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Raspravljeno je sklanjajne pomoćnih glagoja biti i htjeti.

U povodu 60. obljetnice smrti Ivana Plotnikova, jednoga od utemeljitelja kemijsko-tehnološkoga studija, opisan je njegov život i rad.

Hrvatski inženjerski savez (HIS) organizirao je obilježavanje Prvoga dana inženjera RH obilježavajući 137. obljetnicu utemeljenja Kluba inžinirah i arhitektah u Zagrebu (1878.)

Prikazan je život i djelo hrvatskoga nobelovca Vladimira Preloga koji je na svoj put prema Nobelovoj nagradi(1975.) krenuo iz Zavoda za organsku kemiju današnjega Fakulteta kemijskog inženjerstva i tehnologije. Njegov je životopis uvršten u on-line izdanje Hrvatakog biografkog leksikona

Opisani je život i djelo Štefice Cerjan-Stefanović, redovite profesorice u trajnome zvanju. Dodana je njezina bibliografija.

Nowadays there is a growing concern about the effects of pharmaceuticals in the environment and many reports have been published on the occurrence, fate and risk assessment of veterinary drugs and other pharmaceuticals. Since most pharmaceuticals exhibit great chemical stability and high sorption coefficients in sediments and soils, they can accumulate in these solid environmental compartments and maintain detectable and biologically active concentrations for several months. Compared to water media soils and sediments have been scarcely investigated. Only few reports has been published on the potential contamination caused by these pharmaceuticals residues in sediments samples. To determine the levels at which pharmaceuticals accumulate in different environmental media and to study the cycle of veterinary drugs released in the environment, appropriate analytical methods are necessary. However, residue analysis in these complex samples requires, in addition to a sensitive chromatographic method, an extraction procedure that provides suitable recoveries for the analytes and a clean-up step to remove some of the co-extracted compounds. The objective of this research was to develope and optimizes a simple and effective sample preparation method for determination of selected pharmaceuticals in sediment samples. Pharmaceuticals investigated were: fluoroquinolone antibiotic ciprofloxacin, glucocorticosteroid dexamethasone, anthelmintic febantel, sulfonamide antibiotic sulfamethoxazole and their synergist trimethoprim. Sediments used for experimental work were from Birovača and Kupirovo in Lika, Croatia and had never been treated with any pharmaceuticals. Ultrasonic assisted extraction (UAE) and microwave accelerated extraction (MAE) were optimized to improve efficiency of the extraction process. Extraction procedures were optimized regarding time and temperature of extraction and solvent volume. Extraction efficiency for both extraction procedures, UAE and MAE, was determined by recovery experiments. The extracts were analyzed by high-performance liquid chromatography with diode array detector at 254 nm equipped with a C-18 column (Synergy Fusion 4µ ; m, 150 mm x 2 mm, Phenomenex). The mobile phase used was a mixture of 0.01 % formic acid in water and 0.01 % formic acid in acetonitrile at flow rate 1 mL/min with gradient elution. The injection volume was 30 µ ; L. The optimal conditions determined for UAE were 35 oC, solvent volume 20 mL of citric acid (pH=3.5) : acetonitrile = 20 : 80, (v/v) and extraction time 15 min at 600 W. The optimal conditions determined for MAE were 65 oC, solvent volume 15 mL of citric acid (pH=3.5) : acetonitrile = 20 : 80, (v/v) and extraction time 10 min at 600 W.

After certain pathways and reactions in human or animal body, various metabolites and sometimes unchanged parent compound are excreted through urine or feces. Entering the environment, pharmaceuticals and their metabolites are exposed to sunlight and other conditions which can cause their degradation. The identification of degradation products represents a major challenge to improve understanding of the environmental fate of pharmaceuticals. Analyses were performed on anthelmintics, veterinary drugs that are used to treat parasitic worm infections. Due to their use in great quantities in veterinary practice and agriculture anthelmintics have the potential to appear in surface waters. The main goal in this study was to determine the parent compound and to find out whether it photodegrades and if so, to determine and identify the photodegradation products. Albendazole (ABZ), febantel (FEB) and levamisole hydrochloride (LEV HCl) were analyzed. Samples were exposed to UV light of 254 nm. Even though the UV light of 254 nm could not pervade to the Earth surface, irradiations were carried out at the given wavelength to determine as much photodegradation products as possible. The LC analyses of irradiated water samples were performed by Varian ProStar 500 HPLC system using ProStar 330 diode array detector (Walnut Creek, California, USA) on C18 modified column Synergi Fusion-RP (Phenomenex, USA), 150×2.0 mm, particle size 4 µ m and gradient elution by binary mixture of solvents A (water with 0, 01% formic acid) and B (acetonitrile with 0, 01% formic acid) as mobile phase. The results show that all three analyzed pharmaceuticals photodegrade to one or more products.

