393 results on '"Marine-derived fungi"'
Search Results
52. Characterisation of a thermostable and proteolysis resistant phytase from Penicillium polonicum MF82 associated with the marine sponge Phorbas sp.
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Kalkan, Saban Orcun, Bozcal, Elif, Hames Tuna, Elif Esin, and Uzel, Atac
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SPONGES (Invertebrates) , *TRITON X-100 , *PENICILLIUM , *PHYTASES , *SOIL amendments , *ORGANIC solvents , *PROTEOLYTIC enzymes - Abstract
Phytases are widely used in human and animal nutrition, aquaculture, soil amendment, and in the production of lower myo-inositol phosphates for clinical purposes. Some of these applications, especially feed industry require robust enzymes. Since the marine environments are less studied compared to terrestrial environments, we evaluated the extracellular phytase activity of 110 marine derived filamentous fungal (MDFF) strains previously isolated from sponge and sediment samples of the Turkey. MDFF strains were qualitatively screened for their extracellular phytase activities and P. polonicum MF82 phytase was further characterized following partial purification. Optimum pH and temperature were determined as 5.5 and 60 °C respectively. A significant relative phytase activity was observed in the presence of urea and acetone. However, there was no phytase activity followed by the treatment with Triton X-100 and Tween 80. Characterization studies revealed that P. polonicum MF82 phytase has superior properties for industrial use including wide pH and temperature range for activity, high optimum activity temperature, high thermal and pH stability, resistance to many enzyme inhibitors including various heavy metals, denaturants, detergents, proteases and organic solvents. Phytase extracellularly produced by P. polonicum MF82 strain presents a good candidate for commercial applications. This study demonstrates that the MDFF strains are prolific sources for phytase and presents the first report about the production and characterization of the phytase from a marine-derived P. polonicum strain. [ABSTRACT FROM AUTHOR]
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- 2020
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53. New Tripeptide Derivatives Asterripeptides A–C from Vietnamese Mangrove-Derived Fungus Aspergillus terreus LM.5.2
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Elena V. Girich, Anton B. Rasin, Roman S. Popov, Ekaterina A. Yurchenko, Ekaterina A. Chingizova, Phan Thi Hoai Trinh, Ngo Thi Duy Ngoc, Mikhail V. Pivkin, Olesya I. Zhuravleva, and Anton N. Yurchenko
- Subjects
marine-derived fungi ,mangrove-derived fungi ,secondary metabolites ,diketopiperazines ,tripeptide derivatives ,cinnamic acid ,Biology (General) ,QH301-705.5 - Abstract
Three new tripeptide derivatives asterripeptides A–C (1–3) were isolated from Vietnamese mangrove-derived fungus Aspergillus terreus LM.5.2. Structures of isolated compounds were determined by a combination of NMR and ESIMS techniques. The absolute configurations of all stereocenters were determined using the Murfey’s method. The isolated compounds 1–3 contain a rare fungi cinnamic acid residue. The cytotoxicity of isolated compounds against several cancer cell lines and inhibition ability of sortase A from Staphylococcus aureus of asterripeptides A–C were investigated.
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- 2022
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54. Marine-Derived Indole Alkaloids and Their Biological and Pharmacological Activities
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Joko Tri Wibowo, Peni Ahmadi, Siti Irma Rahmawati, Asep Bayu, Masteria Yunovilsa Putra, and Anake Kijjoa
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indole alkaloids ,marine natural products ,bioactive compounds ,marine-derived fungi ,marine sponges ,Biology (General) ,QH301-705.5 - Abstract
Novel secondary metabolites from marine macroorganisms and marine-derived microorganisms have been intensively investigated in the last few decades. Several classes of compounds, especially indole alkaloids, have been a target for evaluating biological and pharmacological activities. As one of the most promising classes of compounds, indole alkaloids possess not only intriguing structural features but also a wide range of biological/pharmacological activities including antimicrobial, anti-inflammatory, anticancer, antidiabetic, and antiparasitic activities. This review reports the indole alkaloids isolated during the period of 2016–2021 and their relevant biological/pharmacological activities. The marine-derived indole alkaloids reported from 2016 to 2021 were collected from various scientific databases. A total of 186 indole alkaloids from various marine organisms including fungi, bacteria, sponges, bryozoans, mangroves, and algae, are described. Despite the described bioactivities, further evaluation including their mechanisms of action and biological targets is needed to determine which of these indole alkaloids are worth studying to obtain lead compounds for the development of new drugs.
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- 2021
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55. Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications.
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Ben Ali, Wissal, Chaduli, Delphine, Navarro, David, Lechat, Christian, Turbé-Doan, Annick, Bertrand, Emmanuel, Faulds, Craig B., Sciara, Giuliano, Lesage-Meessen, Laurence, Record, Eric, and Mechichi, Tahar
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LACCASE , *MICROBIAL enzymes , *ASPERGILLUS nidulans , *POLLUTION , *TRICHODERMA , *SUCROSE - Abstract
Background: Environmental pollution is one of the major problems that the world is facing today. Several approaches have been taken, from physical and chemical methods to biotechnological strategies (e.g. the use of oxidoreductases). Oxidative enzymes from microorganisms offer eco-friendly, cost–effective processes amenable to biotechnological applications, such as in industrial dye decolorization. The aim of this study was to screen marine-derived fungal strains isolated from three coastal areas in Tunisia to identify laccase-like activities, and to produce and characterize active cell-free supernatants of interest for dye decolorization. Results: Following the screening of 20 fungal strains isolated from the harbors of Sfax and Monastir (Tunisia), five strains were identified that displayed laccase-like activities. Molecular-based taxonomic approaches identified these strains as belonging to the species Trichoderma asperellum, Stemphylium lucomagnoense and Aspergillus nidulans. Among these five isolates, one T. asperellum strain (T. asperellum 1) gave the highest level of secreted oxidative activities, and so was chosen for further studies. Optimization of the growth medium for liquid cultures was first undertaken to improve the level of laccase-like activity in culture supernatants. Finally, the culture supernatant of T. asperellum 1 decolorized different synthetic dyes belonging to diverse dye families, in the presence or absence of 1-hydroxybenzotriazole (HBT) as a mediator. Conclusions: The optimal growth conditions to produce laccase-like active cell-free supernatants from T. asperellum 1 were 1.8 mM CuSO4 as an inducer, 1% NaCl to mimic a seawater environment and 3% sucrose as a carbon source. The culture supernatant of T. asperellum 1 effectively decolorized different synthetic dyes belonging to diverse chemical classes, and the presence of HBT as a mediator improved the decolorization process. [ABSTRACT FROM AUTHOR]
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- 2020
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56. New azaphilone, isocoumarin and α-pyrone derivatives from the marine-derived gut fungus Paraphaeosphaeria sp. XZD2-1.
