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The aim of this study was to investigate if the biomarkers myelin basic protein (MBP) and neurofilament-H (NF-H) yielded informative value in forensic diagnostics when examining cadaveric cerebrospinal fluid (CSF) biochemically via an enzyme-linked immunosorbent assay (ELISA) and comparing the corresponding brain tissue in fatal traumatic brain injury (TBI) autopsy cases by immunocytochemistry versus immunohistochemistry. In 21 trauma and 19 control cases, CSF was collected semi-sterile after suboccipital puncture and brain specimens after preparation. The CSF MBP (p = 0.006) and NF-H (p = 0.0002) levels after TBI were significantly higher than those in cardiovascular controls. Immunohistochemical staining against MBP and against NF-H was performed on cortical and subcortical samples from also biochemically investigated cases (5 TBI cases/5 controls). Compared to the controls, the TBI cases showed a visually reduced staining reaction against MBP or repeatedly ruptured neurofilaments against NF-H. Immunocytochemical tests showed MBP-positive phagocytizing macrophages in CSF with a survival time of > 24 h. In addition, numerous TMEM119-positive microglia could be detected with different degrees of staining intensity in the CSF of trauma cases. As a result, we were able to document that elevated levels of MBP and NF-H in the CSF should be considered as usefulneuroinjurybiomarkers of traumatic brain injury.

In forensic science determination of the origin and sex of skeletal remains is an important task for identification purposes. In this study we investigated the krotaphion-sphenion distance (K‑S distance) in the pterion region of German, Euro-American, African-American and Rwandan skulls of modern individuals from the nineteenth to the twenty-first century to look for statistically significant differences in sex and ancestry. We found a statistically significant sex-specific difference in the K‑S distance, which was greater in male skulls than in female skulls for both sides of the skull. Our study also showed that there is a statistically significant difference in the K‑S distance between the four populations studied. Landmarks and morphometric parameters measured in our investigations, which were not used for the present examination were provided to the software program Fordisc for its reference data to enhance the range of its usability for identification of unknown skulls or partial skulls of European individuals., Bei der forensischen Begutachtung zur Identifizierung unbekannter Skelettfunde spielen Herkunftsanlaysen und Geschlechtsbestimmungen eine bedeutende Rolle. In unserer Studie an euroamerikanischen, afroamerikanischen, ruandischen und deutschen Schädeln untersuchte unsere Arbeitsgruppe die sog. Krotaphion-Sphenion-Distanz in der Pterion Region am menschlichen Schädel, um geschlechts- und herkunftsspezifische Unterschiede näher zu beleuchten. Unsere Ergebnisse zeigen einen signifikanten Unterschied in der K‑S-Distanz: Männliche Individuen zeigten auf beiden Seiten des Schädels signifikant größere Werte als weibliche Individuen, des Weiteren waren signifikante Unterschiede unter den vier untersuchten Populationen festzustellen. Die weiteren, im Rahmen der Studie gemessenen, jedoch für die vorliegende Auswertung nicht verwendeten Landmarken und morphometrischen Parameter der Schädel gingen in die Datenbank für die Identifizierungs-Software Fordisc ein, um deren Datengrundlage und damit Nutzbarkeit zur Identifikation unbekannter Schädel oder Schädelteile europäischer Individuen zu verbessern.

The aim of the present study was a refined analysis of neuroinflammation including TMEM119 as a useful microglia-specific marker in forensic assessments of traumatic causes of death, e.g., traumatic brain injury (TBI). Human brain tissue samples were obtained from autopsies and divided into cases with lethal TBI (n = 25) and subdivided into three groups according to their trauma survival time and compared with an age-, gender-, and postmortem interval-matched cohort of sudden cardiovascular fatalities as controls (n = 23). Brain tissue samples next to cortex contusions and surrounding white matter as well as samples of the ipsilateral uninjured brain stem and cerebellum were collected and stained immunohistochemically with antibodies against TMEM119, CD206, and CCR2. We could document the highest number of TMEM119-positive cells in acute TBI death with highly significant differences to the control numbers. CCR2-positive monocytes showed a significantly higher cell count in the cortex samples of TBI cases than in the controls with an increasing number of immunopositive cells over time. The number of CD206-positive M2 microglial cells increased survival time-dependent. After 3 days of survival, the cell number increased significantly in all four regions investigated compared with controls. In sum, we validate a specific and robustly expressed as well as fast reacting microglia marker, TMEM119, which distinguishes microglia from resident and infiltrating macrophages and thus offers a great potential for the estimation of the minimum survival time after TBI. Electronic supplementary material The online version of this article (10.1007/s00414-020-02384-z) contains supplementary material, which is available to authorized users.

