100 results on '"Martinez-Garcia E"'
Search Results
52. First record of the sipunculan worm Phascolion (Phascolion) caupo Hendrix, 1975 in the Mediterranean Sea
- Author
-
FERRERO-VICENTE, L.M., primary, LOYA-FERNANDEZ, A., additional, MARCO-MENDEZ, C., additional, MARTINEZ-GARCIA, E., additional, SAIZ-SALINAS, J.I., additional, and SANCHEZ-LIZASO, J.L., additional
- Published
- 2012
- Full Text
- View/download PDF
53. WHSC1 (Wolf-Hirschhorn syndrome candidate 1)
- Author
-
Martinez-Garcia, E, primary and Licht, JD, additional
- Published
- 2011
- Full Text
- View/download PDF
54. Multi-robot communication architecture for human guiding
- Author
-
Martinez-Garcia, E A, primary, Yoshida, T, additional, Ohya, A, additional, and Yuta, S, additional
- Published
- 2005
- Full Text
- View/download PDF
55. Lámpara de Wood en porfiria eritropoyética congénita
- Author
-
Blasco Morente, G., Martínez Peinado, C., Martínez García, E., and Tercedor Sánchez, J.
- Published
- 2014
- Full Text
- View/download PDF
56. Trajectory control for groups of humans by deploying a team of mobile robots.
- Author
-
Martinez-Garcia, E., Akihisa, O., and Yuta, S.
- Published
- 2005
- Full Text
- View/download PDF
57. Laringoscopia exploradora e intubación traqueal mediante Airtraq® en una paciente pediátrica con síndrome branquio-oto-renal
- Author
-
Martínez García, E., Velasco Martínez, M., Pato Rodríguez, M.D., and Macías Rogado, M.
- Published
- 2013
- Full Text
- View/download PDF
58. Comentarios al artículo “uso de guía metálica para intubación traqueal mediante fibroscopia en un neonato con síndrome de treacher collins y atresia de coanas”
- Author
-
Martínez García, E. and Pérez Gallardo, A.
- Published
- 2010
- Full Text
- View/download PDF
59. Tectonic and Metallogenic Significance of Sedimentary Manganese Deposits in the Eastern Cantabrian Domain, Asturias, Northwestern Spain
- Author
-
Martinez-Garcia, E., Antona, J. F., Sanchez, A. Garcia, and de la Vega, J. L. Quiroga
- Abstract
The eastern part of the Cantabrian Zone (Iberian Massif, Spain) is well known by the variety of small and medium-sized mineral occurrences of Pb-Zn-Ba-Hg, F-Ba, Cu-Co-Ni, Hg-Ba, Sb-As, Fe-Mn, etc., which have been exploited since Neolithic time. There is also a conspicuous Carboniferous Mn-Fe sedimentary deposit consisting either of small nodules in a pelitic matrix or of massive Mn (Ricacabiello Formation of Bashkirian age). Twenty-four samples of nodules and matrix and 2 samples of hydrothermal Fe-Mn have been analyzed, and trace and REE elements have shown the existence of hydrothermal and hydrogenous nodules in the formation, which was deposited in a deep-marine environment related to Late Paleozoic tectonic episodes, and also the possibility of this being the source bed for most of the rest of the epithermal deposits in the Cantabrian Zone during an intensive epoch of hydrothermal circulation in Permian time. more...
- Published
- 2004
- Full Text
- View/download PDF
60. FSMP-07. CYSTATHIONINE-Γ-LYASE DRIVES ANTIOXIDANT DEFENSE IN CYSTEINE-RESTRICTED IDH1 MUTANT ASTROCYTOMAS
- Author
-
Cano-Galiano A, Oudin A, Fack F, Allega M, Sumpton D, Martinez-Garcia E, Gunnar Dittmar, Hau A, Herold-Mende C, Bjerkvig R, Meiser J, Tardito S, and Niclou S
61. DEVELOPMENT OF RUBRICS FOR ASSESSING GENERAL COMPETENCES IN SUBJECTS RELATED TO THE ENVIRONMENT AND CONSERVATION OF FOREST ECOSYSTEMS
- Author
-
Lucas-Borja, M. E., Botella Miralles, O., Lucas Borja, A., Monreal Montoya, J. A., Andres Abellan, M., Pulido Garcia, L., Garcia Morote, F. A., Martinez Garcia, E., Candel Perez, D., Lopez Serrano, F. R., Eva Rubio, and Molero Carrasco, J. more...
62. Gold mineralisation in the northwestern Iberian Peninsula.
- Author
-
Martinez-Garcia E. and Martinez-Garcia E.
- Abstract
There exists the possibility that gold could come from the igneous rocks which are considered as intrusive equivalents of the upper Devonian volcanics developed in the south Portuguese zone of southern Spain and Portugal. Gold in the NW Iberian Massif must come from deep in the lithosphere and it is possibly associated with the granulitic metamorphism preceding the intrusion of granodiorites, coming to the surface in accompanying hydrothermal solutions, in the upper Devonian as well as in the lower Permian, also in association with granodiorites intruded along deep crustal faults., There exists the possibility that gold could come from the igneous rocks which are considered as intrusive equivalents of the upper Devonian volcanics developed in the south Portuguese zone of southern Spain and Portugal. Gold in the NW Iberian Massif must come from deep in the lithosphere and it is possibly associated with the granulitic metamorphism preceding the intrusion of granodiorites, coming to the surface in accompanying hydrothermal solutions, in the upper Devonian as well as in the lower Permian, also in association with granodiorites intruded along deep crustal faults. more...
63. Development and implementation of GRASP algorithm for humanoid robot AR-601M
- Author
-
Khusnutdinov K., Sagitov A., Yakupov A., Meshcheryakov R., Hsia K., Martinez-Garcia E., Magid E., Khusnutdinov K., Sagitov A., Yakupov A., Meshcheryakov R., Hsia K., Martinez-Garcia E., and Magid E.
- Abstract
Copyright © 2019 by SCITEPRESS – Science and Technology Publications, Lda. All rights reserved In robot manipulator control, grasping different types of objects is an important task, but despite being a subject of many studies, there is still no universal approach. A humanoid robot arm end-effector has a significantly more complicated structure than the one of an industrial manipulator. It complicates a process of object grasping, but could possibly make it more robust and stable. A success of grasping strongly depends on a method of determining an object shape and a manipulator grasping procedure. Combining these factors turns object grasping by a humanoid into an interesting and versatile control problem. This paper presents a grasping algorithm for AR-601M humanoid arm with mimic joints in the hand that utilizes the simplicity of an antipodal grasp and satisfies force closure condition. The algorithm was tested in Gazebo simulation with sample objects that were modeled after selected household items. more...
64. Household objects pick and place task for AR-601M humanoid robot
- Author
-
Khusnutdinov K., Sagitov A., Yakupov A., Lavrenov R., Martinez-Garcia E., Hsia K., Magid E., Khusnutdinov K., Sagitov A., Yakupov A., Lavrenov R., Martinez-Garcia E., Hsia K., and Magid E.
- Abstract
© Springer Nature Switzerland AG 2019. Humanoid robots are created to facilitate many facets of daily life, both in scenarios when humans and robots collaborate and when robot completely replaces human. One of such more important cases is the household assistance for older people. When a robot operates in home environments the needs to interact with various household objects, of different shape and size. A humanoid end-effector is typically modeled to have from two to five configuration of fingers designed specifically for grasping. By making fingers flexible and using dexterous arm one could operate objects in many different configurations. If one chooses to provide a finger control by actuating each of the finger’s phalanxes by using separate motor, humanoid hand becomes costly and overall size of the hand will significantly increase to accommodate necessary hardware and wiring. To address this issue, many engineers prefer to employ mimic joints to reduce a cost and size, while keeping acceptable levels of finger’s dexterity. This paper presents a study on household objects pick and place task being implemented for AR-601M humanoid robot that is using mimic joints in his fingers. Experiments were conducted in a Gazebo simulation with 5 model objects, which were created to be representations of real typical household items. more...
65. Trajectory control for groups of humans by deploying a team of mobile robots
- Author
-
Martinez-Garcia, E., primary, Akihisa, O., additional, and Yuta, S., additional
- Full Text
- View/download PDF
66. Trajectory control for groups of humans by deploying a team of mobile robots
- Author
-
Martinez-Garcia, E., primary, Akihisa, O., additional, and Yuta, S., additional
- Full Text
- View/download PDF
67. pBAM1: an all-synthetic genetic tool for analysis and construction of complex bacterial phenotypes
- Author
-
Arévalo-Rodríguez Miguel, Calles Belén, Martínez-García Esteban, and de Lorenzo Víctor
- Subjects
Microbiology ,QR1-502 - Abstract
Abstract Background Since publication in 1977 of plasmid pBR322, many breakthroughs in Biology have depended on increasingly sophisticated vector platforms for analysis and engineering of given bacterial strains. Although restriction sites impose a certain format in the procedures for assembling cloned genes, every attempt thus far to standardize vector architecture and nomenclature has ended up in failure. While this state of affairs may still be tolerable for traditional one-at-a-time studies of single genes, the onset of systems and synthetic biology calls for a simplification -along with an optimization- of the currently unwieldy pool of genetic tools. Results The functional DNA sequences present in the natural bacterial transposon Tn5 have been methodically edited and refactored for the production of a multi-purpose genetic tool named pBAM1, which allows a range of manipulations in the genome of Gram-negative bacteria. This all-synthetic construct enhances the power of mini-transposon vectors for either de-construction or re-construction of phenotypes á la carte by incorporating features inspired in systems engineering: modularity, re-usability, minimization, and compatibility with other genetic tools. pBAM1 bears an streamlined, restriction site-freed and narrow-host range replication frame bearing the sequences of R6K oriV, oriT and an ampicillin resistance marker. These go along with a business module that contains a host-independent and hyperactive transposition platform for in vivo or in vitro insertion of desired DNA into the genome of the target bacterium. All functional sequences were standardized for a straightforward replacement by equivalent counterparts, if required. pBAM1 can be delivered into recipient cells by either mating or electroporation, producing transposon insertion frequencies of 1.8 × 10-3 and 1.02 × 10-7, respectively in the soil bacterium Pseudomonas putida. Analyses of the resulting clones revealed a 100% of unique transposition events and virtually no-cointegration of the donor plasmid within the target genome. Conclusions This work reports the design and performance of an all-synthetic mini-transposon vector. The power of the new system for both identification of new functions or for the construction of desired phenotypes is shown in a genetic survey of hyper-expressed proteins and regulatory elements that influence the expression of the σ54-dependent Pu promoter of P. putida. more...
- Published
- 2011
- Full Text
- View/download PDF
68. Crítica de libros
- Author
-
Martínez García, E.
- Published
- 2002
- Full Text
- View/download PDF
69. Crítica de libros
- Author
-
Martínez García, E.
- Published
- 2001
- Full Text
- View/download PDF
70. Fault development and stress evolution of the post-Hercynian Asturian Basin (Asturias and Cantabria, northwestern Spain)
- Author
-
Lepvrier, C. and Martínez-García, E.
- Published
- 1990
- Full Text
- View/download PDF
71. Palaeoenvironmental andstratigraphic aspects of animal traces and plant remains in Spanish Permian red beds (Peña Sagra, Cantabrian Mountains, Spain)
- Author
-
Gand, G., Kerp, H., Parsons, C., and Martínez-García, E.
- Published
- 1997
- Full Text
- View/download PDF
72. Cervical Fluids Are a Source of Protein Biomarkers for Early, Non-Invasive Endometrial Cancer Diagnosis
- Author
-
Martinez-Garcia, Elena, Coll-de la Rubia, Eva, Lesur, Antoine, Dittmar, Gunnar, Gil-Moreno, Antonio, Cabrera Díaz, Silvia, Colás Ortega, Eva, Universitat Autònoma de Barcelona, Institut Català de la Salut, [Martinez-Garcia E] Proteomics of Cellular Signaling, Department of Infection and Immunity, Luxembourg Institute of Health, Strassen, Luxembourg. [Coll-de la Rubia E, Colas E] Grup de Recerca Biomèdica en Ginecologia, Vall Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. CIBERONC, Barcelona, Spain. [Lesur A] Quantitative Biology Unit, Luxembourg Institute of Health, Strassen, Luxembourg. [Dittmar G] Proteomics of Cellular Signaling, Department of Infection and Immunity, Luxembourg Institute of Health, Strassen, Luxembourg. Department of Life Sciences and Medicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg. [Gil-Moreno A, Cabrera S] Grup de Recerca Biomèdica en Ginecologia, Vall Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. CIBERONC, Barcelona, Spain. Servei de Ginecologia, Vall Hebron Hospital Universitari, Barcelona, Spain. CIBERONC, Barcelona, Spain, and Vall d'Hebron Barcelona Hospital Campus more...
