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In the title complex, [Mn(2)(C(15)H(13)N(2)O(3))(3)]ClO(4)·CH(3)OH, the two Mn(II) ions are bridged by two phenolate O atoms from two ligands, forming an Mn(2)O(2) quadrangle. Each Mn(II) ion has a distorted octa-hedral coordination geometry. One Mn(II) ion is coordinated by two N atoms and four O atoms from two ligands, and the other is coordinated by one N atom and five O atoms from three ligands. A dimer is formed by inter-molecular N-H⋯O hydrogen bonds. The dimers, perchlorate anions and methanol solvent mol-ecules are further connected into a chain along [01] through N-H⋯O and O-H⋯O hydrogen bonds.

Aim: To investigate the effect of Bu-Zhong-Yi-Qi-Tang (Decoction for Reinforcing Middle Jiao and Replenishing Qi) on deficiency of N-glycan/nitric oxide (NO) and islet damage induced by injecting two medium doses of streptozotocin (STZ)., Methods: Diabetes was induced by intraperitoneal injection of STZ at 55 mg/kg on day 1 and day 8. Islet damage was evaluated using a scoring system. Nitrite, nitrate, alpha-mannosidase and amylase activities were measured by colorimetry. N-glycan patterns of amylase were determined with lectin [ConA, pisum sativum agglutinin (PSA), peanut agglutinin (PNA), and lens culinaris agglutinin (LCA)] affinity precipitation method., Results: Severe islet necrosis and mild islet atrophy were observed in diabetic rats. The number and size of islets, the activities of alpha-mannosidase, amylase and nitrite were decreased, while the binding of PNA and LCA to amylase was increased. All of which were improved after treatment with Bu-Zhong-Yi-Qi-Tang. Islet damage was significantly correlated with nitrite, nitrate, alpha-mannosidase, amylase and the binding of LCA, PNA, and PSA to amylase., Conclusion: STZ-induced islet damage is related to N-glycan deficiency in proteins by blocking alpha-mannosidase activity and no deficiency, accumulation of unfolded proteins, and endoplasmic reticulum stress and activation of cellular signals, all of which are improved after treatment with Bu-Zhong-Yi-Qi-Tang.

Objective: To study the chemical constituents of Botrychium lanuginosum., Method: Various chromatographic techniques were used to isolate and purify the constituents. The structures were elucidated by chemical evidence and spectroscopic methods., Result: Ten compounds were isolated from the 95% ethanol extract of the herb of B. lanuginosum and their structures were elucidated as 30-nor-21beta-hopan-22-one (1), beta-sitosterol (2), luteolin (3), thunberginol A (4), apigenin (5), (6'-O-palmitoyl)-sitosterol-3-O-beta-D-glucoside (6), daucosterol (7), 1-O-beta-D-glucopyranosyl-(2S, 3R, 4E, 8Z)-2-[(2R-hydroxy hexadecanoyl) amino]-4, 8-octadecadiene-1, 3-diol (8), luteolin-7-O-glucoside (9), sucrose (10)., Conclusion: Compounds 1-10 were isolated from this genus for the first time.

Objective: To study the chemical constituents of the barks of Garcinia tetralata., Method: Compounds were isolated and purified repeatedly by silica gel and ODS column chromatography and their chemical structures were elucidated by their physicochemical properties and spectral data analysis., Result: Ten compounds were obtained and identified as 1, 7-dihydroxyxanthone (1), buchanaxanthone (2), garciniaxanthone H (3), 1, 2, 5-trihydroxyxanthone (4), subelliptenone H (5), 6-desoxyjacareubin (6), 6-deoxyisojacreubin (7), subelliptenone G (8), methyl orsellinate (9) and beta-sitosterol (10)., Conclusion: Compounds 1-10 were isolated from this plant and compound 9 was obtained from the genus Garcinia for the first time.

Along with the development of modern medicinal manufacture and pharmaceutical analysis technology, the precious Chinese patent medicine is also growing quickly. However, after the investigation of the dosage-forms and relative government standards, we find that the precious Chinese patent medicine needs more variety in administration and more precise and full-scale analysis standards based on the chemical materials because of the contradiction between the "global thoughts" logic in traditional Chinese medicine (TCM) theory and the "local study" of modern pharmaceutical analysis technology. Currently rising theories, metabonomics and coordination chemistry of TCM, with the superiority of entirety, can not only fulfill the requirement for " local study" but also accord with the "global thoughts" logic in TCM theory. They will be the reasonable guide for the development of the precious Chinese patent medicine.

A series of mutants of transglutaminase (TGase) gene from Streptomyces fradiae strains were obtained by low energy N+ ion beam irradiation, and the irradiation dose response curve was also investigated. Both the wild type and the mutants of TGase were compared by PCR-SSCP, and specific fragments were sequenced. Then the types of TGase gene mutation induced by N+ were characterized. The results show that the types of DNA base mutation included the transition, the transversion and the deletion. In all of the 24 bases of mutants, the base replacement occupies about 87.5% of the total mutants with only a small portion of gene deletion (12.5%). Interestingly, the frequency of the base transition (58.3%) is obviously higher than that of the base transversion (29.2%) among the base replacements. The base substitutions induced by N+ ion beam irradiation are C-->T transition, A-->G transition, G-->T transversion, C-->G transversion. Furthermore, mutations can be induced in all of the four DNA bases by low energy N+ ion beam irradiation, and cytosine shows the highest frequency of mutation.

Objective: To evaluate the effect of K2O addition on the crystallization property of dental glass-ceramics in the Li2O-SiO2-Al2O3-P2O5-ZnO system., Methods: Different content of K2O was added into Li2O-SiO2-Al2O3-P2O5-ZnO glass system. The heat-treated system of the glass-ceramics was determined by differential thermal analyses (DTA), then the crystallization components and the microstmcture of the glass-ceramics with different content of K2O were investigated from X-ray diffraction (XRD) analyses and scanning electron microscopy (SEM)., Results: Addition of K2O helped to reduce the viscosity of the glass system and improved crystallization. More lithium disilicate crystals appeared after heated-treatment of the glass system which contained 5.3 wt% addition of K2O, and the homogeneously lath-shaped crystals were 4 gm in length., Conclusion: Certain content of K2O can improve the crystallization property of dental glass-ceramics in the Li2O-SiO2-Al2O3-P2O5-ZnO system.