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The Inga genus comprises approximately 300 species that can be found from Mexico to northern Argentina. In folk medicine, Inga species are used to treat various diseases. The species Inga laurina is widely found in the Brazilian flora; however, there are few studies about its biological activity and chemical composition. The main purpose of this study was to identify and isolate the chemical constituents of Inga laurina barks and to evaluate the antifungal and cytotoxic activities. The total content of phenolics, proanthocyanidins, and flavonoids from the barks of Inga laurina were performed by spectrophotometric methods and the ethyl acetate (EAF) and n-butanol (BF) fractions showed the best results. Eleven compounds were identified in EAF by HPLC-ESI(−)-MS/MS, which showed good antifungal activity with MIC values of 23.4 and 46.8 μg mL−1, evaluated by broth microdilution method. Five new compounds of the genus Inga were isolated for the first time. Three of these compounds were isolated and reported on the literature for the first time: a proanthocyanidin B-type, gallocatechin-(4α→8)-4’-O-methylgallocatechin (XI) and two proanthocyanidins A-type, gallocatechin-(2→O→7,4→8)-4’-O-methylgallocatechin (XII) and gallocatechin-3-O-galloyl-(2→O→7,4→8)-4’-O-methylgallocatechin (XIII). The chemical study of the plant bark showed that this species is rich in phenolic compounds and it has great potential for the discovery of new bioactive compounds.

Made available in DSpace on 2020-12-12T01:54:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-28 Scheelea phalerata Mart. ex Spreng (Arecaceae) is a palm tree found in the Brazilian cerrado. There are no topics related to volatile oils from S. phalerata leaves in the literature. This work determines its chemical composition and evaluates the biological activity under two different seasonal conditions (dry and rainy seasons). The dry essential oil yield was 0.034 ± 0.001% and the rainy essential oil yield was 0.011 ± 0.003%. Both essential oils presented different qualitative and quantitative compositions (99.4 and 98.5%). The main constituents of the dry essential oil were phytol (36.7%), nonadecane (9.7%), linolenic acid (9.1%), (Z)-hex-3-en-1-ol (4.2%), and squalene (4.0%). The main constituents of the rainy essential oil were phytol (26.1%), palmitic acid (18.7%), hexan-1-ol (15.6%), (Z)-hex-3-en-1-ol (9.7%), and oleic acid (4.0%). The antileishmanial activity against promastigotes of Leishmania amazonensis was observed only for the rainy season essential oil (IC50 value of 165.05 ± 33.26 μg mL-1). A molecular docking study showed that alcohols exert a paramount efficacy and that the action of some essential oil compounds may be similar to that of amphotericin B. Still, only the essential oil from the dry season showed moderate antibacterial activity against S. sanguinis (MICs 200-400 μg mL-1). The cytotoxicity against Vero cells was identical (>512) for both essential oils. The novel data here for both chemical characterization and biological activity, in particular, evidence that the action of these compounds is similar to that of amphotericin B, provide valuable information to the drug-discovery field. Nucleus of Research in Natural Products (NuPPeN) Institute of Chemistry Federal University of Uberlândia Campus Santa Mônica, Av. João Naves de ávila, 2121 Institute of Biosciences of Botucatu Department of Microbiology and Immunology Unesp-São Paulo State University, 250 Distrito de Rubião Junior Institute of Biosciences of Botucatu Department of Physics and Biophysics Unesp-São Paulo State University, 250 Distrito de Rubião Junior Nucleus of Research in Sciences and Technology Laboratory of Research in Applied Microbiology (LaPeMA) University of Franca, 201 Parque Universitário Institute of Biomedical Sciences Laboratory of Trypanosomatids Federal University of Uberlândia Campus Umuarama, Av. Pará 1720 Bloco 2B Nucleus of Bioprospecting in Natural Products (NuBiProN) Chemistry Department Federal Institute of the Triângulo Mineiro, 4000 Distrito Industrial I Institute of Biosciences of Botucatu Department of Microbiology and Immunology Unesp-São Paulo State University, 250 Distrito de Rubião Junior Institute of Biosciences of Botucatu Department of Physics and Biophysics Unesp-São Paulo State University, 250 Distrito de Rubião Junior

Aedes aegypti (Diptera: Culicidae) is the main vector of Dengue, Zika and Chikungunya, diseases that have attracted the attention of scientific society and the population in general, due to epidemiological outbreaks and numerous health hazards and the imminent risk of death. Much has been discussed about more efficient forms of control for this mosquito, considering that the chemical control, currently used, has been causing impacts on the environment and the health of the population. Thus, alternative methods have been evaluated. Among them, biological control through products formulated from Bacillus thuringiensis has stood out, as biotechnological advances have allowed to improve and enhance the products already on the market, as well as to develop new bioinsecticides from the entomopathogenic proteins produced by these microorganisms, aiming to make the larvae control more and more effective. To this end, this study aimed to carry out a bibliographic survey on the use of Bacillus thuringiensis as a form of biological control of Aedes aegypti larvae, due to the need to use safer and more effective methods of control for disease vector insects.

P21 is an immunomodulatory protein expressed throughout the life cycle of Trypanosoma cruzi, the etiologic agent of Chagas disease. In vitro and in vivo studies have shown that P21 plays an important role in the invasion of mammalian host cells and establishment of infection in a murine model. P21 functions as a signal transducer, triggering intracellular cascades in host cells and resulting in the remodeling of the actin cytoskeleton and parasite internalization. Furthermore, in vivo studies have shown that P21 inhibits angiogenesis, induces inflammation and fibrosis, and regulates intracellular amastigote replication. In this study, we used the CRISPR/Cas9 system for P21 gene knockout and investigated whether the ablation of P21 results in changes in the phenotypes associated with this protein. Ablation of P21 gene resulted in a lower growth rate of epimastigotes and delayed cell cycle progression, accompanied by accumulation of parasites in G1 phase. However, P21 knockout epimastigotes were viable and able to differentiate into metacyclic trypomastigotes, which are infective to mammalian cells. In comparison with wild-type parasites, P21 knockout cells showed a reduced cell invasion rate, demonstrating the role of this protein in host cell invasion. However, there was a higher number of intracellular amastigotes per cell, suggesting that P21 is a negative regulator of amastigote proliferation in mammalian cells. Here, for the first time, we demonstrated the direct correlation between P21 and the replication of intracellular amastigotes, which underlies the chronicity of T. cruzi infection.