- Subjects
Proteomics ,Carcinoma of the endometrium ,endometrial cancer ,uterine cancer ,biomarker ,diagnosis ,gynecology ,endometrial sampling ,proteomics ,cervical sample ,protein ,non-invasive ,Cancer Research ,Protein ,Marcadors tumorals ,Otros calificadores::/diagnóstico [Otros calificadores] ,factores biológicos::biomarcadores::marcadores tumorales [COMPUESTOS QUÍMICOS Y DROGAS] ,Neoplasms::Neoplasms by Site::Urogenital Neoplasms::Genital Neoplasms, Female::Uterine Neoplasms::Endometrial Neoplasms [DISEASES] ,Biomarker ,Endometri - Càncer - Diagnòstic ,Proteòmica ,Endometrial sampling ,Endometrial cancer ,Oncology ,Gynecology ,Diagnosis ,Uterine cancer ,Other subheadings::/diagnosis [Other subheadings] ,Non-invasive ,Biological Factors::Biomarkers::Biomarkers, Tumor [CHEMICALS AND DRUGS] ,Amino Acids, Peptides, and Proteins::Proteins::Proteome [CHEMICALS AND DRUGS] ,neoplasias::neoplasias por localización::neoplasias urogenitales::neoplasias de los genitales femeninos::neoplasias uterinas::neoplasias endometriales [ENFERMEDADES] ,aminoácidos, péptidos y proteínas::proteínas::proteoma [COMPUESTOS QUÍMICOS Y DROGAS] ,Cervical sample - Abstract
Abnormal uterine bleeding (AUB) is the main symptom of endometrial cancer (EC), but it is highly nonspecific. This represents a huge burden for women's health, since all women presenting with bleeding will undergo sequential invasive tests, avoidable in 90-95% of those women who do not have EC. This study aimed to evaluate the potential of cervical samples collected with five different devices as a source of EC diagnostic protein biomarkers. Samples collected with a Rovers Cervex Brush ® and the HC2 DNA collection device, Digene, were the most suitable for EC proteomic studies. A clinical retrospective study assessing the expression of 52 EC-related proteins in 41 patients (22 EC; 19 non-EC), by targeted proteomics, identified SERPINH1, VIM, TAGLN, PPIA, CSE1L, and CTNNB1 as potential EC protein biomarkers in cervical fluids (AUC > 0.8). This study opens an avenue for developing non-invasive protein-based EC diagnostic tests, which will improve the standard of care for gynecological patients. Background: Abnormal uterine bleeding is the main symptom of endometrial cancer (EC), but it is highly nonspecific. This represents a huge burden for women's health since all women presenting with bleeding will undergo sequential invasive tests, which are avoidable for 90-95% of those women who do not have EC. Methods: This study aimed to evaluate the potential of cervical samples collected with five different devices as a source of protein biomarkers to diagnose EC. We evaluated the protein quantity and the proteome composition of five cervical sampling methods. Results: Samples collected with a Rovers Cervex Brush ® and the HC2 DNA collection device, Digene, were the most suitable samples for EC proteomic studies. Most proteins found in uterine fluids were also detected in both cervical samples. We then conducted a clinical retrospective study to assess the expression of 52 EC-related proteins in 41 patients (22 EC; 19 non-EC), using targeted proteomics. We identified SERPINH1, VIM, TAGLN, PPIA, CSE1L, and CTNNB1 as potential protein biomarkers to discriminate between EC and symptomatic non-EC women with abnormal uterine bleeding in cervical fluids (AUC > 0.8). Conclusions: This study opens an avenue for developing non-invasive protein-based EC diagnostic tests, which will improve the standard of care for gynecological patients. more...
- Published
- 2023
- Full Text
- View/download PDF
73. In silico Approach for Validating and Unveiling New Applications for Prognostic Biomarkers of Endometrial Cancer
- Author
-
Petr V. Nazarov, Silvia Cabrera, Eva Coll-de la Rubia, Antonio Gil-Moreno, Eva Colas, Gunnar Dittmar, Elena Martinez-Garcia, Vicente Bebia, Institut Català de la Salut, [Coll-de la Rubia E, Colás E] Grup de Recerca Biomèdica en Ginecologia, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. CIBERONC Barcelona, Spain. [Martinez-Garcia E, Dittmar G, Nazarov PV] Luxembourg Institute of Health, L-1445 Strassen, Luxembourg. [Bebia V] Servei de Ginecologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. CIBERONC, Barcelona, Spain. [Cabrera S, Gil-Moreno A] Grup de Recerca Biomèdica en Ginecologia, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. CIBERONC, Barcelona, Spain. Servei de Ginecologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. CIBERONC, Barcelona, Spain, and Vall d'Hebron Barcelona Hospital Campus more...
- Subjects
Oncology ,Cancer Research ,medicine.medical_specialty ,CPTAC ,In silico ,Otros calificadores::/diagnóstico [Otros calificadores] ,Article ,uterine cancer ,Molecular classification ,Uterine cancer ,Information Science::Computing Methodologies::Computer Simulation [INFORMATION SCIENCE] ,high-risk ,Internal medicine ,Ciencias de la información::metodologías computacionales::simulación por ordenador [CIENCIA DE LA INFORMACIÓN] ,medicine ,Other subheadings::/diagnosis [Other subheadings] ,Stage (cooking) ,Diagnosis::Prognosis [ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT] ,prognostic biomarker ,diagnóstico::pronóstico [TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS] ,RC254-282 ,Endometri - Càncer - Prognosi ,business.industry ,Endometrial cancer ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Neoplasms::Neoplasms by Site::Urogenital Neoplasms::Genital Neoplasms, Female::Uterine Neoplasms::Endometrial Neoplasms [DISEASES] ,bioinformatics ,TCGA ,medicine.disease ,MSH6 ,Expression data ,MSH2 ,endometrial cancer ,Simulació (Medicina) ,business ,neoplasias::neoplasias por localización::neoplasias urogenitales::neoplasias de los genitales femeninos::neoplasias uterinas::neoplasias endometriales [ENFERMEDADES] - Abstract
Simple Summary Endometrial cancer (EC) mortality is directly associated with the presence of poor prognostic factors. Molecular prognostic factors have been identified, but none are used in clinical practice due to lack of validation studies. This study aims to validate a set of 255 prognostic biomarkers previously identified in an extensive literature review and explore new prognostic applications by analyzing them in The Cancer Genome Atlas (TCGA) and Clinical Proteomic Tumor Analysis Consortium (CPTAC) databases. A total of 30 biomarkers were validated and associated to a histological type (n = 15), histological grade (n = 6), FIGO stage (n = 1), molecular classification (n = 16), overall survival (n = 11), and recurrence-free survival (n = 5). Our results encourage further studies of understudied biomarkers such as TPX2, and validates already broadly studied biomarkers such as MSH6, MSH2, or L1CAM, among others. Finally, our results present a significant step to advance the quest for biomarkers to accurately assess the risk of EC patients. Abstract Endometrial cancer (EC) mortality is directly associated with the presence of prognostic factors. Current stratification systems are not accurate enough to predict the outcome of patients. Therefore, identifying more accurate prognostic EC biomarkers is crucial. We aimed to validate 255 prognostic biomarkers identified in multiple studies and explore their prognostic application by analyzing them in TCGA and CPTAC datasets. We analyzed the mRNA and proteomic expression data to assess the statistical prognostic performance of the 255 proteins. Significant biomarkers related to overall survival (OS) and recurrence-free survival (RFS) were combined and signatures generated. A total of 30 biomarkers were associated either to one or more of the following prognostic factors: histological type (n = 15), histological grade (n = 6), FIGO stage (n = 1), molecular classification (n = 16), or they were associated to OS (n = 11), and RFS (n = 5). A prognostic signature composed of 11 proteins increased the accuracy to predict OS (AUC = 0.827). The study validates and identifies new potential applications of 30 proteins as prognostic biomarkers and suggests to further study under-studied biomarkers such as TPX2, and confirms already used biomarkers such as MSH6, MSH2, or L1CAM. These results are expected to advance the quest for biomarkers to accurately assess the risk of EC patients. more...
- Published
- 2021
74. Corrigendum to ‘Difficult tracheal intubation in neonates and infants. NEonate and Children audiT of Anaesthesia pRactice IN Europe (NECTARINE):a prospective European multicentre observational study’ (Br J Anaesth 2021; 126: 1173–81) (British Journal of Anaesthesia (2021) 126(6) (1173–1181), (S0007091221001161), (10.1016/j.bja.2021.02.021))
- Author
-
