220 results on '"Lapierre V"'
Search Results
52. Peripheral stem cell collection (PSCC) in children. It seems to be easy: Review of the problems encountered
- Author
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Beaussier, P.S., primary, Vasselon, S., additional, Brault, Ph., additional, Benhamou, E., additional, Valteau-Couanet, D., additional, Lapierre, V., additional, and Hartmann, O., additional
- Published
- 1997
- Full Text
- View/download PDF
53. Specificities and optimization or peripheral blood stem cell collection in children: Treatment for malignancies can be mobilization
- Author
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Brault, Ph., primary, Beaussier, P.S., additional, Bayle, C., additional, Lapierre, V., additional, Beaujean, F., additional, Antonini, J., additional, Benhamou, E., additional, Valteau-Couanet, D., additional, and Hartmann, O., additional
- Published
- 1997
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- View/download PDF
54. 1201 Consolidation with Busulfan and Melphalan followed by hematopoietic stem-cell transplantation (SCT) in children with poor prognosis Ewing's sarcoma
- Author
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Valteau-Couanet, D., primary, Benhamou, E., additional, Oberlin, O., additional, Couanet, D., additional, Lapierre, V., additional, Beaujean, F., additional, and Hartmann, O., additional
- Published
- 1995
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- View/download PDF
55. Transmission passive d'anticorps anti-HLA granuloagglutinant sans atteinte pulmonaire
- Author
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Charpentier, F., primary, Bierling, P., additional, Lapierre, V., additional, and Duedari, N., additional
- Published
- 1993
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- View/download PDF
56. Traitement empirique des épisodes fébriles survenant chez les patients cancéreux présentant une neutropénie prolongée : essai comparatif ceftazidime seule, ceftazidime + amikacine et ceftazidime + vancomycine
- Author
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Marie, J.P., primary, Pico, J., additional, Lapierre, V., additional, Maulard, C., additional, Pappo, M., additional, Chiche, D., additional, Andremont, A., additional, Lagrange, Ph., additional, Hayat, M., additional, and Zittoun, R., additional
- Published
- 1991
- Full Text
- View/download PDF
57. CD34+ progenitors are reproducibly recovered in thawed umbilical grafts, and positively influence haematopoietic reconstitution after transplantation.
- Author
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Lemarie, C., Esterni, B., Calmels, B., Dazey, B., Lapierre, V., Lecchi, L., Meyer, A., Rea, D., Thuret, I., Chambost, H., Curtillet, C., Chabannon, C., and Michel, G.
- Subjects
BONE marrow transplantation ,CORD blood ,BLOOD platelets ,HEMATOPOIESIS ,BLOOD cells - Abstract
Cord blood (CB) units are increasingly used for allogeneic transplantation. Cell dose, a major factor for CB selection, is evaluated before freezing by each CB bank, using various techniques. This may introduce variability and affect the prediction of cell recovery after thawing, or haematopoietic reconstitution. Forty-two children were transplanted at the same institution with unrelated CB units. All units were thawed and evaluated at the same cell therapy facility, using standard procedures. We investigated: (i) factors that affect cell loss after thawing, and (ii) the importance of CD34
+ cell doses. Prefreeze and post-thaw CD34+ cell doses were statistically correlated, thus suggesting that variability in numeration techniques used by different CB banks does not compromise the biological and clinical value of these figures. CD34+ cell recovery appeared to be correlated with the absolute number of CD34+ cells per frozen bag. Infused CD34+ is the cell dose that better correlates with platelet reconstitution delay; in addition, when using a quartile comparison, haematopoietic recovery appeared to be related with prefreeze and post-thaw CD34+ cell doses. We conclude that enumeration of CD34+ cells in CB units is of biological significance, and may help select CB units and identify patients at risk of delayed recovery.Bone Marrow Transplantation (2007) 39, 453–460. doi:10.1038/sj.bmt.1705618; published online 5 March 2007 [ABSTRACT FROM AUTHOR]- Published
- 2007
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- View/download PDF
58. ARTERIAL AND VENOUS EFFECTS OF VERAPAMIL IN NORMAL VOLUNTEERS.
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THUILLEZ, C., DUHAZE, P., FOURNIER, C., LAPIERRE, V., and GIUDICELLI, J.-F.
- Published
- 1987
- Full Text
- View/download PDF
59. Transfusion-induced immunomodulation following cancer surgery: fact or fiction?
- Author
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Lapierre, Valerie, Auperin, Anne, Tiberghien, Pierre, Lapierre, V, Aupérin, A, and Tiberghien, P
- Subjects
ONCOLOGIC surgery ,BLOOD transfusion - Abstract
Presents a critical review of the literature conducted to see if transfusion of blood products during cancer surgery is capable of inducing immunosuppression, thereby increasing the risk of postoperative infection and or tumor relapse, and if so how this effect can be dealt with. Review conducted on biologic mechanisms; Details pertaining to experimental studies.
- Published
- 1998
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60. Immuno-Hematological and Red Blood Cell Transfusion Practices Before and After Hematopoietic Stem Cell Transplantation: Determination of a Consensus in France
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Lapierre, V., Tiberghien, P., and Kuentz, M.
- Subjects
Blood transfusion -- Innovations ,Hematopoietic stem cells -- Transplantation ,Health ,Innovations - Abstract
Stem Cell Transplantation 'Immuno-Hematological and Red Blood Cell Transfusion Practices Before and After Hematopoietic Stem Cell Transplantation: Determination of a Consensus in France.' V. Lapierre, P. Tiberghien and M. Kuentz. [...]
- Published
- 1999
61. Effect of granulocyte colony-stimulating factor mobilization on phenotypical and functional properties of immune cells
- Author
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Tayebi, H., Kuttler, F., Saas, P., Lienard, A., Petracca, B., Lapierre, V., Ferrand, C., Fest, T., Cahn, J. Y., and Blaise, D.
- Published
- 2001
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62. Intraoperative blood cells salvage in oncologic surgery
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Elias, D., Lapierre, V., and Billard, V.
- Published
- 2000
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63. Erythrocyte transfusions after allogeneic hematopoietic stem cell graft
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Lapierre, V., Kuentz, M., and Tiberghien, P.
- Published
- 1999
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64. Treating severe dry eye syndromes with autologous serum | Traitement des syndromes secs graves par sérum autologue
- Author
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Creuzot-Garcher, C., Lafontaine, P. -O, Brignole, F., Pisella, P. -J, D Athis, P., Bron, A., Lapierre, V., and Christophe Baudouin
65. [What present strategies are helpful in improving transfusion safety in France?]
- Author
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Hervé P, Lapierre V, Pascal MOREL, and Tiberghien P
- Subjects
Quality Control ,Quality Assurance, Health Care ,Research ,Transfusion Reaction ,Blood Component Transfusion ,Blood Donors ,Platelet Transfusion ,Blood Substitutes ,Risk Factors ,Humans ,Blood Transfusion ,France ,Safety ,Biotechnology - Abstract
Transfusion safety rests on measures ensuring that patients are transfused in accordance with the requirements of state-of-the-art scientific knowledge. This strict attitude is part of a quality approach which now applies to all fields of health. Transfusion is historically characterized by its ambivalence: it was the first medical discipline which integrated Quality Assurance concepts, it was also the first which proved unable to respond adequately in the face of uncontrolled risks. Today transfusion must create a system to rapidly: identify any risk, whether emergent or hypothetical; decide which action should be taken; monitor and assess corrective action; study the medico-economic impact of the whole approach. Quality assurance applies to every stage of the transfusion process, from blood donor to labile blood component recipient. This includes blood donor selection and biological control, labile blood component processing, qualification, transport and conditioning, prescription and distribution of blood components and transfused patient follow-up of. Quality controls, "safety locks", must be implemented at every stage to allow early problem detection, thus avoiding potentially dangerous attitudes and guaranteeing transfusion quality all along the process. Medical prescriptions must follow similar rules and meet Good Practice requirements defined by members of the medical and scientific community. A transfusion should not be prescribed unless it is absolutely necessary. In addition to sanitary surveillance, scientific surveillance must also be implemented to help transferring the findings of fundamental research to transfusion activities and continuously improve transfusion safety. INSERM is initiating sociological studies to identify and better understand donors' attitudes leading to risks. More sensitive tests based on nucleic acid amplification should reduce the incidence of residual viral risks. Various viral cell derivative inactivation techniques are being evaluated: the idea is to remove antigens to suppress the risk of post-transfusion alloimmunization. Numerous RD; programs address substitution products. Transfusion safety requires all actors in the field of health being equally involved. Putting together experiences and know-how will continuously strengthen the quality approach adopted in transfusion.
66. Blood transfusion safety: training of the nursing staff using visual aids
- Author
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Lapierre, V., Tramalloni, D., and Oubouzar, N.