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Sun, Jiaolu, Zhang, Haojian, Ding, Wanjing, Zheng, Dao-qiong, Wang, Pin-mei, and Xu, Jinzhong
- Abstract
Six new compounds including two azaphilones, lunatoic acids D–E (1 , 2), three isocoumarins, lunatinins B–D (3 - 5), and one α-pyrone derivative, lunatinin E (6), as well as four known ones, lunatoic acid A (7), lunatinin (8), penicipyran D (9) and chaetoquadrin F (10) were isolated from the rice medium of the gut fungus Paraphaeosphaeria sp. XZD2-1. Six new compounds including two azaphilones, lunatoic acids D–E (1 , 2), three isocoumarins, lunatinins B–D (3 - 5), and one α-pyrone derivative, lunatinin E (6), as well as four known ones, lunatoic acid A (7), lunatinin (8), penicipyran D (9) and chaetoquadrin F (10) were isolated from the rice medium of the gut fungus Paraphaeosphaeria sp. XZD2-1. Their planar structures were elucidated by analysis of 1D and 2D NMR as well as HRESIMS spectroscopic data. The absolute configurations of compounds 1 - 3 were assigned by experimental and calculated ECD data and an ester hydrolysis reaction. Compounds 4 and 5 were a pair of enantiomeric excess mixture. Structure of compound 6 was further confirmed by comparing optical rotation with known compounds according to literature. The antimicrobial activity and cytotoxicity of compounds 1 - 10 were examined. • The first report about lunatoic acids from a marine-derived Paraphaeosphaeria sp. fungus. • Four new isocoumarin and α-pyrone derivatives from the fungus. • Experimental and calculated ECD were used to elucidate the absolute configurations of new compounds. • The antimicrobial activity and cytotoxicity of isolated compounds were examined. [ABSTRACT FROM AUTHOR]
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- 2020
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57. De novo transcriptome assembly: a new laccase multigene family from the marine-derived basidiomycete Peniophora sp. CBMAI 1063
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Igor Vinicius Ramos Otero, Milene Ferro, Maurício Bacci, Henrique Ferreira, and Lara Durães Sette
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Marine-derived fungi ,Multicopper oxidase ,Laccase ,Transcriptome ,Biotechnology ,TP248.13-248.65 ,Microbiology ,QR1-502 - Abstract
Abstract Laccases are multicopper oxidases that are able to catalyze reactions involving a range of substrates, including phenols and amines, and this ability is related to the existence of different laccases. Basidiomycetes usually have more than one gene for laccase, but until now, this feature has not been demonstrated in a marine-derived fungus. Peniophora sp. CBMAI 1063 is a basidiomycete fungus isolated from a marine sponge that exhibits the ability to secrete significant amounts of laccase in saline conditions. In the present study, we identified laccase sequences from the transcriptome of Peniophora sp. CBMAI 1063 and used them to perform different molecular in silico analyses. The results revealed the presence of at least eight putative genes, which may encode ten different laccases with peptide lengths ranging from 482 to 588 aa and molecular weights ranging from 53.5 to 64.4 kDa. These laccases seem to perform extracellular activities, with the exception of one that may represent an intracellular laccase. The 10 predicted laccases expressed by Peniophora sp. CBMAI 1063 in laccase-induced media showed different patterns of N-glycosylation and isoelectric points and are divided into two classes based on the residue associated with the regulation of the redox potential of the enzyme. None of the predicted laccases showed more than 61% similarity to other fungal laccases. Based on the differences among the laccases expressed by Peniophora sp. CBMAI 1063, this marine-derived basidiomycete represents a valuable resource with strong potential for biotechnological exploitation.
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- 2017
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58. Asperfurandiones A and B, two antifungal furandione analogs from a marine-derived fungus Aspergillus versicolor.
- Author
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Ding, Lijian, Xu, Peng, Li, Te, Liao, Xiaojian, He, Shan, and Xu, Shihai
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ASPERGILLUS ,CRYPTOCOCCUS neoformans ,NUCLEAR magnetic resonance spectroscopy ,MASS spectrometry ,FUNGI - Abstract
Two new furandione derivatives, designated as asperfurandiones A (1) and B (2), were isolated from the solid culture of the marine-derived fungus Aspergillus versicolor. Their structures were elucidated using a combination of mass spectrometry and NMR spectroscopy. Additionally, asperfurandiones A and B both showed moderate antifungal activity against Gaeumannomyces graminis, Cryptococcus neoformans, and Candida albicans with MIC values of 64 μg/mL. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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59. Inhibitory effect of trichodermanone C, a sorbicillinoid produced by Trichoderma citrinoviride associated to the green alga Cladophora sp., on nitrite production in LPS-stimulated macrophages.
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Marra, Roberta, Nicoletti, Rosario, Pagano, Ester, DellaGreca, Marina, Salvatore, Maria Michela, Borrelli, Francesca, Lombardi, Nadia, Vinale, Francesco, Woo, Sheridan L., and Andolfi, Anna
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GREEN algae ,CLADOPHORA ,TRICHODERMA ,FUNGAL cultures ,METABOLITES ,METABOLIC profile tests - Abstract
From the green alga Cladophora sp. collected in Italy, the marine fungal strain A12 of Trichoderma citrinoviride was isolated, identified and characterized. LC-MS qTOF analysis was applied to perform a metabolic profile of the fungal culture. Chromatographic techniques and spectroscopic methods were used to isolate and characterize the major secondary metabolites produced by this strain in liquid culture. In particular, four known sorbicillinoids (trichodermanone C, spirosorbicillinol A, vertinolide and sorbicillin) were purified and identified, together with 2-phenylethanol and tyrosol. Moreover, metabolomic analysis allowed to detect small amounts of trichodimerol, rezishanone A, 2′,3′-dihydrosorbicillin and bisvertinol. For the first time a significant inhibitory effect on nitrite levels has been shown for trichodermanone C in lipopolysaccharide-stimulated J774A.1 macrophages. [ABSTRACT FROM AUTHOR]
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- 2019
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60. Structurally Diverse Polycyclic Salicylaldehyde Derivative Enantiomers from a Marine-Derived Fungus Eurotium sp. SCSIO F452
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Wei-Mao Zhong, Xiao-Yi Wei, Yu-Chan Chen, Qi Zeng, Jun-Feng Wang, Xue-Feng Shi, Xin-Peng Tian, Wei-Min Zhang, Fa-Zuo Wang, and Si Zhang
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marine-derived fungi ,Eurotium sp. ,natural products ,salicylaldehyde derivative enantiomers ,bioactivities ,Biology (General) ,QH301-705.5 - Abstract
To enlarge the chemical diversity of Eurotium sp. SCSIO F452, a talented marine-derived fungus, we further investigated its chemical constituents from a large-scale fermentation with modified culture. Four pairs of new salicylaldehyde derivative enantiomers, euroticins F-I (1–4), as well as a known one eurotirumin (5) were isolated and characterized. Compound 1 features an unprecedented constructed 6/6/6/5 tetracyclic structures, while 2 and 3 represent two new types of 6/6/5 scaffolds. Their structures were established by comprehensive spectroscopic analyses, X-ray diffraction, 13C NMR, and electronic circular dichroism calculations. Selected compounds showed significant inhibitory activity against α-glucosidase and moderate cytotoxic activities against SF-268, MCF-7, HepG2, and A549 cell lines.
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- 2021
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61. Marine-Derived Fungi as a Valuable Resource for Amylases Activity Screening
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Chen, Di Zhang, Lan Liu, and Bi-Shuang
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marine-derived fungi ,amylase ,starch hydrolysis ,enzyme activity ,thermal and pH stability - Abstract
Marine microbial enzymes including amylases are important in different industrial production due to their properties and applications. This study was focused on the screening of marine-derived fungi for amylase activities. First, we isolated a number of fungi from the sediments of the South China Sea. By the method of dish screening (in vitro), we subsequently obtained a series of amylase-producing fungal strains. The cell-lysate activities of amylases produced by marine fungi toward starch hydrolysis were achieved with the dinitrosalyicylic acid (DNS) method. In addition, the effect of pH and temperature on amylase activities, including thermal and pH stability were discussed. Results showed that out of the 57 isolates with amylase-producing activities, fungi Aspergillus flavus 9261 was found to produce amylase with the best activity of 10.7482 U/mg (wet mycelia). The amylase of Aspergillus flavus 9261 exhibited remarkable thermostability and pH stability with no activity loss after incubation at 50 °C and pH 5.0 for 1 h, respectively. The results provide advances in discovering enzymes from marine-derived fungi and their biotechnology relevance.
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- 2023
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62. Meroterpenoids from Marine Microorganisms: Potential Scaffolds for New Chemotherapy Leads
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Gomes, Nelson G. M., Buttachon, Suradet, Kijjoa, Anake, and Kim, Se-Kwon, editor
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- 2015
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63. Isolation and Characterization of Nickel-Tolerant Trichoderma Strains from Marine and Terrestrial Environments
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Jewel C. De Padua and Thomas Edison E. dela Cruz
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agar culture ,bioremediation ,heavy metal tolerance ,marine-derived fungi ,pollution ,Biology (General) ,QH301-705.5 - Abstract
Nickel contamination is a serious environmental issue that requires immediate action. In this study, 23 strains of Trichoderma were isolated from terrestrial and marine environments and identified using a polyphasic approach of morphological characterization and ITS gene sequence analysis. The Trichoderma strains were tested for their tolerance and biosorption of nickel. Our results showed the growth of all Trichoderma strains on Trichoderma Selective Medium (TSM) with 50–1200-ppm nickel, indicating their tolerance of this heavy metal even at a relatively high concentration. Six Trichoderma strains (three isolated from terrestrial substrates and three from marine substates) had the highest radial growth on TSM with 50-ppm Ni. Among these fungal isolates, Trichoderma asperellum (S03) isolated from soil exhibited the best growth after 2 days of incubation. For the biosorption of nickel, the accumulation or uptake efficiency by the six selected Trichoderma was determined in Potato Dextrose Broth (PDB) supplemented with 50-ppm Ni using a Flame Atomic Absorption Spectrophotometer (AAS). The percent uptake efficiency of the three strains of T. asperellum (S03, S08, and LL14) was computed to be up to 66%, while Trichoderma virens (SG18 and SF22) and Trichoderma inhamatum (MW25) achieved up to 68% uptake efficiency. Observation of the Trichoderma strains with Scanning Electron Microscopy (SEM) before and after the absorption of nickel showed very minimal damage on the hyphal and conidial surface morphology, but changes in the colonial characteristics were observed. Our study highlighted the potential of terrestrial and marine strains of Trichoderma for the bioremediation of nickel pollution.
