compared with the three other three-dimensional strength criteria to verify the rationality of the modified strength criterion. The research results indicate that the rock strength increases gradually with the increase of minimum principal stress, and increases first and then decreases with the increase of intermediate principal stress, which presents a significant interval effect. The modified strength criterion not only has the advantage of the Hoek-Brown strength criterion in the nonlinear characteristics of the meridian plane but also describes the basic characteristics of rock strength in stress space. The spatial envelope surfaces for the modified strength criteria with the linear and quadratic interpolation are the non-equilateral hexagonal pyramidal surfaces and conical surfaces, which can meet the requirement of continuous smoothness in the tension-compression meridian plane interval. The modified strength criterion can better predict the true triaxial strength of rocks and reasonably reflect the influence of intermediate principal stress on the rock strength. The modified strength criterion with the quadratic interpolation improves the prediction accuracy of rock strength by about 1.2 to 2.0 times compared to the criterion with linear interpolation. The modified strength criterion has good agreement with the true triaxial test results of different hard and brittle rocks compared with other true triaxial strength criteria, which shows the applicability and reliability of the modified strength criterion.

Abstract METTL3 is the catalytic subunit of the methyltransferase complex, which mediates m6A modification to regulate gene expression. In addition, METTL3 regulates transcription in an enzymatic activity-independent manner by driving changes in high-order chromatin structure. However, how these functions of the methyltransferase complex are coordinated remains unknown. Here we show that the methyltransferase complex coordinates its enzymatic activity-dependent and independent functions to regulate cellular senescence, a state of stable cell growth arrest. Specifically, METTL3-mediated chromatin loops induce Hexokinase 2 expression through the three-dimensional chromatin organization during senescence. Elevated Hexokinase 2 expression subsequently promotes liquid-liquid phase separation, manifesting as stress granule phase separation, by driving metabolic reprogramming. This correlates with an impairment of translation of cell-cycle related mRNAs harboring polymethylated m6A sites. In summary, our results report a coordination of m6A-dependent and -independent function of the methyltransferase complex in regulating senescence through phase separation driven by metabolic reprogramming.

Abstract In recent decades boreal wildfires have occurred frequently over eastern Siberia, leading to increased emissions of carbon dioxide and pollutants. However, it is unclear what factors have contributed to recent increases in these wildfires. Here, using the data we show that background eastern Siberian Arctic warming (BAW) related to summer Russian Arctic sea-ice decline accounts for ~79% of the increase in summer vapor pressure deficit (VPD) that controls wildfires over eastern Siberia over 2004-2021 with the remaining ~21% related to internal atmospheric variability associated with changes in Siberian blocking events. We further demonstrate that Siberian blocking events are occurring at higher latitudes, are more persistent and have larger zonal scales and slower decay due to smaller meridional potential vorticity gradients caused by stronger BAW under lower sea-ice. These changes lead to more persistent, widespread and intense high-latitude warming and VPD, thus contributing to recent increases in eastern Siberian high-latitude wildfires.

Abstract The synthesis of constrained 12-membered rings is notably difficult. The main challenges result from constraints during the linear peptide cyclization. Attempts to overcome constraints through excessive activation frequently cause peptidyl epimerization, while insufficient activation of the C-terminus hampers cyclization and promotes intermolecular oligomer formation. We present a β-thiolactone framework that enables the synthesis of cyclo-tetrapeptides via direct aminolysis. This tactic utilizes a mechanism that restricts C-terminal carbonyl rotation while maintaining high reactivity, thereby enabling efficient head-to-tail amidation, reducing oligomerization, and preventing epimerization. A broad range of challenging cyclo-tetrapeptides ( > 20 examples) are synthesized in buffer and exhibits excellent tolerance toward nearly all proteinogenic amino acids. Previously unattainable macrocycles, such as cyclo-L-(Pro-Tyr-Pro-Val), have been produced and identified as μ-opioid receptor (MOR) agonists, with an EC50 value of 2.5 nM. Non-epimerizable direct aminolysis offers a practical solution for constrained peptide cyclization, and the discovery of MOR agonist activity highlights the importance of overcoming synthetic challenges for therapeutic development.

Abstract To scavenge energy from imperceptible vibrations, this paper investigates the broadband response and output performance of a microvibrational piezoelectric energy harvesting system with mechanical stopper. The energy harvesting system comprises a cantilever beam made of piezoelectric material, which is affixed with a coil at its unbound end and a mechanical stopper. The coil is placed in a magnetic field to provide an ultra‐low level excitation. The electromechanical model is derived according to force integration method (FIM) and Hertz's contact theory, and numerical simulations are undertaken to evaluate the influence of the excitation level, and the gap on the performance. For the linear counterpart without stopper, experimental results indicate the system generates a peak power of 24.12 μW with matched resistance under excitation with a level of 0.003 N and a frequency of 200.3 Hz. When a polydimethylsiloxane (PDMS) stopper is introduced, the vibration of the piezoelectric beam exhibits an obvious nonlinearity with an amplitude of micron scale. Increasing the excitation level and decreasing the gap could efficiently broaden the response bandwidth. Experimental results demonstrate that a copper stopper with larger elastic modulus results in a wider response frequency range, and the half‐power bandwidth could reach 37.1 Hz under excitation with a level of 0.003 N.

Abstract Background Emerging evidence suggests that alterations in BCAA metabolism may contribute to the pathogenesis of sarcopenia. However, the relationship between branched-chain amino acids (BCAAs) and sarcopenia is incompletely understood, and existing literature presents conflicting results. In this study, we conducted a community-based study involving > 100,000 United Kingdom adults to comprehensively explore the association between BCAAs and sarcopenia, and assess the potential role of muscle mass in mediating the relationship between BCAAs and muscle strength. Methods Multivariable linear regression analysis examined the relationship between circulating BCAAs and muscle mass/strength. Logistic regression analysis assessed the impact of circulating BCAAs and quartiles of BCAAs on sarcopenia risk. Subgroup analyses explored the variations in associations across age, and gender. Mediation analysis investigated the potential mediating effect of muscle mass on the BCAA-muscle strength relationship. Results Among 108,017 participants (mean age: 56.40 ± 8.09 years; 46.23% men), positive associations were observed between total BCAA, isoleucine, leucine, valine, and muscle mass (beta, 0.56–2.53; p

Abstract Background Esophageal cancer represents a significant public health concern; however, reliable diagnostic and prognostic markers have not been established. This study aimed to investigate the clinical value of plasma D-dimer levels in patients with esophageal cancer. Methods Overall, 120 patients with esophageal cancer who underwent radical surgical resection at our department between January 2019 and 2020 were included (esophageal cancer group). Plasma D-dimer levels were measured preoperatively and on postoperative days 1 and 14. Additionally, 60 healthy participants (control group) with measured plasma D-dimer levels were included. The preoperative D-dimer levels and positive D-dimer test rates were compared between the groups. The 3-year survival rate in patients with esophageal cancer was calculated using the Kaplan–Meier method. Results Preoperative D-dimer concentration in the esophageal cancer group was (0.65 ± 0.859 µg/mL) significantly higher than that in the control group (0.32 ± 0.369 µg/mL). The positivity rate in the esophageal cancer group (35.0%, 42/120) was significantly higher than that in the control group (15%, 9/60). D-dimer concentrations were significantly higher 1 day postoperatively than preoperatively. Conversely, D-dimer concentrations were significantly lower 14 days postoperatively than preoperatively. Patients in the esophageal cancer group with plasma D-dimer concentrations ≤ 0.5 µg/mL had significantly higher 3-year survival rates than those with higher concentrations. In the logistic multivariate analysis, tumor pathological stage and preoperative plasma D-dimer levels were independent prognostic factors of 3-year survival rates in patients with esophageal cancer. Conclusion Plasma D-dimer concentrations are clinically valuable in esophageal cancer diagnosis, postoperative recurrence monitoring, and prognosis prediction.