Nicola Disma, Katalin Virag, Thomas Riva, Jost Kaufmann, Thomas Engelhardt, Walid Habre, Christian Breschan, Rudolf Likar, Manuela Platzer, Isole Edelman, Johanes Eger, Stefan Heschl, Brigitte Messerer, Maria Vittinghof, Ruth Kroess, Martina Stichlberger, David Kahn, Thierry Pirotte, Caroline Pregardien, Francis Veyckemans, France Stevens, Johan Berghmans, Annemie Bauters, Luc De Baerdemaeker, Stefan De Hert, Koen Lapage, Aliaksandra Parashchanka, Jurgen Van Limmen, Piet Wyffels, Julie Lauweryns, Nadia Najafi, Joris Vundelinckx, Diana Butković, Ivana Kerovec Sorić, Sandra Kralik, Ana Markić, Josip Azman, Josko Markic, Daniela Pupacic, Michal Frelich, Petr Reimer, René Urbanec, Petra Cajková, Vladimír Mixa, Yvona Sedláčková, Lenka Knoppová, Alena Zlámalová (neé Květoňová), Martin Vavřina, Jiří Žurek, Tom Hansen, Arash Afshari, Anders Bastholm Bille, Marguerite Ellekvist, Mari-Liis Ilmoja, Reet Moor, Reet Kikas, Merle Väli, Kariantti Kallio, Elisa Reponen, Pertti Suominen, Sami Suvanto, Raisa Vähätalo, Hannu Kokki, Merja Kokki, Jarkko Harju, Miia Kokkonen, Jenni Vieri, Tuula Manner, Catherine Amory, Hugues Ludot, Dina Bert, Juliette Godart, Anne Laffargue, Hervé Dupont, Benjamin Urbina, Catherine Baujard, Philippe Roulleau, Giuseppe Staiti, Maryline Bordes, Karine Nouette Gaulain, Yann Hamonic, François Semjen, Olivier Jacqmarcq, Caroline Lejus-Bourdeau, Cécile Magne, Léa Petry, Lilica Ros, Aurélien Zang, Mehdi Bennis, Bernard Coustets, Rose Fesseau, Isabelle Constant, Eliane Khalil, Nada Sabourdin, Noemie Audren, Thomas Descarpentries, Fanny Fabre, Aurélien Legrand, Emilie Druot, Gilles Orliaguet, Lucie Sabau, Lynn Uhrig, François de la Brière, Karin Jonckheer, Jean-Paul Mission, Lucia Scordo, Caroline Couchepin, Christophe Dadure, Pablo De la Arena, Laurent Hertz, Philippe Pirat, Chrystelle Sola, Myriam Bellon, Souhayl Dahmani, Florence Julien-Marsollier, Daphne Michelet, Veronique Depret-Donatien, Anne Lesage, Michael Laschat, Frank Wappler, Karin Becke, Lena Brunner, Karin Oppenrieder, Gregor Badelt, Karin Hochmuth, Bernhard Koller, Anita Reil, Sebastian Richter, Thomas Fischer, Anja Diers, Clemens Schorer, Andreas Weyland, Ruth Cohausz, Franz-Josef Kretz, Michaela Löffler, Markus Wilbs, Claudia Hoehne, Johanna Ulrici, Christiane Goeters, Armin Flinspach, Matthias Klages, Simone Lindau, Leila Messroghli, Kai Zacharowski, Christoph Eisner, Thomas Mueller, Daniel Richter, Melanie Schäfer, Markus Weigand, Sebastian Weiterer, Miriam Ochsenreiter, Michael Schöler, Tom Terboven, Isabel Eggemann, Sascha Haussmann, Nicolas Leister, Christoph Menzel, Uwe Trieschmann, Sirin Yücetepe, Susanna Keilig, Peter Kranke, Yvonne Jelting, Torsten Baehner, Richard Ellerkmann, Shahab Ghamari, Claudia Neumann, Martin Söhle, Pelagia Chloropoulou, Vagia Ntritsou, Pinelopi Papagiannopoulou, Eleana Garini, Afroditi Karafotia, Panagoula Mammi, Evangelia Bali, Despoina Iordanidou, Anna Malisiova, Artemis Polyzoi, Adelais Tsiotou, Erzsebet Sapi, Edgar Székely, Nandor Kosik, Veronika Maráczi, Janos Schnur, Judit Csillag, János Gál, Gergely Göbl, Balázs Hauser, András Petróczy, Gyula Tövisházi, Stuart Blain, Sarah Gallagher, Sinead Harte, Mandy Jackson, Emma Meehan, Zeenat Nawoor, Brendan O’Hare, Mark Ross, Daniela Lerro, Marinella Astuto, Chiara Grasso, Rita Scalisi, Giulia Frasacco, Elena Lenares, Roberto Leone, Maurizia Grazzini, Carmelo Minardi, Nicola Zadra, Gilda Cinnella, Antonella Cotoia, Dario Galante, Brita De Lorenzo, Beate Kuppers, Giulia Bottazzi, Fabio Caramelli, Maria Cristina Mondardini, Emanuele Rossetti, Sergio Picardo, Alessandro Vittori, Anna Camporesi, Andrea Wolfler, Edoardo Calderini, Laura Brigitta Colantonio, Simona Anna Finamore, Giuliana Anna Porro, Rachele Bonfiglio, Svetlana Kotzeva, Leila Mameli, Girolamo Mattioli, Camilla Micalizzi, Alessia Montaguti, Angela Pistorio, Clelia Zanaboni, Anna Guddo, Gerald Rogan Neba, Moreno Favarato, Bruno Guido Locatelli, Micol Maffioletti, Valter Sonzogni, Rossella Garra, Maria Sammartino, Fabio Sbaraglia, Andrea Cortegiani, Alessandra Moscarelli, Elena Attanasi, Simonetta Tesoro, Cristina Agapiti, Francesca Pinzoni, Cesare Vezzoli, Federico Bilotta, Arta Barzdina, Zane Straume, Anda Zundane, Laura Lukosiene, Irena Maraulaite, Ilona Razlevice, Bernd Schmitz, Stephanie Mifsud, Carolin Aehling, Celia Allison, Rients De Boer, Dina Emal, Markus Stevens, Marielle Buitenhuis, Jurgen de Graaff, Inge De Liefde, Andreas Machotta, Gail Scoones, Lonneke Staals, Jeremy Tomas, Anouk Van der Knijff-van Dortmont, Marianne Veldhuizen, David Alders, Wolfgang Buhre, Eva Schafrat, Jan Schreiber, Petronella Mari Vermeulen, Mark Hendriks, Sandra Lako, Marieke Voet-Lindner, Barbe Pieters, Gert-Jan Scheffer, Luc Tielens, Anthony R. Absalom, Margot Bergsma, Joke De Ruiter, Sascha Meier, Martin Volkers, Tjerk Zweers, Anne M. Beukers, Christa Boer, Jurgen Dertinger, Sandra Numan, Bas Van Zaane, Wenche B. Boerke, Nil Ekiz, Kristoffer Stensrud, Inger Marie Drage, Erik Ramon Isern, Alicja Bartkowska-Sniatkowska, Malgorzata Grzeskowiak, Magdalena Juzwa-Sobieraj, Jowita Rosada-Kurasińska, Artur Baranowski, Karina Jakubowska, Dorota Lewandowska, Magdalena Mierzewska-Schmidt, Piotr Sawicki, Magdalena Urban-Lechowicz, Pomianek Przemyslaw, Marzena Zielinska, Teresa Leal, Maria Soares, Pedro Pina, Sílvia Pinho, Maria Domingas Patuleia, Catarina Cruz Esteves, Helena Salgado, Maria João Santos, Rodica Badeti, Iulia Cindea, Loredana Oana, Adriana Gurita, Luminita Ilie, Gabriel Mocioiu, Radu Tabacaru, Irina Trante, Valentin Munteanu, Mihai Morariu, Emese Nyíri, Ivana Budic, Vesna Marjanovic, Biljana Drašković, Marina Pandurov, Jordanka Ilic, Ana Mandras, Zdenka Rados, Nikola Stankovic, Maja Suica, Sladjana Vasiljevic, Mirjana Knezevic, Irina Milojevic, Ivana Petrov, Selena Puric Racic, Dusica Simic, Irena Simic, Marija Stevic, Irena Vulicevic, Barbora Cabanová, Miloslav Hanula, Jelena Berger, Darja Janjatovic, Špela Pirtovšek Štupnik, Dolores Méndez, Gema Pino, Paloma Rubio, Alberto Izquierdo, Silvia López, Cristina González Serrano, Jesús Cebrián, Ana Peleteiro, Pilar Del Rey de Diego, Ernesto Martínez García, Carolina Tormo de las Heras, Pablo Troncoso Montero, Celia Arbona, David Artés, Alicia Chamizo, Silvia Serrano, Montserrat Suarez Comas, Francisco Escribá, Cristina Auli, Osvaldo Pérez Pardo, Natalia Sierra Biddle, Ceferina Suárez Castaño, María Isabel Villalobos Rico, Susana Manrique Muñoz, Irene García Martínez, Nuria Montferrer Estruch, Elena Vilardell Ortíz, Rodrigo Poves-Álvarez, Ivan Kohn, Ulf Lindestam, Jarl Reinhard, Albert Castellheim, Kerstin Sandström, Sporre Bengt, Rainer Dörenberg, Peter Frykholm, Maria Garcia, Ann Kvarnström, Emma Pontén, Thomas Bruelisauer, Gabor Erdoes, Heiko Kaiser, Mathias Marchon, Stefan Seiler, Yann Bögli, Mirko Dolci, Carine Marcucci, Isabelle Pichon, Laszlo Vutskits, Mattias Casutt, Martin Hölzle, Thomas Hurni, Martin Jöhr, Anna-Ursina Malär, Jacqueline Mauch, Thomas Erb, Karin Oeinck, Mine Akin, Gulsen Keskin, Yesim Senayli, Guner Kaya, Pinar Kendigelen, Ayse Çiğdem Tutuncu, Zehra Hatipoğlu, Dilek Özcengiz, Hale Aksu Erdost, Elvan Öçmen, Çimen Olguner, Hilmi Ayanoglu, Pelin Corman Dincer, Tumay Umuroglu, Mustafa Azizoglu, Handan Birbiçer, Nurcan Doruk, Aslı Sagun, Sibel Baris, Dmytro Dmytriiev, Sridevi Kuchi, Nuria Masip, Peter Brooks, Alison Hare, Nargis Ahmad, Michelle Casey, Sam De Silva, Nadine Dobby, Prakash Krishnan, L. Amaki Sogbodjor, Ellie Walker, Suellen Walker, Stephanie King, Katy Nicholson, Michelle Quinney, Paul Stevens, Andrew Blevin, Mariangela Giombini, Chulananda Goonasekera, Sadia Adil, Stephanie Bew, Carol Bodlani, Dan Gilpin, Stephanie Jinks, Nalini Malarkkan, Alice Miskovic, Rebecca Pad, Juliet Wolfe Barry, Joy Abbott, James Armstrong, Natalie Cooper, Lindsay Crate, John Emery, Kathryn James, Hannah King, Paul Martin, Stefano Scalia Catenacci, Rob Bomont, Paul Smith, Sara Mele, Alessandra Verzelloni, Philippa Dix, Graham Bell, Elena Gordeva, Lesley McKee, Esther Ngan, Jutta Scheffczik, Li-En Tan, Mark Worrall, Carmel Cassar, Kevin Goddard, Victoria Barlow, Vimmi Oshan, Khairi Shah, Sarah Bell, Lisa Daniels, Monica Gandhi, David Pachter, Chris Perry, Andrew Robertson, Carmen Scott, Lynne Waring, David Barnes, Sophie Childs, Joanne Norman, Robin Sunderland, Dowell Julia, Feijten Prisca, Harlet Pierre, Herbineaux Sarah, Leva Brigitte, Plichon Benoît, Virág Katalin, Disma N., Virag K., Riva T., Kaufmann J., Engelhardt T., Habre W., Breschan C., Likar R., Platzer M., Edelman I., Eger J., Heschl S., Messerer B., Vittinghof M., Kroess R., Stichlberger M., Kahn D., Pirotte T., Pregardien C., Veyckemans F., Stevens F., Berghmans J., Bauters A., De Baerdemaeker L., De Hert S., Lapage K., Parashchanka A., Van Limmen J., Wyffels P., Lauweryns J., Najafi N., Vundelinckx J., Butkovic D., Kerovec Soric I., Kralik S., Markic A., Azman J., Markic J., Pupacic D., Frelich M., Reimer P., Urbanec R., Cajkova P., Mixa V., Sedlackova Y., Knoppova L., Zlamalova (nee Kvetonova) A., Vavrina M., Zurek J., Hansen T., Afshari A., Bille A.B., Ellekvist M., Ilmoja M.-L., Moor R., Kikas R., Vali M., Kallio K., Reponen E., Suominen P., Suvanto S., Vahatalo R., Kokki H., Kokki M., Harju J., Kokkonen M., Vieri J., Manner T., Amory C., Ludot H., Bert D., Godart J., Laffargue A., Dupont H., Urbina B., Baujard C., Roulleau P., Staiti G., Bordes M., Nouette Gaulain K., Hamonic Y., Semjen F., Jacqmarcq O., Lejus-Bourdeau C., Magne C., Petry L., Ros L., Zang A., Bennis M., Coustets B., Fesseau R., Constant I., Khalil E., Sabourdin N., Audren N., Descarpentries T., Fabre F., Legrand A., Druot E., Orliaguet G., Sabau L., Uhrig L., de la Briere F., Jonckheer K., Mission J.-P., Scordo L., Couchepin C., Dadure C., De la Arena P., Hertz L., Pirat P., Sola C., Bellon M., Dahmani S., Julien-Marsollier F., Michelet D., Depret-Donatien V., Lesage A., Laschat M., Wappler F., Becke K., Brunner L., Oppenrieder K., Badelt G., Hochmuth K., Koller B., Reil A., Richter S., Fischer T., Diers A., Schorer C., Weyland A., Cohausz R., Kretz F.