One of the main objectives of UKF project REPHAD - Reduction of environmental risks posed by pharmaceuticals and their degradation products in process wastewaters, through RO/NF membrane treatment – is the data collection and pharmaceuticals database creation. Up today many data are collected and organized in REPHAD pharmaceutical database. Data of about 50 pharmaceuticals detected in Croatian water samples are included in the database. Data are organized in four arrays: General information, Physical and chemical properties, Degradation products/metabolites and Determination. General information comprises data such as pharmaceutical name, structural formula, IUPAC name, CAS number, chemical formula and known degradation products/metabolites. Selecting Physical and chemical properties user will find data about pharmaceutical molecular weight, solubility, vapour pressure, stability, log KOW, pKa, half-life and many more information from available literature. Degradation products/metabolites array consists of data of known degradation products and metabolites of pharmaceuticals included in the database: name of degradation products, structural formulas, types of degradation, degradation experiments, analytical methods for degradation products determination. Analytical techniques for pharmaceuticals determination in different water samples (effluent and influent wastewater, production water, municipal wastewater, river water, model wastewater...) are contained in Determination array. Sample pre-treatment, sample preparation and chromatographic conditions are presented. For all data presented in the REPHAD database, literature references with links to manuscripts web page are provided. Pharmaceuticals database will be available on the REPHAD web page (www.fkit.hr/ukf).

Dan je kratak prikaz trorječnika polimerstva autora Igora i Ranke Čatić.

Članak posvećen životu i djelu Vladimira Njegovana u povodu 130 obljetnice njegova rođenja

Razgovorom s akademikom Nenadom Trinajstićem prikazan je njegov život i prinos znanosti.

Dan je popis hrvatskih kemijskih inženjera kojh su životopisi su uvršteni u 8. svezak Hrvatskog bibliografskog leksikona LZ Miroslav Krleža

Enciklopedijski rječnik analitičkoga nazivlja obuhvaća oko 3000 naziva i kratkih definicija iz analitike kao multidisciplinarne kemijske znanosti. Uza natuknice su navedene i engleske istovrijednice te dodatno englesko-hrvatski rječnik.Pokrate i znakovi koji su rabljeni u Rječniku navedeni su na početku knjige.Uza svako slovo dodan je životopis jednoga hrvatskog analitičara, a na kraju Rječnika su kratki podatci o znanstvenicima i izumiteljima koji se spominju u tekstu.

Farmaceutici se svakodnevno upotrebljavaju u velikim količinama te se stoga kontinuirano unose u okoliš. Iako su koncentracije farmaceutika određene u okolišu male, još uvijek se ispituje utjecaj kontinuirane izloženosti i tako malim koncentracijama te smjesama različitih farmaceutika prisutnim u okolišu. Najznačajnije mjesto prikupljanja i ispuštanja farmaceutika u okoliš su postrojenja za obradu otpadnih voda. Ispitivanja ispusnih voda i riječnih sedimenata pokazala su da postojeća postrojenja za obradu voda mogu samo djelomično ukloniti faramceutike i njihove metabolite. Stoga je potrebno razviti postupke obrade voda koji će onemogućiti njihovo ispuštanje u okoliš. U ovom radu praćena je fotolitička i fotokatalitička razgradnja prazikvantela tekućinskom kromatografijom visoke djelotvornosti vezanom sa spektrometrijom masa (HPLC-MS/MS).. Otopine prazikvantela su pripremljene u koncentracijama 1 i 10 mg/L te podvrgnute fotolitičkoj (pri 254 i 365 nm) i fotokatalitičkoj (uz TiO2 film) razgradnji. Praćena je brzina razgradnje prazikvatela te nastajanje i razgradnja razgradnih produkata. U eksperimentima provedenim pri 254 nm prazikvantel se u potpunosti razgradio unutar ispitivanih 90 min. Dodatakom TiO2 kao katalizatora, razgradnja je očekivano ubrzana te je prazikvantel u potpunosti razgrađen u ispitivanom vremenu od 60 min. U eksperimentima fotolitičke i fotokatalitičke razgradnje pri 365 nm nije postignuta potpuna razgradnja prazikvantela. Uz praćenje razgradnje prazikvantela, važan dio ovoga rada je identifikacija nastalih razgradnih produkata. Detektirani su razgradni produkti sljedećih m/z omjera: m/z 273, m/z 269, m/z 189, m/z 147 i m/z 132 te su, na temelju masenih spektara, predložene njihove strukturne formule.