Abstract Economic productivity depends on reliable access to electricity, but the extreme shortage events of variable wind-solar systems may be strongly affected by climate change. Here, hourly reanalysis climatological data are leveraged to examine historical trends in defined extreme shortage events worldwide. We find uptrends in extreme shortage events regardless of their frequency, duration, and intensity since 1980. For instance, duration of extreme low-reliability events worldwide has increased by 4.1 hours (0.392 hours per year on average) between 1980–2000 and 2001–2022. However, such ascending trends are unevenly distributed worldwide, with a greater variability in low- and middle-latitude developing countries. This uptrend in extreme shortage events is driven by extremely low wind speed and solar radiation, particularly compound wind and solar drought, which however are strongly disproportionated. Only average 12.5% change in compound extremely low wind speed and solar radiation events may give rise to over 30% variability in extreme shortage events, despite a mere average 1.0% change in average wind speed and solar radiation. Our findings underline that wind-solar systems will probably suffer from weakened power security if such uptrends persist in a warmer future.

Abstract Fermenting Chinese medicinal herbs could enhance their bioactivities. We hypothesized probiotic-fermented gastrodia elata Blume (GE) with better potential to alleviate insomnia than that of unfermented, thus the changes in chemical composition and the insomnia-alleviating effects and mechanisms of fermented GE on pentylenetetrazole (PTZ)-induced insomnia zebrafish were explored via high-performance liquid chromatography (HPLC) and mass spectroscopy-coupled HPLC (HPLC-MS), phenotypic, transcriptomic, and metabolomics analysis. The results demonstrated that probiotic fermented GE performed better than unfermented GE in increasing the content of chemical composition, reducing the displacement, average speed, and number of apoptotic cells in zebrafish with insomnia. Metabolomic investigation showed that the anti-insomnia effect was related to regulating the pathways of actin cytoskeleton and neuroactive ligand-receptor interactions. Transcriptomic and reverse transcription qPCR (RT-qPCR) analysis revealed that secondary fermentation liquid (SFL) significantly modulated the expression levels of neurod1, msh2, msh3, recql4, ercc5, rad5lc, and rev3l, which are mainly involved in neuron differentiation and DNA repair. Collectively, as a functional food, fermented GE possessed potential for insomnia alleviation.

Abstract African swine fever (ASF) is a highly contagious disease of domestic and wild pigs caused by African swine fever virus (ASFV). The mortality rate associated with ASF is remarkably high, almost approaching 100%. Since the introduction of ASF into China in 2018, its rapid spread has caused marked economic losses in the country’s swine industry. To date, there are no safe and effective commercial vaccines or antiviral drugs against ASF; thus, there is an urgent need to develop novel prevention and control strategies. Traditional Chinese medicine (TCM), which comprises various herbs that are abundant in various potential functional components, holds great promise for the prevention and control of ASF. Here, we provide a comprehensive review of the advancements in TCM and the effects of its compound formulas against ASF, including the antiviral abilities, immunoregulatory activities, and practical application of these formulas for the prevention and control of ASF. We specifically examined the potential and constraints of natural product-derived extracts and TCM formulas in combating ASFV. This review aims to offer insights and ideas for the holistic management and containment of ASF.

Abstract Photoluminescence (PL) colour-changing materials in response to photostimulus play an increasingly significant role in intelligent applications for their programmability. Nevertheless, current research mainly focuses on photochemical processes, with less attention to PL transformation through uniform aggregation mode adjustment. Here we show photochromic luminescence in organic crystals (e.g. dimethyl terephthalate) with PL varying from dark blue to purple, then to bright orange-red, and finally to red. This change is attributed to the emergence of clusters with red emission, which is barely achieved in single-benzene-based structures, thanks to the subtle molecular rearrangements prompted by light. Crucial to this process are the through-space electron interactions among molecules and moderate short contacts between ester groups. The irradiated crystals exhibit reversible PL transformation upon sufficient relaxation, showing promising applications in information storage and smart optoelectronic devices. This research contributes to the development of smart photochromic luminescent materials with significant PL colour transformations through molecular rearrangement.

Abstract Alzheimer’s disease (AD) is a serious brain disorder characterized by the presence of beta-amyloid plaques, tau pathology, inflammation, neurodegeneration, and cerebrovascular dysfunction. The presence of chronic neuroinflammation, breaches in the blood-brain barrier (BBB), and increased levels of inflammatory mediators are central to the pathogenesis of AD. These factors promote the penetration of immune cells into the brain, potentially exacerbating clinical symptoms and neuronal death in AD patients. While microglia, the resident immune cells of the central nervous system (CNS), play a crucial role in AD, recent evidence suggests the infiltration of cerebral vessels and parenchyma by peripheral immune cells, including neutrophils, T lymphocytes, B lymphocytes, NK cells, and monocytes in AD. These cells participate in the regulation of immunity and inflammation, which is expected to play a huge role in future immunotherapy. Given the crucial role of peripheral immune cells in AD, this article seeks to offer a comprehensive overview of their contributions to neuroinflammation in the disease. Understanding the role of these cells in the neuroinflammatory response is vital for developing new diagnostic markers and therapeutic targets to enhance the diagnosis and treatment of AD patients.

Abstract Danshen, a prominent herb in traditional Chinese medicine (TCM), is known for its potential to enhance physiological functions such as blood circulation, immune response, and resolve blood stasis. Despite the effectiveness of COVID-19 vaccination efforts, some individuals still face severe complications post-infection, including pulmonary fibrosis, myocarditis arrhythmias and stroke. This study employs a network pharmacology and molecular docking approach to investigate the potential mechanisms underlying the therapeutic effects of candidate components and targets from Danshen in the treatment of complications in COVID-19. Candidate components and targets from Danshen were extracted from the TCMSP Database, while COVID-19-related targets were obtained from Genecards. Venn diagram analysis identified common targets. A Protein–Protein interaction (PPI) network and gene enrichment analysis elucidated potential therapeutic mechanisms. Molecular docking evaluated interactions between core targets and candidate components, followed by molecular dynamics simulations to assess stability. We identified 59 potential candidate components and 123 targets in Danshen for COVID-19 treatment. PPI analysis revealed 12 core targets, and gene enrichment analysis highlighted modulated pathways. Molecular docking showed favorable interactions, with molecular dynamics simulations indicating high stability of key complexes. Receiver operating characteristic (ROC) curves validated the docking protocol. Our study unveils candidate compounds, core targets, and molecular mechanisms of Danshen in COVID-19 treatment. These findings provide a scientific foundation for further research and potential development of therapeutic drugs.

