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This study aims to examine the effect of gamification on perceived playfulness, learning engagement, and learning motivation. In addition, another aim of this research is to examine the effect of perceived playfulness, learning engagement, and learning motivation on academic achievement. This research was conducted in the context of hybrid learning. The method used in this study is a quantitative method, using primary data as a data source. Questionnaires are used in data collection methods, which are distributed online using Google Form. Respondents in this study amounted to 100. The sampling technique used simple random sampling—a data analysis tool using SmartPLS. The results of the study show that gamification has an effect on perceived playfulness, learning engagement, and learning motivation. In addition, this study also proves that learning engagement and learning motivation have a significant and positive effect on academic achievement. Another finding from this research is that perceived playfulness has no effect on academic achievement.

The widespread use of pesticides for agricultural purposes results in their presence in the environment. Pesticide occurrence is causing potential negative impacts on human health and environmental deterioration in general. One of the interdisciplinary field to implement a technology for converting and conserving resources is bioelectrochemistry. An integrated system based on bioelectrochemical processes, namely Bioelectrochemical System (BES), has been well developed to date. The application of BES to remove various materials in the bioremediation process has been well established in the combination with diverse technologies. The set up of integrated BES with Granular Activated Carbon (GAC) is one of the promising solutions to enhance the removal of the pesticide in water. The BES in situ has been implemented to remove contaminants in groundwater. This mini-review discusses the recent development of pesticide removal and environmental remediation using bioelectrochemical approaches, offering new insight into the big scope of green technology application.

Human erythropoietin (hEPO) is a glycoprotein that regulates the formation of erythrocytes and mainly used in anemia patients. Previously, we have reported the expression of modified human EPO with 2 additional N-linked in mammalian cell CHO-K1. The aim of this current research was to study the optimum condition for modified recombinant hEPO (rhEPO) production in CHO-K1. To do this, several parameters of culture conditions were applied including antibiotic concentrations, seeding densities, time of incubations, fetal bovine serum (FBS) concentrations and cell culture media. The result showed that the presence of antibiotic G418 improved the expression level with the highest was at 1% of concentration. Meanwhile, seeding density of 2–3x105 cells/6 cm dish and seven day of incubation time were the best condition for rhEPO protein expression. From five different combination media used, F12 medium with 10% FBS gave the highest expression of rhEPO protein. From this study was also found that at passage 16 the expression level was still increasing proving that the clone expressing the protein of our interest is promisingly stable. Keywords : EPO, erythropoietin, protein expression, CHO-K1, optimation

Abstrak TNGM memiliki jenis ekologi yang unik, ekologi di wilayah sekitar gunung merapi seringkali mengalami perubahan ekosistem. Sejalan dengan kedinamisan ekosistem TNGM yang dipaparkan sebelumnya maka vegetasi yang berubah perlu diidentifikasi dan dianalisis untuk mengetahui keanekaragaman dan kondisi ekosistemnya pasca erupsi 2010. Metode pengambilan data pada penelitian ini dilakukan dengan observasi langsung menggunakan pembuatan petak kuadrat, analisis vegetasi, quadrat sampling techniques dan prinsip representative. Analisis data yang digunakan adalah analisis deskriptif hasil observasi. Hasil penelitian ini adalah wilayah observasi memiliki 4 habitus utama yaitu lumut, herba, semak, dan tegakan. Dominansi dengan indeks nilai penting (INP) yang tertinggi pada setiap habitus meliputi Bryopsida sp. dengan INP 300% untuk lumut , Viburnum sp. dengan INP 87.43% untuk herba, Carex sp. dengan INP 132.63% untuk semak, dan Acacia sp. dengan INP 300% untuk tegakan. Sedangkan speies dengan dominasi paling rendah pada setiap habitus meliputi Euphorbia sp. dengan INP 8.97% untuk herba dan Ageratina sp. Dengan INP 37.31% untuk semak. Kata kunci: TNGM, Vegetasi, quadrat sampling techniques Abstract TNGM has unique type of ecology, the ecology in the area around Mount Merapi often undergoes ecosystem changes. In line with the dynamics of the TNGM ecosystem described before, changing vegetation needs to be identified and analyzed to find the diversity and condition of the ecosystem after the 2010 eruption. Data collection methods in this study were carried out by direct observation using quadratic plot making, vegetation analysis, quadrat sampling techniques and representative principles. Analysis of the data is descriptive analysis of observations. The results of this study are the observation area has 4 main habitus namely mosses, herbs, shrubs, and stands. Dominance with the highest importance value index (INP) in each habitus includes Bryopsida sp. with 300% INP for moss, Viburnum sp. with INP 87.43% for herbs, Carex sp. with INP 132.63% for shrubs, and Acacia sp. with a 300% INP for stands. While the species with the lowest dominance in each habitus include Euphorbia sp. with INP 8.97% for herbs and Ageratina sp. With INP 37.31% for shrubs. Keywords: TNGM, Vegetation, quadrat sampling techniques

This study aimed to investigate the efficacy of a newly developed NK3 receptor agonist (B21-750) on the secretion of luteinizing hormone (LH) in association with ovarian steroid hormones during the follicular phase (FP, n = 5) and luteal phase (LP, n = 5) of Shiba goats. The FP group was treated with both prostaglandin F2α and progesterone-controlled internal drug release (CIDR) inserts for 10 d, and B21-750 (200 nmol) was injected 12 h after removing the CIDR. Meanwhile, the LP group received B21-750 injections on a day during the mid-luteal phase. LH secretion increased at 1 h after B21-750 injection in both groups. The percent changes in the area under the curve of LH was higher during the hour after injection than during the hour before injection in both groups. Thus, this study demonstrated that B21-750 induces rapid LH secretion for a short period during both the follicular and luteal phases.

