705 results on '"Leucócitos"'
Search Results
52. Evaluación de la Actividad Anti-inflamatoria de Propóleos Chileno sobre Cortes Histológicos de Orejas de Ratón.
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Núñez R., Daniela, Balboa P., Natalia, Alvear Z., Marysol, Ceron N., Anabelle, Abarzua S., Karen, and Vasconcellos C., Adriana
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Propolis is a complex resinous product produced by bees Apis mellifera, which has a number of biological activities such as an immunomodulator, anti-inflammatory, anticarcinogenic, antiviral, antibacterial, antioxidant, among others. The purpose of the following study was to perform an in vivo evaluation of the anti-inflammatory properties of a Chilean propolis extract, on the model of atrial edema induced 12-O-tetradecanoyl phorbol-13- acetate (TPA) in the mouse auricular pavilion, for later evaluation and histological analysis. The Chilean propolis extract (EPP) used was obtained from an ethanolic, rotaevaporated and lyophilized macerate. It was observed that the EPP significantly decreased edema and inflammatory infiltrate. These results suggest that the ethanolic extract of Chilean propolis possesses potential anti-inflammatory or modulatory effects of the immune system in atrial edema. [ABSTRACT FROM AUTHOR]
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- 2018
53. Dietary lysine: calorie ratio on performance, blood parameters and apparent nutrient digestibility in pigs from 50 to 70 kg.
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Gandra, E. R. S., Gandra, J. R., Budiño, F. H. L., Donato, D. C. Z., Garcia, P. D. S. R., Berto, D. A., and Trindade Neto, M. A.
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LYSINE in animal nutrition , *CALORIC content of foods , *SWINE nutrition - Abstract
Three trials occured to estimate the optimal dietary level of lysine (Lys) and its ratio with metabolizable energy (ME) using pigs from 50 to 70 kg BW. The first with 72 barrows (49.75±0.41 kg initial BW) and second with 72 gilts (46.05±0.38 kg intial BW). Dietary levels of Lys were: 7.00, 8.00, 9.00, 10.00 11.00 and 12.00 g/kg, and 14.25 MJ/kg ME. The thirth with 12 barrows and 12 gilts were fed with four diets (7.00, 9.00, 10.00 and 12.00 Lys g/kg) from the performance trial to evaluate apparent nutrient digestibility. In the first trial, there was a linear improve on feed conversion ratio, crude protein intake (g/day) (CPI) and crude protein efficiency (CPE) of the gilts from 0 to 16 days. From 0 to 32 days dietary lysine levels continued to show linear increase in CPI and CPE and a quadratic effect on relative weight gain (RWG) and FCR of the gilts. There was linear increase on CPE for barrows from 0 to 16 days and quadratic effect on CPI from 0 to 32 days. Data from digestibility and metabolism assay did not show interaction between sex as effect of Lys, although N retention (g) was higher in gilts than barrows. Therefore, it was concluded that for gilts and barrows from 50 to 70 kg, the recommended levels were 10.14 and 7.00 g/kg Lys or 0.71 and 0.49 g Lys/MJ ME, respectively. [ABSTRACT FROM AUTHOR]
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- 2018
54. Haemogram, blood viscosity and osmotic fragility of Isa White and Barred Plymouth Rock cocks fed varying levels of salt.
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Aro, S. O.
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BLOOD cell count , *BLOOD viscosity , *PLYMOUTH Rock chicken - Abstract
This experiment was performed to determine the effect of diets with varying salt levels on the haemogram, whole blood viscosity and osmotic fragility of Barred Plymouth Rock (BPR) and Isa White (IW) breeding cocks. Four experimental diets designated as: T1 (control diet with 0.25 % salt), T2 (diet with 0.50 % salt), T3 (diet with 0.75 % salt) and T4 (diet with 1.00 % salt) were fed to 48 cocks of the two different breeds grouped into 12 birds per treatment and at four birds per replicate. At the end of eight weeks of experiment, blood samples were collected for the determination of the haemogram, whole blood viscosity and red blood cell osmotic fragility. The results revealed differences (P<0.05) in erythrocyte sedimentation rate, haemoglobin content, red blood corpuscles, packed cell volume and whole blood viscosity between the two breeds. Also, percentage eosinophils (3.30±0.15 versus 3.10±0.10) and basophils (1.60±0.16 versus 1.80±0.13) differed (P<0.05) between breeds. The red blood cells of birds on the high salt diets were more osmotically fragile than the control diet. There was no breed difference in the osmotic fragility between the BPR and IW cocks, however the BPR breed was more sensitive to osmotic fragility at all saline concentrations and dietary levels than the IW breed. Conclusively, increase in the dietary salt up to 0.75 % level has no significant effect on the two commercial breeding cocks as far as their differential leucocyte count is concerned, but a case of sub-clinical basophilia could be induced at 1.00% level. Also, the IW seemed the more osmotically stable breed in terms of its erythrocytic osmotic fragility. [ABSTRACT FROM AUTHOR]
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- 2018
55. EFECTO ANTIIFLAMATORIO DE LA FRACCIÓN FLAVONOIDE DE Lepechinia meyenii (Walp.) Epling (SALVIA) SOBRE LEUCOCITOS DE PACIENTES CON ARTRITIS REUMATOIDE.
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Arenas-Chavez, Carlos Alberto, Wiche-Salinas, Tomas, Valencia-Mercado, Ignacio, Calle-Valdez, Ronald, Vera-Gonzales, Corina, Malaga-Contreras, Stephanie, Flores-Rivera, Fernando, and Huanqui-Guerra, Carlos
- Abstract
Objectives. to assess the anti-inflammatory effect of the flavonoid fraction of Lepechinia meyenii (Walp.) Epling on leukocytes of patients with rheumatoid arthritis (RA). Materials and Methods. Plants of the species Lepechinia meyenii (Walp.) Epling were collected and then different flavonoid fractions were extracted by column and thin layer chromatography. The superoxide anion production was evaluated by means of the reduction of nitroblue tetrazolium assay technique in neutrophils obtained from the blood of patients with RA, divided into three groups: negative control, which consisted of neutrophils (5x105 cells); positive control, made up of PMA (phorbol myristate acetate)-activated neutrophils (150 ng/mL), and the treatments, comprised of neutrophils activated and treated with different concentrations of the flavonoid fraction LM8 (60, 120, and 180 ug/mL). The expression of proinflammatory genes was studied by RTqPCR in mononuclear leukocytes obtained from patients with RA, divided into three groups: negative control, which consisted of mononuclear leukocytes (5x105 cells); positive control, made up of phytohaemagglutinin (PHA) (150 ug/ml)-activated mononuclear leukocytes, and the treatment, comprised of mononuclear leukocytes activated and treated with the flavonoid fraction LM8 (120 ug/mL). Results. Several flavonoid fractions were purified, with fraction LM8 showing the best immunomodulating effect. Said fraction diminished the superoxide anion production dependent on concentration. On the other hand, it diminished the expression of TNFα, IL8, and IL17 in mononuclear leukocytes. Conclusions. : Rheumatoid Arthritis, Salvia, leukocytes, anion superoxide, proinflammatory genes . [ABSTRACT FROM AUTHOR]
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- 2018
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- View/download PDF
56. White blood cell count of patients with oral squamous cell carcinoma at the time of diagnosis: a cross-sectional retrospective study of a population from southern Brazil.
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de Antoni Brunischaki, Kelem, Camara Brew, Myrian, Serra Bavaresco, Caren, and Quevedo Miguens Jr, Sergio Augusto
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PEOPLE with alcoholism ,ALVEOLAR process ,CANCER patients ,DENTISTRY ,EPIDEMIOLOGY ,GINGIVA ,LEUCOCYTES ,LYMPHOCYTES ,MOUTH ,MOUTH tumors ,NEUTROPHILS ,ORAL medicine ,SMOKING ,SQUAMOUS cell carcinoma ,WHITE people ,WOUNDS & injuries ,RESIDENTIAL patterns ,SECONDARY analysis ,CROSS-sectional method ,RETROSPECTIVE studies ,LEUKOCYTE count - Abstract
Copyright of Revista Odonto Ciencia is the property of EDIPUCRS - Editora Universitaria da PUCRS and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2018
- Full Text
- View/download PDF
57. Haematological assessment of four amazonian ornamental armoured catfish (Teleostei, Loricariidae).
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de Souza Neves, Mikaelle, Barbas, Luis André Luz, do Couto, Márcia Valéria Silva, Lopes, Josiane Nazaré Silva, Tavares-Dias, Marcos, and Fujimoto, Rodrigo Yudi
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Copyright of Acta Scientiarum: Biological Sciences is the property of Universidade Estadual de Maringa and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2018
- Full Text
- View/download PDF
58. Age‐based inter‐subject variability in platelet and white blood cell concentrations of platelet‐rich plasma prepared using a new application to blood separation system
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Marta Elena Losa-Iglesias, Bibiana Trevisson, Natalia González, Daniel López-López, David Sevillano, Luis Alou, and Ricardo Becerro-de-Bengoa-Vallejo
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Blood Platelets ,Male ,leukocytes ,Population ,Physiology ,Dermatology ,Blood cell ,030207 dermatology & venereal diseases ,03 medical and health sciences ,Plasma rico en plaquetas ,Leukocyte Count ,0302 clinical medicine ,Platelet-rich plasma ,blood ,White blood cell ,medicine ,Leukocytes ,Humans ,Platelet ,030212 general & internal medicine ,Leucocitos ,education ,Whole blood ,Aged ,education.field_of_study ,business.industry ,Platelet-Rich Plasma ,Blood separation ,Sangre ,Original Articles ,Healthy Volunteers ,medicine.anatomical_structure ,Blood ,Ageing ,Surgery ,Original Article ,Female ,business ,platelet‐rich plasma - Abstract
The benefit of autologous platelet‐rich plasma (PRP) treatment is still under discussion. Variations in PRP products, consequence of the lack of a standardised protocol for the multitude of commercially available blood separation systems and the lack of knowledge of the optimal composition of PRP or its suitability for the proposed indication are some of the reasons behind clinical inconsistencies. The impact of inter‐subject variability in PRP has received less attention in comparison. The purpose of this study was to determine the inter‐subject variability, based on age, in the concentrates prepared by a new blood concentration system. Twenty‐six healthy volunteers of both genders (29‐93 years) were enrolled. Whole blood (WB) was collected from each participant to prepare PRP using the Easy PRP kit. Platelets and white blood cells (WBC) from WB and PRP were analysed after split population by age; patients younger than 65 years (n = 13) and patients ≥65 years old (n = 13). Among the demographic characteristics tested, only age was significantly different between the groups. Cell capture efficiency of the system was specific for each type of blood cell and identical for both age groups. Platelets and WBC in PRP were higher than in WB (P
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- 2021
59. AVALIAÇÃO DO CONCENTRADO DE HEMÁCIAS DE CÃES APÓS LEUCORREDUÇÃO.
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Massae Nakage Canesin, Ana Paula, Ribeiro Garcia, Marilia, and Sangaletti Terçariol, César Augusto
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Copyright of Acta Veterinaria Brasilica is the property of Acta Veterinaria Brasilica and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
60. Detección de Chlamydia spp. en leucocitos de sangre periférica de ovinos sin aborto previo
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Guerra-Infante, Fernando Martin, López-Villegas, Edgar Oliver, López-Vargas, Susan, Mata-Vite, Carlos, López Hurtado, Marcela, Cancino-Díaz, Juan Carlos, Gutiérrez-Paredes, Sonia, Haro Cruz, María de Jesús de, Guerra-Infante, Fernando Martin, López-Villegas, Edgar Oliver, López-Vargas, Susan, Mata-Vite, Carlos, López Hurtado, Marcela, Cancino-Díaz, Juan Carlos, Gutiérrez-Paredes, Sonia, and Haro Cruz, María de Jesús de
- Abstract
The Ewes Enzootic Abortion is a disease caused byChlamydia abortus. It is deemed a zoonosis and one of the leading causes of financial losses in this type of business. This article focuses on using the culture of leukocytes from animals without any abortion symptoms and antibody detection to determine the potential presence ofC. abortusin backyard exploitations. Forty-two samples of peripheral blood were obtained from ewes in different populations. The detection ofChlamydia abortuswas carried out by using the Giemsa dye and PCR technique. Anti-C. Abortusantibody detection was performed through a commercial ELISA technique. Results showed 21 positive samples using the PCR, and only ten were positive according to the Giemsa dye, while 22 serum samples showed anti-C. abortusantibody. In this study, 38.1% of the samples were positive for theC. abortusinfection, as verified with the PCR and serology. In conclusion, peripheral blood leukocytes can be helpful to detect an infection caused byChlamydiaspp. Animal exploitation without any previous abortion allows knowing the real prevalence of ewes’ enzootic abortion in Mexico., Resumen El aborto enzootico ovino es una enfermedad causada por Chlamydia abortus. Es considerada una zoonosis y una de las principales causas de pérdidas económicas en estas explotaciones. Este trabajo se enfocó en utilizar el cultivo de leucocitos de animales sin signos de abortos y la detección de anticuerpos para determinar la posible presencia de C. abortus en explotaciones de traspatio. Se obtuvieron 42 muestras de sangre periférica de ovejas de diferentes poblaciones. La detección de Chlamydia abortus se realizó mediante la tinción de Giemsa y la técnica de PCR. La detección de anticuerpos anti-C. abortus se dio mediante una técnica de ELISA comercial. Los resultados mostraron 21 muestras positivas mediante la técnica de PCR, de las cuales solo 10 fueron positivas mediante la técnica de Giemsa, mientras que 22 sueros mostraron anticuerpos anti-C. abortus. En este estudio el 38,1 % de las muestras fueron positivas a la infección por C. abortus, como se confirmó mediante PCR y serología. En conclusión, los leucocitos de sangre periférica pueden ser útiles para detectar una infección por Chlamydia spp. en explotaciones sin historial de abortos, con lo que se puede conocer la prevalencia real del aborto enzootico ovino en México.
