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A 2-yr study was conducted to evaluate 1) the effects of nitrogen level from swine lagoon effluent on forage composition and animal performance and 2) the effects of supplemental escape protein (EP) on performance by steers grazing pastures fertilized with swine lagoon effluent. Bermudagrass (Cynodon dactylon) pastures were fertilized via sprinkler irrigation with either 448 or 896 kg/ha of N from swine lagoon effluent (two pasture replications/treatment). Within each pasture, four supplement treatments were evaluated using electronic Calan gates (8 steers.pasture-1.yr-1). The treatments were 1) negative control (pasture only); 2) energy control, which supplied 79.9 g of EP.hd-1.d-1; 3) 159 g of EP.hd-1.d-1, and 4) 239 g of EP.hd-1.d-1. Gains were increased (P < .05) an average of .15 kg/d by supplementation, with no differences in gain among supplements. Forage samples representative of that grazed by steers (CONSUM) and representative of all available forage (AVAIL) were obtained at 14-d intervals. Total CP content of CONSUM and AVAIL samples were slightly higher (P < .20 and P < .15, respectively) from pastures fertilized with 896 compared with 448 kg/ha of N (20.8 vs 20.0% for CONSUM and 15.2 vs 14.2% for AVAIL). Concentrations of nonprotein N and soluble true protein (% of CP) in both AVAIL and CONSUM samples were higher (P < .06) from pastures fertilized with the higher N level. Total N uptake by the plant, based on CP content and animal grazing days/ha, was essentially the same for both N treatments.

Four .8-ha pastures of bermudagrass (Cynodon dactylon [L.] Pers.) were fertilized with either 456 or 873 kg/ha of nitrogen (N) from swine lagoon effluent (two replicates per treatment) and grazed by steers over two summers. Within each pasture, steers received forage only, an energy source (corn), a mixture of corn and soybean meal, or a mixture of corn and blood meal via electronic Calan feeders. All supplements were offered at a level of 1.36 kg/d, and the soybean meal and blood meal supplements provided similar among quantities of protein. Weight gains were similar among supplemented steers, but supplemented steers gained faster (P < .05) than controls. Nitrogen fertilization level had no effect on steer gains, steer grazing days per hectare, or in vitro dry matter disappearance, NDF, and ADF of clipped forage samples. Plant protein and nitrate ion concentrations were greater (P < .06) in clipped forage samples receiving the higher N application rate. Nitrate ion concentrations were greater in available forage samples from the pastures with the high N application rate. Mean total N and nitrate N concentrations were similar in water samples obtained from monitoring wells for the two N treatments over the 2 yr and there were no year x N interactions. Chloride concentrations were greater (P < .05) and pH and specific conductance were less in water samples collected from the 873 kg than from the 456 kg/ha N treatment. Long-term studies are needed to examine the possible cumulative effects of applying various levels of swine waste to the same land area.

Several models and markers are available for digesta kinetic studies. In this study, kinetic estimates derived from chromium-mordanted hay or pellets were compared to estimates derived from rare earth markers (Yb, Dy, or Er) applied individually to samples. Twelve yearling rams (52 kg) were given ad libitum access to either hay or a commercial pelleted diet in a crossover experiment. Digesta kinetic estimates were obtained both by nonlinear analysis with two age-independent rates (G1G1) or with gamma time dependency in the fast compartment (G2G1 to G4G1) and by linear regression of natural log transformed fecal marker concentrations (LN method of estimate). Model did not influence total tract (P > .21) or ruminal mean retention times (P > .87). Partitioning of total retention time was similar (P > .21) for the LN, G3G1, and G4G1 models, but the G1G1 and G2G1 models did not adequately fit these data. Nonlinear models overestimated, and LN underestimated, fecal DM output by 9% (SEM = 4.7) for the hay diet. All the nonlinear models provided fecal DM output estimates that were within 5% of actual fecal DM output, but the LN model underestimated it by 18% (SEM = 3.3) for the pelleted diet. Ruminal outflow rate was slower (P < .01) and both ruminal and total mean retention time estimates were longer (P < .01) for Cr than for the rare earths. Despite the marker chosen, relative diet effects were similar. The three rare earth markers gave identical results for digesta kinetic estimates, indicating that they are useful for simultaneous study of more than one ingredient or particle, but direct comparison of rare earth and Cr-mordant passage rates is not advisable.

Twenty-four Holstein cows near peak lactation were fed diets formulated to contain 30% NDF with forage NDF from chopped wheat straw or chopped alfalfa hay in proportions of 0:3, 1:2, 2:1, and 3:0 in an 8-wk trial to evaluate effects of fiber source on lactational performance. Concentrate was based on highly degradable carbohydrate in steam-flaked sorghum grain. Forage to concentrate ratio decreased from 49:51 to 28:72 when straw was substituted for alfalfa hay. Milk yield (38.4 kg/d) and DMI (23.1 kg/d) were not influenced by forage source. Milk fat percentage decreased and milk protein percentage increased linearly with increasing straw. Yields of 3.5% FCM and fat showed linear and quadratic responses, because they appeared to be reduced only on the 0:3 diet, and protein yield showed a quadratic response and was highest for the 1:2 diet. Digestibility of NDF averaged 43.5, 45.4, 40.7, and 31.2%, and acetate to propionate ratio was 3.0, 2.4, 2.2, and 1.9 with increasing dietary straw. Passage rates were not influenced by diet and averaged 9.3, 7.6, 6.1, and 4.7%/h for fluid, grain, alfalfa hay, and wheat straw, respectively. It is suggested that the ratio of forage NDF to ruminally degradable starch be maintained greater than or equal to 1:1 when diets based on low quality forage are fed to cows in early lactation.