Osvrt na jubilarni koncert klape Nostalgija

Izložena je i raspravljena primjena načela zelene kemije u kemijskoj analizi.

Prikazan je život i rad dr. sc. Biserke Kulušić, znanstvene savjetnice

Many veterinary pharmaceutical industries use the surface water as a recipient for wastewater effluent after treatment. Those wastewaters are usually loaded with very high concentration of pharmaceuticals, especially with veterinary antibiotics. Classical wastewater treatment plants are not suitable for removing pharmaceuticals from wastewaters of pharmaceutical industry, so, they can get into the receiving surface waters with the effluents. From these reasons it is necessary to monitor pharmaceuticals in wastewaters before and after treatment. In this purpose it was developed a robust, simple, realistic and practical analytical method capable of simultaneous determination of target compounds [sulfonamides(sulfaguanidine, sulfadiazine, and sulfadimidine), a sulfonamide synergist (trimethoprim), a tetracycline (oxytetracycline), fluoroquinolones (ciprofloxacin, enrofloksacin, norfloksacin) and local anesthetic procaine], by HPLC with diode array (DAD) and fluorescence (FLD) detectors. A liquid chromatographic method with fluorimetric detection is proposed for the determination of pharmaceuticals in wastewater samples because of highly fluorescent fluoroquinolones (FQ). Limit of detection for FQ is almost ten times lower with HPLC-FLD then with HPLC-DAD. Other investigated pharmaceuticals need to be derivatized with a fluorescamine solution in acetonitrile (5 mg/10 mL acetonitrile) and acetic buffer for determination on HPLC-FLD. The fluorescent compounds are detected at different λex/λem (nm/nm) values: sulfonamides, trimethoprim, oxytetracycline and procaine at 405/495 and fluoroquinolones at 277/445. The analytes are preconcentrated by solid phase extraction (SPE) using Strata-X cartridges and acetonitrile as eluent. The chromatographic separation is performed on a Synergy Fusion-RP18 80 Å, 150 mm × 4.6 mm, particle size 4 microm column with a gradient elution program based on binary mixtures of solvents A (0.1% acetic acid in water) and B (0.1% acetic acid in acetonitrile). The flow rate was 1.0 mL/min. The performance characteristics of the SPE-HPLC-FLD method were established by validation procedure. Selectivity, linearity, limits of detection (LOD) and quantification (LOQ), precision and recovery were studied. The described method is applied to the determination of the investigated pharmaceuticals in pharmaceutical industry wastewater.

Rasprava o izdavanju novoga hrvatskog pravopisa

Vjera Marjanović -Krajovan (189. - 1988.) prva je žena u Hrvatskoj koja je postigla doktorat tehničkih znanosti (1928). Dugogodišnja profesorica analitičke kemije na Kemijsko-tehnološkom fakultetu Sveučilšta u Zagrebu koja je unaprijedila naastavu i bila mentorica mnogih diplomskih, magistarskih radova i disertacija. U knjizi je prikazana biografija prof. Vjere Marjanović-Krajovan.

U povodu 50 broja Glasnika načinjen je pregled zbivanja u AMACIZ-u i na FKIT-u objavljenih u Glasniku od prvoga broja (travanj 1992.) do broja 49 (listopad 2012.)

