71 results on '"Madoka Kuramitsu"'
Search Results
52. Induction of indistinguishable gene expression patterns in rats by Vero cell-derived and mouse brain-derived Japanese encephalitis vaccines
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Haruka Momose, Jun-ichi Imai, Isao Hamaguchi, Mika Kawamura, Takuo Mizukami, Seishiro Naito, Atsuko Masumi, Jun-ichi Maeyama, Kazuya Takizawa, Madoka Kuramitsu, Nobuo Nomura, Shinya Watanabe, and Kazunari Yamaguchi
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Microbiology (medical) ,Male ,Japanese Encephalitis Vaccines ,Gene Expression Profiling ,Body Weight ,Brain ,General Medicine ,Rats ,Mice ,Infectious Diseases ,Liver ,Encephalitis Viruses, Japanese ,Chlorocebus aethiops ,Animals ,Rats, Wistar ,Encephalitis, Japanese ,Vero Cells ,Blood Chemical Analysis ,Oligonucleotide Array Sequence Analysis - Abstract
Transcriptomics is an objective index that reflects the overall condition of cells or tissues, and transcriptome technology, such as DNA microarray analysis, is now being introduced for the quality control of medical products. In this study, we applied DNA microarray analysis to evaluate the character of Japanese encephalitis (JE) vaccines. When administered into rat peritoneum, Vero cell-derived and mouse brain-derived JE vaccines induced similar gene expression patterns in liver and brain. Body weights and blood biochemical findings were also similar after administration of the two vaccines. Our results suggest that the two JE vaccines are likely to have equivalent characteristics with regard to reactivity in rats.
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- 2010
53. Interferon regulatory factor-2 induces megakaryopoiesis in mouse bone marrow hematopoietic cells
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Seishiro Naito, Takuo Mizukami, Kazuya Takizawa, Kazunari Yamaguchi, Atsuko Masumi, Haruka Momose, Isao Hamaguchi, and Madoka Kuramitsu
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Platelet Membrane Glycoprotein IIb ,Transcriptional Activation ,Interferon Regulatory Factor 2 ,Biophysics ,Biology ,Biochemistry ,Cell Line ,Megakaryopoiesis ,Interferon-gamma ,Mice ,Structural Biology ,Bone Marrow ,Genetics ,medicine ,Animals ,Humans ,Clonogenic assay ,Promoter Regions, Genetic ,Molecular Biology ,IRF-2 ,Inflammation ,Stem cell ,Hematopoietic stem cell ,Cell Differentiation ,Cell Biology ,Hematopoietic Stem Cells ,Gene regulation ,Rats ,Mice, Inbred C57BL ,Haematopoiesis ,medicine.anatomical_structure ,Immunology ,Cancer research ,Bone marrow ,Megakaryocytes ,Interferon Regulatory Factor-2 ,Interferon regulatory factors - Abstract
Megakaryopoiesis is associated with inflammatory reactions. To investigate the role of interferon regulatory factors (IRFs) in inflammation-associated megakaryopoiesis, mouse bone marrow hematopoietic stem cells (HSCs) were analyzed. IFN-γ treatment induced IRF-2 expression as well as the expression of CD41 and IRF-1 in HSCs. An in vitro clonogenic assay showed that IRF-2- but not IRF-1-overexpressing cells increased the number of megakaryocytic colonies. IRF-2 transfection up-regulated CD41 promoter activity in hematopoietic cell lines. The number of CD41-positive bone marrow cells increased in mice injected with IRF-2-expressing bone marrow cells. These findings suggest that IRF-2 plays an important role in megakaryopoiesis in inflammatory states.
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- 2009
54. Identification of transcripts commonly expressed in both hematopoietic and germ-line stem cells
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Atsushi Iwama, Atsuko Masumi, Takuo Mizukami, Madoka Kuramitsu, Haruka Momose, Toshiaki Noce, Kazuya Takizawa, Isao Hamaguchi, Takehiko Ogawa, Kazunari Yamaguchi, and Seishiro Naito
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Male ,endocrine system ,Stem cell factor ,Biology ,Stem cell marker ,Mice ,Cancer stem cell ,Testis ,Animals ,Cell Lineage ,Tissue Distribution ,RNA, Messenger ,Progenitor cell ,Embryonic Stem Cells ,Gene Expression Profiling ,Stem Cells ,Amniotic stem cells ,Cell Biology ,Hematology ,Hematopoietic Stem Cells ,Embryonic stem cell ,Molecular biology ,Mice, Inbred C57BL ,Germ Cells ,Stem cell ,Developmental Biology ,Adult stem cell - Abstract
Germ-line stem cells (GSCs) constitute a stem cell population with remarkable stability and proliferative potential in vitro and are a useful model for studying the mechanism of self-renewal and "stemness" function of committed tissue stem cells. To identify GSC-specific genes, we performed subtractive hybridization using cDNA from GSCs, testis, and embryonic stem (ES) cells, and successfully identified 11 genes highly expressed in GSCs. Histological analysis confirmed expression of Cry alpha b, Mcpt8, Cxcl5, Fth1, Ctla2 alpha, and Spp1 in undifferentiated spermatogonia on the basement membrane area of the seminiferous epithelium of the testis, where the GSC niche is thought to be located. Among GSC-specific genes, quantitative PCR analysis showed seven genes-Fth1, Cry alpha b, Spp1, Bcap31, Arhgap1, Ctla2 alpha, and Serpina3g-to be common transcripts highly expressed in hematopoietic stem cells (HSCs). Histological analysis confirmed that Ctla2 alpha-, Serpina3g-, and Spp1-expressing cells were observed in the trabecular bone region of the bone marrow, where the HSC niche is located. Furthermore, histological analysis revealed that only Spp1 was expressed in the hair follicle bulge in the area of the hair follicle stem cell niche. Thus, identifying stemness genes by comparative analysis to GSCs is a powerful tool with which to explore the fundamental commonalities of HSCs and other stem cell types.