Abstract Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) poses a major threat to the global swine industry, yet effective prevention and control measures remain elusive. This study unveils Nitazoxanide (NTZ) as a potent inhibitor of PRRSV both in vitro and in vivo. Through High-Throughput Screening techniques, 16 potential anti-PRRSV compounds are identified from a library comprising FDA-approved and pharmacopeial drugs. We show that NTZ displays strong efficacy in reducing PRRSV proliferation and transmission in a swine model, alleviating viremia and lung damage. Additionally, Tizoxanide (TIZ), the primary metabolite of NTZ, has been identified as a facilitator of NMRAL1 dimerization. This finding potentially sheds light on the underlying mechanism contributing to TIZ’s role in augmenting the sensitivity of the IFN-β pathway. These results indicate the promising potential of NTZ as a repurposed therapeutic agent for Porcine Reproductive and Respiratory Syndrome (PRRS). Additionally, they provide valuable insights into the antiviral mechanisms underlying NTZ’s effectiveness.

Abstract Background We aimed to explore the association and potential causality between polyunsaturated fatty acids concentrations and the risk of periodontal disease. Materials and methods Data were collected from the 2011–2014 National Health and Nutrition Examination Survey (NHANES). Weighted logistic regression analysis and restricted cubic spline (RCS) analysis were used to analyse the associations of the concentrations of omega-3 and omega-6 fatty acids and the omega-6/omega-3 fatty acids ratio with the risk of periodontitis. E-value and propensity score matching (PSM) analyses were used for sensitivity analyses. In addition, two-sample Mendelian randomisation (MR) analyses were performed to assess the potential causal impact of the concentrations of those fatty acids on periodontitis risk. Results A total of 2462 participants from the NHANES were included. Logistic regression analysis revealed that high omega-3 fatty acids levels were negatively associated with the risk of developing periodontitis (P 0.05). Conclusion Although omega-3 fatty acids and the omega-6/omega-3 fatty acids ratio were associated with the risk of periodontitis in cross-sectional studies, the MR results did not support a causal relationship between them. Therefore, there is no indication that an increase in the omega-3 fatty acids concentration or a decrease in the omega-6/omega-3 fatty acids ratio may be beneficial for preventing periodontitis.

IntroductionSuspended particulate matter (SPM) is a critical indicator of water quality and has a significant impact on the nearshore ecological environment. Consequently, the quantitative evaluation of SPM concentrations is essential for managing nearshore environments and planning marine resources.MethodsThis study utilized Sentinel-2’s single band and water index variables to develop a remote sensing inversion model for oceanic SPM in the estuary of the Pinglu Canal in China. Six machine learning algorithms were employed: K-nearest neighbor regression (KNNR), AdaBoost regression (ABR), random forest (RF), gradient boosting regression (GBR), extreme gradient boosting regression (XGBR), and light generalized boosted regression (LGBM). The model with the optimal performance was then selected for further analysis. This research applied the established model to investigate the spatial-temporal dynamics of SPM from 2021 to 2023.ResultsThe findings indicated that (1) the XGBR algorithm exhibited superior performance (R2 = 0.9042, RMSE = 3.0258 mg/L), with LGBM (R2 =0.8258, RMSE = 4.0813 mg/L) and GBR (R2 = 0.823, RMSE = 4.3477 mg/L) also demonstrating effective fitting. However, the ABR, RF, and KNNR algorithms produced less satisfactory fitting results. (2) Additionally, the study revealed that the combination of input variables in the XGBR algorithm was more accurate than single-variable inputs. (3) The contribution of single-band variables to the XGBR algorithm surpassed that of water index variables, with B12, B4, and B11 emerging as the top three influential variables in the model. (4) The annual SPM concentration in the study area exhibited an overall increasing trend, while its spatial distribution generally decreased from the estuary toward the Maowei Sea and Qinzhou Bay.DiscussionThe combination of Sentinel-2 data and XGBR model has shown good performance in retrieving SPM concentration, providing a new method and approach for large-scale estimation of SPM concentration.

Abstract Radiotherapy is a curative arsenal for prostate cancer (PCa), but radioresistance seriously compromises its effectiveness. Dysregulated RNA splicing factors are extensively involved in tumor progression. Nonetheless, the role of splicing factors in radioresistance remains largely unexplored in PCa. Here, 23 splicing factors that are differentially expressed between PCa and adjacent normal tissues across multiple public PCa databases are identified. Among those genes, polypyrimidine tract binding protein 1 (PTBP1) is significantly upregulated in PCa and is positively associated with advanced clinicopathological features and poor prognosis. Gain‐ and loss‐of‐function experiments demonstrate that PTBP1 markedly reinforces genomic DNA stability to desensitize PCa cells to irradiation in vitro and in vivo. Mechanistically, PTBP1 interacts with the heterogeneous nuclear ribonucleoproteins (hnRNP) associated with lethal yellow protein homolog (RALY) and regulates exon 5 splicing of DNA methyltransferase 3b (DNMT3B) from DNMT3B‐S to DNMT3B‐L. Furthermore, upregulation of DNMT3B‐L induces promoter methylation of dual‐specificity phosphatase‐2 (DUSP2) and subsequently inhibits DUSP2 expression, thereby increasing radioresistance in PCa. The findings highlight the role of splicing factors in inducing aberrant splicing events in response to radiotherapy and the potential role of PTBP1 and DNMT3B‐L in reversing radioresistance in PCa.

The coal-fired power plant (CFPP) coupled with the molten salt thermal energy storage system is a potential way to improve its flexibility and peak-shaving ability. The steam generation system (SGS) is a suitable choice to convert the feed water into hot steam by using the heat from the molten salt. In this paper, the schematic of an SGS coupled with the CFPP is presented. A dynamic model of the SGS basing lump parameter method is established and validated to investigate its dynamic response characteristics. Five different disturbance experiments including feed water inlet parameters, molten salt inlet parameters and medium pressure steam valve, are conducted and analyzed. The dynamic response curves of molten salt and steam are obtained. Moreover, the load adjustment process of SGS with three different load changing rates (3 %, 6 %, 10 % Pe/min) under the rated conditions is compared and its influence on the SGS is analyzed. The temperature changing rates in the thick-wall components of SGS are within 2 °C/min which meets the safe operating standard. Based on the above results, the three elements control strategy with excellent robustness for the SGS safety operation is proposed and demonstrated. The results show that the response time for the SGS load regulation process and the water level fluctuation have been significantly improved as the three elements control strategy is imposed. The water level can be stabilized in 200 s with tiny fluctuation when the SGS loads down 10 % thermal load with 10 % Pe/min load changing rate. These results could provide useful references for the design and control strategy making of the CFPP coupled with the molten salt thermal energy storage system.

ObjectiveTo analyze the application value of wearable adhesive Patch ECG monitors combined with transesophageal electrophysiological study (TEPS) in the diagnosis of palpitations of unknown origin.MethodsThis was a retrospective study of patients with suspected arrhythmia who were admitted to Henan Provincial People's Hospital between October 2021 and July 2023 due to recurrent paroxysmal palpitations of unknown origin, with or without accompanying symptoms such as dizziness, amaurosis, and syncope. All patients underwent TEPS. Those who did not exhibit arrhythmia during the TEPS were selected for Patch ECG monitoring, which lasted several weeks (depending on the duration of symptom capture). The results of TEPS, Patch ECG monitors, and clinical diagnoses were observed and recorded. Sensitivity, specificity, accuracy, positive predictive value, and negative predictive value (NPV) for diagnosing palpitations of unknown origins was analyzed based on clinical diagnostic outcomes for (1) TEPS alone, (2) Patch ECG monitoring in patients with negative TEPS results, and (3) the combination of both methods.ResultsA total of 569 patients were included in this study. The TEPS results exhibited that 227 of the 569 patients did not detect arrhythmias and 342 detected arrhythmias. Of the 569 patients, 102 refused to undergo Patch ECG monitors, and 467 patients completed the entire study process. Among them, 379 cases (66.61%) were clinically diagnosed as arrhythmias. TEPS shows good performance in most evaluation indices except NPV (69.60%, 95% CI, 61.54%–77.66%). The combined diagnosis was strongly consistent with clinical diagnosis. The accuracy, sensitivity, and NPV of TEPS combined with Patch ECG monitors in the diagnosis of palpitations of unknown origin were significantly higher than those of TEPS alone.ConclusionWearable adhesive patch ECG monitors combined with TEPS can enhance the diagnostic efficiency of palpitations of unknown origin.