The presence of pharmaceuticals in the environment has been a great concern among scientists in the last few decades. They have been classified as emerging contaminants and their presence should be investigated in the environment. There are many ways pharmaceuticals reach the environment but the most significant one is via wastewater treatment plants (WWTP), because some of the pharmaceuticals are not eliminated completely during the treatment. Majority of those end up in the water systems and can reach drinking water sources. Once present in the environment they can have negative effects on organisms and influence the equilibrium of ecosystems. Their possible impact on human health also cannot be neglected. This environmental issue is still present and there is a constant need to develop new analytical methods for the determination and quantification of pharmaceuticals in the environment. The aim of this study was to develop a method based on solid phase extraction (SPE) followed by liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) for simultaneous analysis of 22 pharmaceuticals. The choice of analytes covered six different groups of pharmaceuticals: macrolides, fluoroquinolones, tetracyclines, sulfonamides, anthelmintics and anesthetics. After the MS parameters were optimized, the optimal chromatographic conditions were obtained using phenyl column. The result of sample preparation step optimization was sorbent Oasis HLB, 60 mg with acetonitrile as eluent. Environmental samples are very complex and the matrix can affect the efficiency of ionization process in the ESI. In this study different approaches to compensate matrix effects were investigated in order to obtain more accurate results. After the optimization the method was validated and applied to the analysis of real waste water samples.

Stručna biografija Srećka Turine s naglaskom na njegov zamjetan znanstveni prinos razvoju kromatografije. Bibliografski podatci.

Prikazani su program i izvedba Akademskkoga zbora Vladimir Prelog na koncertu održanom 16. ožujka 2013. u Hrvatskom glazbenom zavodu.

Martin Kranjčević, životopis i djela

Smatra se da esteraze paraoksonaza 1 (PON1), putem sprečavanja oksidacije lipoproteina plazme i butirilkolinesteraza (BuChE), svojim sudjelovanjem u metabolizmu kolina i lipoproteina male gustoće, utječu na metabolizam lipida. Pretpostavlja se da i leptin također sudjeluje u metabolizmu lipida. Osim dobro poznatih učinaka antilipidnih lijekova na lipide, sve važniji postaju njihovi ne-lipidni (pleiotropni) učinci. Stoga su ciljevi ovog rada bili ispitati i usporediti djelovanje višekratne primjene atorvastatina, pravastatina, simvastatina i gemfibrozila na esteraze, lipide, malondialdehid i leptin. Sva istraživanja su provedena na normolipidemičkim Wistar štakorima. Atorvastatin i pravastatin su izazvali porast, a simvastatin pad katalitičke aktivnosti serumske i jetrene PON1. Promjene u koncentracijama lipida plazme bile su karakteristične kako za same primjenjene lijekove tako i za korištenu životinjsku vrstu. Svi statini su smanjili koncentraciju malondialdehida (antioksidacijski učinak), osobito simvastatin. Suprotno je bilo u životinja tretiranih gemfibrozilom, tj. rezultati su jasno pokazali da isti lijek smanjuje aktivnost PON1 u jetri i povećava koncentraciju malondialdehida (oksidacijski učinak). Statini su povećali aktivnost BuChE u plazmi i jetri, a gemfibrozil u plazmi. Smanjenje plazmatske koncentracije leptina je izmjereno nakon primjene gemfibrozila i manjih doza statina. Prema ovim rezultatima, statini pokazuju bolje pleiotropne učinke od gemfibrozila, naročito kada je u pitanju njihov antioksidacijski učinak.

Prikaz koncerta održanoga u Hrvatskom glazbenom zavodu u ožujku 2012.

Uvodni članak novopokrenute rubrike Imenje i nazivlje u kemiji i kemijskom inženjertsvu u kojemu se obrazlaže potreba razvijanja hrvatskoga strukovnoga nazivlja i poziva na suradnju.

Poticaj za ponovno pokretanje Zaklade Fakulteta kemijskog inženjerstva i tehnologije koja je 1991-2012. stipendirala i nagrađivala brojne izvrsne studente, među kojima je i desetak današnjih nastavnika FKIT-a.

Rastumačene su preporuke sklanjanja imenica koje završavaju na -ak i -ac te način pisanja izgovora pokrata engleskih strukovnih naziva.

