63 results on '"Loosen, S."'
Search Results
52. Prevalence of Hospitalization and Readmission in Patients with Inflammatory Bowel Diseases.
- Author
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Kostev K, Loosen S, and Roderburg C
- Subjects
- Hospitalization, Humans, Prevalence, Retrospective Studies, Risk Factors, Inflammatory Bowel Diseases epidemiology, Inflammatory Bowel Diseases therapy, Patient Readmission
- Published
- 2022
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53. An elevated FIB-4 score is not associated with cardiovascular events: a longitudinal analysis from 137 842 patients with and without chronic liver disease.
- Author
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Loosen S, Luedde M, Demir M, Luedde T, Kostev K, and Roderburg C
- Subjects
- Humans, Liver Cirrhosis complications, Liver Cirrhosis diagnosis, Liver Cirrhosis epidemiology, Ischemic Attack, Transient complications, Ischemic Attack, Transient diagnosis, Ischemic Attack, Transient epidemiology, Myocardial Infarction diagnosis, Myocardial Infarction epidemiology, Non-alcoholic Fatty Liver Disease epidemiology, Stroke complications, Stroke diagnosis, Stroke epidemiology
- Abstract
Background: Liver diseases and cardiovascular diseases are closely associated. Noninvasive tests (NITs), such as the FIB-4 score, have been recommended by different guidelines to rule out advanced fibrosis and estimate the risk of liver-related outcomes in patients with chronic liver diseases (CLDs). However, the association of an elevated FIB-4 score with the development of myocardial infarction and/or stroke/transient ischemic attack (TIA) is unknown., Methods: By using the Disease Analyzer database (IQVIA), which compiles diagnoses, laboratory values as well as basic medical and demographic data of over 7.5 million patients followed in general practices in Germany, we identified 68 921 patients with available lab values for FIB-4 score calculation between 2005 and 2019. Patients with an FIB-4 score of less than 1.3 were matched 1:1 to patients with an FIB-4 score of at least 1.3 by age, sex and yearly consultation frequency., Results: In regression analysis, the incidence rate ratio (IRR) of myocardial infarction was similar among patients with an FIB-4 score of at least 1.3 compared with patients with a low FIB-4 score of less than 1.3, regardless of whether patients with or without preexisting liver disease were analyzed. Moreover, there was no significant increase in the IRR of stroke/TIA between patients with a high or low FIB-4 score, both in the general population and patients with CLD., Conclusion: Our study suggests that the FIB-4 score is not associated with cardiovascular events both in the general population as well as in patients with CLD., (Copyright © 2022 Wolters Kluwer Health, Inc. All rights reserved.)
- Published
- 2022
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54. Nonalcoholic fatty liver disease is associated with a higher incidence of coeliac disease.
- Author
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Roderburg C, Loosen S, Kostev K, Demir M, Joerdens MS, and Luedde T
- Subjects
- Cohort Studies, Humans, Incidence, Male, Risk Factors, Celiac Disease complications, Celiac Disease diagnosis, Celiac Disease epidemiology, Non-alcoholic Fatty Liver Disease complications, Non-alcoholic Fatty Liver Disease diagnosis, Non-alcoholic Fatty Liver Disease epidemiology
- Abstract
Background: Recently the incidence of nonalcoholic fatty liver disease (NAFLD) has risen to become the most frequent liver disease worldwide. NAFLD is not a disease limited to the liver, but rather represents a systemic inflammatory disease involving multiple organ systems. Previous studies have suggested an association between NAFLD and coeliac disease, another disease related to inflammation. As the available clinical data is scarce and at least partially contradictory, we aimed at investigating a potential association between NAFLD and celiac disease in outpatients in Germany., Methods: Using the Disease Analyzer database featuring data on diagnoses, prescriptions and demographic variables for 7.49 million cases of patients followed in general practices in Germany, we matched a total of 57 336 patients with NAFLD/diagnosed between 2000 and 2015 to a cohort of equal size without NAFLD by age, sex and index year. Incidence of celiac disease was compared between groups within 10 years from the index date., Results: During the 10-years observation period, we observed a higher incidence of coeliac disease in NAFLD patients compared to patients without NAFLD (11.2 vs. 7.5 cases per 100 000 patients' years). Interestingly, this association was most prominent in men (hazard ratio, 2.02; 95% CI, 1.01-4.04) and patients between 18 and 50 years (hazard ratio, 2.79; 95% CI, 1.12-6.94)., Summary: Our data suggest that NAFLD promotes the development of coeliac disease particularly in young men. This finding argues that coeliac disease should be recognized as another nonliver-related complication of NAFLD and suggests screening of selected NAFLD patients for the presence of coeliac disease., (Copyright © 2021 Wolters Kluwer Health, Inc. All rights reserved.)