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- 2022
61. Influence of Sexual Dimorphism, Aging, and Differential Cell Capture Efficiency of Blood Separation Systems on the Quality of Platelet-Rich Plasma
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López-López, Daniel, Trevissón Redondo, Bibiana, Becerro-de-Bengoa-Vallejo, Ricardo, Sevillano Fernández, David, González Hidalgo, Natalia, Losa Iglesias, Marta Elena, Alou, Luis, López-López, Daniel, Trevissón Redondo, Bibiana, Becerro-de-Bengoa-Vallejo, Ricardo, Sevillano Fernández, David, González Hidalgo, Natalia, Losa Iglesias, Marta Elena, and Alou, Luis
- Abstract
[Abstract] Few studies have checked the impact of the hormonal/immunological dimorphism of patients on the cellular composition of platelet-rich plasma products (PRP). Whole blood (WB) from 26 volunteers was concentrated using a device previously characterized. Platelet and white blood cell (WBC) counts in WB and PRP were compared between genders, and after the population was split into pre (≤50 years)- and post (>50 years)-menopausal ages. In WB, platelet-total WBC densities were comparable in men and women. The phagocytic cell composition differed (p ≤ 0.04). After dividing by ages, phagocytic cell discrepancies were linked to women > 50 years (p ≤ 0.05), and differences emerged in lymphocyte counts (lower in >50 years groups, within and between genders, p ≤ 0.05). In PRP, densities were significantly higher, but the PRP/WB ratios varied according to blood cell (lower for phagocytic cells) and between subjects (more favorable at a lower density of a particular blood cell in WB). This "system compensatory efficiency" reduced/reverted PRP differences in the leukocyte composition between genders/age-sex groups in WB. In PRP, neutrophils were higher in younger men than older women (p < 0.05). WB lymphocyte differences between age-sex groups persisted. Age is a more determining factor than sex in the preparation of PRP. Post-menopause, sexual dimorphism strongly influences the composition of leukocytes, also conditioned by the capture efficiency of the system.
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- 2022
62. Leukocyte count and neutrophillymphocyte ratio in the second trimester of pregnancy as a predictor of preeclampsia
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Reyna-Villasmil, Eduardo, Torres-Cepeda, Duly, Mejía-Montilla, Jorly, Reyna-Villasmil, Nadia, Rondón-Tapia, Martha, and Fernández-Ramírez, Andreina
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Neutrófilos ,Relación neutrófilos/linfocitos ,Neutrophils ,Predicción ,Neutrophil/lymphocyte ratio ,Leukocytes ,Leucocitos ,General Medicine ,Preeclampsia ,Prediction - Abstract
RESUMEN Objetivo : Establecer la utilidad del contaje de leucocitos y relación neutrófilos/ linfocitos en el segundo trimestre del embarazo como predictor del desarrollo de preeclampsia. Diseño: Estudio de casos-controles. Institución. Hospital Central “Dr. Urquinaona”, Venezuela. Participantes : Embarazadas entre 17 y 20 semanas que acudieron a la consulta prenatal y fueron seguidas hasta el término del embarazo. Métodos: Se tomaron muestras de sangre de las gestantes, que fueron seguidas hasta el parto para establecer si desarrollaban preeclampsia. Principales medidas de resultado. Características generales de las pacientes, valores de leucocitos, neutrófilos, linfocitos, relación neutrófilos/linfocitos y eficacia pronóstica. Resultados : De las 504 pacientes seleccionadas, 41 embarazadas desarrollaron preeclampsia (grupo A) y 463 embarazadas fueron consideradas como controles (grupo B). No se encontraron diferencias estadísticamente significativas en las características generales al inicio del estudio (p = ns). La edad gestacional en el momento del diagnóstico de preeclampsia en el grupo A fue de 35,0 ± 3,2 semanas. Se encontró que las pacientes del grupo A presentaron valores significativamente más elevados de leucocitos, neutrófilos y relación neutrófilos/linfocitos junto a valores más bajos de linfocitos comparados con las pacientes del grupo B (p < 0,05). Solo los valores absolutos de neutrófilos (área bajo la curva 0,810) y de relación neutrófilos/linfocitos (área bajo la curva 0,963) tuvieron valores pronósticos útiles para discriminar entre los grupos de pacientes para el desarrollo de preeclampsia (p < 0,05). Conclusión: La relación neutrófilos/linfocitos y el contaje absoluto de neutrófilos en el segundo trimestre del embarazo son herramientas útiles en la predicción de preeclampsia. ABSTRACT Objective : To establish the usefulness of leukocyte count and neutrophil/lymphocyte ratio in the second trimester of pregnancy as a predictor of the development of preeclampsia. Design : Case-control study. Institution: Hospital Central "Dr. Urquinaona", Venezuela. Participants : Pregnant women between 17 and 20 weeks who attended prenatal consultation and were followed up to term. Methods: Blood samples were taken from the pregnant women who were followed until delivery to establish whether they developed preeclampsia. Main outcome measures: General characteristics of the patients, values of leukocytes, neutrophils, lymphocytes, neutrophil/lymphocyte ratio and prognostic efficacy. Results : Of the 504 patients selected, 41 pregnant women developed preeclampsia (group A) and 463 pregnant women were considered as controls (group B). No statistically significant differences were found in overall characteristics at baseline (p = ns). The gestational age at the time of diagnosis of preeclampsia in group A was 35.0 +/3.2 weeks. Patients in group A were found to have significantly higher values of leukocytes, neutrophils and neutrophil/lymphocyte ratio along with lower values of lymphocytes compared to patients in group B (p < 0.05). Only the absolute values of neutrophils (area under the curve 0.810) and neutrophil/lymphocyte ratio (area under the curve 0.963) had useful prognostic values for discriminating between patient groups for the development of preeclampsia (p < 0.05). Conclusion: The neutrophil/lymphocyte ratio and absolute neutrophil count in the second trimester of pregnancy are useful tools in the prediction of preeclampsia.
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- 2022
63. Introducción de metodología docente multimedia en las prácticas de hematología: recuento de células sanguíneas mediante el uso de la cámara Neubauer
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Pineda Merlo, Begoña, Pascual Mora, Maria, Lloret Alcañiz, Ana, Obrador Pla, Elena, and Piqueras Franco, Marta
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Multimedia teaching methodology ,Blood cell count ,Erythrocytes ,Metodología docente multimedia ,Video ,Eritrocitos ,Clase práctica ,Practical class ,Innovación ,Cámara Neubauer ,Leukocytes ,Leucocitos ,Neubauer chamber ,Recuento de células sanguíneas ,Innovation - Abstract
[EN] Blood cell count is a technique widely used both in clinical and in research, and is essential in the area of health in order to identify the presence of hematological alterations. With the aim of incorporating teaching innovation and improvement methods in the Hematology practices of the Physiology subject, in the Medicine Degree and in different degrees of Health Sciences area, we opted for the use of multimedia material based on the projection of a video that include the following aspects: 1) Introduction of the basis of the blood cell count. 2) Structure and use of the Neubauer camera. 3) Manipulation and preparation of blood samples. 4) Management of the microscope. 5) Count and calculation of the concentration of erythrocytes and leukocytes in the sample. 6) Interpretation of values. The expected results with the introduction of this methodology are: 1) Strengthen the understanding of Physiology concepts related to practice. 2) Optimize class time improving the development of the session. 3) Maintain and improve the attention, motivation, reflection and actions of the students in the laboratory., [ES] El recuento de células sanguíneas es una técnica muy utilizada de forma rutinaria, tanto en clínica como en investigación, y resulta imprescindible en el área de la salud para poder identificar la presencia de alguna alteración a nivel hematológico. Con el objetivo de incorporar métodos de innovación y mejora docente en las prácticas de Hematología de la asignatura de Fisiología, impartida en el Grado de Medicina y en distintos grados del área de las Ciencias de la Salud, optamos por el uso de material multimedia basado en la proyección de un vídeo que recoge los siguientes aspectos: 1) Introducción del fundamento del recuento celular sanguíneo. 2) Estructura y uso de la cámara Neubauer. 3) Manipulación y preparación de muestras de sangre. 4) Manejo del microscopio. 5) Recuento y cálculo de la concentración de eritrocitos y leucocitos en la muestra. 6) Interpretación de los valores. Los resultados previstos de la introducción de esta metodología son: 1) Reforzar la comprensión de los conceptos de Fisiología referentes a la práctica. 2) Optimizar el tiempo en clase repercutiendo positivamente en el desarrollo de la sesión. 3) Mantener y mejorar la atención, la motivación, la reflexión y las acciones del alumnado.
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- 2022
64. Efeito da Fibrina Leucoplaquetária Autóloga (FLA) na reparação de lesões cutâneas crônicas : estudo piloto
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Lopes, Fátima Cristina Alves Sicca and Kückelhaus, Selma Aparecida Souza
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Plaquetas ,Reparação tecidual ,Leucócitos ,Fibrina Leucoplaquetária Autóloga ,Comorbidades - Abstract
Dissertação (mestrado) — Universidade de Brasília, Faculdade de Medicina, Programa de Pós-Graduação em Ciências Médicas, 2022. Introdução: O tratamento de lesões crônicas acarreta elevados custos aos sistemas de saúde, como também reduz a qualidade de vida das pessoas por elas acometidas. Visando a melhoria no tratamento de lesões crônicas é possível que o uso da FLA proporcione resposta clínica favorável, com maior precocidade no reparo tecidual, uma vez que os concentrados plaquetários autólogos podem potencializar a estimulação do processo fisiológico da cicatrização e auxiliar na regeneração de diversos tecidos. Objetivo: Avaliar o efeito da aplicação tópica semanal da fibrina leucoplaquetária autóloga no processo de reparação de lesões crônicas. Metodologia: Trata-se de um estudo clínico experimental, controlado, randomizado, descritivo, comparativo e sem cegamento, realizado no Serviço Ambulatorial de Enfermagem em Estomaterapia (SAEE) do Hospital Universitário de Brasília, UnB – DF. O estudo foi composto por dois grupos, sendo eles: o grupo Controle (n=15) e o grupo Intervenção / FLA (n=15). Os parâmetros qualitativos e quantitativos das lesões de ambos os grupos eram obtidos pelo mesmo observador, uma vez por semana e ao longo de três meses. Nas análises qualitativas foram considerados os tecidos expostos (superficiais e/ou profundos), sua mensuração (área, perímetro e borda das lesões), as características macroscópicas das lesões e a evolução do desfecho (cura das lesões). Resultados: A análise dos dados mostrou que: 1) houve predomínio de mulheres em ambos os grupos, controle e FLA; 2) não houve diferenças entre os grupos quanto à faixa etária, índice de massa corporal, total de lesões, etiologia das lesões e presença de comorbidades; 3) o tratamento convencional ou FLA, bem como o tempo de tratamento não afetaram as características macroscópicas das lesões relativas à presença e intensidade da dor, odor, borda das lesões, área perilesão, exsudato; 4) o tratamento com a FLA aumentou o percentual de epitelização das lesões com 4, 8 ou 12 semanas de tratamento e com 8 semanas para o grupo controle; 5) o tratamento convencional não afetou o tecido de granulação, enquanto que no grupo tratado com a FLA houve maior percentual de tecido de granulação com 12 semanas; 6) não houve diferença na área das lesões para os indivíduos do grupo tratado convencionalmente, enquanto que para o grupo tratado com a FLA houve redução na área das lesões decorridos 4 ou 8 semanas do tratamento; 7) a FLA aumentou o percentual de indivíduos que tiveram redução na área das lesões, mas não alterou o tempo para o fechamento das lesões/cura até 12 semanas de tratamento. Conclusão: O conjunto dos resultados indicou que a fibrina leucoplaquetária autóloga favorece o processo de cicatrização/reparação de lesões crônicas vasculares antecipando a cura. Por se tratar de um tratamento de baixo custo e de performance potencialmente superior aos tratamentos convencionais, pode diminuir o quadro álgico dos indivíduos tratados com consequente melhoria na qualidade de vida desses pacientes. Introduction: The treatment of chronic injuries entails high costs for health systems, as well as reducing the life quality of people affected by them. Aiming at improving the treatment of chronic wounds, it is possible that the use of L-PRF provides a favorable clinical response, with greater precocity in tissue repair, since autologous platelet concentrates can potentiate the stimulation of the physiological healing process and assist in the regeneration of various tissues. Objective: Evaluate the effect of weekly topical application of Leukocyte- and Platelet-Rich Fibrin in the repair process of chronic wounds. Methodology: This is an experimental, controlled, randomized, descriptive, comparative and unblinded clinical study, carried out at the Outpatient Service of Nursing in Stomatherapy (SAEE) of the University Hospital of Brasília, UnB - DF. The study was composed of two groups, namely: Control group (n=15) and Intervention/L-PRF group (n=15). The qualitative and quantitative parameters of the wounds in both groups were obtained by the same observer, once a week and for three months. In the qualitative analysis, the exposed tissues (superficial and/or deep), their measurement (area, perimeter and edge of the wounds), the macroscopic characteristics of the wounds and the evolution of the outcome (healing of the wounds) were considered. Results: Data analysis showed that: 1) there was a predominance of women in both groups, control and L-PRF; 2) there were no differences between the groups in terms of age, body mass index, total number of wounds, etiology of lesions and presence of comorbidities; 3) conventional or L-PRF treatment, as well as treatment time, did not affect the macroscopic characteristics of the wounds regarding the presence and intensity of pain, odor, lesion edges, periwound area, exudate; 4) treatment with L-PRF increased the percentage of epithelialization of wounds at 4, 8 or 12 weeks of treatment and at 8 weeks for the control group; 5) the conventional treatment did not affect the granulation tissue, while in the group treated with L-PRF there was a higher percentage of granulation tissue at 12 weeks; 6) there was no difference in the wound’s area for individuals in the conventional treatment group, while for the group treated with L-PRF there was a reduction in the area of wounds after 4 or 8 weeks of treatment; 7) L-PRF increased the percentage of individuals who had a reduction in the wound area, but did not change the time for wound closure/healing up to 12 weeks of treatment. Conclusion: The set of results indicated that Leukocyte- and Platelet-Rich Fibrin favors the healing/repair process of chronic vascular lesions, anticipating healing. Because it is a low-cost treatment and potentially superior to conventional treatments, can reduce the pain of treated individuals with a consequent improvement in the quality of life of these patients.
