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977 results on '"Pinzani, M"'

64. Collagen proportionate área is an independent predictor of long term outcome in patients with alcoholic liver disease

Author

Misas, M.G., primary, Koutsoumarakis, A., additional, Huang, Y., additional, Thiele, M., additional, Hall, A., additional, Covelli, C., additional, Buzzetti, E., additional, Prat, L.I., additional, Roccarina, D., additional, Detlefsen, S., additional, Luong, T.V., additional, Jeffrey, G., additional, Pinzani, M., additional, and Tsochatzis, E., additional

Published
2018
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70. Genetic association analysis identifies variants associated with disease progression in primary sclerosing cholangitis

Author

Alberts, R, De Vries, E, Goode, E, Jiang, X, Sampaziotis, F, Rombouts, K, Böttcher, K, Folseraas, T, Weismüller, T, Mason, A, Wang, W, Alexander, G, Alvaro, D, Bergquist, A, Björkström, N, Beuers, U, Björnsson, E, Boberg, K, Bowlus, C, Bragazzi, M, Carbone, M, Chazouillères, O, Cheung, A, Dalekos, G, Eaton, J, Eksteen, B, Ellinghaus, D, Färkkilä, M, Festen, E, Floreani, A, Franceschet, I, Gotthardt, D, Hirschfield, G, Hoek, B, Holm, K, Hohenester, S, Hov, J, Imhann, F, Invernizzi, P, Juran, B, Lenzen, H, Lieb, W, Liu, J, Marschall, H, Marzioni, M, Melum, E, Milkiewicz, P, Müller, T, Pares, A, Rupp, C, Rust, C, Sandford, R, Schramm, C, Schreiber, S, Schrumpf, E, Silverberg, M, Srivastava, B, Sterneck, M, Teufel, A, Vallier, L, Verheij, J, Vila, A, Vries, B, Zachou, K, Chapman, R, Manns, M, Pinzani, M, Rushbrook, S, Lazaridis, K, Franke, A, Anderson, C, Karlsen, T, Ponsioen, C, Weersma, R, Alberts, R, De Vries, E, Goode, E, Jiang, X, Sampaziotis, F, Rombouts, K, Böttcher, K, Folseraas, T, Weismüller, T, Mason, A, Wang, W, Alexander, G, Alvaro, D, Bergquist, A, Björkström, N, Beuers, U, Björnsson, E, Boberg, K, Bowlus, C, Bragazzi, M, Carbone, M, Chazouillères, O, Cheung, A, Dalekos, G, Eaton, J, Eksteen, B, Ellinghaus, D, Färkkilä, M, Festen, E, Floreani, A, Franceschet, I, Gotthardt, D, Hirschfield, G, Hoek, B, Holm, K, Hohenester, S, Hov, J, Imhann, F, Invernizzi, P, Juran, B, Lenzen, H, Lieb, W, Liu, J, Marschall, H, Marzioni, M, Melum, E, Milkiewicz, P, Müller, T, Pares, A, Rupp, C, Rust, C, Sandford, R, Schramm, C, Schreiber, S, Schrumpf, E, Silverberg, M, Srivastava, B, Sterneck, M, Teufel, A, Vallier, L, Verheij, J, Vila, A, Vries, B, Zachou, K, Chapman, R, Manns, M, Pinzani, M, Rushbrook, S, Lazaridis, K, Franke, A, Anderson, C, Karlsen, T, Ponsioen, C, and Weersma, R

Abstract

Objective Primary sclerosing cholangitis (PSC) is a genetically complex, inflammatory bile duct disease of largely unknown aetiology often leading to liver transplantation or death. Little is known about the genetic contribution to the severity and progression of PSC. The aim of this study is to identify genetic variants associated with PSC disease progression and development of complications. Design We collected standardised PSC subphenotypes in a large cohort of 3402 patients with PSC. After quality control, we combined 130 422 single nucleotide polymorphisms of all patients - obtained using the Illumina immunochip - with their disease subphenotypes. Using logistic regression and Cox proportional hazards models, we identified genetic variants associated with binary and time-to-event PSC subphenotypes. Results We identified genetic variant rs853974 to be associated with liver transplant-free survival (p=6.07×10 -9). Kaplan-Meier survival analysis showed a 50.9% (95% CI 41.5% to 59.5%) transplant-free survival for homozygous AA allele carriers of rs853974 compared with 72.8% (95% CI 69.6% to 75.7%) for GG carriers at 10 years after PSC diagnosis. For the candidate gene in the region, RSPO3, we demonstrated expression in key liver-resident effector cells, such as human and murine cholangiocytes and human hepatic stellate cells. Conclusion We present a large international PSC cohort, and report genetic loci associated with PSC disease progression. For liver transplant-free survival, we identified a genome-wide significant signal and demonstrated expression of the candidate gene RSPO3 in key liver-resident effector cells. This warrants further assessments of the role of this potential key PSC modifier gene.