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- 2021
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64. Ochraceopetalin, a Mixed-Biogenetic Salt of Polyketide and Amino Acid Origins from a Marine-Derived Aspergillus ochraceopetaliformis Fungus
- Author
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Sung Chul Park, Jung-Ho Lee, Ji-Yeon Hwang, Oh-Seok Kwon, Lijuan Liao, Dong-Chan Oh, Ki-Bong Oh, and Jongheon Shin
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organic salt ,diphenylether ,Aspergillus ochraceopetaliformis ,marine-derived fungi ,Biology (General) ,QH301-705.5 - Abstract
Ochraceopetalin (1), a mixed-biogenetic salt compound and its component 2 were isolated from the culture broths of a marine-derived fungus, Aspergillus ochraceopetaliformis. Based on combined spectroscopic and chemical analyses, the structure of 1 was determined to be a sulfonated diphenylether-aminol-amino acid ester guanidinium salt of an unprecedented structural class, while 2 was determined to be the corresponding sulfonated diphenylether. Ochraceopetaguanidine (3), the other guanidine-bearing aminol amino acid ester component, was also prepared and structurally elucidated. Compound 1 exhibited significant cytotoxicity against K562 and A549 cells.
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- 2021
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65. Polyketides and Meroterpenes from the Marine-Derived Fungi Aspergillus unguis 158SC-067 and A. flocculosus 01NT-1.1.5 and Their Cytotoxic and Antioxidant Activities
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Cao Van Anh, Jong Soon Kang, Byeoung-Kyu Choi, Hwa-Sun Lee, Chang-Su Heo, and Hee Jae Shin
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marine-derived fungi ,Aspergillus sp. ,polyketides ,meroterpenes ,antioxidant ,cytotoxicity ,Biology (General) ,QH301-705.5 - Abstract
Ten secondary metabolites, including a new grifolin analog, grifolin B (1); a new homovalencic acid derivative, 12-hydroxyhomovalencic acid (7); and a compound isolated from a natural source for the first time (9), along with seven known compounds, grifolin (2), averantin (3), 7-chloroaverantin (4), 1′-O-methylaverantin (5), 7-hydroxy-2-(2-hydroxypropyl)-5-pentylchromone (6), homovalencic acid (8), and bekeleylactone E (10), were isolated from two fungal strains. The structures of 1–10 were identified by detailed analysis and comparison of their spectroscopic data with literature values. Compounds 9 and 10 showed moderate cytotoxic activity against a panel of cancer cell lines (PC-3, HCT-15, MDA-MB-231, ACHN, NCI-H23, NUGC-3), with the GI50 values ranging from 1.1 µM to 3.6 µM, whereas 1 displayed a weak 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity without cytotoxicity against all tested cell lines.
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- 2021
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66. ISOLATION, IDENTIFICATION AND BIOACTIVITY SCREENING OF TURKISH MARINE-DERIVED FUNGI.
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HEYDARI, HAJAR, KOC, ASLI, SIMSEK, DUYGU, GOZCELIOGLU, BULENT, ALTANLAR, NURTEN, and KONUKLUGIL, BELMA
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PENICILLIUM ,ASPERGILLUS niger ,FUNGI ,MARINE invertebrates ,ALTERNARIA ,METABOLITES - Abstract
Copyright of Farmacia is the property of Societatea de Stiinte Farmaceutice Romania and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2019
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67. Antioxidant and cytotoxic activity of three Turkish marine-derived fungi.
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Gözcelioğlu, Bülent
- Subjects
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METABOLITES , *FUNGI , *ASPERGILLUS flavus , *MARINE invertebrates , *COLON cancer , *ASPERGILLUS , *MALASSEZIA - Abstract
Background: Marine-derived fungi are appraised as a favorable source for discovering new bioactive secondary metabolites. In the last few decades researchers have concentrated on marine-derived fungi to obtain new and pharmaceutically active bioactive secondary metabolites with therapeutic potential. Objective: In this study three marine-derived fungi were isolated and identified from marine invertebrates and investigated with regard to their antioxidant and cytotoxic activities. Materials and methods: DPPH, SO, NO, and ABTS assays were used for monitoring free radical scavenging activity, and the MTT assay was used for testing cytotoxic activity against HCT-116 colon cancer cells. Results: According to the obtained results Malassezia restricta extract was shown to have the highest antioxidant and cytotoxic activities compared to the other tested fungi strains. Conclusion: This study is the first report about the antioxidant and cytotoxicity activity of Acremonium sclerotigenum, Aspergillus flavus, and M. restricta. This serves as a valuable preliminary study for activity-guided isolation of secondary metabolites. [ABSTRACT FROM AUTHOR]
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- 2019
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68. (+)- and (−)-Eurotone A: A pair of enantiomeric polyketide dimers from a marine-derived fungus Eurotium sp. SCSIO F452.
- Author
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Zhong, Wei-Mao, Wang, Jun-Feng, Wei, Xiao-Yi, Zeng, Qi, Chen, Xia-Yu, Xiang, Yao, Tian, Xin-Peng, Zhang, Si, Long, Li-Juan, and Wang, Fa-Zuo
- Subjects
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POLYKETIDES , *DIMERS , *MARINE fungi , *FUNGI , *ENANTIOMERS , *DIMERIZATION - Abstract
• (±)-Eurotone A was identified from the marine fungus Eurotium sp. SCSIO F452. • (±)-Eurotone A was a pair of spirodihydrobenzanthracene enantiomers. • The absolute configuration of (±)-eurotone A was evidenced by ECD calculations. (±)-Eurotone A [(±)- 1 ], a pair of new enantiomeric polyketide dimers, as well as six known biogenetically related polyketides (2 – 7) were isolated from a marine-derived fungus Eurotium sp. SCSIO F452. Their structures were determined by comprehensive spectroscopic methods, X-ray diffraction and quantum chemical calculations. Compound 1 represented the first pair of spirodihydrobenzanthracene enantiomers isolated from marine fungi with their absolute configurations assigned. A plausible biosynthetic pathway involving a key acid-mediated dimerization was proposed for 1. The antioxidative activities of the new enantiomers were evaluated. [ABSTRACT FROM AUTHOR]
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- 2019
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69. Discovery of a new biphenyl derivative by epigenetic manipulation of marine-derived fungus Aspergillus versicolor.
- Author
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Zhu, Jing Xuan, Ding, Lijian, and He, Shan
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BIPHENYL compounds ,ASPERGILLUS ,HISTONE deacetylase inhibitors ,HYDROXAMIC acids ,METABOLITES ,STAPHYLOCOCCUS aureus - Abstract
Cultivation of the marine-derived Aspergillus versicolor MCCC 3A00080 with the addition of a histone deacetylase inhibitor, suberoylanilide hydroxamic acid (SAHA), significantly enhanced the diversity of secondary metabolites. From the culture treated, a new biphenyl derivative, named versiperol A (1), along with two known compounds, 2,4-dimethoxyphenol (2) and diorcinol (3) were isolated. The structures of 1–3 were established based on spectroscopic analysis and comparison with literature data. Among the isolates, versiperol A (1) exhibited modest inhibition of Staphylococcus aureus growth with MIC value of 8 μg/mL. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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70. Butenolides from a marine-derived fungus Aspergillus terreus with antitumor activities against pancreatic ductal adenocarcinoma cells.