-J., Loffler M., Wilbs M., Hoehne C., Ulrici J., Goeters C., Flinspach A., Klages M., Lindau S., Messroghli L., Zacharowski K., Eisner C., Mueller T., Richter D., Schafer M., Weigand M., Weiterer S., Ochsenreiter M., Scholer M., Terboven T., Eggemann I., Haussmann S., Leister N., Menzel C., Trieschmann U., Yucetepe S., Keilig S., Kranke P., Jelting Y., Baehner T., Ellerkmann R., Ghamari S., Neumann C., Sohle M., Chloropoulou P., Ntritsou V., Papagiannopoulou P., Garini E., Karafotia A., Mammi P., Bali E., Iordanidou D., Malisiova A., Polyzoi A., Tsiotou A., Sapi E., Szekely E., Kosik N., Maraczi V., Schnur J., Csillag J., Gal J., Gobl G., Hauser B., Petroczy A., Tovishazi G., Blain S., Gallagher S., Harte S., Jackson M., Meehan E., Nawoor Z., O'Hare B., Ross M., Lerro D., Astuto M., Grasso C., Scalisi R., Frasacco G., Lenares E., Leone R., Grazzini M., Minardi C., Zadra N., Cinnella G., Cotoia A., Galante D., De Lorenzo B., Kuppers B., Bottazzi G., Caramelli F., Mondardini M.C., Rossetti E., Picardo S., Vittori A., Camporesi A., Wolfler A., Calderini E., Colantonio L.B., Finamore S.A., Porro G.A., Bonfiglio R., Kotzeva S., Mameli L., Mattioli G., Micalizzi C., Montaguti A., Pistorio A., Zanaboni C., Guddo A., Neba G.R., Favarato M., Locatelli B.G., Maffioletti M., Sonzogni V., Garra R., Sammartino M., Sbaraglia F., Cortegiani A., Moscarelli A., Attanasi E., Tesoro S., Agapiti C., Pinzoni F., Vezzoli C., Bilotta F., Barzdina A., Straume Z., Zundane A., Lukosiene L., Maraulaite I., Razlevice I., Schmitz B., Mifsud S., Aehling C., Allison C., De Boer R., Emal D., Stevens M., Buitenhuis M., de Graaff J., De Liefde I., Machotta A., Scoones G., Staals L., Tomas J., Van der Knijff-van Dortmont A., Veldhuizen M., Alders D., Buhre W., Schafrat E., Schreiber J., Vermeulen P.M., Hendriks M., Lako S., Voet-Lindner M., Pieters B., Scheffer G.-J., Tielens L., Absalom A.R., Bergsma M., De Ruiter J., Meier S., Volkers M., Zweers T., Beukers A.M., Boer C., Dertinger J., Numan S., Van Zaane B., Boerke W.B., Ekiz N., Stensrud K., Drage I.M., Isern E.R., Bartkowska-Sniatkowska A., Grzeskowiak M., Juzwa-Sobieraj M., Rosada-Kurasinska J., Baranowski A., Jakubowska K., Lewandowska D., Mierzewska-Schmidt M., Sawicki P., Urban-Lechowicz M., Przemyslaw P., Zielinska M., Leal T., Soares M., Pina P., Pinho S., Patuleia M.D., Esteves C.C., Salgado H., Santos M.J., Badeti R., Cindea I., Oana L., Gurita A., Ilie L., Mocioiu G., Tabacaru R., Trante I., Munteanu V., Morariu M., Nyiri E., Budic I., Marjanovic V., Draskovic B., Pandurov M., Ilic J., Mandras A., Rados Z., Stankovic N., Suica M., Vasiljevic S., Knezevic M., Milojevic I., Petrov I., Puric Racic S., Simic D., Simic I., Stevic M., Vulicevic I., Cabanova B., Hanula M., Berger J., Janjatovic D., Pirtovsek Stupnik S., Mendez D., Pino G., Rubio P., Izquierdo A., Lopez S., Gonzalez Serrano C., Cebrian J., Peleteiro A., Del Rey de Diego P., Martinez Garcia E., Tormo de las Heras C., Troncoso Montero P., Arbona C., Artes D., Chamizo A., Serrano S., Suarez Comas M., Escriba F., Auli C., Perez Pardo O., Sierra Biddle N., Suarez Castano C., Villalobos Rico M.I., Manrique Munoz S., Garcia Martinez I., Montferrer Estruch N., Vilardell Ortiz E., Poves-Alvarez R., Kohn I., Lindestam U., Reinhard J., Castellheim A., Sandstrom K., Bengt S., Dorenberg R., Frykholm P., Garcia M., Kvarnstrom A., Ponten E., Bruelisauer T., Erdoes G., Kaiser H., Marchon M., Seiler S., Bogli Y., Dolci M., Marcucci C., Pichon I., Vutskits L., Casutt M., Holzle M., Hurni T., Johr M., Malar A.-U., Mauch J., Erb T., Oeinck K., Akin M., Keskin G., Senayli Y., Kaya G., Kendigelen P., Tutuncu A.C., Hatipoglu Z., Ozcengiz D., Erdost H.A., Ocmen E., Olguner C., Ayanoglu H., Dincer P.C., Umuroglu T., Azizoglu M., Birbicer H., Doruk N., Sagun A., Baris S., Dmytriiev D., Kuchi S., Masip N., Brooks P., Hare A., Ahmad N., Casey M., De Silva S., Dobby N., Krishnan P., Sogbodjor L.A., Walker E., Walker S., King S., Nicholson K., Quinney M., Stevens P., Blevin A., Giombini M., Goonasekera C., Adil S., Bew S., Bodlani C., Gilpin D., Jinks S., Malarkkan N., Miskovic A., Pad R., Wolfe Barry J., Abbott J., Armstrong J., Cooper N., Crate L., Emery J., James K., King H., Martin P., Scalia Catenacci S., Bomont R., Smith P., Mele S., Verzelloni A., Dix P., Bell G., Gordeva E., McKee L., Ngan E., Scheffczik J., Tan L.-E., Worrall M., Cassar C., Goddard K., Barlow V., Oshan V., Shah K., Bell S., Daniels L., Gandhi M., Pachter D., Perry C., Robertson A., Scott C., Waring L., Barnes D., Childs S., Norman J., Sunderland R., Julia D., Prisca F., Pierre H., Sarah H., Brigitte L., Benoit P., Katalin V., Anesthesiology, APH - Quality of Care, and Amsterdam Neuroscience - Neuroinfection & -inflammation more...
- Subjects
Anesthesiology and Pain Medicine ,business.industry ,medicine.medical_treatment ,Anesthesia ,Tracheal intubation ,Neonates, anaesthesia ,medicine ,MEDLINE ,Observational study ,Audit ,business - Abstract
The authors regret that errors were present in the above article. On page 1174, in the second paragraph of the Statistical methods section, the second sentence should read as follows: The incidence of difficult intubation was determined including those whose tracheas were already intubated and is reported as a percentage with a 95% exact binomial CI. On page 1175, in the third paragraph of the Statistical methods section ‘mean standardised difference (MSD)’ should read ‘standardised mean difference (SMD)’ The authors would like to apologise for any inconvenience caused. more...
- Published
- 2021
- Full Text
- View/download PDF
75. Factors associated with adverse COVID-19 outcomes in patients with psoriasis—insights from a global registry–based study
- Author
-
Silvia Pérez-Barrio, Lucy Moorhead, Manpreet Lakhan, Saskia Reeken, Vito Zeeshaan Hasab, Rogelio Mercado-Seda, Gustavo Anibal Cardozo, Georgi Popov, Enrique Loayza, Marie-Louise Svensson, Emmanuel Mahe, Fernando Valenzuela, Victoria King, Michela Magnano, Danielle Brassard, Annette Essex, Deanna Cummings, Manisha Panchal, Trupti V. Desai, Jennifer E. Carolan, Areti Makrygeorgou, Zenas Z N Yiu, Teena Mackenzie, Esteban Daudén, Emmanuel Toni, Ian Pearson, Andrea Carugno, Lorraine Gribben, Leontien de Graaf, Liv Eidsmo, Esther A. Balogh, Gloria Aparicio, Andrew Pink, Manel Velasco, Adrienne J. van Geest, Steven R. Feldman, Tiago Torres, Elzbieta Klujszo, Malcolm H.A. Rustin, Ignacio Yanguas, Anthony Bewley, Eliseo Martínez-García, Benhadou Farida, Emily Dwyer, Susannah Hoey, Richard B. Warren, Esther E. Freeman, Diana Ruiz Genao, Rohima Khatun, Giulia Rech, Elena B. Hawryluk, Zahira Koreja, Ricardo Romiti, Gonzalez A. Cesar, Alice Mwale, Charlotte Barclay, Aadarsh Shah, Catherine Quinlan, Kathryn G. Kerisit, Christopher E.M. Griffiths, Carla Tubau Prims, Lone Skov, Céline Phan, Vincent Descamps, Jenny Hughes, Siew Eng Choon, Shanti Ayob, Efrossini Carras, Girard Celine, Jo Lambert, Alberto Barea, Jonathan Barker, Reinhart Speeckaert, Raquel Rivera, Portia Goldsmith, Nick Dand, Beatriz Pérez-Suárez, Andrew DeCrescenzo, F. Meynell, Francesca Capon, Toomas Talme, Teresa Tsakok, Deepti Kolli, Stefano Piaserico, Jamie Weisman, Manuel D. Franco, K.J. Mason, Pablo De Caso, Catriona Maybury, Rachel Bak, Ann Sergeant, Keith Wu, Graham A. Johnston, Alexandra Paolino, Cécile Lesort, Mark Vandaele, H. McAteer, Birgitta Wilson Claréus, Sinead Langan, Jose-Manuel Carrascosa, Enikö Sonkoly, Claudia de la Cruz, Maruska Marovt, Luigi Naldi, Leila Asfour, Paola Di Meglio, Jose-Maria Ortiz-Salvador, Alekya Singapore, Peter Jenkin, Romana Ceovic, R. Taberner, P.J. Hampton, Alberto Romero-Maté, Russell W. Cohen, Omid Zargari, Maria Teresa Rossi, Devon E. McMahon, Denis Jullien, Bola Coker, Carrie Davis, Georgie King, Catherine H. Smith, Richard Woolf, Luis Puig, Ann Jones, Astrid van Huizen, Joseph J. Schwartz, Paolo Gisondi, Phyllis I. Spuls, Satveer K. Mahil, Sarah Kirk, Paulo Varela, K. Jackson, Ana Maria Morales Callaghan, Vito Di Lernia, Lieve Meuleman, Claudio Greco, Simina Stefanescu, Hervé Bachelez, Ana Martinez, Dermatology, AII - Inflammatory diseases, APH - Methodology, APH - Quality of Care, Mahil, S, Dand, N, Mason, K, Yiu, Z, Tsakok, T, Meynell, F, Coker, B, Mcateer, H, Moorhead, L, Mackenzie, T, Rossi, M, Rivera, R, Mahe, E, Carugno, A, Magnano, M, Rech, G, Balogh, E, Feldman, S, De La Cruz, C, Choon, S, Naldi, L, Lambert, J, Spuls, P, Jullien, D, Bachelez, H, Mcmahon, D, Freeman, E, Gisondi, P, Puig, L, Warren, R, Di Meglio, P, Langan, S, Capon, F, Griffiths, C, Barker, J, Smith, C, Shah, A, Barea, A, Romero-Mate, A, Singapore, A, Paolino, A, Mwale, A, Morales Callaghan, A, Martinez, A, Decrescenzo, A, Pink, A, Jones, A, Sergeant, A, Essex, A, Bewley, A, Makrygeorgou, A, van Huizen, A, Perez-Suarez, B, Farida, B, Clareus, B, Prims, C, Davis, C, Quinlan, C, Maybury, C, Cesar, G, Barclay, C, Greco, C, Brassard, D, Cummings, D, Kolli, D, Descamps, V, Genao, D, Carras, E, Hawryluk, E, Martinez-Garcia, E, Klujszo, E, Dwyer, E, Toni, E, Sonkoly, E, Loayza, E, Dauden, E, Valenzuela, F, Popov, G, King, G, Celine, G, Aparicio, G, Johnston, G, Cardozo, G, Pearson, I, Yanguas, I, Weisman, J, Carolan, J, Hughes, J, Ortiz-Salvador, J, Carrascosa, J, Schwartz, J, Jackson, K, Kerisit, K, Wu, K, Asfour, L, de Graaf, L, Lesort, C, Meuleman, L, Eidsmo, L, Skov, L, Gribben, L, Rustin, M, Velasco, M, Panchal, M, Lakhan, M, Franco, M, Svensson, M, Vandaele, M, Marovt, M, Zargari, O, De Caso, P, Varela, P, Jenkin, P, Phan, C, Hampton, P, Goldsmith, P, Bak, R, Speeckaert, R, Romiti, R, Woolf, R, Mercado-Seda, R, Khatun, R, Ceovic, R, Taberner, R, Cohen, R, Stefanescu, S, Kirk, S, Reeken, S, Ayob, S, Perez-Barrio, S, Piaserico, S, Hoey, S, Torres, T, Talme, T, Desai, T, van Geest, A, King, V, Di Lernia, V, Koreja, Z, and Hasab, V more...
