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53 results on '"Liwen Liang"'

51. Three CCT domain-containing genes were identified to regulate heading date by candidate gene-based association mapping and transformation in rice

Author

Liwen Liang, Wen Yao, Haijiao Dong, Qiuping Li, Zhongmin Han, Li Zhang, Hu Zhao, Weibo Xie, Yongzhong Xing, Guangwei Li, Qin He, and Cong Tan

Subjects
Candidate gene, genetic structures, Genetic Linkage, Photoperiod, Molecular Sequence Data, Quantitative Trait Loci, Flowers, Biology, Quantitative trait locus, Genes, Plant, Article, Genetic linkage, Gene Expression Regulation, Plant, Genetic variation, Clustered Regularly Interspaced Short Palindromic Repeats, Amino Acid Sequence, Association mapping, Gene, Phylogeny, Plant Proteins, Regulation of gene expression, Genetics, Multidisciplinary, Plant Stems, food and beverages, Chromosome Mapping, Genetic Variation, Oryza, Plant Leaves, Transformation (genetics), RNA Interference, sense organs
Abstract

CCT domain-containing genes generally control flowering in plants. Currently, only six of the 41 CCT family genes have been confirmed to control flowering in rice. To efficiently identify more heading date-related genes from the CCT family, we compared the positions of heading date QTLs and CCT genes and found that 25 CCT family genes were located in the QTL regions. Association mapping showed that a total of 19 CCT family genes were associated with the heading date. Five of the seven associated genes within QTL regions and two of four associated genes outside of the QTL regions were confirmed to regulate heading date by transformation. None of the seven non-associated genes outside of the QTL regions regulates heading date. Obviously, combination of candidate gene-based association mapping with linkage analysis could improve the identification of functional genes. Three novel CCT family genes, including one non-associated (OsCCT01) and two associated genes (OsCCT11 and OsCCT19) regulated the heading date. The overexpression of OsCCT01 delayed flowering through suppressing the expression of Ehd1, Hd3a and RFT1 under both long day and short day conditions. Potential functions in regulating heading date of some untested CCT family genes were discussed.

Published
2014

53. Suppression of imaging crack caused by the gap between micromirrors in maskless lithography.

Author

Liwen Liang, Jinyun Zhou, Liang Lei, Bo Wang, Qu Wang, and Kunhua Wen

Subjects
*MICROMIRROR devices, *LITHOGRAPHY, *COHERENCE (Optics), *OPTICAL images, *LIGHTING
Abstract

The digital micromirror device (DMD) is the key device in maskless lithography. However, because of the machinery manufacturing limit of DMDs, the gap between the micromirrors may destroy the continuity of the graphic. This work presents a simple way to fill the imaging crack by controlling the partial coherence factor σ of the light source. A crack can be regarded as the image of a dark space. By considering the resolving power for such cracks under partially coherent illumination, the images of such dark spaces can be covered, preventing them from being imaged on the substrate. By using mathematical derivations of the light intensity distribution exposed to the substrate, and by utilizing the diffraction effect induced by the finite aperture of the optical projection system, an appropriate σ value can be determined for eliminating the image of the crack in an actual scene. The numerical simulation results demonstrate that this method can ensure the continuity of the graphic at the critical partial coherence factor σc regardless of the shape of the target graphic. [ABSTRACT FROM AUTHOR]

Published
2017
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