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- 2008
55. Deficient RPS19 protein production induces cell cycle arrest in erythroid progenitor cells
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Madoka Kuramitsu, Atsuko Masumi, Mizukami Takuo, Haruka Momose, Isao Hamaguchi, Kazuya Takizawa, Kazunari Yamaguchi, Seishirou Naito, and Masayo Mochizuki
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Ribosomal Proteins ,Blotting, Western ,Genetic Vectors ,Mutation, Missense ,Gene Expression ,Apoptosis ,Bone Marrow Cells ,Gene mutation ,Biology ,Cell Line ,Ribosomal protein S19 ,Humans ,Progenitor cell ,RNA, Small Interfering ,Cells, Cultured ,Anemia, Diamond-Blackfan ,Cell Proliferation ,Genetics ,Erythroid Precursor Cells ,Cell growth ,Reverse Transcriptase Polymerase Chain Reaction ,Cell Cycle ,Hematology ,Cell cycle ,Flow Cytometry ,Cell biology ,Haematopoiesis ,Retroviridae ,Cell culture ,RNA Interference ,Stem cell - Abstract
The gene encoding ribosomal protein S19 (RPS19) is one of the responsible genes for Diamond-Blackfan anaemia (DBA), a congenital erythroblastopenia. Although haplo-insufficiency of RPS19 has been suggested to be the onset mechanism underlying the pathogenesis of DBA, the sequential mechanism has not been elucidated. In order to analyse the consequences of the missense mutation of RPS19 specific for DBA patients, we made mutated RPS19 expression vectors. Twelve C-terminally Flag-tagged missense mutants were exogenously expressed from retroviral vectors and analysed by Western blot analysis and flow cytometry. When these 12 mutants were expressed in the erythro-leukaemic cell lines K562 and human bone marrow CD34(+) cells, almost all of the mutant proteins (except for G120R) were unstable, and the levels of mutated RPS19 protein were significantly low. To address the effect of deficient RPS19 expression on cell proliferation, RPS19 was downregulated by siRNA. Repressive expression of RPS19 in human CD34(+) cells produced an elevated number of cells at G0 and induced erythroid progenitor-specific defects in BM cells. These results suggest that abnormal ribosomal biogenesis causes inadequate cell cycle arrest in haematopoietic progenitors, and that, subsequently, erythroid progenitors are specifically hampered. These in vitro phenotypes of genetically manipulated CD34(+) cells mimic DBA pathogenesis.
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- 2008
56. Identification of ATL stem cells in an ATL model HBZ transgenic mouse
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Isao Hamaguchi, Madoka Kuramitsu, Takuo Mizukami, Kazuya Takizawa, Wakako Kuribayashi, Kenji Sugata, Haruka Momose, and Atsushi Iwama
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Genetically modified mouse ,Cancer Research ,Genetics ,Identification (biology) ,Cell Biology ,Hematology ,Stem cell ,Biology ,Molecular Biology ,Cell biology - Published
- 2015
57. Establishment of a New Quality Control and Vaccine Safety Test for Influenza Vaccines and Adjuvants Using Gene Expression Profiling
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Isao Hamaguchi, Haruka Momose, Kumiko Araki, Atsuko Masumi, Keiko Furuhata, Kazuya Takizawa, Kazunari Yamaguchi, Takuo Mizukami, and Madoka Kuramitsu
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Genetic Markers ,Male ,Quality Control ,Branched DNA Signal Amplification Assay ,Science ,Adjuvants, Immunologic ,Gene expression ,Animals ,Humans ,Rats, Wistar ,Gene ,Multidisciplinary ,biology ,Gene Expression Profiling ,Viral Vaccine ,PSMB9 ,Virology ,Rats ,Up-Regulation ,Vaccines, Virosome ,Gene expression profiling ,Influenza Vaccines ,Immunology ,biology.protein ,Medicine ,IRF7 ,TAP2 ,Biomarker (medicine) ,Safety ,Research Article - Abstract
We have previously identified 17 biomarker genes which were upregulated by whole virion influenza vaccines, and reported that gene expression profiles of these biomarker genes had a good correlation with conventional animal safety tests checking body weight and leukocyte counts. In this study, we have shown that conventional animal tests showed varied and no dose-dependent results in serially diluted bulk materials of influenza HA vaccines. In contrast, dose dependency was clearly shown in the expression profiles of biomarker genes, demonstrating higher sensitivity of gene expression analysis than the current animal safety tests of influenza vaccines. The introduction of branched DNA based-concurrent expression analysis could simplify the complexity of multiple gene expression approach, and could shorten the test period from 7 days to 3 days. Furthermore, upregulation of 10 genes, Zbp1, Mx2, Irf7, Lgals9, Ifi47, Tapbp, Timp1, Trafd1, Psmb9, and Tap2, was seen upon virosomal-adjuvanted vaccine treatment, indicating that these biomarkers could be useful for the safety control of virosomal-adjuvanted vaccines. In summary, profiling biomarker gene expression could be a useful, rapid, and highly sensitive method of animal safety testing compared with conventional methods, and could be used to evaluate the safety of various types of influenza vaccines, including adjuvanted vaccine.
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- 2015
58. Loss of Tie2 receptor compromises embryonic stem cell-derived endothelial but not hematopoietic cell survival
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Tohru Morisada, Yuichi Oike, Daniel J. Dumont, Toshio Suda, Kyoko Murakami, Kazunari Yamaguchi, Mizukami Takuro, Masaki Azuma, Kazuyuki Ohbo, Madoka Kuramitsu, and Isao Hamaguchi
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Cell Survival ,Cellular differentiation ,Immunology ,Neovascularization, Physiologic ,Biochemistry ,Mice ,Lymph node stromal cell ,Animals ,Progenitor cell ,Cells, Cultured ,Interleukin 3 ,Glycoproteins ,CD40 ,biology ,Endothelial Cells ,Membrane Transport Proteins ,Cell Differentiation ,Cell Biology ,Hematology ,Embryo, Mammalian ,Hematopoietic Stem Cells ,Receptor, TIE-2 ,Cell biology ,Hematopoiesis ,Endothelial stem cell ,Platelet Endothelial Cell Adhesion Molecule-1 ,Stem Cells in Hematology ,surgical procedures, operative ,embryonic structures ,biology.protein ,cardiovascular system ,sense organs ,Stem cell ,tissues ,Adult stem cell ,Signal Transduction - Abstract
Tie2 is a receptor-type tyrosine kinase expressed on hematopoietic stem cells and endothelial cells. We used cultured embryonic stem (ES) cells to determine the function of Tie2 during early vascular development and hematopoiesis. Upon differentiation, the ES cell–derived Tie2+Flk1+ fraction was enriched for hematopoietic and endothelial progenitor cells. To investigate lymphatic differentiation, we used a monoclonal antibody against LYVE-1 and found that LYVE-1+ cells derived from Tie2+Flk1+ cells possessed various characteristics of lymphatic endothelial cells. To determine whether Tie2 played a role in this process, we analyzed differentiation of Tie2-/- ES cells. Although the initial numbers of LYVE-1+ and PECAM-1+ cells derived from Tie2-/- cells did not vary significantly, the number of both decreased dramatically upon extended culturing. Such decreases were rescued by treatment with a caspase inhibitor, suggesting that reductions were due to apoptosis as a consequence of a lack of Tie2 signaling. Interestingly, Tie2-/- ES cells did not show measurable defects in development of the hematopoietic system, suggesting that Tie2 is not essential for hematopoietic cell development.