- Subjects
Male ,IMID, immune-mediated inflammatory disease ,immunosuppressant ,BMI, body mass index ,ACEi, angiotensin-converting enzyme inhibitor ,PsoProtect, Psoriasis Patient Registry for Outcomes, Therapy and Epidemiology of COVID-19 infecTion ,Logistic regression ,Systemic therapy ,030207 dermatology & venereal diseases ,0302 clinical medicine ,RC705 ,Interquartile range ,COVID-19 ,biologics ,hospitalization ,immunosuppressants ,psoriasis ,risk factors ,Risk Factors ,Epidemiology ,Immunology and Allergy ,030212 general & internal medicine ,Registries ,NSAID, non-steroidal anti-inflammatory drug ,610 Medicine & health ,COVID-19, Coronavirus disease 2019 ,TNF, tumor necrosis factor ,Age Factors ,Middle Aged ,Hospitalization ,risk factor ,95% CI, 95% confidence interval ,Female ,JAK, Janus kinase ,biologic ,Adult ,medicine.medical_specialty ,Immunology ,Lower risk ,SARS-CoV-2, severe acute respiratory syndrome coronavirus 2 ,Article ,03 medical and health sciences ,Sex Factors ,Internal medicine ,Psoriasis ,medicine ,Humans ,SARS-CoV-2 ,IFN, interferon ,IQR, interquartile range ,psoriasi ,business.industry ,Odds ratio ,medicine.disease ,ARB, angiotensin II receptor blocker ,IL, interleukin ,OR, odds ratio ,business ,Body mass index - Abstract
Background The multi-morbid burden and use of systemic immunosuppressants in people with psoriasis may confer greater risk of adverse COVID-19 outcomes but data are limited. Objective Characterize the course of COVID-19 in psoriasis and identify factors associated with hospitalization. Methods Clinicians reported psoriasis patients with confirmed/suspected COVID-19 via an international registry, PsoProtect. Multiple logistic regression assessed the association between clinical/demographic characteristics and hospitalization. A separate patient-facing registry characterized risk-mitigating behaviours. Results Of 374 clinician-reported patients from 25 countries, 71% were receiving a biologic, 18% a non-biologic and 10% no systemic treatment for psoriasis. 348 (93%) fully recovered from COVID-19, 77 (21%) were hospitalized and nine (2%) died. Increased hospitalization risk was associated with older age (multivariable-adjusted OR 1.59 per 10 years, 95% CI 1.19-2.13), male sex (OR 2.51, 95% CI 1.23-5.12), non-white ethnicity (OR 3.15, 95% CI 1.24-8.03) and comorbid chronic lung disease (OR 3.87, 95% CI 1.52-9.83). Hospitalization was more frequent in patients using non-biologic systemic therapy than biologics (OR 2.84, 95% CI 1.31-6.18). No significant differences were found between biologic classes. Independent patient-reported data (n=1,626 across 48 countries) suggested lower levels of social isolation in individuals receiving non-biologic systemic therapy compared to biologics (OR 0.68, 95% CI 0.50-0.94). Conclusion In this international moderate-severe psoriasis case series, biologics use was associated with lower risk of COVID-19-related hospitalization than non-biologic systemic therapies, however further investigation is warranted due to potential selection bias and unmeasured confounding. Established risk factors (being older, male, non-white ethnicity, comorbidities) were associated with higher hospitalization rates. Clinical Implications We identify risk factors for COVID-19-related hospitalization in psoriasis patients, including older age, male sex, non-white ethnicity and comorbidities. Use of biologics was associated with lower hospitalization risk than non-biologic systemic therapies., Capsule summary: In this global registry-based study, risk factors for COVID-19-related hospitalization in psoriasis patients were older age, male sex, non-white ethnicity and comorbidities. Use of biologics was associated with lower hospitalization risk than non-biologic systemic treatment. more...
- Published
- 2021
- Full Text
- View/download PDF
76. In silico Approach for Validating and Unveiling New Applications for Prognostic Biomarkers of Endometrial Cancer.
- Author
-
Coll-de la Rubia E, Martinez-Garcia E, Dittmar G, Nazarov PV, Bebia V, Cabrera S, Gil-Moreno A, and Colás E
- Abstract
Endometrial cancer (EC) mortality is directly associated with the presence of prognostic factors. Current stratification systems are not accurate enough to predict the outcome of patients. Therefore, identifying more accurate prognostic EC biomarkers is crucial. We aimed to validate 255 prognostic biomarkers identified in multiple studies and explore their prognostic application by analyzing them in TCGA and CPTAC datasets. We analyzed the mRNA and proteomic expression data to assess the statistical prognostic performance of the 255 proteins. Significant biomarkers related to overall survival (OS) and recurrence-free survival (RFS) were combined and signatures generated. A total of 30 biomarkers were associated either to one or more of the following prognostic factors: histological type ( n = 15), histological grade ( n = 6), FIGO stage ( n = 1), molecular classification ( n = 16), or they were associated to OS ( n = 11), and RFS ( n = 5). A prognostic signature composed of 11 proteins increased the accuracy to predict OS (AUC = 0.827). The study validates and identifies new potential applications of 30 proteins as prognostic biomarkers and suggests to further study under-studied biomarkers such as TPX2, and confirms already used biomarkers such as MSH6, MSH2, or L1CAM. These results are expected to advance the quest for biomarkers to accurately assess the risk of EC patients. more...
- Published
- 2021
- Full Text
- View/download PDF
77. Cystathionine-γ-lyase drives antioxidant defense in cysteine-restricted IDH1-mutant astrocytomas.
- Author
-
Cano-Galiano A, Oudin A, Fack F, Allega MF, Sumpton D, Martinez-Garcia E, Dittmar G, Hau AC, De Falco A, Herold-Mende C, Bjerkvig R, Meiser J, Tardito S, and Niclou SP
- Abstract
Background: Mutations in isocitrate dehydrogenase 1 or 2 ( IDH1/2 ) define glioma subtypes and are considered primary events in gliomagenesis, impacting tumor epigenetics and metabolism. IDH enzyme activity is crucial for the generation of reducing potential in normal cells, yet the impact of the mutation on the cellular antioxidant system in glioma is not understood. The aim of this study was to determine how glutathione (GSH), the main antioxidant in the brain, is maintained in IDH1-mutant gliomas, despite an altered NADPH/NADP balance., Methods: Proteomics, metabolomics, metabolic tracer studies, genetic silencing, and drug targeting approaches in vitro and in vivo were applied. Analyses were done in clinical specimen of different glioma subtypes, in glioma patient-derived cell lines carrying the endogenous IDH1 mutation and corresponding orthotopic xenografts in mice., Results: We find that cystathionine-γ-lyase (CSE), the enzyme responsible for cysteine production upstream of GSH biosynthesis, is specifically upregulated in IDH1-mutant astrocytomas. CSE inhibition sensitized these cells to cysteine depletion, an effect not observed in IDH1 wild-type gliomas. This correlated with an increase in reactive oxygen species and reduced GSH synthesis. Propargylglycine (PAG), a brain-penetrant drug specifically targeting CSE, led to delayed tumor growth in mice., Conclusions: W e show that IDH1-mutant astrocytic gliomas critically rely on NADPH-independent de novo GSH synthesis via CSE to maintain the antioxidant defense, which highlights a novel metabolic vulnerability that may be therapeutically exploited., (© The Author(s) 2021. Published by Oxford University Press, the Society for Neuro-Oncology and the European Association of Neuro-Oncology.) more...
- Published
- 2021
- Full Text
- View/download PDF
78. Prognostic Biomarkers in Endometrial Cancer: A Systematic Review and Meta-Analysis.
- Author
-
Coll-de la Rubia E, Martinez-Garcia E, Dittmar G, Gil-Moreno A, Cabrera S, and Colas E
- Abstract
Endometrial cancer (EC) is the sixth most common cancer in women worldwide and its mortality is directly associated with the presence of poor prognostic factors driving tumor recurrence. Stratification systems are based on few molecular, and mostly clinical and pathological parameters, but these systems remain inaccurate. Therefore, identifying prognostic EC biomarkers is crucial for improving risk assessment pre- and postoperatively and to guide treatment decisions. This systematic review gathers all protein biomarkers associated with clinical prognostic factors of EC, recurrence and survival. Relevant studies were identified by searching the PubMed database from 1991 to February 2020. A total number of 398 studies matched our criteria, which compiled 255 proteins associated with the prognosis of EC. MUC16, ESR1, PGR, TP53, WFDC2, MKI67, ERBB2, L1CAM, CDH1, PTEN and MMR proteins are the most validated biomarkers. On the basis of our meta-analysis ESR1, TP53 and WFDC2 showed potential usefulness for predicting overall survival in EC. Limitations of the published studies in terms of appropriate study design, lack of high-throughput measurements, and statistical deficiencies are highlighted, and new approaches and perspectives for the identification and validation of clinically valuable EC prognostic biomarkers are discussed. more...
- Published
- 2020
- Full Text
- View/download PDF
79. ALCAM shedding at the invasive front of the tumor is a marker of myometrial infiltration and promotes invasion in endometrioid endometrial cancer.
- Author
-
Devis L, Martinez-Garcia E, Moiola CP, Quiles MT, Arbos MA, Stirbat TV, Brochard-Wyart F, García Á, Alonso-Alconada L, Abal M, Diaz-Feijoo B, Thomas W, Dufour S, Mancebo G, Alameda F, Reventos J, Gil-Moreno A, and Colas E more...
- Abstract
Endometrial cancer (EC) is the sixth deadliest cancer in women. The depth of myometrial invasion is one of the most important prognostic factors, being directly associated with tumor recurrence and mortality. In this study, ALCAM, a previously described marker of EC recurrence, was studied by immunohistochemistry at the superficial and the invasive tumor areas from 116 EC patients with different degree of myometrial invasion and related to a set of relevant epithelial and mesenchymal markers. ALCAM expression presented a heterogeneous functionality depending on its localization, it correlated with epithelial markers (E-cadherin/β-catenin) at the superficial area, and with mesenchymal markers at the invasive front (COX-2, SNAIL, ETV5, and MMP-9). At the invasive front, ALCAM-negativity was an independent marker of myometrial invasion. This negativity, together with an increase of soluble ALCAM in uterine aspirates from patients with an invasive EC, and its positive correlation with MMP-9 levels, suggested that ALCAM shedding by MMP-9 occurs at the invasive front. In vivo and in vitro models of invasive EC were generated by ETV5-overexpression. In those, we demonstrated that ALCAM shedding was related to a more invasive pattern and that full-ALCAM recovery reverted most of the ETV5-cells mesenchymal abilities, partially through a p-ERK dependent-manner., Competing Interests: CONFLICTS OF INTEREST The authors declare no conflicts of interest. more...
- Published
- 2018
- Full Text
- View/download PDF
80. Advances in endometrial cancer protein biomarkers for use in the clinic.
- Author
-
Martinez-Garcia E, Lopez-Gil C, Campoy I, Vallve J, Coll E, Cabrera S, Ramon Y Cajal S, Matias-Guiu X, Van Oostrum J, Reventos J, Gil-Moreno A, and Colas E
- Subjects
- Endometrial Neoplasms metabolism, Female, Humans, Mass Spectrometry methods, Proteins analysis, Biomarkers, Tumor analysis, Endometrial Neoplasms diagnosis, Proteomics
- Abstract
Introduction: Endometrial cancer (EC) is the fourth most common cancer in women in developed countries. The identification of sensitive and specific biomarkers to improve early detection of EC is crucial for an appropriate management of this disease, in which 30% of patients are diagnosed only at advanced stages, which is associated with high levels of morbidity and mortality. Despite major efforts and investments made to identify EC biomarkers, no protein has yet reached the stage of clinical application. Areas covered: This review gathers the numerous candidate biomarkers for EC diagnosis proposed in proteomic studies published from 1978 to 2017. Additionally, we summarize limitations associated with the proteomic technologies and study designs employed in those articles. Finally, we address new perspectives in EC biomarker research, including the comprehensive knowledge of previously suggested candidate biomarkers in conjunction with novel mass spectrometry-based proteomic technologies with enhanced sensitivity and specificity not yet applied to EC studies and a directed clinical perspective in the study design. Expert commentary: These ingredients could be the recipe to accelerate the application of protein biomarkers in the clinic. more...
- Published
- 2018
- Full Text
- View/download PDF
81. Targeted Proteomics Identifies Proteomic Signatures in Liquid Biopsies of the Endometrium to Diagnose Endometrial Cancer and Assist in the Prediction of the Optimal Surgical Treatment.