- Author
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Qi, Changxing, Gao, Weixi, Guan, Danyingzi, Wang, Jianping, Liu, Mengting, Chen, Chunmei, Zhu, Hucheng, Zhou, Yuan, Lai, Yongji, Hu, Zhengxi, Zhou, Qun, and Zhang, Yonghui
- Subjects
- *
BUTENOLIDES , *ASPERGILLUS terreus , *ANTINEOPLASTIC agents , *PANCREATIC cancer , *APOPTOSIS - Abstract
Graphical abstract Abstract Chemical study on the extract of a marine-derived fungus Aspergillus terreus yielded twelve butenolide derivatives, including three new compounds, namely asperlides A–C (1 – 3) and nine known butenolides (4 – 12). The structures of 1 – 3 were confirmed by comprehensive spectroscopic analysis, including HRESIMS, NMR spectroscopy, and calculated electronic circular dichroism (ECD). The cytotoxicity of the compounds was evaluated using PANC-1, HCC1806, HepG2, BEAS-2B and HT-29 cancer cells. The results showed that (+)-3′,3′-di-(dimethylallyl)-butyrolactone II (4) and versicolactone B (6) exhibited the most potent cytotoxin of PANC-1 cell line, with the IC 50 values of 5.3 and 9.4 μM, respectively. Morphological features of apoptosis were observed in 4 and 6 -treated PANC-1 cells, including apoptotic body formation, membrane blebbing, cell shrinkage and nuclear condensation. Cell cycle analysis with propidium iodide staining exhibited that 4 inhibits proliferation of PANC-1 cells via the induction of G 2 /M and S phase arrest, while 6 could retard the PANC-1 cells via the induction of S phase arrest. Flow cytometric analysis suggested that treatment with 4 and 6 significantly induced PANC-1 cells apoptosis. These findings indicated that 4 and 6 might serve as a starting point for the development of an anticancer drug for the treatment of pancreatic ductal adenocarcinoma. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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71. Neuroprotective Metabolites from Vietnamese Marine Derived Fungi of Aspergillus and Penicillium Genera
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Elena V. Girich, Anton N. Yurchenko, Olga F. Smetanina, Phan Thi Hoai Trinh, Ngo Thi Duy Ngoc, Mikhail V. Pivkin, Roman S. Popov, Evgeny A. Pislyagin, Ekaterina S. Menchinskaya, Ekaterina A. Chingizova, Shamil S. Afiyatullov, and Ekaterina A. Yurchenko
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Aspergillus terreus ,Aspergillus flocculosus ,Penicillium sp. ,marine-derived fungi ,South China Sea ,secondary metabolites ,Biology (General) ,QH301-705.5 - Abstract
Low molecular weight secondary metabolites of marine fungi Aspergillus flocculosus, Aspergillus terreus and Penicillium sp. from Van Phong and Nha Trang Bays (Vietnam) were studied and a number of polyketides, bis-indole quinones and terpenoids were isolated. The structures of the isolated compounds were determined by 1D and 2D NMR and HR-ESI-MS techniques. Stereochemistry of some compounds was established based on ECD data. A chemical structure of asterriquinone F (6) was thoroughly described for the first time. Anthraquinone (13) was firstly obtained from a natural source. Neuroprotective influences of the isolated compounds against 6-OHDA, paraquat and rotenone toxicity were investigated. 4-Hydroxyscytalone (1), 4-hydroxy-6-dehydroxyscytalone (2) and demethylcitreoviranol (3) have shown significant increasing of paraquat- and rotenone-treated Neuro-2a cell viability and anti-ROS activity.
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- 2020
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72. Bioassay-guided fractionation and identification of active substances from the fungus Aspergillus tubingensis against Vibrio anguillarum
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Lei Guo, Cong Wang, Wen-cheng Zhu, and Fu-quan Xu
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marine-derived fungi ,aspergillus tubingensis ,antibacterial activity ,vibrio anguillarum ,fractionation and identification ,Biotechnology ,TP248.13-248.65 - Abstract
The aim of this study was to perform bioassay-guided fractionation and identification of active substances against the aquatic pathogen Vibrio anguillarum from the fungus Aspergillus tubingensis SZX-6 associated with the crab Portunus trituberculatus. Based on the antibacterial activity against V. anguillarum, three compounds were isolated and purified from the culture broth of strain SZX-6 by sequential column chromatography over silica gel, Sephadex LH-20 and RP-18. The isolated compounds were identified on the basis of extensive spectroscopic data analysis. They were methyl 6-acetyl-5,7,8-trihydroxy-4-methoxy-2-naphthoate (1), daidzein (2) and genistein (3). Compound 1 showed moderate antibacterial activity against V. anguillarum (minimum inhibitory concentration 0.063 mg/mL). The obtained results could serve as a basis for further research towards design of active antibacterial agents against aquatic pathogens.
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- 2016
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73. Chemical Diversity and Biological Activities of Meroterpenoids from Marine Derived-Fungi: A Comprehensive Update
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Amr El-Demerdash, Decha Kumla, and Anake Kijjoa
- Subjects
meroterpenoids ,marine-derived fungi ,biological activities ,antibacterial ,cytotoxicity ,anti-inflammatory ,Biology (General) ,QH301-705.5 - Abstract
Meroterpenoids are a class of hybrid natural products, partially derived from a mixed terpenoid pathway. They possess remarkable structural features and relevant biological and pharmacological activities. Marine-derived fungi are a rich source of meroterpenoids featuring structural diversity varying from simple to complex molecular architectures. A combination of a structural variability and their myriad of bioactivities makes meroterpenoids an interesting class of naturally occurring compounds for chemical and pharmacological investigation. In this review, a comprehensive literature survey covering the period of 2009–2019, with 86 references, is presented focusing on chemistry and biological activities of various classes of meroterpenoids isolated from fungi obtained from different marine hosts and environments.
- Published
- 2020
- Full Text
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74. Degradation Mechanism of 2,4-Dichlorophenol by Fungi Isolated from Marine Invertebrates
- Author
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Efstratios Nikolaivits, Andreas Agrafiotis, Eirini Baira, Géraldine Le Goff, Nikolaos Tsafantakis, Suchana A. Chavanich, Yehuda Benayahu, Jamal Ouazzani, Nikolas Fokialakis, and Evangelos Topakas
- Subjects
2,4-dichlorophenol ,marine-derived fungi ,invertebrate symbionts ,catechol dioxygenase ,DCP metabolites ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
2,4-Dichlorophenol (2,4-DCP) is a ubiquitous environmental pollutant categorized as a priority pollutant by the United States (US) Environmental Protection Agency, posing adverse health effects on humans and wildlife. Bioremediation is proposed as an eco-friendly, cost-effective alternative to traditional physicochemical remediation techniques. In the present study, fungal strains were isolated from marine invertebrates and tested for their ability to biotransform 2,4-DCP at a concentration of 1 mM. The most competent strains were studied further for the expression of catechol dioxygenase activities and the produced metabolites. One strain, identified as Tritirachium sp., expressed high levels of extracellular catechol 1,2-dioxygenase activity. The same strain also produced a dechlorinated cleavage product of the starting compound, indicating the assimilation of the xenobiotic by the fungus. This work also enriches the knowledge about the mechanisms employed by marine-derived fungi in order to defend themselves against chlorinated xenobiotics.
- Published
- 2020
- Full Text
- View/download PDF
75. Anti-Inflammatory and Protein Tyrosine Phosphatase 1B Inhibitory Metabolites from the Antarctic Marine-Derived Fungal Strain Penicillium glabrum SF-7123
- Author
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Tran Minh Ha, Dong-Cheol Kim, Jae Hak Sohn, Joung Han Yim, and Hyuncheol Oh
- Subjects
marine-derived fungi ,anti-inflammation ,anti-neuroinflammation ,PTP1B ,Biology (General) ,QH301-705.5 - Abstract
A chemical investigation of the marine-derived fungal strain Penicillium glabrum (SF-7123) revealed a new citromycetin (polyketide) derivative (1) and four known secondary fungal metabolites, i.e, neuchromenin (2), asterric acid (3), myxotrichin C (4), and deoxyfunicone (5). The structures of these metabolites were identified primarily by extensive analysis of their spectroscopic data, including NMR and MS data. Results from the initial screening of anti-inflammatory effects showed that 2, 4, and 5 possessed inhibitory activity against the excessive production of nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated BV2 microglial cells, with IC50 values of 2.7 µM, 28.1 µM, and 10.6 µM, respectively. Compounds 2, 4, and 5 also inhibited the excessive production of NO, with IC50 values of 4.7 µM, 41.5 µM, and 40.1 µM, respectively, in LPS-stimulated RAW264.7 macrophage cells. In addition, these compounds inhibited LPS-induced overproduction of prostaglandin E2 in both cellular models. Further investigation of the most active compound (2) revealed that these anti-inflammatory effects were associated with a suppressive effect on the over-expression of inducible nitric oxide synthase and cyclooxygenase-2. Finally, we showed that the anti-inflammatory effects of compound 2 were mediated via the downregulation of inflammation-related pathways such as those dependent on nuclear factor kappa B and p38 mitogen-activated protein kinase in LPS-stimulated BV2 and RAW264.7 cells. In the evaluation of the inhibitory effects of the isolated compounds on protein tyrosine phosphate 1B (PTP1B) activity, compound 4 was identified as a noncompetitive inhibitor of PTP1B, with an IC50 value of 19.2 µM, and compound 5 was shown to inhibit the activity of PTP1B, with an IC50 value of 24.3 µM, by binding to the active site of the enzyme. Taken together, this study demonstrates the potential value of marine-derived fungal isolates as a bioresource for bioactive compounds.