Objective To investigate the relationship between sleep duration and myopia among primary school students in minority regions of Southwest China.Methods The school-based, cross-sectional study was conducted from October 2020 to January 2021. All participants underwent a comprehensive ocular examination and completed a questionnaire on demographic characteristics, ophthalmological history and major environmental factors for myopia. Spherical equivalent (SE) and ocular biometric parameters were measured after cycloplegia, with myopia being defined as SE ≤−0.5 D (Diopter). Multivariate regression models were used to examine the association of sleep duration with myopia, SE and axial length (AL).Results A total of 857 students from grades 2 to 4 were included in the analysis, of which 63.6% were myopic and 62.0% belonged to ethnic minorities. Boys had a slightly higher prevalence of myopia compared with girls (66.7% vs 60.6%, p=0.06). Myopic students had longer AL, deeper anterior chamber depth and thinner central corneal thickness compared with non-myopic students (all p

IntroductionThe aim of this study was to investigate effect of energy levels on liver oxidative state and gut microbiota of laying hens fed a low-protein diet.MethodsA total of 216 laying hens (57-week-old with similar body weight) were randomly divided into 3 groups: low-energy and low-protein diet (LL: 10.73 MJ/kg), middle-energy and low-protein diet (ML: 11.15 MJ/kg), and high-energy and low-protein diet (HL: 11.57 MJ/kg) groups. The experiment lasted for 10 weeks.ResultsResults showed that the liver MDA level was higher, while the liver T-AOC level was lower in the HL group compared with LL and ML groups. The dominant phyla in LL group, ML group, and HL group were Bacteroidetes (38.08%, 39.69%, and 40.93%) and Firmicutes (16.78%, 18.37%, and 17.44%). At the genus level, Alistipes (9.45%) was abundant in the LL group. Bacteroides (14.46%), Phocaeicola (4.48%) and Precotella (2.95%) were highest in the ML group. Parabacteroides (1.78%) and Desulfovibrio (1.02%) were highest in the HL group. At the species level, Bacteroides fragilis (0.42%) is lowest in LL group, Desulfovibrio piger increased with the increase of energy. MDA was significantly and positively correlated with Methanobrevibacter woesei between ML group and HL groups (p < 0.05). T-AOC was highly significantly and positively correlated with Phocaeicola sp. Sa1YUN3 (p < 0.01) .DiscussionThis study found that high-energy and low-protein diets might cause liver oxidative stress by gut microbes in the laying hens.

Seashore paspalum (Paspalum vaginatum) is a warm-season and perennial turfgrass and is known for its cadmium (Cd)-stress tolerance. Here, a Phosphoproteomics analysis was performed to examine the key proteins relating to Cd tolerance in seashore paspalum. Fructose 1,6-biphosphate aldolase, PvFBA1, was identified for its phosphorylated state after exposure to Cd stress. Specifically, the phosphorylation of PvFBA1 was enhanced in several metabolic pathways, including pentose phosphate pathway (PPP), carbon fixation and biosynthesis of amino acids under Cd stress. By transforming PvFBA1 into Arabidopsis, the PvFBA1-OE plants exhibited longer roots, greater FBA activity and higher soluble sugar content than WT under 100 µM CdCl2 treatment. By expressing the PvFBA1 in yeast, a serine 50 phosphorylation site was identified as functional site. By microscale thermophoresis experiment, we indicted that PvFBA1can bind Cd directly enhancing its phosphorylation level to alleviate the damage of Cd. This finding may provide new insights into the molecular mechanisms of plants Cd tolerance.

Research on ecosystem carbon sinks is vital for developing policies to reduce emissions and enhance carbon sequestration. Analyzing the temporal and spatial trends of carbon sinks and their driving mechanisms is crucial for guiding policy and measures. Traditional methods for estimating carbon sinks in karst regions focus primarily on vegetation-soil carbon sinks, mainly comprising net primary productivity (NPP) and soil heterotrophic respiration (Rh). However, these methods overlook the unique karst carbon sink (KCS), leading to significant uncertainties in carbon sink evaluations. This study focuses on the karst peak cluster depression basin in southwest Guangxi and proposes a Karst Ecosystem Carbon Sink Assessment System (KECAS) based on the “water-rock-soil-air-biology” coupling framework, considering lithological characteristics. Theil-Sen trend analysis and restricted cubic spline (RCS) regression were used to explore the temporal and spatial variations and driving mechanisms of carbon sinks in the area from 2000 to 2022. The results show the following: (1) The total carbon sink flux (TCSF) in the study area has shown an upward trend over time, with a 23-year average ranging from 538 to 929 g CO2 m−2 a−1 and a growth rate of 3.1039a−1. This means TCSF increases by 6.78 g CO2 m−2 a−1 each year. (2) Spatially, areas with increasing carbon sinks cover 34.17 % of the study area, mainly in Chongzuo, Nanning, and Wenshan Prefecture. Areas with decreasing carbon sinks cover 11.79 % of the total area, mainly in Chongzuo, with smaller areas in Nanning and Baise. The mean TCSF distribution is higher in the northwest and lower in the southeast. (3) The critical thresholds for influencing factors were identified using RCS regression: precipitation (1430 mm), temperature (17.9 °C), evapotranspiration (855 mm), NDVI (0.63), DEM (637 m), slope (16.3°), nighttime light index (6.21), and population density (421 people/km2). (4) Areas with high carbon sink supply potential, based on natural and human thresholds, account for 25.09 % and 92.97 % of the southwestern Guangxi study area, respectively. When considering both natural and human conditions, high potential areas account for approximately 22.31 % of the total study area. These findings provide a scientific basis for carbon sink protection and management and offer valuable references for government decision-making.

Background: This study investigates the association between dietary flavonoid intake and the incidence of mild cognitive impairment (MCI) through a matched case-control design. Methods: Dietary intake was assessed using a food frequency questionnaire, comparing the intake of flavonoids between individuals with MCI and those with normal cognitive function. Logistic regression analysis was employed to evaluate the correlation between dietary flavonoid intake and the risk of MCI. Additionally, blood concentrations of S100β, a marker of the blood-brain barrier (BBB) integrity, were measured using electrochemiluminescence immunoassay, and Pearson correlation analysis was conducted to explore the relationship between dietary flavonoid intake and blood S100β levels. Results: Compared to participants with normal cognition, those with MCI had significantly lower dietary intakes of total flavonoids, isoflavones, daidzein, glycitein, genistein, kaempferol, myricetin, flavonols, and anthocyanidins, while the intake of peonidin was significantly higher. Univariate logistic regression analysis indicated that high dietary intake of total flavonoids, isoflavones, daidzein, glycitein, genistein, kaempferol, myricetin, and flavonols was negatively correlated with MCI, whereas peonidin intake was positively correlated with MCI. Multivariate logistic regression analysis confirmed these findings. Pearson correlation analysis revealed a significant negative correlation between dietary intake of kaempferol and myricetin and blood S100β levels. Conclusion: Increasing the dietary intake of total flavonoids, isoflavones, daidzein, glycitein, genistein, and flavonols appears to be a protective factor against MCI, while higher intake of peonidin is associated with an increased risk of MCI. The protective or adverse effects of these flavonoids may not be related to the permeability of the BBB. Myricetin and kaempferol intake may protect cognitive function by maintaining BBB integrity.