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- 2005
59. The Osteoclast Targeting Therapy In Bone Metastasis For a Mouse Model Of Adult T Cell Leukemia
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Madoka Kuramitsu, Kazuya Takizawa, Kazunari Yamaguchi, Jumpei Yamazaki, Ryuji Hiramatsu, Hideki Hasegawa, Wakako Kuribayashi, Takuo Mizukami, William W. Hall, and Isao Hamaguchi
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Stromal cell ,Immunology ,T-cell leukemia ,Cell Biology ,Hematology ,Biology ,Biochemistry ,Transplantation ,medicine.anatomical_structure ,immune system diseases ,RANKL ,Reticular cell ,Osteoclast ,hemic and lymphatic diseases ,medicine ,biology.protein ,Cancer research ,Bone marrow ,Stem cell - Abstract
Adult T cell leukemia (ATL) is a lymphoproliferative disorder caused by infection with HTLV-I. Although various chemotherapies have shown significant complete remission rates, most of the treated patients relapse. These data indicate the existence of leukemic stem cells (LSCs) and a specific niche that regulates stemness and protects LSCs from various chemotherapies. We have reported in previous studies that the ATL-LSCs isolated from a Tax-transgenic (Tax-Tg) mouse are enriched in the CD117+/CD38–/CD71– fraction of the lymphoma, and LSCs have the potential to reproduce the original tumor when transplanted into a NOD/SCID mouse (Yamazaki et al., Blood, 2009). However, the niche of ATL-LSCs is still unclear. To identify the ATL-LSC niche in vivo, we performed a homing assay. Splenic lymphoma cells isolated from a Tax-Tg mouse were GFP transduced by a lentivirus, and then sorted GFP+ cells were transplanted intra-peritoneally into a non-irradiated NOD/SCID mouse. The homing of GFP+ cells to tissues was assessed by flow cytometry (FCM) at 16 hours and 3, 7, 14 and 21 days after transplantation. As a result, GFP+ lymphoma cells were first detected in the spleen and BM at 16 hours after transplantation. No GFP+ lymphoma cells were detected in the thymus and LN. Interestingly, more than 60% of first colonized cells in the spleen and BM at 16 hours after transplantation were AT-LSCs (GFP+/CD117+ cells). From day 3 to 7, more than 40% of colonizing cells in the BM and spleen were ATL-LSCs. To identify the specific niche of ATL-LSCs in the BM, we performed imaging analysis of ATL-LSCs. ATL-LSCs (GFP+/CD117+ and CD38–/CD71–/CD117+ cells) were mainly localized near the endosteal region of trabecular bone in the BM. We found that ATL-LSCs were also attached to the reticular cells in the trabecular bone. In addition, we found the number of osteoclast was significantly increased at the trabecular region. Increasing number of osteoclasts correlates the increased the serum calcium concentration and decreased the mass of trabecular bone. FCM analysis and in vitro differentiation assay confirmed that the number of osteoclast precursors was increased in the ATL BM. To clarify the role of osteoclast in the ATL BM, we treated osteoclast inhibitor Zoledronic acid (ZOL) to the ATL mouse model. As a result, ZOL itself significantly reduced the number of GFP+ ATL cells in the BM. When we treated ZOL with anti cancer drug, GFP+ ATL cells were dramatically reduced in the BM and extend the mouse survival rate significantly despite anti cancer drug does not reduced the number of ATL cells itself. In addition, abnormal trabecular bone morphology was completely recovered in the treated mouse. These data suggest that osteoclast may have a function to support leukemic stem cell niche. To clarify the key signals to induce osteoclast in ATL BM, we checked the expression of RANKL and PTHrP. We found that RANKL was up-regulated both in the lymphoma cell and stromal cells in the bone marrow. In this study, we found that ATL-LSC niche is located at the trabecular bone region in the BM and osteoclasts have a role to support ATL cell and develop LSCs niche in a mouse model of ATL. We conclude that osteoclast have a potential therapeutic target in the mouse model of ATL. Disclosures: No relevant conflicts of interest to declare.
- Published
- 2013
60. Identification of Leukemic Stem Cells and Their Niche in Adult T Cell Leukemia Using the Tax-Transgenic Mouse Model
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Jumpei Yamazaki, Takuo Mizukami, Haruka Momose, Hideki Hasegawa, William W. Hall, Madoka Kuramitsu, Kazuya Takizawa, Kazunari Yamaguchi, Atsuko Masumi, and Isao Hamaguchi
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Immunology ,Mesenchymal stem cell ,Spleen ,Cell Biology ,Hematology ,Biology ,CD38 ,Biochemistry ,Molecular biology ,medicine.anatomical_structure ,immune system diseases ,Reticular cell ,hemic and lymphatic diseases ,medicine ,Bone marrow ,Stem cell ,Lymph node ,Homing (hematopoietic) - Abstract
Abstract 1877 Adult T cell leukemia (ATL) is a lymphoproliferative disorder caused by infection with HTLV-I. Although various chemotherapies have shown significant complete remission rates, most of the treated patients relapse. These data indicate the existence of leukemic stem cells (LSCs) and a specific niche that regulates stemness and protects these cells from chemotherapy. We have reported in previous studies that the ATL-LSCs isolated from a Tax-transgenic (Tax-Tg) mouse are enriched in the CD117+/CD38–/CD71– fraction of the lymphoma, and LSCs have the potential to reproduce the original tumor when transplanted into a NOD/SCID mouse (Yamazaki et al., Blood, 2009). However, the niche of ATL-LSCs in the spleen, bone marrow (BM), thymus and lymph node (LN) is still unclear. To identify the ATL-LSC niche in vivo, we performed a homing assay. Lymphoma cells isolated from a Tax-Tg mouse were GFP transduced by a lentivirus, and then sorted GFP+ cells (2×106) were transplanted intraperitoneally into a non-irradiated NOD/SCID mouse. The homing of GFP+ cells to tissues was traced by flow cytometry (FCM) at 16 hours and 3, 7, 14 and 21 days after transplantation. At 16 hours after transplantation, GFP+ lymphoma cells were detected in the spleen and BM. No GFP+ lymphoma cells were detected in the thymus and LN. Interestingly, more than 60% of first colonized cells in the spleen and BM at 16 hours were AT-LSCs (GFP+/CD117+ cells). From day 3 to 7, more than 40% of proliferating cells in the BM and spleen were ATL-LSCs. At day 3, only a few non-ATL-LSCs (GFP+/CD117–cells) were detected in the thymus, LN and peripheral blood. The number of GFP+ cells was drastically increased at day 14 in the spleen. These data indicate that ATL-LSCs prefer to colonize and proliferate in the spleen and BM. To identify the specific niche of ATL-LSCs in the spleen and BM, we performed imaging analysis of ATL-LSCs. ATL-LSCs (GFP+/CD117+ and CD38–/CD71–/CD117+cells) were mainly localized near the vascular region in the spleen and endosteal region of trabecular bone in the BM. We found that some ATL-LSCs were attached to reticular cells (RC) in the spleen. In the BM, ATL-LSCs cells were localized at the endosteal region of the trabecular bone. Interestingly, similar to the spleen, RCs were observed at the endosteal region and contacted ATL-LSCs in the BM. FCM analysis confirmed that the number of reticular cells and mesenchymal stem cells (MSCs), were increased in the ATL BM and spleen. These data suggest that RCs are a possible candidate for the ATL-LSC niche and may be a new target of therapy. Finally, to characterize the ATL-LSC niche, we isolated osteoblastic cells, blood endothelial cells, lymphatic endothelial cells and reticular cells from normal and ATL BM to compare the gene expression profiles of each niche cell type. Here, together with DNA microarray analysis of ATL-LSCs both in the BM and spleen, we have characterized ATL-LSC niche cells both in the spleen and BM. Disclosures: No relevant conflicts of interest to declare.