- Published
- 1999
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67. Safety and efficacy of alpha-interferon (IFN) for chronic hepatitis C after bone marrow transplantation (BMT)
- Author
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Dowin, C., Leblond, V., Lapierre, V., Gratecos, N., Michallet, M., Faberes, C., Attal, M., Milpied, N., Valla, D., and Vernant, J.P.
- Subjects
Interferon alpha -- Health aspects ,Hepatitis C -- Care and treatment ,Bone marrow -- Transplantation ,Business ,Health care industry - Abstract
According to an abstract submitted by the authors to the 21st Annual Meeting of the European Group for Blood and Marrow Transplantation and the 11th Meeting of the Nurses Group, [...]
- Published
- 1995
68. Transmission of rabies from an organ donor.
- Author
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Jenwitheesuk E, Jackson AC, Lapierre V, Tiberghien P, Srnivasan A, Kuehnert M, and Rupprecht C
- Published
- 2005
69. O2bis-7 Homogénéisation des pratiques transfusionnelles après greffe de cellules souches hématopoïétiques allogéniques provenant du sang périphérique
- Author
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Lapierre, V, Tiberghien, P, Kuentz, M, and pour la Société française de greffe de moelle
- Published
- 1998
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70. Transmission of hepatitis B virus by HBV-negative blood transfusion.
- Author
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Elghouzzi, M H, Couroucé, A M, Magnius, L O, Lunel, F, and Lapierre, V
- Subjects
- *
HEPATITIS B transmission , *DNA analysis , *BLOOD transfusion , *BLOOD banks , *BLOOD donors , *HEPATITIS B , *HEPATITIS viruses , *VIRAL antibodies , *VIRAL antigens - Published
- 1995
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71. People living with HIV display increased anti-apolipoprotein A1 auto-antibodies, inflammation, and kynurenine metabolites: a case-control study.
- Author
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Frias MA, Pagano S, Bararpour N, Sidibé J, Kamau F, Fétaud-Lapierre V, Hudson P, Thomas A, Lecour S, Strijdom H, and Vuilleumier N
- Abstract
Objective: This study aimed to study the relationship between auto-antibodies against apolipoprotein A1 (anti-apoA1 IgG), human immunodeficiency virus (HIV) infection, anti-retroviral therapy (ART), and the tryptophan pathways in HIV-related cardiovascular disease., Design: This case-control study conducted in South Africa consisted of control volunteers ( n = 50), people living with HIV (PLWH) on ART ( n = 50), and untreated PLWH ( n = 44). Cardiovascular risk scores were determined, vascular measures were performed, and an extensive biochemical characterisation (routine, metabolomic, and inflammatory systemic profiles) was performed., Methods: Anti-apoA1 IgG levels were assessed by an in-house ELISA. Inflammatory biomarkers were measured with the Meso Scale Discovery® platform, and kynurenine pathway metabolites were assessed using targeted metabolomic profiling conducted by liquid chromatography-multiple reaction monitoring/mass spectrometry (LC-MRM/MS)., Results: Cardiovascular risk scores and vascular measures exhibited similarities across the three groups, while important differences were observed in systemic inflammatory and tryptophan pathways. Anti-apoA1 IgG seropositivity rates were 15%, 40%, and 70% in control volunteers, PLWH ART-treated, and PLWH ART-naïve, respectively. Circulating anti-apoA1 IgG levels were significantly negatively associated with CD4+ cell counts and positively associated with viremia and pro-inflammatory biomarkers (IFNγ, TNFα, MIPα, ICAM-1, VCAM-1). While circulating anti-apoA1 IgG levels were associated with increased levels of kynurenine in both control volunteers and PLWH, the kynurenine/tryptophan ratio was significantly increased in PLWH ART-treated., Conclusion: HIV infection increases the humoral response against apoA1, which is associated with established HIV severity criteria and kynurenine pathway activation., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (© 2024 Frias, Pagano, Bararpour, Sidibé, Kamau, Fétaud-Lapierre, Hudson, Thomas, Lecour, Strijdom and Vuilleumier.)
- Published
- 2024
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72. A Scoping Review of Alzheimers Disease Hypotheses: An Array of Uni- and Multi-Factorial Theories.
- Author
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Duchesne S, Rousseau LS, Belzile-Marsolais F, Welch LA, Cournoyer B, Arseneau M, Lapierre V, Poulin SM, Potvin O, and Hudon C
- Subjects
- Humans, Brain pathology, Alzheimer Disease etiology, Alzheimer Disease pathology
- Abstract
Background: There is a common agreement that Alzheimers disease (AD) is inherently complex; otherwise, a general disagreement remains on its etiological underpinning, with numerous alternative hypotheses having been proposed., Objective: To perform a scoping review of original manuscripts describing hypotheses and theories of AD published in the past decades., Results: We reviewed 131 original manuscripts that fulfilled our inclusion criteria out of more than 13,807 references extracted from open databases. Each entry was characterized as having a single or multifactorial focus and assigned to one of 15 theoretical groupings. Impact was tracked using open citation tools., Results: Three stages can be discerned in terms of hypotheses generation, with three quarter of studies proposing a hypothesis characterized as being single-focus. The most important theoretical groupings were the Amyloid group, followed by Metabolism and Mitochondrial dysfunction, then Infections and Cerebrovascular. Lately, evidence towards Genetics and especially Gut/Brain interactions came to the fore., Conclusions: When viewed together, these multi-faceted reports reinforce the notion that AD affects multiple sub-cellular, cellular, anatomical, and physiological systems at the same time but at varying degree between individuals. The challenge of providing a comprehensive view of all systems and their interactions remains, alongside ways to manage this inherent complexity.
- Published
- 2024
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- View/download PDF
73. An answer to M Vachon et al.’s article : “Investigating postvention best practices : The Delphi method”. Reflexions on postvention and its evaluation, and the need for an interdisciplinary approach.
- Author
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Lustman M, Lapierre V, and Darrouzes S
- Subjects
- Delphi Technique, Humans, Interdisciplinary Studies, Suicide
- Published
- 2021
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74. Genetic characterization of a unique neuroendocrine transdifferentiation prostate circulating tumor cell-derived eXplant model.
- Author
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Faugeroux V, Pailler E, Oulhen M, Deas O, Brulle-Soumare L, Hervieu C, Marty V, Alexandrova K, Andree KC, Stoecklein NH, Tramalloni D, Cairo S, NgoCamus M, Nicotra C, Terstappen LWMM, Manaresi N, Lapierre V, Fizazi K, Scoazec JY, Loriot Y, Judde JG, and Farace F
- Subjects
- Animals, Benzamides, Cell Line, Tumor, Disease Models, Animal, Drug Resistance, Neoplasm, Gene Expression Regulation, Neoplastic, Homeodomain Proteins metabolism, Humans, Male, Mice, Mice, Inbred NOD, Neoplastic Cells, Circulating drug effects, Nitriles, Phenylthiohydantoin analogs & derivatives, Phenylthiohydantoin pharmacology, Phylogeny, Prostate pathology, Receptors, Androgen genetics, Sequence Alignment, Serine Endopeptidases metabolism, Transcription Factors metabolism, Transcriptome, Tumor Suppressor Protein p53 genetics, Carcinoma, Neuroendocrine genetics, Carcinoma, Neuroendocrine metabolism, Cell Transdifferentiation genetics, Neoplastic Cells, Circulating metabolism, Prostate metabolism, Prostatic Neoplasms genetics, Prostatic Neoplasms metabolism
- Abstract
Transformation of castration-resistant prostate cancer (CRPC) into an aggressive neuroendocrine disease (CRPC-NE) represents a major clinical challenge and experimental models are lacking. A CTC-derived eXplant (CDX) and a CDX-derived cell line are established using circulating tumor cells (CTCs) obtained by diagnostic leukapheresis from a CRPC patient resistant to enzalutamide. The CDX and the derived-cell line conserve 16% of primary tumor (PT) and 56% of CTC mutations, as well as 83% of PT copy-number aberrations including clonal TMPRSS2-ERG fusion and NKX3.1 loss. Both harbor an androgen receptor-null neuroendocrine phenotype, TP53, PTEN and RB1 loss. While PTEN and RB1 loss are acquired in CTCs, evolutionary analysis suggest that a PT subclone harboring TP53 loss is the driver of the metastatic event leading to the CDX. This CDX model provides insights on the sequential acquisition of key drivers of neuroendocrine transdifferentiation and offers a unique tool for effective drug screening in CRPC-NE management.