Data BPS untuk tiga tahun terakhir menunjukkan bahwa petani Indonesia sudah sejahtera dengan nilai tukar petani mencapai >100. Tujuan penelitian ini adalah menganalisis pengaruh peran gender dan interaksi suami-istri terhadap indeks kebahagiaan keluarga petani. Pendekatan penelitian adalah kuantitatif dengan desain cross-sectional study. Pengumpulan data primer menggunakan teknik wawancara melalui kuesioner. Pada penelitian ini, sampel dipilih secara purposive di Kecamatan Pamijahan, Kabupaten Bogor, Provinsi Jawa Barat. Kriteria suami adalah bekerja sebagai petani padi sawah serta istri bekerja yang menghasilkan uang. Jumlah sampel 70 orang. Hasilnya, kategori untuk variabel peran gender, interaksi suami-istri, serta indeks kebahagiaan keluarga petani adalah sedang. Ada perbedaan peran gender serta interaksi suami-istri keluarga petani setiap masanya. Data uji regresi menunjukkan bahwa ada pengaruh usia suami, pendapatan per kapita keluarga, serta interaksi suami-istri terhadap indeks kebahagiaan keluarga petani. Semakin tinggi usia suami, semakin besar pendapatan per kapita dan semakin intens interaksi suami-istri, semakin sejahtera keluarga petani. Implikasi penelitian adalah perlunya peningkatan indeks kebahagiaan petani melalui peningkatan pendapatan per kapita dan interaksi keluarga.

Tax is the biggest source of APBN income in Indonesia. Various payment applications use the e-billing method or manually. A guide for learn about the effect of applying the e-billing payment method and tax payment slip (SSP) to the understanding of taxpayers. This research is quantitative research. Data collection techniques by giving questionnaires to consumers, conduct observations, interviews, and study relevant literature. The sampling technique uses probability sampling so that a sample of 70 from PT. Kartini Patent. The results showed that there was no significant effect between the e-billing payment methods on the understanding of taxpayers, while the positive and significant effect was shown on the method of manual payment of tax payments (SSP) on mandatory understanding. Based on the results of correlation analysis, this study obtained a value of 0.119 or 11.9% which means it has a very low relationship between the independent and dependent variables.

This study aims to find out how the role of the Family Planning Population Control Office, the determination of policies and programs in controlling the population growth rate in Medan City, besides that the author also wants to know the factors that prevent the Population and Family Planning Control Office from controlling growth residents in Medan City. The research technique used is a qualitative research method with interviews and observations. The research subject taking technique is using purposive and snowball techniques. From this technique, informants obtained, consisting of the Head of the Population and Family Planning Control Office, Head of the Family Planning Division, Head of Extension and Penggerak, Head of Resilience, Head of Population Control, Family Planning Field Extension, and the community who participated in the Family program. Planning or those who do not take part in the Family Planning program. Based on the research conducted, it was found several findings that the Office of Population and Family Planning Control has a role in suppressing population growth which includes coordination with government, community and private institutions in family planning activities, conducting family planning counseling, installing contraceptives and post-installation contraception. Obstacles in controlling population growth, namely the lack of skilled technical personnel, inadequate infrastructure, the influence of culture and the level of education of the community also become obstacles to DP2KB in carrying out their duties and functions.