The objective of this work was to characterize the expression of gametophytic self-incompatibility in a Coffea canephora breeding population, to assist in the management and development of new cultivars. For that purpose, 550 in vitro pollinations were carried out among 62 parent plants, of which 27 were from the conilon botanical variety and 35 from the robusta. Thirty-two genotypes compatible with all previously known testers were identified, suggesting the existence of new compatibility groups. From these results, hybridizations were carried out in a complete diallel design with reciprocal crosses to characterize new test plants. Based on the compatibility response with the test plants, the genotypes were clustered into the six following groups: group I, 11 (17.74%) genotypes; group II, 13 (20.97%); group III, 6 (9.68%); group IV, 9 (14.52%); group V, 8 (12.90%); and group VI, 15 (24.19%). The genotypes of the botanical variety robusta show a higher frequency of plants in compatibility group VI and a greater genetic variability, whereas those of the conilon variety have a higher frequency of plants in compatibility group II. The identification of new compatibility groups assists in new management practices that seek to increase the efficiency of pollination by favoring, through natural means, fully compatible crosses. Resumo O objetivo deste trabalho foi caracterizar a expressão de autoincom-patibilidade gametofítica em uma população de melhoramento do cafeeiro Coffea canephora, para fornecer subsídios ao manejo e ao desenvolvimento de novas cultivares. Para tanto, foram realizadas 550 polinizações in vitro entre 62 plantas matrizes, das quais 27 foram da variedade botânica conilon e 35 da robusta. Foram identificados 32 genótipos compatíveis com todos os testadores previamente conhecidos, o que indica a existência de novos grupos de compatibilidade. A partir desses resultados, foram realizadas hibridações em delineamento em dialelo completo com cruzamentos recíprocos para caracterizar novas plantas testadoras. Com base na resposta de compatibilidade com as plantas testadoras, os genótipos foram agrupados nos seguintes seis grupos: grupo I, 11 (17,74%) genótipos; grupo II, 13 (20,97%); grupo III, 6 (9,68%); grupo IV, 9 (14,52%); grupo V, 8 (12,90%); e grupo VI, 15 (24,19%). Os genótipos da variedade botânica robusta apresentam maior frequência de plantas no grupo de compatibilidade VI e maior variabilidade genética, enquanto os da variedade conilon mostram maior frequência de plantas no grupo de compatibilidade II. A identificação de novos grupos de compatibilidade fornece subsídios para novas práticas de manejo que buscam aumentar a eficiência da polinização pelo favorecimento natural de cruzamentos totalmente compatíveis.

The objectives of this study were to optimize in vitro pollination and fluorescence microscopy procedures to characterize the gametophytic self-incompatibility of Coffea canephora genotypes. For that purpose, 115 in vitro pollinations were carried out among 30 parent plants of the Conilon (17) and Robusta (13) botanical varieties. Tester plants of compatibility groups I, II, and III were used as pollen grain donors. On the day of anthesis, pollen grains of the donor genotypes were transferred in vitro to one of the sides of the bifid stigma of each receptor flower. After 30 hours, the development of the pollen tubes was evaluated in fluorescence microscopy. Eleven genotypes (37%) were classified in compatibility Group I, thirteen (43%) in Group II, and six (20%) in Group III. In vitro pollination allowed reduction in the time necessary to diagnose compatibility, without exhibiting contamination caused by the presence of pollen grains of non-donor plants.

P21 is an immunomodulatory protein expressed throughout the life cycle of

The coffee outturn index, understood as the relation between the cherry coffee fruit harvested from the field and its respective processed grains, is an important component of the Coffea canephora bean yield. The aim of this study was to quantify the coffee outturn index of the Amazon Robusta cultivars grown in irrigated and dryland Western Amazon environments. According to the maturation cycle of each clone, washed samples of cherry coffee were collected considering a completely randomized factorial design for characterization of the effects of genotypes, environments, and the genotype × environment (G×E) interaction. The contrasting environments of Porto Velho, RO, and Ouro Preto do Oeste, RO, Brazil, represent most of the coffee growing sites located in Am and Aw climate types, cultivated in Red and Yellow Oxisols, typical of the Western Amazon. The weight reductions through drying exhibited a G×E interaction of the complex type, which is characterized by a change in the ordering of genotypes from one environment to another, whereas the weight reductions through hulling exhibited a G×E interaction predominantly of the simple type. The reduction in weight due to drying was more affected by the environment than reduction in weight after pulping. The clones BRS1216, BRS 3220, and BRS3137 had the highest outturn index estimates and the clones BRS2314, BRS3213, and BRS2336 had the lowest outturn index estimates. The clones BRS2299, BRS3210, BRS3193, and BRS2357 had performance near the mean value of this group of genotypes. Considered together, the genotypes had a mean outturn of 24.41%, with an amplitude from 22.5% to 27.2%.

