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51. High Biofilm-Forming Ability and Clonal Dissemination among Colistin-Resistant Escherichia coli Isolates Recovered from Cows with Mastitis, Diarrheic Calves, and Chickens with Colibacillosis in Tunisia

Author

Dhaouadi, Sana, primary, Romdhani, Amel, additional, Bouglita, Wafa, additional, Chedli, Salsabil, additional, Chaari, Soufiene, additional, Soufi, Leila, additional, Cherif, Ameur, additional, Mnif, Wissem, additional, Abbassi, Mohamed Salah, additional, and Elandoulsi, Ramzi Boubaker, additional

Published
2023
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63. Antioxidant and hepatoprotective effects of novel heteropolysaccharide isolated from Lobularia maritima on CCl4‐induced liver injury in rats

Author

Ben Hsouna, Anis, primary, Hfaiedh, Mbarka, additional, Ben Slima, Sirine, additional, Romdhane, Walid Ben, additional, Akacha, Boutheina Ben, additional, Bouterra, Mohamed Taieb, additional, Dhifi, Wissal, additional, Mnif, Wissem, additional, Brini, Faical, additional, Ben Saad, Rania, additional, and Ben Salah, Riadh, additional

Published
2022
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64. Design and use of chimeric peptides in a new non‐destructive ecological process applied to the extraction of all trans/9‐cis β‐carotene isomers from Dunaliella salina

Author

Kouidhi, Soumaya, primary, Mnif, Wissem, additional, Alqarni, Nada, additional, Abdelwahed, Soukaina, additional, Redissi, Alaeddine, additional, Ammous, Nihel, additional, Selmi, Boulbaba, additional, Gargouri, Ali, additional, Achour, Sami, additional, Cherif, Ameur, additional, and Mosbah, Amor, additional

Published
2022
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67. Immunomodulatory effect of Linalool (Lin) against CCl4‐induced hepatotoxicity and oxidative damage in rats.

Author

Hsouna, Anis Ben, Sadaka, Carmen, Beyrouthy, Marc El, Hfaiedh, Mbarka, Dhifi, Wissal, Brini, Faical, Saad, Rania Ben, and Mnif, Wissem

Subjects
LINALOOL, HEPATOTOXICOLOGY, CARBON tetrachloride, RATS, INTERLEUKIN-10, HISTOPATHOLOGY, OLIVE oil
Abstract

The current study explored the hepatoprotective and immunomodulatory effects of Linalool (Lin) against carbon tetrachloride (CCl4)‐induced toxicity in mice. Four study groups (n = 8 each) were used: (1) a negative control group and (2) a toxicity control group (single dose of CCl4 administered on day 14 as 1 mL/kg of CCL4 in 1% olive oil). Intraperitoneally (i.p.)), and two experimental groups where mice were treated with either (3) Lin (25 mg/kg b.w., orally, daily for 15 days) or (4) pretreated with Lin (25 mg/kg b.w., orally, daily for 14 days) and intoxicated with CCl4 (1 mL/kg of CCL4 in 1% olive oil. i.p.) on day 14. The levels of the anti‐inflammatory cytokine interleukin 10 (IL‐10), the proinflammatory cytokines TNF‐α, IL‐6, and TGF‐1β, and the histopathology of the liver were assessed. According to our findings, IL‐10 concentrations were significantly increased in Lin‐treated groups, while other cytokine levels were marked by a considerable decrease in the toxicity model group (CCl4‐treated group). Histopathological examinations of liver tissues showed that the Lin‐treated groups had an almost normal structure. The current findings showed that Lin could inhibit CCl4‐induced liver injury in mice, which warrants further investigation of Lin as a potential protective and therapeutic agent against hepatotoxicity. [ABSTRACT FROM AUTHOR]

Published
2023
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68. Microassay validation for bacterial IAA estimation as a new fine-tuned PGPR screening assay.

