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Inheritance of MYMIV tolerance was determined in two sets of recombinant inbred lines (RILs) of mungbean. The two sets comprising 143 and 79 RILs each derived from the cross between PM5 x Sub2 and B1 X Sub2, respectively were considered for the study. Sub2 was a Sublobata derived lines whereas, B1 was a popular small seeded cultivar of west Bengal, susceptible against MYMIV. It was observed that one or two major genes with a few modifiers played a significant role in resistance mechanism against MYMIV in the lower Gangetic alluvial zone. Resistance alleles for Sub2 and PM5 are probably allelic as observed from frequency distribution pattern. A set of 177 SSRs were employed for identification of polymorphism between parents. Only 37 SSRs showed polymorphism between Sublobata and B1 or PM5. But only eight SSRs were polymorphic between two high yielding cultivars. Markers linked with MYMIV tolerance like RGA, SCAR and others from earlier studies were also considered and found that only one RGA derived marker showed polymorphism between resistance and susceptible parents.

A novel protocol for enhanced in vitro multiple shoot induction was developed for Anthurium andreanum Lind. First shoot bud was induced within a week in MS supplemented with 0.1 mg/l NAA and 0.25 mg/l BAP where maximum six buds from single apical bud explant appeared within next 20 days. For multiple shoot proliferation MS with 0.5 mg/l BAP and 60 mg/l adenine sulphate (ADS) proved to be best resulting ten multiple shoots per inoculated shoot bud within 50 days. As many as 11 roots per plantlet were produced in 27 days using MS with 0.5 mg/l IAA and 2 g/l activated charcoal. Autoclaved sand and intermittent water spraying optimized the primary hardening period of 15 days and then earthen pots filled with sand, soil, charcoal and coconut fibre ensured the recovery of 51 well hardened plantlets out of 60 in next 30 days showing 85% success. The genetic identity of both ex vitro hardened clones and in vitro sustained clones with their mother ant were tested using 10 ISSR primers, displayed no polymorphism. Key words: Anthurium, In vitro cloning, Multiple shoot, Genetic identity D.O.I. 10.3329/ptcb.v18i2.3361 Plant Tissue Cult. & Biotech. 18 (2): 113-122, 2008 (December)