Kasus penganiayaan tak henti-hentinya menjadi sorotan publik, tak hanya penganiayaan yang dilakukan secara spontan, namun juga yang direncanakan terlebih dahulu dan menimbulkan luka berat sebagaimana yang diatur di dalam Pasal 353 ayat 2 KUHP. Seperti yang kita ketahui, penganiayaan berencana sendiri merupakan salah satu tindak pidana. Di mana, terdapat dua unsur di dalamnya antara lain: Actus Reus yaitu suatu perbuatan yang menurut masyarakat tercela dan patut dihukum, dan Mens Rea atau niat jahat pelaku. Adapun keterkaitan keduanya dapat dilihat dalam bentuk ekspresi “actus non reus facit reum nisi mens sit rea”. Secara konseptual, tergambar secara jelas penentuan niat jahat (mens rea) di dalam suatu perbuatan (actus reus) khususnya tindak pidana penganiayaan berencana yang mengakibatkan luka berat, namun nyatanya dalam tatanan implementasi, hal ini masih menjadi menimbulkan perdebatan, yaitu terdapat perbedaan penafsiran serta penerapan unsur mens rea dalam tindak pidana penganiayaan berencana. Penelitian ini merupakan penelitian hukum normatif melalui pendekatan perundang-undangan dengan menggunakan sumber data sekunder berupa bahan hukum primer, sekunder, dan tersier. Sedangkan spesifikasi penelitian ini adalah deskriptif analitis. Bahan hukum diperoleh melalui studi dokumen atau kepustakaan, yang kemudian dianalisis secara kualitatif, komprehensif, dan lengkap. Di dalam penelitian ini akan dikaji mengenai penafsiran dan penerapan unsur mens rea pada tindak pidana penganiayaan berencana dari berbagai putusan, sehingga kesimpulan dari penelitian ini untuk memberikan penjelasan mengenai penentuan mens rea yang diterapkan pada masing-masing kasus, serta memberikan pemahaman dan saran guna menentukan parameter untuk mengukur mens rea pada perkara penganiayaan berencana yang mengakibatkan luka berat sebagaimana Pasal 353 ayat 2 KUHP. Abstract Persecution cases, not just spontaneously but also planned and resulting in severe injury, regulated in Criminal Code (KUHP) Article 353 Paragraph 2, are continuously gaining public’s attention. As we have known, Actus Reus is an act that in society’s perspectives are disgraceful be punishable. Actus Reus heavily related to Mens Rea or malicious intent of the perpetrators. Both connections can be seen in the expression of “actus non reus facit reum nisi mens sit rea”. Conceptually, the malicious intent of perpetrators (mens rea) are clearly shown in an act (actus reus), especially a planned persecution crime that causing a heavy injury. But in real implementation, this case is still on debate. There are different interpretation and application of mens rea factor in planned persecution crimes. This research is normative study through law approach with secondary data in a form of primary, secondary and tertiary law and legal sources. However, the specification of this research is a descriptive analysis. Law and legal sources obtained from literature studies that are analyzed completely and comprehensively. This research would examine the interpretation and application of mens rea factors in planned persecution crime from different verdicts. So the conclusion of this research will give clearer explanation to determine mens rea in each cases and give deeper understanding and suggestion to define a parameter to mens rea in severe injury-planned persecution crime based on Article 353 Paragraph 2.

This research aimed to describe the solution model for child marriage. This research took place in Malang Regency. The subjects in this research were married couples who married at the age of children, parents from the woman’s side (wife), and community leaders. Research data obtained through observation and in-depth interviews, then analyzed with interactive techniques. The impact of child marriage that occured in Malang Regency caused the family to not function properly, so a solution was needed to overcome it. “Gerakan Sadar Kespro” was a solution model for the phenomenon of child marriage by integrating education, health, counseling, and life skill development services. “Gerakan Sadar Kespro” was implemented in two stages of intervention, including prevention intervention and intervention on the impact of child marriage. Integration of education and health services was a preventive strategy to prevent the practice of child marriage. At the stage of the impact on child marriage, the integration between counseling services and the development of life skills was in the domain of curative strategies for perpetrators of child marriage and their parents.

This study aims to describe and analyze the motivations of using internet banking with gender as the moderation to representation the consumer behavior which can provide design of marketing strategies based on customers’ desires and needs. The sampling techniques are a purposive and a snowball sampling with 250 respondents in Surabaya. The result shows that perceived of usefulness, perceived ease of use, perceived of security and bank initiatives are motivations that encourage using the internet banking service. However, gender as the moderation does not influence to all these factors in using internet banking services.

The aims of this research were to identify key internal and external success factors within the forwarding industry and the organization, and to analyze competitive positioning of a forwarding business in Indonesia in order to obtain its optimal business strategy. Research method applied was qualitative approach using case study. Respondents consist of 20 managers at Jakarta. Data collection was conducted in October 2017 at Jakarta using questionnaire distribution. The first stage of data analysis mapped company's strengths and weaknesses into Internal Factor Evaluation (IFE) matrix, mapped market opportunities and threats into External Factor Evaluation (EFE) matrix, and mapped competition into Competitive Profile Matrix (CPM) with the application of Expert Choice Software. The results of the external and internal audit were used to generate alternative strategies by applying a Business Competitive Position Matrix, Grand Matrix Strategy, and BCG Matrix. Three alternative strategies were selected from matrices conducted prior to Quantitative Strategic Planning Matrix (QSPM), namely adding the new sub-business unit to serve delivery service for small-sized goods (product development), conduct IT innovation to open opportunities in entering E-Commerce business (market development), and liquidation. The outcome is a product development strategy, which is indicating that the best strategic decision for the future sustainability in the business competition is to add new sub-business unit in small-sized goods delivery.