Amazonian Robustas are ten new clones of high yield potential with characteristics typical of the botanical varieties conilon and robusta. With individual registration, the new cultivars were developed to be grown together with other clones, with flexibility of composition of the crops according to the preference of the producer. Made available in DSpace on 2020-12-08T09:09:39Z (GMT). No. of bitstreams: 1 cpafro-18477.pdf: 280796 bytes, checksum: a6aaba09ddeb6cf541e1fa3fdca8c748 (MD5) Previous issue date: 2020

Class 1 myosins (Myo1s) were the first unconventional myosins identified and humans have eight known Myo1 isoforms. The Myo1 family is involved in the regulation of gene expression, cytoskeletal rearrangements, delivery of proteins to the cell surface, cell migration and spreading. Thus, the important role of Myo1s in different biological processes is evident. In this study, we have investigated the effects of pentachloropseudilin (PClP), a reversible and allosteric potent inhibitor of Myo1s, on angiogenesis. We demonstrated that treatment of cells with PClP promoted a decrease in the number of vessels. The observed inhibition of angiogenesis is likely to be related to the inhibition of cell proliferation, migration and adhesion, as well as to alteration of the actin cytoskeleton pattern, as shown on a PClP-treated HUVEC cell line. Moreover, we also demonstrated that PClP treatment partially prevented the delivery of integrins to the plasma membrane. Finally, we showed that PClP caused DNA strand breaks, which are probably repaired during the cell cycle arrest in the G1 phase. Taken together, our results suggest that Myo1s participate directly in the angiogenesis process.

Made available in DSpace on 2019-10-06T17:17:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01 Termites are eusocial cockroaches, which have received great attention due to their diversity of reproductive strategies. Although these novelties allow new interpretations concerning the mating biology of these insects, studies highlighting the structure of the reproductive system are limited to some termite lineages. Here we provide the first comparative analysis of the reproductive system of a drywood termite, using different aged males of Cryptotermes brevis as models. This species represents an important structural pest in tropical regions, and most aspects of its reproductive biology remain unknown, especially on males. The reproductive apparatus of C. brevis is equipped with paired testes, composed of seven testicular lobes, in which developing spermatozoa are located. The basal portion of the lobes connects to the vasa deferentia and transport spermatozoa to a pair of enlarged chambers, the seminal vesicles. These structures join in a median ejaculatory duct, which opens to the external region through a retractile penis. Spermatozoa were observed in all C. brevis males, exhibiting elongated morphology and measuring about 10 μm in length/4 μm in width. Compared with last-instar nymphs and alates, functional kings showed enlarged testes and seminal vesicles, as well as an intense secretory activity towards the lumen of the latter structures. Histochemical tests evidenced strongly PAS and xylidine Ponceau positive reactions of the secretion only in functional kings, indicating the occurrence of glycoproteins. Thus, we suggest that morphophysiological changes establish during the maturation of the reproductive system in C. brevis. Laboratório de Cupins Departamento de Biologia Instituto de Biociências Universidade Estadual Paulista UNESP, Av. 24A, No. 1515 Laboratório de Cupins Departamento de Biologia Instituto de Biociências Universidade Estadual Paulista UNESP, Av. 24A, No. 1515

Watermelon plants can be naturally infected by several viruses in single or mixed infections, of which the diagnosis is difficult and require specific techniques. This study aims to detect and to verify the presence of Cucumber mosaic virus (CMV), Papaya ringspot virus type W (PRSV-W), Watermelon mosaic virus (WMV), Zucchini lethal chlorosis virus (ZLCV), Zucchini yellow mosaic virus (ZYMV), and the main cucurbit viruses in Brazil using multiplex reverse transcriptase polymerase chain reaction (RT-PCR) assay. Oligonucleotides were designed towards the conserved regions of the virus genomes. In the duplex-PCR, it was possible to detect all the virus combinations, except ZLCV with ZYMV. The amplified product sizes were 644 bp (CMV), 535 bp (WMV-2), 398 bp (PRSV-W), 244 bp (ZLCV), and 214 bp (ZYMV). To test the efficacy of the methodology, we analyzed plants with virus symptoms from four municipalities in the state of Tocantins, located at Brazilian Cerrado. Mixed infections were detected in 80% of samples in all the municipalities. The multiplex RT-PCR assay can be used to detect and differentiate watermelon viruses that affect crops in the state of Tocantins. Key words: Cucumovirus, multiplex RT-PCR, Orthotospovirus, potyvirus, watermelon.

Leishmania (L.) amazonensis is one of the species responsible for the development of cutaneous leishmaniasis in South America. After entering the vertebrate host, L. (L.) amazonensis invades mainly neutrophils, macrophages and dendritic cells. Studies have shown that gal-3 acts as a pattern recognition receptor. However, the role of this protein in the context of L. (L.) amazonensis infection remains unclear. Here, we investigated the impact of gal-3 expression on experimental infection by L. (L.) amazonensis. Our data showed that gal-3 plays a role in controlling parasite invasion, replication and the formation of endocytic vesicles. Moreover, mice with gal-3 deficiency showed an exacerbated inflammatory response. Taken together, our data shed light to a critical role of gal-3 in the host response to infection by L. (L.) amazonensis.

Background: Neural tuberculosis is an infection of the central nervous system caused by the bacterium Mycobacterium tuberculosis. Bearing in mind that neural tuberculosis is the most severe form of extrapulmonary tuberculosis and results in significant morbidity and mortality rates, it is important to analyze its sociodemographic characteristics in Brazil. Objectives: To describe the sociodemographic characteristics of neural tuberculosis cases in Brazil in the period from 2010 to 2020. Design and Setting: Cross-sectional, retrospective, and descriptive study, carried out using data collected from the Notifiable Diseases Information System (SINAN) and the Hospital Information System (SIH / SUS) of confirmed cases and hospitalizations for neural tuberculosis. Methods: Variables used: region, sex, age group, color/race and year of service. Results: In the period from 2010 to 2020, the number of notified cases of meningoencephalic tuberculosis was 7.451, with mean cases per year of 677.36. The Southeast region was the most affected with 43.8% of the total cases, followed by the South and Northeast regions, with 24.1% and 20.1%, respectively. There was a wide predominance in individuals aged 30-39 years (42.8%) and in the age group 40-59 years (35.9%). The male sex was the most affected with 63.3% of the total. Conclusions: The study shows a predominance of cases in the South and Southeast regions, with a significant prevalence of males and age groups of 20- 59. This could be an alert for new prevention and health promotion strategies for the most affected groups.

