51. The functional class evaluated in rheumatoid arthritis is associated with soluble TGF-β1 serum levels but not with G915C (Arg25Pro) TGF-β1 polymorphism.
- Author
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Muñoz-Valle JF, Torres-Carrillo NM, Guzmán-Guzmán IP, Torres-Carrillo N, Ruiz-Quezada SL, Palafox-Sánchez CA, Rangel-Villalobos H, Ramírez-Dueñas MG, Parra-Rojas I, Fafutis-Morris M, Bastidas-Ramírez BE, and Pereira-Suárez AL
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Arthritis, Rheumatoid ethnology, Female, Genotype, Humans, Male, Mexico ethnology, Middle Aged, Transforming Growth Factor beta1 classification, Young Adult, Amino Acid Substitution genetics, Arthritis, Rheumatoid classification, Arthritis, Rheumatoid genetics, Polymorphism, Single Nucleotide genetics, Transforming Growth Factor beta1 blood, Transforming Growth Factor beta1 genetics
- Abstract
The influence of genetic factors in rheumatoid arthritis (RA) has been described, including several cytokine genes such as transforming growth factor β (TGF-β) with regulatory effects on lymphocytes, dendritic cells, macrophages, chondrocytes, and osteoblasts, which are important in the RA pathogenesis. The G915C TGF-β1 polymorphism has been associated with soluble TGF-β1 (sTGF-β) serum levels. Thus, we studied the association of G915C (Arg25Pro) TGF-β1 polymorphism with sTGF-β1 serum levels in RA. We enrolled 120 RA patients and 120 control subjects (CS). The G915C TGF-β1 polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, and sTGF-β1 serum levels were quantified using an ELISA kit. The genotype frequency of G915C TGF-β1 polymorphism in RA and CS was G/G (91.7%), G/C (8.3%), C/C (0%) and G/G (85.8%), G/C (14.2%), C/C (0%), respectively, without significant differences. Moreover, the G/G TGF-β1 genotype carriers presented the highest disability index evaluated for the Spanish HAQ-DI score (P < 0.001). In addition, the sTGF-β1 serum levels were higher in RA (182.2 ng/mL) than CS (160.2 ng/mL), there was not significant difference. However, we found a positive correlation between the sTGF-β1 serum levels and the functional class (r = 0.472, P = 0.023). In conclusion, the G915C (Arg25Pro) TGF-β1 polymorphism is not associated with RA, but the sTGF-β1 serum levels are related with the functional class in RA.
- Published
- 2012
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