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760 results on '"Masuda, Shinji"'

55. Bacterial Pathogen Infection Triggers Magic Spot Nucleotide Signaling in Arabidopsis thalianaChloroplasts through Specific RelA/SpoT Homologues

Author

Qiu, Danye, Lange, Esther, Haas, Thomas M., Prucker, Isabel, Masuda, Shinji, Wang, Yan L., Felix, Georg, Schaaf, Gabriel, and Jessen, Henning J.

Abstract

Magic spot nucleotides (p)ppGpp are important signaling molecules in bacteria and plants. In the latter, RelA-SpoT homologue (RSH) enzymes are responsible for (p)ppGpp turnover. Profiling of (p)ppGpp is more difficult in plants than in bacteria due to lower concentrations and more severe matrix effects. Here, we report that capillary electrophoresis mass spectrometry (CE-MS) can be deployed to study (p)ppGpp abundance and identity in Arabidopsis thaliana. This goal is achieved by combining a titanium dioxide extraction protocol and pre-spiking with chemically synthesized stable isotope-labeled internal reference compounds. The high sensitivity and separation efficiency of CE-MS enables monitoring of changes in (p)ppGpp levels in A. thalianaupon infection with the pathogen Pseudomonas syringaepv. tomato (PstDC3000). We observed a significant increase of ppGpp post infection that is also stimulated by the flagellin peptide flg22 only. This increase depends on functional flg22 receptor FLS2 and its interacting kinase BAK1 indicating that pathogen-associated molecular pattern (PAMP) receptor-mediated signaling controls ppGpp levels. Transcript analyses showed an upregulation of RSH2upon flg22 treatment and both RSH2and RSH3after PstDC3000infection. Arabidopsismutants deficient in RSH2 and RSH3 activity display no ppGpp accumulation upon infection and flg22 treatment, supporting the involvement of these synthases in PAMP-triggered innate immune responses to pathogens within the chloroplast.

Published
2023
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72. Jamestown Canyon virus detection in human tissue specimens

Author

Zheng, Huachuan, Murai, Yoshihiro, Hong, Mei, Nakanishi, Yuko, Nomoto, Kazuhiro, Masuda, Shinji, Tsuneyama, Koichi, and Takano, Yasuo

Subjects
Arbovirus diseases -- Diagnosis, Arbovirus diseases -- Research, Tissue microarrays -- Research, Tissue microarrays -- Usage, Health
Published
2007

76. Light induced structural changes of a full-length protein and its BLUF domain in YcgF(Blrp), a blue-light sensing protein that uses FAD (BLUF)

Author

Hasegawa, Koji, Masuda, Shinji, and Ono, Taka-aki

Subjects
Fourier transform infrared spectroscopy -- Usage, Bacterial proteins -- Chemical properties, Photoreceptors -- Research, Biological sciences, Chemistry
Abstract

Light-induced structural changes for the signaling state formation are studied using the light-induced Fourier transform infrared (FTIR) difference spectroscopy of both the full-length YcgF protein (YcgF-Full) and its BLUF domain (YcgF-BLUF). YcgF-Full and YcgF-BLUF show identical UV-visible absorption redshifts for FAD upon illumination, and the possibility of full-length-specific protein bands being responsible for the C-terminal EAL domain structural changes in the signaling state due to light excitation of the N-terminal BLUF domain is discussed.

Published
2006

78. Adenylyl cyclase activity of cya1 from the cyanobacterium Synechocystis sp. strain PCC 6803 is inhibited

Author

Masuda, Shinji and Ono, Taka-aki

Subjects
Cyanobacteria -- Genetic aspects, Adenylate cyclase -- Research, Genetic research, Biological sciences
Abstract

Bicarbonate stimulates the activities of several class III adenylyl cyclases studied to date. However, we show here that bicarbonate decreased [V.sub.max] and substrate affinity in Cya1, a major adenylyl cyclase in the cyanobacterium Synechocystis sp. strain PCC 6803. This indicates that manifestation of the bicarbonate responsiveness is specifically modulated in Cya1.