Background: Stroke is an important cause of death and is a major cause of serious disability in Brazil and in the world. Therefore, it is fundamental to analyze its treatment in the hospital setting in Brazil. Objectives: To describe the progression of the stroke treatment in the hospital setting in Brazil between the years 2010 and 2019 and the outcomes associated to the disease. Design and Setting: Transversal, retrospective and quantitative study, with secondary data collected in the Hospital Information System of SUS (SIH / SUS) between 2010 and 2019, in Brazil. Methods: Data from number of hospitalizations for stroke treatment, mortality rate and average length of hospital stay were collected and analyzed. A comparison has been made between these data over the selected years. Results: The number of hospitalizations for stroke treatment had a 16,83% growth between 2010 and 2019. In comparison, the average length of hospital stay was 7,7 days in 2010 and 7,6 days in 2019. The mortality rate had a reduction of 10,52% between these years. Conclusions: Despite the growth of hospitalization to stroke treatment, there was a reduction in the average length of hospital stay and in the mortality rate associated to the procedure. It is possible to infer an optimization in the stroke lead in intensive care units in Brazil.

Background: Stroke is rare in pediatric patients, but it is of paramount importance due to its serious complications. The study of the incidence of strokes in these patients is important for us to be able to adequate public health policies. Objectives: To evaluate the incidence of strokes in pediatric patients in Brazil. Design and setting: Descriptive, retrospective study, carried out using data from the Hospital Information System (SIH/SUS), from 2011 to 2020. Methods: Variables: brain stroke not specific for the hemorrhagic or ischemic type, mortality, hospitalizations, sex, ethnicity and age group from 0 to 19 years of age. Results: During the period from 2011 from 2020, there were a total of 6912 cases of stroke in the age group from 0 to 19 years; the highest incidence in 2019 (10.59%) and the lowest incidence in 2020 (8.65%.) The age group from 15 to 19 accumulated the greatest number of cases (60.40%). The total mortality rate was 8.12% (561 cases). The highest mortality rate was observed between 15 and 19 years of age (62.03%), and the lowest between 5 and 9 years of age(4.63%). The incidence was slightly higher in males (50.41%). Conclusions: Similar annual stroke rates were identified during the analyzed period, demonstrating the need for interventional actions to reduce its incidence. The non-specification of the hemorrhagic or ischemic types is a limiting factor, since the prevention management is different in each case. There was a higher prevalence, as well as a higher mortality rate, from 15 to 19 years.

Background: Spina bifida is the second leading cause of motor disability in childhood. Therefore, it’s important to know if the therapeutic offer for spina bifida is fulfilling its demand. Objectives: To evaluate the relationship between the number of treatments and the incidence of spina bifida in Brazil, between 2016 and 2019. Design and Setting: Descriptive, retrospective study, with secondary data collected in the Hospital Information System of SUS (SIH / SUS) and the Information System of Live Births (SINASC), between 2016 and 2019, in Brazil. Methods: Variables used: frequency of spina bifida by live births and quantity of treatments. Results: In 2016, the frequency of spina bifida by live births was 746 and the number of procedures for treating the disease was 360, resulting in a 0.48 ratio between the treatment quantity and the affected live births. In 2017, the frequency was 711 and the procedure quantity was 337, with a 0.47 proportion. In 2018, the frequency was 726, the procedure quantity 294, with a 0.4 proportion. In 2019, the frequency decreased to 694, with 288 procedures, and a 0.41 proportion. In total, there were 2,877 live births with spina bifida and 1,300 hospital procedures, with a proportion of 0.45. Conclusions: The study shows a clear disparity between the frequency of spina bifida and the procedure quantity for this condition. In addition, the number of procedures was decreased, without a proportional declination in the disease incidence, indicating a need for greater public investment for such treatments.

Gain from selection is an important criterion in quantifying the efficiency of breeding programs. This study quantified the selection gain estimated under experimental conditions and realized gain achieved in the field, seeking to interpret the efficiency of the Coffea canephora selection. For that purpose, we considered experiments that began in 2004 with directed hybridizations to obtain new hybrid progenies. From a breeding population composed of 288 hybrid individuals, 12 genotypes were selected in experimental conditions from 2005 to 2012, with amplitude in the estimated gains from 127.70 to−19.19%. Those genotypes were evaluated from 2012 to 2018 in clonal tests in four environments of the Western Amazon. The environment that exhibited the greatest correlation between the predicted genetic values and the realized genetic gain observed in the field was the environment of Ouro Preto do Oeste, RO (0.67), the location in which the plants were selected, followed by the environments of Alta FlorestaD´Oeste, RO (0.44), Rio Branco, AC (0.43), and Porto Velho, RO (0.37).Experimental conditions showed that the effect due to dominance deviations was approximately three times greater than the additive effect. Nine clones exhibited higher genetic gains in the experimental conditions and at field, and two clones exhibited lower estimated gains and lower field performance.The clone G17-P7 exhibited high genetic gain under experimental conditions and low field performance. The selection in experimental conditions was positively correlated with plant performance in the field (r=0.55), which allows reduction of the original breeding population to a set of more promising clones to be grown in multiple environments, optimizing time and resources. RESUMO: O ganho com a seleção é um dos critérios mais importantes para avaliar a eficiência de programas de melhoramento de plantas. O objetivo desse trabalho é quantificar a associação entre o ganho de seleção estimado em condições experimentais e o ganho de seleção realizado em campo, buscando quantificar a eficiência do melhoramento do Coffea canephora. Para isso, foram considerados experimentos iniciados com a hibridação direcionada para obtenção de progênies no ano de 2004, a avaliação de testes de progênies em condições experimentais no período de 2005 a 2012, e avaliações em diferentes ambientes da Amazônia Ocidental no período de 2012 a 2018. De uma população composta por 288 indivíduos estruturados em nove progênies de irmãos completos foram selecionados 12 genótipos com amplitude de 127,70 a -19,19% em suas estimativas de ganho com a seleção, para serem avaliados em quatro ambientes da Amazônia Ocidental. O ambiente que apresentou maior correlação entre os valores genéticos preditos e o ganho realizado foi Ouro Preto do Oeste - RO (0,67), local em que as plantas foram selecionadas, seguido pelos ambientes de Alta Floresta D´Oeste - RO (0,44), Rio Branco - AC (0,43) e Porto Velho - RO (0,37). Avaliações em condições experimentais mostraram que os efeitos devido aos desvios de dominância foram aproximadamente três vezes maiores do que os efeitos aditivos. Nove clones apresentaram maiores estimativas de ganho em condições experimentais e nos ensaios de campo, e dois clones apresentaram menores estimativas em ambas condições. O clone G17-P7 apresentou altas estimativas de ganho em condições experimentais e baixo desempenho em campo. A seleção em único ambiente esteve positivamente correlacionada com o desempenho dos cafeeiros em campo (r=0,55), permitindo reduzir a população de melhoramento original em um conjunto de clones de maior potencial agronômico.