Author

Abdelwahed, Soukaina, Cherif, Hanen, Bejaoui, Bilel, Saadouli, Ilhem, Hajji, Tarek, Ben Halim, Nizar, Ouertani, Awatef, Ouzari, Imen, Cherif, Ameur, Mnif, Wissem, Mosbah, Amor, and Masmoudi, Ahmed Slaheddine

Subjects
INDOLEACETIC acid, PLANT growth, BACILLUS (Bacteria), SUSTAINABLE chemistry, OPACITY (Optics), RHIZOBACTERIA
Abstract

The detection and quantification of Indole -3 Acetic Acid (IAA) produced by Plant growth promoting rhizobacteria (PGPR) rely on a standard well-documented assay, which remains time-consuming, laborious, and costly. These drawbacks led to sway interest to economic and reliable assays. The aim of this work is to validate and standardize a fast, reliable, and cost-effective microassay to quantify IAA produced by bacteria with an easy microplate method. In order to validate the accuracy of the IAA microplate assay, bacterial samples from different genera were assayed using two methods: the conventional IAA estimation assay and the IAA micro- assay. The microassay shows a prominent reduction in used bacterial supernatant volume as well as Salkowski reagent volume of about 92.5%. It is considerably cheaper than the conventional one of around 56%. The newly performed microplate assay is 23 times faster. The result of IAA quantitative analysis for 13 bacterial strains showed that Bacillus muralis and Bacillus toyonensis produced the highest IAA concentration (23.64±0.003μg/ml and 23.35±0.006μg/ml, respectively). The obtained data from both methods were highly correlated with an R-value of 0.979. The microassay offers the ability to read the optical density of all samples simultaneously since used volumes of bacterial supernatants and Salkowski reagent were minimized to place the mixture in 96-well microplates, which reduces greatly required labor. Furthermore, the application of the IAA micro-plate assay reduces drastically the reagent waste and toxicity hazard of Salkowski reagent in the environment, thus, we can classify it as eco-friendly respecting the Green Chemistry concept according to Environmental Protection Agency (EPA). The IAA microassay is a, reliable, rapid and cost-effective and eco-friendly method to screen plant growth promoting potential of more than 23 bacterial strains by microplate. It could be an alternative for the conventional IAA assay as a routine research tool. [ABSTRACT FROM AUTHOR]

Published
2023
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69. Investigation of the Chemical Composition and Antioxidant and Antimicrobial Activities of Lobularia maritima: Potent Therapeutic Applications

Author

Kouidhi, Soumaya, primary, Zidi, Oumaima, additional, Abdelwahed, Soukaina, additional, Souissi, Yasmine, additional, Trabelsi, Najla, additional, Redissi, Alaeddine, additional, Hamdi, Manel, additional, Trabelsi, Emna, additional, Amara, Yosra, additional, Bhiri, Taher, additional, Khrouf, Rim, additional, Selmi, Boulbaba, additional, Achour, Sami, additional, Cherif, Ameur, additional, Mnif, Wissem, additional, and Mosbah, Amor, additional

Published
2021
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72. Multi-Solvent Extraction Procedure for the Pioneer Fecal Metabolomic Analysis—Identification of Potential Biomarkers in Stable Kidney Transplant Patients

Author

Kouidhi, Soumaya, primary, Souai, Nessrine, additional, Alhujaily, Muhanad, additional, Zidi, Oumaima, additional, Kochbati, Ameni, additional, Redissi, Alaeddine, additional, Belali, Tareg M., additional, Kossai, Imene El, additional, El Manaa, Jamelddine El, additional, Cherif, Ameur, additional, Mnif, Wissem, additional, and Mosbah, Amor, additional

Published
2021
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74. Quantum-mechanical characterization of (ZnO)nnanoclusters and their coronene composites

Author

Yasir, Muhammad, Akram, Fatima, Masih, Mumtaz, Jamil, Nuzhat, Mnif, Wissem, Alkhaloofa, Fawzeyah, Idrees Jubara, Fatima Aldaw, Iqbal, Munawar, and Nazir, Arif

Abstract

Adsorption of zinc oxide Zn8O8(n = 1–8) nanoclusters on coronene was studied through DFT calculations at B3LYP/6-31G (d,p) level. Electronic properties such as HOMO/LUMO energy gap (Eg), ionization potential (IP), electron affinity (EA), chemical hardness and chemical softness were analyzed to understand the sensing abilities of nano-cages for coronene. Egof coronene nano-cages decreased significantly as the zinc oxide ZnnOn(n = 1–8) was adsorbed on the coronene and the Egvalues were recorded to be in the range of 5.75–4.46 eV. It is observed that the alteration of Egduring the adsorption process was transmitted to the sensitivity of the adsorbent for the meticulous adsorbate. The most stable geometry according to the HOMO/LUMO energy gap was found to be Coronene-Zn4O4,while the less stable was Coronene-ZnO. The reactivity indicated the maximum conductance for zinc oxide ZnnOn(n = 1–8) adsorption on coronene. The highest chemical softness as well as electron affinity were recorded for Coronene-ZnO geometry, while the chemical hardness and ionization potential were higher in the case of Coronene-Zn6O6. Zinc oxide adsorption on coronene suggested it to be the best adsorbent for catalysis and magnetism. Such composites of ZnO nanoclusters over coronene exhibited excellent electronic and magnetic properties which suggested their numerous biological, environmental and pharmaceutical applications.