- Published
- 2012
61. Identification of Two New DBA Genes, RPS27 and RPL27, by Whole-Exome Sequencing in Diamond-Blackfan Anemia Patients
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Kenichi Chiba, Kenichi Koike, Isamu Kamimaki, Seishi Ogawa, Akira Ishiguro, Kazuko Kudo, Isao Hamaguchi, Shouichi Ohga, Etsuro Ito, Seiji Kojima, Kenichi Yoshida, Kousaku Matsubara, Madoka Kuramitsu, Yuichi Shiraishi, RuNan Wang, Tomohiko Sato, Yusuke Okuno, Yuji Iribe, Hitoshi Kanno, Aiko Sato-Otsubo, Tsutomu Toki, Yoshifumi Kawano, Kanji Sugita, Kiminori Terui, Junichi Hara, Satoru Miyano, and Akira Ohara
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Genetics ,Immunology ,Intron ,Cell Biology ,Hematology ,Biology ,medicine.disease ,Bioinformatics ,Biochemistry ,Frameshift mutation ,Germline mutation ,medicine ,Missense mutation ,Macrocytic anemia ,Diamond–Blackfan anemia ,Gene ,Exome sequencing - Abstract
Abstract 984 Diamond-Blackfan anemia (DBA) is a congenital bone marrow failure syndrome, characterized by red blood cell aplasia, macrocytic anemia, and increased risk of malignancy. Approximately 90% of patients present during the first year of life or in early childhood. About 40–50% of DBA cases are familial with autosomal dominant, while the remainder is sporadic cases whose mode of inheritance is largely unknown. Although anemia is the most prominent feature of DBA, up to 40% of patients also accompany other symptoms including growth retardation and/or a variety of congenital malformations. Recent studies have shown that the disease could be associated with heterozygous mutations in ribosomal protein (RP) genes, including six small subunit RP genes RPS7, RPS10, RPS17, RPS19, RPS24, and RPS26 as well as four large subunit RP genes RPL5, RPL11, RPL26, and RPL35A, which collectively account for about 50% of patients with DBA. In addition, germline mutations in the GATA1 gene encoding a hematopoietic transcription factor, have been also reported in two DBA families. However, it is clear that the molecular etiology of many DBA cases remains to be covered. To identify new mutations that are responsible for DBA, we performed whole-exome sequencing on 40 DBA patients with no documented mutations/deletions involving known DBA genes. After excluding all variants registered in the 1000 Genomes Project, or dbSNP131, or found in our inhouse SNP database, we searched for non-synonymous mutations involving RP genes as possible candidate for novel DBA genes. In this study, we identified probable pathogenic mutations in two novel RP genes, RPS27 and RPL27 in two patients. The first case was a 1-year-old girl who harbored a single nucleotide substitution at the splice acceptor site in intron 1 of RPL27 (c.-2–1G>A), which results in splicing error. She had atrial septal defect and pulmonary stenosis, and responded to steroid treatment. The second case was a 2-year-old girl carrying a frameshift deletion of RPS27 (c.90delC, p.Tyr31ThrfsX5), leading to a premature truncation. This patient had no abnormalities and responded to steroid treatment. An additional five missense SNVs affecting single cases was identified in five genes, including RPL3L, RPL8, RPL13, RPL18A, and RPL31, together with two in-frame deletions of RPL6 and RPL14 in two patients, which cause deletion of a single amino-acid. However, the pathological significance in these 7 cases is uncertain. In the remaining 31 patients, no mutations were detected in RP genes. In conclusion, we identified novel germline mutations of RP genes that could be responsible for DBA, further confirming the concept that the RP genes are common targets of germline mutations in DBA patients and also suggested the presence of non-RP gene targets for DNA. Disclosures: No relevant conflicts of interest to declare.