- Author
-
Martinez-Garcia E, Lesur A, Devis L, Cabrera S, Matias-Guiu X, Hirschfeld M, Asberger J, van Oostrum J, Casares de Cal MLÁ, Gómez-Tato A, Reventos J, Domon B, Colas E, and Gil-Moreno A
- Subjects
- Adult, Aged, Aged, 80 and over, Biomarkers, Tumor metabolism, Body Fluids metabolism, Endometrial Neoplasms genetics, Endometrial Neoplasms pathology, Endometrial Neoplasms surgery, Endometrium metabolism, Endometrium pathology, Female, Humans, Mass Spectrometry, Middle Aged, Prognosis, Proteome genetics, Uterine Neoplasms diagnosis, Uterine Neoplasms genetics, Uterine Neoplasms pathology, Uterine Neoplasms surgery, Biomarkers, Tumor genetics, Endometrial Neoplasms diagnosis, Karyopherins genetics, Liquid Biopsy methods, Microfilament Proteins genetics, Nuclear Proteins genetics, alpha Catenin genetics
- Abstract
Purpose: Endometrial cancer (EC) diagnosis relies on the observation of tumor cells in endometrial biopsies obtained by aspiration (i.e., uterine aspirates), but it is associated with 22% undiagnosed patients and up to 50% of incorrectly assigned EC histotype and grade. We aimed to identify biomarker signatures in the fluid fraction of these biopsies to overcome these limitations. Experimental Design: The levels of 52 proteins were measured in the fluid fraction of uterine aspirates from 116 patients by LC-PRM, the latest generation of targeted mass-spectrometry acquisition. A logistic regression model was used to assess the power of protein panels to differentiate between EC and non-EC patients and between EC histologic subtypes. The robustness of the panels was assessed by the "leave-one-out" cross-validation procedure performed within the same cohort of patients and an independent cohort of 38 patients. Results: The levels of 28 proteins were significantly higher in patients with EC ( n = 69) compared with controls ( n = 47). The combination of MMP9 and KPYM exhibited 94% sensitivity and 87% specificity for detecting EC cases. This panel perfectly complemented the standard diagnosis, achieving 100% of correct diagnosis in this dataset. Nine proteins were significantly increased in endometrioid EC ( n = 49) compared with serous EC ( n = 20). The combination of CTNB1, XPO2, and CAPG achieved 95% sensitivity and 96% specificity for the discrimination of these subtypes. Conclusions: We developed two uterine aspirate-based signatures to diagnose EC and classify tumors in the most prevalent histologic subtypes. This will improve diagnosis and assist in the prediction of the optimal surgical treatment. Clin Cancer Res; 23(21); 6458-67. ©2017 AACR ., (©2017 American Association for Cancer Research.) more...
- Published
- 2017
- Full Text
- View/download PDF
82. Activated leukocyte cell adhesion molecule (ALCAM) is a marker of recurrence and promotes cell migration, invasion, and metastasis in early-stage endometrioid endometrial cancer.
- Author
-
Devis L, Moiola CP, Masia N, Martinez-Garcia E, Santacana M, Stirbat TV, Brochard-Wyart F, García Á, Alameda F, Cabrera S, Palacios J, Moreno-Bueno G, Abal M, Thomas W, Dufour S, Matias-Guiu X, Santamaria A, Reventos J, Gil-Moreno A, and Colas E more...
- Subjects
- Aged, Animals, Antigens, CD metabolism, Biomarkers, Tumor metabolism, Carcinoma, Endometrioid diagnosis, Carcinoma, Endometrioid pathology, Cell Adhesion Molecules, Neuronal metabolism, Cell Movement, Endometrial Neoplasms diagnosis, Endometrial Neoplasms pathology, Female, Fetal Proteins metabolism, Filamins genetics, Filamins metabolism, Humans, Laminin genetics, Laminin metabolism, Mice, Mice, Nude, Middle Aged, Neoplasm Invasiveness, Neoplasm Metastasis, Prognosis, Retrospective Studies, Signal Transduction, Thioredoxin Reductase 1 genetics, Thioredoxin Reductase 1 metabolism, Antigens, CD genetics, Biomarkers, Tumor genetics, Carcinoma, Endometrioid genetics, Cell Adhesion Molecules, Neuronal genetics, Endometrial Neoplasms genetics, Fetal Proteins genetics, Gene Expression Regulation, Neoplastic
- Abstract
Endometrial cancer is the most common gynaecological cancer in western countries, being the most common subtype of endometrioid tumours. Most patients are diagnosed at an early stage and present an excellent prognosis. However, a number of those continue to suffer recurrence, without means of identification by risk classification systems. Thus, finding a reliable marker to predict recurrence becomes an important unmet clinical issue. ALCAM is a cell-cell adhesion molecule and member of the immunoglobulin superfamily that has been associated with the genesis of many cancers. Here, we first determined the value of ALCAM as a marker of recurrence in endometrioid endometrial cancer by conducting a retrospective multicentre study of 174 primary tumours. In early-stage patients (N = 134), recurrence-free survival was poorer in patients with ALCAM-positive compared to ALCAM-negative tumours (HR 4.237; 95% CI 1.01-17.76). This difference was more significant in patients with early-stage moderately-poorly differentiated tumours (HR 9.259; 95% CI 2.12-53.47). In multivariate analysis, ALCAM positivity was an independent prognostic factor in early-stage disease (HR 6.027; 95% CI 1.41-25.74). Then we demonstrated in vitro a role for ALCAM in cell migration and invasion by using a loss-of-function model in two endometrial cancer cell lines. ALCAM depletion resulted in a reduced primary tumour size and reduced metastatic local spread in an orthotopic murine model. Gene expression analysis of ALCAM-depleted cell lines pointed to motility, invasiveness, cellular assembly, and organization as the most deregulated functions. Finally, we assessed some of the downstream effector genes that are involved in ALCAM-mediated cell migration; specifically FLNB, TXNRD1, and LAMC2 were validated at the mRNA and protein level. In conclusion, our results highlight the potential of ALCAM as a recurrent biomarker in early-stage endometrioid endometrial cancer and point to ALCAM as an important molecule in endometrial cancer dissemination by regulating cell migration, invasion, and metastasis. Copyright © 2016 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd., (Copyright © 2016 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.) more...
- Published
- 2017
- Full Text
- View/download PDF
83. Association between bone turnover markers, clinical variables, spinal syndesmophytes and bone mineral density in Mexican patients with ankylosing spondylitis.
- Author
-
Gamez-Nava JI, de la Cerda-Trujillo LF, Vazquez-Villegas ML, Cons-Molina F, Alcaraz-Lopez MF, Zavaleta-Muñiz SA, Rocha-Muñoz AD, Martinez-Garcia EA, Corona-Sanchez EG, Salazar-Paramo M, Fajardo-Robledo NS, Olivas-Flores EM, Cardona-Muñoz EG, and Gonzalez-Lopez L more...
- Subjects
- Adult, Biomarkers blood, Case-Control Studies, Female, Humans, Male, Middle Aged, Radiography, Spondylitis, Ankylosing blood, Spondylitis, Ankylosing diagnostic imaging, Young Adult, Bone Density, Bone Remodeling, Cervical Vertebrae diagnostic imaging, Lumbar Vertebrae diagnostic imaging, Spondylitis, Ankylosing physiopathology
- Abstract
Objectives: To compare bone turnover marker (BTM) levels and bone mineral density (BMD) between patients with ankylosing spondylitis (AS) and healthy controls (HC) and to evaluate, in AS, the association between BTM levels and clinical variables, spinal syndesmophytes, and BMD using multivariate analysis., Method: Seventy-eight AS patients were compared with 58 HC matched by gender. Spinal syndesmophytes in AS and other characteristics were assessed. C-terminal telopeptide fragments of type I collagen (CTX), bone-specific alkaline phosphatase (BAP), osteocalcin (OC) serum levels, and BMD of the lumbar spine, femoral neck, and forearm were evaluated., Results: AS males and females had lower BAP levels than their respective HC (p < 0.001 and p = 0.001). AS patients with bridging syndesmophytes had higher OC levels than AS patients either with non-bridging syndesmophytes (p = 0.001) or without spinal syndesmophytes (p < 0.001). OC and CTX levels correlated significantly with the modified Stoke Ankylosing Spondylitis Spinal Score (mSASSS). In the multivariate linear regression adjusted by age, gender, the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), BMD in the lumbar spine, and C-reactive protein (CRP), we observed an association between BAP levels and anti-tumour necrosis factor (anti-TNF) use (p = 0.05) whereas OC levels were associated with mSASSS (p < 0.001) and anti-TNF use (p = 0.05), and CTX levels were exclusively associated with mSASSS (p = 0.03). In the logistic regression analysis, only OC levels were associated with the presence of syndesmophytes in AS [odds ratio (OR) 2.42, 95% confidence interval (CI) 1.19-5.75]., Conclusions: We observed an increase in OC levels in AS patients with syndesmophytes. BTM levels were associated with the severity of spinal damage. Future longitudinal studies should evaluate whether these BTMs should be included as tools to determine the prognosis and progression of spinal damage. more...
- Published
- 2016
- Full Text
- View/download PDF
84. Development of a sequential workflow based on LC-PRM for the verification of endometrial cancer protein biomarkers in uterine aspirate samples.
- Author
-
Martinez-Garcia E, Lesur A, Devis L, Campos A, Cabrera S, van Oostrum J, Matias-Guiu X, Gil-Moreno A, Reventos J, Colas E, and Domon B
- Subjects
- Base Sequence, Biopsy, Needle, Endometrial Neoplasms pathology, Endometrium pathology, Female, Humans, Proteomics methods, Reproducibility of Results, Uterus pathology, Biomarkers, Tumor metabolism, Endometrial Neoplasms metabolism, Endometrium metabolism, Mass Spectrometry methods, Research Design
- Abstract
About 30% of endometrial cancer (EC) patients are diagnosed at an advanced stage of the disease, which is associated with a drastic decrease in the 5-year survival rate. The identification of biomarkers in uterine aspirate samples, which are collected by a minimally invasive procedure, would improve early diagnosis of EC. We present a sequential workflow to select from a list of potential EC biomarkers, those which are the most promising to enter a validation study. After the elimination of confounding contributions by residual blood proteins, 52 potential biomarkers were analyzed in uterine aspirates from 20 EC patients and 18 non-EC controls by a high-resolution accurate mass spectrometer operated in parallel reaction monitoring mode. The differential abundance of 26 biomarkers was observed, and among them ten proteins showed a high sensitivity and specificity (AUC > 0.9). The study demonstrates that uterine aspirates are valuable samples for EC protein biomarkers screening. It also illustrates the importance of a biomarker verification phase to fill the gap between discovery and validation studies and highlights the benefits of high resolution mass spectrometry for this purpose. The proteins verified in this study have an increased likelihood to become a clinical assay after a subsequent validation phase., Competing Interests: The authors declare no conflicts of interest. more...
- Published
- 2016
- Full Text
- View/download PDF
85. Correction: Biofilm Formation As a Response to Ecological Competition.
- Author
-
Oliveira NM, Martinez-Garcia E, Xavier J, Durham WM, Kolter R, Kim W, and Foster KR
- Published
- 2015
- Full Text
- View/download PDF
86. Biofilm Formation As a Response to Ecological Competition.
- Author
-
Oliveira NM, Martinez-Garcia E, Xavier J, Durham WM, Kolter R, Kim W, and Foster KR
- Subjects
- Anti-Bacterial Agents, Biofilms drug effects, Coculture Techniques, Microfluidics, Antibiosis, Biofilms growth & development, Pseudomonas aeruginosa growth & development, Pyocins pharmacology
- Abstract
Bacteria form dense surface-associated communities known as biofilms that are central to their persistence and how they affect us. Biofilm formation is commonly viewed as a cooperative enterprise, where strains and species work together for a common goal. Here we explore an alternative model: biofilm formation is a response to ecological competition. We co-cultured a diverse collection of natural isolates of the opportunistic pathogen Pseudomonas aeruginosa and studied the effect on biofilm formation. We show that strain mixing reliably increases biofilm formation compared to unmixed conditions. Importantly, strain mixing leads to strong competition: one strain dominates and largely excludes the other from the biofilm. Furthermore, we show that pyocins, narrow-spectrum antibiotics made by other P. aeruginosa strains, can stimulate biofilm formation by increasing the attachment of cells. Side-by-side comparisons using microfluidic assays suggest that the increase in biofilm occurs due to a general response to cellular damage: a comparable biofilm response occurs for pyocins that disrupt membranes as for commercial antibiotics that damage DNA, inhibit protein synthesis or transcription. Our data show that bacteria increase biofilm formation in response to ecological competition that is detected by antibiotic stress. This is inconsistent with the idea that sub-lethal concentrations of antibiotics are cooperative signals that coordinate microbial communities, as is often concluded. Instead, our work is consistent with competition sensing where low-levels of antibiotics are used to detect and respond to the competing genotypes that produce them. more...
- Published
- 2015
- Full Text
- View/download PDF
87. Nidogen 1 and Nuclear Protein 1: novel targets of ETV5 transcription factor involved in endometrial cancer invasion.
- Author
-
Pedrola N, Devis L, Llauradó M, Campoy I, Martinez-Garcia E, Garcia M, Muinelo-Romay L, Alonso-Alconada L, Abal M, Alameda F, Mancebo G, Carreras R, Castellví J, Cabrera S, Gil-Moreno A, Matias-Guiu X, Iovanna JL, Colas E, Reventós J, and Ruiz A more...