The purpose of this study is to analyze the antecedent consumer involvement and brand self expressiveness and brand community identification of the three dimensions of consumer brand engagement (CBE) (cognitive, emotional, behavior) in the luxury brand on social media. The data was collected through an online questionnaire which was filled in by 125 respondents who have experience in buying luxury brands using purposive sampling. The empirical study was conducted using structural equation modeling SmartPls. The results of the study stated that Consumer Involvement has a negative impact, brand self expressiveness and consumer brand identification have a positive impact on dimensions of CBE. Tests were also conducted to see the consequences of CBE, namely an increase in brand image and brand loyalty. Managers of luxury brand companies must increase CBE by utilizing and managing consumers following social media and creating communities to make it easier for consumers to get to know the advantages of luxury brand products. This study is a pioneer, as it extends the consumer engagement model to the social media context luxury brand by adding brand community identification as an antecedent CBE. Keywords: consumer brand engagement, brand image, brand loyalty, luxury brand, social media

In this study, gender equality does not imply complete equivalence between men and women, but rather ensures opportunities are independent of gender. Both genders have equal opportunities to fulfill their rights and potential, particularly in education. Unfortunately, not all Betawi women have the opportunity and access to advance their education. A prevailing societal stigma suggests that higher education is unnecessary for them, as their primary role is deemed to be household care. This study aims to examine the relationship between communication patterns within Betawi ethnic families and women's attitudes toward pursuing higher education. This study employs a quantitative methodology, collecting data through surveys. The study participants consisted of 254 women from Betawi ethnic families, both current higher education students and graduates. Spearman's rank analysis was conducted for data analysis, yielding Spearman's rank with a p-value of 0.737. There was a relationship between the communication patterns of Betawi ethnic families and women's attitudes toward continuing higher education, considering the perspective of gender equality in education. This study suggests that education plays a pivotal role in achieving gender justice, facilitating the transmission of norms, knowledge, and skills.

Keterampilan menulis para guru dikabarkan masih rendah. Di era teknologi sekarang ini, blog merupakan sarana strategis untuk memotivasi guru untuk menulis. Pengabdian ini berfokus pada pelatihan menulis blog bagi guru di kota Semarang. Metode pengabdian yang digunakan adalah pelatihan terfokus beserta evaluasi. Dari progam ini dihasilkan bahwa guru antusias dan dapat membuat blog secara mandiri. Guru pun telah mampu membuat tulisan pada blog pribadinya. Diharapkan program ini berkelanjutan dan pihak sekolah hendaknya memfasilitasi wifi untuk memudahkan para guru menulis di blog. Katakunci: menulis, guru, blog.

Sorosutan Subdistrict is located in Umbulharjo Subdistrict, Yogyakarta City. The development of Sorosutan Subdistrict is so rapid as indicated by the density of settlements and the complexity of the problems in it. One of these problems is related to settlement conditions. The condition of Sorosutan Subdistrict which has a slum area needs to be investigated on the quality of its settlements through assessment, weighting, and interpretation of Google Earth Imagery. The stage of determining the quality of settlements in this research is interpreting the imagery and determine the scoring of each parameter of settlement quality using GIS software. Based on the analysis of the results of data processing, it was found that the quality of settlements in Sorosutan Subdistrict is partly of medium quality of 54.27%, while good quality is 23.05% and bad quality is 22.68%. The distribution of good and bad quality settlements dominates the western and southern parts of Sorosutan Subdistrict. The distribution of settlement quality dominates the eastern part of Sorosutan Subdistrict. Hamlets which are decided as slum settlements in these qualities of settlements assessment have bad qualities in hamlet numbers 004 and 015.

The legality of business permits is important in developing Micro, Small and Medium Enterprises (MSMEs). NIB or Business Identification Number is an identity number used by business actors to obtain business permits and commercial or operational permits. Online Single Submission (OSS) is an Electronically Integrated Business Permit application issued by the OSS institution on behalf of the Minister, Agency Head, Governor, or Regent/Mayor to business actors via electronic means integrated by the system. IMAPRES, or the Association of Achievement Students in Wonogiri Regency, is a student organization that receives scholarships in Wonogiri Regency. The goal to be achieved in the Business Identification Number or NIB acceleration program is that the Wonogiri Regency is required to complete the NIB or Business Identification Number from 294 villages. The method used uses stages such as observation or survey, counseling regarding Business Identification Numbers or NIBs, and implementation of assistance and creation of NIBs or Business Identification Numbers. The Department of Investment and One Stop Integrated Services (DPMPTSP) is collaborating with IMAPRES or the Association of Achievement Students in Wonogiri Regency to complete the NIB (Business Identification Number).

The tourism sector, particularly the development of tourist villages, can be an effective tool to promote gender equality. However, women often face disparities, especially in economic opportunities and decision-making. This issue highlights the importance of this research, which aims to analyze the implementation of gender mainstreaming in tourism development in Nongkosawit Tourism Village, Semarang City. The study uses a qualitative descriptive method with the gender analysis pathway technique, focusing on four aspects: access, participation, control, and benefits. The snowball technique was used to determine the informants, with the head of the local tourism awareness group (Pokdarwis) as the key informant. The results show that Nongkosawit has begun to implement gender mainstreaming principles, particularly in terms of access and benefits. However, participation and control remain suboptimal due to the limited understanding of gender-responsive concepts in the community. As a result, women's participation in decision-making and leadership is still low, and they face difficulties accessing capital to start tourism-related businesses. The study recommends increasing gender-related education and fostering partnerships between the government, local organizations, and communities to ensure inclusive and sustainable tourism development that benefits both women and the local community.