- Published
- 2012
62. P039 Interferon regulatory factor-2 regulates hematopoietic stem cell in mouse bone marrow
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Haruka Momose, Takuo Mizukami, Kazuya Takizawa, Kazunari Yamaguchi, Madoka Kuramitsu, Isao Hamaguchi, and Atsuko Masumi
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education.field_of_study ,Immunology ,Population ,Hematology ,Biology ,Biochemistry ,Molecular biology ,Transplantation ,Haematopoiesis ,medicine.anatomical_structure ,Bone marrow suppression ,Interferon ,medicine ,Immunology and Allergy ,Bone marrow ,Stem cell ,education ,Molecular Biology ,Whole Bone Marrow ,medicine.drug - Abstract
Introduction Interferon regulatory factor-2 (IRF-2) is a transcriptional repressor in the interferon system and is thought to function by competing with IRF-1. Its physiological role in lymphoid and hematopoietic development has been investigated in IRF-2−/− mice, in which a general bone marrow suppression of hematopoiesis and B lymphopoiesis has been reported. Methods We investigate the role of IRF-2 in mouse bone marrow hematopoietic stem cells using IRF-2−/− mice. Hematopoietic stem cells (as KSL) are isolated from IRF-2−/− mice bone marrow and transplanted to lethally ittadiated mice for chimerismanalysis. Results IRF-2 is strongly expressed in hematopoietic stem cells (HSC) in mouse bone marrow. The population of bone marrow Lin-c-Kit+Sca-1+(KSL) cells is increased in IRF-2−/− mice due to the general enhancement of Sca-1-positive cells. SCs are enriched in KSL CD150+CD48-cells. A very small population of CD150-positive cells was detected in bone marrow from IRF-2−/− mice. Real-time polymerase chain reaction showed reduced CD150 gene expression in bone marrow cells from these mice compared to wild type mice. Although the population of bone marrow KSL cells in IRF-2−/− mice increased, a reduction of HSC was observed due to a decrease of the CD150-positive cell population. In IRF-2−/− interferon-a receptor−/− double-knockout mice, the CD150 expression levels were comparable to wild-type mice, indicating that this gene is regulated by the type [1] interferon (IFN) response via transcriptional regulation. Transplantation of KSL cells from IRF-2−/− mice into wild-type mice together with a competitor resulted in failed to engraft. Even the transplantation of a >20-fold excess of KSL cells from IRF-2−/− mice resulted in engraftment that was still poorer than wild-type cells evaluated at 3–4 months after transplantation. However, transplantation of a higher dose (2 × 10 6 cells) of whole bone marrow mononuclear cells from IRF-2−/− mice rescued recipients from lethal irradiation, although the engraftment was poorer than wild-type cells. This documents the presence of some active long-term repopulating hematopoietic stem cells (LT-HSCs) in IRF-2−/− mice. Conclusion Our results reveal unknown HSC markers in IRF-2−/− mouse bone marrow and demonstrate that IRF-2 acts on LT-HSCs not only through protective type I IFN responses, but also by directly regulating HSC cell surface molecules.
- Published
- 2012
63. New Determination Method for Extensive Gene Deletions In Diamond–Blackfan Anemia
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Tomohiro Morio, Tsutomu Toki, Takuo Mizukami, Kazuya Takizawa, Kazunari Yamaguchi, Madoka Kuramitsu, Atsuko Masumi, Etsuro Ito, Kiminori Terui, Haruka Momose, Masatoshi Takagi, RuNan Wang, and Isao Hamaguchi
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Genetics ,Point mutation ,Immunology ,Nonsense mutation ,Cell Biology ,Hematology ,Biology ,Biochemistry ,Exon ,Ribosomal protein ,Copy-number variation ,Allele ,Primer (molecular biology) ,Gene - Abstract
Abstract 4231 Introduction: Fifty percent of Diamond–Blackfan anemia (DBA) patients possess mutations in ribosomal protein genes. Although several ribosomal protein genes, RPL5, RPL11, RPL35A, RPS7, RPS10, RPS17, RPS19, RPS24, and RPS26, have been reported to be mutated in some DBA patients, including point mutations, nonsense mutations, deletions, splice site mutations, and translocations, other DBA patients appear to have intact ribosomal protein genes. To identify new mutations in ribosomal protein genes from a different aspect, we focused on extensive deletions in these genes, such as mutations involving loss of a whole allele. In this study, we applied quantitative genomic PCR, and successfully developed a convenient method for detecting extensive deletions designated the “DBA gene copy number assay”. Methods: DBA patients should have an intact allele and a mutated allele for the responsible ribosomal protein gene, meaning that they will have an abnormal karyotype (gene copy number of N) if they have an extensive deletion. We attempted to clarify the copy numbers of ribosomal protein genes by the difference in a 1-cycle delay of threshold in a quantitative PCR (q-PCR) assay. To detect extensive deletions, at least 2 sets of gene-specific primers for each DBA responsible gene (RPL5, RPL11, RPL35A, RPS7, RPS10, RPS17, RPS19, RPS24, and RPS26) were prepared. Appropriate primers to fit the setting that the threshold cycle (Ct) of the q-PCR should occur within 1 cycle of the Ct scores of other primer sets were selected. After validation, we identified 6, 3, 4, 3, 3, 6, 9, 3, and 2 specific primer sets for RPL5, RPL11, RPL35A, RPS7, RPS10, RPS17, RPS19, RPS24, and RPS26, respectively. By simply looking at the q-PCR amplification curves by eye, we were easily able to judge the copy numbers of 2N (normal) or N (abnormal) for the ribosomal protein genes. Results: We performed the DBA gene copy number assay for 14 randomly selected undiagnosed patients from the Japanese DBA genomic resource at the University of Hirosaki, who had no mutations by genomic sequencing analyses. For each case, all the DBA responsible genes were confirmed using the diagnostic primers. The results of the DBA gene copy number assays revealed that 5 of the 14 probands (36%) had an extensive deletion in one of the DBA responsible genes. As an interesting case among the 5 positive cases, we confirmed an extensive deletion in the RPS19 gene. The Ct scores for 4 of the 9 primer sets for RPS19 demonstrated a 1-cycle delay, while the scores for the other 5 primer sets were normal. By genomic PCR amplification analyses, we identified a deletion from nt. -1400 to +5757 (7157 nucleotides) in the RPS19 gene. The deleted region included the promoter region, and exons 1, 2, and 3 of the RPS19 gene. The remaining 4 cases were 1 proband with an RPL5 deletion, 1 with an RPL35A deletion and 2 with RPS17 deletions. In particular, the extensive deletions in the RPL5 and RPS17 alleles are the first such cases reported. Discussion: Since it has been difficult to address the loss of a whole allele in DBA, such mutations have not been precisely examined within the DBA responsible genes. Our data suggest that extensive deletions in ribosomal protein genes comprise a significant proportion of DBA cases in Japan. Our novel method could become a useful tool for screening the gene copy numbers of ribosomal protein genes, and for identifying new pathological mutations. Disclosures: No relevant conflicts of interest to declare.
- Published
- 2010
64. Furin-dependent CCL17-fused recombinant toxin controls HTLV-1 infection by targeting and eliminating infected CCR4-expressing cells in vitro and in vivo.