- Published
- 2020
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- View/download PDF
75. Donor Lymphocyte Infusions After Allogeneic Transplantation: A Single-Center Experience.
- Author
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Kerbage F, Sakr R, Lapierre V, Alexandrova K, Coman T, Leroux S, Lucas N, Pilorge S, Solary E, Bourhis JH, and Castilla-Llorente C
- Subjects
- Acute Disease, Adult, Aged, Female, Humans, Male, Middle Aged, Retrospective Studies, Transplantation, Homologous, Graft vs Host Disease prevention & control, Hematopoietic Stem Cell Transplantation, Leukemia therapy, Lymphocyte Transfusion, Multiple Myeloma therapy, Transplantation Conditioning
- Abstract
Allogeneic hematopoietic cell transplantation (AHCT) represents the only curative therapy for many hematological malignancies. The graft versus leukemia effect, driven by donor T cells, plays a major role in its curative potential. This effect is sometimes very evident when patients with acute myeloid leukemia and myelodysplasia relapse after AHCT and are treated with donor lymphocyte infusions (DLIs). We retrospectively reviewed the charts of 64 patients who received DLI between 2012 and 2017 in our center. The mean age of the patients was 59 years (range, 34-79). Fifty percent were male (n = 32). The mean follow-up time after AHCT was 50.17 months (range, 8-174). The indication for DLI were disease progression, mixed chimerism, minimal residual disease, and other etiologies in 43.8%, 40.7%, 14%, and 1.5% of patients, respectively. The most common diagnosis was acute leukemia, followed by multiple myeloma. Of all patients, 59.4% received a transplant from a related donor, 39% received a transplant from an unrelated donor, and 1.6% received a transplant from a haploidentical donor. Reduced-intensity conditioning AHCT was the most frequent regimen used (53%). DLI was given alone in 79.7% of patients. Prophylactic DLI was given at 30 days after transplantation in patients who received human leukocyte antigen (HLA)-matched related human stem cell transplantation (HSCT) or 45 to 60 days post-transplant in patients receiving haploidentical HSCT or HLA-matched unrelated HSCT. Patients were treated without graft versus host disease (GVHD) prophylaxis. The use of DLI after transplantation remains a feasible procedure with rates of response >60%. Moreover, DLIs are well tolerated with a GVHD rate <10% in our series. We can hypothesize that in our experience the efficacy of this strategy does not rely on the induction of GVHD., (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Published
- 2020
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- View/download PDF
76. Toward a real liquid biopsy in metastatic breast and prostate cancer: Diagnostic LeukApheresis increases CTC yields in a European prospective multicenter study (CTCTrap).
- Author
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Andree KC, Mentink A, Zeune LL, Terstappen LWMM, Stoecklein NH, Neves RP, Driemel C, Lampignano R, Yang L, Neubauer H, Fehm T, Fischer JC, Rossi E, Manicone M, Basso U, Marson P, Zamarchi R, Loriot Y, Lapierre V, Faugeroux V, Oulhen M, Farace F, Fowler G, Sousa Fontes M, Ebbs B, Lambros M, Crespo M, Flohr P, and de Bono JS
- Subjects
- Female, Humans, Leukapheresis methods, Liquid Biopsy methods, Male, Breast Neoplasms blood, Breast Neoplasms pathology, Neoplastic Cells, Circulating pathology, Prostatic Neoplasms, Castration-Resistant blood, Prostatic Neoplasms, Castration-Resistant pathology
- Abstract
Frequently, the number of circulating tumor cells (CTC) isolated in 7.5 mL of blood is too small to reliably determine tumor heterogeneity and to be representative as a "liquid biopsy". In the EU FP7 program CTCTrap, we aimed to validate and optimize the recently introduced Diagnostic LeukApheresis (DLA) to screen liters of blood. Here we present the results obtained from 34 metastatic cancer patients subjected to DLA in the participating institutions. About 7.5 mL blood processed with CellSearch® was used as "gold standard" reference. DLAs were obtained from 22 metastatic prostate and 12 metastatic breast cancer patients at four different institutions without any noticeable side effects. DLA samples were prepared and processed with different analysis techniques. Processing DLA using CellSearch resulted in a 0-32 fold increase in CTC yield compared to processing 7.5 mL blood. Filtration of DLA through 5 μm pores microsieves was accompanied by large CTC losses. Leukocyte depletion of 18 mL followed by CellSearch yielded an increase of the number of CTC but a relative decrease in yield (37%) versus CellSearch DLA. In four out of seven patients with 0 CTC detected in 7.5 mL of blood, CTC were detected in DLA (range 1-4 CTC). The CTC obtained through DLA enables molecular characterization of the tumor. CTC enrichment technologies however still need to be improved to isolate all the CTC present in the DLA., (© 2018 UICC.)
- Published
- 2018
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77. A retrospective, matched paired analysis comparing bendamustine containing BeEAM versus BEAM conditioning regimen: results from a single center experience.
- Author
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Saleh K, Danu A, Koscielny S, Legoupil C, Pilorge S, Castilla-Llorente C, Ghez D, Lazarovici J, Michot JM, Khalife-Saleh N, Lapierre V, Alenxandrova K, Arfi-Rouche J, Bourhis JH, and Ribrag V
- Subjects
- Adult, Aged, Antineoplastic Combined Chemotherapy Protocols adverse effects, Bendamustine Hydrochloride administration & dosage, Bendamustine Hydrochloride adverse effects, Carmustine administration & dosage, Carmustine adverse effects, Case-Control Studies, Cytarabine administration & dosage, Cytarabine adverse effects, Diarrhea chemically induced, Disease-Free Survival, Drug Resistance, Neoplasm, Etoposide administration & dosage, Etoposide adverse effects, Female, Hodgkin Disease pathology, Humans, Lymphoma, Non-Hodgkin pathology, Male, Melphalan administration & dosage, Melphalan adverse effects, Middle Aged, Neoplasm Recurrence, Local, Retrospective Studies, Transplantation, Autologous, Young Adult, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Hodgkin Disease therapy, Lymphoma, Non-Hodgkin therapy, Stem Cell Transplantation methods, Transplantation Conditioning methods
- Abstract
The combination of carmustine, etoposide, aracytin, and melphalan(BEAM) conditioning regimen in autologous stem-cell transplantation (ASCT) is widely used in patients with relapsed/refractory non-Hodgkin lymphoma (NHL) and Hodgkin lymphoma. It is also an option in patients with very-high risk aggressive NHL in first complete remission (CR). Recently, a phase Ib-II feasibility study using bendamustine replacing carmustine (BCNU) was reported. We report herein a safety and efficacy analysis of bendamustine-EAM (BeEAM) with a control BEAM counterpart paired cohort (1/2). One hundred and two patients were analyzed. Overall survival (OS) and progression-free survival (PFS) were not reached and seemed to be comparable between both groups. However, grade III or greater diarrhea was significantly higher in BeEAM patients (44 vs. 15%, p = .002). The median number of days with fever >38 °C was significantly higher in BeEAM group (5.5 vs. 2, p < .001). This case-control study suggests that BeEAM followed by ASCT using bendamustine at 100 mg/m
2 /d is effective but has a different toxicity profile than the BEAM regimen.- Published
- 2018
- Full Text
- View/download PDF
78. Persistence of Yellow Fever Immunization-Induced Antibodies in Allogeneic Hematopoietic Stem Cell Transplant Recipients.
- Author
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Adler M, Lapierre V, Sakr R, Bourhis JH, Gachot B, Castilla-Llorente C, and Wyplosz B
- Subjects
- Humans, Immunization, Immunosuppression Therapy, Hematopoietic Stem Cell Transplantation, Yellow Fever, Yellow Fever Vaccine
- Published
- 2018
- Full Text
- View/download PDF
79. MUB 40 Binds to Lactoferrin and Stands as a Specific Neutrophil Marker.
- Author
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Anderson MC, Chaze T, Coïc YM, Injarabian L, Jonsson F, Lombion N, Selimoglu-Buet D, Souphron J, Ridley C, Vonaesch P, Baron B, Arena ET, Tinevez JY, Nigro G, Nothelfer K, Solary E, Lapierre V, Lazure T, Matondo M, Thornton D, Sansonetti PJ, Baleux F, and Marteyn BS
- Subjects
- Adult, Animals, Biomarkers analysis, Dysentery, Bacillary complications, Dysentery, Bacillary diagnosis, Dysentery, Bacillary immunology, Dysentery, Bacillary microbiology, Female, Guinea Pigs, Humans, Inflammation complications, Inflammation immunology, Inflammation microbiology, Lactoferrin immunology, Mice, Mice, Inbred C57BL, Middle Aged, Neutrophils microbiology, Rabbits, Shigella immunology, Carbocyanines chemistry, Fluorescent Dyes chemistry, Inflammation diagnosis, Lactoferrin analysis, Neutrophils immunology, Peptides chemistry
- Abstract
Neutrophils represent the most abundant immune cells recruited to inflamed tissues. A lack of dedicated tools has hampered their detection and study. We show that a synthesized peptide, MUB
40 , binds to lactoferrin, the most abundant protein stored in neutrophil-specific and tertiary granules. Lactoferrin is specifically produced by neutrophils among other leukocytes, making MUB40 a specific neutrophil marker. Naive mammalian neutrophils (human, guinea pig, mouse, rabbit) were labeled by fluorescent MUB40 conjugates (-Cy5, Dylight405). A peptidase-resistant retro-inverso MUB40 (RI-MUB40 ) was synthesized and its lactoferrin-binding property validated. Neutrophil lactoferrin secretion during in vitro Shigella infection was assessed with RI-MUB40 -Cy5 using live cell microscopy. Systemically administered RI-MUB40 -Cy5 accumulated at sites of inflammation in a mouse arthritis inflammation model in vivo and showed usefulness as a potential tool for inflammation detection using non-invasive imaging. Improving neutrophil detection with the universal and specific MUB40 marker will aid the study of broad ranges of inflammatory diseases., (Copyright © 2018 Elsevier Ltd. All rights reserved.)- Published
- 2018
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- View/download PDF
80. Critical role of the HDAC6-cortactin axis in human megakaryocyte maturation leading to a proplatelet-formation defect.