Abstract Adult T cell leukaemia (ATL), caused by infection with human T‐ lymphotropic virus type 1 (HTLV‐1), is often complicated by hypercalcemia and osteolytic lesions. Therefore, we studied the communication between patient‐derived ATL cells (ATL‐PDX) and HTLV‐1 immortalized CD4+ T cell lines (HTLV/T) with osteoclasts and their effects on bone mass in mice. Intratibial inoculation of some HTLV/T leads to a profound local decrease in bone mass similar to marrow‐replacing ATL‐PDX, despite the fact that few HTLV/T cells persisted in the bone. To study the direct effect of HTLV/T and ATL‐PDX on osteoclasts, supernatants were added to murine and human osteoclast precursors. ATL‐PDX supernatants from hypercalcemic patients promoted the formation of mature osteoclasts, while those from HTLV/T were variably stimulatory, but had largely consistent effects between human and murine cultures. Interestingly, this osteoclastic activity did not correlate with expression of osteoclastogenic cytokine receptor activator of nuclear factor kappa‐B ligand (RANKL), suggesting an alternative mechanism. HTLV/T and ATL‐PDX produce small extracellular vesicles (sEV), known to facilitate HTLV‐1 infection. We hypothesized that these sEV also mediate bone loss by targeting osteoclasts. We isolated sEV from both HTLV/T and ATL‐PDX, and found they carried most of the activity found in supernatants. In contrast, sEV from uninfected activated T cells had little effect. Analysis of sEV (both active and inactive) by mass spectrometry and electron microscopy confirmed absence of RANKL and intact virus. Viral proteins Tax and Env were only present in sEV from the active, osteoclast‐stimulatory group, along with increased representation of proteins involved in osteoclastogenesis and bone resorption. sEV from osteoclast‐active HTLV/T injected over mouse calvaria in the presence of low‐dose RANKL caused more osteolysis than osteoclast‐inactive sEV or RANKL alone. Thus, HTLV‐1 infection of T cells can cause release of sEV with strong osteolytic potential, providing a mechanism beyond RANKL production that modifies the bone microenvironment, even in the absence of overt leukaemia.

BackgroundOxidative stress plays a significant role in aging and cancer, yet there is currently a lack of research utilizing machine learning models to examine the relationship between oxidative stress and prognosis in elderly non-small cell lung cancer (NSCLC) patients.MethodsThis study included elderly NSCLC patients who underwent radical lung cancer resection from January 2012 to April 2018, exploring the relationship between Oxidative Stress Score (OSS) and prognosis. Machine learning techniques, including Decision Trees (DT), Random Forest (RF), and Support Vector Machine (SVM), were employed to develop predictive models for 5-year overall survival (OS).ResultsThe datasets consisted of 1647 patients in the training set, 705 in the internal validation set, and 516 in the external validation set. An OSS was formulated from six systemic oxidative stress biomarkers, such as albumin, total bilirubin, and blood urea nitrogen, among others. Boruta variable importance analysis identified low OSS as a key indicator of poor prognosis. The OSS was subsequently integrated into the DT, RF, and SVM models for training. These models, optimized through hyperparameter tuning on the training set, were then evaluated on the internal and external validation sets. The RF model demonstrated the highest predictive performance, with an Area Under the Receiver Operating Characteristic Curve (AUC) of 0.794 in the internal validation set, compared to AUCs of 0.711 and 0.760 for the DT and SVM models, respectively. Similarly, in the external validation set, the RF model achieved an AUC of 0.784, outperforming the DT and SVM models, which had AUCs of 0.699 and 0.730, respectively. Calibration plots confirmed the RF model’s superior calibration, followed by the SVM model, with the DT model performing the poorest.ConclusionThe OSS-based clinical prediction model, constructed using machine learning methodologies, effectively predicts the prognosis of elderly NSCLC patients post-radical surgery.

ObjectiveThis study aims to evaluate the effects of pleural reconstruction during rib compartment tumor resection surgery on postoperative outcomes, including drainage volume, drainage duration, hospital stay, complications, and pain control.MethodsA retrospective analysis was conducted on 142 patients who underwent rib compartment tumor resection surgery at Beijing Jishuitan Hospital from January 2013 to October 2023. The patients were divided into two groups: those who received pleural reconstruction and those who did not. Data were collected from hospital medical records and outpatient care records, focusing on postoperative drainage volume, total drainage time, length of hospital stay, complications, and pain scores. Continuous variables were compared using t-tests or nonparametric tests, while categorical variables were analyzed using chi-square tests or Fisher's exact tests.ResultsThe analysis showed no significant differences between the two groups in terms of postoperative complications and pain thresholds. However, patients who underwent pleural reconstruction had significantly lower postoperative drainage volume (937.74 ± 855.97 vs. 1,595.26 ± 1,054.50 ml, p

Alfalfa (Medicago sativa L.) grassland is prone to degradation following multi-year maintenance. Yet, its mechanism regarding the stoichiometry of carbon (C) and nitrogen (N) across plant–soil system is still unclear. To address this issue, the method of space-for-time sampling was employed to investigate alfalfa grasslands with five planting years (5-, 8-, 10-, 15-, and 20-year periods) in the semiarid Loess Plateau. The results showed that the alfalfa above- and underground biomass decreased steadily decrease after the fifth to eighth years, showing a degradation tendency with the extension of planting duration. The mean weight diameter of aggregate registered an increase with planting years. However, the C and N stocks decreased with planting years in five soil aggregate fractions. Specifically, they were the highest in the fifth year and then started to gradually decrease along the 8th, 10th, 15th, and 20th year. Redundancy and correlation analysis confirmed that the C and N stocks of soil aggregates were closely positively associated with those of plant. Overall, the highest stability of soil physical structure was found during the period from the fifth to eighth year, and, afterward, the stability declined. In conclusion, alfalfa plantation improved soil structure stability but aggravated soil C and N stocks, and biomass and soil aggregate indicators accounted for alfalfa field degradation after a certain year of plantation.

Mixed gonadal dysgenesis is caused by a variety of chromosome abnormalities, most commonly Y chromosome mosaicism. An 8-year-old boy presented with short stature for possible treatment with recombinant growth hormone. He had a history of mixed gonadal dysgenesis (hypospadias, bilateral cryptorchidism, processus vaginalis, and dysplastic immature uterus) and a series of corrective surgeries. At 14 months of age, chromosomal karyotyping revealed 46,X,+mar. Upon presentation, lab testing was consistent with the male phenotype at prepuberty. Fluorescence in situ hybridization revealed 45,X[2]/46,X,der(Y).ish psu idic(Y)(q11.2)(SRY++,DYZ3++)[38] karyotype. A literature review identified eight case reports of mixed gonadal dysgenesis associated with 45,X/46,X,idic(Y)(q11.2). Neither sex phenotype nor short stature correlated with the 46,X,idic(Y)(q11.2) mosaic ratio.