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Masateru Hiyoshi, Kazu Okuma, Seiji Tateyama, Kazuya Takizawa, Masumichi Saito, Madoka Kuramitsu, Kumiko Araki, Kazuhiro Morishita, Seiji Okada, Naoki Yamamoto, Arya Biragyn, Kazunari Yamaguchi, and Isao Hamaguchi
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HTLV-I ,ADULT T-cell leukemia ,PSEUDOMONAS ,PROPROTEIN convertases ,CELL lines ,CHEMOKINES - Abstract
Background: Adult T-cell leukemia (ATL) is caused by human T-cell leukemia virus type 1 (HTLV-1) infection. However, there are no therapies to prevent ATL development in high-risk asymptomatic carriers. To develop a therapy targeting HTLV-1-infected cells that are known to express CCR4 frequently, we tested whether truncated Pseudomonas exotoxin (PE38) fused to a CCR4 ligand, CCL17/thymus and activation-regulated chemokine (TARC), selectively eliminates such cells. Results: Our data show that TARC-PE38 efficiently killed HTLV-1-infected cell lines. It also shrank HTLV-1-associated solid tumors in an infected-cell-engrafted mouse model. In HTLV-1-positive humanized mice, TARC-PE38 markedly inhibited the proliferation of HTLV-1-infected human CD4
+ CD25+ or CD4+ CD25+ CCR4+ cells and reduced the proviral loads (PVLs) in peripheral blood mononuclear cells (PBMCs). Importantly, TARC-PE38 significantly reduced the PVLs in PBMCs obtained from asymptomatic carriers. We show that the cytotoxicity of TARC-PE38 is mediated by the expression of the proprotein convertase, furin. The expression of furin was enhanced in HTLV-1-infected cells and correlated positively with PVLs in HTLV-1-infected individuals, suggesting that infected cells are more susceptible to TARC-PE38 than normal cells. Conclusions: TARC-PE38 robustly controls HTLV-1 infection by eliminating infected cells in both a CCR4- and furindependent manner, indicating the excellent therapeutic potential of TARC-PE38. [ABSTRACT FROM AUTHOR]- Published
- 2015
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65. Identification and Characterization of a Hematopoietic Stem Cell Niche in Spleen
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Haruka Momose, Isao Hamaguchi, Seiji Okada, Madoka Kuramitsu, Atsuko Masumi, Seishiro Naito, Takuo Mizukami, Kazuya Takizawa, and Kazunari Yamaguchi
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Pathology ,medicine.medical_specialty ,Hematopoietic stem cell niche ,Immunology ,CD34 ,Spleen ,Cell Biology ,Hematology ,Biology ,Biochemistry ,Embryonic stem cell ,Cell biology ,Haematopoiesis ,medicine.anatomical_structure ,Megakaryocyte ,medicine ,Bone marrow ,Stem cell - Abstract
In normal hematopoiesis, hematopoietic stem cells (HSCs) in bone marrow mainly produce various types of blood cells. HSCs have a function of self-renewal and are located in the osteoblastic and vascular niche, which regulates the stemness function as a stem cell microenvironment. Recent studies clearly show that several factors (N-Cadherin, b-Catenin, SDF-1a and Spp1) are involved in the maintenance of the stemness function in HSCs. However, it has also been shown that embryonic HSCs can proliferate and differentiate in extrameduller tissues like liver, spleen and placenta. In adults, extrameduller hematopoiesis (EMH) can be induced by a hematological malignancy and some infectious diseases. However, the mechanism of HSCs regulation and niche cells at the EMH has not been well defined. To reveal the mechanism of HSCs in the EMH, we utilized the c-fos knockout mouse (c-fos −/−) as an EMH model mouse. In c-fos −/−, hematopoiesis in bone marrow was absent as a result of marrow spaces being occupied by increasing the number of bone forming osteoblasts; EMH then began in the spleen. First, to identify the main site of the HSC-niche in the EMH spleen, we performed HSC localization analysis using in situ hybridization of various HSC markers. Surprisingly, some CD34, Sca-1, c-kit, SCL/tal-1, and Tie-2 expressing cells were located near megakaryocyte like cells (MLCs). These MLCs were mainly located in the red pulp region in the spleen, and 3–5 cells form a syncytium. Interestingly, these MLCs express various types of osteoblastic niche related molecules, (N-Cadherin, b-Catenin, Spp1 and SDF-1a) in addition to megakaryocytic markers (CD41, CD61, and b3-integrin), suggesting that MLC is a HSC niche candidate in the EMH. To confirm our hypothesis, we next performed CFU-S (colony forming unit-spleen) assay as a naturally induced EMH model. Total 1×105 bone marrow mononuclear cells isolated from Ly5.2 or GFP-transgenic mouse were transplanted into lethally irradiated Ly5.2 mouse. In this system, MLCs were first seen in the spleen at day 1. At day 8, the number of MLCs had increased and units of 10–15 MLCs aggregated and formed syncytium. About 80% of MLCs were derived from donor (Ly5.2) cells. More interestingly, and contrary to our expectations, these aggregated MLCs with Sca-1+ or c-kit+ hematopoietic progenitor cells (HPCs) were mainly located, not inside the colony, but in the interstitial region between the developing colonies. They also expressed osteoblastic niche molecules. It has been suggested that each colonies in spleen are derived from HPCs and that HPCs exist inside the colony. Our data indicate the possibility that HPCs were transiently- located outside the colony. To undertake a detailed characterization of MLCs, we sorted donor derived MLCs as a Lin-/CD41+ cells and performed Q-PCR analysis. In agreement with our histological analysis, the sorted MLCs expressed various types of niche molecules and cytokines, compared to megakaryocytes. As well, when we co-cultured HSCs with isolated MLCs, the numbers of HSC were significantly increased compare to with a liquid culture system. Taking these data together, we suggested that MLCs have the potential to support HSC proliferation. In this study, we first identified a candidate for EMH-niche cells and postulated a developmental mechanism in the spleen. Our findings provide a new functional insight into HSCs outside the bone marrow, and extend a new tool that supports ex vivo expansion of HSCs.