Published
2018

75. Optimization of Liver Decellularization Maintains Extracellular Matrix Micro-Architecture and Composition Predisposing to Effective Cell Seeding

Author

Maghsoudlou, P., Georgiades, F., Smith, H., Milan, A., Shangaris, P., Urbani, L., Loukogeorgakis, S.P., Lombardi, B., Mazza, G., Hagen, C., Sebire, N.J., Turmaine, M., Eaton, S., Olivo, A., Godovac-Zimmermann, J., Pinzani, M., Gissen, P., and De Coppi, P.

Subjects
Proteomics, Histology, lcsh:Medicine, Surgical and Invasive Medical Procedures, Biochemistry, Digestive System Procedures, Tandem Mass Spectrometry, Medicine and Health Sciences, Animals, Humans, lcsh:Science, Transplantation, Tissue Scaffolds, lcsh:R, Biology and Life Sciences, Proteins, Organ Transplantation, Cell Biology, Hep G2 Cells, Elastin, Liver Transplantation, Extracellular Matrix, Rats, FRELON CAMERA, Liver, Cardiovascular Anatomy, Microscopy, Electron, Scanning, Blood Vessels, lcsh:Q, Anatomy, Cellular Structures and Organelles, Peptides, Collagens, Research Article, Chromatography, Liquid
Abstract

Hepatic tissue engineering using decellularized scaffolds is a potential therapeutic alternative to conventional transplantation. However, scaffolds are usually obtained using decellularization protocols that destroy the extracellular matrix (ECM) and hamper clinical translation. We aim to develop a decellularization technique that reliably maintains hepatic microarchitecture and ECM components. Isolated rat livers were decellularized by detergent-enzymatic technique with (EDTA-DET) or without EDTA (DET). Histology, DNA quantification and proteomics confirmed decellularization with further DNA reduction with the addition of EDTA. Quantification, histology, immunostaining, and proteomics demonstrated preservation of extracellular matrix components in both scaffolds with a higher amount of collagen and glycosaminoglycans in the EDTA-DET scaffold. Scanning electron microscopy and X-ray phase contrast imaging showed microarchitecture preservation, with EDTA-DET scaffolds more tightly packed. DET scaffold seeding with a hepatocellular cell line demonstrated complete repopulation in 14 days, with cells proliferating at that time. Decellularization using DET preserves microarchitecture and extracellular matrix components whilst allowing for cell growth for up to 14 days. Addition of EDTA creates a denser, more compact matrix. Transplantation of the scaffolds and scaling up of the methodology are the next steps for successful hepatic tissue engineering.

Published
2016

78. Role of the stromal-derived factor-1 (SDF-1)-CXCR4 axis in the interaction between hepatic stellate cells and cholangiocarcinoma

Author

Gentilini, A, Rombouts, K, Galastri, S, Caligiuri, A, Mingarelli, E, Mello, T, Marra, F, Mantero, S, Roncalli, M, Invernizzi, P, Pinzani, M, Pinzani, M., INVERNIZZI, PIETRO, Gentilini, A, Rombouts, K, Galastri, S, Caligiuri, A, Mingarelli, E, Mello, T, Marra, F, Mantero, S, Roncalli, M, Invernizzi, P, Pinzani, M, Pinzani, M., and INVERNIZZI, PIETRO