Por meio de uma análise bibliométrica, esta pesquisa mapeia a produção científica sobre sucesso em projetos públicos em periódicos disponibilizados na base Web of Science, abrangendo todas as publicações disponibilizadas até o fim de maio de 2019. A amostra compreendeu 248 artigos publicados em 154 periódicos internacionais. Os resultados mostram que a atenção ao tema vem crescendo consideravelmente desde 2013. Porém, a relevância brasileira é muito baixa, se comparada a outros países como Estados Unidos, China, Inglaterra e Austrália, que juntos representam quase 50% da amostra, enquanto o Brasil representa menos de 1%, o que traz um alerta sobre a necessidade da pesquisa acerca do tema. Após análise, os Fatores Críticos de Sucesso (FCS) foram classificados de acordo com Shenhar, Dvir e Levy (2001), sendo os fatores ‘preparação para o futuro’ (39%) e ‘sucesso do negócio’ (27%) os que apareceram com maior frequência. Para a literatura, esta pesquisa contribui com um framework acerca do tema. Para gestores de projetos, esta pesquisa contribui com uma lista dos fatores críticos mais apontados pela literatura, o que pode auxiliá-los a aumentar os índices de sucesso em seus projetos públicos. Abstract Through a bibliometric analysis, this research maps the scientific production on Success in Public Projects in journals made available on the Web of Science database, covering all publications made available until the end of May 2019. The sample comprised 248 articles published in 154 international journals. The results show that attention to the topic has grown considerably since 2013. However, Brazilian relevance is very low, when compared to countries such as the United States, China, England and Australia, which together represent almost 50% of the sample, while Brazil represents less than 1%, which brings an alert of the need for research on the topic. The papers were analyzed and the Critical Success Factors were classified according to Shenhar, Dvir Levy (2001), being the factors: Preparation for the Future (39%) and Business Success (27%) the ones that appeared more frequently. For the literature, this research contributes to a framework on the theme. For project managers, this research contributes to a list of the critical factors most mentioned in the literature, which can help them to increase the success rates in their public projects.

Would STAT1 GOF prevent severe forms of COVID-19?To the Editor:As the world faces the current SARS-CoV-2 pandemic, the scientific community struggles to answer questions about the disease that have not been fully clarified yet. It is well known that individuals with certain chronic diseases are at higher risk of having worse prognosis when infected with this virus. Although the risk of a severe disease is still a matter of conjecture in subjects with Inborn errors of Immunity (IEI), these patients may present more COVID-19 complications than the general population, either by community acquired or nosocomial infection1.In our immunology referral clinic at the Federal University of Sao Paulo (UNIFESP), so far, 16 IEI patients have been diagnosed with COVID-19, with a large spectrum of presentation, ranging from mild disease to severe acute respiratory syndrome and death. One of them, however, had a favorable outcome, with no complications, after being infected with the SARS-CoV-2, despite his several serious comorbidities.We report the case of an 11- year -old boy, diagnosed with Type 1 Diabetes Mellitus at the age of 3 years. At that time, he presented persistent watery diarrhea, 3 to 4 episodes a day. One year later, productive cough, persistent fever, conjunctival hyperemia and generalized lymph node enlargement emerged. Histoplasma sp. was isolated in ocular secretion. Acute Disseminated Histoplasmosis (pulmonary, ocular and ganglionic) was diagnosed and he received Itraconazole for one year.In 2018, at the age of 9 years, we started to follow him at our immunology clinic. He received all vaccines for his age with no adverse events. The family reported recurrent skin infections, repeated pneumonia, mucocutaneous and esophageal candidiasis, and arthralgia. He presented hepatomegaly and failure to thrive. The main diagnostic hypothesis was a disease of immune dysregulation. Screening for inborn errors of immunity and inflammatory lab tests were performed. White blood cell count, immunophenotyping and lymphocyte proliferation, serum immunoglobulins and dihydrorodamine (DHR) assay were in normal range. HIV serologies were negative. Whole exome sequencing was performed and the variant c.1675G> C (de novo variant) appeared in the STAT1 gene (Figure 1)3, in heterozygosis. To date, no data on this genetic variant has been published. This new finding is probably considered pathogenic and, due to the patient’s clinical characteristics, STAT1-GOF is very likely to be the diagnosis of our boy.

Trypanosoma cruzi P21 protein (P21) is a putative secreted and immunomodulatory molecule with potent bioactive properties such as induction of phagocytosis and actin cytoskeleton polymerization. Despite the bioactive properties described so far, the action of P21 on parasite replication in muscle cell lineage or T. cruzi parasitism during acute experimental infection is unclear. We observed that recombinant P21 (rP21) decreased the multiplication of T. cruzi in C2C12 myoblasts, phenomenon associated with greater actin polymerization and IFN-γ and IL-4 higher expression. During experimental infection, lower cardiac nests, inflammatory infiltrate and fibrosis were observed in mice infected and treated with rP21. These results were correlated with large expression of IFN-γ counterbalanced by high levels of IL-10, which was consistent with the lower cardiac tissue injury found in these mice. We have also observed that upon stress, such as that induced by the presence of the IFN-γ cytokine, T. cruzi produced more P21. The effect of P21 in controlling the replication of T. cruzi, may indicate an evolutionary mechanism of survival developed by the parasite. Thus, when subjected to different stress conditions, the protozoan produces more P21, which induces T. cruzi latency in the host organism, enabling the protozoan to evade the host's immune system.