Published
2024
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75. In-vitro antioxidant and anti-inflamatory activities of Pituranthos chloranthus and Artemisia vulgaris from Tunisia

Author

Ben Nasr, Soumeya, AAZZA, Smail, Mnif, Wissem, and Miguel, Maria

Subjects
Radical scavenging ability, Lipid peroxidation prevention, Lipoxygenase, Guezzah, Aljen, Mugwort
Abstract

Pituranthos chloranthus and Artemisia vulgaris L. are two of the most important aromatic and medicinal species from the Apiaceae and Asteraceae families, respectively. They are traditionally used in certain pathologies in which inflammatory processes are involved. The present study investigates the potential of aqueous extracts of Tunisian P. chloranthus and A. vulgaris as a natural alternative source of antioxidant and anti-inflammatory activities using in-vitro techniques. The antioxidant activities of aqueous extracts were evaluated through several assays: capacity for scavenging free radicals (ABTS, DPPH, hydroxyl, superoxide, nitric oxide); and total antioxidant capacity by ferric reducing power activity and inhibition of lipid peroxidation by thiobarbituric acid reactive species (TBARS) method. The anti-inflammatory activity was evaluated through the lipoxygenase (LOX) inhibitory activity. P. chloranthos aqueous extract presented higher concentration of total phenols than A. vulgaris extract, nevertheless higher capacity for scavenging ABTS, superoxide, hydroxyl. and NO free radicals. In the presence of liver homogenate, both extracts had poorer antioxidant activity than in the remaining lipid substrates. P. chloranthos extract had a higher ability for inhibiting lipoxygenase twice higher than A. vulgaris, while it had lower capacity for reducing Fe3+ than P. chloranthos extract. Our results suggest that there are differences of antioxidant activity between both samples, but also the strength for inhibiting the oxidation is highly dependent on the method used. info:eu-repo/semantics/publishedVersion

Published
2020

82. Lobularia maritima leave extract, a nutraceutical agent with antioxidant activity, protects against CCl4-induced liver injury in mice.

Author

Ben Hsouna, Anis, Dhibi, Sabah, Dhifi, Wissal, Ben Saad, Rania, Brini, Faical, Hfaidh, Najla, Almeida, Jackson Roberto Guedes da Silva, and Mnif, Wissem

Subjects
LIVER injuries, CARBON tetrachloride, IRON chelates, IRON ions, BIOMARKERS, OXIDATIVE stress, FERULIC acid, ETHANOL
Abstract

Lobularia maritima (Alyssum maritimum, Brassicaceae), commonly known as sweet alyssum, is an annual ornamental halophyte widely spread along the Tunisian seashore. Lobularia maritima leaf ethanol extract was tested in an experimental model of hepatotoxicity induced by carbon tetrachloride (CCl4). L. maritima extract was found to possess in vitro antioxidant activity by scavenging the DPPH radical (IC50= 45 μg/mL), reducing/chelating iron ions and inhibiting liver lipid peroxidation induced by FeSO4. The levels of total phenolics and flavonoids were 175 ± 2.66 mg GAE/g, and 35 ± 2.88 mg QE/g respectively. Moreover, HPLC analysis revealed six compounds, namely gallic, salicylic, ellagic and ferulic acids as well as catechin and quercetin. A mice model of acute liver injury was successfully established after a single intraperitoneal injection of CCl4, as evidenced by histological analysis, Masson trichrome and Sirius red staining. Compared with the CCl4 intoxicated group, the L. maritima treatment resulted to reduce the liver serum marker enzymes, lipid peroxidation and increased the activities of antioxidant enzymes with further amelioration in the oxidative stress. The present findings discover the therapeutic potentials of L. maritima empowered with promising natural leads for the treatment of oxidative stress associated health disorders by attenuating free radicals, inhibiting lipid peroxidation, and upregulating the tissue-specific antioxidant enzymes. [ABSTRACT FROM AUTHOR]

Published
2022
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83. A new pioneer colorimetric micro-plate method for the estimation of ammonia production by plant growth promoting rhizobacteria (PGPR).

