51. Dynamics of fatty acid regulatory genes during ovarian development in Penaeus monodon
- Author
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Nitsara Karoonuthaisiri, Punsa Tobwor, Rungnapa Leelatanawit, Somjai Wongtriphop, Vanicha Vichai, Wananit Wimuttisuk, Jutatip Khudet, and Pacharawan Deenarn
- Subjects
0301 basic medicine ,Ablation Techniques ,Embryology ,Time Factors ,Eyestalk ablation ,Ovary ,Biology ,Gene Expression Regulation, Enzymologic ,Penaeus monodon ,Andrology ,03 medical and health sciences ,Endocrinology ,Penaeidae ,medicine ,Animals ,Regulator gene ,Regulation of gene expression ,chemistry.chemical_classification ,Fatty Acids ,Obstetrics and Gynecology ,Fatty acid ,Gene Expression Regulation, Developmental ,Polychaeta ,Cell Biology ,biology.organism_classification ,Lipid Metabolism ,Animal Feed ,Shrimp ,030104 developmental biology ,medicine.anatomical_structure ,Reproductive Medicine ,chemistry ,Hepatopancreas ,Female - Abstract
The delay in ovarian maturation in farmed black tiger shrimp Penaeus monodon has resulted in the widespread practice of feeding broodstock with the polychaetes Perinereis nuntia and their unilateral eyestalk ablation. Although this practice alters fatty acid content in shrimp ovaries and hepatopancreas, its effects on fatty acid regulatory genes have yet to be systematically examined. Here, microarray analysis was performed on hepatopancreas and ovary cDNA collected from P. monodon at different ovarian maturation stages, revealing that 72 and 58 genes in fatty acid regulatory pathways were differentially expressed in hepatopancreas and ovaries respectively. Quantitative real-time PCR analysis revealed that ovarian maturation was associated with higher expression levels of acetyl-CoA acetyltransferase, acyl-CoA dehydrogenase, acyl-CoA oxidase 3 and long-chain fatty acid transport protein 4 in hepatopancreas, whereas the expression levels of 15 fatty acid regulatory genes were increased in shrimp ovaries. To distinguish the effects of different treatments, transcriptional changes were examined in P. monodon with stage 1 ovaries before polychaete feeding, after one-month of polychaete feeding and after eyestalk ablation. Polychaete feeding resulted in lower expression levels of enoyl-CoA hydratase and acyl-CoA synthetase medium-chain family member 4, while the expression level of phosphatidylinositide phosphatase SAC1 was higher in shrimp hepatopancreas and ovaries. Additionally, eyestalk ablation resulted in a higher expression level of long-chain fatty acid-CoA ligase 4 in both tissues. Together, our findings describe the dynamics of fatty acid regulatory pathways during crustacean ovarian development and provide potential target genes for alternatives to eyestalk ablation in the future.
- Published
- 2018