- Published
- 2022
- Full Text
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55. Increased pharmacy purchases of cardiovascular drugs from wholesalers prior to the first and second COVID-19 lockdowns.
- Author
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Luedde M, Loosen S, Konrad M, Tanislav C, Roderburg C, and Kostev K
- Subjects
- Communicable Disease Control, Cross-Sectional Studies, Humans, Retrospective Studies, SARS-CoV-2, COVID-19, Cardiovascular Agents therapeutic use, Pharmacies, Pharmacy
- Abstract
Aim: The aim of this study was to examine the development of drug purchases over the course of the coronavirus crisis in Germany in 2020., Materials and Methods: The evaluations in this retrospective cross-sectional study are based on the IMS RPM (Regional Pharmaceutical Market) weekly database, which shows weekly purchases by public pharmacies from full-service wholesalers at the time the pharmacy purchase is made in Germany. The outcome of this investigation was the development of cardiovascular drug sales by packing unit over all 52 weeks of 2020., Results: We found an increase of 68% in week 12 compared to the average sales for weeks 2 - 11, 2020 (vs. -2% in week 12, 2019), while the increase in week 51 was 61%, compared to the average sales for weeks 13 - 50, 2020 (vs. 35% in week 51, 2019). The largest increases in week 12 were for calcium channel blockers (64%), and the largest increases in week 51 were for lipid-lowering drugs (67%)., Conclusion: The results of this retrospective cross-sectional study suggest that the COVID-19 lockdown in Germany was associated with a significant surge in pharmacy purchases of cardiovascular drugs, indicating panic buying. Although there were no drug shortages during the first lockdown, this panic buying recurred shortly before the second lockdown, albeit to a lesser extent.
- Published
- 2021
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56. [Perception of the COVID-19 Pandemic among Pneumology Professionals in Germany].
- Author
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Peine A, Paffenholz P, Hellmich M, Martin L, Roderburg C, Heidenreich A, Marx G, Trautwein C, Luedde T, and Loosen SH
- Subjects
- Female, Germany epidemiology, Humans, Pandemics prevention & control, Perception, SARS-CoV-2, Surveys and Questionnaires, COVID-19, Pulmonary Medicine
- Abstract
The COVID-19 pandemic represents a huge burden on global health systems. Although far-reaching prevention measures such as the increase of intensive care capacities and drastic restrictions of public life have so far been able to avert an overload of the German health care system, the current situation implies an exceptionally high burden on medical professionals. The current study presents the results of an opinion evaluation among 513 pneumology specialists in Germany in the period from March 27
th to April 11th , 2020. While the majority of respondents stated that Germany was "well" prepared for the pandemic, this assessment was significantly worse among participants from the outpatient sector compared to the hospital sector (p < 0.001). Furthermore, a lack of medical protective equipment was reported significantly more frequently by respondents from the outpatient sector (p < 0.001). The importance of telemedicine approaches during the COVID-19 pandemic was rated "high" (35.2 %) or "very high" (17.2 %) by most pneumology professionals, with participants from the hospital sector giving a higher rating (p < 0.001). Finally, 45.8 % of the respondents expressed a "negative" influence of the COVID-19 pandemic on their personal mood and 58.3 % expressed "strong" or "very strong" concerns about the health of their fellow human beings. This assessment was significantly stronger among female participants and participants from the nursing sector (p < 0.001). In summary, the current study analyses for the first time the professional and personal impact of the COVID-19 pandemic on pneumology professionals in Germany. The results could help to identify first starting points to better support health professionals during the current and future challenges., Competing Interests: Die Autorinnen/Autoren geben an, dass kein Interessenkonflikt besteht. A. P. ist Geschäftsführer und Gesellschafter der Clinomic GmbH. L. M. und G. M. sind Gesellschafter der Clinomic GmbH., (Thieme. All rights reserved.)- Published
- 2021
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57. Serum levels of bone sialoprotein correlate with portal pressure in patients with liver cirrhosis.