Author

Abdelwahed, Soukaina, trabelsi, Emna, Saadouli, Ilhem, Kouidhi, Soumaya, Masmoudi, Ahmed Slaheddine, Cherif, Ameur, Mnif, Wissem, and Mosbah, Amor

Subjects
PLANT growth, RHIZOBACTERIA, PLANT development, SUSTAINABLE chemistry, BACILLUS (Bacteria), AMMONIA
Abstract

The ability of Plant Growth Promoting Rhizobacteria (PGPR) to produce ammonia (NH3) is one of the key mechanisms for the growth and development of plants. Ammonia spectrophotometric quantification assay using Nessler's reagent remains laborious and seems to be not suitable for large-scale PGP screening assay. Here, we performed a new accurate, easy, and cost-effective micro-plate protocol for large-scale ammonia quantification in bacterial supernatants as an alternative to the spectrophotometric method. In order to validate the accuracy of our innovative microplate assay, 9 bacterial strains were explored for their ability to produce ammonia using both the conventionally described assay and the newly performed micro-plate one. The ammonia 96-well microplate assay was successfully performed by scaling down the spectrophotometric methods to reduce the volume of bacterial supernatant as well as Nessler's reagent. The reduction was estimated to 90%of the total used volume in comparison to the conventional test. The micro-test is 10-fold cheaper and 26 times faster than the conventional method. All bacterial isolates were positive for ammonia production. Bacillus inaquorsum and Bacillus mojavensis produced the highest ammonia concentration of about 371 and 370μM respectively. Furthermore, the application of the ammonia micro-plate assay reduces drastically the reagent waste and toxicity hazard of K2HgI4 (Nessler's reagent) in the environment, thus, we can classify it as eco-friendly respecting the Green Chemistry concept according to Environmental Protection Agency (EPA). The statistical data obtained from both assays are significantly correlated (r = 0.985, R squared = 0.9329, and p < 0.001) proving the accuracy of the micro-plate assay. The proposed NH3 micro-assay is a reliable, rapid, eco-friendly, and cost-effective method to screen plant growth-promoting potential of more than 25 bacterial strains in one micro-plate. It could be an alternative for the conventional NH3 assay as a routine research tool. [ABSTRACT FROM AUTHOR]

Published
2022
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85. Dielectric, ferroelectric, optical and photocatalytic properties of Zn-doped SrFe12-xZnxO19synthesized via a microemulsion route

Author

Bibi, Ismat, Majid, Farzana, Jilani, Kashif, Kamal, Shagufta, Taj, Babar, Nazeer, Zarish, Abbas, Muhammad, Mnif, Wissem, Iqbal, Munawar, Nazir, Arif, and Alwadai, Norah

Abstract

In this study, SrFe12O19and a series of Zn doped SrFe12-xZnxO19nanoparticles (NPs) were synthesized by microemulsion route and the effect of substitution was examined based on morphological, structural, ferroelectric, optical, dielectric as well as photo-catalytic properties. X-ray diffraction pattern reflects the formation of hexagonal ferrite in the 56–73 nm range. The P-E hysteresis loop exhibited increased values of remnant polarization and saturation polarization with higher Zn content. The tangent loss, dielectric loss and dielectric constant values were decreased by increasing frequency while AC conductivity was improved by doping. The photoluminescence spectra exhibited greater charge separation and reduced recombination rate in highly doped material which is ascribed to the improved photocatalytic activity. The optical bandgap was tuned from 2.67 to 1.34eV. Moreover, the photo-catalytic activity of doped and undoped samples was investigated under sunlight irradiation by using crystal violet (CV) dye and 85 % degradation was observed in 70 min vs. pristine SrFe12O19(47 %). The SrFe12-xZnxO19's higher photocatalytic activity under sunlight and narrow bandgap presents them as an encouraging photocatalyst for the elimination of harmful and toxic wastes from industrial wastewater.