Intrakranielle Blutungen sind im Säuglingsalter seltene, aber lebensbedrohende Ereignisse. Neben Gefäßmissbildungen, Stoffwechseldefekten sowie Störungen der Blutgerinnung kommen v. a. nichtakzidentielle Traumata, Schütteltrauma in Betracht. Die klinische Diagnostik umfasst hinsichtlich der Blutungsgenese neben Sonographie und MRT als apparatives Verfahren auch eine Fundoskopie sowie laborchemische Analysen, insbesondere der Gerinnungsparameter. Für die Blutgerinnung ist das fettlösliche Vitamin K essenziell: Frühe, klassische und späte Vitamin-K-Mangel-Blutungen werden dabei unterschieden. Um ein gehäuftes Wiederauftreten von Vitamin-K-Mangel-Blutungen bei Neugeborenen und jungen Säuglingen zu verhindern, bedarf es einer hinreichenden Aufklärung der Eltern. Eine Verweigerung der Prophylaxe scheint Folge einer weltanschaulich begründeten Ablehnung der Schulmedizin und ein zunehmendes Phänomen in wohlhabenden Industrieländern zu sein., Intracranial hemorrhages in infants are rare but life-threatening events. Apart from vascular malformations, metabolic disorders and coagulopathies, nonaccidental trauma, in particular shaken baby syndrome must be taken into consideration. Clinical diagnostic tests and procedures to further evaluate the etiology of the hemorrhage include sonography and magnetic resonance imaging (MRI) as imaging procedures as well as fundoscopy and laboratory tests, especially with respect to coagulation parameters. Fat-soluble vitamin K is essential for blood coagulation. A differentiation is made between classical and delayed hemorrhages due to vitamin K deficiency. In order to avoid an increased recurrence of bleeding due to vitamin K deficiency in neonates and young infants, an adequate clarification for the parents is necessary. A refusal of prophylaxis seems to be the result of an ideologically founded rejection of classical medicine and an increasing phenomenon in affluent industrial countries.

Michael Bohnert führt in diesem Buch leicht verständlich in die Rechtsmedizin ein. Er erklärt die verschiedenen Untersuchungsmethoden sowie Wissenswertes zur Thanatologie und medizinischen Kriminalistik. Ausführlich beschreibt er die unterschiedlichen Verletzungsarten und ihre Merkmale. Ein Kapitel zu den forensischen Wissenschaften rundet diese Einführung ab. Auch für Nichtmediziner geeignet!

The aim of this pilot study was to investigate the diagnostic potential of TMEM119 as a useful microglia-specific marker in combination with immunostainings for phagocytic function and infiltrating capacity of monocytes in cases of lethal monosubstance intoxications by morphine (MOR), methamphetamine (METH), and of ethanol-associated death (ETH) respectively. Human brain tissue samples were obtained from forensic autopsies of cases with single substance abuse (MOR, n = 8; ETH, n = 10; METH, n = 9) and then compared to a cohort of cardiovascular fatalities as controls (n = 9). Brain tissue samples of cortex, white matter, and hippocampus were collected and stained immunohistochemically with antibodies against TMEM119, CD68KiM1P, and CCR2. We could document the lowest density of TMEM119-positive cells in MOR deaths with highly significant differences to the control densities in all three regions investigated. In ETH and METH deaths, the expression of TMEM119 was comparable to cell densities in controls. The results indicate that the immunoreaction in brain tissue is different in these groups depending on the drug type used for abuse.