- Author
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Messaoudi K, Ali A, Ishaq R, Palazzo A, Sliwa D, Bluteau O, Souquère S, Muller D, Diop KM, Rameau P, Lapierre V, Marolleau JP, Matthias P, Godin I, Pierron G, Thomas SG, Watson SP, Droin N, Vainchenker W, Plo I, Raslova H, and Debili N
- Subjects
- Acetylation drug effects, Animals, Blood Platelets cytology, Blood Platelets metabolism, Cell Differentiation genetics, Cells, Cultured, Cortactin genetics, Histone Deacetylase 6 antagonists & inhibitors, Histone Deacetylase 6 genetics, Histone Deacetylase Inhibitors pharmacology, Humans, Hydroxamic Acids pharmacology, Indoles pharmacology, Megakaryocytes cytology, Mice, Knockout, Pyrimidines pharmacology, RNA Interference, Thrombocytopenia genetics, Cortactin metabolism, Histone Deacetylase 6 metabolism, Megakaryocytes metabolism, Thrombocytopenia metabolism
- Abstract
Thrombocytopenia is a major side effect of a new class of anticancer agents that target histone deacetylase (HDAC). Their mechanism is poorly understood. Here, we show that HDAC6 inhibition and genetic knockdown lead to a strong decrease in human proplatelet formation (PPF). Unexpectedly, HDAC6 inhibition-induced tubulin hyperacetylation has no effect on PPF. The PPF decrease induced by HDAC6 inhibition is related to cortactin (CTTN) hyperacetylation associated with actin disorganization inducing important changes in the distribution of megakaryocyte (MK) organelles. CTTN silencing in human MKs phenocopies HDAC6 inactivation and knockdown leads to a strong PPF defect. This is rescued by forced expression of a deacetylated CTTN mimetic. Unexpectedly, unlike human-derived MKs, HDAC6 and CTTN are shown to be dispensable for mouse PPF in vitro and platelet production in vivo. Our results highlight an unexpected function of HDAC6-CTTN axis as a positive regulator of human but not mouse MK maturation.
- Published
- 2017
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81. The use of bone mineral density measured by dual energy X-ray absorptiometry (DXA) and peripheral quantitative computed microtomography in chronic kidney disease.
- Author
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Jannot M, Mac-Way F, Lapierre V, and Lafage-Proust MH
- Subjects
- Fractures, Bone etiology, Humans, Renal Insufficiency, Chronic complications, Risk Assessment methods, Absorptiometry, Photon, Bone Density, Bone and Bones diagnostic imaging, Bone and Bones physiopathology, Fractures, Bone physiopathology, Renal Insufficiency, Chronic physiopathology, X-Ray Microtomography methods
- Abstract
Chronic kidney disease (CKD) is a risk factor for fractures. The current evaluation of fracture risk is based upon the combination of various clinical factors and quantitative imaging of bone. X-ray-based tools were developed to evaluate bone status and predict fracture risk. Dual energy X-ray absorptiometry (DXA) is available worldwide. Longitudinal studies showed that low areal Bone Mineral Density (BMD) measured by DXA predicts fractures in the CKD population as it does in non uremic populations, with good specificity and moderate sensitivity. Peripheral quantitative computed tomography (pQCT) and high resolution pQCT are research tools which measure volumetric BMD at the tibia and radius. They are able to discriminate between the cortical and trabecular envelopes which are differentially affected by renal osteodystrophy. In CKD, a rapid thinning and increased porosity at the cortex is observed which is associated with increased the risk for fracture.
- Published
- 2017
- Full Text
- View/download PDF
82. Engraftment of chronic myelomonocytic leukemia cells in immunocompromised mice supports disease dependency on cytokines.
- Author
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Zhang Y, He L, Selimoglu-Buet D, Jego C, Morabito M, Willekens C, Diop MK, Gonin P, Lapierre V, Droin N, Solary E, and Louache F
- Abstract
Chronic myelomonocytic leukemia (CMML) is a clonal hematopoietic stem cell disorder that typically associates with mutations in epigenetic, splicing, and signaling genes. Genetically modified mouse models only partially recapitulate the disease phenotype, whereas xenotransplantation of CMML cells in immunocompromised mice has been rarely successful so far. Here, CMML CD34
+ cells sorted from patient bone marrow (BM) or peripheral blood (PB) were injected intravenously into NSG (NOD/LtSz-scid IL2rγnull) mice and NSG mice engineered to express human granulo-monocyte colony-stimulating factor, stem cell factor, and interleukin-3 (NSGS mice). Fifteen out of 16 patient samples (94%) successfully engrafted into NSG or NSGS or both mouse strains. The expansion of human cells, predominant in the BM, was also observed in the spleen and the PB and was greatly enhanced in mice producing the 3 human cytokines. Gene mutations identified in engrafted cells were mostly similar to those identified in patient cells before injection. Successful secondary engraftment was obtained in NSGS mice in 3 out of 10 attempts. Thus, primary CMML leukemic cells expand much better in NSGS compared with NSG mice with limited efficacy of secondary transplant., Competing Interests: Conflict-of-interest disclosure: The authors declare no competing financial interests.- Published
- 2017
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83. Activity of nonmuscle myosin II isoforms determines localization at the cleavage furrow of megakaryocytes.
- Author
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Roy A, Lordier L, Mazzi S, Chang Y, Lapierre V, Larghero J, Debili N, Raslova H, and Vainchenker W
- Subjects
- Actins metabolism, Erythrocytes cytology, Humans, Myosin Light Chains metabolism, Phosphorylation, Polymerization, Protein Isoforms metabolism, Protein Transport, Signal Transduction, rho-Associated Kinases metabolism, rhoA GTP-Binding Protein metabolism, Cytokinesis, Megakaryocytes cytology, Megakaryocytes metabolism, Nonmuscle Myosin Type IIA metabolism, Nonmuscle Myosin Type IIB metabolism
- Abstract
Megakaryocyte polyploidy is characterized by cytokinesis failure resulting from defects in contractile forces at the cleavage furrow. Although immature megakaryocytes express 2 nonmuscle myosin II isoforms (MYH9 [NMIIA] and MYH10 [NMIIB]), only NMIIB localizes at the cleavage furrow, and its subsequent absence contributes to polyploidy. In this study, we tried to understand why the abundant NMIIA does not localize at the furrow by focusing on the RhoA/ROCK pathway that has a low activity in polyploid megakaryocytes. We observed that under low RhoA activity, NMII isoforms presented different activity that determined their localization. Inhibition of RhoA/ROCK signaling abolished the localization of NMIIB, whereas constitutively active RhoA induced NMIIA at the cleavage furrow. Thus, although high RhoA activity favored the localization of both the isoforms, only NMIIB could localize at the furrow at low RhoA activity. This was further confirmed in erythroblasts that have a higher basal RhoA activity than megakaryocytes and express both NMIIA and NMIIB at the cleavage furrow. Decreased RhoA activity in erythroblasts abolished localization of NMIIA but not of NMIIB from the furrow. This differential localization was related to differences in actin turnover. Megakaryocytes had a higher actin turnover compared with erythroblasts. Strikingly, inhibition of actin polymerization was found to be sufficient to recapitulate the effects of inhibition of RhoA/ROCK pathway on NMII isoform localization; thus, cytokinesis failure in megakaryocytes is the consequence of both the absence of NMIIB and a low RhoA activity that impairs NMIIA localization at the cleavage furrow through increased actin turnover., (© 2016 by The American Society of Hematology.)
- Published
- 2016
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84. Diagnostic performance of peroxiredoxin 1 to determine time-of-onset of acute cerebral infarction.