This article aims to determine the role of family communication in motivating higher education. Public education in Papua is not yet evenly distributed as a whole due to constraints on facilities, means, or understanding the importance of education itself. This research was conducted by conducting in-depth interviews with overseas students from Papua who were continuing their higher education (or studying) in Jakarta. Based on the Symbolic Interaction theory, an action will do something because there is an understanding of the meaning. This research shows that the five students interviewed have the same sense of the meaning of education, that education is important for a better future. So they consider college (thing) as something that must be done to achieve a good future. Therefore, they chose to study in Jakarta as a form of action (act). In the end, the meaning of the importance of education and what motivates them to study in Jakarta is the family, especially parents. Parents, as the smallest institution, who have been together since childhood, have been involved in providing education and giving them views on good education and the goals of education.

Soft tofu dessert is a traditional food made from soft tofu and ginger drink. The traditional ginger drinkcontains antioxidant compounds. There are many varieties of ginger in Indonesia which have different antioxidant activities. Partially subtitution of Zingiber officinale var. Rubrum by Zingiber officinale var. Amarum was expected to improve sensory characteristics and antioxidant activity of traditional ginger drink. The objective of this research was to improve the formula of ginger drink based on sensory characteristics and antioxidant activity in the development of soft tofu dessert as a functional food. Optimization was done by Response Surface Methodology (RSM) based on the value of lightness (L) and °Hue of ginger drink which measured by Chromameter and antioxidant activity determined by DPPH method. Independent variables using in this optimization were the concentration of ginger and brown sugar. The optimal formula of ginger drink and its application in soft tofu dessert were analyzed by the hedonic rating method. The result of optimal formula consisted of 10.00% Zingiber officinale var. Rubrum, 9.99% Zingiber officinale var. Amarum, and 10.00% brown sugar with a desirability value of 0.822. The antioxidant activity of the optimal formula of ginger drink was AEAC 553.40 ppm, lightness (L) value was 40.03, and oHue was 74.67. The result of sensory test showed that the panelist preference for optimal formula was higher than for the traditional formula of ginger drink, while the acceptance level of the optimal formula was also higher than the tradisional formula of soft tofu dessert.

Purpose: Existing literature shows conflicting views regarding street vendors in a place. They are considered both positive and negative. Their existence has rarely been examined from a combination of place-making and power theories. This research aimed (a) to identify the actors who transform Borobudur Food and Craft Market and the sources of power and actions carried out by these actors and (b) to find out the views of other actors about street vendors. Design/methodology/approach: The design used in this study was an instrumental case study. The data were collected using semi-structured interviews and photo documentation. The sampling technique used was purposive sampling with several participants of as many as 12 persons consisting of 4 street vendors, 4 employees of the state-owned enterprise in charge of the Borobudur tourist site and 4 tourists. The data were analyzed through two-phased coding. To improve the credibility, participant validation was conducted. Findings: This study made new findings. First, in a place, there are formal and informal place-making actors who transform the place with the sources of power they have and the acts of power they carry out. Both formal and informal actors can perform coercive and noncoercive acts of power. This shows the existence of contested power in a setting. Second, street vendors are viewed positively as well as negatively. Comprehensive policies need to be implemented by key actors to minimize the negative sides of the existence of street vendors and optimize the benefits from them. Research limitations/implications: Data collection was carried out when the Indonesian government implemented restrictions on public activities during the pandemic. At that time, tourism activities were very limited. In the future, researchers can use other techniques such as the self-report visual method because not all street vendors are fluent in expressing their opinions in interviews. Practical implications: Both central and regional governments and local stakeholders can synergistically carry out a dialogue, seeking common ground to accommodate each other's interests. The next steps are to consistently apply the policies resulting from the dialogue and ensure that each actor plays a role according to their respective portion and authority. Originality/value: Previous studies typically explain power as the possession of resources and relational attributes. This study has taken a different point of view, namely about acts of power contested in a certain place. Actors who have power are not always those who have formal legitimacy. Informal parties are considered powerless despite having power.

The existence of university can be a new growth center area that causes affect to increased carrying capacity areas such as residential area and trigger development of commercial and service areas to support educational activities. Development uncontrolled area will give problems to the surrounding area. In the Kaliurang km.5 street corridor, precisely in Caturtunggal Village, Yogyakarta experienced regional development that is because there are two campuses such as Gadjah Mada University and Yogyakarta State University which have high student attractiveness. This causes a problem impact to traffic in main street, land use change in green open space, and high building density. The concept of a compact city is a concept sustainable development one of the carious sustainable development approaches to solve this problem by considering environmental, economic, and environment aspects. This study aims to examine the characteristics of compact cities in the region Kaliurang km.5 street, as well as providing direction for regional development in realizing it sustainable areas with qualitative descriptive analysis technique.