Prikazana je povijest kemije u Republici Hrvatskoj u zadnjem desetljeæu XX. stoljeća. To je desetljeće obilježila borba za neovisnošću, koju je karakterizirala do 1995. obrana domovine oružjem, a od 1995. do kraja stoljeća izgradnja institucija neovisne drÞave. Naravno da znanstvenici nisu mogli ostati po strani, pa su neki uzeli oružje u ruke, a drugi širili svijetom istinu o Domovinskome ratu. Razmatrana je djelatnost kemičara na Prirodoslovno-matematičkome fakultetu, Institutu Rugjer Bošković i ostalim fakultetima na Sveučilištu u Zagrebu. Prikazana je transformacija kemijsko-tehnološkoga studija u Fakultet kemijskoga inÞenjerstva i tehnologije.

Savjeti o ispravnoj uporabi hrvatskih naziva u struci i znanosti.

Smatra se da esteraze paraoksonaza 1 (PON1), putem sprečavanja oksidacije lipoproteina plazme i butirilkolinesteraza (BuChE), svojim sudjelovanjem u metabolizmu kolina i lipoproteina male gustoće, utječu na metabolizam lipida. Pretpostavlja se da i leptin također sudjeluje u metabolizmu lipida. Osim dobro poznatih učinaka antilipidnih lijekova na lipide, sve važniji postaju njihovi ne-lipidni (pleiotropni) učinci. Stoga su ciljevi ovog rada bili ispitati i usporediti djelovanje višekratne primjene atorvastatina, pravastatina, simvastatina i gemfibrozila na esteraze, lipide, malondialdehid i leptin. Sva istraživanja su provedena na normolipidemičkim Wistar štakorima. Atorvastatin i pravastatin su izazvali porast, a simvastatin pad katalitičke aktivnosti serumske i jetrene PON1. Promjene u koncentracijama lipida plazme bile su karakteristične kako za same primjenjene lijekove tako i za korištenu životinjsku vrstu. Svi statini su smanjili koncentraciju malondialdehida (antioksidacijski učinak), osobito simvastatin. Suprotno je bilo u životinja tretiranih gemfibrozilom, tj. rezultati su jasno pokazali da isti lijek smanjuje aktivnost PON1 u jetri i povećava koncentraciju malondialdehida (oksidacijski učinak). Statini su povećali aktivnost BuChE u plazmi i jetri, a gemfibrozil u plazmi. Smanjenje plazmatske koncentracije leptina je izmjereno nakon primjene gemfibrozila i manjih doza statina. Prema ovim rezultatima, statini pokazuju bolje pleiotropne učinke od gemfibrozila, naročito kada je u pitanju njihov antioksidacijski učinak., It is believed that paraoxonase 1 (PON1) and butyrylcholinesterase (BuChE) are important in the lipid metabolism. PON1 is supposed to be able to prevent the oxidation of plasma lipoproteins. BuChE might be included in the metabolism of choline and LDL. It seems that leptin is also involved in the lipid metabolism. Beside well-known lipid effects expressed by antilipid drugs, some studies indicate their other important non-lipid (pleiotropic) effects. Therefore the aims of this study were to investigate the influence of multiple applications of atorvastatin, pravastatin, simvastatin and gemfibrozil on esterases, lipids, malondyaldehide and leptin. All studies were performed on normolipidemic Wistar rats. Atorvastatin and pravastatin increased, and simvastatin decreased PON1 activity in serum and liver. All changes on plasma lipids were typical for the used drugs and treated animals. All of the statins decreased the malondyaldehide concentration (antioxidative effect), simvastatin in particular. The opposite effects were found in animals treated by gemfibrozil, i.e. the decrease of PON1 activity in liver and increase of malondyaldehide concentration (oxidative effect). Statins caused the increase of BuChE activity in plasma and liver, while gemfibrozil raised its activity in plasma. The low concentration of plasma leptin was measured after the administration of gemfibrozil and also of low doses of statins. According to these results, when compared with gemfibrozil, statins show preferable pleiotropic, especially antioxidative effects.

