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Rhizosphere microbes in forests are key elements of the carbon sequestration of terrestrial ecosystems. To date, little is known about how the diversity and species interactions of the active rhizomicrobial community change during soil carbon sequestration and what interactions drive these changes. In this study, we used a combination of DNA and stable isotope method to explore correlations between the composition of microbial communities, N transformation, and the sequestration de novo of carbon in soils around Pinus tabuliformis and Quercus variabilis roots in North China. Rhizosphere soils from degraded lands, primary stage land (tree roots had colonized in degraded soil for 1 year), and nature forest were sampled for analyses. The results showed that microbial communities and newly sequestered soil organic carbon (SOC) contents changed with different tree species, environments, and successive stages. The fungal unweighted and weighted UniFrac distances could better show the different microbial species structures and differences in successive stages. Newly sequestered SOC was positively correlated with the bacterial order Rhizobiales (in P. tabuliformis forests), the fungal order Russulales (in Q. variabilis forests), and δ15N. Consequently, the bacterial order Rhizobiales acted as an important taxa for P. tabuliformis root-driven carbon sequestration, and the fungal order Russulales acted as an important taxa for Q. variabilis root-driven carbon sequestration. The two plant species allocated root exudates to different portion of their root systems, which in turn altered microbial community composition and function. The δ15N of soil organic matter could be an important indicator to estimate root-driven carbon sequestration. [ABSTRACT FROM AUTHOR]

Human cytomegalovirus (HCMV) enters host by glycoprotein B (gB)-mediated membrane fusion upon receptor-binding to gH/gL-related complexes, causing devastating diseases such as birth defects. Although an X-ray crystal structure of the recombinant gB ectodomain at postfusion conformation is available, the structures of prefusion gB and its complex with gH/gL on the viral envelope remain elusive. Here, we demonstrate the utility of cryo electron tomography (cryoET) with energy filtering and the cutting-edge technologies of Volta phase plate (VPP) and direct electron-counting detection to capture metastable prefusion viral fusion proteins and report the structures of glycoproteins in the native environment of HCMV virions. We established the validity of our approach by obtaining cryoET in situ structures of the vesicular stomatitis virus (VSV) glycoprotein G trimer (171 kD) in prefusion and postfusion conformations, which agree with the known crystal structures of purified G trimers in both conformations. The excellent contrast afforded by these technologies has enabled us to identify gB trimers (303kD) in two distinct conformations in HCMV tomograms and obtain their in situ structures at up to 21 Å resolution through subtomographic averaging. The predominant conformation (79%), which we designate as gB prefusion conformation, fashions a globular endodomain and a Christmas tree-shaped ectodomain, while the minority conformation (21%) has a columnar tree-shaped ectodomain that matches the crystal structure of the “postfusion” gB ectodomain. We also observed prefusion gB in complex with an “L”-shaped density attributed to the gH/gL complex. Integration of these structures of HCMV glycoproteins in multiple functional states and oligomeric forms with existing biochemical data and domain organization of other class III viral fusion proteins suggests that gH/gL receptor-binding triggers conformational changes of gB endodomain, which in turn triggers two essential steps to actuate virus-cell membrane fusion: exposure of gB fusion loops and unfurling of gB ectodomain. [ABSTRACT FROM AUTHOR]

The article focuses on the use of information and communication technology in the Ministry of Agriculture's five year plan. In order to develop the agriculture and rural economy and satisfy farmers' demand for production management and increase incomes, China, as a developing nation, has focused on its agricultural information services, engaged in a series of initiatives, and recorded some important achievements. With a view to strengthening the agricultural information network and advance the basis for a rural information service, the Ministry of Agriculture of China in September 2001 put forward the "Action Plan for Rural Market Information in the Tenth-Five Year Plan Period". The "Action Plan" stated that within 3-5 years, the network system for rural market information would be established across the country. It would cover provinces, cities, counties and most townships and serve leading enterprises in agricultural production, wholesale markets for agricultural products, intermediaries, and farming households that run their opera lion on a large scale.