- Author
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Richard S, Lapierre V, Girerd N, Bonnerot M, Burkhard PR, Lagerstedt L, Bracard S, Debouverie M, Turck N, and Sanchez JC
- Subjects
- Acute Disease, Adult, Aged, Aged, 80 and over, Area Under Curve, Biomarkers blood, Case-Control Studies, Cerebral Infarction blood, Cerebral Infarction physiopathology, Female, Gene Expression, Humans, Male, Middle Aged, Peroxiredoxins blood, ROC Curve, Time Factors, Cerebral Infarction diagnosis, Cerebral Infarction genetics, Peroxiredoxins genetics
- Abstract
Accurately determining time-of-onset of cerebral infarction is important to clearly identify patients who could benefit from reperfusion therapies. We assessed the kinetics of peroxiredoxin 1 (PRDX1), a protein involved in oxidative stress during the acute phase of ischemia, and its ability to determine stroke onset in a population of patients with known onset of less than 24 hours and in a control group. Median PRDX1 levels were significantly higher in stroke patients compared to controls. PRDX1 levels were also higher from blood samples withdrawn before vs. after 3 hours following stroke onset, and before vs. after 6 hours. ROC analysis with area under the curve (AUC), sensitivity (Se) and specificity (Sp) determined from the Youden index was performed to assess the ability of PRDX1 levels to determine onset. Diagnostic performances of PRDX1 levels were defined by an AUC of 69%, Se of 53% and Sp of 86% for identifying cerebral infarction occurring <3 hours, and an AUC of 68%, Se of 49% and Sp of 88% for cerebral infarction occurring <6 hours. These first results suggest that PRDX1 levels could be the basis of a new method using biomarkers for determining cerebral infarction onset.
- Published
- 2016
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85. Osteoporosis prevention among chronic glucocorticoid users: results from a public health insurance database.
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Trijau S, de Lamotte G, Pradel V, Natali F, Allaria-Lapierre V, Coudert H, Pham T, Sciortino V, and Lafforgue P
- Abstract
Introduction: Long-term glucocorticoid therapy is the leading cause of secondary osteoporosis. The management of glucocorticoid-induced osteoporosis (GIOP) seems to be inadequate in many European countries., Objective: To evaluate the rate of screening and treatment of GIOP., Design: Information was collected from a national public health-insurance database in our geographic area of Provence-Alpes-Côte-d'Azur and in Corsica, from September 2009 through August 2011., Patients: We identified participants aged 15 years and over starting glucocorticoid therapy (≥7.5 mg of prednisone equivalent per day during at least 90 days consecutive). This cohort was compared with an age-matched and sex-matched population that did not receive glucocorticoids., Main Outcome Measures: Bone mass, prescription of bone antiresorptive medication and use of calcium and/or vitamin D treatment., Results: We identified 32 812 patients who were prescribed glucocorticoid therapy, yielding 1% prevalence. Incidence of glucocorticoid therapy was 2.8/1000 inhabitants/year. Males represented 44%, the mean age was 58 years. The median prednisone-equivalent dose was 11 mg/day (IQR 9-18 mg/day). 8% underwent bone mass measurement. Calcium and/or vitamin D, and bisphosphonates were prescribed in 18% and 12%, respectively. Results were lower for the control population: 3% underwent bone mass measurement and 3% received bisphosphonate therapy. The rates of osteodensitometry and treatments were higher in women over 55 years of age than in men and women 55 years of age and younger, and also when glucocorticoid therapy was initiated by a rheumatologist versus other physician specialty., Conclusions: The management of GIOP remains very inadequate, despite the availability of a statutory health insurance system. Targeted interventions are needed to improve the management of GIOP.
- Published
- 2016
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86. Patterns of Methylphenidate Use and Assessment of Its Abuse among the General Population and Individuals with Drug Dependence.
- Author
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Frauger E, Amaslidou D, Spadari M, Allaria-Lapierre V, Braunstein D, Sciortino V, Thirion X, Djezzar S, and Micallef J
- Subjects
- Adolescent, Adult, Databases, Factual, Female, France epidemiology, Humans, Insurance, Health, Reimbursement statistics & numerical data, Male, Young Adult, Epidemiological Monitoring, Methylphenidate adverse effects, Substance Abuse, Intravenous diagnosis, Substance Abuse, Intravenous epidemiology
- Abstract
Purpose: The aim of this study was to describe the extent of methylphenidate (MPH) abuse and characterize its patterns by following several cases involving intravenous administration of crushed MPH tablets., Methods: First, a drug reimbursement database (covering 4 million inhabitants) was explored to assess the magnitude of MPH abuse among the general population, and second, a specific study based on individuals with drug dependence was performed to describe abusers' characteristics (n = 64), patterns of abuse and clinical implications., Results: From 2005 to 2011, the number of patients who were dispensed MPH at least once increased by 166%. The patients with 'deviant' patterns of MPH consumption were mainly male adults with opiate maintenance treatment reimbursements. MPH abusers had precarious living conditions. Half of them consumed MPH daily by intravenous route and reported amphetamine-like effects (cardiovascular events, weight loss, psychiatric adverse events)., Conclusion: Given the increase of MPH use, it is important to warn the scientific community about possible MPH abuse, especially in individuals with drug dependence. This study has facilitated public health intervention and dissemination of information related to MPH abuse among health care professionals at local and national levels., (© 2015 S. Karger AG, Basel.)
- Published
- 2016
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87. Triptans use and overuse: A pharmacoepidemiology study from the French health insurance system database covering 4.1 million people.
- Author
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Braunstein D, Donnet A, Pradel V, Sciortino V, Allaria-Lapierre V, Lantéri-Minet M, and Micallef J
- Subjects
- Adolescent, Adult, Aged, Female, France epidemiology, Humans, Male, Middle Aged, Migraine Disorders economics, Risk Factors, Tryptamines economics, Young Adult, Databases, Factual trends, Drug Utilization trends, Migraine Disorders drug therapy, Migraine Disorders epidemiology, National Health Programs trends, Pharmacoepidemiology trends, Tryptamines therapeutic use
- Abstract
Introduction: The objective of this study was to estimate and to characterize the actual patterns of triptan use and overuse in France using a drug reimbursement database., Methods: We included all people covered by the French General Health Insurance System (GHIS) from the Provence-Alpes-Côte-d'Azur (PACA) and Corsica administrative areas who had at least one dispensed dose of triptans between May 2010 and December 2011. All dispensed doses of triptans, migraine prophylactic treatment and psychotropic medications were extracted from the GHIS database. Triptan overuse was defined as triptan use >20 defined daily doses (DDD) per month on a regular basis for more than three consecutive months. Risk of overuse was assessed using logistic regression adjusted for gender and age., Results: We included 99,540 patients who had at least one prescription of a triptan over the 20 months of the study. Among them, 2243 patients (2.3%) were identified as overusers and received 20.2% of the total DDD prescribed. Twelve percent of overusers and 6.9% of non-overusers were aged more than 65 years (OR: 1.81). Overusers did not have a greater number of prescribers and pharmacists than non-overusers. They were more frequently prescribed a prophylactic medication for migraine treatment (56.8% vs 35.9%, OR: 2.36), benzodiazepines (69.9% vs 54.7%, OR: 1.93) and antidepressants (49.4% vs 30.2%, OR: 2.33)., Conclusions: This work suggests that triptan overuse may be due to insufficient prescriber awareness of appropriate prescribing. The off-label prescription of triptans among the elderly necessitates investigating their cardiovascular risk profile in this sub-group., (© International Headache Society 2015.)
- Published
- 2015
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88. Dendritic cell-derived exosomes as maintenance immunotherapy after first line chemotherapy in NSCLC.
- Author
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Besse B, Charrier M, Lapierre V, Dansin E, Lantz O, Planchard D, Le Chevalier T, Livartoski A, Barlesi F, Laplanche A, Ploix S, Vimond N, Peguillet I, Théry C, Lacroix L, Zoernig I, Dhodapkar K, Dhodapkar M, Viaud S, Soria JC, Reiners KS, Pogge von Strandmann E, Vély F, Rusakiewicz S, Eggermont A, Pitt JM, Zitvogel L, and Chaput N
- Abstract
Dendritic cell-derived exosomes (Dex) are small extracellular vesicles secreted by viable dendritic cells. In the two phase-I trials that we conducted using the first generation of Dex (IFN-γ-free) in end-stage cancer, we reported that Dex exerted natural killer (NK) cell effector functions in patients. A second generation of Dex (IFN-γ-Dex) was manufactured with the aim of boosting NK and T cell immune responses. We carried out a phase II clinical trial testing the clinical benefit of IFN-γ-Dex loaded with MHC class I- and class II-restricted cancer antigens as maintenance immunotherapy after induction chemotherapy in patients bearing inoperable non-small cell lung cancer (NSCLC) without tumor progression. The primary endpoint was to observe at least 50% of patients with progression-free survival (PFS) at 4 mo after chemotherapy cessation. Twenty-two patients received IFN-γ-Dex. One patient exhibited a grade three hepatotoxicity. The median time to progression was 2.2 mo and median overall survival (OS) was 15 mo. Seven patients (32%) experienced stabilization of >4 mo. The primary endpoint was not reached. An increase in NKp30-dependent NK cell functions were evidenced in a fraction of these NSCLC patients presenting with defective NKp30 expression. Importantly, MHC class II expression levels of the final IFN-γ-Dex product correlated with expression levels of the NKp30 ligand BAG6 on Dex, and with NKp30-dependent NK functions, the latter being associated with longer progression-free survival. This phase II trial confirmed the capacity of Dex to boost the NK cell arm of antitumor immunity in patients with advanced NSCLC.