- Published
- 2020
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76. Secondary Metabolites from the Culture of the Marine-derived Fungus Paradendryphiella salina PC 362H and Evaluation of the Anticancer Activity of Its Metabolite Hyalodendrin
- Author
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Ambre Dezaire, Christophe H. Marchand, Marine Vallet, Nathalie Ferrand, Soraya Chaouch, Elisabeth Mouray, Annette K. Larsen, Michèle Sabbah, Stéphane D. Lemaire, Soizic Prado, and Alexandre E. Escargueil
- Subjects
anticancer agent ,marine-derived fungi ,secondary metabolites ,resistant phenotypes ,epidithiodioxopiperazines ,Biology (General) ,QH301-705.5 - Abstract
High-throughput screening assays have been designed to identify compounds capable of inhibiting phenotypes involved in cancer aggressiveness. However, most studies used commercially available chemical libraries. This prompted us to explore natural products isolated from marine-derived fungi as a new source of molecules. In this study, we established a chemical library from 99 strains corresponding to 45 molecular operational taxonomic units and evaluated their anticancer activity against the MCF7 epithelial cancer cell line and its invasive stem cell-like MCF7-Sh-WISP2 counterpart. We identified the marine fungal Paradendryphiella salina PC 362H strain, isolated from the brown alga Pelvetia caniculata (PC), as one of the most promising fungi which produce active compounds. Further chemical and biological characterizations of the culture of the Paradendryphiella salina PC 362H strain identified (-)-hyalodendrin as the active secondary metabolite responsible for the cytotoxic activity of the crude extract. The antitumor activity of (-)-hyalodendrin was not only limited to the MCF7 cell lines, but also prominent on cancer cells with invasive phenotypes including colorectal cancer cells resistant to chemotherapy. Further investigations showed that treatment of MCF7-Sh-WISP2 cells with (-)-hyalodendrin induced changes in the phosphorylation status of p53 and altered expression of HSP60, HSP70 and PRAS40 proteins. Altogether, our study reveals that this uninvestigated marine fungal crude extract possesses a strong therapeutic potential against tumor cells with aggressive phenotypes and confirms that members of the epidithiodioxopiperazines are interesting fungal toxins with anticancer activities.
- Published
- 2020
- Full Text
- View/download PDF
77. A New Citrinin Derivative from the Indonesian Marine Sponge-Associated Fungus Penicillium citrinum
- Author
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Aninditia Sabdaningsih, Yang Liu, Ute Mettal, John Heep, Riyanti, Lei Wang, Olvi Cristianawati, Handung Nuryadi, Mada Triandala Sibero, Michael Marner, Ocky Karna Radjasa, Agus Sabdono, Agus Trianto, and Till F. Schäberle
- Subjects
antibacterial ,citrinin derivatives ,marine-derived fungi ,Penicillium citrinum ,penicitrinone ,marine sponges ,Biology (General) ,QH301-705.5 - Abstract
Sponge-associated fungi are attractive targets for the isolation of bioactive natural products with different pharmaceutical purposes. In this investigation, 20 fungi were isolated from 10 different sponge specimens. One isolate, the fungus Penicillium citrinum strain WK-P9, showed activity against Bacillus subtilis JH642 when cultivated in malt extract medium. One new and three known citrinin derivatives were isolated from the extract of this fungus. The structures were elucidated by 1D and 2D NMR spectroscopy, as well as LC-HRMS. Their antibacterial activity against a set of common human pathogenic bacteria and fungi was tested. Compound 2 showed moderate activity against Mycobacterium smegmatis ATCC607 with a minimum inhibitory concentration (MIC) of 32 µg/mL. Compound 4 exhibited moderate growth inhibition against Bacillus subtilis JH642, B. megaterium DSM32, and M. smegmatis ATCC607 with MICs of 16, 16, and 32 µg/mL, respectively. Furthermore, weak activities of 64 µg/mL against B. subtilis DSM10 and S. aureus ATCC25923 were observed for compound 4.
- Published
- 2020
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78. Identification of the Main Metabolites of a Marine-Derived Strain of Penicillium brevicompactum Using LC and GC MS Techniques
- Author
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Francesco Vinale, Maria Michela Salvatore, Rosario Nicoletti, Alessia Staropoli, Gelsomina Manganiello, Tommaso Venneri, Francesca Borrelli, Marina DellaGreca, Francesco Salvatore, and Anna Andolfi
- Subjects
epidithiodioxopiperazines ,marine-derived fungi ,antiproliferative activity ,anemonia sulcata ,mycophenolic acid ,thiosilvatins ,beneficial microbes ,Microbiology ,QR1-502 - Abstract
Marine-derived fungi are an important source of many valuable compounds with original structures and diverse physico-chemical properties. In this work, the metabolomic profile of a strain of Penicillium brevicompactum, recovered from a snakelocks sea anemone (Anemonia sulcata), was investigated through the parallel application of LC-ESI-HRMS, GC-MS, and NMR. Our strategy allowed the identification of mycophenolic acid, brevianamide A, and several compounds belonging to the thiosilvatins. Among the latter, five products are reported for the first time in this species. The main product of this series, cis-bis(methylthio)silvatin, was also tested for antiproliferative activity on both cancer and non-tumoral colon cell lines.
- Published
- 2020
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79. A Review of Anti-Inflammatory Compounds from Marine Fungi, 2000–2018
- Author
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Jianzhou Xu, Mengqi Yi, Lijian Ding, and Shan He
- Subjects
marine-derived fungi ,marine natural products ,anti-inflammatory ,Biology (General) ,QH301-705.5 - Abstract
Inflammation is a generalized, nonspecific, and beneficial host response of foreign challenge or tissue injury. However, prolonged inflammation is undesirable. It will cause loss function of involve organs, such as heat, pain redness, and swelling. Marine natural products have gained more and more attention due to their unique mechanism of anti-inflammatory action, and have considered a hotspot for anti-inflammatory drug development. Marine-derived fungi are promising sources of structurally unprecedented bioactive natural products. So far, a plethora of new secondary metabolites with anti-inflammatory activities from marine-derived fungi had been widely reported. This review covers 133 fungal metabolites described in the period of 2000 to 2018, including the structures and origins of these secondary metabolites.
- Published
- 2019
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- View/download PDF
80. Biotechnology Potential of Marine Fungi Degrading Plant and Algae Polymeric Substrates
- Author
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Larissa Balabanova, Lubov Slepchenko, Oksana Son, and Liudmila Tekutyeva
- Subjects
filamentous fungi ,marine-derived fungi ,glycoside hydrolases ,algae polysaccharides ,plant polysaccharide-degrading enzymes ,lignocellulolytic enzymes ,Microbiology ,QR1-502 - Abstract
Filamentous fungi possess the metabolic capacity to degrade environment organic matter, much of which is the plant and algae material enriched with the cell wall carbohydrates and polyphenol complexes that frequently can be assimilated by only marine fungi. As the most renewable energy feedstock on the Earth, the plant or algae polymeric substrates induce an expression of microbial extracellular enzymes that catalyze their cleaving up to the component sugars. However, the question of what the marine fungi contributes to the plant and algae material biotransformation processes has yet to be highlighted sufficiently. In this review, we summarized the potential of marine fungi alternatively to terrestrial fungi to produce the biotechnologically valuable extracellular enzymes in response to the plant and macroalgae polymeric substrates as sources of carbon for their bioconversion used for industries and bioremediation.