- Author
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Benz F, Bogen A, Praktiknjo M, Jansen C, Meyer C, Wree A, Demir M, Loosen S, Vucur M, Schierwagen R, Tacke F, Trebicka J, and Roderburg C
- Subjects
- Adult, Aged, Biomarkers blood, Disease Progression, Female, Humans, Hypertension, Portal blood, Hypertension, Portal physiopathology, Liver Cirrhosis blood, Liver Cirrhosis diagnosis, Liver Cirrhosis physiopathology, Male, Middle Aged, Portal Vein physiopathology, Prognosis, Retrospective Studies, Severity of Illness Index, Hypertension, Portal diagnosis, Integrin-Binding Sialoprotein blood, Liver Cirrhosis complications, Portal Pressure physiology
- Abstract
Liver cirrhosis represents the common end-stage of chronic liver diseases regardless of its etiology. Patients with compensated disease are mostly asymptomatic, however, progression to a decompensated disease stage is common. The available stratification strategies are often unsuitable to identify patients with a higher risk for disease progression and a limited prognosis. SIBLINGs, soluble glycophosphoproteins, are secreted into the blood by immune-cells. While osteopontin, the most prominent member of the SIBLINGs family, has been repeatedly associated with liver cirrhosis, data on the diagnostic and/or prognostic value of bone sialoprotein (BSP) are scarce and partly inconclusive. In this study, we analyzed the diagnostic and prognostic potential of circulating BSP in comparison to other standard laboratory markers in a large cohort of patients with liver cirrhosis receiving transjugular intrahepatic portosystemic shunt (TIPS). Serum levels of BSP were similar in patients with different disease stages and were not indicative for prognosis. Interestingly, BSP serum levels did correlate inversely with portal pressure, as well as its surrogates such as platelet count, the portal vein cross-sectional area and correlated positively with the portal venous velocity. In summary, our data highlight that BSP might represent a previously unrecognized marker for portal hypertension in patients with liver cirrhosis., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2020
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58. miR-155 Predicts Long-Term Mortality in Critically Ill Patients Younger than 65 Years.
- Author
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Tacke F, Spehlmann ME, Vucur M, Benz F, Luedde M, Cardenas DV, Roy S, Loosen S, Hippe HJ, Frey N, Trautwein C, Koch A, Roderburg C, and Luedde T
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Biomarkers blood, Female, Humans, Intensive Care Units, Male, Middle Aged, Prognosis, Real-Time Polymerase Chain Reaction, Young Adult, Critical Illness mortality, MicroRNAs blood, MicroRNAs genetics
- Abstract
Introduction: Alterations in miR-155 serum levels have been described in inflammatory and infectious diseases. Moreover, a role for miR-155 in aging and age-related diseases was recently suggested. We therefore analyzed a potential age-dependent prognostic value of circulating miR-155 as a serum-based marker in critical illness., Methods: Concentrations of circulating miR-155 were determined in 218 critically ill patients and 76 healthy controls., Results: By using qPCR, we demonstrate that miR-155 serum levels are elevated in patients with critical illness when compared to controls. Notably, levels of circulating miR-155 were independent on the severity of disease, the disease etiology, or the presence of sepsis. In the total cohort, miR-155 was not an indicator for patient survival. Intriguingly, when patients were subdivided according to their age upon admission to the ICU into those younger than 65 years, lower levels of miR-155 turned out as a strong marker, indicating patient mortality with a similar accuracy than other markers frequently used to evaluate critically ill patients on a medical ICU., Conclusion: In summary, the data provided within this study suggest an age-specific role of miR-155 as a prognostic biomarker in patients younger than 65 years. Our study is the first to describe an age-dependent miRNA-based prognostic biomarker in human diseases.