Abstract

Backgrounds & Aims: Cholangiocarcinoma (CCA) is highly fatal because of early invasion, widespread metastasis, and lack of an effective therapy. Migration, invasion, and metastasis of CCA cells are modulated by signals received from stromal cells. The SDF-1-CXCR4 axis emerges as a pivotal regulator of migration and survival of different tumor cells. The aim of the present study was to characterize the interaction between CCA cells and human hepatic stellate cells (hHSC) focusing on the role of SDF-1. Methods: The intrahepatic CCA cell line HuCCT-1 and primary hHSC were used for this study. RNA expression was examined by RTQ-PCR and protein expression by Western blotting. Immunofluorescence microscopy and immunohistochemistry were also employed. Migration of CCA cells was assessed using modified Boyden chambers. Results: CXCR4 was clearly expressed in CCA cells of human CCA liver specimens. SDF-1 and hHSC conditioned medium (CM) promoted HuCCT-1 cell migration, which was abrogated by pre-incubation with AMD3100, a non-peptide antagonist of the CXCR4 receptor. In addition, HuCCT-1 cells silenced for CXCR4 did not migrate in presence of SDF-1. Both P-ERK and p-AKT were implicated in HuCCT-1 migration and showed a biphasic trend under stimulation of SDF-1. Finally, SDF-1 induced apoptotic rescue of HuCCT-1 cells by binding to CXCR4. Conclusions: Our study demonstrates that CCA cells migration and survival are modulated by the crosstalk between SDF-1, released by hHSC, and HuCCT-1 cells bearing CXCR4. © 2012 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved

Published
2012

80. Liver stiffness, a non-invasive marker of liver disease: a core study group report

Author

Bonino, F, Arena, U, Brunetto, Mr, Coco, B, Fraquelli, M, Oliveri, F, Pinzani, M, Prati, D, Rigamonti, C, Vizzuti, F, Collaborators: Balocco M, Liver Stiffness Study Group 'Elastica' of the Italian Association for the Study of the Liver., Forni, G, Bottelli, R, Bruno, R, Filice, C, Bruno, S, Cammà, C, Craxì, A, Colletta, C, Colombatto, P, Colombo, M, Fatuzzo, F, Larocca, L, Montineri, A, Festi, D, Colecchia, A, Grasso, A, Maida, I, Mazzotta, F, Marino, N, Blanc, P, Puoti, M, Quartini, M, Sacchini, D, Vinelli, F., Craxi, A, Bonino, F, Arena, U, Brunetto, MR, Coco, B, Fraquelli, M, Oliveri, F, Pinzani, M, Prati, D, Rigamonti, C, Vizzuti, F, and Liver Stiffness Study Group 'Elastica' of the Italian Association for the Study of the Liver

Subjects
Pharmacology, Liver Cirrhosis, medicine.medical_specialty, Core (anatomy), business.industry, Non invasive, Hepatitis B, medicine.disease, Gastroenterology, Clinical Practice, liver stiffness, Liver disease, Infectious Diseases, Liver, Liver stiffness, Internal medicine, medicine, Elasticity Imaging Techniques, Pharmacology (medical), Transient elastography, business, Liver pathology, Biomarkers
Abstract

The ability to evaluate liver stiffness non-invasively in clinical practice by measuring transient elastography using FibroScan® has resulted in considerable interest and enthusiasm. A core study group, organized by the Italian Association for the Study of the Liver, has assessed the usefulness of FibroScan® in the diagnosis and management of liver disease in clinical practice. The group concluded that FibroScan® is a valuable, non-invasive technique and have developed a consensus report form for registering transient elastography results. In this article, we report the findings of the study group.

Published
2010

87. A new in vitro hepatocellular carcinoma model based on human normal and fibrotic 3D extracellular matrix scaffold bio-engineering

Author

Telese, A., primary, Mazza, G., additional, Longato, L., additional, Al-Akkad, W., additional, Frenguelli, L., additional, Marrone, G., additional, Tamburrino, D., additional, Spoletini, G., additional, Hall, A., additional, Luong, T.V., additional, Moore, K., additional, Malagò, M., additional, Pinzani, M., additional, and Rombouts, K., additional

Published
2017
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91. Validation of the Baveno VI elastography criteria for the definition of compensated advanced chronic liver disease: an individual patient meta-analysis

Author

Tsochatzis, E., primary, Hiriart, J.B., additional, Lupsor-Platon, M., additional, Bronte, F., additional, Boursier, J., additional, Marra, F., additional, Payance, A., additional, Brodkin, E., additional, Castera, L., additional, Papatheodoridis, G., additional, Arena, U., additional, Cales, P., additional, Calvaruso, V., additional, de Ledinghen, V., additional, and Pinzani, M., additional

Published
2017
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