Objective: To investigate the effect of obesity induced by high fat diet on decidual reaction of endometrium in mice, and the effect of high fat treatment on decidual reaction of endometrial stromal cells., Methods: Twelve 4-week-old healthy C57BL/6J female mice were randomly divided into high fat diet group and control group with 6 mice in each group. They were fed with high fat diet (22 kJ/g) or normal diet (16 kJ/g) for 12 weeks, respectively. The body weight of mice was measured every week. After feeding for 12 weeks, the body length and width of mice were measured, and the levels of fasting serum triglyceride and total cholesterol were determined. Then the mice were mated with healthy C57BL/6J male mice, and the uterine tissues were collected on the seventh day of pregnancy. The decidual cells and collagen fibers in mouse endometrium was observed by HE staining and Masson staining respectively. The expression of decidual reaction related proteins in mouse endometrium were detected by immunohistochemistry and Western blotting. Mouse endometrial stromal cells (mESCs) were isolated and treated with the oleic acid and palmitic acid in vitro , and the decidual reaction was induced with estradiol and progesterone. The accumulation of lipid droplets in mESCs was observed by oil red O and Bodipy staining. The cytoskeleton of mESCs was observed by phalloidin staining. The levels of decidual reaction related genes and proteins were detected by real-time fluorescence quantitative PCR and Western blotting., Results: After feeding for 12 weeks, the body weight of mice in the high fat group was significantly higher than that in the control group ( P <0.01), and there was no significant difference in body length between two groups ( P >0.05), but the body width of mice in the high fat group was significantly larger than that in the control group ( P <0.01), and the levels of serum triglyceride and total cholesterol were significantly higher than those in the control group (Both P <0.05). The number of embryo implantation in the high fat group was significantly less than that in the control group ( P <0.01). The differentiation of mESCs to decidual cells in high fat group was slow and abnormal. The expression levels of decidual reaction markers bone morphogenetic protein (BMP)2 and homeobox A10 (HOXA10) were lower than those in the control group, and there was significant difference in the expression level of HOXA10 ( P <0.01). The results of oil red O and Bodipy staining in mESCs showed that after high fat treatment, the accumulation of lipid droplets increased significantly, phalloidin staining showed abnormal cytoskeleton morphology. The expression levels of decidual reaction related genes dtprp , HOXA10 and proteins BMP2, HOXA10 and cyclooxygenase (COX)2 were significantly lower than those in the control group ( P <0.05)., Conclusion: Obesity induced by high fat diet and high fat treatment can impair the decidual reaction of endometrium and endometrial stromal cells in mice.

Classical swine fever (CSF) is an economically important disease of pigs caused by classical swine fever virus (CSFV). The live attenuated vaccine C-strain (also called HCLV strain) against CSF was produced by multiple passages of a highly virulent strain in rabbits. However, the molecular determinants for its attenuation and protection remain unclear. In this study, we identified a unique glycosylation at position 986 ( 986 NYT 988 ) on the E2 glycoprotein Domain IV of C-strain but not ( 986 NYA 988 ) the highly virulent CSFV Shimen strain. We evaluated the infectivity, virulence, and protective efficacy of the C-strain-based mutant rHCLV-T988A lacking the glycosylation and Shimen strain mutant rShimen-A988T acquiring an additional glycosylation at position 986. rShimen-A988T showed a significantly decreased viral replication ability in SK6 cells, while rHCLV-T988A exhibited a growth kinetics indistinguishable from that of C-strain. Removal of the C-strain glycosylation site does not affect viral replication in rabbits and the attenuated phenotype in pigs. However, rShimen-A988T was attenuated and protected the pigs from a lethal challenge at 14 days postinoculation. In contrast, the rHCLV-T988A-inoculated pigs showed transient fever, a few clinical signs, and pathological changes in the spleens upon challenge with the Shimen strain. Mechanistic investigations revealed that the unique glycosylation at position 986 influences viral spreading, alters the formation of E2 homodimers, and leads to increased production of neutralizing antibodies. Collectively, our data for the first time demonstrate that the unique glycosylation at position 986 on the E2 glycoprotein is responsible for viral attenuation and protection. IMPORTANCE Viral glycoproteins involve in infectivity, virulence, and host immune responses. Deglycosylation on the E rns , E1, or E2 glycoprotein of highly virulent classical swine fever virus (CSFV) attenuated viral virulence in pigs, indicating that the glycosylation contributes to the pathogenicity of the highly virulent strain. However, the effects of the glycosylation on the C-strain E2 glycoprotein on viral infectivity in cells, viral attenuation, and protection in pigs have not been elucidated. This study demonstrates the unique glycosylation at position 986 on the C-strain E2 glycoprotein. C-strain mutant removing the glycosylation at the site provides only partial protection against CSFV challenge. Remarkably, the addition of the glycan to E2 of the highly virulent Shimen strain attenuates the viral virulence and confers complete protection against the lethal challenge in pigs. Our findings provide a new insight into the contribution of the glycosylation to the virus attenuation and protection.