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- 2022
65. Caracterización hematológica de hicoteas (Trachemys callirostris Gray, 1856) en Córdoba, Colombia
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Juan Carrascal Velásquez, Héctor Negrete Cartagena, César Rojano Bolaño, Gabriel Álvarez Otero, Julio Chacón Pacheco, and Juan Linares Arias
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eritrocitos ,hematología ,leucocitos ,Trachemys callirostris ,trombocitos ,Veterinary medicine ,SF600-1100 - Abstract
Se recolectaron 69 muestras de Trachemys callirostris en condiciones ex situ e in situ, para determinar sus parámetros hematológicos, comparar estos valores (considerando sexo, edad y condición) y establecer la morfología de sus células. A los animales se les extrajo sangre de la vena yugular. Se determinaron hematocrito, concentración de hemoglobina, recuentos total de eritrocitos, leucocitos y trombocitos e índices eritrocitarios (volumen corpuscular medio, hemoglobina corpuscular media y concentración de hemoglobina corpuscular media). En extendidos sanguíneos se realizaron el conteo diferencial de leucocitos y la descripción de la morfología de las células encontradas. Los valores promedio encontrados para todos los individuos fueron: hematocrito (29,9 % ± 5,1), hemoglobina (4,0 ± 1,0 g/dl), VCM (327 ± 15,3 fl), HCM (45 ± 17,5 pg), CMHC (14 ± 6 %), conteo total de trombocitos (15,0 ± 5,4 × 10³ μL), conteo total de eritrocitos (0,92 × 10⁶/μL ± 0,12), conteo total de leucocitos (12,10 ± 5,0 × 10³ μL). Al aplicar el análisis de varianza se encontró que no existe diferencia estadísticamente significativa (p < 0,05) entre Trachemys callirostris de distintos pesos (500 a 2000 g). Sin embargo, existen diferencias significativas (p < 0,05) y altamente significativas (p < 0,01) de la mayoría de las variables hematológicas entre sexos y entre hicoteas mantenidas en condición in situ y ex situ. De esta forma, las diferencias encontradas entre individuos, dependiendo su peso, sexo y condición de mantenimiento (in situ o ex situ), sirven como referencia para el establecimiento de protocolos de manejo de la especie en cautiverio.
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- 2014
66. Hematological Characterization of Hicotea Turtles (Trachemys callirostris [Gray, 1856]) in Córdoba (Colombia)
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Juan Carrascal Velásquez, Héctor Negrete Cartagena, César Rojano Bolaño, Gabriel Álvarez Otero, Julio Chacón Pacheco, and Juan Linares Arias
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eritrocitos ,hematología ,leucocitos ,Trachemys callirostris ,trombocitos. ,Veterinary medicine ,SF600-1100 - Abstract
69 samples from Trachemys callirostris were collected in situ and ex situ, to determine their hematological parameters, to compare these values (considering sex, age, and condition), and to establish the morphology of their cells. Blood was collected from the jugular vein of the animals. Hematocrit, hemoglobin concentration, total counts of erythrocytes, leukocytes and thrombocytes, and red cell indices were determined (mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration). Differential leukocyte count was performed, and the morphology of cells found in blood smears was described. The average values found for all individuals were: hematocrit (29.9% ± 5.1), hemoglobin (4.0 ± 1.0 g / dl), MCV (327 ± 15.3 fL), MCH (45 ± 17.5 pg), MCHC (14 ± 6%), total count of thrombocytes (15.0 ± 5.4 × 10³ μL), total count of erythrocytes (0.92 × 10⁶ / μL ± 0.12), and total count of leukocytes (12.10 ± 5.0 × 10³ μL). Analysis of variance showed no statistically significant difference (p < 0.05) between Trachemys callirostris of different weights (500 to 2000 g). Nevertheless, it showed significant (p < 0.05) and highly significant differences (p < 0.01) for most hematologic variables between sexes and hicotea turtles maintained in situ and ex situ. Thus, differences found between individuals, depending on weight, sex and condition of maintenance (in situ or ex situ), serve as a reference to establish protocols for maintaining turtles in captivity.
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- 2014
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67. El agregado de aceite de Sacha inchi disminuye la relación granulocitos/linfocitos postprandial producida por una comida rica en grasas, en adultos saludables
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Jose Guillermo Ortega, Isabella Echeverri, and Alicia Norma Alayón
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Nutrition and Dietetics ,Inflamación ,Periodo posprandial ,Leucocitos ,Ácidos grasos ,Dietas grasas ,Food Science - Abstract
RESUMEN La etapa postprandial se ha relacionado con cambios en marcadores inflamatorios, bioquímicos y celulares. El objetivo de este estudio fue evaluar los efectos postprandiales del agregado de aceite de Sacha inchi sobre la concentración y tamaño de leucocitos, eritrocitos y plaquetas, y en marcadores de inflamación, después de la ingesta de una comida rica en grasas. Una muestra de 42 individuos aparentemente sanos de sexo masculino consumió dos desayunos, uno de ellos adicionado con 15 mL de aceite rico en ácidos grasos poliinsaturados extraído de semillas de Sacha Inchi. Se tomaron muestras de sangre en ayunas y a las 4 horas postprandiales para determinar variables hematológicas (número y tamaño de leucocitos y plaquetas y distribución porcentual de leucocitos) y bioquímicas como interleukina 6 (IL6) y proteína C reactiva de alta sensibilidad (PCR-as). Con ambos desayunos aumentaron la concentración de PCR y los recuentos de leucocitos y plaquetas. El agregado de aceite de Sacha inchi aumentó el porcentaje de linfocitos (p= 0,005) y disminuyó el de granulocitos (p= 0,012), revirtiendo el aumento de la relación evidenciada luego de la ingesta grasa y las concentraciones de IL6. Los resultados permiten concluir que el agregado de 15 mL de aceite de Sacha inchi a un desayuno rico en grasas afecta la relación entre las diferentes poblaciones leucocitarias, lo que podría atenuar los efectos inflamatorios postprandiales y el riesgo cardiovascular. Registro: NCT02886169.
- Published
- 2021
68. Cellular response of the bovine mammary gland after Weissella confusa infusion to control Streptococcus agalactiae
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Liliana Serna-Cock, Cruz E Enríquez, Rómulo Campos Gaona, and Andrea Vásquez
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bacterias ácido lácticas ,leucocitos ,mastitis bovina ,Animal culture ,SF1-1100 - Abstract
Background: the use of lactic acid bacteria (LAB) as a potential therapeutic agent to control bovine mastitis was previously proposed. However, little is known about the cellular response of the bovine mammary gland in cattle infected with Streptococcus agalactiae and treated with LAB. Objective: to assess the cellular response by the mammary gland in lactating cows after infection with Streptococcus agalactiae followed by infusion with Weissella confusa as antibacterial treatment. Methods: healthy udder quarters of lactating cows were infected with S. agalactiae (10(7) cfu/mL). After 24 h of pathogen infusion, 50% of the quarters were infused with 109 cfu/mL of W. confusa (SW) and the remaining 50% were kept as control units (S). At days 1, 2, 3, 4, 5, 11, and 14 post-infusion of the pathogen, the clinical signs of mastitis and the degree of cellular response by the mammary gland were evaluated using the California mastitis test, somatic cell count, electrical conductivity, and differential leukocyte count in milk. Results: the SW quarters showed clinical inflammation of the mammary gland associated with a significant increase in somatic cell count, California mastitis test, electrical conductivity and high proportion of polymorphonuclear neutrophils. The results suggest that the infusion with W. confusa cells induced a higher cellular immune response in the bovine mammary gland than S. agalactiae alone. Conclusions: results indicate that W. confusa infusions for controlling S. agalactiae should not be adopted. However, the activation mechanism of somatic cells in the mammary gland needs to be elucidated.
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- 2013
69. Determinación de valores referenciales de reticulocitos, granulocitos (neutrófilos segmentados y en banda, eosinófilos y basófilos), monocitos, linfocitos, en equinos (Equus caballus) aparentemente sanos en condiciones de altitud
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Hernández Russi, Daniela and Masache Masache, Juan Leonardo
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RETICULOCITOS ,VETERINARIA ,LEUCOCITOS ,CABALLOS ,SANGRE - EXAMEN ,HEMATOLOGÍA VETERINARIA - Abstract
El presente trabajo experimental tuvo como objetivo determinar valores referenciales de reticulocitos y leucocitos en equinos (Equus caballus) aparentemente sanos en condiciones de altitud, en el Cantón Cuenca, provincia del Azuay. Para obtener valores reales de la zona y poder mejorar diagnósticos y tratamientos en el área de Medicina Veterinaria. The objective of this experimental work was to determine the reference values of reticulocytes and leukocytes in apparently healthy equines (Equus caballus) in altitude conditions, at the city of Cuenca, located in the province of Azuay. To obtain real values of the area and to be able to improve diagnoses and treatments in Veterinary Medicine.
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- 2022
70. Indicadores hematologicos y hormonales de estrés en aves de corral
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Franzini, Bárbara Domingues, Cruz, Lídia Caroline Ferreira, Sampaio, Stéfane Alves, Borges, Kelly Fernanda, Barros, Hemylla Sousa Santos, Santana, Fernanda Xavier de Castro, Gouveia, Alison Batista Vieira Silva, Paulo, Lorrayne Moraes de, and Minafra, Cibele Silva
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Aves de corral ,Thyroxine ,Erythrocytes ,Leucócitos ,Eritrocitos ,Eritrócitos ,Sistema endócrino ,Tiroxina ,Sistema endocrino ,Endocrine system ,Leukocytes ,Leucocitos ,Poltry ,Avicultura - Abstract
With the increasing advancement of poultry, problems with management and metabolic changes in birds began to appear on farms, especially heat stress. As it is a country with a tropical climate, temperature diversity is an important stressor to be considered by producers. In addition to thermal stress, several stressors deserve attention from producers, especially in the pre-slaughter management of birds. Capture techniques, improved transport and pre-slaughter waiting time planning are necessary for the best animal welfare, resulting in a better product offered to the consumer. When animals are subjected to adverse conditions, hormonal and hematological stimulation mechanisms are triggered with the intention of preparing and providing the necessary resources for the organism to face the compensatory emergency demands, aiming to maintain its balance. The objective of this literature review is to report the main changes that occur in hematological cells and plasma hormones during stress in poultry production, reporting the future perspectives of these analyses. Con el avance cada vez mayor de las aves de corral, comenzaron a aparecer en las granjas problemas con el manejo y los cambios metabólicos en las aves, especialmente el estrés por calor. Al ser un país con clima tropical, la diversidad de temperatura es un estresor importante a considerar por los productores. Además del estrés térmico, varios factores estresantes merecen la atención de los productores, especialmente en el manejo previo al sacrificio de las aves. Las técnicas de captura, el transporte mejorado y la planificación del tiempo de espera previo al sacrificio son necesarios para el mejor bienestar animal, lo que se traduce en un mejor producto ofrecido al consumidor. Cuando los animales son sometidos a condiciones adversas, se activan mecanismos de estimulación hormonal y hematológica con la intención de preparar y proporcionar los recursos necesarios para que el organismo enfrente las demandas compensatorias de la emergencia, visando mantener su equilibrio. El objetivo de esta revisión bibliográfica es reportar los principales cambios que ocurren en las células hematológicas y hormonas plasmáticas durante el estrés en la producción avícola, reportando las perspectivas futuras de estos análisis. Com o crescente avanço da avicultura, problemas com o manejo e alterações metabólicas nas aves, começaram a aparecer nas granjas, destacando-se o estresse calórico. Por se tratar de um país de clima tropical, a diversidade de temperatura mostra-se um importante fator estressante a ser considerado pelos produtores. Além do estresse térmico, vários fatores estressores merecem atenção dos produtores, principalmente no manejo pré-abate das aves. Técnicas de captura, melhoria no transporte e planejamento no tempo de espera pré-abate fazem-se necessárias para o melhor bem-estar animal, resultando em melhor produto ofertado ao consumidor. Quando os animais são submetidos a condições adversas, são desencadeados mecanismos de estímulo hormonal e hematológicos com a intenção de preparar e disponibilizar os recursos necessários para o organismo enfrentar as demandas emergenciais compensatórias, visando à manutenção do seu equilíbrio. Objetiva-se com esta revisão de literatura relatar as principais alterações que ocorrem nas células hematológicas e hormônios plasmáticos durante o estresse na produção avícola, relatando as perspectivas futuras dessas análises.