- Published
- 2019
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59. microRNA 193a-5p Regulates Levels of Nucleolar- and Spindle-Associated Protein 1 to Suppress Hepatocarcinogenesis.
- Author
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Roy S, Hooiveld GJ, Seehawer M, Caruso S, Heinzmann F, Schneider AT, Frank AK, Cardenas DV, Sonntag R, Luedde M, Trautwein C, Stein I, Pikarsky E, Loosen S, Tacke F, Ringelhan M, Avsaroglu SK, Goga A, Buendia MA, Vucur M, Heikenwalder M, Zucman-Rossi J, Zender L, Roderburg C, and Luedde T
- Subjects
- Animals, Carcinoma, Hepatocellular metabolism, Carcinoma, Hepatocellular prevention & control, Cell Line, Tumor, Cell Proliferation, Gene Expression Regulation, Neoplastic, Humans, Liver metabolism, Liver Neoplasms metabolism, Male, Mice, Mice, Inbred C57BL, Mice, Nude, Microtubule-Associated Proteins metabolism, RNA, Messenger metabolism, RNA, Small Interfering metabolism, Signal Transduction, Xenograft Model Antitumor Assays, Apoptosis genetics, Carcinogenesis genetics, Cell Cycle Proteins metabolism, Liver Neoplasms prevention & control, MicroRNAs metabolism, Nuclear Proteins metabolism
- Abstract
Background & Aims: We performed an integrated analysis to identify microRNAs (miRNAs) and messenger RNAs (mRNAs) with altered expression in liver tumors from 3 mouse models of hepatocellular carcinoma (HCC) and human tumor tissues., Methods: We analyzed miRNA and mRNA expression profiles of liver tissues from mice with diethylnitrosamine-induced hepatocarcinogenesis, conditional expression of lymphotoxin alpha and lymphotoxin beta, or inducible expression of a Myc transgene (Tet-O-Myc mice), as well as male C57BL/6 mice (controls). miRNA mimics were expressed and miRNAs and mRNAs were knocked down in human (Huh7, Hep3B, JHH2) hepatoma cell lines; cells were analyzed for viability, proliferation, apoptosis, migration, and invasion. Cells were grown as xenograft tumors in nude mice and analyzed. We combined in silico target gene prediction with mRNA profiles from all 3 mouse models. We quantified miRNA levels in 146 fresh-frozen tissues from patients (125 HCCs, 17 matched nontumor tissues, and 4 liver samples from patients without cancer) and published human data sets and tested correlations with patient survival times using Kaplan-Meier curves and the log-rank test. Levels of NUSAP1 mRNA were quantified in 237 HCCs and 5 nontumor liver samples using the TaqMan assay., Results: Levels of the miRNA 193a-5p (MIR193A-5p) were reduced in liver tumors from all 3 mouse tumor models and in human HCC samples, compared with nontumor liver tissues. Expression of a MIR193A-5p mimic in hepatoma cells reduced proliferation, survival, migration, and invasion and their growth as xenograft tumors in nude mice. We found nucleolar and spindle-associated protein 1 (NUSAP1) to be a target of MIR193A-5p; HCC cells and tissues with low levels of MIR193A-5p had increased expression of NUSAP1. Increased levels of NUSAP1 in HCC samples correlated with shorter survival times of patients. Knockdown of NUSAP1 in Huh7 cells reduced proliferation, survival, migration, and growth as xenograft tumors in nude mice. Hydrodynamic tail-vein injections of a small hairpin RNA against NUSAP1 reduced growth of Akt1-Myc-induced tumors in mice., Conclusions: MIR193A-5p appears to prevent liver tumorigenesis by reducing levels of NUSAP1. Levels of MIR193A-5p are reduced in mouse and human HCC cells and tissues, leading to increased levels of NUSAP1, associated with shorter survival times of patients. Integrated analyses of miRNAs and mRNAs in tumors from mouse models can lead to identification of therapeutic targets in humans. The currently reported miRNA and mRNA profiling data have been submitted to the Gene Expression Omnibus (super-series accession number GSE102418)., (Copyright © 2018 AGA Institute. Published by Elsevier Inc. All rights reserved.)