Broadband Wireless Access (BWA) based on the IEEE 802.16 standards [IEEE 802.16, 2004] [IEEE 802.16, 2005], also known as Worldwide Interoperability for Microwave Access (WiMAX), is gaining momentum as more and more field trials are transformed in commercial roll-outs. Very much likely, this is certainly a merit of the combined effort of the IEEE 802.16 standardization community, the WiMAX Forum [WiMAX Forum a, 2008] [WiMAX Forum b, 2008] and the research community. As of today, standards have matured, the WiMAX Forum has setup its certification program in order to foster interoperability, and the research community went through technological details up to an extent, such that many operators consider the risks associated with commercial deployment predictable. Yet, WiMAX is still not deemed as globally established. This technology is very new and a competing system, namely Long-Term Evolution (LTE) [3GPP, 2009], is progressing in a similar pace. Which technology will finally make it through in the market, or will we have even several co-existing ones, is, among others, influenced by the research community. The better a technology is understood, the more likely it will be adopted by manufacturers, vendors and operators. Key to scientific consideration is the availability of reliable simulation tools. This applies equally to WiMAX and any other technology and is embodied by an emerging business of commercial simulators like OPNET [OPNET] and Qualcomm [Qualcomm]. Unfortunately, these simulators are fairly expensive and/or their use is strictly licensed. As monetary considerations and openness frequently prevail, in particular in academia, NS-2 [NS-2], as open-source alternative, still retains its position. Naturally, open-source software rarely approaches commercial standards, especially in terms of completeness and documentation. This is, for example, the case for the public available WiMAX module for NS-2. This module supports several WiMAX features but lacks a very essential one, Quality of Service (QoS) support. The lack of this feature 34

Due to its protected anatomical location, cerebrospinal fluid (CSF) is a very stable fluid which undergoes comparatively little change in the early post-mortem phase. While many immunohistochemical markers already established for clinical diagnostic issues in tissue samples obtained by biopsy could meanwhile be translated also to post-mortem tissue, no systematic immunocytochemical investigations have generally been conducted on post-mortem body fluids and for CSF specifically, have not been established at all. CSF as the fluid directly surrounding the brain should also be examined to allow a more detailed characterization of processes in the central nervous system. Comparing traumatized tissue and CSF can complete forensic assessment and complement neuropathological evaluation.

Blood-brain barrier (BBB) integrity is one of the important elements of central nervous system (CNS) homeostasis. MicroRNAs (miRs) have been demonstrated to play a role in many CNS disorders such as stroke and traumatic brain injury. MiR-212/132 are highly expressed in the CNS but their role at the BBB has not been characterized yet. Thus, we analyzed the expression of miR-212/132 in hypoxic mouse and human brain microvascular endothelial cells (BMEC) as well as in posttraumatic mouse and human brain tissue and serum exosomes. MiR-212/132 expression was detected in brain capillaries by in situ hybridization and was increased up to ten times in hypoxic BMEC. Over-expression of pre-miR-212/132 in BMEC decreased barrier properties and reduced migration of BMEC in the wound healing assay. We identified and validated tight junction proteins claudin-1 (Cldn1), junctional adhesion molecule 3 (Jam3), and tight junction-associated protein 1 (Tjap1) as potential miR-212/132 targets. Over-expression of miRs led to a decrease in mRNA and protein expression of Cldn1, Jam3, and Tjap1, which could be rescued by a respective anti-miR. In conclusion, our study identifies miR-212/132 as critical players at the hypoxic BBB. In addition, we propose three new direct miR-212/132 targets to be involved in miR-212/132-mediated effects on BBB properties. Electronic supplementary material The online version of this article (10.1007/s12975-018-0683-2) contains supplementary material, which is available to authorized users.

Die medizinische Versorgung verletzter Personen hat Vorrang vor der forensischen Befunddokumentation. Das eine schliest das andere jedoch nicht aus und die Dokumentation von Verletzungen kann durchaus in die Wundversorgung integriert werden. Wesentlich ist vor allem, dass man sich daruber im Klaren ist, dass nicht nur der klinische, sondern auch der forensische Blick auf eine verletzte Person wichtig ist.

