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The copolymerizations of N-vinylpyrrolidone with N-hydroxyphthalimide and N-hydroxysuccinimide esters of acrylic, methacrylic and crotonic acids have been investigated. The reactivity ratios were determined, and kinetic investigations of the copolymerizations were carried out. It was shown that, by varying the acid component of the monomeric ester, it is possible to obtain reactive copolymers greatly differing in structure.

LC on short monolithic columns (Convective Interaction Medium Disks) was applied to investigate several specially synthesized water soluble polycations of different charge type (primary, tertiary, quaternary amine), as well as a copolymer of neutral saccharide and cationic monomers, regarding their ability to form reversible complexes with DNA. For this purpose, two separation modes were used, namely, pseudo-affinity and cation-exchange chromatography. Synthetic polynucleotides, namely, polyriboadenylic acid (poly(rA)) and polyribocytidylic acid (poly(rC)), were used as approximate structural analogues of DNA. In first case, the hypothetical specific binding between dissolved polymers and polynucleotide (poly(rA) or poly(rC)), covalently attached to epoxy-bearing monolithic sorbent, has been studied and compared to the results obtained using cation exchange chromatography. Quantitative parameters of interactions between macromolecules were established using frontal elution method.

The dynamic light scattering method was used to study the process of complex formation of cholesterol-containing polymers of vinylsaccharide 2-deoxy-2-methacrylamido-D-glucose (double and ternary copolymers) in water solutions in the different polymer mole ratio. Compared to the initial ternary cholesterol-containing copolymer the conditions of compaction of the complex formed are found.