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- 2022
71. Treatment of endo-periodontal lesion using leukocyte- platelet- rich fibrin. A case report.
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Betancourt, Pablo, Elgueta, Ricardo, and Fuentes, Ramon
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LEUCOCYTES , *FIBRIN , *BLOOD platelets , *PERIODONTAL disease treatment , *BICUSPIDS , *BONE resorption , *CLINICAL medicine , *COMPUTED tomography , *ENDODONTICS , *DENTAL implants , *EVALUATION of medical care , *PALATE , *PERIODONTAL disease , *PERIODONTITIS , *POSTOPERATIVE period , *OPERATIVE surgery , *DENTAL extraction , *TOOTH roots , *THREE-dimensional imaging , *TREATMENT effectiveness - Abstract
Case Description: The main objective of this paper was to report the clinical effectiveness of leukocyte- platelet-rich fibrin (L-PRF) in the treatment of a combined endo-periodontal lesion of an upper first premolar. Clinical Findings: The tooth had a profound abfraction on the vestibular aspect and presented no mobility but revealed a deep pocket measuring of 11 mm on the mesial vestibular aspect and 14 mm on the mesial palatine aspect. The three dimensional image analysis showed total bone loss in the mesial aspect and an extensively bone loss of the vestibular aspect of the vestibular root. Treatment and Outcome: Endodontic treatment was performed and periodontal access surgery (surgical periodontal therapy) was done with the application of autologous L-PRF. Three month and 6 months after surgery, the cone beam computed tomography (CBCT) exams showed no bone regeneration in any aspect of the tooth. However, periodontal examination showed a significative improvement in the deepness of surcus. The mesial vestibular aspect had a deep pocket of 3 mm and 5 mm on the mesial palatine aspect showing a reduction in deepness of 8 mm and 9 mm, respectively. Clinical Relevance: The actual treatment for teeth with bad prognosis is the extraction and replacement with implants. Even though implants are capable of restore function and aesthetic, the abuse of this approach have led to the loss of teeth that could be successfully treated with a less invasive technique. The prognosis of teeth with endoperiodontal lesion is poor but could be enhanced with regenerative therapies. Until now there are no clinical trials and just four case report about the treatment of these teeth with platelet rich fibrin. [ABSTRACT FROM AUTHOR]
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- 2017
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72. Hematimetria manual e automática em jaguatiricas (Leopardus pardalis - Linnaeus, 1758).
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Silva, T. D. P., Lacerda, L. A., Carvalho, L. S., Souza, S. N., Arnhold, E., Sant'Ana, F. J. F., and Fioravanti, M. C. S.
- Abstract
Copyright of Arquivo Brasileiro de Medicina Veterinaria e Zootecnia is the property of Universidade Federal de Minas Gerais, Escola de Veterinaria and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2017
- Full Text
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73. Detección de Neospora caninum por PCR anidada en leucocitos de bovinos productores de leche.
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Miguel Reyes-Sandoval, Raúl, Antonio Álvarez-Martínez, Jesús, Rojas-Martínez, Carmen, Espinosa-Ayala, Enrique, Guadalupe García-Rubio, Virginia, and José Ojeda-Carrasco, Juan
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- 2017
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74. Perfil hematológico del guajolote criollo de traspatio en la región de Tecamachalco, Puebla.
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Reséndiz Martínez, Roberto, Romero Castañón, Salvador, Jiménez Cortez, Herminio I., and Castro Bautista, Briseida Lucía
- Abstract
Copyright of Revista Biológico Agropecuaria Tuxpan is the property of Revista Biologico Agropecuaria Tuxpan and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2017
75. Nuevas tendencias en regeneración tisular: fibrina rica en plaquetas y leucocitos.
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Salgado-Peralvo, Ángel Orión, Salgado-García, Ángel, and Arriba-Fuente, Lorenzo
- Abstract
Copyright of Revista Española de Cirugía Oral y Maxilofacial is the property of Sociedad Espanola de Cirugia Oral y Maxilofacial (SECOM) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2017
- Full Text
- View/download PDF
76. Comparative hematological analysis of Morada Nova and Santa Inês ewes in all reproductive stages.
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Bezerra, Leílson R., Oliveira, Wagner D. C., Silva, Tairon P. D., Torreão, Jacira N. C., Marques, Carlo A. T., Araújo, Marcos J., and Oliveira, Ronaldo L.
- Abstract
Copyright of Pesquisa Veterinaria Brasileira is the property of Colegio Brasileiro de Patologia Animal - CBPA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2017
- Full Text
- View/download PDF
77. Aspectos morfológicos e ultraestruturais de células sanguíneas de Crotalus durissus terrificus.
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Kindlovits, Lívia M., Miranda, Farlen J. B., Damasceno-Sá, João Claudio, Da Matta, Renato A., and Almosny, Nádia R. P.
- Abstract
Copyright of Pesquisa Veterinaria Brasileira is the property of Colegio Brasileiro de Patologia Animal - CBPA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2017
- Full Text
- View/download PDF
78. PARÁMETROS INMUNOLÓGICOS EN OVINOS DE PELO PARA ABASTO CONTRA NEMATODOS GASTROINTESTINALES EN TABASCO, MÉXICO.
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Alvarado-Alvarado, A., González-Garduño, R., Zaragoza-Vera, M., Zaragoza-Vera, C., Arjona-Jiménez, G., López-Arellano, M. E., and Navarro Martinez, F.
- Abstract
Copyright of Agro Productividad is the property of Colegio de Postgraduados and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2017
79. Factores asociados con la conversión de la colecistectomía laparoscópica a colecistectomía abierta.
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Ledmar Vargas, R., Milena Agudelo, S., Roger Lizcano, C., Melissa Martínez, B., Leonardo Velandia, B., Sergio Sánchez, H., Óscar Jiménez, P., and Quintero, Marco
- Abstract
Acute cholecystitis is an inflammation of the vesicular wall whose treatment is purely surgical: a laparoscopic cholecystectomy is the procedure of choice. This can be converted intraoperatively due to complications of the intervention, patient factors or factors associated with the surgeon. The aim of this study was to establish the frequency and the association of conversions of laparoscopic cholecystectomies based on hematological laboratory tests and abdominal ultrasound as well as sociodemographic factors. Materials and Methods: This is a descriptive, cross-sectional, observational and retrospective study that was carried out from January 1 to November 3, 2015. Clinical histories of patients who had been with diagnosed with benign vesicular pathologies who underwent vesicular surgical procedures were reviewed. Results: Of the cases reviewed, 35.5% patients underwent laparoscopic cholecystectomies, and of these cases 42.8% of the procedures were converted to conventional surgery. The majority of the patients were women (72.8%), but conversions were most frequent among men. The most frequent preoperative diagnosis was cholelithiasis (98.3%). Age greater than 50 years presented an odds ratio of 0.55, while leukocytosis had an odds ratio of 0.40, both variables were statistically significant (P = <0.05). Conclusions: It was determined that ages over 50 years and/or a leukocyte count over 10,000 mm3 are risk factors for failure of laparoscopic cholecystectomies. In addition, factors related to the surgeon must be taken into account. [ABSTRACT FROM AUTHOR]
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- 2017
- Full Text
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80. Effects of intensified training and taper on immune function
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Elena Papacosta and Michael Gleeson
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Exercício ,Treinamento ,"Tapering ,Imunidade ,Leucócitos ,Imunoglobulinas ,Atletas ,Exercise training ,Tapering ,Immunity ,Leukocytes ,Immunoglobulins ,Athletes ,Sports ,GV557-1198.995 - Abstract
Although resting immune function is not very different in athletes compared with non-athletes periods of intensified training (overreaching) in already well trained athletes can result in a depression of immunity in the resting state. Illness-prone athletes appear to have an altered cytokine response to antigen stimulation and exercise. Having low levels of salivary IgA secretion also makes athletes more susceptible to upper respiratory tract infections. Overtraining is associated with recurrent infections and immunodepression is common, but immune functions do not seem to be reliable markers of impending overtraining. There are several possible causes of the diminution of immune function associated with periods of heavy training. One mechanism may simply be the cumulative effects of repeated bouts of intense exercise (with or without tissue damage) with the consequent elevation of stress hormones, particularly glucocorticoids such as cortisol, causing temporary inhibition of TH-1 cytokines with a relative dampening of the cell-mediated response. When exercise is repeated frequently there may not be sufficient time for the immune system to recover fully. Tapering has been described as a gradual reduction in the training load which allows the recovery of physiological capacities that were impaired by previous intensive training and permits further training-induced adaptations to occur accompanied by competition performance enhancements. The majority of the studies that have examined the recovery of immunoendocrine responses during 1-3 week tapers in trained athletes have mainly reported enhanced performance, often accompanied by increased anabolic activity, reduced physiological stress and restoration of mucosal immunity and immune function.Quando se compara a função imune, em repouso, de atletas e não atletas, não se verificam grandes diferenças. Porém, períodos de treinamento intensificado ("overreaching") em atletas bem treinados podem induzir supressão da imunidade no estado de repouso. Os atletas com maior propensão para contrair doenças parecem apresentar uma resposta alterada de citocinas, tanto quando estas são estimuladas por antígenos, quanto em resposta ao exercício propriamente dito. Baixos níveis de secreção de IgA salivar também contribuem para tornar os atletas mais susceptíveis à infecções do trato respiratório superior. A síndrome do "overtraining" é associada a infecções recorrentes e a imunossupressão é comum; no entanto, marcadores da função imune não parecem ser suficientemente sensíveis ao "overtraining" eminente. Existem várias possíveis causas para a diminuição da função imune associadas com períodos de treinamento severo. Um possível mecanismo pode ser simplesmente, o efeito acumulativo de atividades e sessões repetidas de exercício intenso (com ou sem dano tecidual), com a consequente elevação dos hormônios de estresse, particularmente os glicocorticóides como o cortisol, causando assim, uma inibição temporária das citocinas de TH-1, com uma relativa atenuação da resposta imune celular. Quando o exercício é repetido frequentemente, pode não haver tempo suficiente para uma total recuperação do sistema imunológico. O "Tapering" tem sido descrito como uma gradual redução na carga de treinamento a qual permite a recuperação das capacidades fisiológicas, que por sua vez, foram afetadas pelo treinamento intensivo anterior, permitindo assim, que adaptações adicionais decorrentes do treinamento ocorram, acompanhadas pelo incremento do desempenho competitivo. A maioria dos estudos que investigaram a recuperação das respostas imuno-endócrinas em atletas durante uma a três semanas de "taper" tem registrado aumento do desempenho, frequentemente acompanhado pelo aumento da atividade anabólica, redução do estresse fisiológico e restabelecimento da imunidade das mucosas e da função imune.