Bacillus thuringiensis serovar israelensis (Bti) is used to control insect vectors of human and animal diseases. In the present study, the toxicity of four strains of Bti, named T0124, T0131, T0137, and T0139, toward Aedes aegypti and Culex quinquefasciatus larvae was analyzed. The T0131 strain showed the highest larvicidal activity against A. aegypti (LC50 = 0.015 µg/ml) and C. quinquefasciatus larvae (LC50 = 0.035 µg/ml) when compared to the other strains. Furthermore, the genomic sequences of the four strains were obtained and compared. These Bti strains had chromosomes sizes of approximately 5.4 Mb with GC contents of ~35% and 5472–5477 putative coding regions. Three small plasmids (5.4, 6.8, and 7.6 kb) and three large plasmids (127, 235, and 359 kb) were found in the extrachromosomal content of all four strains. The SNP-based phylogeny revealed close relationship among isolates from this study and other Bti isolates, and SNPs analysis of the plasmids 127 kb did not reveal any mutations in δ-endotoxins genes. This newly acquired sequence data for these Bti strains may be useful in the search for novel insecticidal toxins to improve existing ones or develop new strategies for the biological control of important insect vectors of human and animal diseases.

Trypanosoma cruzi P21 protein (P21) is a putative secreted and immunomodulatory molecule with potent bioactive properties such as induction of phagocytosis and actin cytoskeleton polymerization. Despite the bioactive properties described so far, the action of P21 on parasite replication in muscle cell lineage or T. cruzi parasitism during acute experimental infection is unclear. We observed that recombinant P21 (rP21) decreased the multiplication of T. cruzi in C2C12 myoblasts, phenomenon associated with greater actin polymerization and IFN-γ and IL-4 higher expression. During experimental infection, lower cardiac nests, inflammatory infiltrate and fibrosis were observed in mice infected and treated with rP21. These results were correlated with large expression of IFN-γ counterbalanced by high levels of IL-10, which was consistent with the lower cardiac tissue injury found in these mice. We have also observed that upon stress, such as that induced by the presence of the IFN-γ cytokine, T. cruzi produced more P21. The effect of P21 in controlling the replication of T. cruzi, may indicate an evolutionary mechanism of survival developed by the parasite. Thus, when subjected to different stress conditions, the protozoan produces more P21, which induces T. cruzi latency in the host organism, enabling the protozoan to evade the host's immune system.

EnglishO desenvolvimento de novas cultivares de Coffea canephora fundamenta-se na caracterizacao do comportamento diferenciado dos clones em diferentes locais de cultivo. O objetivo deste trabalho foi estudar a interacao genotipo x ambientes visando selecionar clones de adaptacao ampla e especifica a diferentes ambientes da Amazonia Ocidental. Doze clones com caracteristicas hibridas das variedades botânicas Conilon e Robusta e quatro clones provenientes de polinizacao aberta tiveram seu desempenho avaliado em comparacao com quatro testemunhas. Os metodos utilizados para quantificar a interacao genotipo x ambientes foram o estimador nao parametrico de Lin & Binns e a dispersao do metodo centroide. A analise de variância indicou que os efeitos de genotipos, de ambiente e da interacao G x A foram significativos (p portuguesThe development of Coffea canephora cultivars is based on the characterization of genotype × environment interaction, which is interpreted to quantify the differential behavior of clones at different cultivation sites. The objective of this research was to study the genotype x environment interaction aiming to select clones of broad and specific adaptation to different environments of the Western Amazon. Twelve clones with hybrid characteristics of the botanical varieties Conilon and Robusta and four open pollinated clones, had their performance evaluated in comparison with four controls. The genotype × environment interaction was interpreted based on the environmental quality index, the nonparametric estimator of Lin and Binns, 1988 and on the dispersion of the centroid method. Effects of the genotypes, environment, and genotype × environment interaction were all significant (p