- Published
- 2018
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60. The multikinase inhibitor regorafenib decreases angiogenesis and improves portal hypertension.
- Author
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Uschner FE, Schueller F, Nikolova I, Klein S, Schierwagen R, Magdaleno F, Gröschl S, Loosen S, Ritz T, Roderburg C, Vucur M, Kristiansen G, Lammers T, Luedde T, and Trebicka J
- Abstract
Background and Aims: Angiogenesis is critically involved in the development of liver fibrosis, portal hypertension (PHT) and hepatocellular carcinoma (HCC). Regorafenib is a novel second-line therapy for HCC, but might also be beneficial in fibrosis and PHT even in absence of HCC. This study investigated the effects of regorafenib in experimental models without HCC., Methods: Fibrosis ( in vivo and in vitro ), inflammation, liver damage (aminotransferases), angiogenesis (matrigel implantation) and in vivo systemic and portal hemodynamics were assessed in different mouse and rat models (bile duct ligation, CCl
4 , partial portal vein ligation) after acute and chronic treatment with regorafenib., Results: Long-term treatment with regorafenib improved portal hypertension most likely due to blunted angiogenesis, without affecting fibrosis progression or regression. Interestingly, acute administration of regorafenib also ameliorated portal hemodynamics. Although regorafenib treatment led to hepatotoxic side effects in long-term treated fibrotic animals, in partial portal vein ligated rats, no liver toxicity due to regorafenib was observed., Discussion: Regorafenib might be especially suitable as therapy in patients with PHT and preserved liver function., Competing Interests: CONFLICTS OF INTEREST The authors have no conflicts of interest.- Published
- 2018
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61. Elevated serum levels of bone sialoprotein during ICU treatment predict long-term mortality in critically ill patients.
- Author
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Luedde M, Roy S, Hippe HJ, Cardenas DV, Spehlmann M, Vucur M, Hoening P, Loosen S, Frey N, Trautwein C, Luedde T, Koch A, Tacke F, and Roderburg C
- Subjects
- APACHE, Adolescent, Adult, Aged, Aged, 80 and over, Enzyme-Linked Immunosorbent Assay, Female, Humans, Intensive Care Units statistics & numerical data, Male, Middle Aged, Prognosis, Young Adult, Biomarkers blood, Critical Illness mortality, Integrin-Binding Sialoprotein blood
- Abstract
Bone sialoprotein (BSP), a member of the SIBLINGs (for Small Integrin-Binding LIgand, N-linked Glycoproteins) family, has recently be associated to inflammatory and infectious diseases. We therefore measured BSP concentrations in 136 patients at admission to the intensive care unit (ICU) and 3 days of ICU. BSP levels were compared to 36 healthy blood donors and correlated to clinical data. In these analysis, BSP serum levels were strongly elevated at the time point of admission to the ICU when compared to healthy controls. Moreover BSP concentrations were significantly elevated after 3 days of treatment on the intensive care unit. A further increase in BSP levels was detected in patients with higher APACHE-II-scores and in patients with septic disease. While in most patients, BSP levels decreased during the first three days of treatment on a medical ICU, patients with persistently elevated BSP levels displayed an unfavorable outcome. In these patients, persistently elevated BSP concentrations were a superior predictor of mortality than established indicators of patient´ prognosis such as the SAPS2 or the APACHE-II score. In summary, our data argue for a novel utility for BSP as a biomarker in patients treated on a medical ICU.
- Published
- 2018
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62. Down-regulation of miR-192-5p protects from oxidative stress-induced acute liver injury.