Objective: To investigate the dietary exposure level of advanced glycation end products(AGEs) in the diet of Shenzhen residents., Methods: 3 markets, 6 supermarkets and 10 chain catering units in Shenzhen were selected as sampling points. 196 food samples were collected in 11 categories in batches from December 2016 to October 2017. The AGEs content database was obtained by detecting carboxy methyl lysine by ELISA competition method. Combined with the food consumption data of Shenzhen residents in the 2011 survey of dietary and nutritional status of Shenzhen residents, through Monte Carlo simulation, the probability distribution of AGEs dietary exposure was calculated by using the Latin hypercube method from the AGEs content data and consumption data, and the result were expressed by the exposure corresponding to different percentiles(P50 and P95)., Results: In Shenzhen, 50% of the population had a dietary exposure of more than 37. 2 mg/d per person, while 5% of the population had a dietary exposure of more than 65. 9 mg/d per person. The first three factors that had a great impact on the dietary exposure of AGEs were the AGEs content of cereal and its products, the AGEs content of meat and its products, and the consumption of cereal and its products. The top three sources of AGEs exposure for both P50 and P95 were cereal and its products and its products taste, meat and its products., Conclusion: 5% of Shenzhen residents had a high intake of AGEs, which mainly came from cereals and their products, condiments, meat and their products.

Rhizosphere microbes in forests are key elements of the carbon sequestration of terrestrial ecosystems. To date, little is known about how the diversity and species interactions of the active rhizomicrobial community change during soil carbon sequestration and what interactions drive these changes. In this study, we used a combination of DNA and stable isotope method to explore correlations between the composition of microbial communities, N transformation, and the sequestration de novo of carbon in soils around Pinus tabuliformis and Quercus variabilis roots in North China. Rhizosphere soils from degraded lands, primary stage land (tree roots had colonized in degraded soil for 1 year), and nature forest were sampled for analyses. The results showed that microbial communities and newly sequestered soil organic carbon (SOC) contents changed with different tree species, environments, and successive stages. The fungal unweighted and weighted UniFrac distances could better show the different microbial species structures and differences in successive stages. Newly sequestered SOC was positively correlated with the bacterial order Rhizobiales (in P. tabuliformis forests), the fungal order Russulales (in Q. variabilis forests), and δ 15 N. Consequently, the bacterial order Rhizobiales acted as an important taxa for P. tabuliformis root-driven carbon sequestration, and the fungal order Russulales acted as an important taxa for Q. variabilis root-driven carbon sequestration. The two plant species allocated root exudates to different portion of their root systems, which in turn altered microbial community composition and function. The δ 15 N of soil organic matter could be an important indicator to estimate root-driven carbon sequestration., (Copyright © 2020 Song, Tong, Liu and Li.)

Despite reports of successful clinical cases, many tumors appear to resist infection by oncolytic viruses (OVs). To circumvent this problem, an armed vesicular stomatitis virus was constructed by inserting a transgene to express Smac/DIABLO during virus infection (VSV-S). Endogenous Smac in HeLa cells was diminished during wtVSV infection, whereas the Smac level was enhanced during VSV-S infection. Apoptosis was readily induced by VSV-S, but not wtVSV, infection. More importantly, the tumor volume was reduced to a larger extent when xenografts of 4T1 cells in BALB/c mice and OV-resistant T-47D cells in nude mice were intratumorally injected with VSV-S. VSV-S represents a novel mechanism to overcome tumor resistance, resulting in more significant tumor regression due to enhanced apoptosis.

Organic anion transporting polypeptides (OATPs; gene symbol SLCO ) are membrane transporters that mediate the transport of wide ranges of compounds. The expression of different OATP members has been reported in the kidney, liver, placenta, brain, and intestine. Because of their broad substrate spectra and wide distribution within the human body, these transporters have been proposed to play key roles in the influx transport of many oral drugs. Inflammation is known to regulate the expression and functions of many drug-metabolizing enzymes and drug transporters. As a proinflammatory cytokine, tumor necrosis factor- α (TNF α ) has been shown to affect the expression of different drug transporters, including OATP family members. In the present study, a putative nuclear factor- κ B (NF κ B) binding site ranging from -1845 to -1836 was identified at the proximal promoter region of OATP1A2 coding gene SLCO1A2 Electrophoretic mobility shift assays and chromatin immunoprecipitation showed that nuclear extracts from both breast cancer cell MCF7 and liver cancer cell HepG2 interacted with an oligonucleotide probe containing the putative NF κ B binding site and that the DNA-protein complexes contained both p65 and p50 subunits of NF κ B. Further study revealed that the binding site may be responsible in part for the suppression effect of TNF α toward SLCO1A2 expression because the treatment of TNF α significantly increased. Treatment of TNF α significantly increased formation of the DNA-protein complexes and mutations at essential bases of the putative NF κ B binding site abolished responsiveness to the TNF α neutralizing antibody, suggesting that the binding site may be responsible in part for the suppression effect of TNF α towars SLCO1A2 expression., (Copyright © 2018 by The American Society for Pharmacology and Experimental Therapeutics.)