Purpose – Based on case studies in engineering services companies contracted by Petrobras, which strongly demands the building of dynamic capabilities in its supplier base, we sought to understand how these capabilities are constructed over time. Consequently, we propose new indicators of dynamic capabilities (DCs) and tested their relevance empirically. Design/methodology/approach – Initially, we analysed the relationship of fourteen engineering services companies with Petrobras. Next, we selected two cases to carry out a deeper investigation, evaluating three projects with Petrobras in each case and identifying the creation of dynamic capacities during the projects. Findings – By performing a comprehensive analysis of the case studies, we conclude that through their contracts with Petrobras the engineering services companies develop capabilities that increase the quality and the complexity of the services provided. The four indicators proposed were validated in the field. Originality/value – Empirical studies contribute to understanding the various theoretical interpretations of DCs. In the cases studied, the creation of DCs is linked to new projects with the client. The accumulation of knowledge and learning, when transformed into learning routines and practices, allow companies to develop or reformulate existing skills. Objetivo – Com base em estudos de caso em empresas de serviços de engenharia contratadas pela Petrobras, com forte demanda de construção de capacitações dinâmicas em sua base de fornecedores, procuramos entender como essas capacitações são construídas ao longo do tempo. Consequentemente, propomos novos indicadores de capacitações dinâmicas (CDs) e testamos sua relevância empiricamente. Metodologia – Inicialmente, analisamos a relação de 14 empresas de serviços de engenharia com a Petrobras. Em seguida, selecionamos dois casos para realizar uma investigação mais profunda, avaliando três projetos com a Petrobras em cada caso e identificando a criação de capacitações dinâmicas durante os projetos. Resultados – Ao realizar uma análise abrangente dos estudos de caso, concluímos que, por meio de seus contratos com a Petrobras, as empresas de serviços de engenharia desenvolvem capacitações que aumentam a qualidade e a complexidade dos serviços prestados. Os quatro indicadores propostos foram validados em campo. Contribuições – Estudos empíricos contribuem para a compreensão das várias interpretações teóricas das CDs. Nos casos estudados, a criação de CDs está vinculada a novos projetos com o cliente. O acúmulo de conhecimento e aprendizagem, quando transformado em rotinas e práticas de aprendizagem, permite que as empresas desenvolvam ou reformulem as habilidades existentes. Objetivo – Com base em estudos de caso em empresas de serviços de engenharia contratadas pela Petrobras, com forte demanda de construção de capacitações dinâmicas em sua base de fornecedores, procuramos entender como essas capacitações são construídas ao longo do tempo. Consequentemente, propomos novos indicadores de capacitações dinâmicas (CDs) e testamos sua relevância empiricamente. Metodologia – Inicialmente, analisamos a relação de 14 empresas de serviços de engenharia com a Petrobras. Em seguida, selecionamos dois casos para realizar uma investigação mais profunda, avaliando três projetos com a Petrobras em cada caso e identificando a criação de capacitações dinâmicas durante os projetos. Resultados – Ao realizar uma análise abrangente dos estudos de caso, concluímos que, por meio de seus contratos com a Petrobras, as empresas de serviços de engenharia desenvolvem capacitações que aumentam a qualidade e a complexidade dos serviços prestados. Os quatro indicadores propostos foram validados em campo. Contribuições – Estudos empíricos contribuem para a compreensão das várias interpretações teóricas das CDs. Nos casos estudados, a criação de CDs está vinculada a novos projetos com o cliente. O acúmulo de conhecimento e aprendizagem, quando transformado em rotinas e práticas de aprendizagem, permite que as empresas desenvolvam ou reformulem as habilidades existentes.

Objectives This work aimed to evaluate the antifungal and cytotoxic activity of the EtOH extract and fractions of Banisteriopsis argyrophylla leaves, and to perform the identification of these bioactive metabolites. Methods The EtOAc fraction (EAF) obtained from the ethanolic extract of B. argyrophylla leaves showed better antifungal potential against Candida spp. In this fraction, ten flavonoids have been identified by UHPLC-ESI-MSn. Then, EAF was submitted to column chromatography to give four new fractions (A1–A4). The cytotoxicity was determined against Vero cells. Key findings The EAF showed better antifungal potential against Candida spp. with minimum inhibitory concentrations (MICs) between 31.25 and 93.75 μg/ml. The (–)-catechin (fraction A1) showed a MIC of 2.83 μg/ml against Candida glabrata. Fractions A2, A3 and A4 were rich in quercetins and kaempferols and showed good inhibitory concentrations (5.86–46.87 μg/ml) against C. albicans, C. glabrata and C. tropicalis. Conclusions The EtOH extract, fractions and the isolated (–)-catechin showed lower toxicity to Vero cells than cisplatin, used as a positive control. Thus, the leaves of B. argyrophylla are a promising source of antifungal agents.

Made available in DSpace on 2018-12-11T17:16:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Despite the recent research on Isoptera reproduction strategies, few studies have focused on the morphophysiology of the reproductive system in Neotropical termite species. The classic literature emphasizes the development of the female reproductive apparatus, generating a knowledge gap on the male reproductive system, including its neotenic differentiation and the evolution of the seminal vesicles. The development of the reproductive system in males of Silvestritermes euamignathus was followed from first-instar nymphs until primary kings, with emphasis on the histology of the gonads and seminal vesicles of primary and neotenic kings from field colonies. The maturation of the reproductive system follows post-embryonic development, reaching a peak of development in primary functional kings from field colonies. The testicles are already present in first-instar nymphs and show a progressive development that is completed in primary kings, which are characterized by an enlarged size and greater sperm production. Although spermatogenesis is observed in third-instar nymphs, only in fifth-instar nymphs are spermatozoa present in the testicles and in the seminal vesicles. Spermatozoa are also present in the testicles of neotenic reproductives, derived from different nymphal instars. After the foundation of a new colony and the maturation of the king, there is a significant increase in the reproductive apparatus and the testicular lobes, which mostly occupy the whole posterior abdomen in kings from field colonies. These individuals have a minimum of seven testicular lobes that are completely separated from each other and contain rounded, aflagellated spermatozoa, which are approximately 2–3 μm in diameter. Four-month old kings from laboratory colonies seem to be in a period of reproductive pause, as they display fewer sperm in the seminal vesicles. The seminal vesicles are specialized portions of the vasa deferentia that play a role in storage of spermatozoa. These structures produce a proteinaceous secretion in alates that is not observed in kings. The overall results indicate early maturation of the reproductive system in males of S. euamignathus; furthermore, the reproductive status of the different individuals might be linked to the development of the testicles and the production, storage and release of spermatozoa. The lack of a proteinaceous secretion in the seminal vesicles of kings, in comparison to alates, might be related to the maturation of the individuals after colony establishment. The development of the male reproductive system is consistent with queen physogastry, but in more discreet proportions. Laboratório de Cupins Departamento de Biologia Instituto de Biociências Univ Estadual Paulista UNESP Campus Rio Claro, Avenida 24A, 1515, Bela Vista Instituto de Ciências Agrárias Universidade Federal de Uberlândia UFU Campus Monte Carmelo, Rodovia LMG 746, km 01 Laboratório de Cupins Departamento de Biologia Instituto de Biociências Univ Estadual Paulista UNESP Campus Rio Claro, Avenida 24A, 1515, Bela Vista