Abstract Black carbon (BC) is a highly persistent yet poorly understood component of forest soil carbon reservoirs, while its inventory, distribution, and determining factors in forest soils on a large geographic scale remain unclear. Here, we characterized soil BC across 68 Chinese forest sites using benzene polycarboxylic acid method and developed machine learning (ML) models to predict and interpret potential impacts of soil organic matter (SOM) properties, soil physiochemical properties, meteorological conditions, wildfire history, and microbial diversity on BC. Results revealed that SOM properties were the most critical in predicting BC, complemented by the negative impact of mean annual temperature and alkaline mineral composition. The superior prediction accuracy for BC with higher condensed aromaticity (more benzene hexa‐ and penta‐carboxylic acid monomers) likely results from its simpler sources and greater resistance to transformation. This study introduces an effective ML model for predicting and interpreting soil BC inventory to better understand BC cycling.

Study region: the Yellow River Basin(YRB) in China. Study focus: Ecological conservation and high-quality development of YRB has been declared as the national strategy of China. However, frequent flood hazards are a major challenge to this strategy. Further, flood behavior and impacts of the YRB are inadequately understood. We identified five floodplains using VIC-Cama model and evaluated flood risk during 1978–2015 for 50–1000-year return periods(RP) events in terms of the exposure of population (Ep), cropland (Ec), forest and grass (Efg), and settlements (Es), additionally, the contribution of climate and human behavior to flood magnitude and flood risk have been explored. New hydrological insights for the region: Results show increased Ep and Es but decreased Ec and Efg from 1978 to 2015. Exposure caused by 50–1000-year RP flood events were usually 1–2.47 times larger than those of 1978–2015. It was found that consecutive 144-day total precipitation was the prime driver of flood magnitude variation. Areas with highly-concentrated socioeconomic activities were increasingly susceptible to floods, and flood risks therefore increased during the 1978–2015 period across the YRB. Furthermore, changes in flood magnitude greatly influenced the susceptibility of population and settlements to flood risk, while spatial patterns of flood disaster-bearing bodies impacted the exposure of cropland and forest to flood risk. This study highlights YRB flood risks and relevant drivers, and helps understand flood risks of large rivers over the globe.

The coronavirus disease (COVID-19) SARS-CoV-2 virus epidemic continues to exhibit a sporadic onset trend due to the continuous variation of the novel coronavirus. However, the psychological impact of the pandemic persists. It is crucial to reflect on our experiences to better prepare for future large-scale infectious diseases. During outbreaks of infectious diseases, patients may still require orthopaedic surgery. It is crucial to prioritize the safety of medical staff and establish procedures to ensure their protection. However, with the implementation of a series of standardized operational protection procedures, orthopaedic surgeons can safely perform their duties without the risk of contracting COVID-19. There is no doubt that the orthopaedic occupational exposure protection process and perioperative management plan for global infectious diseases, such as COVID-19, require a standardized summarization process and a narrative review.

Abstract Background The optimal duration of preoperative imatinib (IM) remains controversial. This study aimed to evaluate the safety, therapeutic effectiveness, and optimal duration of preoperative IM in patients with locally advanced gastric gastrointestinal stromal tumors (GIST). Methods The clinicopathologic data of 41 patients with locally advanced gastric GIST who received preoperative IM and underwent surgical resection from January 2014 and December 2021 were retrospectively analyzed. Results After a median of 7.0 (IQR: 4.5–10) months of preoperative IM treatment, 30 patients experienced adverse events (AEs), 80% of which were grade 1/2 AEs. The mean tumor size decreased from 12.71 ± 5.34 cm to 8.26 ± 4.00 cm, with a reduction rate of 35%. Setting 8 months as the cut‐off value according to the results of ROC analysis. The proportion of laparoscopic surgery was higher in patients with short‐term (≤8 months) versus long‐term (>8 months) preoperative IM. Compared with the subtotal/total gastrectomy group, patients in the local gastrectomy group exhibited less intraoperative blood loss, shorter length of postoperative hospital stay, and fewer postoperative complications. The 3‐year recurrence‐free survival (RFS) and overall survival (OS) rates were 82.9% and 97.6%, and the expected 5‐year RFS and OS rates were 75.6% and 90.2% respectively. RFS was better in the short‐term than in the long‐term preoperative IM treatment group, and it was also better in pre‐ plus postoperative IM treatment group than that in the preoperative IM alone group. Both univariate and multivariate COX analysis showed that a higher mitotic index and long‐term preoperative IM treatment were associated with worse RFS, while postoperative IM treatment could significantly improve RFS. Conclusions The study suggests that in patients with locally advanced gastric GIST, preoperative short‐term (≤8 months) use of IM is associated with higher RFS than long‐term use.

Abstract The buried interface in the perovskite solar cell (PSC) has been regarded as a breakthrough to boost the power conversion efficiency and stability. However, a comprehensive manipulation of the buried interface in terms of the transport layer, buried interlayer, and perovskite layer has been largely overlooked. Herein, we propose the use of a volatile heterocyclic compound called 2‐thiopheneacetic acid (TPA) as a pre‐buried additive in the buried interface to achieve cross‐layer all‐interface defect passivation through an in situ bottom‐up infiltration diffusion strategy. TPA not only suppresses the serious interfacial nonradiative recombination losses by precisely healing the interfacial and underlying defects but also effectively enhances the quality of perovskite film and releases the residual strain of perovskite film. Owing to this versatility, TPA‐tailored CsPbBr3 PSCs deliver a record efficiency of 11.23% with enhanced long‐term stability. This breakthrough in manipulating the buried interface using TPA opens new avenues for further improving the performance and reliability of PSC.

Abstract In this study, the evolutional features and underlying driving mechanisms of the postmidnight‐to‐dawn equatorial plasma bubble (EPB) irregularities during the weak geomagnetic activity period on 13 November 2015 were investigated based on the multiple satellite and ground‐based observations. By using the coherent scatter radar operating at very high frequency at Fuke (19.5°N, 109.1°E, dip latitude 14.4°N), China, it was found that the freshly developed field‐aligned irregularities (FAIs) occurred within the radar's field of view around ∼04:37 LT and sustained for more than 40 min. The remarkable EPB‐related density and total electron content (TEC) depletions measured by the satellite and GNSS receivers were also observed, which indicates the persistence of EPB irregularities until ∼06 LT. The significant elevation of bottomside F‐layer's virtual height obtained by the Digisonde at Fuke as well as the upward vertical F‐layer plasma drifts derived from a nearby Digisonde at Sanya (18.4°N, 109.6°E, dip latitude 13.1°N) both imply the existence of strong eastward perturbation electric fields after local midnight. These findings suggest that the collective effects of eastward overshielding penetration electric field (PEF) resulted from the substorm onset and rapid northward turning of the interplanetary magnetic field (IMF) Bz, surpassed the role of westward undershielding prompt penetration electric field (PPEF) induced by the southward turning of IMF Bz. Thus, the former predominated in modulating the equatorial/low‐latitude zonal electric fields and raised the F‐layer considerably, which consequently boosted the growth of R‐T instability and created the favorable conditions for the postmidnight‐to‐dawn EPBs development.