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- 2013
81. Efeito do treinamento físico moderado e intenso sobre os mecanismos de defesa de ratos adultos Effect of moderate and intense physical training on the mechanisms of defense in adult rats
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Glívia Maria Barros Delmondes, Danielly Cantarelli de Oliveira, Patrícia Clara Pereira dos Santos, Marcelo Tavares Viana, Maria do Socorro Brasileiro Santos, Célia Maria Machado Barbosa de Castro, and Maria Amparo Andrade
- Subjects
Exercício físico ,Leucócitos ,Fagocitose ,Óxido nítrico ,Physical exercise ,Leukocytes ,Phagocytes ,Nitric oxide ,Sports ,GV557-1198.995 ,Sports medicine ,RC1200-1245 - Abstract
OBJETIVO: Analisou-se o impacto do treinamento físico moderado (TFM) e intenso (TFI) sobre o perfil leucocitário e a atividade microbicida de macrófagos alveolares, em 29 ratos machos Wistar. MÉTODOS: Foram formados três grupos: controle-sedentário (CS), treino-moderado (TM) e treino-intenso (TI). Os TFM e TFI foram efetuados através da natação, com aumento progressivo de carga conforme o peso corporal, até um máximo de 3% para o TFM, e 5% para o TFI. As coletas de sangue para contagem total e diferencial dos leucócitos foram automatizadas através do analisador hematológico Sysmex XT- 1800i (Roche®) antes e após o treino. E ao final realizou-se o lavado broncoalveolar para determinar a taxa de fagocitose e a produção de óxido nítrico (ON) de macrófagos. RESULTADOS: O grupo TM apresentou valores maiores para o número de leucócitos (12,77±2,0 x 17,25±2,4 10³/mm³), linfócitos (8,87±1,0 x 12,5±2,1 10³/mm³) e neutrófilos (0,99±0,5 x 3,18±1,0 10³/mm³), pOBJECTIVE: To assess the impact of moderate (MPT) and intense physical training (IPT) on the white blood cell profile and the microbicide activity of alveolar macrophages in 29 Wistar male rats. METHODS: The sample was separated into three groups: control group (sedentary), moderate training (MT), and intense training groups (IT). Swimming was the tool used to classify both moderate and intense training groups with a progressive increase of exercise load regarding body weight (up to 3% for the moderate physical training and 5% for the intense training group). The blood samples used to count total and differential leucocytes were automated by a hematological analyzer Sysmex XT-1800i (Roche®) before and after training sessions. At the end of the process, a bronchoalveolar lavage was carried out so to determine the amount of phagocytosis and the production of nitric oxide (NO) of the macrophages. RESULTS: The MT showed an increase in the number of leucocytes (12,77±2,0x17,25±2,4 10³/mm³), lymphocytes (8,87±1,0x 12,5±2,1 10³/mm³) and neutrophils (0,99±0,5x3,18 ±1,0 10³/mm³), p
- Published
- 2012
- Full Text
- View/download PDF
82. Descripción de líneas celulares sanguíneas de pacientes con blastos provenientes de cinco ciudades de Colombia. Serie de casos
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Adriana Lancheros, Mabely Juliana Mosquera-Heredia, Yusselfy Márquez-Benítez, Shirley Gigiola Cruz-Rubio, Julieth Esteher Oliveros-Barros, and Ciencia UDES
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Pediatrics ,medicine.medical_specialty ,Anemia ,Células sanguínea ,Population ,Throm-bocytopenia ,Neutropenia ,03 medical and health sciences ,0302 clinical medicine ,Leukocytes ,medicine ,Leucemia ,Leucocitos ,Leukocytosis ,education ,education.field_of_study ,Blood Cells ,Leukemia ,business.industry ,Public health ,General Medicine ,medicine.disease ,Trombocitopenia ,Thrombocytopenia ,Células Sanguíneas ,030220 oncology & carcinogenesis ,Hypochromia ,Etiology ,medicine.symptom ,business ,030215 immunology - Abstract
Digital, Introducción: las leucemias constituyen un grupo complejo de hemopatías que por su etio-logía, patogenia y mortalidad, son consideradas un problema de salud pública. Su detección temprana permite la remisión oportuna para la confirmación del diagnóstico y el inicio del tratamiento.Objetivo: describir la celularidad sanguínea de pacientes con presencia de blastos en sangre periférica, provenientes de cinco ciudades de Colombia durante el año 2017.Métodos: estudio tipo descriptivo de serie de casos. A partir de hemogramas y sus respectivos extendidos de sangre periférica, se analizó cualitativa y cuantitativamente las células sanguí-neas de los pacientes con blastos que cumplieron con los criterios de inclusión.Resultados: fueron incluidos 39 pacientes con presencia de blastos de novo en sangre peri-férica procedentes de Cúcuta 48,7 %, Valledupar 33,3 %, Tunja 10,3 %, Socorro 5,1 % y Villavi-cencio 2,6 %. El 100 % eran afiliados al sistema general de seguridad social en salud en donde predominó el sexo femenino con el 54 %, la edad promedio para las mujeres fue de 32,5 años y para los hombres fue de 44,2 años. El 76 % de los pacientes presentó leucocitosis de moderada a grave y 69,2 % neutropenia. El 95 % presentó anemia, 51 % hipocromía y 38 % microcitos. El 74 % presentó trombocitopenia y 15 % macroplaquetas.Conclusiones: los hallazgos concuerdan con lo reportado por la literatura en relación con el predominio de la enfermedad de acuerdo con la edad y el sexo. La anemia, trombocitopenia y neutropenia, se mantienen como característica constante en los pacientes con leucemia.INVESTIGACIÓN ORIGINAL, Introduction: Leukemia constitutes a complex group of haemopathies, which due to their etiology, patho-genesis and mortality, can be considered a public health problem. Its early detection allows for timely remission for confirmation of the diagnosis and early start of treatment.Objective: To describe the sanguineous cellularity in a population with presence of blasts in peripheral blood coming from different regions of Colombia du-ring the year 2017. Methods: A descriptive study, type case series. He-mograms and peripheral blood smears from patients with blast presence or a presumptive diagnosis of leu-kemia were analyzed in five cities of Colombia, all of whom met the inclusion criteria. Results: 39 patients with presence of de novo blasts from Cúcuta 48.7%, Valledupar 33.3%, Tunja 10.3%, So-corro 5.1% and Villavicencio 2.6% were found. All the individuals were affiliated to the Colombian health-care system. Fifty four percent of the subjects were female, with an average age of 44.2 years for men and 32.5 for women. Seventy six percent of the pa-tients presented moderate to severe leukocytosis and 69.2% neutropenia. Ninety-five percent had anemia, 51% hypochromia and 38% microcytes, 74% presented thrombocytopenia and 15% macroplates. Conclusions: These findings are a match to those found in the literature, as for the predominance of the disease by age and sex. Anemia, thrombocytopenia and neutropenia are a set of characteristics that re-main constant in patients with leukemia., Ciencias Médicas y de la Salud
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- 2020
83. The dermatophyte trichophyton rubrum induces neutrophil extracellular traps release by human neutrophils
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Ana Paula Carvalho Reis, Giovanna Azevedo Celestrino, Mariana Villas Bôas Igoa, Thais Martins Jesus, Tábata Takahashi França, Daniel Valério Silva Moreira, Paula Ordonhez Rigato, Paula Keiko Sato, Antonio Condino-Neto, Irene L. Noronha, Luciane Alarcão Dias-Melicio, Pritesh Jaychand Lalwani, Gil Benard, Maria Gloria Teixeira Sousa, Universidade de São Paulo (USP), Instituto Adolfo Lutz, Universidade Estadual Paulista (UNESP), and Fiocruz Amazônia
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Innate immunity ,Microbiology (medical) ,Neutrophils ,Trichophyton rubrum ,Plant Science ,bacterial infections and mycoses ,Neutrophil extracellular traps ,LEUCÓCITOS ,dermatophytosis ,neutrophils ,neutrophil extracellular traps ,fungal killing ,innate immunity ,Dermatophytosis ,Fungal killing ,bacteria ,skin and connective tissue diseases ,Ecology, Evolution, Behavior and Systematics - Abstract
Made available in DSpace on 2022-04-29T08:39:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-02-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Neutrophils are the first leukocytes recruited to the site of infection and are thought to be responsible for fungal elimination from the skin such as dermatophytes. Neutrophils are able to secrete reactive oxygen species (ROS) and neutrophil extracellular traps (NETs) that can kill different fungi, including Aspergillus, spp., Candida albicans, and Phialophora verrucosa. However, NET production in response to Trichophyton rubrum, the main etiologic agent of dermatophytosis, has yet to be studied. We demonstrated that human neutrophils produce NETs against different morphotypes of T. rubrum in a dose-dependent manner and NET formation is dependent on ROS production. In addition, ROS production by human neutrophils in response to T. rubrum is dependent on NADPH oxidase, but not on fungal viability. NETs mediated killing of T. rubrum. Collectively, these results demonstrate that T. rubrum was able to trigger the production of NETs, suggesting that these extracellular structures may represent an important innate immune effector mechanism controlling physiological response to T. rubrum infection. Laboratory of Medical Mycology LIM-53 Clinical Dermatology Division Hospital das Clínicas FMUSP Faculdade de Medicina FMUSP Institute of Tropical Medicine University of São Paulo Laboratory of Cellular Genetic and Molecular Nephrology Division of Nephrology University of São Paulo School of Medicine Department of Immunology Institute of Biomedical Sciences University of São Paulo Centro de Imunologia Instituto Adolfo Lutz Laboratory of Medical Investigation in Immunology (LIM-48) Hospital das Clinicas HCFMUSP Faculdade de Medicina Universidade de Sao Paulo Department of Pathology Botucatu Medical School UNESP-São Paulo State University Leonidas e Maria Deane Institute Fiocruz Amazônia Department of Pathology Botucatu Medical School UNESP-São Paulo State University FAPESP: 2016/16369-1 FAPESP: 2017/26208-8 FAPESP: 2018/16146-8 FAPESP: 2018/19191-2 FAPESP: 2018/24175-8
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- 2022
84. Estudio del efecto de la cirugía de bypass gástrico en Y de Roux sobre los mecanismos moleculares asociados a la fisiopatología de la obesidad
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Abad Jiménez, Zaida María, Rocha Barajas, Milagros, Víctor González, Víctor Manuel, and Departament de Fisiologia
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mitocondrias ,interacción leucocito-endotelio ,metformina ,leucocitos ,estrés oxidativo ,inflamación ,UNESCO::CIENCIAS MÉDICAS ,tejido adiposo ,obesidad ,autofagia - Abstract
La obesidad es una enfermedad multifactorial e incapacitante que se caracteriza por una sobrecarga metabólica del tejido adiposo (AT), la inflamación crónica de bajo grado, el estrés oxidativo y la disfunción endotelial. En la actualidad, la prevalencia mundial de la obesidad está aumentando, donde el estilo de vida influye de manera significativa en la resolución de esta enfermedad. En este contexto, ha aumentado la demanda de estrategias para revertir las comorbilidades y frenar su aumento. El procedimiento de bypass gástrico en Y de Roux (RYGB) es una herramienta eficaz para la pérdida de peso y la remisión de las comorbilidades metabólicas de la obesidad, aunque se desconocen los mecanismos moleculares que implican estas mejoras. Por lo tanto, el objetivo principal de la presente tesis doctoral fue determinar cómo la cirugía RYGB modula la inflamación crónica de bajo grado, el estrés oxidativo y la disfunción endotelial, explorando su efecto sobre el complejo del inflamasoma, la autofagia, la dinámica mitocondrial y el estrés del retículo endoplásmico (ER). También se abordó el papel de la metformina, un enfoque terapéutico alternativo para la obesidad y la diabetes de tipo 2 (T2D). Por lo tanto, otro objetivo de este estudio fue analizar los mecanismos moleculares relacionados con la inflamación y la autofagia en la AT y el efecto protector de la metformina contra las alteraciones metabólicas. Nuestra hipótesis fue que la pérdida de peso inducida por la RYGB modula la respuesta de los leucocitos, componentes primordiales del sistema inmunitario. Para comprobar esta hipótesis, analizamos las distintas vías metabólicas que se ven alteradas por la obesidad y exploramos si el AT responde de forma diferencial a la metformina. Observamos que la pérdida de peso producida tras 1 año de RYGB se acompañaba de una mejora del perfil lipídico proaterogénico y de la sensibilidad a la insulina. Esto se acompañó de una disminución de las citocinas proinflamatorias y de la producción de especies reactivas de oxígeno de los leucocitos y de una mayor respuesta antioxidante, lo que dio lugar a una menor interacción entre los leucocitos y el endotelio y a marcadores de disfunción endotelial. Además, un análisis más detallado de los mecanismos moleculares en los leucocitos tras la pérdida de peso reveló una mejora de la respuesta homeostática como resultado de un aumento de la AMPK, de los marcadores de autofagia, de la biogénesis y de la dinámica mitocondrial, junto con una disminución de las citoquinas proinflamatorias, de los marcadores de inflamación y del estrés del ER. En conjunto, estos resultados ayudan a arrojar luz sobre los mecanismos que subyacen al efecto protector de la pérdida de peso en el control metabólico y la homeostasis celular en la obesidad. Además, mediante un estudio transversal, observamos que los pacientes con obesidad y T2D tratados con metformina presentan una mejora del perfil inflamatorio y oxidativo con respecto a los sujetos con obesidad metabólicamente sana (MHO). Esta mejora parece estar modulada por cambios en la activación del complejo del inflamasoma y la autofagia en el AT visceral, lo que sugiere -a pesar de la creencia común- que los sujetos MHO no están tan protegidos cardiometabólicamente como se espera. Por lo tanto, se sugiere que estudios futuros presenten como objetivo determinar las dianas directas de la metformina responsables de mediar en estas respuestas. Obesity is a multifactorial and disabling disease characterized by metabolic overload in adipose tissue (AT), chronic low-grade inflammation, oxidative stress and endothelial dysfunction. Currently, the worldwide prevalence of obesity is increasing, with lifestyle having a significant influence on the resolution of this disease. In this context, there has been an increase in the demand for strategies to reverse comorbidities and to stop its increase. The Roux-en-Y gastric bypass (RYGB) procedure is an effective tool for weight loss and remission of the metabolic comorbidities of obesity, although the molecular mechanisms involving these improvements are unknown. Therefore, the main objective of the present PhD thesis was to determine how RYGB surgery modulates chronic low-grade inflammation, oxidative stress and endothelial dysfunction, by exploring its effect on the inflammasome complex, autophagy, mitochondrial dynamics and endoplasmic reticulum (ER) stress. The role of metformin, an alternative therapeutic approach to obesity and type 2 diabetes (T2D), was also addressed. Therefore, another aim of this study was to analyse the molecular mechanisms related to inflammation and autophagy in AT and the protective effect of metformin against metabolic alterations. Our hypothesis was that RYGB-induced weight loss modulates the response of leukocytes, primordial components of the immune system. To test this hypothesis, we analysed the various metabolic pathways that are altered by obesity and explored whether AT responds differentially to metformin. We observed that the weight loss produced after 1 year of RYGB was accompanied by an improvement in proatherogenic lipid profile and insulin sensitivity. This was accompanied by a decrease in proinflammatory cytokines and leukocyte reactive oxygen species production and an enhanced antioxidant response, resulting in less interaction between leukocytes and both the endothelium and markers of endothelial dysfunction. In addition, further analysis of molecular mechanisms in leukocytes after weight loss revealed an improvement in the homeostatic response as a result of an increase in AMPK, markers of autophagy, biogenesis and mitochondrial dynamics, along with a decrease in proinflammatory cytokines, markers of inflammation and ER stress. Considered together, these results help to shed light on the mechanisms underlying the protective effect of weight loss on metabolic control and cellular homeostasis in obesity. Moreover, through a cross-sectional study, we reported that obese T2D patients treated with metformin exhibit improved inflammatory and oxidative profile with respect to metabolically healthy obesity (MHO) subjects. This improvement seems to be modulated by changes in the activation of the inflammasome complex and autophagy in VAT, suggesting – despite common belief – that MHO subjects are not as cardiometabolically protected as expected. Future mechanistic studies should aim to determine the direct targets of metformin responsible for mediating these responses.
