51. Degradation of CL-20 by white-rot fungi
- Author
-
Diane Fournier, Fanny Monteil-Rivera, Annamaria Halasz, Manish Bhatt, and Jalal Hawari
- Subjects
Environmental Engineering ,Time Factors ,Health, Toxicology and Mutagenesis ,Irpex lacteus ,Nitrous Oxide ,Phanerochaete ,environmental ,Gas Chromatography-Mass Spectrometry ,chemistry.chemical_compound ,Manganese peroxidase ,Heterocyclic Compounds ,Cations ,Environmental Chemistry ,Soil Pollutants ,Carbon Radioisotopes ,Nitrite ,Nitrites ,Nitrobenzenes ,Chrysosporium ,Aza Compounds ,Aniline Compounds ,biology ,Chemistry ,Public Health, Environmental and Occupational Health ,Environmental engineering ,General Medicine ,General Chemistry ,Biodegradation ,biology.organism_classification ,Pollution ,Decomposition ,Biodegradation, Environmental ,Peroxidases ,Anaerobic bacteria ,Nuclear chemistry - Abstract
In previous studies, we found that the emerging energetic chemical, CL-20 (C6H6N12O12, 2,4,6,8,10,12-hexanitro-2,4,6,8,10,12-hexaazaisowurtzitane), can be degraded following its initial denitration using both aerobic and anaerobic bacteria. The C and N mass balances were not determined due to the absence of labeled starting compounds. The present study describes the degradation of the emerging contaminant by Phanerochaete chrysosporium using ring-labeled [15N]-CL-20 and [14C]-CL-20. Ligninolytic cultures degraded CL-20 with the release of nitrous oxide (N2O) in amounts corresponding to 45% of the nitrogen content of CL-20. When ring-labeled [15N]-CL-20 was used, both 14N14NO and 15N14NO were observed, likely produced from -NO2 and N-NO2, respectively. The incubation of uniformly labeled [14C]-CL-20 with fungi led to the production of 14CO2 (>80%). Another ligninolytic fungus, Irpex lacteus, was also able to degrade CL-20, but as for P. chrysosporium, no early intermediates were observed. When CL-20 was incubated with manganese peroxidase (MnP), we detected an intermediate with a [M-H]- mass ion at 345 Da (or 351 and 349 Da when using ring-labeled and nitro-labeled [15N]-CL-20, respectively) matching a molecular formula of C6H6N10O8. The intermediate was thus tentatively identified as a doubly denitrated CL-20 product. The concomitant release of nitrite ions (Click to view the MathML source) with CL-20 degradation by MnP also supported the occurrence of an initial denitration prior to cleavage and decomposition.Keywords: CL-20; Degradation; Nitramine; Phanerochaete chrysosporium; Fungi
- Published
- 2005