- Published
- 2008
66. 206 The role for interferon regulatory factor-2 on megakaryopoiesis mediated by IFN-γ induction
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Isao Hamaguchi, Takuo Mizukami, Kazuya Takizawa, Kazunari Yamaguchi, Seishirou Naito, Haruka Momose, Madoka Kuramitsu, and Atsuko Masumi
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Chemistry ,Interferon Regulatory Factor 2 ,Immunology ,Cancer research ,Immunology and Allergy ,Hematology ,Molecular Biology ,Biochemistry ,Megakaryopoiesis - Published
- 2008
67. Spp1 (Osteopntin) Is Essential for the Early Niche Formation in the Bone Marrow
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Haruka Momose, Kazuya Takizawa, Kazunari Yamaguchi, Takuo Mizukami, Isao Hamaguchi, Seiji Okada, Toshiaki Noce, and Madoka Kuramitsu
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Pathology ,medicine.medical_specialty ,Immunology ,CD34 ,Osteoblast ,Cell Biology ,Hematology ,In situ hybridization ,Biology ,Biochemistry ,Molecular biology ,Transplantation ,Haematopoiesis ,medicine.anatomical_structure ,medicine ,Bone marrow ,Stem cell ,Adult stem cell - Abstract
Hematopoietic stem cells (HSCs) have a potential to differentiate into variety types of blood cells, and have an ability of self-renewing in the bone marrow (BM). All these fates are regulated by intrinsic genetic pathways in the micro-enviroment (niche) where HSCs are located. Osteoblasts are thought to be the hematopoietic niche which regulates HSC function in the BM. To clarify the molecular basis of hematopoietic niche system, we compared the two somatic stem cells (Germ line stem cell (GS) and HSC) using cDNA suppression subtractive hybridization, and identified 12 stem cell-specific genes which are expressed both in the HSCs and GS. Among these genes, we analyzed Spp1, since it is a major glycoprotein produced by osteoblast and it has been reported to be a negative regulator of hematopoietic niche size in the BM. In situ Hybridization (ISH) with Spp1-specific probes demonstrated that Spp1-expressing osteobalsts were located at the endosteal region of trabecular bone (TB). In the TB of adult mouce, Spp1-expressing hematopoietic cells attached with Spp1-expressing osteobalsts. Double ISH analysis revealed that Spp1 and Tie2 were partially co-expressed in the TB. These data suggests that Spp1 is an important molecule of hematopoietic niche system which were produced and interacted both HSCs and niche cell. When we analyzed spp1 knock out mouse (Spp1−/−), adult Spp1−/− mice (8 weeks) appeared to be normal and fertile. However, the abnormal TB formation was observed in the younger Spp1−/− mice (2 weeks). A number of osteobalsts were increased and formed a multi-layer of osteoblasts at the endosteal region of TB. Along with the morphological change of the TB, distribution pattern of CD34, Tie2 and Bmi-1 expressing HSC was unusual. Tie2 expressing cells of Spp1−/− mouse were mainly located at vascular-enriched zone in the bone marrow, but few Tie2 expressing cells at osteoblastic zone. These data suggest that HSC and osteobalst do not form the correct niche in the Spp1−/− TB, and Spp1 is thought to be essential for the initial niche formation. To confirm the function of Spp1 at the initiation phase of niche formation, we performed the bone marrow transplantation. Mononuclear cells (1×105 cells) from Ly5.1 mice were transplanted into lethally irradiated Ly5.2 mice. At 12 days after transplantation, donor hematopoietic cells were observed in the recipient TB. At this day 12, the expression of Spp1 was up-regulated in the endosteal region of TB, however, Spp1 expression was decreased in the course of reconstitution. Thus, the findings regarding the hematopoietic niche formation in Spp1−/− mice and in the irradiated mice allow to understand the Spp1 function in the initial phase of niche formation in the bone marrow.
- Published
- 2006
68. Analysis on the Correlation of RPS19 Expression Level to Diamond-Blackfan Anemia; Insights from the Expression Level of RPS19 Point Mutants in Erythroid Cell Lines
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Kazuya Takizawa, Kazunari Yamaguchi, Masayo Motizuki, Haruka Momose, Seishirou Naito, Atsuko Masumi, Mizukami Takuo, Madoka Kuramitsu, and Isao Hamaguchi
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Point mutation ,Immunology ,HEK 293 cells ,Mutant ,Cell Biology ,Hematology ,Cell cycle ,Biology ,medicine.disease ,Biochemistry ,Molecular biology ,Cell culture ,Ribosomal protein S19 ,medicine ,Diamond–Blackfan anemia ,Gene - Abstract
Mutations in ribosomal protein S19 (RPS19) gene is closely related to Diamond-Blackfan anemia (DBA). We have found point mutation, deletion and insertion in RPS19 gene in DBA patients (Blood, 100: 2724–31, 2002). In our analysis of DBA patients, gene expression of RPS19 from these mutated genomes were down regulated. Retroviral transduction of RPS19 gene to patient bone marrow cells restored the ability to differentiate into erythroid in vitro (Mol Ther., 7: 613–22, 2003). However, the mechanisms of how these mutations in RPS19 associate with anemia remains unknown. To analyze the mutated genome, we have made constructs of mutated RPS19 gene-expression vectors. From reported 56 different RPS19 mutations, we focused on the missense mutants and tried to characterize them. Flag-tagged twelve missense mutants (V15F, L18R, P47L, W52R, R56Q, S59F, A61Q, R62W, R101H, G120R, G127Q, and G131R) were exogenously expressed in several cell lines by retroviral vectors and were analysed by western blotting or FACS. When these 12 mutants were expressed in erythro-leukemic cell lines K562 and HEL, almost all the mutant proteins (except for G120R) were expressed at significantly low level compared to that of wild type. However, some of these mutants were expressed in human kidney epithelial cell line, 293T cells. These findings suggest that the expression level of RPS19 protein derived from mutated RPS19 genome is dependent on cell types, whether erythroid-lineage cells or others. In order to analyze the function of RPS19, doxycycline (Dox)-inducible expression of siRNA against RPS19 was induced in K562 cells and observed growth arrest at day 4 after induction of siRNA by Dox. Expression level of RPS19 decreased to one tenth of the normal level. Cell cycle analysis and quantitative-PCR analysis revealed that the growth arrest of K562 cells expressing siRNA against RPS19 was due to the induction of cell cycle arrest at G1 phase (G0/G1 54 % vs 36% ) coincide with the up-regulation of p21 and p57 mRNA level. From these results, we could possibly suggested that, in DBA patients, RPS19 protein expression is not stable and the decreased level of RPS19 protein prolongs G1 phase of cell cycle especially in the course of differentiation on erythroid cells, which may contribute to the retarded production of red blood cells. Our findings allow to understand the entire feature of DBA-associated RPS19 mutations especially on the erythorid differentiation and may help defining the therapeutic target to improve DBA.