- Subjects
- Animals, Apoptosis, Base Sequence, Basic Helix-Loop-Helix Transcription Factors genetics, Blotting, Western, Chromatin Immunoprecipitation, DNA-Binding Proteins genetics, Endometrial Neoplasms genetics, Endometrial Neoplasms metabolism, Female, Humans, Immunoenzyme Techniques, Luciferases metabolism, Membrane Glycoproteins genetics, Mice, Mice, Nude, Molecular Sequence Data, Neoplasm Invasiveness, Neoplasm Proteins genetics, RNA, Messenger genetics, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Transcription Factors genetics, Tumor Cells, Cultured, Xenograft Model Antitumor Assays, Basic Helix-Loop-Helix Transcription Factors metabolism, Cell Movement, Cell Proliferation, DNA-Binding Proteins metabolism, Endometrial Neoplasms pathology, Gene Expression Regulation, Neoplastic, Membrane Glycoproteins metabolism, Neoplasm Proteins metabolism, Transcription Factors metabolism
- Abstract
Endometrial cancer is the most frequent malignancy of the female genital tract in western countries. Our group has previously characterized the upregulation of the transcription factor ETV5 in endometrial cancer with a specific and significant increase in those tumor stages associated with myometrial invasion. We have shown that ETV5 overexpression in Hec1A endometrial cancer cells induces epithelial to mesenchymal transition resulting in the acquisition of migratory and invasive capabilities. In the present work, we have identified Nidogen 1 (NID1) and Nuclear Protein 1 (NUPR1) as direct transcriptional targets of ETV5 in endometrial cancer cells. Inhibition of NID1 and NUPR1 in ETV5 overexpressing cells reduced cell migration and invasion in vitro and reduced tumor growth and dissemination in an orthotopic endometrial cancer model. Importantly, we confirmed a significant increase of NUPR1 and NID1 protein expression in the invasion front of the tumor compared to their paired superficial zone, concomitant to ETV5 overexpression. Altogether, we conclude that NID1 and NUPR1 are novel targets of ETV5 and are actively cooperating with ETV5 at the invasion front of the tumor in the acquisition of an invasive phenotype to jointly drive endometrial cancer invasion. more...
- Published
- 2015
- Full Text
- View/download PDF
88. Benthic community recovery from brine impact after the implementation of mitigation measures.
- Author
-
Del-Pilar-Ruso Y, Martinez-Garcia E, Giménez-Casalduero F, Loya-Fernández A, Ferrero-Vicente LM, Marco-Méndez C, de-la-Ossa-Carretero JA, and Sánchez-Lizaso JL
- Subjects
- Animals, Environmental Monitoring, Spain, Environmental Restoration and Remediation, Polychaeta drug effects, Seawater analysis, Water Pollutants, Chemical analysis, Water Purification
- Abstract
In many regions, seawater desalination is a growing industry that has its impact on benthic communities. This study analyses the effect on benthic communities of a mitigation measure applied to a brine discharge, using polychaete assemblages as indicator. An eight-year study was conducted at San Pedro del Pinatar (SE Spain) establishing a grid of 12 sites at a depth range of 29-38 m during autumn. Brine discharge started in 2006 and produced a significant decrease in abundance, richness and diversity of polychaete families at the location closest to the discharge, where salinity reached 49. In 2010, a diffuser was deployed at the end of the pipeline in order to increase the mixing, to reduce the impact on benthic communities. After implementation of this mitigation measure, the salinity measured close to discharge was less than 38.5 and a significant recovery in polychaete richness and diversity was detected, to levels similar to those before the discharge. A less evident recovery in abundance was also observed, probably due to different recovery rates of polychaete families. Some families like Paraonidae and Magelonidae were more tolerant to this impact. Others like Syllidae and Capitellidae recovered quickly, although still affected by the discharge, while some families such as Sabellidae and Cirratulidae appeared to recover more slowly., (Copyright © 2014 Elsevier Ltd. All rights reserved.) more...
- Published
- 2015
- Full Text
- View/download PDF
89. Histone methyltransferase MMSET/NSD2 alters EZH2 binding and reprograms the myeloma epigenome through global and focal changes in H3K36 and H3K27 methylation.
- Author
-
Popovic R, Martinez-Garcia E, Giannopoulou EG, Zhang Q, Zhang Q, Ezponda T, Shah MY, Zheng Y, Will CM, Small EC, Hua Y, Bulic M, Jiang Y, Carrara M, Calogero RA, Kath WL, Kelleher NL, Wang JP, Elemento O, and Licht JD more...
- Subjects
- Animals, Cell Line, Cell Transformation, Neoplastic genetics, Chromatin genetics, Female, HEK293 Cells, Histone-Lysine N-Methyltransferase genetics, Histones genetics, Humans, Lysine genetics, Mice, Mice, Inbred C57BL, Multiple Myeloma metabolism, Polycomb Repressive Complex 2 genetics, RNA, Small Interfering genetics, DNA Methylation genetics, Epigenesis, Genetic genetics, Histone-Lysine N-Methyltransferase metabolism, Histones metabolism, Multiple Myeloma genetics, Polycomb Repressive Complex 2 metabolism, Protein Binding genetics
- Abstract
Overexpression of the histone methyltransferase MMSET in t(4;14)+ multiple myeloma patients is believed to be the driving factor in the pathogenesis of this subtype of myeloma. MMSET catalyzes dimethylation of lysine 36 on histone H3 (H3K36me2), and its overexpression causes a global increase in H3K36me2, redistributing this mark in a broad, elevated level across the genome. Here, we demonstrate that an increased level of MMSET also induces a global reduction of lysine 27 trimethylation on histone H3 (H3K27me3). Despite the net decrease in H3K27 methylation, specific genomic loci exhibit enhanced recruitment of the EZH2 histone methyltransferase and become hypermethylated on this residue. These effects likely contribute to the myeloma phenotype since MMSET-overexpressing cells displayed increased sensitivity to EZH2 inhibition. Furthermore, we demonstrate that such MMSET-mediated epigenetic changes require a number of functional domains within the protein, including PHD domains that mediate MMSET recruitment to chromatin. In vivo, targeting of MMSET by an inducible shRNA reversed histone methylation changes and led to regression of established tumors in athymic mice. Together, our work elucidates previously unrecognized interplay between MMSET and EZH2 in myeloma oncogenesis and identifies domains to be considered when designing inhibitors of MMSET function. more...
- Published
- 2014
- Full Text
- View/download PDF
90. The proto-oncometabolite fumarate binds glutathione to amplify ROS-dependent signaling.
- Author
-
Sullivan LB, Martinez-Garcia E, Nguyen H, Mullen AR, Dufour E, Sudarshan S, Licht JD, Deberardinis RJ, and Chandel NS
- Subjects
- Carcinoma, Renal Cell pathology, Chromatography, Liquid, Fumarate Hydratase antagonists & inhibitors, Fumarate Hydratase genetics, Fumarate Hydratase metabolism, Glutathione Reductase metabolism, Histone Demethylases metabolism, Histones metabolism, Humans, Hypoxia-Inducible Factor 1, alpha Subunit antagonists & inhibitors, Hypoxia-Inducible Factor 1, alpha Subunit genetics, Hypoxia-Inducible Factor 1, alpha Subunit metabolism, Immunoblotting, Kidney Neoplasms pathology, NADP metabolism, NF-E2-Related Factor 2 antagonists & inhibitors, NF-E2-Related Factor 2 genetics, NF-E2-Related Factor 2 metabolism, Oxygen Consumption, RNA, Small Interfering genetics, Signal Transduction, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Tumor Cells, Cultured, Carcinoma, Renal Cell metabolism, Fumarates metabolism, Glutathione metabolism, Kidney Neoplasms metabolism, Reactive Oxygen Species metabolism
- Abstract
The tricarboxylic acid cycle enzyme fumarate hydratase (FH) has been identified as a tumor suppressor in a subset of human renal cell carcinomas. Human FH-deficient cancer cells display high fumarate concentration and ROS levels along with activation of HIF-1. The underlying mechanisms by which FH loss increases ROS and HIF-1 are not fully understood. Here, we report that glutamine-dependent oxidative citric acid cycle metabolism is required to generate fumarate and increase ROS and HIF-1 levels. Accumulated fumarate directly bonds the antioxidant glutathione in vitro and in vivo to produce the metabolite succinated glutathione (GSF). GSF acts as an alternative substrate to glutathione reductase to decrease NADPH levels and enhance mitochondrial ROS and HIF-1 activation. Increased ROS also correlates with hypermethylation of histones in these cells. Thus, fumarate serves as a proto-oncometabolite by binding to glutathione which results in the accumulation of ROS., (Copyright © 2013 Elsevier Inc. All rights reserved.) more...
- Published
- 2013
- Full Text
- View/download PDF
91. EZH2 is required for germinal center formation and somatic EZH2 mutations promote lymphoid transformation.
- Author
-
Béguelin W, Popovic R, Teater M, Jiang Y, Bunting KL, Rosen M, Shen H, Yang SN, Wang L, Ezponda T, Martinez-Garcia E, Zhang H, Zheng Y, Verma SK, McCabe MT, Ott HM, Van Aller GS, Kruger RG, Liu Y, McHugh CF, Scott DW, Chung YR, Kelleher N, Shaknovich R, Creasy CL, Gascoyne RD, Wong KK, Cerchietti L, Levine RL, Abdel-Wahab O, Licht JD, Elemento O, and Melnick AM more...
- Subjects
- Animals, Cell Differentiation, Cell Proliferation, Enhancer of Zeste Homolog 2 Protein, Gene Deletion, Gene Expression Regulation, Neoplastic, Germinal Center drug effects, Histones metabolism, Methylation, Mice, Polycomb Repressive Complex 2 genetics, Polycomb Repressive Complex 2 metabolism, Promoter Regions, Genetic, Proto-Oncogene Proteins c-bcl-2 metabolism, Proto-Oncogene Proteins c-bcl-2 physiology, B-Lymphocytes metabolism, Cell Transformation, Neoplastic genetics, Germinal Center metabolism, Mutation, Polycomb Repressive Complex 2 physiology
- Abstract
The EZH2 histone methyltransferase is highly expressed in germinal center (GC) B cells and targeted by somatic mutations in B cell lymphomas. Here, we find that EZH2 deletion or pharmacologic inhibition suppresses GC formation and functions. EZH2 represses proliferation checkpoint genes and helps establish bivalent chromatin domains at key regulatory loci to transiently suppress GC B cell differentiation. Somatic mutations reinforce these physiological effects through enhanced silencing of EZH2 targets. Conditional expression of mutant EZH2 in mice induces GC hyperplasia and accelerated lymphomagenesis in cooperation with BCL2. GC B cell (GCB)-type diffuse large B cell lymphomas (DLBCLs) are mostly addicted to EZH2 but not the more differentiated activated B cell (ABC)-type DLBCLs, thus clarifying the therapeutic scope of EZH2 targeting., (Copyright © 2013 Elsevier Inc. All rights reserved.) more...
- Published
- 2013
- Full Text
- View/download PDF
92. A meta-analysis approach to the effects of fish farming on soft bottom polychaeta assemblages in temperate regions.
- Author
-
Martinez-Garcia E, Sanchez-Jerez P, Aguado-Giménez F, Ávila P, Guerrero A, Sánchez-Lizaso JL, Fernandez-Gonzalez V, González N, Gairin JI, Carballeira C, García-García B, Carreras J, Macías JC, Carballeira A, and Collado C more...
- Subjects
- Animals, Biodiversity, Environmental Monitoring, Fishes, Polychaeta growth & development, Spain, Aquaculture, Geologic Sediments chemistry, Meta-Analysis as Topic, Polychaeta classification, Water Pollutants analysis, Water Pollution statistics & numerical data
- Abstract
Marine fish farms could cause environmental disturbances on the sediment due to uneaten food and fish faeces that impact the marine benthos. Polychaete assemblages are considered good indicators of environmental perturbations. The present study aimed to establish groups of polychaetes as potential indicators of fish farm pollution. This study was carried out in ten fish farms along the Spanish coast. Changes in polychaete assemblage were analyzed with meta-analysis and multivariate techniques. Abundance, richness and diversity showed significant decreases under fish farm conditions. Distribution patterns of polychaetes responded to combinations of physicochemical variables. The main ones are sulfide concentration, silt and clays percentage, and stable nitrogen isotope ratio. The results showed that some families are tolerant, Capitellidae, Dorvilleidae, Glyceridae, Nereididae, Oweniidae and Spionidae; while others are sensitive to fish farm pollution, Magelonidae, Maldanidae, Nephtyidae, Onuphidae, Paralacydoniidae, Paraonide, Sabellidae and also Cirratulidae in spite of being reported as a tolerant family., (Copyright © 2013 Elsevier Ltd. All rights reserved.) more...