Plant growth, crop yield, and pest and disease control are enhanced by PGPR (Plant growth promoting rhizobacteria), which are beneficial microorganisms found in a close symbiosis with plant roots. Phytohormones are secreted, nutrient uptake is improved, and soil properties along with the microbiological environment are regulated by these microorganisms, making them a significant focus in agricultural research. In this study, the efficient PGPR strain T1 was isolated and screened from tobacco inter-root soil, and identified and confirmed by ITS sequencing technology. Tobacco growth indicators and soil property changes were observed and recorded through potting experiments. The activities of key enzymes (e.g., sucrase, catalase, urease) in soil were further determined. High-throughput sequencing technology was utilized to sequence the soil microbial community, and combined with macro-genomics analysis, the effects of T1 strain on soil microbial diversity and metabolic pathways were explored. Following the application of T1, significant improvements were observed in the height, leaf length, and width of tobacco plants. Furthermore, the physical and chemical properties of the soil were notably enhanced, including a 26.26% increase in phosphorus availability. Additionally, the activities of key soil enzymes such as sucrase, catalase, and urease were significantly increased, indicating improved soil health and fertility. Comprehensive joint microbiomics and macrogenomics analyses revealed a substantial rise in the populations of beneficial soil microorganisms and an enhancement in metabolic pathways, including amino acid metabolism, synthesis, and production of secondary metabolites. These increase in beneficial microorganisms and the enhancement of their metabolic functions are crucial for plant growth and soil fertility. This study provides valuable references for the development of innovative microbial fertilizers and offers programs for the sustainable development of modern agriculture.

IntroductionMutations in the protein WD repeat structural domain 26 (WDR26, MIM 617424) have been identified as the cause of autosomal dominant Skraban-Deardorff syndrome, a rare genetic disorder characterized by intellectual disability (ID), developmental delay (DD), hypotonia, epilepsy, infant feeding difficulties, gait abnormalities and distinctive facial features. The objective of this study is to investigate the genetic factors that may contribute to the development of Skraban-Deardorff syndrome in affected individuals.MethodsIn this study, we used whole-exome sequencing (WES) to analyze pathogenic and likely pathogenic variants in two unrelated Chinese patients with DD and ID. We confirmed the origin of the variants by conducting Sanger sequencing and classified them according to ACMG/AMP guidelines.ResultsHere, two novel de novo variants (c.1797delC(p.His599fs*11) and c.1414C>T(p.Gln472*)) in the WDR26 gene have been identified in two Chinese patients with Skraban-Deardorff syndrome. These patients exhibit a range of symptoms, including varying degrees of ID, DD, speech delay, an abnormal wide-foot and/or stiff-legged gait, facial dysmorphism, behavioural abnormalities, with or without seizures.ConclusionsIn this study, We report two unrelated Chinese patients with Skraban-Deardorff syndrome caused by novel de novo pathogenic variants of the WDR26 gene. These patients showed a clinical phenotype similar to that of patients with the WDR26 variant. Compared to reported cases with WDR26 pathogenic variants, patient 2 presented a novel complication of severe behavioural problems, including hyperactivity, social anxiety, self-mutilation, impulsivity and violent behaviour. This research broadens the range of genetic and clinical features of Skraban-Deardorff syndrome. In addition, the symptoms may become more pronounced as the patient ages. Furthermore, our report highlights the clinical diversity of Skraban-Deardorff syndrome. The findings may assist healthcare professionals in providing more accurate genetic testing and counselling to affected families and improving the overall management of the condition.

Beijing, with the highest number of motor vehicles in China, significantly contributes to O3 pollution through substantial NOx and VOC emissions in the on-road transportation sector. Understanding the unique impact of emissions from different vehicle types on O3 levels is crucial for developing targeted strategies for O3 pollution. This study applied the Community Multiscale Air Quality Modeling System (CMAQ) to comprehensively investigate the impacts of emissions from different vehicle types on O3 levels in various regions of Beijing and to provide valuable insights into source contributions and formation processes. The results revealed that various vehicle types exhibited different spatial-temporal emission patterns, with medium-heavy duty trucks (HDT) and mini-light passenger vehicles (LDPV) identified as the primary contributors to NOx (36.1 %) and VOC (57.6 %) emissions. Using the Integrated Source Apportionment Method (ISAM) coupled in CMAQ, we found the total vehicle emissions contributed to over 20 % of daily maximum 8–h average O3 (MDA8 O3) concentration, ranked as the second largest contributor after regional transport. Contributions to O3 formation from LDPV and medium-large passenger vehicles (MDPV) were 2.6–4.0 and 4.2–6.8 ppb and mainly concentrated in urban areas, while the contributions from mini-light duty trucks (LDT) and HDT were 3.5–4.8 and 3.7–6.2 ppb and mainly concentrated in suburban areas. Through scenario analysis that removed emissions from specific types of vehicles, we found removing LDPV emissions led to decreases in daytime O3 concentration by 0.3–3.8 ppb. In contrast, removing MDPV emissions led to notable O3 increases by 4.0–11.8 ppb at rush hours. Removing LDT and HDT emissions resulted in 0.6–8.0 ppb increases in nocturnal O3 concentrations while 0.8–2.0 ppb decreases during the afternoon. This research highlights the necessity of tailoring control strategies for different vehicle types to effectively reduce O3 levels in Beijing and provides useful information for decision-makers to formulate effective measures of vehicle management in the future.

IntroductionThe present study explores the effect of ginseng stem leaf (GSL) extract on the production performance, meat quality, antioxidant status, immune function, and lipid metabolism of white feathered broilers.MethodsThere were 6 replicates in each group, with 10 broilers in each replicate. In the 42 day trial, 300 AA broilers were randomly divided into five groups: control group (CON), 1.25% GSL extract group (GSL-L), 2.5% GSL group (GSL-M), 5% GSL group (GSL-H), and 45 mg/kg chlortetracycline group (CTC).ResultsThe results showed that different doses of GSL extract could improve the body weight, feed to gain ratio (F/G), average daily feed intake (ADFI), average daily gain (ADG), and meat quality of broilers. Compared with the control group, the addition of different doses of GSL improved the antioxidant and immune abilities of broilers to varying degrees, and the effect of GSL extract was significant in the GSL-H group (p

BackgroundKindler Syndrome (KS, OMIM #173650), a rare autosomal recessive genetic disorder, is characterized by a spectrum of symptoms such as cutaneous fragility, blistering, photosensitivity, and mucosal involvement. These symptoms result from variations in the FERMT1 gene (Fermitin family member 1, OMIM: 607900), encoding kindlin-1, an essential component of focal adhesions.ObjectiveThis study aims to ascertain the potential pathogenicity of a FERMT1 variant identified in a Chinese patient and to explore the phenotypic and molecular genetic characteristics of all reported cases of Kindler Syndrome in the Chinese population.MethodsWhole-exome sequencing (WES) was performed on the patient to identify candidate variants associated with KS, and Sanger sequencing was utilized to authenticate their presence and origin. To further assess the potential impact of these genetic variants, we employed a variety of in silico prediction tools. Concurrently, a review of various databases was undertaken to ascertain and consolidate information regarding cases of KS in Chinese families.ResultsWe identified a novel likely pathogenic frameshift variant in the FERMT1 gene, specifically c.567_579delTATATATGACCCC (p.Ile190Serfs*10). The clinical presentation of this patient aligns with the diagnostic criteria for KS. The literature review reveals that the core clinical features of KS reported in the Chinese population include skin abnormalities (100%), as well as hyperkeratosis of the palms and soles (91.70%). Other clinical phenotypes encompass nail abnormalities (77.78%), abnormalities of the fingers/toes (75.00%), oral damage (70.00%), eye abnormalities (57.14%), and constipation (50.00%).ConclusionOur study enriches the genetic landscape of KS in the Chinese population and augments the understanding of phenotypic variability resulting from FERMT1 gene variants. The findings hold considerable significance for refining variant-based screening, genetic diagnosis, and comprehending the molecular pathogenesis underlying FERMT1-related disorders.