Wahrend der praktischen Arbeit im Rettungsdienst auf der einen Seite und der arztlichen Tatigkeit in der Rechtsmedizin auf der anderen Seite wird immer wieder deutlich, dass es in der Notfallmedizin weit verbreitet ist, nichts oder nur wenig uber die Arbeit der Rechtsmedizin zu wissen. Jeder kennt zwar die einschlagigen Fernsehserien und in nicht wenigen Kinofilmen schubst der Rechtsmediziner den Notarzt an der Einsatzstelle quasi zur Seite – mit dem wahren Leben hat dies aber wohl nichts zu tun. Es gibt in der Realitat allerdings erstaunlich viele Beruhrungspunkte der beiden Fachgebiete, von denen man im Rettungsdienst nur selten etwas mitbekommt, da in der Praxis zumeist die einzelnen Bereiche nacheinander zum Einsatz kommen, ohne sich personlich zu begegnen.

Sie werden als Notarzt zu einem Verkehrsunfall gerufen, bei dem ein Fusganger von einem Pkw angefahren wurde. Der Fusganger hat neben Hamatomen und Schurfungen eine Fraktur des Unterschenkels erlitten. Nachdem Sie sich um ihn gekummert haben, werden Sie von der Polizei gebeten, sich auch noch den Pkw-Fahrer anzuschauen.

Several relevant research and innovation activities have recently investigated the technical and economic advantages of cloud computing for the provisioning of telco service infrastructures, in particular towards all-IP next generation 5G networks. In fact, the evolution of telco service infrastructures traditionally requires a significant upfront investment (and a long adoption process). Conversely, cloud exploitation significantly lowers investment risks by potentially providing elasticity in service provisioning via flexible Virtual Network Functions (VNFs) on top of a Network Functions Virtualization (NFV) Infrastructure. In this context, the paper presents novel solutions that we have designed, implemented, and evaluated within the EU FP7 Mobile Cloud Networking project (MCN). Their aim is to achieve cost-effective elastic provisioning of telco services over heterogeneous and federated cloud providers, with the specific focus of supporting the extreme quality levels that are demanded by traditional, non-virtualized, and dedicated telco infrastructures. In particular, we concentrate on how to effectively and efficiently automate service state migration for coarse-grained telco service (cloudified) components by leveraging industry-mature orchestration technologies and cloud management frameworks. While our proposed state migration model and procedure are general, its implementation is experimented for MCN's Rating, Charging, and Billing as a Service (RCBaaS). This MCN functionality has been chosen by purpose due to its challenging reliability and uptime requirements. The reported experimental and simulation results show the technical feasibility of the proposed solution under different and realistic load conditions for next-generation and cloudified 5G services.

An einem heisen Augustnachmittag werden Sie unter dem Stichwort „Bewusstlose Person“ in eine burgerliche Wohngegend gerufen. An der genannten Adresse angekommen, werden Sie durch einen Nachbarn empfangen, der angibt, seine allein lebende 84-jahrige Nachbarin leblos in der Wohnung aufgefunden zu haben. Er habe sich mittels des bei ihm fur Notfalle deponierten Schlussels zur Wohnung Zutritt verschafft, nachdem die Nachbarin bei horbarer Musik aus der Wohnung auf Klingeln nicht offnete.

In the last few years, quantitative analysis of metabolites in body fluids using LC/MS has become an established method in laboratory medicine and toxicology. By preparing metabolite profiles in biological specimens, we are able to understand pathophysiological mechanisms at the biochemical and thus the functional level. An innovative investigative method, which has not yet been used widely in the forensic context, is to use the clinical application of metabolomics. In a metabolomic analysis of 41 samples of postmortem cerebrospinal fluid (CSF) samples divided into cohorts of four different causes of death, namely, cardiovascular fatalities, isoIated torso trauma, traumatic brain injury, and multi-organ failure, we were able to identify relevant differences in the metabolite profile between these individual groups. According to this preliminary assessment, we assume that information on biochemical processes is not gained by differences in the concentration of individual metabolites in CSF, but by a combination of differently distributed metabolites forming the perspective of a new generation of biomarkers for diagnosing (fatal) TBI and associated neuropathological changes in the CNS using CSF samples.