- Published
- 2015
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89. Clinical impact of the NKp30/B7-H6 axis in high-risk neuroblastoma patients.
- Author
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Semeraro M, Rusakiewicz S, Minard-Colin V, Delahaye NF, Enot D, Vély F, Marabelle A, Papoular B, Piperoglou C, Ponzoni M, Perri P, Tchirkov A, Matta J, Lapierre V, Shekarian T, Valsesia-Wittmann S, Commo F, Prada N, Poirier-Colame V, Bressac B, Cotteret S, Brugieres L, Farace F, Chaput N, Kroemer G, Valteau-Couanet D, and Zitvogel L
- Subjects
- Adolescent, Adult, Antineoplastic Agents therapeutic use, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Biomarkers, Tumor, Brain Neoplasms mortality, Cell Line, Tumor, Child, Child, Preschool, Disease-Free Survival, Humans, Infant, Jurkat Cells, Ligands, Neoplasm Metastasis, Neuroblastoma mortality, Phenotype, Prognosis, Prospective Studies, Protein Binding, Risk Factors, Young Adult, B7 Antigens metabolism, Brain Neoplasms metabolism, Gene Expression Regulation, Neoplastic, Natural Cytotoxicity Triggering Receptor 3 metabolism, Neuroblastoma metabolism
- Abstract
The immunosurveillance mechanisms governing high-risk neuroblastoma (HR-NB), a major pediatric malignancy, have been elusive. We identify a potential role for natural killer (NK) cells, in particular the interaction between the NK receptor NKp30 and its ligand, B7-H6, in the metastatic progression and survival of HR-NB after myeloablative multimodal chemotherapy and stem cell transplantation. NB cells expressing the NKp30 ligand B7-H6 stimulated NK cells in an NKp30-dependent manner. Serum concentration of soluble B7-H6 correlated with the down-regulation of NKp30, bone marrow metastases, and chemoresistance, and soluble B7-H6 contained in the serum of HR-NB patients inhibited NK cell functions in vitro. The expression of distinct NKp30 isoforms affecting the polarization of NK cell functions correlated with 10-year event-free survival in three independent cohorts of HR-NB in remission from metastases after induction chemotherapy (n = 196, P < 0.001), adding prognostic value to known risk factors such as N-Myc amplification and age >18 months. We conclude that the interaction between NKp30 and B7-H6 may contribute to the fate of NB patients and that both the expression of NKp30 isoforms on circulating NK cells and the concentration of soluble B7-H6 in the serum may be clinically useful as biomarkers for risk stratification., (Copyright © 2015, American Association for the Advancement of Science.)
- Published
- 2015
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90. What exactly is an unusual sexual fantasy?
- Author
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Joyal CC, Cossette A, and Lapierre V
- Subjects
- Adolescent, Adult, Aged, Female, Humans, Male, Middle Aged, Quebec, Sex Factors, Urban Population, Young Adult, Fantasy, Sexual Behavior psychology, Sexual Dysfunctions, Psychological epidemiology
- Abstract
Introduction: Although several theories and treatment plans use unusual sexual fantasies (SF) as a way to identify deviancy, they seldom describe how the fantasies referred to were determined to be unusual., Aim: The main goal of this study was to determine which SF are rare, unusual, common, or typical from a statistical point of view among a relatively large sample of adults recruited from the general population. A secondary goal was to provide a statistical comparison of the nature and intensity of sexual fantasies for men and women. This study also aims at demonstrating with both quantitative and qualitative analyses that certain fantasies often considered to be unusual are common., Methods: An Internet survey was conducted with 1,516 adults (799 ♀; 717 ♂) who ranked 55 different SF and wrote their own favorite SF. Each SF was rated as statistically rare (2.3% or less), unusual (15.9% or less), common (more than 50%), or typical (more than 84.1% of the sample)., Main Outcome Measures: An extended version of the Wilson's Sex Fantasy Questionnaire with an open question., Results: Only two sexual fantasies were found to be rare for women or men, while nine others were unusual. Thirty sexual fantasies were common for one or both genders, and only five were typical. These results were confirmed with qualitative analyses. Submission and domination themes were not only common for both men and women, but they were also significantly related to each other. Moreover, the presence of a single submissive fantasy was a significant predictor of overall scores for all SF in both genders., Conclusion: Care should be taken before labeling an SF as unusual, let alone deviant. It suggested that the focus should be on the effect of a sexual fantasy rather than its content., (© 2014 International Society for Sexual Medicine.)
- Published
- 2015
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91. [Antipsychotic use in the cohort PACA-Alz in patients with Alzheimer's disease and other dementia in 2010].
- Author
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Bonin-Guillaume S, Martin G, Zafack J, Gentile G, Allaria-Lapierre V, Sciortino V, Thirion X, and Micallef J
- Subjects
- Aged, Aged, 80 and over, Antipsychotic Agents administration & dosage, Cohort Studies, Delivery of Health Care statistics & numerical data, Female, France, Humans, Male, Middle Aged, Psychotropic Drugs administration & dosage, Risperidone administration & dosage, Risperidone therapeutic use, Alzheimer Disease drug therapy, Antipsychotic Agents therapeutic use, Dementia drug therapy, Psychotropic Drugs therapeutic use
- Abstract
Aim: The aim of the study was to identify and to characterize patients with Alzheimer's disease or related dementia describing antipsychotics and other psychotropic expositions., Methods: The study was performed, in 2010, based on Provence-Alpes-Côte d'Azur region (PACA)-Corse Alz cohort included patients with dementia, with chronic condition 'Alzheimer disease or related disease' and/or had at least one delivery of Alzheimer's specific treatment, registered in the General Health Care System. Psychotropic drugs were extracted according anatomical, therapeutical and chemical code. Chronic exposure defined as more than 3 consecutive deliveries., Results: Among 34 696 included patients, 26.9% were men and 68.8% were 80 years old and more. Among them, 26% received at least one antipsychotic medication, with a chronic exposition estimated around 61.3%. Antidepressant and anxiolytic were consumed respectively by 47% and 45.3% of patients. Risperidone was the most used antipsychotic (11.2%). The Health care use (hospitalizations, nurses and physicians visits) was significantly higher among patients with antipsychotics., Conclusion: Antipsychotics use in patients with dementia remains high. The follow up of this regional cohort would be helpful to identify the impact of guidelines on the prescription and the care of patients with dementia., (© 2014 Société Française de Pharmacologie et de Thérapeutique.)
- Published
- 2014
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92. Time-course proteomic analysis of taurocholate-induced necrotizing acute pancreatitis.
- Author
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Fétaud-Lapierre V, Pastor CM, Jorge-Costa M, Hochstrasser DF, Morel DR, Frossard JL, and Lescuyer P
- Subjects
- Acinar Cells pathology, Animals, Antigens, Neoplasm, Biomarkers metabolism, Cholagogues and Choleretics pharmacology, Humans, Lectins, C-Type, Male, Pancreas, Exocrine pathology, Pancreatitis, Acute Necrotizing chemically induced, Pancreatitis, Acute Necrotizing pathology, Pancreatitis-Associated Proteins, Proteomics, Rats, Rats, Sprague-Dawley, Taurocholic Acid pharmacology, Time Factors, Acinar Cells metabolism, Biomarkers, Tumor metabolism, Cholagogues and Choleretics adverse effects, Pancreas, Exocrine metabolism, Pancreatitis, Acute Necrotizing metabolism, Taurocholic Acid adverse effects
- Abstract
Acute pancreatitis is an inflammatory disease of the pancreas, which varies greatly in course and severity. Severe forms are associated with serious local and/or systemic complications, and eventually death. The pathobiology of acute pancreatitis is complex. Animal models have been developed to investigate pathobiological processes and identify factors determining disease course. We performed a time-course proteomic analysis using a rat model of severe necrotizing acute pancreatitis induced by taurocholate perfusion in the pancreatic ducts. Results showed that levels of proteins associated to a given biological process changed in a coordinated fashion after disease onset. It was possible to follow the response of a particular pathobiological process to pancreatitis induction and to compare the course of protein pathways. Proteins involved in acinar cell secretion were found to follow a different kinetics than other cellular processes. After an initial decrease, secretory pathway-associated proteins raised again at 18 h post-induction. This phenomenon coincided with a burst in the expression of pancreatitis-associated protein (REG3A), an acute phase protein produced by the exocrine pancreas, and with the decrease of classical markers of pancreatic injury, suggesting that the expression of proteins associated to the secretory pathway may be a modulating factor of pancreas injury., Biological Significance: Acute pancreatitis (AP) is a complex inflammatory disease, the pathobiology of which is not yet fully understood. Various animal models, relying on different mechanisms of disease induction, have been developed in order to investigate pathobiological processes of AP. In this study, we performed a time-course proteomic analysis to investigate changes of the pancreas proteome occurring in an experimental model of AP induced by perfusion of taurocholate, a bile acid, into the pancreatic duct. This experimental model is characterized by a severe disease with pancreatic necrosis and systemic inflammation. The objectives of this study were to determine the kinetics of functionally related proteins in the early steps of the experimental disease in order to identify protein pathways playing key roles in AP pathobiology and to correlate these data with parameters classically used to assess disease severity. The present work provides for the first time an overview of protein expression in the pancreas during the course of taurocholate-induced necrotizing AP. We believe that correlation of these results with data obtained using proteomic or biochemical approaches in various experimental models of AP will help in highlighting new features, generating hypotheses and constitute therefore a strong and reliable basis for further targeted investigations., (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Published
- 2013
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93. Clinicians' perceptions of factors contributing to complexity and intensity of care of outpatients with traumatic brain injury.