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- 2022
85. Molecular determinants of the Kv1.3/KCNE4 interaction
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Sastre Martinez, Daniel, Felipe Campo, Antonio, and Universitat de Barcelona. Departament de Bioquímica i Biomedicina Molecular
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Pharmacology ,Farmacologia ,Canals iònics ,Leucòcits ,Farmacología ,Immunology ,Inmunología ,Ciències Experimentals i Matemàtiques ,Canales iónicos ,Ion channels ,Leucocitos ,Immunologia ,Leucocytes - Abstract
[eng] Kv1.3 plays a crucial role in leukocytes, where it regulates activation and proliferation. In these cells, Kv1.3 is coexpressed with modulatory subunit KCNE4. This ancillary peptide acts as a dominant negative regulator of the channel with a dual function. On the one hand, KCNE4 triggers the intracellular retention of Kv1.3 reducing K+ currents. On the other, KCNE4 enhances their C- type inactivation kinetics. Thus, the aim of this thesis was to characterize the molecular mechanisms of Kv1.3/KCNE4 association and modulation in the context of the immune system physiology. To do so, the thesis is structured in three chapters. Chapter I (KCNE4 in the immune system). We confirmed the physiological role of KCNE4 in immune cells like T lymphocytes and dendritic cells (Contribution 1). By manipulating the expression of KCNE4 in these cells, we were the first to describe a relationship between KCNE4 and the leukocyte function. Our results highlight a possible therapeutic role for KCNE4. Chapter II (A protein interaction hub (L69-72) in the C-terminus of KCNE4). We analysed the relationship between Kv1.3, KCNE4 and calmodulin (CaM). We identified a tetraleucine motif in the C-terminus of KCNE4 (L69-72) that determines association to Kv1.3 (Contribution 2). In a nearby position, an ER retention motif (ERRM) triggers intracellular retention of the channel. These results were replicated in a peptide containing just 22 residues of KCNE4, including both clusters. This peptide partially replicated the inhibitory effects of KCNE4 and thus, a potential pharmacological interest. We are also the first to propose a structural model for the Kv1.3/KCNE4 association. On the other hand, L69-72 in KCNE4 also determines CaM binding. The role of Ca2+/CaM is relevant in the context of the immune system physiology, where Kv1.3 potentiates the calcium signalling. Furthermore, we are the first to report dimerization of KCNE4 (Contribution 3), which modulates the traffic of KCNE4. Dimerization was also mediated by the leucine cluster, acting as a protein interaction hub. We propose CaM binding as a mechanism to fine-tune interactions with Kv1.3 and dimerization. Chapter III (Structural relationships of the Kv1.3/KCNE4 complex). We focused on the structural Kv1.3/KCNE4 association. A series of chimaeras between KCNE2 and KCNE4 allowed us to decipher roles for each domain of KCNE4 (Contribution 4). Thus, we confirmed that the C- terminus of KCNE4 mediates association and intracellular retention of Kv1.3. Moreover, for the first time we characterized the molecular determinants of KCNE4-dependent kinetic modulation of Kv1.3. These results were confirmed with Gibbs free energy calculations and the Kv1.3/KCNE4 structural model. Using alanine-scanning mutagenesis, we mapped some residues implicated in this process. Moreover, we found that, similar to other Kv/KCNE associations, Kv1.3/KCNE4 associated in an open stoichiometry with up to four KCNE4 subunits per complex (Contribution 5). The number of KCNE4 peptides in the channelosome was proportional to the effects on activation kinetics and current amplitude. However, the accentuation of Kv1.3 inactivation was independent of stoichiometry. The similarities in stoichiometry and docking to Kv channels suggest a conserved mechanism among KCNE peptides. Finally, because KCNE4 caused structural Kv1.3 changes, we studied the effect of KCNE4 on Kv1.3 pharmacology (Contribution 6). We studied margatoxin and Psora-4, two well-characterized Kv1.3 inhibitors with different binding sites. Our results point out the relevance of considering auxiliary subunits in the development of therapeutic approaches. In conclusion, this thesis deepens the knowledge of the molecular mechanisms of Kv1.3 modulation by KCNE4. We provide a structural insight into Kv channel modulation while considering the physiological context. Our results are of considerably physiological interest due to the multitherapeutic potential of Kv1.3.
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- 2022
86. Análise de diferentes processamentos da fibrina rica em plaquetas
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Júlia Zamin DALLOSTO, Matheus Albino SOUZA, Luciane Dellazari da Silva do PRADO, and Luciano de Oliveira SIQUEIRA
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Processamento ,procedimentos odontológicos ,blood concentrate ,leukocytes ,dental procedures ,leucócitos ,General Medicine ,amostra ,Processing ,sample ,concentrado sanguíneo - Abstract
Introduction Platelet-rich fibrin (PRF) is formed by an autologous blood concentrate, with properties that promote cell proliferation and regenerationof bone, gingival and epithelial tissue. Objective To compare four different procedures for processing as well as obtaining PRF, and analyzing their formation through laboratory techniques. The purpose of the study is to validate a method that produces higher quality PRF for oral surgery use in different branches of dentistry. Material and method The experiment consisted of collecting blood from 12 volunteers, and processing each patient’s sample in 4 different ways. In the following two-stage process analysis, the first, quantitative, step was to analyze the samples' platelet-poor plasma (PPP),with a Neubauer Hemocytometer to count blood components. In the second, qualitative step, the sample’s PRF were analyzed under microscopy using histological slides. Result The statistical analysis of the leukocyte, red blood cell and platelet count did not show any significant distinction when comparing different processes. Histological analysis of the PRF showed fibrin network with platelet aggregation, several leukocytes and presence of red blood cells, with double centrifuged samples presenting more white blood cells than the others. Conclusion: Among the analyzed procedures, the sample centrifuged once at 3000 RPM (1900 G) for 10 min showed the best quality PRF. Resumo Introdução A fibrina rica em plaquetas (FRP) é formada por um concentrado sanguíneo autólogo, com propriedades de promover a proliferação celular, regeneração de tecido, dentre eles tecido ósseo, gengival e epitelial. Objetivo Comparar quatro tipos diferentes de processamento e obtenção de FRP, analisando a sua formação através de técnicas laboratoriais. A finalidade do estudo é validar um método que obtenha maior qualidade para o uso em cirurgias nas diversas áreas da odontologia. Material e método O experimento foi constituído de uma coleta de sangue de 12 voluntários, onde a amostra de cada paciente foi processada de 4 formas. A análise dos processamentos se deu em duas etapas, onde na primeira, quantitativa, foi analisado o plasma pobre em plaquetas (PPP), através de contagem de constituintes sanguíneos em câmara de Neubauer. Na segunda etapa, qualitativa, foram analisadas as FRP em microscopia através de lâminas histológicas. Resultado A análise estatística da contagem de leucócitos, hemácias e plaquetas não mostraram diferença significativa entre os distintos processamentos. As análises histológicas do FRP mostraram redes de fibrina com agregação plaquetária, inúmeros leucócitos e presença de hemácias, sendo que as amostras de dupla centrifugação apresentaram mais leucócitos que nas demais. Conclusão Dos procedimentos analisados, a amostra centrifugada a 1 única vez a 3000 RPM (1900 G) por 10 min mostrou-se a melhor qualidade de FRP.
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- 2022
87. Influence of Sexual Dimorphism, Aging, and Differential Cell Capture Efficiency of Blood Separation Systems on the Quality of Platelet-Rich Plasma
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Bibiana Trevissón, Ricardo Becerro-de-Bengoa-Vallejo, David Sevillano, Natalia González, Marta Elena Losa-Iglesias, Daniel López-López, and Luis Alou
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Aging ,Envejecimiento ,General Medicine ,Blood separation–concentration system ,Sexual dimorphism ,Plasma rico en plaquetas ,Platelet-rich plasma ,platelet-rich plasma ,leukocytes ,sexual dimorphism ,aging ,blood separation–concentration system ,Psicología sexual ,Leukocytes ,Hematología ,Leucocitos ,Dimorfismo sexual - Abstract
Few studies have checked the impact of the hormonal/immunological dimorphism of patients on the cellular composition of platelet-rich plasma products (PRP). Whole blood (WB) from 26 volunteers was concentrated using a device previously characterized. Platelet and white blood cell (WBC) counts in WB and PRP were compared between genders, and after the population was split into pre (≤50 years)- and post (>50 years)-menopausal ages. In WB, platelet–total WBC densities were comparable in men and women. The phagocytic cell composition differed (p ≤ 0.04). After dividing by ages, phagocytic cell discrepancies were linked to women > 50 years (p ≤ 0.05), and differences emerged in lymphocyte counts (lower in >50 years groups, within and between genders, p ≤ 0.05). In PRP, densities were significantly higher, but the PRP/WB ratios varied according to blood cell (lower for phagocytic cells) and between subjects (more favorable at a lower density of a particular blood cell in WB). This “system compensatory efficiency” reduced/reverted PRP differences in the leukocyte composition between genders/age–sex groups in WB. In PRP, neutrophils were higher in younger men than older women (p < 0.05). WB lymphocyte differences between age–sex groups persisted. Age is a more determining factor than sex in the preparation of PRP. Post-menopause, sexual dimorphism strongly influences the composition of leukocytes, also conditioned by the capture efficiency of the system.
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- 2022
88. WBC count and functional changes induced by co-administration of clofazimine and clarithromycin, in single and multiple doses, in Wistar rats
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Flávia Aparecida Paina, Julise Cunha Miranda, Cristiane Fernandes de Freitas Tavares, Regina Helena da Costa Queiroz, and Ana Maria de Souza
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Clofazimina ,Claritromicina ,Hanseníase ,Mycobacterium avium ,Leucócitos ,Fagocitose ,Burst oxidativo ,Clofazimine ,Clarithromycin ,Leprosy ,Leukocytes ,Phagocytosis ,Oxidative burst ,Pharmacy and materia medica ,RS1-441 - Abstract
Clofazimine and clarithromycin are used to treat leprosy and infections caused by Mycobacterium avium complex. Little data on the toxicity of co-administration of these two drugs are available. Here we evaluated the potential adverse effects of polytherapy with these two drugs in male Wistar rats by determining WBCs counts and other blood cell counts, neutrophilic phagocytosis, and burst oxidative, by flow cytometry. We observed an increase in WBCs, in multiple-dose regimens, and in polymorphonuclear cells, in both single- clarithromycin only and multiple dose regimens. We also observed a reduction in mononuclear cell counts in single and multiple doses. The drugs seem to reverse the mononuclear and polymorphonuclear cell ratio. An increase in oxidative burst was observed in animals treated with the drugs administered either individually or combined. In conclusion, clofazimine and clarithromycin change WBCs counts. Our results may contribute for a better understanding of the mechanisms related to the effects of co-administrating the two drugs.Clofazimina e laritromicina são utilizadas no tratamento da hanseníase e em infecções causadas pelo complexo Mycobacterium avium. Devido à escassez de dados sobre a toxicidade de esquemas terapêuticos que associam estes fármacos, este estudo teve por objetivo avaliar os efeitos adversos desta terapia, em ratos machos Wistar, por meio da determinação da contagem global e específica de leucócitos e ensaios de fagocitose e burst oxidativo de neutrófilos por citometria de fluxo. Houve aumento do número de leucócitos (dose múltipla) e de células polimorfonucleares (doses única e múltipla) nos grupos tratados com claritromicina em monoterapia ou associada à clofazimina e redução das células mononucleares, em doses única e múltipla, nos mesmos grupos. Os fármacos parecem inverter a proporção entre células mono e polimorfonucleares. Observou-se aumento do burst oxidativo nos animais tratados com os fármacos isolados ou associados. Concluindo, clofazimina e claritromicina provocam alterações leucocitárias e os resultados podem contribuir para melhor entendimento dos mecanismos relacionados aos efeitos da administração dos fármacos em associação.
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- 2012
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89. Metales pesados y su relación con marcadores de estrés oxidativo en fumadores crónicos
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Raquel Salazar-Lugo, Uslany Lozada, Maribel Rosales, Henry Astudillo, José Prin, Luisa Rojas de Astudillo, Luis Troccoli, and Mairín Lemus
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fumadores ,tioles totales ,hierro ,cinc ,albúmina ,leucocitos ,Medicine - Abstract
Se evaluaron las concentraciones de metales Fe, Zn, Cu, Cr, Cd y Ni y su relación con marcadores de estrés oxidativo en 50 fumadores crónicos de ambos géneros (30 y 84 años). Los fumadores se dividieron en dos grupos: 25 fumadores de 1-5 cigarrillos/día (cig/día) y 25 fumadores de más de 6 cig/día. Los niveles de metales en sangre total y en orina fueron determinados por espectrofotometría de emisión atómica con plasma inductivamente acoplado (ICPOES). Se realizaron determinaciones hematológicas y bioquímicas de proteínas totales, fraccionadas y de tioles totales. Los fumadores presentaron mayores concentraciones de Fe en sangre independientemente del número de cigarrillos fumados y menores concentraciones de Zn, Cr y Ni. No se observaron diferencias en las concentraciones de Cu en sangre. El Cd en fumadores estuvo entre 5,0-10,0 μg/L y en no-fumadores entre 0,0-5,0 μg/L. No se observaron diferencias signi cativas en las concentraciones de hemoglobina, hematocrito y la hemoglobina corpuscular media en los grupos, ni entre las concentraciones de albúmina ni globulinas; se observó un incremento en los leucocitos y en los grupos tioles totales de fumadores. Las concentraciones de Fe en sangre se correlacionaron con los glóbulos blancos y con el Cd; igualmente se encontró correlación entre la albúmina y los grupos tioles. Las concentraciones de Zn y el Cu se incrementaron en la orina de fumadores, no así las concentraciones de Fe. De acuerdo a estos resultados, en los fumadores crónicos, la distribución de Fe en el organismo juega un papel central en la posible progresión y desarrollo de las enfermedades relacionadas con este hábito.