In this casuistry, two accidents from Germany and Switzerland are presented that happened during the shot of recoilless anti-tank weapons. In both cases, the injuries led to the death of two soldiers: A 22-year-old soldier in Germany was struck by the counter mass of a so-called Davis gun which had been fired by a comrade during a firing exercise; he died from his severe injuries, especially in the abdominal part of the body. As a peculiarity of the wound morphology, it was found to be a thick-layered, metallic, gray material in the wound cavity, which corresponded to the material of the counter mass that was ejected opposite to the shooting direction. The other case took place in Switzerland, where a 24-year-old soldier was seriously injured during an exercise with portable anti-tank rockets. At the time the shot was fired, he stood behind the launcher and was hit by the propulsion jet of the rocket motor. He died as well from his severe injuries, which were located at the chest done by the gas jet and by the very high pressure. In both cases, two different causes of death were present: massive blunt violence in the first case versus a jet of hot gases of very high speed and temperature in the second case.

During the investigative process that typically follows a criminal act, it may prove necessary to work with and analyze evidence that is not recent but old. This could become necessary, for example, when a crime is discovered some time after it was committed or when a cold case is reopened. Due to this need, the present study focused on the detection and visualization of 2-year-old biological traces. To do so, an alternative light source and different filters were used. The optical behavior of 2-year-old samples of blood, semen, urine, saliva, and sweat located on 19 different materials was documented, analyzed, and compared with the optical behavior of the same samples when they were recent [1].

Background Human hippocampal area Cornu Ammonis (CA) 1 is one of the first fields in the human telencephalon showing Alzheimer disease (AD)-specific neuropathological changes. In contrast, CA2 and CA3 are far later affected pointing to functional differences, which may be accompanied by differences in proteome endowment and changes. Methods Human pyramidal cell layers of hippocampal areas CA1, CA2, and CA3 from neurologically unaffected individuals were excised using laser microdissection. The proteome of each individual sample was analyzed and differentially abundant proteins were validated by immuno-histochemistry. Results Comparison of CA1 to CA2 revealed 223, CA1 to CA3 197 proteins with differential abundance, among them we found motor proteins MYO5A and DYNC1H1. Extension of the study to human hippocampus slices from AD patients revealed extensive depletion of these proteins in CA1 area compared to unaffected controls. Conclusion High abundance of motor proteins in pyramidal cell layers CA1 compared to CA2 and CA3 points the specific vulnerability of this hippocampal area to transport-associated changes based on microtubule dysfunction and destabilization in AD.

Injuries caused by blunt force are seen frequently in daily forensic casework. Sometimes, especially when there is less information about the surrounding circumstances, it might become difficult to figure out the cause and background of injuries: accident, criminal violence or self-infliction? In the study presented, face injuries caused by blunt force in 694 cases were analyzed comparing the injury patterns in accidents to those in crimes. It turned out injuries of the ear and retroauricular region clearly indicate a crime. Also, soft tissue injuries of nose, upper jaw, and lower jaw point towards a criminal violence, whereas tooth injuries occur with a similar frequency in both crimes and accidents.

Cloud computing can greatly enhance robotic applications, not only by complementing robotic resources (e.g., CPU/GPU, memory, storage), but also by providing global-scale highly available services, distributed application management solutions, proven development and deployment practices. However, several challenges have to be addressed in order to bridge the gap between cloud and robotic development, including ROS and cloud design assumptions, networking, and mobile robots. In this chapter we present ECRP, a Platform as a Service solution for building ROS-based cloud robotics applications.

Distributed cloud storage solutions are currently gaining high momentum in industry and academia. The enterprise data volume growth and the recent tendency to move as much as possible data to the cloud is strongly stimulating the storage market growth. In this context, and as a main requirement for cloud native applications, it is of utmost importance to guarantee resilience of the deployed applications and the infrastructure. Indeed, with failures frequently occurring, a storage system should quickly recover to guarantee service availability. In this paper, we focus on containerized cloud storage, proposing a resilience monitoring solution for the recently developed Rook storage operator. While, Rook brings storage systems into a cloud-native container platform, in this paper we design an additional module to monitor and evaluate the resilience of the Rook-based system. Our proposed module is validated in a production environment, with software components generating a constant load and a controlled removal of system elements to evaluate the self-healing capability of the storage system. Failure recovery time revealed to be 41 and 142 seconds on average for a 32GB and a 215GB object storage device respectively.