- Published
- 2006
69. Identification of Transcripts Commonly Expressed in Both Hematopoietic and Germ-Line Stem Cells.
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Takuo Mizukami, Madoka Kuramitsu, Kazuya Takizawa, Haruka Momose, Atsuko Masumi, Seishiro Naito, Atsushi Iwama, Takehiko Ogawa, Toshiaki Noce, Isao Hamaguchi, and Kazunari Yamaguchi
- Subjects
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GERM cells , *STEM cells , *HISTOLOGY , *GENE expression - Abstract
Germ-line stem cells (GSCs) constitute a stem cell population with remarkable stability and proliferative potential in vitro and are a useful model for studying the mechanism of self-renewal and “stemness” function of committed tissue stem cells. To identify GSC-specific genes, we performed subtractive hybridization using cDNA from GSCs, testis, and embryonic stem (ES) cells, and successfully identified 11 genes highly expressed in GSCs. Histological analysis confirmed expression of Cryαb, Mcpt8, Cxcl5, Fth1, Ctla2α, and Spp1in undifferentiated spermatogonia on the basement membrane area of the seminiferous epithelium of the testis, where the GSC niche is thought to be located. Among GSC-specific genes, quantitative PCR analysis showed seven genes—Fth1, Cryαb, Spp1, Bcap31, Arhgap1, Ctla2α, and Serpina3g—to be common transcripts highly expressed in hematopoietic stem cells (HSCs). Histological analysis confirmed that Ctla2α-, Serpina3g-, and Spp1-expressing cells were observed in the trabecular bone region of the bone marrow, where the HSC niche is located. Furthermore, histological analysis revealed that only Spp1was expressed in the hair follicle bulge in the area of the hair follicle stem cell niche. Thus, identifying stemness genes by comparative analysis to GSCs is a powerful tool with which to explore the fundamental commonalities of HSCs and other stem cell types. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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70. Control of Human T-Cell Leukemia Virus Type 1 (HTLV-1) Infection by Eliminating Envelope Protein-Positive Cells with Recombinant Vesicular Stomatitis Viruses Encoding HTLV-1 Primary Receptor.
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Kenta Tezuk, Kazu Okuma, Madoka Kuramitsu, Sahoko Matsuoka, Reiko Tanaka, Yuetsu Tanaka, and Isao Hamaguchi
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- *
HTLV-I , *ADULT T-cell leukemia , *VIRAL proteins , *STOMATITIS , *NEUROPILINS , *HEPARAN sulfate proteoglycans - Abstract
Human T-cell leukemia virus type 1 (HTLV-1) infection causes adult T-cell leukemia (ATL), which is frequently resistant to currently available therapies and has a very poor prognosis. To prevent the development of ATL among carriers, it is important to control HTLV-1-infected cells in infected individuals. Therefore, the establishment of novel therapies with drugs specifically targeting infected cells is urgently required. This study aimed to develop a potential therapy by generating recombinant vesicular stomatitis viruses (rVSVs) that lack an envelope glycoprotein G and instead encode an HTLV-1 receptor with human glucose transporter 1 (GLUT1), neuropilin 1 (NRP1), or heparan sulfate proteoglycans (HSPGs), including syndecan 1 (SDC1), designated VSVΔG-GL, VSVΔG-NP, or VSVΔG-SD, respectively. In an attempt to enhance the infectivity of rVSV against HTLV-1-infected cells, we also constructed rVSVs with a combination of two or three receptor genes, designated VSVΔGGLN and VSVΔG-GLNS, respectively. The present study demonstrates VSVΔG-GL, VSVΔG-NP, VSVΔG-GLN, and VSVΔG-GLNS have tropism for HTLV-1 envelope (Env)-expressing cells. Notably, the inoculation of VSVΔG-GL or VSVΔG-NP significantly eliminated HTLV-1-infected cells under the culture conditions. Furthermore, in an HTLV-1-infected humanized mouse model, VSVΔG-NP was capable of efficiently preventing HTLV-1-induced leukocytosis in the periphery and eliminating HTLV-1-infected Env-expressing cells in the lymphoid tissues. In summary, an rVSV engineered to express HTLV-1 primary receptor, especially human NRP1, may represent a drug candidate that has potential for the development of unique virotherapy against HTLV-1 de novo infection. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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71. HTLV-1 targets human placental trophoblasts in seropositive pregnant women.
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Kenta Tezuka, Naoki Fuchi, Kazu Okuma, Takashi Tsukiyama, Shoko Miura, Yuri Hasegawa, Ai Nagata, Nahoko Komatsu, Hiroo Hasegawa, Daisuke Sasaki, Eita Sasaki, Takuo Mizukami, Madoka Kuramitsu, Sahoko Matsuoka, Katsunori Yanagihara, Kiyonori Miura, Isao Hamaguchi, Tezuka, Kenta, Fuchi, Naoki, and Okuma, Kazu
- Subjects
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HTLV , *PREGNANT women , *CORD blood , *VIRAL antigens , *RNA virus infections , *BLASTOCYST , *RESEARCH , *TROPHOBLAST , *COMMUNICABLE diseases , *CELL culture , *RETROVIRUSES , *RESEARCH methodology , *EVALUATION research , *COMPARATIVE studies , *PREGNANCY complications , *VERTICAL transmission (Communicable diseases) , *INFECTIOUS disease transmission - Abstract
Human T cell leukemia virus type 1 (HTLV-1) is mainly transmitted vertically through breast milk. The rate of mother-to-child transmission (MTCT) through formula feeding, although significantly lower than through breastfeeding, is approximately 2.4%-3.6%, suggesting the possibility of alternative transmission routes. MTCT of HTLV-1 might occur through the uterus, birth canal, or placental tissues; the latter is known as transplacental transmission. Here, we found that HTLV-1 proviral DNA was present in the placental villous tissues of the fetuses of nearly half of pregnant carriers and in a small number of cord blood samples. An RNA ISH assay showed that HTLV-1-expressing cells were present in nearly all subjects with HTLV-1-positive placental villous tissues, and their frequency was significantly higher in subjects with HTLV-1-positive cord blood samples. Furthermore, placental villous trophoblasts expressed HTLV-1 receptors and showed increased susceptibility to HTLV-1 infection. In addition, HTLV-1-infected trophoblasts expressed high levels of viral antigens and promoted the de novo infection of target T cells in a humanized mouse model. In summary, during pregnancy of HTLV-1 carriers, HTLV-1 was highly expressed in placental villous tissues, and villous trophoblasts showed high HTLV-1 sensitivity, suggesting that MTCT of HTLV-1 occurs through the placenta. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
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