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- 2015
90. Hematologic and immunological indicators are altered by chronic intake of flaxseed in Wistar rats Indicadores hematológicos e inmunológicos alterados por el consumo crónico de linaza en ratas Wistar
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L. Ferreira Medeiros de França Cardozo, L. Leal Soares, L.H. Cardozo Brant, M. Alves Chagas, V. Alves Pereira, L. G. Coca Velarde, and G. Teles Boaventura
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Peso corporal ,Linaza ,Indicadores hematológicos ,Leucocitos ,Ratas ,Body weight ,Flaxseed ,Hematologic indicators ,Leukocytes ,Rats ,Nutritional diseases. Deficiency diseases ,RC620-627 - Abstract
This work sought to evaluate the effects of chronic intake of flaxseed upon hematologic parameters and immunological findings on body development of Wistar rats. Female Wistar rats were used after gestation. They were randomly assigned into two groups during lactation period: Control group (CG), fed with casein based diet, made up of 17% protein and flaxseed group (FG), fed with casein based diet with the addition of 25% flaxseed. At weaning, 12 male pups of each group continued to receive the experimental diets of their mothers (with only 10% of protein) until adult age, when they were killed at 250 days of life aiming at blood collection. At 250 days old FG presented significant reduction in body mass (p < 0.000) and higher levels of hemoglobin (p = 0.019) and albumin (p = 0.030) than CG. It was observed smaller percentage of segmented lymphocytes (p = 0.016) in rats from FG and bigger percentage of segmented leucocytes (p = 0.023) when compared to CG. The chronic consumption of flaxseed altered hematologic and immunological indicators in adult Wistar rats. Supplementation with flaxseed seems to be beneficial to maintenance or reduction of body mass.Este trabajo pretendía evaluar el efecto de la ingestión crónica de linaza sobre parámetros hematológicos y hallazgos inmunológicos del desarrollo corporal de ratas Wistar. Se emplearon ratas hembra Wistar tras la gestación. Se las distribuyó al azar en dos grupos durante el período de lactancia: grupo control (GC), alimentado con una dieta basada en caseína, con un 17% de proteína y el grupo linaza (GF), alimentado con una dieta basada en caseína con la adición de un 25% de linaza. En el destete, 12 ratas macho continuaron recibiendo las dietas experimentales consumidas por sus madres (con sólo el 10% de proteína) hasta la edad adulta, en que fueron sacrificados a los 250 días de vida para la recogida de las muestras. A los 250 días de edad, el GF presentaba una reducción significativa de la masa corporal (p < 0,000) y mayores concentraciones de hemoglobina (p = 0,019) y albúmina (p = 0,030) que el GC. Se observó un menor porcentaje de linfocitos segmentados (p = 0,016) en las ratas del GF y un mayor porcentaje de leucocitos segmentados (p = 0,023) en comparación con el GC. El consumo crónico de linaza alteró los indicadores hematológicos e inmunológicos en las ratas Wistar adultas. La suplementación con linaza parece ser beneficiosa en el mantenimiento o la reducción de la masa corporal.
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- 2011
91. Composto homeopático reduz a liberação de ânion superóxido pelas células mononucleares de ema (Rhea americana)
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W.R Bertoldo, J.L França, L.T.O Fernandes, and A.C França-Botelho
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ema ,estresse ,superóxido ,leucócitos ,Rhea americana ,stress ,superoxide ,leukocytes ,Animal culture ,SF1-1100 - Abstract
The action of the "Stress Factor Ostrich (Arenales - Fauna and Flora)" was tested in the release of superoxide anion by cells in the peripheral blood of rhea (Rhea americana). Sixteen samples of 0.5mL of venous blood were collected through the jugular vein in the morning and placed in heparinized tubes. The leukocytes were separated into polymorphonuclear (PMN) and mononuclear (MN). The production of superoxide anion by phagocytes of peripheral blood was determined using the chromogen ferricytochrome C. There was a reduction of superoxide by MN cells in the presence of "Stress Factor Ostrich" indicating a positive influence of product against oxidative stress. Furthermore, future researches, such as the evaluation of other reactive oxygen intermediates and antioxidant enzymes, researches.
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- 2011
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92. Hematological profile of beef cattle with divergent residual feed intake, following feed deprivation
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Rodrigo da Costa Gomes, Renata Farinelli de Siqueira, Michael Alan Ballou, Tiago Roberto Stella, and Paulo Roberto Leme
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Bos indicus ,jejum ,eficiência alimentar ,leucócitos ,estresse ,Agriculture (General) ,S1-972 - Abstract
The objective of this work was to characterize the hematological profiles of steers and bulls, according to residual feed intake (RFI), after feed deprivation. Twenty‑month‑old Nellore steers and bulls were fed feedlot diets for 70 and 56 days, respectively. RFI was calculated as the difference between actual feed intake and expected feed intake. More and less efficient steers and bulls, according to RFI, were subjected to 24 hours of food deprivation. Blood was sampled prior to and following the withdrawal period. Hematological analyses included total and differential white blood cell count, red blood cell count and morphology, and plasma glucose concentration. Variation in RFI did not influence plasma glucose concentration or blood cell profiles. Glucose concentrations in bulls decreased from 114 to 97 mg dL‑1, but remained unchanged in steers, and the neutrophil:lymphocyte ratio increased from 0.39 to 0.57 following deprivation. Hematological profiles do not differ between more and less efficient steers and bulls, according to RFI.
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- 2011
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93. Presença de formas amastigotas de Leishmania chagasi e perfil leucocitário no aparelho reprodutivo de cães
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Ariane P. Benites, Carlos E. Fernandes, Karine B. Brum, and Maria Auxiliadora G.S. Abdo
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Leishmaniose visceral canina ,Leishmania ,leucócitos ,epidídimo ,testículo ,Veterinary medicine ,SF600-1100 - Abstract
A leishmaniose visceral (LV) é uma zoonose causada pelo protozoário Leishmania (Leishmania) chagasi. A leishmaniose visceral canina (LVC) é a doença de maior relevância zoonótica. Usualmente, a infecção ocorre entre um hospedeiro invertebrado para um hospedeiro vertebrado, entretanto, a transmissão na ausência do vetor já é conhecida. O objetivo principal deste estudo foi identificar a presença de formas amastigotas, quantificar as células leucocitárias, estimar o risco relativo da presença de formas amastigotas no aparelho reprodutivo de cães sorologicamente positivos com e sem sinais clínicos. Para isso, foram utilizados cães sem raça definida, sexualmente maduros e testados sorologicamente para LVC (com sinais clínicos, n=25; sem sinais clínicos, n=25), que após eutanásia, tiveram fragmentos de testículo, epidídimo (cabeça, corpo e cauda) e glândula prostática (selecionados ao acaso) impressos em lâminas. Um grupo de 20 cãs sorologicamente negativos e sem sinais clínicos foi usado como controle. Amostras do baço foram incluídas como controle parasitológico positivo. O percentual de linfócitos foi superior (P
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- 2011
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94. Os leucócitos e a resposta inflamatória na lesão de isquemia-reperfusão Leukocytes and the inflammatory response in ischemia-reperfusion injury
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Ieda Francischetti, José Bitu Moreno, Martin Scholz, and Winston Bonetti Yoshida
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Circulação extracorpórea ,Leucócitos ,Isquemia ,Traumatismo por reperfusão ,Extracorporeal circulation ,Leukocytes ,Ischemia ,Reperfusion injury ,Surgery ,RD1-811 ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Os eventos de isquemia-reperfusão desencadeiam uma resposta inflamatória sistêmica que pode levar a lesões celulares e até falência de órgãos. Tais repercussões são notadas no pós-operatório de cirurgias, em especial, com o uso de circulação extracorpórea. Sabe-se, atualmente, que os leucócitos exercem importante papel neste processo. Assim, este estudo aborda o papel dos leucócitos na fisiopatologia das lesões de isquemia-reperfusão e a ativação das cascatas inflamatórias por esse processo e procura auxiliar na compreensão destes mecanismos assim como trazer contribuições acerca das abordagens terapêuticas que possam atenuá-los. Esta revisão bibliográfica retrospectiva foi realizada a partir de documentos científicos publicados nos últimos dez anos, em português e inglês, indexados em bases de dados internacionais Medline e SciELO e de textos clássicos relacionados. Os descritores pesquisados foram: isquemia-reperfusão, leucócitos, resposta inflamatória, circulação extracorpórea, efeitos adversos e apoptose.The events of ischemia-reperfusion injury triggers a systemic inflammatory response and can lead to cellular injury and organ failure. Such effects are noted in the postoperative recovery, especially with the use of cardiopulmonary bypass. Nowadays, it is known that leukocytes play an important role in this process. Therefore, this study addresses the role of leukocytes in the physiopathology of ischemia-reperfusion injuries and activation of inflammatory cascades through this process and seek to help in the understanding of these mechanisms as well as to bring contributions on the therapeutic approaches that can mitigate them. This retrospective review was performed from indexed scientific papers published over the last ten years in Portuguese and English in international databases MEDLINE and SciELO and related classic texts. The descriptors investigated were: ischemia-reperfusion, leukocytes, inflammatory response, cardiopulmonary bypass, adverse effects and apoptosis.
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- 2010
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95. Características bioquímicas e hematológicas do pirarucu Arapaima gigas Schinz, 1822 (Arapaimidae) de cultivo semi-intensivo na Amazônia Biochemical and hematological characteristics of the pirarucu Arapaima gigas Schinz, 1822 (Arapaimidae) from semi-intensive culture in the Amazon
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Gustavo Valadares Fonseca Drumond, Ana Paula de Almeida Caixeiro, Marcos Tavares-Dias, Jaydione Luiz Marcon, and Elizabeth Gusmão Affonso
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Cultivo ,Eritrócitos ,Idade ,Leucócitos ,Sangue ,Culture ,Erythrocytes ,Age ,Leukocytes ,Blood ,Science (General) ,Q1-390 - Abstract
O presente estudo comparou os níveis plasmáticos de glicose, proteínas totais, uréia, triglicerídeos e colesterol, a contagem de eritrócitos, leucócitos e trombócitos entre alevinos e juvenis de Arapaima gigas (Osteoglociformes, Arapaimidae) de uma piscicultura semi-intensiva de Manaus, estado do Amazonas, Brasil. Os alevinos de A. gigas apresentaram níveis significativamente (p0,05) no número de trombócitos e leucócitos totais entre alevinos e juvenis. Os juvenis de A. gigas apresentaram menor quantidade de linfócitos e maior quantidade de monócitos, neutrófilos e eosinófilos, quando comparados aos alevinos. Em estudos futuros, espécimes de A. gigas com outras idades também deverão ser comparados para melhor entendimento dos efeitos da idade neste peixe amazônico, pois estas informações, além de possibilitarem uma melhor compreensão da fisiologia desta espécie, poderão auxiliar no estabelecimento de estratégias para seu manejo.The present study compared the biochemical and hematological parameters of fingerlings and juveniles Arapaima gigas (Osteoglociformes, Arapaimidae) from a semi-intensive fish farming in Manaus, Amazon State, Brazil. Fingerlings A. gigas had lower levels of total protein, triglycerides, urea and mean cell volume (MCV), but higher (p0.05) in the total thrombocytes and total white blood cell counts between fingerlings and juveniles. Juveniles A. gigas presented lower lymphocytes number and higher monocytes, neutrophils and eosinophils number when compared to the fingerlings. In further studies, A. gigas specimens with other ages should also be compared for a better understanding of the ontogenic development in this Amazon fish, because this information besides increase the knowledge of the physiology of this species, can aid in the establishment of strategies for its adequate handling.
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- 2010
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96. Evaluación del hemograma en caninos sanos sometidos a la administracion de cisplatina
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Carlos Calpa O, Carlos Daleck, and João Teotônio de Castro
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Cisplatina ,quimioterapia ,hemograma ,hemoglobina ,leucocitos ,perros ,Veterinary medicine ,SF600-1100 - Abstract
Objetivo. Evaluar los valores medios del hemograma en perros sanos durante la quimioterapia con cisplatina. Materiales y métodos. Se evaluaron los parámetros del hemograma de ocho caninos sanos sometidos a terapia con cisplatina, distribuidos en dos grupos. A los perros del grupo uno se les administró cisplatina (70 mg/m², i.v.) y para prevenir la nefrotoxicidad recibieron suero fisiológico al 0.9% (25mL/kg/h, i.v.). Los animales del grupo dos no recibieron cisplatina. Resultados. El hemograma de los perros del grupo uno no mostró diferencia significativa (p>0.05) con respecto al de los animales del grupo dos. Conclusiones. Los datos obtenidos indicaron que el protocolo con cisplastina no alteró significativamente los valores medios del hemograma y el número de sesiones de quimioterapia aumenta el riesgo de disminución de los valores del hemograma.