There are diverse experimental data about the influence of gemfibrozil (GEM) on the production of hydrogen peroxide (H(2)O(2)) and antioxidant enzymes. We investigated the influence of GEM treatment on the production of malondialdehyde (MDA) level in tissues of normolipidaemic Wistar and Fisher rats which is an index of lipid peroxidation. Because serum paraoxonase 1 (PON1) is an important enzyme with specific protective function on metabolism of lipid peroxides, we examined the influence of GEM on PON1 activity in liver and serum. MDA level and enzyme activities were also determined 10 days after withdrawal of GEM treatment. The significantly increased levels of MDA in liver, kidney and heart of both rat strains were obtained after 3 weeks of GEM treatment. We propose two possibilities for the increase of MDA levels caused by GEM, induction of peroxisome proliferation and activities of enzymes that participated in occurrence of H(2)O(2) and possible reduction of enzyme activities including in H(2)O(2) metabolism. Ten days after withdrawal of GEM treatment, MDA levels in all tissue levels of both rat strains were less in comparison with GEM treatment. GEM caused a significant drop of PON1 activity in serum and liver of Fisher rats, and in liver of Wistar rats. We suggest that GEM, through induction of lipid peroxidation, caused the damage of hepatocytes with consequent reduction of PON1 synthesis. The increase in PON1 activity in serum and tissues of both rat strains 10 days after withdrawal of GEM treatment shows the fast recovery of enzyme synthesis.

Prikazana je knjižica Akademski zbor Vladimir Prelog, 20 godina djelovanja 1991.-2011. urednika Krune Kovačevića i DVD Zov glazbe redatelja Tihonija Brčića i autorice sinopsisa Marije Kaštelan-Macan.

Pharmaceuticals, both in their parent form and as metabolites, are commonly released into the environment at trace levels through conventional sewage treatment plants. Therefore, it is not surprising that research has recently been directed toward the application of non-biological processes for the destruction of pharmaceuticals in water system with the emphasis on advanced oxidation processes (AOPs). If the mineralization is not complete, the system may contain more toxic organic substances. So, the evaluation of toxicity by measuring the bioluminescence inhibition of Vibrio fischeri bioassay after AOPs (Fenton process) was performed on investigated veterinary pharmaceuticals: fluoroquinolone antibiotic ciprofloxacin, glucocorticosteroid dexamethasone, anthelmintic febantel, sulfonamide antibiotic sulfamethoxazole an their synergist trimethoprim, which have a wide production and use in Croatia and abroad. The results indicate that all investigated pharmaceuticals can cause significant adverse effect on the tested bacteria Vibrio fischeri by prolonged and continuous input of investigated pharmaceuticals in the environment.

Obrađene su teme sklanjanja pridjeva, pisanja velikih i malih slova te navedene neki izrazi neprikladni hrvatskome jeziku.

U predavanju je prikazan utjecaj na razvoj tankoslojne kromatografije u Zavodu za analitičku kemijuFKIT-a i velika uloga dr. sci. Srećka Turine u odgoju mladiuh kromatografičara, što je rezultiralo činjenicom da je Zavod danas jedan od vodećih kromatografskih centara u Hrvatskoj

Prikazan je sveučilišni udžbenik Ivana Piljca koji može polsužiti i kao izvrstan priručnik i izvor novih spoznaja iz toga područja.

Knjiga Senzori fizikalnih veličina i elektroanalitičke metode autora Ivana Piljca nema uzora u hrvatskoj znanstveno-stručnoj literaturi. Opširna građa na izvoran je način obrađena u četrnaest poglavlja, od kojih svako čini zasebnu cjelinu, pa studentima prirodoslovnih, tehničkih i srodnih fakulteta omogućuje izbor poglavlja u skladu s nastavnim programom studija koji pohađaju. Izvrstan je priručnik i njihovim nastavnicima te stručnjacima u praksi.

Opisana su zbivanja i nastojanja da se Fakultet kemijskoga inženjerstva i tehnologije osamostali, dobije pravo organiziranja doktorskih studija i izbora u zvanja u poljima kemija i kemijsko inženjerstvo. Fakultet je započeo samostalnim radom 16. studenoga 1991.

Opisano je svečano obilježavanje 50 godina tekstilnoga studija i 20. obljetnice Tekstilno-tehnološkoga fakulteta Sveučilišta u Zagrebu. Tom su prigodom uz zanimljiv program predstavljena Monografija TTF-a i dodijeljene plakete zaslužnim profesorima.

