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605 results on '"Naderi-Manesh, Hossein"'

61. Bright Green Light-Emitting Gold Nanoclusters Confined in Insulin as Selective Fluorescent Switch Probes for Sensing and Imaging of Copper Ions and Glutathione.

Author

Shamsipur, Mojtaba, Babaee, Elaheh, Gholivand, Mohammad-Bagher, Molaabasi, Fatemeh, Mousavi, Farimah, Barati, Ali, Hajipour Verdom, Behnam, Shojaedin-Givi, Behnaz, and Naderi-Manesh, Hossein

Abstract

In this study, an on–off–on fluorescence probe is developed for rapid, sensitive, and selective detection of Cu2+ ions and glutathione (GSH) based on green light-emitting insulin-capped gold nanoclusters (Ins/Au-NCs). Ins/Au-NCs with low toxicity and excellent stability were prepared by utilizing a simple and green strategy. Cu2+ ions can selectively decrease the fluorescence intensity of Ins/Au-NCs by an aggregation-caused quenching mechanism, which can be recovered in the presence of GSH. Under the optimized condition, the detection limits for determination of Cu2+ ions and GSH are estimated as 7.5 and 9.2 nM, with linear ranges of 0.05 to 1.7 μM and 0.05 to 1.5 μM, respectively. Chemical modeling was also employed to investigate the interaction of the Ins/Au-NCs probe with Cu2+, Hg2+, glutathione, and cysteine. The calculated molecular orbital energy well confirmed the experimental selective results obtained. Luminescence cellular imaging exhibited a sensitive response of the Ins/Au-NCs probe toward Cu2+ ions in breast cancer cells MCF-7, and thus, it potentially acted as an intracellular GSH probe in MCF-7 compared to the human fibroblast HFF cells. The as-prepared probe was successfully applied to low levels of Cu2+ ions and GSH in water and human serum samples. The designed Ins/Au-NCs-based probe may also provide a biocompatible platform for label-free GSH detection in biological and clinical implications that comprise staining and early prevention of cancer, AIDS, and Alzheimer's and cardiovascular diseases. [ABSTRACT FROM AUTHOR]

Published
2023
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66. List of contributors

Author

Abishek, K.G., Abnous, Khalil, Adewale, Olusola Bolaji, Aguilar-Aguilar, Angélica, Ahmed, Marya, Akturk, Omer, Alam, Mohammad Sarwar, Almalki, Waleed H., Anadozie, Scholastica Onyebuchi, Anegundi, Raghavendra Vamsi, Arumuganainar, Deepavalli, Casagrande, Laura De Roch, Conte-Junior, Carlos Adam, Dandela, Rambabu, Daunert, Sylvia, Davids, Hajierah, De Oliveira, Jade, Dogan, Nesrin, El-Kholy, Abdullah Ibrahim, Fadel, Maha, Farfán-Castro, Susan, Ford, John Chetley, García-Soto, Mariano J., González-Ballesteros, N., González-Ortega, Omar, Hara, Daiki, Hashemzadeh, Hadi, Iftikhar, Fizza, Kaur, Gurleen, Kesharwani, Prashant, Khoshbin, Zahra, Krishnamurthi, Indhu, Kumar, Pawan, Manu, K.R., Medina-Cruz, David, Mishra, Vineet Kumar, Mostafavi, Ebrahim, Naderi-Manesh, Hossein, Polack, Alan, Prakash, Kishan, Qazi, Rida e Maria, Ramadoss, Ramya, Rath, Jyotisman, Rehman, Fawad Ur, Rey-Méndez, R., Rodríguez-Argüelles, M.C., Rosales-Mendoza, Sergio, Rout, Smruti Rekha, Roux, Saartjie, Sahebkar, Amirhossein, Sajid, Zahra, Saravanan, Muthupandian, Schmidt, Ryder, Shi, Junwei, Silveira, Gustavo De Bem, Silveira, Paulo Cesar Lock, Soltani, M., Souri, Mohammad, Taghdisi, Seyed Mohammad, Tao, Wensi, Tessaro, Leticia, Truong, Linh B., Venturini, Ligia Milanez, Vieira, Italo Rennan Sousa, Wheate, Nial J., Yadalam, Pradeep Kumar, Yadav, Harlokesh Narayan, Yang, Yupin, Yilmaz, Bengi, Zahraee, Hamed, and Zhao, Chunqiu