- Published
- 2018
- Full Text
- View/download PDF
81. Isolation and Synthesis of Misszrtine A: A Novel Indole Alkaloid From Marine Sponge-Associated Aspergillus sp. SCSIO XWS03F03
- Author
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Rong Zhou, Xiaojian Liao, Hangbin Li, Jing Li, Pengju Feng, BingXin Zhao, and Shihai Xu
- Subjects
indole alkaloid ,marine-derived fungi ,secondary metabolites ,sponge ,total synthesis ,antagonistic activity ,Chemistry ,QD1-999 - Abstract
A novel indole alkaloid, misszrtine A (1), was isolated from marine sponge-derived fungus Aspergillus sp. SCSIO XWS03F03. The planar structure of 1 was assigned by analysis of spectroscopic data, the absolute configuration of which was unambiguously determined by total synthesis. Compound 1 represents the first example of N-isopentenyl tryptophan methyl ester with a phenylpropanoic amide arm, which exhibited a potent antagonistic activity on HL60 (IC50 = 3.1 μM) and LNCaP (IC50 = 4.9 μM) cell lines. Bioactivity evaluation reveals that functional group on indole nitrogen of 1 has a great effect on its cytotoxity, which provides a mean to probe the structure-activity relationships of 1.
- Published
- 2018
- Full Text
- View/download PDF
82. Bioactive Terpenes from Marine-Derived Fungi
- Author
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Ahmed M. Elissawy, Mohamed El-Shazly, Sherif S. Ebada, AbdelNasser B. Singab, and Peter Proksch
- Subjects
marine-derived fungi ,secondary metabolites ,terpenes ,bioactivity ,Biology (General) ,QH301-705.5 - Abstract
Marine-derived fungi continue to be a prolific source of secondary metabolites showing diverse bioactivities. Terpenoids from marine-derived fungi exhibit wide structural diversity including numerous compounds with pronounced biological activities. In this review, we survey the last five years’ reports on terpenoidal metabolites from marine-derived fungi with particular attention on those showing marked biological activities.
- Published
- 2015
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83. Aspertides A-E: Antimicrobial Pentadepsipeptides with a Unique p -Methoxycinnamoyl Amide Group from the Marine Isolates Aspergillus tamarii MA-21 and Aspergillus insuetus SD-512.
- Author
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Chi LP, Liu D, Li XM, Wan Y, Wang BG, and Li X
- Subjects
- Amides, Aspergillus, Anti-Infective Agents pharmacology
- Abstract
Marine fungus-derived natural products are an important source of antimicrobial compounds against marine aquatic pathogens. Here, we describe the isolation and characterization of five new pentadepsipeptides, aspertides A-E ( 1 - 5 ), containing a unique p -methoxycinnamoyl amide group, from the marine fungi Aspergillus tamarii MA-21 and Aspergillus insuetus SD-512. Among them, aspertides B-E ( 2 - 5 ) also possessed uncommon amino acid residues, such as 3-hydroxyproline, 2,3-dihydroxyproline, or pipecolinic acid. The structures of these compounds were elucidated on the basis of NMR and mass spectroscopic analyses. The absolute configurations of them were established by chiral HPLC analyses of the acidic hydrolysates and NMR calculations with DP4+ probability analysis. In bio-activity assays, compounds 4 and 5 exhibited antibacterial activities against aquatic-pathogenic bacteria, including Edwardsiella tarda , Vibrio alginolyticus , Vibrio anguillarum , Vibrio vulnificus, and Staphylococcus aureus, with MIC values of 8-32 μg/mL.
- Published
- 2023
- Full Text
- View/download PDF
84. GC–MS approaches for the screening of metabolites produced by marine-derived Aspergillus.
- Author
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Salvatore, Maria Michela, Nicoletti, Rosario, Salvatore, Francesco, Naviglio, Daniele, and Andolfi, Anna
- Subjects
- *
METABOLITES , *ASPERGILLUS , *METABOLOMICS , *MASS spectrometry , *GAS chromatography , *ASCOMYCETES - Abstract
Abstract In the last decades the increasing awareness by the scientific community of the importance to exploit natural resources for the finding of new bioactive products has stimulated a huge research activity concerning the marine biological resources, and advanced strategies have been developed for investigations on microbial metabolomics. Gas chromatography coupled to mass spectrometry represents a valuable technology in this respect. The present review considers general GC–MS approaches to be adopted in investigations on fungal metabolomics, and more particularly the outcomes concerning marine-derived strains of Aspergillus , representing the most widespread genus of Ascomycetes fungi reported from the sea. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
85. Synthesis of α-chloroacetophenones with NH4Cl/Oxone® in situ followed by bioreduction with whole cells of marine-derived fungi.
- Author
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Morais, Aline T.do B., Ferreira, Irlon M., Jimenez, David E.Q., and Porto, André L.M.
- Subjects
ACETOPHENONE synthesis ,AMMONIUM chloride ,CHLOROHYDRINS ,INTERMEDIATES (Chemistry) ,BIOACTIVE compounds ,KETONES - Abstract
Abstract Chiral chlorohydrins are used as intermediates in the synthesis of various compounds with biological activities. This paper reports the synthesis of α-chloroketones 2a–c with oxone
® and NH 4 Cl at reflux via 30 min of exposure to microwave irradiation and conventional heating, in situ, followed by reduction with whole cells of marine-derived fungi (Penicillium citrinum CBMAI 1186, Mucor racemosus CBMAI 847, Aspergillus sydowii CBMAI 935, Penicillium raistrickii CBMAI 931, and Penicillium oxalicum CBMAI 1185), yielding the respective chlorohydrins 3a–c with good conversion (32–97%) and enantioselectivities (60–94%). This is the first study involving the synthesis of α-chlorophenones in situ followed by biocatalytic reduction from whole cells of marine-derived fungi to obtain enantio-enriched chlorohydrins 3a–c. Graphical abstract fx1 Highlights • Synthesis of α-chloroketones with oxone® and NH 4 Cl under microwave irradiation and conventional heating. • Synthesis of α-chlorophenones in situ followed by biocatalytic reduction. • Fungi marine used in the obtaining of chiral chlorohydrins. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
86. Prenylated Diphenyl Ethers from the Marine Algal-Derived Endophytic Fungus Aspergillus tennesseensis.
- Author
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Zhao-Xia Li, Xiu-Fang Wang, Guang-Wei Ren, Xiao-Long Yuan, Ning Deng, Gui-Xia Ji, Wei Li, and Peng Zhang
- Subjects
- *
METABOLITES , *PHENYL ethers , *ASPERGILLUS terreus , *NUCLEAR magnetic resonance , *ENDOPHYTIC bacteria - Abstract
Considerable attention has been paid to marine derived endophytic fungi, owing to their capacity to produce novel secondary metabolites with potent bioactivities. In this study, two new compounds with a prenylated diphenyl ether structure--diorcinol L (1) and (R)-diorcinol B (2)--were isolated from the marine algal-derived endophytic fungus Aspergillus tennesseensis, along with seven known compounds: (S)-diorcinol B (3), 9-acetyldiorcinol B (4), diorcinol C (5), diorcinol D (6), diorcinol E (7), diorcinol J (8), and a dihydrobenzofuran derivative 9. Their structures were elucidated by extensive NMR spectroscopy studies. Compound 2 represents the first example of an R-configuration in the prenylated moiety. All these isolated compounds were examined for antimicrobial and cytotoxic activities. Compounds 1-9 exhibited antimicrobial activities against some human- and plant-pathogenic microbes with MIC values ranging from 2 to 64 µg/mL. Moreover, compound 9 displayed considerable inhibitory activity against the THP-1 cell line in vitro, with an IC50 value of 7.0 µg/mL. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
87. New bioactive chlorinated cyclopentene derivatives from the marine-derived Fungus Phoma sp.
- Author
-
Elsebai, Mahmoud Fahmi, Ghabbour, Hazem A., Legrave, Nathalie, Fontaine-Vive, Fabien, and Mehiri, Mohamed
- Abstract
The new halogenated metabolites cryptophomic acid (1), cryptodiol (2), and cryptotriol (3) and the known dihydro-isocoumarin derivative 4, were isolated from the marine-derived fungus Phoma sp.135. The structural elucidation of these compounds was achieved by extensive analysis of spectroscopic data including 1D- and 2D-NMR, HRMS, optical rotation, UV, and IR. The absolute configuration of cryptophomic acid (1) was determined by using circular dichroism and TD-DFT ECD calculations of the solution conformers. The relative configurations of cryptodiol (2) and cryptotriol (3) were elucidated by a detailed analysis of the spectroscopic data and quantum mechanical calculation of NMR chemical shifts, based also on the newly reported + approach. The dihydro-isocoumarin derivative 4 was also produced by the same fungus and its structure was established as (R)-8-hydroxy-6-methoxy-3-methy-3,4-dihydro-isocoumarin (4), often referred to 6-methoxymellein and its chemical structure and absolute configuration were further confirmed through X-ray diffraction analysis. We report here the new compounds 1-3 and the crystallographic data of compound 4 for the first time. Additionally, we report significant antimicrobial activity of compounds 1-3 against Escherichia coli, Bacillus subtilis, Mycobacterium phlei, and Staphylococcus aureus and they showed no lethality against brine shrimp.
[ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
88. Biotechnology Potential of Marine Fungi Degrading Plant and Algae Polymeric Substrates.
- Author
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Balabanova, Larissa, Slepchenko, Lubov, Son, Oksana, and Tekutyeva, Liudmila
- Subjects
MARINE biology ,PLANT biotechnology ,BIODEGRADATION - Abstract
Filamentous fungi possess the metabolic capacity to degrade environment organic matter, much of which is the plant and algae material enriched with the cell wall carbohydrates and polyphenol complexes that frequently can be assimilated by only marine fungi. As the most renewable energy feedstock on the Earth, the plant or algae polymeric substrates induce an expression of microbial extracellular enzymes that catalyze their cleaving up to the component sugars. However, the question of what the marine fungi contributes to the plant and algae material biotransformation processes has yet to be highlighted sufficiently. In this review, we summarized the potential of marine fungi alternatively to terrestrial fungi to produce the biotechnologically valuable extracellular enzymes in response to the plant and macroalgae polymeric substrates as sources of carbon for their bioconversion used for industries and bioremediation. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
89. Process‐centric and data‐centric strategies for enhanced production of l‐asparaginase—an anticancer enzyme, using marine‐derived Aspergillus niger.
- Author
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Vala, Anjana K., Dudhagara, Dushyant R., and Dave, Bharti P.
- Subjects
- *
ASPARAGINASE , *ANTINEOPLASTIC agents , *ARTIFICIAL neural networks , *RESPONSE surfaces (Statistics) , *ASPERGILLUS niger - Abstract
Abstract: The objective of the study was to achieve enhanced production of l‐asparaginase (LA), an anticancer enzyme, by a marine‐derived Aspergillus niger isolate. To improve LA production, optimization of pH, incubation time, and inoculum size was performed using process‐centric (response surface methodology [RSM]) and data‐centric (artificial neural network [ANN]) approaches. The optimized conditions led to a 108.62% rise in LA production. Upon comparison of 2 models for enhanced LA production, based on the R2, mean absolute percentage error, root mean square error, and mean absolute deviation values, the ANN model was observed to be superior over the RSM model. To the authors' best knowledge, the present finding is the first ever report revealing detailed analyses for RSM and ANN models for LA production using a marine‐derived fungus. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
90. Production of pigments from the tropical marine-derived fungi Talaromyces albobiverticillius: New resources for natural red-colored metabolites.
- Author
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Venkatachalam, Mekala, Magalon, Hélène, Dufossé, Laurent, and Fouillaud, Mireille
- Subjects
- *
FILAMENTOUS fungi , *METABOLITES , *TALAROMYCES , *FERMENTATION , *PIGMENTS - Abstract
Filamentous fungi produce many chemical classes of metabolites bound to be used in various fields of industrial applications such as food, cosmetics, paper, textiles, medicine, biodegradation, waste remediation, etc. In this trend, scientists are actively looking for new fungal strains synthesizing original molecules with new potentialities. The present study is focused on pigment production from Talaromyces albobiverticillius strains, which were recently isolated from marine environments around Reunion Island, Indian Ocean. This study demonstrates their ability to produce a range of colored compounds exhibiting specific orange/red hues under submerged fermentation in potato dextrose broth (PDB), both seen in the mycelia and liquid medium. For the most promising strain, maximum levels of extracellular orange-yellow pigments (22.39 UA at 470 nm) and red pigments (18.67 UA at 500 nm) were attained in 8 days with an initial pH of 5.0 at 24 °C. Among the 12 components of the color hue, two compounds were produced in larger amounts in comparison with the total pigmented extracts (compound 4, red colored, presents two absorbance maxima: λ 1 = 422 nm, λ 2 = 511 nm; and compound 8, orange colored, absorbs at λ 1 = 469.8 nm as a maximum). The color characteristics of the extracellular fungal products measured by L*a*b* quantitative colorimetric system indicated variations between the three strains productions, as well as between the intracellular and extracellular colored molecules produced. An evolution of the colored compounds synthesized is thus highlighted, in relation with the growth phases of the fungus. These results provide information about the future directions towards large-scale cultivation of these fungi, which might support the industrial applications of the red pigments. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
91. Chemical constituents of the fermentative extracts of marine fungi Phoma sp. CZD-F11 and Aspergillus sp. CZD-F18 from Zhoushan Archipelago, China.
- Author
-
Wu, Xiaomei, Chen, Zhe, Ding, Wanjing, Liu, Yu, and Ma, Zhongjun
- Abstract
A new diphenyl ether (1) as well as 20 other compounds were identified from the fermentative extracts of marine-derived fungi Phoma sp. CZD-F11 (Compounds 1–8) and Aspergillus sp. CZD-F18 (Compounds 9–21). Their structures were elucidated on the basis of extensive spectroscopic analysis. The broth extracts of the fungi exhibited very good anticancer activity against H1975 cells with 5.62 and 25.8% viability at concentration of 10 μg/mL for Phoma sp. CZD-F11 and Aspergillus sp. CZD-F18, respectively. The inhibitory activity of all compounds against PC-3 cell lines, BRD4 and aromatase were evaluated. The results showed compound 7 exhibited moderate anticancer activity with 66.1% inhibition against PC-3 cell lines at the concentration of 10 μg/mL. Compound 7 and 8 exhibited favourable BRD4 inhibitory activity with 78.5 and 76.4% inhibition at the concentration of 10 μg/mL. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
92. Potential of Marine-Derived Fungi to Remove Hexavalent Chromium Pollutant from Culture Broth.
- Author
-
Lotlikar, Nikita P., Damare, Samir R., Meena, Ram Murti, Linsy, P., and Mascarenhas, Brenda
- Subjects
- *
ASPERGILLUS , *CHROMIUM removal (Water purification) , *MICROBIAL exopolysaccharides , *FUNGAL remediation , *BIOMASS production - Abstract
Chromium (Cr) released from industrial units such as tanneries, textile and electroplating industries is detrimental to the surrounding ecosystems and human health. The focus of the present study was to check the Cr(VI) removal efficiency by marine-derived fungi from liquid broth. Amongst the three Cr(VI) tolerant isolates, #NIOSN-SK56-S19 (
Aspergillus sydowii ) showed Cr-removal efficiency of 0.01 mg Cr mg−1 biomass resulting in 26% abatement of total Cr with just 2.8 mg of biomass produced during the growth in 300 ppm Cr(VI). Scanning Electron Microscopy revealed aggregation of mycelial biomass with exopolysaccharide, while Electron Dispersive Spectroscopy showed the presence of Cr2O3 inside the biomass indicating presence of active Cr(VI) removal mechanisms. This was further supported when the Cr(VI) removal was monitored using DPC (1,5-diphenylcarbazide) method. The results of this study point to the potential of marine-derived fungal isolates for Cr(VI) removal. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
93. Antibiotic activity of Emerimicin IV isolated from <italic>Emericellopsis minima</italic> from Talcahuano Bay, Chile.