- Published
- 2013
- Full Text
- View/download PDF
93. Total kinetic analysis reveals how combinatorial methylation patterns are established on lysines 27 and 36 of histone H3.
- Author
-
Zheng Y, Sweet SM, Popovic R, Martinez-Garcia E, Tipton JD, Thomas PM, Licht JD, and Kelleher NL
- Subjects
- Biochemistry methods, Cell Line, Combinatorial Chemistry Techniques, Epigenomics, Histone-Lysine N-Methyltransferase genetics, Humans, Kinetics, Mass Spectrometry methods, Methylation, Methyltransferases chemistry, Repressor Proteins genetics, Histone-Lysine N-Methyltransferase chemistry, Histones chemistry, Lysine chemistry, Repressor Proteins chemistry
- Abstract
We have developed a targeted method to quantify all combinations of methylation on an H3 peptide containing lysines 27 and 36 (H3K27-K36). By using stable isotopes that separately label the histone backbone and its methylations, we tracked the rates of methylation and demethylation in myeloma cells expressing high vs. low levels of the methyltransferase MMSET/WHSC1/NSD2. Following quantification of 99 labeled H3K27-K36 methylation states across time, a kinetic model converged to yield 44 effective rate constants qualifying each methylation and demethylation step as a function of the methylation state on the neighboring lysine. We call this approach MS-based measurement and modeling of histone methylation kinetics (M4K). M4K revealed that, when dimethylation states are reached on H3K27 or H3K36, rates of further methylation on the other site are reduced as much as 100-fold. Overall, cells with high MMSET have as much as 33-fold increases in the effective rate constants for formation of H3K36 mono- and dimethylation. At H3K27, cells with high MMSET have elevated formation of K27me1, but even higher increases in the effective rate constants for its reversal by demethylation. These quantitative studies lay bare a bidirectional antagonism between H3K27 and H3K36 that controls the writing and erasing of these methylation marks. Additionally, the integrated kinetic model was used to correctly predict observed abundances of H3K27-K36 methylation states within 5% of that actually established in perturbed cells. Such predictive power for how histone methylations are established should have major value as this family of methyltransferases matures as drug targets. more...
- Published
- 2012
- Full Text
- View/download PDF
94. The MMSET histone methyl transferase switches global histone methylation and alters gene expression in t(4;14) multiple myeloma cells.
- Author
-
Martinez-Garcia E, Popovic R, Min DJ, Sweet SM, Thomas PM, Zamdborg L, Heffner A, Will C, Lamy L, Staudt LM, Levens DL, Kelleher NL, and Licht JD
- Subjects
- Apoptosis, Biomarkers, Tumor genetics, Biomarkers, Tumor metabolism, Blotting, Western, Cell Adhesion, Cell Cycle, Cell Movement, Cell Proliferation, Chromatin genetics, Chromatin Immunoprecipitation, Epigenomics, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Histone-Lysine N-Methyltransferase antagonists & inhibitors, Histone-Lysine N-Methyltransferase metabolism, Humans, Oligonucleotide Array Sequence Analysis, Protein Isoforms, RNA, Messenger genetics, RNA, Small Interfering genetics, Repressor Proteins antagonists & inhibitors, Repressor Proteins metabolism, Reverse Transcriptase Polymerase Chain Reaction, Tumor Cells, Cultured, Chromosomes, Human, Pair 14 genetics, Chromosomes, Human, Pair 4 genetics, DNA Methylation, Histone-Lysine N-Methyltransferase genetics, Histones genetics, Multiple Myeloma genetics, Repressor Proteins genetics, Translocation, Genetic genetics
- Abstract
The multiple myeloma SET domain (MMSET) protein is overexpressed in multiple myeloma (MM) patients with the translocation t(4;14). Although studies have shown the involvement of MMSET/Wolf-Hirschhorn syndrome candidate 1 in development, its mode of action in the pathogenesis of MM is largely unknown. We found that MMSET is a major regulator of chromatin structure and transcription in t(4;14) MM cells. High levels of MMSET correlate with an increase in lysine 36 methylation of histone H3 and a decrease in lysine 27 methylation across the genome, leading to a more open structural state of the chromatin. Loss of MMSET expression alters adhesion properties, suppresses growth, and induces apoptosis in MM cells. Consequently, genes affected by high levels of MMSET are implicated in the p53 pathway, cell cycle regulation, and integrin signaling. Regulation of many of these genes required functional histone methyl-transferase activity of MMSET. These results implicate MMSET as a major epigenetic regulator in t(4;14)+ MM. more...
- Published
- 2011
- Full Text
- View/download PDF
95. Deregulation of H3K27 methylation in cancer.
- Author
-
Martinez-Garcia E and Licht JD
- Subjects
- DNA-Binding Proteins chemistry, DNA-Binding Proteins genetics, Enhancer of Zeste Homolog 2 Protein, Germinal Center metabolism, Germinal Center pathology, Humans, Lymphoma, Large B-Cell, Diffuse genetics, Lymphoma, Large B-Cell, Diffuse pathology, Methylation, Mutation genetics, Polycomb Repressive Complex 2, Transcription Factors chemistry, Transcription Factors genetics, Histones metabolism, Lymphoma, Follicular genetics, Lysine metabolism
- Abstract
A new study now reports recurrent somatic mutation of EZH2, a histone methyltransferase that modifies H3K27, in diffuse large B-cell lymphoma (DLBCL). There is now evidence for both increased and decreased activity of enzymes controlling H3K27 methylation in cancer, suggesting that a precise balance of this methylation is critical for normal cell growth. more...
- Published
- 2010
- Full Text
- View/download PDF
96. Social evolution of spatial patterns in bacterial biofilms: when conflict drives disorder.
- Author
-
Xavier JB, Martinez-Garcia E, and Foster KR
- Subjects
- Biological Evolution, Biofilms growth & development, Models, Biological, Pseudomonas aeruginosa physiology
- Abstract
A key feature of biological systems is the emergence of higher-order structures from interacting units, such as the development of tissues from individual cells and the elaborate divisions of labor in insect societies. Little is known, however, of how evolutionary competition among individuals affects biological organization. Here we explore this link in bacterial biofilms, concrete systems that are well known for higher-order structures. We present a mechanistic model of cell growth at a surface, and we show that tension between growth and competition for nutrients can explain how empirically observed patterns emerge in biofilms. We then apply our model to evolutionary simulations and observe that the maintenance of patterns requires cooperation between cells. Specifically, when different genotypes meet and compete, natural selection favors energetically costly spreading strategies, like polymer secretion, that simultaneously reduce productivity and disrupt the spatial patterns. Our theory provides a formal link between higher-level patterning and the potential for evolutionary conflict by showing that both can arise from a single set of scale-dependent processes. Moreover, and contrary to previous theory, our analysis predicts an antagonistic relationship between evolutionary conflict and pattern formation: conflict drives disorder. more...
- Published
- 2009
- Full Text
- View/download PDF
97. Factors associated with delayed postsurgical voiding interval in ambulatory spinal anesthesia patients: a prospective cohort study in 3 types of surgery.
- Author
-
Linares Gil MJ, Esteve Gómez A, Blanco Vargas D, Martinez Garcia E, Daros FN, Tugas EI, Paises AA, and Pi-Siques F
- Subjects
- Adult, Ambulatory Surgical Procedures, Anesthetics, Local, Female, Humans, Lidocaine, Male, Middle Aged, Postoperative Complications, Prospective Studies, Risk Factors, Sex Factors, Anesthesia, Spinal adverse effects, Urinary Retention etiology
- Abstract
Background: Spinal anesthesia has been considered inappropriate for ambulatory surgery patients because of concern about voiding dysfunction. The purpose of this study was to analyze the relationship between voiding interval and type of surgery under spinal anesthesia with lidocaine and to identify other nonanesthetic risk factors for delayed voiding., Patients and Methods: A prospective study of 406 patients undergoing to ambulatory surgery under spinal anesthesia with lidocaine was performed. Voiding interval was defined as the time in minutes from the injection of local anesthetic to the patient's first spontaneous voiding. Univariate and multivariate linear regression models were constructed to identify risk factors associated with length of voiding interval., Results: A total of 187 patients underwent herniorrhaphy; 187 patients underwent lower limb surgery; and 32 patients went benign anorectal surgery. The mean +/- sd voiding interval was 230 +/- 50.5 minutes. Factors associated with length of voiding interval in the univariate analysis were sex, body mass index (BMI), type and duration of surgery, lidocaine dose, and volume of fluid administered. Factors that remained significant in the multivariate model were sex, BMI, lidocaine dose and type of surgery: spontaneous voiding came later after inguinal herniorrhaphy surgery than after lower-limb surgery (regression coefficient 20 minutes; 95% confidence interval 11.5-29.8). Multivariate models performed for each type of surgery separately identified sex and lidocaine dose as factors related to length of voiding interval in all types of surgery., Conclusions: A longer voiding interval was associated with inguinal herniorrhaphy, spinal lidocaine dose, and male sex. more...
- Published
- 2009
- Full Text
- View/download PDF
98. Horner's syndrome because of thoracic epidural analgesia with levobupivacaine in a pediatric patient.
- Author
-
Martinez-Garcia E, Del Rey P, Tormo C, de Santiago J, Porto R, and Serrano-Gonzalez A
- Subjects
- Adolescent, Bupivacaine adverse effects, Bupivacaine analogs & derivatives, Humans, Levobupivacaine, Male, Thoracic Vertebrae, Thoracoscopy, Analgesia, Epidural methods, Anesthetics, Local adverse effects, Horner Syndrome chemically induced
- Published
- 2008
- Full Text
- View/download PDF
99. Prophylactic recombinant factor VIIa administration to an infant with congenital systemic juvenile xanthogranuloma.
- Author
-
De Santiago J, Martinez-Garcia E, Giron J, Salcedo C, and Pérez-Gallardo A
- Subjects
- Female, Hemorrhage complications, Humans, Infant, Newborn, Recombinant Proteins administration & dosage, Recombinant Proteins therapeutic use, Xanthogranuloma, Juvenile complications, Factor VIIa administration & dosage, Factor VIIa therapeutic use, Hemorrhage prevention & control, Xanthogranuloma, Juvenile congenital, Xanthogranuloma, Juvenile surgery
- Abstract
We report the case of an infant affected with congenital systemic juvenile xanthogranuloma scheduled for central venous access system implantation (Port-a-Cath) and a liver and bone marrow biopsy. The patient had impaired liver function, thrombocytopenia, and coagulopathy which was refractory to daily fresh-frozen plasma and platelet infusions: 80 microg x kg(-1) dose(-1) of recombinant factor VIIa (rFVIIa) was administered i.v. every 2 h starting 30 min before the procedure and ending 6 h afterwards. Very minor bleeding was observed during the procedure. In conclusion, rFVIIa therapy was effective as prophylaxis for both invasive procedures in this patient with a coagulopathy which was refractory to other different therapies. more...
- Published
- 2006
- Full Text
- View/download PDF
100. Polymorphism in the yclC-rpoS region of enterobacteria.
- Author
-
Martinez-Garcia E, Tormo A, and Navarro-Lloréns JM
- Subjects
- Adenosine Triphosphatases genetics, Enterobacter cloacae genetics, Enterobacter cloacae pathogenicity, Enterobacteriaceae pathogenicity, Escherichia coli Proteins genetics, Humans, Molecular Sequence Data, MutS DNA Mismatch-Binding Protein, Sequence Analysis, DNA, Bacterial Proteins genetics, DNA-Binding Proteins, Enterobacteriaceae genetics, Polymorphism, Genetic, Sigma Factor genetics
- Abstract
The nucleotide sequence downstream from the rpoS gene in Enterobacter cloacae and Kluyvera cryocrescens contains the slyA-pad1-yclC genes. The DNA sequence of Enterobacter cloacae CETC960 shows a 2.6-kb insertion of unknown origin between rpoS and slyA. This 2.6-kb sequence has also been detected in species of Salmonella and in Pseudomonas aeruginosa, but not in the same location. This insertion has been detected in all the Enterobacter cloacae clinical strains studied although the size of the rpoS-yclC region was highly variable, possibly owing to the presence of insertions and/or deletions. The study of the rpoS-mutS region in other enterobacteria also showed variability in size. Our results support the idea of a variational hot spot in the rpoS-mutS region that could be related to pathogenesis and horizontal transfer. more...
- Published
- 2003
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.