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510 results on '"Marrazza, Giovanna"'

58. Myocardial Adaptation to Acute Oxygen Shortage : A Kinetic Analysis

Author

Merati, Giampiero, Allibardi, Sonia, Marrazza, Giovanna, Mascini, Marco, Samaja, Michele, Nemoto, Edwin M., editor, LaManna, Joseph C., editor, Cooper, Christopher, editor, Delpy, David, editor, Groebe, Karlfried, editor, Hunt, Thomas K., editor, Keipert, Peter, editor, Mayevsky, Avrahim, editor, Pittman, Roland N., editor, Rumsey, William L., editor, Vaupel, Peter, editor, and Wilson, David F., editor

Published
1997
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64. Portable optoelectronic system for monitoring enzymatic chemiluminescent reaction

Author

Costantini, F., Tiggelaar, R. M., Salvio, R., Nardecchia, M., Schlautmann, S., Manetti, C., Gardeniers, H. J.G.E., Caputo, D., Nascetti, A., de Cesare, G., Baldini, Francesco, Siciliano, Pietro, Rossi, Marco, Scalise, Lorenzo, Di Natale, Corrado, Ferrari, Vittorio, Militello, Valeria, Miolo, Giorgia, Andò, Bruno, Marletta, Vincenzo, Marrazza, Giovanna, Mesoscale Chemical Systems, and MESA+ Institute

Subjects
Amorphous silicon, Materials science, Microfluidics, Settore CHIM/07, Photodetector, anodic bonding, microfluidics, photosensors, 02 engineering and technology, Substrate (electronics), 01 natural sciences, law.invention, Horseradish peroxidase, chemistry.chemical_compound, Enzymatic reactions, law, Thin film, Microchannel, business.industry, 010401 analytical chemistry, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Photodiode, chemistry, Anodic bonding, Photosensors, Optoelectronics, 0210 nano-technology, business
Abstract

This work presents a portable lab-on-chip system, based on thin film electronic devices and an all-glass microfluidic network, for the real-time monitoring of enzymatic chemiluminescent reactions. The microfluidic network is patterned, through wet etching, in a 1.1 mm-thick glass substrate that is subsequently bonded to a 0.5 mm-thick glass substrate. The electronic devices are amorphous silicon p-i-n photosensors, deposited on the outer side of the thinner glass substrate. The photosensors, the microfluidic network and the electronic boards reading out the photodiodes’ current are enclosed in a small metallic box (\(10 \times 8 \times 15\,\mathrm{cm}^3\)) in order to ensure shielding from electromagnetic interferences. Preliminary tests have been performed immobilizing horseradish peroxidase on the inner wall of the microchannel as model enzyme for detecting hydrogen peroxide. Limits of detection and quantification equal to 18 and 60 \(\upmu \)M, respectively, have been found. These values are comparable to the best performances reported in literature for chemiluminescent-based optofluidic sensors.

Published
2019

70. Electrochemical imaging of localized sandwich dna hybridization using scanning electrochemical microscopy

Author

Palchetti, Ilaria, Laschi, Serena, Marrazza, Giovanna, and Mascini, Marco

Subjects
Nucleic acid hybridization -- Research, Electrochemical apparatus -- Usage, Scanning microscopy -- Methods, Chemistry
Abstract

Imaging of localized hybridization of nucleic acids immobilized on gold-DNA chip was performed by means of the feedback mode of scanning electrochemical microscopy (SECM). Thiol-tethered oligodeoxynucleotide (HS-ODN) probes, spotted on a gold surface, were hybridized with unmodified target sequence via sandwich hybridization with a biotinylated signaling probe. Spots where sequence-specific hybridization had occurred were developed by adding a streptavidin-alkaline phosphatase conjugate and biocatalyzed precipitation of an insoluble and insulating product. As a consequence, the surface conductivity of the spotted region of the chip where hybridization had taken place changed. These changes in conductivity were sensitively detected by the SECM tip. The proposed method allows imaging of a DNA array in a straightforward way. Analysis of real samples was also performed coupling this method with polymerase chain reaction. The imaging of 60 nM PCR amplicon (255 bp) was demonstrated.

Published
2007

83. Statement of Peer Review.

Author

Marrazza, Giovanna and Tombelli, Sara

Subjects
*PROFESSIONAL standards, *NUMBER systems, *INFORMATION processing, *CONFERENCES & conventions, *PUBLISHING
Published
2024
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