Three duodenally cannulated, lactating Holstein cows were dosed with rare earth-labeled grain to evaluate effects of passage model, sampling site, and marker dosing time on digesta passage parameters. Cows were given ad libitum access to feed twice daily. Rare earth-labeled grain (applied by the 24-h immersion technique) was fed immediately before (Dy) or 2 h after (Yb) the morning feeding, and duodenal digesta and feces were sampled. Marker excretion curves were fit to a two-compartment, biexponential model, using curve peeling or to a series of two-compartment models, with one to six orders of gamma time-dependency in the fast compartment, using nonlinear regression. Passage estimates from the curve-peeled, biexponential model were similar to those from the best fit of the nonlinear models, which had three orders of gamma time-dependency. Ruminal passage rate of grain, averaged across models, sampling site, and dosing time, was .077/h. Estimates of time to first appearance of marker at the sampling site and mean retention times were longer (8 vs 1 h and 25 vs 17 h, respectively), but passage rates were similar, when determined from fecal compared with duodenal samples (P less than .05). Marker dosing time did not influence any of the parameters. It is concluded that both curve-peeling (linear regression) and nonlinear regression methods can be equally useful for evaluating passage kinetics of grain in dairy cows and that sampling site and time of marker dosing have little effect on passage parameter estimates.

Roughage sources were compared in flaked milo-based diets that contained 35% chopped alfalfa hay (AH, control diet) or with cottonseed hulls (CSH) or chopped wheat straw (WS) replacing half the AH. Latin square experiments were used to measure total tract digestion coefficients, particulate passage rates (rare earths), liquid turnover rates (Co-EDTA), and rumination time in six growing steers (Exp. 1) and in situ digestion of DM and NDF, ruminal pH and ruminal DM distribution in three mature, ruminally cannulated steers (Exp. 2). Rates of passage from Exp. 1 and rates and extents of digestion from Exp. 2 were used to calculate apparent extent of ruminal digestion (AED). In Exp. 1, total tract digestibilities of DM and NDF were lower (P less than .05) by 7 and 22%, respectively, when CSH, but not WS, were included in the diet. Digestibility of cell solubles was not different (P greater than .10) among diets. Inclusion of WS increased (P less than .10) rumination time by 36%, and CSH increased intake (P less than .10) by 17% over the control diet. In Exp. 2, there tended to be (P less than .20) increased in situ digestion of milo and AH in the WS diet. Measures of ruminal pH were similar for all diets. The AED for AH and milo DM and NDF, and the proportion of total tract NDF digestion occurring in the rumen (50, 47 and 62% for control, CSH and WS diets, respectively), were highest (P less than .05) for the WS diet. This resulted in similar total tract digestibilities for the WS and AH diets. The two low-digestibility roughages had different effects at this concentrate level; wheat straw enhanced apparent extent of ruminal digestion for NDF of other ingredients in the mixed diets, but cottonseed hulls did not.

Six ruminally cannulated steers were used to determine the effects of altering dietary concentrates on fiber digestion. Diets contained 30, 60 or 90% of a concentrate based on flaked sorghum grain plus a 50:50 mixture of wheat straw and alfalfa hay. Total tract digestibility of NDF was not altered, but digestibility of potentially digested NDF (PDF) decreased (P less than .05) from 92 to 48% as concentrates increased from 30 to 90% of diet DM. Ruminal passage rate for straw (3.4 and 3.0%/h) and for hay (4.6 and 4.7%/h) was unchanged when concentrate was increased from 30 to 60%, but it decreased by 28 (2.2%/h) and 13% (4.1%/h), respectively (P less than .05), when concentrates were increased to 90%. Passage rate for grain (5.3, 5.1 and 4.4%/h) and fluid (9.3, 10.0 and 8.2%/h) was not influenced by concentrate percentage. Calculated ruminal digestibilities of NDF in individual ingredients did not differ between 30 and 60% concentrates, but they decreased (P less than .05) by 72, 57 and 34% for straw, hay and grain when concentrate was increased to 90%. Because of their relative contribution to total diet NDF, straw, hay and grain accounted for 28, 18 and 54% of the total decrease in estimated fiber digestion. It is concluded that PDF as well as NDF should be evaluated in studies of concentrate effects on fiber digestion, and that dietary concentrate level has more influence on passage rate of low-quality forage than on passage rate of grain or high-quality forage. In 90% concentrate diets, although fiber digestibility was depressed more for forage than for grain, grain accounted for most of the depression in fiber digestion because grain was the primary source of dietary fiber.