- Author
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Roy S, Benz F, Alder J, Bantel H, Janssen J, Vucur M, Gautheron J, Schneider A, Schüller F, Loosen S, Luedde M, Koch A, Tacke F, Luedde T, Trautwein C, and Roderburg C
- Subjects
- Alanine Transaminase blood, Animals, Aspartate Aminotransferases blood, Cells, Cultured, Down-Regulation, Homeodomain Proteins physiology, Humans, Male, Mice, Mice, Inbred C57BL, MicroRNAs blood, Repressor Proteins physiology, Zinc Finger E-box Binding Homeobox 2, Chemical and Drug Induced Liver Injury prevention & control, MicroRNAs physiology, Oxidative Stress
- Abstract
miR-192-5p has gained increasing relevance in various diseases, however, its function in acute liver injury is currently unknown. We analysed miR-192-5p serum levels and hepatic miR-192-5p expression in mice after hepatic ischaemia and reperfusion (I/R) as well as in toxic liver injury. On a functional level, miRNA levels were analysed in the different hepatic cell-compartments and in the context of tumour necrosis factor (TNF)-dependent liver cell death. We detected increased serum levels of miR-192-5p after hepatic I/R- and carbon tetrachloride (CCl4)-induced liver injury. miR-192-5p levels correlated with the degree of liver damage and the presence of hepatic cell death detected by TUNEL stainings (terminal deoxynucleotidyltransferase-mediated dUTP biotin nick-end labelling stainings). Moreover, expression of miR-192-5p was increased in a hypoxia/reoxygenation (H/R) model of in vitro hepatocyte injury, supporting that the passive release of miR-192-5p represents a surrogate for hepatocyte death in liver injury. In critically ill patients, miR-192-5p levels were elevated selectively in patients with liver injury and closely correlated with the presence of hepatic injury. In contrast with up-regulated miR-192-5p in the serum, we detected a down-regulation of miR-192-5p in both injured mouse and human livers. Deregulation of miR-192-5p in livers was dependent on stimulation with TNF. Functional experiments confirmed a protective effect of down-regulation of miR-192-5p in hepatocytes, suggesting a role of miR-192-5p in limiting liver injury. Finally, we identified Zeb2, an important regulator of cell death, as a potential target gene mediating the function of miR-192-5p Our data suggest that miR-192-5p is involved in the regulation of liver cell death during acute liver injury and might represent a potent marker of hepatic injury., (© 2016 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society.)
- Published
- 2016
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63. Elevated Omentin Serum Levels Predict Long-Term Survival in Critically Ill Patients.
- Author
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Luedde M, Benz F, Niedeggen J, Vucur M, Hippe HJ, Spehlmann ME, Schueller F, Loosen S, Frey N, Trautwein C, Koch A, Luedde T, Tacke F, and Roderburg C
- Subjects
- Adiponectin blood, Adolescent, Adult, Aged, Aged, 80 and over, Biomarkers blood, Case-Control Studies, Critical Illness, Female, GPI-Linked Proteins blood, Humans, Male, Middle Aged, Receptors, Leptin blood, Sepsis pathology, Survival Analysis, Cytokines blood, Lectins blood, Sepsis blood
- Abstract
Introduction. Omentin, a recently described adipokine, was shown to be involved in the pathophysiology of inflammatory and infectious diseases. However, its role in critical illness and sepsis is currently unknown. Materials and Methods. Omentin serum concentrations were measured in 117 ICU-patients (84 with septic and 33 with nonseptic disease etiology) admitted to the medical ICU. Results were compared with 50 healthy controls. Results. Omentin serum levels of critically ill patients at admission to the ICU or after 72 hours of ICU treatment were similar compared to healthy controls. Moreover, circulating omentin levels were independent of sepsis and etiology of critical illness. Notably, serum concentrations of omentin could not be linked to concentrations of inflammatory cytokines or routinely used sepsis markers. While serum levels of omentin were not predictive for short term survival during ICU treatment, low omentin concentrations were an independent predictor of patients' overall survival. Omentin levels strongly correlated with that of other adipokines (e.g., leptin receptor or adiponectin), which have also been identified as prognostic markers in critical illness. Conclusions. Although circulating omentin levels did not differ between ICU-patients and controls, elevated omentin levels were predictive for an impaired patients' long term survival.
- Published
- 2016
- Full Text
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