This paper states the research done in building a Wildfire Propagation Simulation tool able to present graphically, how the fire will be propagated in case of a wildfire. The core of this tool was developed using Cellular Automata (CA) relayed on mathematical foundations for modeling the propagation and the transition rules of the CA. Also, the mathematical model was combined with a geographical information system provided by Google Maps API, allowing to conduct a simulation that considers the kind and density of vegetation in a specific zone of interest. Finally, we define and perform a full simulation providing information for managing and predicting wildfires and, the future works derived from this research.

The concept of spontaneous combustion has huge appeal as an historical fiction but it has no scientific background. However, in some deaths involving fire, a body can burn away virtually completely with minimal thermal destruction to the neighboring environment. We report an extraordinary case of the self-immolation of an elderly woman who set herself on fire with suicidal intentions. The unusual appearance and location of the body closely resembled the phenomenon of so-called spontaneous human combustion because the upper parts of the body were almost totally destroyed by fire, while the legs and surrounding structures remained almost untouched by flames. The results of all investigations proved that the woman set fire to her body using a box of matches and accelerants (concentrated ethanol and a solid firelighter). Near-total combustion of her body subsequently occurred during the postmortem period. The development of alleged spontaneous human combustion requires the following: ignition (external heat source), fuel (molten human fat), a wick (e.g. charred and porous clothing, bedding, or ground), time, and an optimal microclimate for gradual burning.

Crania are a reliable source for sex estimation in Euro-Americans, Europeans, and most other populations. Besides morphological assessments, the application of Fordisc® has become a useful tool within the last two decades, creating discriminant functions from morphometric data. Unfortunately, until now, white populations are mostly represented by measurements of American individuals. Therefore, classification rates are lower for European skulls than for Euro-Americans. The aim of this study was to show differences in sexual dimorphism between German and Euro-American crania. Furthermore, their secular change from the nineteenth to the twentieth century has been investigated. Analyses have been performed on glabella subtense (GLS), mastoid height (MDH), and bizygomatic breadth (ZYB). Fordisc® 3.1 was used to study sexual dimorphism and secular change, whereas SAS® was used to perform a two-level ANOVA to test for variation in sex dimorphism. Euro-Americans show greater dimorphism than Germans in all three measurements tested. This larger difference is even increasing from the late nineteenth through the late twentieth century in terms of GLS and MDH, while it stays almost the same in the present Europeans. These results explain the unsatisfying classification rates of German and other European crania on Fordisc®. Data collection for European Fordisc® samples is in progress and should improve the current situation.

In medico-legal literature, only a small number of publications deal with lethal injuries caused by shots with modified guns. This might lead to the conclusion that such cases are extremely rare. However, there are cases again and yet again. During the investigation process, the modified gun is of particular importance since it can show an unusual ballistic behaviour. The present paper reports on a suicide of a 60-year-old man, committed with a modified revolver and a lead bullet. The man had a single gunshot wound with entrance at the right temporal bone. Autopsy revealed that the bullet had fragmented into two major parts. The smaller one stood outside the cranial cavity and pushed its way alongside between the cranial bone and scalp to its end position in the left temporal area. The bigger part entered the cranial cavity and ended in the left parietal lobe. In shots on ballistic soap and on a head-model, the ballistics of the weapon and lead bullet were characterized. The angle necessary for bullet fragmentation was determined by shots on ballistic soap and turned out to be 55°-60° at a velocity of around 200 m/s. This knowledge was transferred to contact shots on a head-model consisting of a layered polyurethane sphere filled with 10 % ballistic gelatine and covered with a skin-like cap almost all around. The resulting injury pattern corresponded to the one of the suicide person. The bigger bullet part entered the skull while the smaller part pushed its way alongside between skin and skull causing an outer contour shot. Furthermore, the revolver was documented firing off two bullets by one trigger pull-a phenomenon of importance for forensic casework the authors have not found reported in forensic literature.