- Published
- 2010
97. Parâmetros hematológicos de espécies nativas do rio Tocantins, Auchenipterus nuchalis, Psectrogaster amazonica e Squaliforma emarginata (Teleostei, Ostariophysi) = Hematological parameters of native fish species of the Tocantins river, Auchenipterus nuchalis, Psectrogaster amazonica and Squaliforma emarginata (Teleostei, Ostariophysi)
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Elianora Gomes de Carvalho, Carla Simone Seibert, Michella Soares Coelho, and Elineide Eugênio Marques
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peixes ,hematologia ,eritrócitos ,morfometria ,leucócitos ,rio Tocantins ,fish ,hematology ,erythrocyte ,morphometry ,leucocytes ,Tocantins river ,Biology (General) ,QH301-705.5 ,Microbiology ,QR1-502 - Abstract
Neste estudo, foram avaliadas as características hematológicas e a morfometria de Auchenipterus nuchalis, Psectrogaster amazonica e Squaliforma emarginata, capturados no rio Tocantins. As amostragens foram realizadas no rio Tocantins, onde o sangue foi coletadopor punção caudal dos animais, determinando-se hemograma completo e morfometria dos eritrócitos. Auchenipterus nuchalis (n = 15) apresentou maior número de eritrócitos, hemoglobina e hematócrito, seguido de P. amazonica (n = 26) e S. emarginata (n = 26). Otamanho dos eritrócitos foi maior para S. emarginata, mostrando que quanto maior o tamanho dos eritrócitos, menor o número de células circulantes. Squaliforma emarginata também apresentou maior número de blastos (eritrócitos ortocromáticos e policromatófilos) no sangue periférico. Estas diferenças podem ser atribuídas a adaptaçõesrespiratórias de natureza comportamental e/ou fisiológicas, relacionadas ao meio ambiente. O maior percentual de linfócitos foi observado em P. amazonica; o de monócitos e neutrófilos, na espécie A. nuchalis. A variação nos percentuais de leucócitos entre as espécies pode estar relacionada ao nicho ocupado, favorecido pela exposição a diferentes microrganismos.This study evaluated the hematological characteristics anderythrocyte morphometry in Auchenipterus nuchalis, Psectrogaster amazonica and Squaliforma emarginata captured in Tocantins river. The samples were taken in the Tocantins river, where blood was collected by puncturing the caudal vein of the animals. The completehemogram and morphometry of the erythrocytes were determined. Auchenipterus nuchalis (n = 15) showed the largest number of erythrocytes, hemoglobin and hematocrit, followed by P. amazonica (n = 26) and S. emarginata (n = 26). Squaliforma emarginata showed the largest erythrocytes and the smallest number of circulating cells. Squaliforma emarginata also showed the highest number of circulating blast cells (orthochromatic and polychromatophil erythrocytes) in the peripheral blood. These differences can be attributed to respiratoryadaptations of a behavioral or physiological nature related to the environment. The highest percentage of lymphocytes was observed in P. amazonica, and of monocytes and neutrophils in A. nuchalis. The variation among the species in the percentages of leucocytes can also be attributed to the niche occupied, favored by exposure to different microorganisms.
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- 2009
98. Plaqueta e leptina em adolescentes com obesidade Platelet and leptin in obese adolescents
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Denis Foschini, Ronaldo V. T. dos Santos, Wagner L. Prado, Aline de Piano, Mara C. Lofrano, Aniela C. Martins, June Carnier, Danielle A. Caranti, Priscila de L. Sanches, Lian Tock, Marco T. de Mello, Sérgio Tufik, and Ana R. Dâmaso
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Adolescentes ,obeso ,não-obeso ,leucócitos ,cortisol ,leptina ,Adolescents ,obese ,non-obese ,leukocytes ,leptin ,Pediatrics ,RJ1-570 - Abstract
OBJETIVOS: Analisar a influência de obesidade na contagem de células imunológicas e na concentração dos hormônios cortisol e leptina, a fim de estabelecer uma relação entre as variáveis analisadas. MÉTODOS: Foram recrutados 27 adolescentes obesos [índice de massa corporal (IMC) ≥ percentil 95[ e 21 não-obesos (IMC ≤ percentil 75), de ambos os sexos, com idade entre 15 e 19 anos, na fase pós-púbere. O IMC foi calculado através da divisão do peso pela altura ao quadrado e a composição corporal foi estimada por pletismografia no sistema Bod PodTM. Amostras de sangue foram colhidas para análise de leucócitos, neutrófilos, linfócitos, monócitos, plaquetas, cortisol e leptina. O teste de Kolmogorov-Smirnov foi utilizado, seguido pelo teste t de Student independente supondo distribuição normal. O nível de significância estabelecido foi p < 0,05 e expresso como média ± desvio padrão. Os dados foram analisados utilizando o programa SPSS para Windows versão 12.0. RESULTADOS: Não houve diferença entre o grupo obeso e o não-obeso na concentração sérica de leucócitos, neutrófilos, linfócitos, monócitos e cortisol. O grupo de adolescentes obesos apresentou maiores concentrações de plaquetas e leptina (p < 0,01). A prevalência de hiperleptinemia foi de 25,92% nos adolescentes obesos (15,38%, sexo masculino e 35,7%, feminino). CONCLUSÕES: Os adolescentes obesos apresentaram maiores concentrações de plaquetas e leptina em comparação aos não-obesos. Observou-se que as adolescentes obesas apresentaram maior prevalência de hiperleptinemia do que os adolescentes obesos.OBJECTIVE: To analyze the influence of obesity status on immune cell count and concentration of the hormones cortisol and leptin, in order to establish a relationship among the variables analyzed. METHODS: We recruited 27 obese [body mass index (BMI) ≥ 95th percentile[ and 21 non-obese (BMI ≤ 75th percentile) adolescent boys and girls, aged 15-19 years at the post-pubertal stage. BMI was calculated as body weight divided by height squared, and body composition was estimated by plethysmography in the Bod PodTM system. Blood samples were collected to analyze leukocytes, neutrophils, lymphocytes, monocytes, platelets, cortisol, and leptin. The Kolmogorov-Smirnov test was performed, followed by the independent Student t test in case of normal distribution. Significance values were set at p < 0.05 and expressed as means ± standard deviation. The statistical package SPSS for Windows version 12.0 was used. RESULTS: There was no difference between obese and non-obese adolescents in terms of leukocyte, neutrophil, lymphocyte, monocyte and cortisol serum concentrations. The group of obese adolescents presented higher platelet and leptin concentrations (p < 0.01). The prevalence of hyperleptinemia was 25.92% in the obese adolescents (15.38% in boys and 35.7% in girls). CONCLUSIONS: Obese adolescents have higher platelet and leptin concentrations in comparison with non-obese adolescents. It was also found that obese girls presented a higher prevalence of hyperleptinemia than obese boys.
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- 2008
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99. Comparative study on hematological parameters of farmed matrinxã, Brycon amazonicus Spix and Agassiz, 1829 (Characidae: Bryconinae) with others Bryconinae species Estudo comparativo sobre parâmetros hematológicos de matrinxã Brycon amazonicus Spix e Agassiz, 1892(Characidae: Bryconinae) criados em cativeiro, com outras espécies de Bryconinae
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Marcos Tavares-Dias, Elizabeth Gusmão Affonso, Sarah Ragonha Oliveira, Jaydione Luiz Marcon, and Mizue Imoto Egami
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Leucócitos ,peixe de água doce ,sangue ,Amazônia ,Brycon amazonicus ,Bryconinae ,Leukocytes ,Freshwater fish ,Blood ,Amazon ,Science (General) ,Q1-390 - Abstract
The aim of this study was not only to determine the red blood cells parameters, thrombocyte and leukocyte counts in farmed Brycon amazonicus (matrinxã), to compare these parameters among Bryconinae species from literature, and also to investigate the presence of special granulocytic cells in these fish. The results of the blood cells parameters here established for farmed B. amazonicus, a species of great economic importance in Brazilian aquaculture, could help a better understanding of the blood features in natural populations of this Amazon species. Blood parameters varied between Bryconinae species investigated, mainly the red blood cell counts, hemoglobin, hematocrit and mean corpuscular volume (MCV). The presence of the blood granulocytes, neutrophils and heterophils in matrinxã suggest that both leukocytes can be a characteristic for Bryconinae family. Furthermore, it indicates that the existence of special granulocytic cells in the blood of Bryconinae species from literature is an artifact, and this was herein discussed.O objetivo deste estudo foi determinar os parâmetros eritrocíticos, as contagens de trombócitos e de leucócitos de espécimes de Brycon amazonicus (matrinxã), criados em cativeiro e compará-los com aqueles descritos na literatura para outras espécies de Bryconinae. Além disso, foi ainda investigada a presença de células granulocíticas especiais nestes peixes. Os resultados dos parâmetros sangüíneos apresentados para B. amazonicus podem ajudar a entender melhor as características sangüíneas em população natural desta espécie de grande importância para a aqüicultura brasileira. Os parâmetros sangüíneos das espécies de Bryconinae investigadas apresentaram variação interespecíficas principalmente a contagem de eritrócitos, hemoglobina, hematócrito e volume corpuscular médio (VCM). A presença dos granulócitos sangüíneos, neutrófilos e heterófilos em matrinxã sugere que esta pode ser uma característica das espécies pertencentes à subfamília Bryconinae. Além disso, indica que a existência de células granulocíticas especiais no sangue de espécies de Bryconinae da literatura é um artefato, e isto foi aqui discutido.
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- 2008
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100. Característica leucocitária, relação albumina/globulina, proteína plasmática e fibrinogênio de bovinos da raça Nelore, confinados e terminados a pasto Leukocyte characteristic, albumin/globulin relation, plasmatic protein and fibrinogen of bovines of the Nelore race confined and grazing
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Ediane Batista da Silva, Maria Clorinda Soares Fioravanti, Luiz Antônio Franco da Silva, Eugênio Gonçalves de Araújo, Liliana Borges de Menezes, Marina Pacheco Miguel, and Dirson Vieira
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criação extensiva ,bovinos ,leucócitos ,proteínas plasmáticas ,bovine ,feedlot ,leukocytes ,plasma proteins ,Agriculture ,Agriculture (General) ,S1-972 - Abstract
Esse trabalho avaliou as mudanças na contagem de leucócitos e algumas proteínas séricas de bovinos confinados e terminados a pasto. De 120 amostras sangüíneas coletadas, 60 foram obtidas de bovinos Nelores machos confinados e 60 de animais com as mesmas características, porém manejados extensivamente. As amostras foram obtidas por ocasião do abate desses animais. Os parâmetros estudados foram contagem de leucócitos, razão albumina/globulina e concentração de fibrinogênio plasmático. Na análise dos dados empregou-se estatística descritiva, obtendo-se as médias, desvio padrão e coeficiente de variação para todos as variáveis avaliadas e posteriormente comparou-se as médias por meio de teste não-paramétrico. Os bovinos terminados a pasto apresentaram maior nível de globulina e fibrinogênio (P>0,05) quando comparados com os confinados (globulina: pastejo=3,29g dL-1 0,76; confinamento 2,99g dL-1±0,60 e Fibrinogênio: pastejo=872mg dL-1±610; confinamento=633mg dL-1±319). O número de leucócitos total foi de 7,64±2,15 em bovinos confinados e de 7,72±1,84 nos terminados a pasto. Não houve diferença (P>0,05) entre essa variável e a contagem diferencial de leucócitos bem como na proteína sérica total (g dL-1) dos bovinos terminados a pasto (6,10±0,53) e dos confinados (5,96±0,49). O nível de albumina dos bovinos confinados (3,01g dL-1±0,43) e a razão A/G (1,07±8,91) foram maiores quando comparados com os bovinos terminados a pasto (2,82g dL-1±0,45) e (0,95±0,38) respectivamente. O nível mais elevado de albumina nos bovinos confinados sugere que eles foram submetidos a uma dieta nutricional mais adequada. O constante desafio imunológico sofrido pelos animais terminados a pasto pode ter sido responsável pelo elevado nível de globulina e fibrinogênio. Esses resultados indicaram que, apesar das adversidades que os bovinos confinados são submetidos, eles não apresentaram alterações correlacionadas com esse fato.This research aimed to evaluate the changes in the white blood cell count and some serum proteins of confined cattle (CC) and grass cattle (GC). From the 120 blood samples collected, 60 were obtained from confined Nelore male bovines and 60 from animals with the same characteristics but managed extensively. Samples were obtained at the moment of slaughter. Parameters studied were the white blood cell count, serum albumin/globulin ratio and concentration of plasma fibrinogen. Descriptive statistics was used in the analysis of the data, and the averages, standard deviation and coefficient of variation calculated for all parameters evaluated. The comparisons between averages were made by non-parametric test. The grazing cattle showed higher levels of globulin and fibrinogen when compared to the confined ones (globulin: GC=3.29g dL-1±0.76; CC=2.99g dL-1±0.60 and Fibrinogen: GC=872mg dL-1±610; CC=633mg dL-1±319). The total number of white blood cells mL-1 was 7.64±2.15 in confined cattle and 7.72±1.84 in grazing cattle. There was no significant difference between this variable and differential white blood cell count as well as the total serum protein (g dL-1) from grazing cattle (6.10±0.53) and confined cattle (5.96±0.49). The level of albumin from confined cattle (3.01g dL-1± 0.43) and the A/G ratio (1.07±8.91) were greater when compared to the grazing bovines (2.82g dL-1±0.45) and (0.95±0.38) respectively. The higher serum levels of albumin found in confined herd suggest that they were subjected to a more adequate nutritional diet. The constant immunological challenge suffered by the GC could be responsible for the elevated serum levels of globulin and fibrinogen. These results showed that although feedlots present a stressful environment they did not show any blood alterations correlated to this fact.
- Published
- 2008
- Full Text
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