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Specific staining of the extracellular matrix components is especially helpful in studying tissue remodeling, particularly in the case of connective tissue pathologies. As developed by Junqueira and colleagues in 1979, specific staining by Picrosirius red is one of the most important stains to study collagen networks in different tissues. Under polarized light, collagen bundles appear green, red or yellow, and are easily differentiated from the black background, thus allowing for quantitative morphometric analysis. As Junqueira and colleagues point out, many studies use color staining to differentiate collagen bundles and to specify collagen types, yet other studies report that polarized colors only reflect fiber thickness and packing. Using a simple histological example, our study illustrates the inability of Picrosirius red staining to differentiate collagen types, since the absorbed amount of polarized light by this dye strictly depends on the orientation of the collagen bundles. [ABSTRACT FROM AUTHOR]

Background: The aim of this retrospective clinical study was to compare the complications after the harvest of bone from the iliac crest and the calvarium. Materials and Methods: One hundred and eighty patients were selected for this study (125 iliac crest and 55 calvarium donor sites). Data were collected from the medical records and classified as major or minor complications for each procedure. Results: The highest percentage of total complications (66.4%) was recorded when the iliac crest was harvested. The highest percentage of major complications was associated with the harvest of calvarium site (14.5%), while highest percentage of minor complications was associated with the harvest of the iliac site (60.8%). Conclusion: Harvesting of the iliac crest was associated with the highest percentage of complications. Major complications were more observed with harvesting of the calvarium. [ABSTRACT FROM AUTHOR]

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Violation of the biologic width could induce different periodontal consequences depending on many factors such as the type of the restorative material used. In this study, the histological performance and the marginal adaptation of three filling materials (amalgam, composite resin and compomer) are evaluated after a period of three months. Class V cavities with apical margin located at the alveolar bone crest were prepared on Pointer dogs teeth and restored with these three materials. Histological analysis showed that lesions in epithelial attachment were mainly noticed with amalgam fillings and the scale of inflammatory cells was the highest when amalgam was used. Compomer showed the best marginal adaptation.

Amalgam, composite resin and compomer are frequently used in operative dentistry today. Earlier studies have shown the periodontal consequences induced by these materials if they have intracrevicular restorative margins. This study was performed on dogs where Class V cavities with apical margin located at the alveolar bone crest were prepared and restored with these materials. Three months later, the experimental and control sites were prepared for histological analysis. Mean bone loss was significant only with compomer. The mean length of the supracrestal connective tissue was almost the same with the three fillings. The length of epithelial attachment was the highest with composites. There was a decrease in the length of the biologic width from Day 0 to Day 90.