Objective The purpose of this clinical study is to demonstrate the effect of classical acupuncture on the Carpal Tunnel Syndrome (CTS) and the relationships that integrate the view of classical acupuncture treatment with current anatomical-physiological knowledge. Design A clinical study was carried out with patients with CTS. Setting The treatments were conducted in a private healthcare practice by a licensed acupuncturist. Participants The five participants presented symptomatology for a period of no less than 6 months, had been diagnosed through electromyography, and additionally showed a positive test for Phalen's maneuver test. All the patients had surgical indications. Interventions Classical Chinese acupuncture was applied during a cycle of treatments. Main measurements The Visual analog scale (VAS) was used to assess the level of discomfort regarding daytime paresthesia, nighttime paresthesia, pain, decrease in strength, and a proposed converted value of pressure pain threshold. Results All the parameters showed significant improvement. The mean difference was negative for pain (− 7,4 points), daytime paresthesia (− 5,2 points), nighttime paresthesia (− 6,2 points), and loss of strength (− 4,6 points), whereas the estimation for the pressure pain threshold was positive (+ 0,683 kgf/cm2). Conclusions In the analysis of the results obtained through the classical acupuncture therapy, all parameters showed an improvement, which suggests and supports evidence of clinical efficacy on the CTS.

The experiment was carried out in 2005 in the municipality of Ouro Preto do Oeste at the experimental station of Embrapa Rondonia. 256 genotypes were evaluated, from nine hybridizations among parents of Kouillou (Cpafro 121, Cpafro 194, Cpafro 199 and Emcapa 03) and Robusta (Robusta 1675, Robusta 2258 and Robusta 640). The experimental design was a randomized complete block design, where each cross was represented by a maximum of 32 plants, distributed in four replications. The spacing used was 3.5 x 1.5 m. The average of six harvests and the yield of hulled coffee was 74.90 bags ha-1. Highlight for progeny is Emcapa 03 x Cpafro 194 with average yield of 97.71 bags ha-1. The estimated heritability observed for yield of coffee benefited, was 78.96%. The evaluation of six production seasons was allowed to verify the reliability of the genetic parameters in each set of crops. It was possible to observe that only the evaluation of the 1st and 2nd crops would be enough to estimate the genetic parameters with efficiency, with a coefficient of determination (R2) of 79.10%. With the inclusion of the 3rd crop, R2 estimates rose to 81.10%. The individual evaluations (per plant) carried out in 2006/2007, 2007/2008 and 2008/2009 crops showed the superiority of the plants from crosses 7, 8 and 9. In relation to resistance of orange rust, most of the genotypes were resistant to disease, and 75% of the genotypes did not present any type of infection symptoms. Among the susceptible genotypes, there was a variation between 1 and 15% in the degree of lesion intensity. Key words: Coffee breeding, amazon, quantitative genetic.

Annona crassiflora Mart., a member of the Annonaceae family found in the Brazilian Savannah, is acknowledged as a plant rich in a variety of isoquinoline alkaloids. The isoquinoline skeleton-derivative alkaloids have been reported as pancreatic lipase (PL) inhibitors. However, the presence of these bioactive compounds in the fruit peel of A. crassiflora remains unclear. This study was conducted to find a bioactive alkaloid not yet described in the fruit peel of A. crassiflora and evaluate its capacity to inhibit the PL activity through in vitro kinetic assay using p -nitrophenyl palmitate as substrate. The ethanolic extract (EtOH) of the peels of A. crassiflora fruits was subjected to liquid−liquid extraction, which yielded an alkaloidal fraction. After a high performance liquid chromatography (HPLC) separation, the purified alkaloid was subjected to structural characterization by high-resolution mass spectrometry with electrospray ionization and electron impact, and nuclear magnetic resonance (NMR) spectroscopy using 1 H, 13 C, correlation spectroscopy (COSY), heteronuclear single-quantum correlation spectroscopy (HSQC), heteronuclear multiple-bond correlation spectroscopy (HMBC) and HMBC-N analyses. The metabolite stephalagine (1,2-methylenedioxy-3-methoxyaporphine), an aporphine alkaloid, was identified. The purified stephalagine was further evaluated as a PL inhibitor. Stephalagine showed high PL inhibitory activity (IC 50 of 8.35 μg mL −1 ) with low cytotoxicity, suggesting a potential anti-obesity agent. This is the first report of the isolation of stephalagine in Annonaceae and its inhibitory effect on pancreatic lipase activity.

Drywood termites of the family Kalotermitidae present a very flexible developmental pattern, in which pseudergates are totipotent and may become reproductives. In this study, three colonies of Cryptotermes brevis headed by neotenic reproductives were used: (i) colony A, with a primary king and a neotenic queen; (ii) colony B, with a primary queen and a neotenic king; and (iii) colony C, with neotenic king and queen. The primary king from colony A and the neotenic king from colony B presented well-developed reproductive systems, with conspicuous testicular lobes and cysts containing spermatozoa also observed in the seminal vesicles. The neotenic queen of colony A and the primary queen of colony B had ovaries with several oocytes in early developmental stages with some of them already vitellogenic and terminal, which suggests egg-laying activity. Both of these queens presented the spermathecae lumen filled with spermatozoa. The queen of colony C had vitellogenic oocytes; however, the oocytes were not terminal and the lumen of spermathecae was devoid of spermatozoa and secretions. The seminal vesicles of colony C neotenic king have no spermatozoa. This king displayed a previtellogenic oocyte among its testicular lobes, which suggested that this individual was an intersex.

Visible Light Communication (VLC) shows up as a trend for short range wireless networks, which comes as a promising breakthrough for industry. However, this technology still faces challenges on the technical side. In particular, the selection of the appropriate circuit driver to fulfill simultaneously both lighting and communication functions is a non-trivial task. This paper reviews driver solutions intended for VLC applications, summarizing published research according to a proposed classification. Additionally, switched mode circuits stand out among the current works, supporting low data rates with higher overall efficiency at greater power levels. On the other hand, linear power amplifiers are the preferred choice for high data rate systems.