The aim of this training is to increase the quality of teacher in elementary school of JSIT Semarang through Cooperative Learning training. In the end of this training, the teachers of JSIT?óÔé¼Ôäós Elementary School can understand how the cooperative learning?óÔé¼Ôäós work in the field. They also can practice the method in teaching learning process. Keywords: cooperative learning, quality, teacher Abstrak Pengabdian ini bertujuan untuk meningkatkan kualitas pembelajaran guru-guru SD JSIT Kota Semarang melalui pelatihan Cooperative Learning. Pada akhir pengabdian, para guru-guru SD JSIT tersebut dapat memahami pola pembelajaran Cooperative Learning dan mempraktikkannya dalam kegiatan belajar mengajar di kelas. Kata Kunci: cooperative learning, kualitas, guru

Community service activities for indigenous science and technology telecommunication program is implemented in the form of training with the title BBM Competence Enhancement Loade Mass media Writing Articles for creating Charactear Education Teacher Creative and Productive in Semarang. This activity is done in the form of training that includes delivery of the theory of intelligent technology and the mass media article wraiting practices of mass media in order to provide supplies and motivation for teacher to contribute to Indonesian to contribute their ideas in the word of education to the public through the mass media. The methode used in activity is a lacture, duscussion, and practice. To determine the extent of acceptance and understanding of the participants, as well as the ability of the participants, at the end of training assessment or evaluation. Based on the results of test that have been made know that the participans were very eager to follow the training Wraiting Articles from start to finish. Keywords: competence wraiting, media, teacher

Adolescents are faced with many problems related to reproductive health, so adolescents need to know to be able to face these challenges. However, the knowledge of adolescents in Indonesia is still low, especially in terms of ways to protect themselves against reproductive health risks, such as preventing unwanted pregnancies, sexually transmitted infections, and AIDS. The provision of education needs to be done to address adolescent reproductive health problems. Educational activities related to reproductive health at MTsS Pesantren Thawalib Padang have never been conducted. The students MTsS are the right target group to be able to provide information related to reproductive health. The objective of this service activity is to enhance students' understanding of reproductive health, enabling them to cultivate a positive and responsible approach when addressing the sexual and reproductive changes that transpire within them. The approach employed involves student outreach and the placement of informative posters in their dormitories. The augmentation in students' knowledge was assessed using a questionnaire consisting of 15 questions before and after the activity. Data analysis was conducted through a paired sample t-test, a statistical method that compares the means of two related groups to determine if there is a significant difference between them. After the activity was carried out, there was an increase in the average level of knowledge before (pre-test), 40.63 to 49.76 after (post-test), providing education with a p-value of 0.000. It can be concluded that there is an increase in knowledge after adolescent reproductive health education. It is hoped that students can access reproductive health information from the posters displayed on the dormitory walls.

Naringenin, an abundant flavanon in the peel of citrus fruits is reported to possess anti-proliferative effect in many cancer cells. Herein, we investigated the cytotoxic effect and apoptosis induction of naringenin in combination with doxorubicin on HeLa cells. The cytotoxicity assay of naringenin, doxorubicin, and their combination were carried out by using MTT assay. Cell viability was used as the parameters to evaluate combination effectiveness. Cell cycle distribution was determined by flow cytometry and analyzed using ModFit LT 3.0 program. Apoptosic assay was done by double staining method using Ethidium Bromide-Acridine Orange. Investigation on the expression of Bax and Bcl-2 were determined by immunocytochemistry method. Naringenin and doxorubicin showed cytotoxic effect on HeLa cells with their IC50 values of 195 µM and 1 µM, respectively. Whereas combination of naringenin - doxorubicin showed greater cytotoxicity compared the single treatment of doxorubicin. The strongest cytotoxic activity was observed at a combination of 100 µM naringenin and 0,5 µM doxorubicin. Single treatment of 0,5 µM doxorubicin for 24 hours on HeLa cells induced S-phase arrest while 100 µM naringenin did not affect on HeLa cell cycle. The combination induced S-phase arrest with the increased of sub-G1 phase percentage. In accordance with the flow cytometry results, the double staining apoptosis assay results showed the increase of apoptotic cells. Naringenin, doxorubicin, and their combination also increased the expression of Bax and decreased the expression of Bcl-2. These results concluded that naringenin was a potential co-chemotherapy agent for cervical cancer due to its synergism with doxorubicin. Keywords: co-chemotherapy, naringenin, doxorubicin, HeLa cells, cytotoxicity, cell cycle, apoptosis

Combination of chemotherapeutic agent and chemopreventive agent is being a new approach in cancer treatment. This is aimed at enhancing the effectivity and also reducing drug resistance and adverse side effect of the chemotherapeutic agent. Hesperidin, a citrus flavonoid has reported to reduce the proliferation of many cancer cells. The objectives of this study were to investigate cytotoxic activities, cell cycle modulation and apoptosis induction of hesperidin and its combination with doxorubicin on Hela cell lines. MTT [3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide] assay was used to measure the growth inhibitory effect of hesperidin and its combination with doxorubicin on Hela cells. Cell cycle profile was determined by flowcytometry and the data obtained was analyzed by using ModFit LT 3.0 program. Apoptosis assay was done using double staining method using ethidium-bromide and acridine-orange. Hesperidin inhibited cell growth with IC50 48 μM, while the IC50 of doxorubicin was 1000 nM. Combination of 500 nM doxorubicin and 6 μM hesperidin showed strongest inhibitory effect toward Hela cells. Hesperidin of 24 µM accumulated HeLa cells at G1 phase, but its combination with 500 nM Doxorubicin gave G1 and S phase accumulation at 24 h incubation. Both of Hesperidin and Doxorubicin were capable of inducing apoptosis. In accordance of the apoptotic effect, hesperidin, doxorubicin and their combination decreased the expression Bcl-2 and increased the expression of Bax. According to this result, hesperidin has a potency to be developed as co-chemotherapeutic agent for cervical cancer. Keywords: Cochemotherapy, Hesperidin, Doxorubicin, Hela, MTT assay

