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52. Enhanced DNA Cleavage by Mercury(II) Porphyrin at a Low Concentration of HaeIII Restriction Enzyme

Author

Nyarko, Elvis, Tabata, Masaaki, and Watanabe, Keiichi

Abstract

Mercury(II) porphryin enhanced DNA cleavage in the presence of 0.2 units µL−1of HaeIII at which concentration the restriction enzyme could not cleave DNA in the absence of the porphyrin. This was ascribed to the synergistic effect of the bound Hg2+ions to DNA and the intercalated free base porphyrin, released from the mercury(II) porphyrin complex upon binding to DNA, which was confirmed by UV–visible and CD spectroscopic measurements.

Published
2001
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