Published
2024
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93. The influence of organs on biochemical properties of Tunisian Thuja occidentalis essential oils

Author

Bellili, Sana, Aouadhi, Chedia, Dhifi, Wissal, Ghazghazi, Hanene, Jlassi, Chaima, Sadaka, Carmen, El Beyrouthy, Marc, Maaroufi, Abderrazak, Cherif, Ameur, Mnif, Wissem, Bellili, Sana, Aouadhi, Chedia, Dhifi, Wissal, Ghazghazi, Hanene, Jlassi, Chaima, Sadaka, Carmen, El Beyrouthy, Marc, Maaroufi, Abderrazak, Cherif, Ameur, and Mnif, Wissem

Abstract

In this study, the chemical composition and biological activities of the essential oil (EO) extracts (from leaves and cones) of the Tunisian Thuja occidentalis were evaluated. The composition of the leaf EO extract was more complex than that of the cones. The major components of the leaf EO extract were α-Pinene (34.4%), cedrol (13.17%), and β-Phellandrene (8.04%), while the composition of the cone EO extract was characterized by the predominance of α-Pinene (58.55%) and 3-Carene (24.08%). All EO extracts showed much better antioxidant activity than Trolox against 2, 20-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging, but EOs extracted from leaves exhibited the highest total antioxidant activity. All EOs showed strong antibacterial and antifungal activities against nine tested foodborne microorganisms (Bacillus cereus American Type Culture Collection (ATCC) 1247, Listeria monocytogenes ATCC 7644, Staphylococcus aureus ATCC 29213, Aeromonas hydrophila EI, Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 27853, Salmonella typhimurium NCTC 6017, Aspergillus flavus (foodborne isolate), and Aspergillus niger CTM 10099. The highest antimicrobial activities by disk diffusion assay were recorded for the EOs extracted from leaves, while no difference in potency was marked between leaf and cone EO extracts by the agar dilution method. The most potent antimicrobial activity was recorded among fungi. This study confirms the strong antimicrobial and antioxidant potential of EO extracts from the Tunisian T. occidentalis (from the Sidi Bou Said site), highlighting its potential as a natural preservative against foodborne pathogens, particularly against E. coli and S. typhimurium.

Published
2018

99. Secondary metabolism responses in two Pisum sativum L. cultivars cultivated under Fe deficiency conditions

Author

Jelali, Nahida, Moez, Smiri, Dhifi, Wissal, Mnif, Wissem, Abdelly, Chedly, and Gharsalli, Mohamed

Subjects
Iron deficiency, bicarbonate, phenolic metabolism, antioxidative enzymes, Pisum sativum
Abstract

The present study was carried out to investigate the Fe deficiency effect on the secondary metabolism responses in two Pisum sativum cultivars characterized by different tolerance to Fe deficiency. Previous study investigating the physiological responses to Fe deficiency in these two pea cultivars showed that Kelvedon was more tolerant than Lincoln. Both cultivars were grown in the absence or presence of Fe with the addition of bicarbonate for twelve days. Higher concentrations of phenols and flavonoids were observed in Fe-deficient tissues of both cultivars; however, the increase was greater in the tolerant cultivar than in the susceptible one. The activity of shikimate pathway enzymes tested was more enhanced in the tolerant cultivar. In addition, lipid peroxidation and H2O2 concentrations were more increased in the susceptible cultivar when compared with the tolerant one. Peroxidase activity was increased in the tolerant cultivar grown under bicarbonate supply, while a considerable diminution was observed in the susceptible one, suggesting the involvement of this antioxidant enzyme in the tolerance of pea to Fe deficiency. The lignifying peroxidases activity was more decreased in Lincoln than in Kelvedon, especially in the presence of bicarbonate. Our data suggest that the tolerance of Kelvedon was related to its ability to modulate the phenolic metabolism pathway and to enhance the antioxidant potentials.Key words: Iron deficiency, bicarbonate, phenolic metabolism, antioxidative enzymes, Pisum sativum.

Published
2016

100. Phytochemical identification of volatile fraction, essential oil and screening of antioxidant, antibacterial, allelopathic and insecticidal potential from Artemisia herba-alba leaves

Author

Bellili, Sana, primary, Jazi, Sabrine, additional, Hrira, Mohamed Yahya, additional, Lamari, Ali, additional, Dhifi, Wissal, additional, Diouani, Mohamed Fethi, additional, Araújo, Maria Eduarda, additional, Cioni, Pier Luigi, additional, Flamini, Guido, additional, Cherif, Ameur, additional, and Mnif, Wissem, additional

Published
2017
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