Background: Secretomes have been gaining interest in treating several diseases due to their pharmaceutical effects, such as the immunomodulatory effect. This study aimed to determine the immunomodulatory effect of secretomes derived from human umbilical cord mesenchymal stem cells (MSCs) and their safety. Methods: We conducted an in vivo immunomodulatory study using a carbon clearance assay. The safety of single-dose administration of secretome was done using fixed-dose methods of acute toxicity test. Results: The phagocytic index was higher in mice treated with secretome than in untreated mice. The acute toxicity study also showed that the administration of secretome derived from human umbilical cord MSCs did not change the mice’s body weight, physical examination results, organ weight, and gross anatomy examination. Conclusions: This study presents the potential of secretome derived from MSCs as a safe immunomodulatory agent.

Cervical cancer is one of leading causes of cancer death in women in the developing countries. The use of cisplatin as chemotherapy agent in cervical cancer is known to cause side effects and also resistance for long-term uses. One of the strategies to prevent cervical cancer based on combination agents is being developed. Leunca (Solanum nigrum L.) has been revealed to inhibit growth of human cancer cells. Therefore, it can be used in combination with cisplatin to reduce those side effects and prevent the occurrence of cell resistance. Ethanolic extract of Leunca Herb (ELH) and cisplatin were tested their cytotoxic effect on HeLa cervical cancer cell by using MTT assay to determine IC50 value. The combinationss of cisplatin-ELH were tested to determine the combination index (CI value). The IC50 of ELH and cisplatin on HeLa cells were 227 µg/mL and 17 µM. rRespectively. Tthe study of combination resulted that almost all the index combinations were

Honey is one of the honeybee’s’ products which are often used as medicine since a long time ago. Honey has a high antioxidant activity, but studies to investigate its protective effect on ethanol-induced liver injury have not been carried out in Indonesia. The aim of this study was to investigate the protective effect of honey on ethanol-induced rat liver injury. This study done conducted in the Pharmacology and Pathology laboratory in November 2011. This experimental laboratory study used randomized complete design on 25 rats divided into five groups. Group 1: control; group 2: ethanol; group 3, 4 and 5: honey + ethanol. Ethanol 0.01 mL/g body weight was given orally to group 2, 3, 4 and 5 for 14 days. Honey + ethanol groups were given honey at a dose of 0.0018 mL/g body weight, 0.0054 mL/g body weight, and 0.016 mL/g body weight orally at 1.5 hours prior to ethanol administration. Liver samples were taken for histopathological examination. The parameter of liver injury was fatty degeneration. The results showed that ethanol induced fatty degeneration compared to control group. Group 3, 4 and 5 showed significantly decreased fatty degeneration (p

We propose the magnetically separable, green synthesis of Fe3O4/rGO/ZnO using a higher mass ratio of Fe3O4/rGO and varying ZnO concentration to degrade an aqueous solution of Rhodamin B under Fenton reaction and UV light irradiation. Fe3O4 had been synthesized under the coprecipitation method utilizing Moringa oleifera leaf, while rGO had been fabricated by sonicating GO utilizing Amaranthus viridis leaf. Afterward, Fe3O4/rGO was composited under a facile method with a mass ratio of 5:5. And the last, Fe3O4/rGO/ZnO was green-synthesized through precipitation method using Amaranthus viridis leaf with various molarity ratio of Fe3O4/rGO: ZnO equal to 1:1, 1:2, 1:3, 1:4, and 1:5. X-ray diffraction revealed the presence of Fe3O4 and ZnO phases, while Raman spectroscopy confirmed the successful reduction of GO to rGO. The morphological analysis demonstrated that the particles were nearly spherical, nonuniform, and slightly dispersed, with some agglomeration observed on the rGO sheets. Fourier-transform infrared spectroscopy identified metallic functional groups, including Fe–O and Zn–O, at 524–570 cm−1 and 447–462 cm−1, respectively. However, redshift absorption and band gap narrowing were observed as the ZnO concentration increased. Photoluminescence analysis revealed that increased ZnO concentration reduces recombination and improves charge separation efficiency. The vibrating sample magnetometer exhibited soft magnetic properties. The magnetization of Fe3O4/rGO saturated at 21.7 emu/g and diminished as incorporated ZnO. The photodegradation increased with the increase of ZnO concentration, reaching its optimum at 1:5, about 89.9%. Fe3O4/rGO/ZnO demonstrates a possible ecologically friendly photocatalyst for wastewater remediation. The photocatalyst can be reused for up to three cycles with no significant change in its performance. Scavenger evaluation indicates that electrons and holes are the predominant reactive species in the photocatalytic reaction involving Fe₃O₄/rGO/ZnO.