- Author
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Jeyaraj JA, Clendenning A, Bellemare-Lapierre V, Iqbal S, Lemoine MC, Edwards D, and Korner-Bitensky N
- Subjects
- Cognitive Behavioral Therapy, Female, Focus Groups, Humans, Life Style, Male, Personality Assessment, Practice Guidelines as Topic, Qualitative Research, Quality Improvement, Quebec epidemiology, Risk Assessment, Risk Factors, Social Environment, Brain Injuries rehabilitation, Health Personnel, Health Services Needs and Demand, Outpatients, Social Perception
- Abstract
Primary Objective: This study investigated clinicians' perceptions on factors linked to patient complexity in traumatic brain injury (TBI) outpatient rehabilitation., Method: Twelve clinicians from various disciplines, working in TBI outpatient programmes from three rehabilitation institutions in Montreal, Quebec, were recruited using convenience and snowball sampling. Data was collected through focus groups and individual interviews and thematic analysis was used to identify themes., Main Outcomes and Results: Participants identified complexity factors falling under the following themes: sequelae of TBI (cognitive/behavioural/psychological impacts), personal factors (personality traits, pre-medical state, lifestyle and age), patients' environment (architectural, social, language, cultural and financial) and therapeutic relationship (mismatch, misunderstanding and personality clashes). Clinicians also reported facilitators to optimal treatment delivery such as quality of services and working in an interdisciplinary team. Limited time, training and resources were identified as barriers to treatment., Conclusion: A substantial proportion of patients in outpatient TBI programmes seem to follow an atypical evolution and exhibit added complexity. In order to optimize quality of care, clinicians recommended increased community awareness about TBI, increased resources for rehabilitation clinicians and specialized services post-discharge. These findings are insightful for stakeholders; providing a basis for discussions on policy changes that can better meet this population's needs.
- Published
- 2013
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94. Impact of rituximab on stem cell mobilization following ACVBP regimen in poor-risk patients with diffuse large B-cell lymphoma: results from a large cohort of patients.
- Author
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Lefrère F, Bastit-Barrau D, Hequet O, Bourin P, Mathieu-Nafissi S, Bohbot A, Tilly H, Salles G, Fermé C, Lapierre V, Fornecker L, Micléa JM, Isebaert L, Bologna S, Fitoussi O, Mounier N, and Haioun C
- Subjects
- Adolescent, Adult, Antigens, CD34 metabolism, Bleomycin therapeutic use, Cyclophosphamide therapeutic use, Doxorubicin therapeutic use, Humans, Lymphoma, Large B-Cell, Diffuse immunology, Middle Aged, Prednisone therapeutic use, Rituximab, Vindesine therapeutic use, Young Adult, Antibodies, Monoclonal, Murine-Derived therapeutic use, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Hematopoietic Stem Cell Mobilization, Lymphoma, Large B-Cell, Diffuse drug therapy
- Abstract
Background: The ACVBP regimen is an efficient induction regimen for poor-risk patients with diffuse large B-cell lymphoma (DLBCL) before consolidative autologous stem cell transplantation. Adjunction of the monoclonal anti-CD20 antibody rituximab (R-ACVBP) was recently found to be superior to ACVBP alone. This study assessed the impact of rituximab on stem cell mobilization in two similar consecutive groups of patients treated with ACVBP in two prospective, controlled trials., Study Design and Methods: The first trial (LNH-98B-3) involved 137 patients treated with ACVBP alone. In the second trial (LNH-03-3B), 91 patients received an R-ACVBP regimen. Stem cell mobilization was performed after a course of (R)-ACVBP., Results: The median peak numbers of blood CD34+ cell counts recorded before the first apheresis procedure in the ACVBP and R-ACVBP groups were 69×10(6) and 63×10(6) /L, respectively (p=0.55). The median numbers of CD34+ cells collected were 7.1×10(6) and 6.0×10(6) CD34+ cells/kg for the ACVBP and R-ACVBP groups, respectively (p=0.13). The median number of apheresis procedures required for gathering the minimum amount of CD34+ cells (2×10(6) /kg) was the same in the two groups., Conclusion: When compared with ACVBP alone, adjunction of rituximab does not impair stem cell mobilization., (© 2012 American Association of Blood Banks.)
- Published
- 2013
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95. [National and regional market penetration rates of generic's high dosage buprenorphine: its evolution from 2006 to 2008, using reimbursed drug database].
- Author
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Boczek C, Frauger E, Micallef J, Allaria-Lapierre V, Reggio P, and Sciortino V
- Subjects
- Databases, Factual, Databases, Pharmaceutical, Drug Utilization trends, Drugs, Generic, France epidemiology, Humans, Insurance, Health, Reimbursement economics, Insurance, Health, Reimbursement statistics & numerical data, Retrospective Studies, Analgesics, Opioid administration & dosage, Analgesics, Opioid economics, Buprenorphine administration & dosage, Buprenorphine economics
- Abstract
Objective: To assess the national market penetration rate (PR) of generic high-dosage buprenorphine (HDB) in 2008 and its evolution since their marketing (2006), and making a point for each dosage and at regional level., Methods: Retrospective study over data using national and regional health reimbursement database over three years (2006-2008)., Results: In 2008, the generic HDB's national MPR was 31%. The PR for each dosage were 45% for 0.4 mg, 36% for 2 mg and 19% for 8 mg. The (PR) based on Defined Daily Dose (DDD) was 23% in 2008, 15% in 2007 and 4% in 2006. In 2008, at the regional level, disparities were observed in the adjusted penetration rate from 15% in Île de France to 39% in Champagne Ardennes Lorraine., Conclusion: The national PR of generic HDB has increased. There are differences in MPR in terms of dosage and area. However, this PR is still low (in 2008, 82% of the delivered drugs are generics)., (© 2012 Société Française de Pharmacologie et de Thérapeutique.)
- Published
- 2012
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96. Updated technology to produce highly immunogenic dendritic cell-derived exosomes of clinical grade: a critical role of interferon-γ.
- Author
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Viaud S, Ploix S, Lapierre V, Théry C, Commere PH, Tramalloni D, Gorrichon K, Virault-Rocroy P, Tursz T, Lantz O, Zitvogel L, and Chaput N
- Subjects
- Animals, Antigen Presentation, Antigens, Neoplasm immunology, B7-1 Antigen genetics, B7-2 Antigen genetics, CD40 Antigens, CD8-Positive T-Lymphocytes drug effects, CD8-Positive T-Lymphocytes metabolism, Dendritic Cells metabolism, Gene Expression, Humans, Immunoblotting, Intercellular Adhesion Molecule-1 genetics, Lymphocyte Activation, Mice, Mice, Transgenic, CD8-Positive T-Lymphocytes immunology, Cancer Vaccines immunology, Cancer Vaccines therapeutic use, Dendritic Cells immunology, Exosomes immunology, Interferon-gamma immunology
- Abstract
Dendritic cell-derived exosomes (Dex) are nanovesicles bearing major histocompatibility complexes promoting T-cell-dependent antitumor effects in mice. Two phase I clinical trials aimed at vaccinating cancer patients with peptide-pulsed Dex have shown the feasibility and safety of inoculating clinical-grade Dex, but have failed to show their immunizing capacity. These low immunogenic capacities have led us to develop second-generation Dex with enhanced immunostimulatory properties. Here, we show that interferon-γ is a key cytokine conditioning the dendritic cell to induce the expression of CD40, CD80, CD86, and CD54 on Dex, endowing them with direct and potent peptide-dependent CD8(+) T-cell-triggering potential in vitro and in vivo. In this study, we describe the clinical grade process to manufacture large-scale interferon-γ-Dex vaccines and their quality control parameters currently used in a phase II trial.
- Published
- 2011
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97. Proteomic analysis of heat shock-induced protection in acute pancreatitis.