Alloys are designed to optimize certain technical properties, such as mechanical strength, workability, wear and corrosion. If the metal objects involved are unique in nature (e.g., statues, utility objects of different shapes and sizes in alloy), it is often necessary to sample with minor or even without destruction. There are only few methods of chemical analysis known to satisfy these conditions. External beam proton induced X-ray emission spectrometry (PIXE) and X-ray fluorescent (XRF) analysis are nondestructive methods for in situ measurements, but they require sophisticated and expensive equipment. [1] The advantage of nondestructive methods is the possibility to measure a larger number of surface samples taken at various positions on the object. In this work anodic sampling hyphenated with thin-layer chromatography was used with videoimaging system for archiving and processing of chromatograms. Hence, the influence of instrumental settings of a video-imaging system on quality of captured images has been studied. Performances of the CAMAG Video Documentation System were evaluated by the measurements of absorption quenching for model standard solutions of metal ions of copper, tin and nickel in visible (VIS) spectral region. The effects of different camera settings (aperture and number of accumulated frames) on the background response, baseline noise and reproducibility of detection were studied on commercial cellulose TLC plates. [2] The results showed that as the number of frames, the image brightness and background response increased, the sensitivity increased too. Plates captured and illuminated from up and both modes gave more repeatable results. Repeatability of determination (RSD) was ranged from 9, 2-32, 3% for copper ions, 10, 9-52, 2% for tin ions and 8, 8-56, 4% for nickel ions.

Pharmaceuticals cover a wide range of different compounds and are used to cure disease and fight infection both in humans and in animals. Their occurrence in the environment has become an issue of great importance in the last decade. Because of their wide consumption they have been used in large quantities for years and for the last few decades they have been investigated as a new type of so called emerging contaminants. In the environment they can be found in low concentrations of μg/L or ng/L but their continuous input can pose a potential long term risk for aquatic and terrestrial organisms as well as for human health. [1] To detect and quantify pharmaceuticals in the environment is very complicated because the matrices in which they occur are complex, they have very diverse physicochemical properties and they are found in very low concentrations. Therefore it is of great importance to develop analytical methods that will be highly sensitive and selective in order to monitor pharmaceuticals in the environment. [2] Liquid chromatography coupled to mass spectrometry especially multidimensional mass spectrometry (LC-MSn) is a powerful technique which can provide adequate specificity and sensitivity. [3] The aim of this work was to develop a SPE-HPLC-ESI(+)-MS/MS analytical method for determination pharmaceuticals in wastewater. Investigated pharmaceuticals belong to different classes of antimicrobials: sulfonamides (sulfaguanidine, sulfadiazine, sulfamethazine and sulfametoxazole), a sulfonamide synergist from diaminopyrimidine class (trimethoprim), fluoroquinolones (ciprofloxacin, enrofloxacin and norfloxacin), macrolides (azithromycin, erythromycin, clarithromycin, roxythromycin, tylosin, azahomoerithromycin), tetracyclines (oxytetracycline, tetracycline), antihelmintics (febantel, levamisol, praziquantel), anestetics (lidocain, procain), a steroid hormone (dexametazone) and antibiotic (tiamulin). Sample preparation was done by solid phase extraction (SPE) and the pharmaceuticals were extracted from water using optimized SPE system. Chromatographic separation was done on HPLC – MS/MS Agilent 1200 and the detection on Agilent 6410 triple quadrupole mass spectrometer equipped with electrospray ionization (ESI). Mobile phase was 0.1% formic acid in water and 0.1% formic acid in acetonitrile in gradient mode and the chromatographic column was phenyl Synergi Polar 2, 5μm, 100 x 2.0 mm (Phenomenex)

Opisano je plodno dugogodišnje djelovanje akademika Velimira Pravdića na razvoju kemijeke znanosti i promicanju održiva razvitka u Hrvatskoj u svijetu

U predavanju je u povodu smrti Srećka Turine, vizionara razvoja kromatografije u Hrvatskoj priokazan njegov život i postignuća unatoč neprikladnim uvjetima.

U predavanju za predstavnike hrvatskih laboratorija predloženi hrvatski nazivi metoda, postupaka i laboratproijske opreme koji bi trebali smanjiti uporabu uobuičajenih engleskih naziva.

Opisam je sadržaj knjige Analitika okoliša koja, nakon uvodnoga poglavlja o kemiji okoliša, govori o vrstama, izvorima i putovanju zagađivala u okolišu, kemijskim i biokemijskim metodama njihova određivanja te kemometričkim postupcima i osiguravanju kvalitete kemijskoga mjerenja.

Hrvatsko-engleski rječnik oko 1700 strukovnih pojmova iz analitike s definicijama pojmova. Rukopis u fazi sređivanja za tisak