Published
2024
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83. PDMS Nano-Modified Scaffolds for Improvement of Stem Cells Proliferation and Differentiation in Microfluidic Platform

Author

Hashemzadeh, Hadi, Allahverdi, Abdollah, Sedghi, Mosslim, Vaezi, Zahra, Tohidi Moghadam, Tahereh, Rothbauer, Mario, Fischer, Michael Bernhard, Ertl, Peter, and Naderi-Manesh, Hossein

Subjects
lcsh:Chemistry, microfluidics material, lcsh:QD1-999, nano biointerface, stem cells, nanoparticle-polymer composites, nanotopography, technology, industry, and agriculture, osteogenic differentiation, macromolecular substances, PDMS functionalization, Article, nanomaterials
Abstract

Microfluidics cell-based assays require strong cell-substrate adhesion for cell viability, proliferation, and differentiation. The intrinsic properties of PDMS, a commonly used polymer in microfluidics systems, regarding cell-substrate interactions have limited its application for microfluidics cell-based assays. Various attempts by previous researchers, such as chemical modification, plasma-treatment, and protein-coating of PDMS revealed some improvements. These strategies are often reversible, time-consuming, short-lived with either cell aggregates formation, not cost-effective as well as not user- and eco-friendly too. To address these challenges, cell-surface interaction has been tuned by the modification of PDMS doped with different biocompatible nanomaterials. Gold nanowires (AuNWs), superparamagnetic iron oxide nanoparticles (SPIONs), graphene oxide sheets (GO), and graphene quantum dot (GQD) have already been coupled to PDMS as an alternative biomaterial enabling easy and straightforward integration during microfluidic fabrication. The synthesized nanoparticles were characterized by corresponding methods. Physical cues of the nanostructured substrates such as Young&rsquo, s modulus, surface roughness, and nanotopology have been carried out using atomic force microscopy (AFM). Initial biocompatibility assessment of the nanocomposites using human amniotic mesenchymal stem cells (hAMSCs) showed comparable cell viabilities among all nanostructured PDMS composites. Finally, osteogenic stem cell differentiation demonstrated an improved differentiation rate inside microfluidic devices. The results revealed that the presence of nanomaterials affected a 5- to 10-fold increase in surface roughness. In addition, the results showed enhancement of cell proliferation from 30% (pristine PDMS) to 85% (nano-modified scaffolds containing AuNWs and SPIONs), calcification from 60% (pristine PDMS) to 95% (PDMS/AuNWs), and cell surface marker expression from 40% in PDMS to 77% in SPION- and AuNWs-PDMS scaffolds at 14 day. Our results suggest that nanostructured composites have a very high potential for stem cell studies and future therapies.

Published
2020

91. Improvement of anti-biofilm activities via co-delivery of curcumin and gentamicin in lipid-polymer hybrid nanoparticle.

Author

Sadeghi Mohammadi, Sanam, Vaezi, Zahra, and Naderi-Manesh, Hossein

Subjects
GENTAMICIN, CURCUMIN, ULTRAVIOLET spectroscopy, TRANSMISSION electron microscopy, ANTIBACTERIAL agents, FLUORESCENCE spectroscopy, EXOTOXIN
Abstract

Pseudomonas aeruginosa is the most common pathogen that causes chronic lung infections and recurrence of the disease in cystic fibrosis patients by hiding inside cells and biofilm matrix. Herein, we developed gentamicin and curcumin-loaded lipid-polymer hybrid nanoparticle- (termed CG-HNPs) to evaluate in vitro activities against biofilm-embedded P. aeruginosa and compared with lipid nanoparticles containing the same drugs (CG-Lip). The nanoparticles were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), fluorescence spectroscopy, and ultraviolet-visible (UV-vis) spectroscopy, which demonstrated that HNPs with a diameter of approximately 340 nm were uniform. The optimal CG-HNPs formulation illustrated high encapsulation (∼70%) and controlled release characteristics (gradually released in 72 h). The antibacterial activities of generated nanoparticles are maintained against planktonic and biofilm bacteria and it is effective in damage established biofilms. Besides, HNPs were biocompatible and nontoxic to J774 and HFF cell lines and uptake by the macrophages (J774), which facilitated the killing of intracellular bacteria in macrophages. These results introduced CG-HNPs as a promising antibacterial agent for the treatment of chronic infections and intracellular bacteria due to excellent antibacterial activity. [ABSTRACT FROM AUTHOR]

Published
2022
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