- Author
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Fetaud-Lapierre V, Pastor CM, Farina A, Hochstrasser DF, Frossard JL, and Lescuyer P
- Subjects
- Acute Disease, Animals, Ceruletide, Fever blood, Fever metabolism, Pancreatitis chemically induced, Protective Agents, Rats, Heat-Shock Response, Pancreatitis metabolism, Proteomics methods
- Abstract
Acute pancreatitis is an inflammatory disease of the pancreas, which can result in serious morbidity or death. Acute pancreatitis severity can be reduced in experimental models by preconditioning animals with a short hyperthermia prior to disease induction. Heat shock proteins 27 and 70 are key effectors of this protective effect. In this study, we performed a comparative proteomic analysis using a combination of liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis and isobaric tagging to investigate changes in pancreatic proteins expression that were associated with thermal stress, both in healthy rats and in a model of caerulein-induced pancreatitis. In agreement with previous studies, we observed modulation of heat shock and inflammatory proteins expression in response to heat stress or pancreatitis induction. We also identified numerous other proteins, whose pancreatic level changed following pancreatitis induction, when acute pancreatitis severity was reduced by prior thermal stress, or in healthy rats in response to hyperthermia. Interestingly, we showed that the expression of various proteins associated with the secretory pathway was modified in the different experimental models, suggesting that modulation of this process is involved in the protective effect against pancreatic tissue damage.
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- 2010
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98. Analysis of the pancreatic low molecular weight proteome in an animal model of acute pancreatitis.
- Author
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Lassout O, Pastor CM, Fétaud-Lapierre V, Hochstrasser DF, Frossard JL, and Lescuyer P
- Subjects
- Acute Disease, Amino Acid Sequence, Animals, Ceruletide, Chromatography, Liquid, Disease Models, Animal, Heat-Shock Proteins chemistry, Heat-Shock Proteins metabolism, Immunoblotting, Inflammation, Male, Molecular Sequence Data, Molecular Weight, Pancreatitis chemically induced, Peptides metabolism, Proteins chemistry, Proteins metabolism, Proteome metabolism, Rats, Rats, Sprague-Dawley, Tandem Mass Spectrometry, Pancreatitis metabolism, Peptides chemistry, Proteome chemistry, Proteomics methods
- Abstract
We used a peptidomic approach for the analysis of the low molecular weight proteome in rat pancreatic tissue extracts. The goal was to develop a method that allows identifying endogenous peptides produced in the pancreas in the course of acute pancreatitis. The workflow combines peptides enrichment by centrifugal ultrafiltration, fractionation by isoelectric focusing, and LC-MS/MS analysis without prior enzymatic digestion. The method was assessed on pancreatic extracts from 3 rats with caerulein-induced pancreatitis and 3 healthy controls. A qualitative analysis of the peptide patterns obtained from the different samples was performed to determine the main biological processes associated to the identified peptides. Comparison of peptidomic and immunoblot data for alpha-tubulin, beta-tubulin and coatomer gamma showed that the correlation between the number of identified peptides and the protein abundance was variable. Nevertheless, peptidomic analysis highlighted inflammatory and stress proteins, which peptide pattern was related to acute pancreatitis pathobiology. For these proteins, the higher number of peptides in pancreatitis samples reflected an increase in protein abundance. Moreover, for murinoglobulin-1 or carboxypeptidase B, peptide pattern could be related to protein function. These data suggest that peptidomic analysis is a complementary approach to proteomics for investigating pathobiological processes involved in acute pancreatitis.
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- 2010
- Full Text
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99. [Quality control of defrosted cord blood units: results from an inter-laboratory study].
- Author
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Panterne B, Richard MJ, Sabatini C, Pouthier F, Mouillot L, Bardey D, Boulanger F, Créa S, Dal Cortivo L, Decot V, Fleury-Cappellesso S, Giraud C, Lapierre V, Léauté AG, Le Berre C, Lemarié C, Piard N, Rapatel C, and Rosenzwajg M
- Subjects
- Antigens, CD34 analysis, Blood Cell Count, Blood Preservation methods, Cell Nucleus ultrastructure, Clone Cells cytology, Colony-Forming Units Assay, Female, France, Hematopoietic Stem Cells ultrastructure, Humans, Infant, Newborn, Laboratories, Placenta, Pregnancy, Societies, Medical standards, Blood Preservation standards, Cord Blood Stem Cell Transplantation standards, Cryopreservation standards, Fetal Blood, Quality Control
- Abstract
Purpose: Today, haematopoietic stem cell graft from placental blood concerns more than 15 % of allogeneic grafts. An inter-laboratory study of the quality control of defrosted cord blood units has been coordinated by the French society for cell and tissue bioengineering (SFBCT), with the cord blood bank of Bourgogne Franche-Comté and controlled by the French health products safety agency (Afssaps). The aim of this study is to ensure the inter-laboratory reproducibility of the quality controls practised by the banks during defrosting. The cellular outputs were analyzed according to the defrosting techniques, according to the method used in flow cytometry: single-platform (SP) versus double-platform (DP), or the product nature, i.e. in total blood or miniaturized., Methods: Forty-two units of placental blood (USP), which were out of range were provided for defrosting to 14 participating sites. USP were defrosted and controlled according to the procedures of each bank. Once the USP is defrosted, a part of the product was controlled by the site and the other part by Afssaps. Following controls were carried out: numeration of the total nucleated cells (TNC) and of CD34+ cells (made by a SP method in Afssaps) and functional assay., Results: Concerning TNC, the defrosting sites obtained a cellular output of 94 %+/-28 in day 0 compared with an output of 72 %+/-24 in Afssaps showing a rather good stability of the USP transmitted with an average deviation of 23 %+/-22. The freezing process with or without reduction of volume does not affect this variation. Concerning the numeration of CD34+ cells, the average deviation between the participating sites and Afssaps was 29 %+/-23 compared with 21 %+/-16 for the sites using a SP method against 47 %+/-25 for those using a DP method. The CD34+ outputs are equal to 82 % +/- 60 in day 0 for the participating sites against 52 %+/-20 for Afssaps. For the sites using a DP method, it is stressed that this output is particularly high with a rate of 126 %+/-90 (n=15) whereas it is 62 %+/-20 (n=32) for the sites using a SP method., Conclusion: These results underline a good stability of viable CD34+ cells and a greater reliability of the SP methods for the CD34+ cell numeration for these defrosted USP. Lastly, the results of the functional assay regarding the average clonogenicities (equal to 15 %) reinforce the conclusions on the quality of the defrosted products., (Copyright 2010 Elsevier Masson SAS. All rights reserved.)
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- 2010
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100. Results of cryopreserved parathyroid autografts: a retrospective multicenter study.
- Author
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Borot S, Lapierre V, Carnaille B, Goudet P, and Penfornis A
- Subjects
- Adult, Aged, Bone Diseases epidemiology, Bone Diseases etiology, Bone Diseases prevention & control, Calcium metabolism, Cryopreservation, Female, France, Humans, Male, Middle Aged, Parathyroid Glands physiology, Parathyroidectomy adverse effects, Retrospective Studies, Tissue Banks, Young Adult, Hypoparathyroidism surgery, Parathyroid Glands surgery, Transplantation, Autologous methods
- Abstract
Background: The functionality of cryopreserved parathyroid autotransplantation (CPAT) has been evaluated in few studies, mostly conducted by experienced single-institution centers that have reported different success rates ranging from 17% to 83%. In France, CPAT are rare and their functionality has never been evaluated. Moreover, French tissue banks are facing an accumulation of ungrafted samples. The aim of our work was to evaluate the implantation rate of cryopreserved parathyroid samples and the functionality of CPAT in a multicenter study., Methods: Data from 9 French tissue banks were analyzed. CPAT functionality was defined as fully functional (normal parathyroid hormone [PTH] and calcium levels without treatment), partially functional (normal PTH levels but need for treatment to maintain normocalcemia), and nonfunctional (low PTH levels and need for treatment). For dialyzed patients, CPAT was considered nonfunctional if the PTH level in the nongrafted arm was less than 20 pg/mL, partially functional if the PTH level was between 20 and 50 pg/mL, and fully functional if the PTH level was between 50 and 300 pg/mL., Results: The 9 centers had cryopreserved 1376 samples of parathyroid tissue and only 22 (1.6%) had been autografted in 20 patients (65% renal hyperparathyroidism, 20% multiple endocrine neoplasia type 1, 15% "other") by 12 different surgical teams. The median duration of storage was 11.1 months (range, 0.4-28.5). Only 2 autografts (10%) were fully functional, 2 (10%) were partially functional, and 17 (80%) were nonfunctional at 26 months median follow-up., Conclusion: The reimplantation rate is low, and the functionality of CPAT is less than those published by experienced centers. Logistical and technical problems occurring in less experienced centers are probably the main reasons for nonfunctioning implants. Considering the results of this study, we suggest that cryopreservation of parathyroid glands should be abandoned when not performed in very large experimented centers, that CPAT should be used only for patients with hyperplasic parathyroid tissue, and that tissue samples should be systematically destroyed when patients do not have hypoparathyroidism or after 1 year of storage., (Copyright 2010 Mosby, Inc. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF
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