- Author
-
Inostroza, Alejandro, Lara, Liliana, Paz, Cristian, Perez, Andrés, Galleguillos, Felipe, Hernandez, Victor, Becerra, José, González-Rocha, Gerardo, and Silva, Mario
- Abstract
Due to the increasing emergence of resistance of bacterial pathogens to current antibiotics, we have examined the marine fungi present in sea sediments obtained 200 m offshore to discover new antibacterial compounds active against multidrug-resistant bacteria. One strain, identified as
Emericellopsis minima , was isolated from sediments of Talcahuano Bay (Chile). From the liquid culture ofE. minima , we isolated Emerimicin IV, a unique fungal peptaibol that exhibited antibacterial activity. The structure of this compound was assigned by interpretation of1 H NMR and HR-LCMS data. Emerimicin IV showed bacteriostatic activity against clinical isolates of methicillin-resistantStaphylococcus aureus and vancomycin-resistantEnterococcus faecalis with MIC values ranging between 100 and 12.5 μg/mL. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
94. Isolation and X-ray structure analysis of citreohybridonol from marine-derived Penicillium atrovenetum.
- Author
-
Özkaya, Ferhat Can, Ebrahim, Weaam, Klopotowski, Maximilian, Liu, Zhen, Janiak, Christoph, and Proksch, Peter
- Abstract
The anti-neuroinflammatory meroterpenoid citreohybridonol was isolated for the first time from a sponge-derived fungusPenicillium atrovenetum. In this study, in addition to isolation and structure featuring, its unambiguous absolute configuration was determined exclusively by single crystal X-ray diffraction. The C-17-keto tautomer was clearly observed in X-ray analysis. The substance crystallises in the monoclinic space groupP21witha = 10.7496(5) Å,b = 14.3286(7) Å,c = 17.4909(8) Å,β = 103.235(2)°,V = 2622.5(2) Å3,Z = 2,Dcalcd = 1.280 g/cm3. The chirality of the asymmetric carbon atoms was as follows: C3 (S), C5 (R), C6 (S), C8 (S), C9 (R), C10 (R), C13 (R), C14 (R). [ABSTRACT FROM PUBLISHER]
- Published
- 2018
- Full Text
- View/download PDF
95. Optimization of culture conditions for penicilazaphilone C production by a marine-derived fungus Penicillium sclerotiorum M-22.
- Author
-
Zhao, H. -G., Wang, M., Lin, Y. -Y., and Zhou, S. -L.
- Subjects
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PENICILLIUM , *MARINE microbiology , *FUNGAL cultures , *ANTIBACTERIAL agents , *RESPONSE surfaces (Statistics) - Abstract
The aim of this study was to optimize the culture conditions of a marinederived fungus Penicillium sclerotiorum M-22 for the production of penicilazaphilone C (PAC), a novel azaphilonidal derivative exhibiting broad cytotoxic and antibacterial effects. By single factor experiments, the effects to the production of PAC of aged seawater concentration, initial pH values, fermentation time, carbon sources, nitrogen sources and inorganic salt sources were investigated individually. Response surface methodology (RSM) analysis was adopted to investigate the interactions between variables and determine the optimal values for maximum PAC production. Evaluation of the experimental results signified that the optimum conditions for maximum production of PAC (19·85 mg l -11) in 250 ml Erlenmeyer flask were fermentation time 24·83 days, pH of 7·00, corn meal concentration of 10·72 g l -11, yeast extract concentration of 4·58 g l -11, crude sea salt concentration of 20·59 g l -11. Production under optimized conditions increased to 1·344-fold comparing to its production prior to optimization. The higher PAC production and the penicilazaphilone C - producing marine fungus would be provide a promising alterative approach for industrial and commercial applications. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
96. Characterization of L-asparaginase from marine-derived Aspergillus niger AKV-MKBU, its antiproliferative activity and bench scale production using industrial waste.
- Author
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Vala, Anjana K., Sachaniya, Bhumi, Dudhagara, Dushyant, Panseriya, Haresh Z., Gosai, Haren, Rawal, Rakesh, and Dave, Bharti P.
- Subjects
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ASPARAGINASE , *ASPERGILLUS niger , *INDUSTRIAL wastes , *ANTINEOPLASTIC agents , *LYMPHOBLASTIC leukemia in children , *LEUKEMIA treatment - Abstract
L-asparaginase (LA), an enzyme with anticancer activities, produced by marine-derived Aspergillus niger was subjected to purification and characterization. The purified enzyme was observed to have molecular weight ∼90KDa. The enzyme retained activity over a wide range of pH, i.e. pH 4–10. The enzyme was quite stable in temperature range 20–40 °C. Tween 80 and Triton X-100 were observed to enhance LA activity while inhibition of LA activity was observed in presence of heavy metals. The values for K m was found to be 0.8141 mM and V max was 6.228 μM/mg/min. The enzyme exhibited noteworthy antiproliferative activity against various cancer cell lines tested. Successful bench scale production (in 5L bioreacator) of LA using groundnut oil cake as low cost substrate has also been carried out. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
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97. Two new tricycloalternarene acids from the marine-derived fungus Alternaria alternata ICD5-11.
- Author
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Fang, Sheng-Tao, Miao, Feng-Ping, Liu, Xiang-Hong, Song, Yin-Ping, and Ji, Nai-Yun
- Abstract
Two new meroterpenoids, named tricycloalternarenes K ( 1 ) and L ( 2 ), together with one known analog ( 3 ) were isolated from the marine-derived fungus Alternaria alternata ICD5-11, which was obtained from marine isopod Ligia exotica . Their structures and relative/absolute configurations were elucidated by extensive spectroscopic methods, including 1D/2D NMR, CD, and mass spectra and by comparison with literature data. The antimicrobial activities of two new compounds were also tested. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
98. Isolation and structure determination of a new diketopiperazine dimer from marine-derived fungus Aspergillus sp. SF-5280.
- Author
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Cho, Kwang-Ho, Sohn, Jae Hak, and Oh, Hyuncheol
- Abstract
A new diketopiperazine dimer designated as SF5280-415 (1) was isolated from an EtOAc extract of the marine-derived fungusAspergillussp. SF-5280 by various chromatographic methods. The structure of1was mainly determined by analysis of the NMR spectroscopic data and MS data, along with Marfey’s method. This compound is a new diastereoisomer of known bispyrrolidinoindoline diketopiperazine alkaloid WIN 64745, which possesses unique architecture biosynthetically derived from an indole oxidation reaction of tryptophan. [ABSTRACT FROM PUBLISHER]
- Published
- 2018
- Full Text
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99. De novo transcriptome assembly: a new laccase multigene family from the marine-derived basidiomycete Peniophora sp. CBMAI 1063.
- Author
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Otero, Igor, Ferro, Milene, Bacci, Maurício, Ferreira, Henrique, and Sette, Lara
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LACCASE , *CHEMICAL reactions , *PHENOLS , *AMINES , *BASIDIOMYCETES - Abstract
Laccases are multicopper oxidases that are able to catalyze reactions involving a range of substrates, including phenols and amines, and this ability is related to the existence of different laccases. Basidiomycetes usually have more than one gene for laccase, but until now, this feature has not been demonstrated in a marine-derived fungus. Peniophora sp. CBMAI 1063 is a basidiomycete fungus isolated from a marine sponge that exhibits the ability to secrete significant amounts of laccase in saline conditions. In the present study, we identified laccase sequences from the transcriptome of Peniophora sp. CBMAI 1063 and used them to perform different molecular in silico analyses. The results revealed the presence of at least eight putative genes, which may encode ten different laccases with peptide lengths ranging from 482 to 588 aa and molecular weights ranging from 53.5 to 64.4 kDa. These laccases seem to perform extracellular activities, with the exception of one that may represent an intracellular laccase. The 10 predicted laccases expressed by Peniophora sp. CBMAI 1063 in laccase-induced media showed different patterns of N-glycosylation and isoelectric points and are divided into two classes based on the residue associated with the regulation of the redox potential of the enzyme. None of the predicted laccases showed more than 61% similarity to other fungal laccases. Based on the differences among the laccases expressed by Peniophora sp. CBMAI 1063, this marine-derived basidiomycete represents a valuable resource with strong potential for biotechnological exploitation. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
100. New cytotoxic furan from the marine sediment-derived fungi Aspergillus niger.
- Author
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Uchoa, Paula Karina S., Pimenta, Antonia T. A., Braz-Filho, Raimundo, de Oliveira, Maria da Conceição F., Saraiva, Natália N., Rodrigues, Barbara S. F., Pfenning, Ludwig H., Abreu, Lucas M., Wilke, Diego V., Florêncio, Katharine G. D., and Lima, Mary Anne S.
- Abstract
A fungal strain ofAspergillus nigerwas recovered from sediments collected in the Northeast coast of Brazil (Pecém’s offshore port terminal). Cultivation in different growth media yielded a new ester furan derivative,1, along with malformin A1, malformin C, cyclo (trans-4-hydroxy-L-Pro-L-Leu), cyclo (trans-4-hydroxy-L-Pro-L-Phe), cyclo (L-Pro-L-Leu), cyclo (L-Pro-L-Phe), pseurotin D, pseurotin A, chlovalicin, cyclo (L-Pro-L-Tyr) and cyclo (L-Pro-L-Val). Compound1was cytotoxic against HCT-116 cell line, showing IC50 = 2.9 μg/mL (CI 95% from 1.8 to 4.7 μg/mL). [ABSTRACT FROM PUBLISHER]
- Published
- 2017
- Full Text
- View/download PDF
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