Masalah kesehatan mental menjadi isu global yang sangat umum terjadi, termasuk perubahan suasana hati, perbedaan kepribadian, ketidakmampuan mengatasi masalah, serta mengisolasi diri dari keramaian. Berdasarkan data dari World Health Organization (WHO), gangguan kecemasan dan stres menjadi gangguan mental yang paling sering terjadi dari 970 juta kasus yang dilaporkan sepanjang tahun 2019. Stres telah banyak dikaitkan dengan tidur. Penelitian ini akan mengungkap hubungan kondisi tidur pada manusia dengan tingkat stres yang sedang diderita dengan 5 tingkatan: normal, stres ringan, stres sedang, stres tinggi, stres sangat tinggi. Data yang digunakan merupakan data kontinyu dengan 8 fitur: ‘sr’ (snoring rate), ‘rr’ (respiration rate), ‘t’ (body temperature), ‘lm’ (limb movement), ‘bo’ (blood oxygen), ‘rem’ (rapid eye movement), ‘sh’ (sleeping hours), dan ‘hr’ (heart rate). Setiap fitur memiliki rentang nilai yang tidak sama, sehingga dilakukan normalisasi untuk menyeragamkan rentang tersebut. Hyperparameter tuning dilakukan dengan teknik k-fold cross validation dan model dirancang dengan algoritma klasifikasi Decision Tree serta Random Forest. Hasilnya, 5 fitur: tingkat mendengkur, laju respirasi, pergerakan anggota tubuh termasuk bola mata, serta detak jantung saat tidur berbanding lurus dengan tingkat stres. Semakin tinggi nilai kelima fitur tersebut mengindikasikan tingkat stres yang lebih tinggi. Sedangkan dengan 3 fitur lainnya: suhu tubuh, kadar oksigen, dan waktu tidur memberikan hasil sebaliknya. Dengan kata lain, ketiga nilai tersebut berbanding terbalik dengan tingkat stres yang diderita. Model Decision Tree memiliki akurasi 0,99 dan Random Forest memiliki akurasi 1,0. Hasil penelitian ini diharapkan dapat memberikan insight bagi peneliti lain pada bidang yang sama dan dapat menjadi acuan dalam mendeteksi stres yang sedang diderita. Abstract Stress is often associated with sleep. This research aims to uncover the relationship between human sleep conditions and the level of stress experienced, categorized into five levels: not stressed, very mildly stressed, mildly stressed, highly stressed, and very highly stressed. The data used consists of continuous data with eight features: 'snoring rate' (snoring rate), 'respiration rate' (respiration rate), 'body temperature' (body temperature), 'limb movement' (limb movement), 'blood oxygen' (blood oxygen), 'rapid eye movement' (rapid eye movement), 'sleep hours' (sleep hours), and 'heart rate' (heart rate). Each feature has a different value range, so normalization is performed to standardize these ranges. Hyperparameter tuning is done using k-fold cross-validation, and the model is designed using the Decision Tree and Random Forest classification algorithms. The results show that five features: snoring rate, respiration rate, limb movement including eye movement, and heart rate during sleep are directly proportional to the level of stress. Higher values for these five features indicate higher levels of stress. On the other hand, the other three features: body temperature, blood oxygen level, and sleep hours yield the opposite results. In other words, the values of these three features are inversely proportional to the level of stress experienced. The Decision Tree model has an accuracy of 0.99, and the Random Forest model has an accuracy of 1.0. The results of this research are expected to provide insights for other researchers in the same field and can serve as a reference for detecting ongoing stress.

Abstract Public speaking is the process of speaking to a group of people in a structured, deliberate manner intended to inform, influence, or entertain the listeners. Speaking in front of audience is not an easy task. It needs a good speaking skill. Fear and nervousness become major problem, especially for beginners who have not experienced in public speaking. This also occurs in members of the Muslim Youth Forum Gayamsari (Foksari). Their speaking competence is inadequate. That is why Public Speaking training activity is held. It is expexted to improve their skills. This activity is performed in two stages, namely the delivery of content and practice. Based on the evaluation, the results obtained are very significant. The existence of enthusiastic participants indicates that they are very interested. Besides, there is a seriousness of the participants who attend a series of events organized by the team from beginning to end. Key Words: Skill, Public Speaking, Training Abstrak Berbicara di depan umum adalah proses berbicara kepada sekelompok orang dengan cara terstruktur yang disengaja dimaksudkan untuk menginformasikan, mempengaruhi, atau menghibur pendengar. Berbicara di depan umum bukanlah tugas yang mudah. Perlu keterampilan berbahasa yang baik. Ketakutan dan kegelisahan menjadi masalah besar, terutama bagi pemula yang belum berpengalaman dalam public speaking. Ini juga terjadi pada anggota Youth Forum Muslim Gayamsari (Foksari). Kompetensi berbahasa mereka tidak memadai. Itulah sebabnya kegiatan pelatihan Public Speaking diadakan. Hal ini diharapkan dapat meningkatkan keterampilan mereka. Kegiatan ini dilakukan dalam dua tahap, yaitu pengiriman konten dan praktek. Berdasarkan evaluasi, hasil yang diperoleh sangat signifikan. Adanya peserta antusias menunjukkan bahwa mereka sangat tertarik. Selain itu, ada keseriusan dari para peserta yang menghadiri serangkaian acara yang diselenggarakan oleh tim dari awal sampai akhir. Kata Kunci: Keterampilan, Speaking Training, Public