Background High cognitive reserve (CR) has been related to lower dementia risk, but its association with heart disease (HD) is unknown. We aimed to explore the relation of CR to HD and cardiac structure and function. Methods and Results Within the UK Biobank, 349 907 HD‐free participants were followed up. A composite CR indicator involving education/occupation attainment/television viewing time/confiding frequency/social connection frequency/variety of leisure activities was generated, and further categorized into low/moderate/high levels. Incident HD, including coronary HD, cardiac arrhythmia, and heart failure, was ascertained on the basis of medical records. During the follow‐up, a subsample (n=31 182) underwent cardiac magnetic resonance imaging to assess ventricular structure and function. Data were analyzed using Cox regression, Laplace regression, and linear regression. Compared with low CR, the hazard ratio and 95% CI of any HD for high CR was 0.78 (0.75–0.80) (including 0.68 [0.66–0.71] for coronary HD, 0.91 [0.87–0.95] for cardiac arrhythmia, and 0.63 [0.58–0.68] for heart failure). Furthermore, high CR was associated with delayed HD onset by 1.59 (95% CI, 1.37–1.82) years compared with low CR. In cardiac magnetic resonance imaging data analysis, compared with low CR, high CR was associated with larger left ventricular end‐diastolic volume (β, 0.13 [95% CI, 0.09–0.17]), left ventricular end‐systolic volume (β, 0.05 [95% CI, 0.01–0.10]), left ventricular stroke volume (β, 0.16 [95% CI, 0.12–0.21]), and left ventricular ejection fraction (β, 0.08 [95% CI, 0.03–0.13]). Conclusions High CR is associated with favorable HD health. Our findings suggest that the beneficial effect of CR is not limited to dementia but also HD.

Submarine fan reservoirs are important accumulation zones for oil, gas, and natural gas hydrates, offering significant potential for hydrocarbon exploration. During the deposition period of the Sanya Formation in the southern part of the Changchang Sag of the Qiongdongnan Basin, a large submarine fan developed. However, the internal structure, source-sink system, and formation mechanism of this fan remain poorly understood, posing significant challenges to exploration in this area. This paper examines the source-to-sink sedimentary processes and deposition of submarine fans, using the Changchang Sag, in the Qiongdongnan Basin in the Northern South China Sea, as an example, which will provide valuable general guidance for deep water oil and gas exploration. Based on the theories of seismic stratigraphy and seismic sedimentology, this paper utilizes techniques such as seismic facies analysis, seismic attribute optimization, paleogeomorphology reconstruction, and source-to-sink sedimentary system analysis to analyze the 3D seismic data of the study area. Research indicates that the Sanya Formation in the Changchang Sag of the Qiongdongnan Basin comprises three depositional units: submarine fan, feeder channel, and Semi-deep marine to deep marine mudstone. The submarine fan is a fan formed by the coupling and convergence of submarine fans sourced from the southwest and southeast. Internally, it is divided into three sub-facies: the proximal fan of the sand-rich submarine fan, the main body of the sand-rich submarine fan lobes, and the distal lobes of the sand-rich submarine fan. The submarine fan sourced from the southwest extends nearly north-south and is primarily fed by sediment transported through three large, banded ancient valleys. The sedimentary filling is characterized by three-phase progradation. The submarine fan sourced from the southeast extends nearly east-west and is primarily fed by sediment transported through a single large, banded ancient valley. The sedimentary filling is characterized by two-phase lateral accumulation. During the deposition period of the Sanya Formation, certain areas of the southern uplift belt were exposed for extended periods and subjected to weathering and erosion. Sediments are transported to large ancient valleys through small supply channels. A large number of sediments were transported to the southern slope of the Changchang sag through the provenance channel system such as large ancient valleys and slope belts and deposited in the center of the sag. These make up a complete system of large ancient uplifts and submarine fan source-to-sink sedimentary systems.The sedimentary model is a lobed submarine fan controlled by semi-restricted ancient valleys and expansive basins.

The peak-cluster depression is one of China’s most ecologically fragile areas, with extensive karst development. However, existing models for assessing karst ecosystem services often fail to consider the unique geological context of karst peak-cluster depressions, making it challenging to apply general international models to this area. To address these challenges, this study focused on the karst basin of Southwest China and evaluated ecosystem service in 2000 and 2020 by revising carbon fixation and soil erosion models. Using interpretable machine learning model (XGBoost-SHAP, eXtreme Gradient Boosting and SHapley Additive exPlanations), we quantified the nonlinear characteristics and threshold effects of ecosystem service trade-offs and synergies. Our findings include the following: (1) Carbon fixation increased from 753.99 tCO2∙km−2∙a-1 in 2000 to 756.70 tCO2∙km−2∙a-1 in 2020; however, soil erosion decreased from 16.56 t∙hm−2∙a-1 to 15.12 t∙hm−2∙a-1. (2) At the basin scale, carbon fixation and soil erosion exhibited both trade-offs and synergistic relationships, with 63.3 % of the area showing a trade-off and 36.7 % showing a synergistic relationship. Trade-off relationships were prevalent in the upper and lower reaches, while the middle reaches demonstrated synergistic relationships. (3) Normalized Difference Vegetation Index (NDVI) emerged as the primary driver of changes in ecosystem service trade-offs, with NDVI, precipitation, temperature, evapotranspiration, elevation, and lithology as the most significant explanatory factors. These factors impact ecosystem service trade-offs in a nonlinear manner and exhibit pronounced threshold effects. (4) Climate factors contributed 31.65 % to ecosystem service trade-offs, geomorphic factors contributed 14.81 %, soil factors contributed 5.72 %, and human activities contributed 5.39 %. (5) Local interpretability SHAP values indicated substantial differences in the contributions of drivers at different scales to ecosystem service trade-offs. The methodology implemented in this study offers a practical approach for the sustainable and differentiated management of karst ecosystem services by integrating karst ecosystem service assessment models with interpretable machine learning methods.

To investigate the influence of wind, waves and tides on the floating state, stability and strength of stranded ships, based on the previous literature, this paper summarizes three kinds of stranded modes of stranded ships and establishes a mechanical model of the action of wind, waves and tides on stranded ships. The stability, ground force, wind moment, shear force, bending moment, and torque were obtained by simulating the floating state change course of the stranded ship in different wind, wave, and tidal environments. The mechanical analysis and calculation of the stranded ship under different working cases show that wind and waves will cause the ship to capsize under the 4-level sea condition. Under 3-level sea conditions, the balanced arrangement of multiple stranded points limits the floating state of the ship and ensures its stability. In a wave period, the maximum stability of each working case exceeded the stability of the other environments in each working case by at least 0.156 m, 0.169 m, 0.044 m and 0.502 m, respectively. When the sea condition is level 4, only under the action of waves is the displacement of the stranded ship less than that of the other working cases, and the total amount of ground force is greater than that of the other working cases, which indicates that the wave height has a significant effect on the mechanical distribution of the stranded ship. The bending moment distribution of the ship is mainly affected by the load; however, the ground force and displacement can weaken the effect of the load to a certain extent. The differences in the negative maximum bending moment between the trough environment and the peak environment in each working case are 24.158 MN.m, 24.459 MN.m, −4.173 MN.m, and 70.616 MN.m, respectively. Under only wave cases, the maximum positive torque value at the longitudinal coordinate of −50 is more than twice the maximum positive torque value under the wind and wave cases.