Published
2005

79. Structure of a novel photoreceptor, the BLUF domain of APPA from Rhodobacter sphaeroides

Author

Aderson, Spencer, Dragnea, Vladimira, Masuda, Shinji, Joel Ybe, Moffat, Keith, and Bauer, Carl

Subjects
Biochemistry -- Chemical properties, Protein binding -- Research, Photoreceptors -- Research, Photoreceptors -- Structure, Biological sciences, Chemistry
Abstract

The three-dimensional dark state structure of the flavin-binding BLUF (blue light sensing using FAD) domain of AppA from Rhodobacter sphaeroides is described. The domain represents a new class of flavin-binding proteins involved in light sensing, its structure to that of other related proteins is compared and the implications of the structure for signaling mechanisms is discussed.

Published
2005

82. Null mutation of HvrA compensates for loss of an essential relA/spoT-like gene in Rhodobacter capsulatus

Author

Masuda, Shinji and Bauer, Carl E.

Subjects
Biological sciences
Abstract

We report that a single relA/spoT-like gene exists on the Rhodobacter capsulatus chromosome, and its mutational loss is lethal This gene could he mutated only under a mutational background of a null mutation in the nucleoid protein HvrA. This result suggests that there may be a direct link between HvrA-regulated promoters and the ppGpp-related stringent response.

Published
2004

85. Mutational analyses of the photosynthetic reaction center-bound triheme cytochrome subunit and cytochrome c(sub)2 in the purple bacterium Rhodovulum sulfidophilum

Author

Masuda, Shinji, Tsukatani, Yusuke, Kimura, Yasuaki, Nagashima, Kenji V.P., Shimada, Keizo, and Matsuura, Katsumi

Subjects
Photosynthesis -- Research, Cytochromes -- Physiological aspects, Electron transport -- Physiological aspects, Bacteria, Photosynthetic -- Physiological aspects, Biological sciences, Chemistry
Abstract

Research indicates that in Rhodovulum sulfidophilum cytochrome c(sub)2 is not the dominant electron donor to reaction center as a soluble cytochrome, c-549, donates electron to the reaction center-bound triheme cytochrome. This process is different from those of other purple bacteria.

Published
2002

86. AppA is a blue light photoreceptor that antirepress photosynthesis gene expression in Rhodobacter sphaeroides

Author

Masuda, Shinji and Bauer, Carl E.

Subjects
Photosynthesis -- Regulation, Gene expression -- Physiological aspects, Photoreceptors -- Physiological aspects, DNA binding proteins -- Physiological aspects, Biological sciences
Abstract

The flavin-containing antirepressor AppA functions as a trancription factor and regulates PpsR repressor's binding to DNA by inhibiting the protein binding by either inducing a disulfide bond in PpsR or forming a stable AppA-PpsR(sub)2 antirepressor-repressor complex. Further, the flavin mediated and blue-light induced red shifted photocycle inhibits the complex formation.

Published
2002

92. Transcriptional control of expression of genes for photosynthetic reaction center and light-harvesting proteins in the purple bacterium Rhodovulum sulfidophilum

Author

Masuda, Shinji, Nagashima, Kenji V.P., Shimada, Keizo, and Matsuura, Katsumi

Subjects
Genetic regulation -- Analysis, Bacteria, Photosynthetic -- Research, Oxygen -- Physiological transport, Photosynthesis research -- Genetic aspects, Biological sciences
Abstract

Results demonstrate that the insensitivity to oxygen of Rhodovulum sulfidophilum photosynthetic genes allows the bacteria to actively synthesize photosynthetic apparatus under high aeration. Data reveal the oxygen insensitivity is due to a weak repression of the photosynthetic operon by oxygen.

Published
2000

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