209 results on '"LI Jin-lan"'
Search Results
52. Imatinib Mesylate Resistance in a Chronic Myeloid Leukemia Patient with a Novel e8a2 BCR-ABL Transcript Variant
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Qin, Ya Zhen, primary, Jiang, Bin, additional, Jiang, Qian, additional, Zhang, Yan, additional, Jiang, Hao, additional, Li, Jin Lan, additional, Zhu, Hong Hu, additional, Li, Ling Di, additional, Liu, Yan Rong, additional, Chen, Shan Shan, additional, and Huang, Xiao Jun, additional
- Published
- 2008
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53. Two Single-Nucleotide Polymorphisms with Linkage Disequilibrium in the Human Programmed Cell Death 5 Gene 5′ Regulatory Region Affect Promoter Activity and the Susceptibility of Chronic Myelogenous Leukemia in Chinese Population
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Ma, Xi, primary, Ruan, Guorui, additional, Wang, Ying, additional, Li, Qiyan, additional, Zhu, Ping, additional, Qin, Ya-Zhen, additional, Li, Jin-Lan, additional, Liu, Yan-Rong, additional, Ma, Dalong, additional, and Zhao, Hongshan, additional
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- 2005
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54. Fabrication of three-dimensional porous scaffolds with controlled filament orientation and large pore size via an improved E-jetting technique.
- Author
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Li, Jin Lan, Cai, Yan Li, Guo, Yi Lin, Fuh, Jerry Ying Hsi, Sun, Jie, Hong, Geok Soon, Lam, Ruey Na, Wong, Yoke San, Wang, Wilson, Tay, Bee Yen, and Thian, Eng San
- Abstract
Biodegradable polymeric scaffolds have been widely used in tissue engineering as a platform for cell proliferation and subsequent tissue regeneration. Conventional microextrusion methods for three-dimensional (3D) scaffold fabrication were limited by their low resolution. Electrospinning, a form of electrohydrodynamic (EHD) printing, is an attractive method due to its capability of fabricating high-resolution scaffolds at the nanometer/micrometer scale level. However, the scaffold was composed of randomly orientated filaments which could not guide the cells in a specific direction. Furthermore, the pores of the electrospun scaffold were small, thus preventing cell infiltration. In this study, an alternative EHD jet printing (E-jetting) technique has been developed and employed to fabricate 3D polycaprolactone (PCL) scaffolds with desired filament orientation and pore size. The effect of PCL solution concentration was evaluated. Results showed that solidified filaments were achieved at concentration >70% (w/v). Uniform filaments of diameter 20 μm were produced via the E-jetting technique, and X-ray diffraction and attenuated total reflectance Fourier transform infrared spectroscopic analyses revealed that there was no physicochemical changes toward PCL. Scaffold with a pore size of 450 μm and porosity level of 92%, was achieved. A preliminary in vitro study illustrated that live chondrocytes were attaching on the outer and inner surfaces of collagen-coated E-jetted PCL scaffolds. E-jetted scaffolds increased chondrocytes extracellular matrix secretion, and newly formed matrices from chondrocytes contributed significantly to the mechanical strength of the scaffolds. All these results suggested that E-jetting is an alternative scaffold fabrication technique, which has the capability to construct 3D scaffolds with aligned filaments and large pore sizes for tissue engineering applications. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2014:102B:651-658. [ABSTRACT FROM AUTHOR]
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- 2014
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55. Applications of Microchip Electrophoresis and Capillary Electrophoresis for Screening FLT3-ITD Gene Mutation in Acute Myeloid Leukemia.
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LENG Xin, LI Ling-Di, LI Jin-Lan, HUANG Xiao-Jun, and RUAN Guo-Rui
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- 2014
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56. Immunophenotypic and Clinical Characteristic Analysis of NPM1 Mutated Acute Myeloid Leukemia with a Normal Karyotype.
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LIU Yan-Rong, LAI Yue-Yun, CHANG Yan, RUAN Guo-Rui, QIN Ya-Zhen, WANG Ya-Zhe, ZHU Hong-Hu, SHI Hong-Xia, JIANG Bin, JIANG Hao, JIANG Qian, HAO Le, and LI Jin-Lan
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- 2013
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57. Clinical Significance of CD34+ CD38+ and CD34+ CD38low/- Subgroups in Bone Marrow of Patients with B Lymploblastic Leukemia.
- Author
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Hao Le, Liu Yan-Rong, Wang Ya-Zhe, Chang Yan, Qin Ya-Zhen, Li Jin-Lan, Li Ling-Di, and Huang Xiao-Jun
- Published
- 2012
58. Abnormal Expression of Programmed Cell Death 5 Gene in Multiple Myeloma Patients.
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BAO Li, RUAN Guo-Rui, LU Xi-Jing, ZHANG Xiao-Hui, LU Jin, NIU Ji-Hong, ZHANG Yao, XIE Min, QIN Ya-Zhen, LI Ling-Di, LI Jin-Lan, LIU Yan-Rong, CHEN Shan-Shan, and HUANG Xiao-Jun
- Published
- 2010
59. Abnormally Lower Expression of Cmtm5 Gene in Bone Marrow Cells from Patients with Multiple Myeloma.
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NIU Ji-Hong, BAO Li, ZHANG Yao, LI Jin-Lan, LI Ling-Di, XIE Min, QIN Ya-Zhen, LAI Yue-Yun, JIANG Qian, SHI Hui-Lin, LIU Yan-Rong, JIANG Bin, CHEN Shan-Shan, HUANG Xiao-Jun, and RUAN Guo-Rui
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- 2010
60. MPL W515L/K mutations in 343 Chinese adults with JAK2V617F mutation-negative chronic myeloproliferative disorders detected by a newly developed RQ-PCR based on TaqMan MGB probes.
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Ruan, Guo-Rui, Jiang, Bin, Li, Ling-Di, Niu, Ji-Hong, Li, Jin-Lan, Xie, Min, Qin, Ya-Zhen, Liu, Yan-Rong, Huang, Xiao-Jun, and Chen, Shan-Shan
- Abstract
Copyright of Hematological Oncology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2010
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61. Comparison between the cultures of the Olympic Games and Naadam Fair.
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LI Jin-lan
- Published
- 2010
62. Follow-up Detection of M-bcr/abl and m-bcr/abl Fusion Transcripts in Chronic Myeloid Leukemia Patients after Allogeneic Hematopoietic Stem Cell Transplantation.
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QIN Ya-Zhen, LIU Yan-Rong, LI Jin-Lan, FU Jia-Yu, CHANG Yan, RUAN Guo-Rui, WANG Hui, QIU Jing-Ying, LU Dao-Pei, and CHEN Shan-Shan
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- 2003
63. Analysis of Immunophenotype of B-Lineage Acute Lymphoblastic Leukemia Cells by 4-Color Flow Cytometry.
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WANG Hui, LIU Yan-Rong, CHEN Shan-Shan, CHANG Yan, QIN Ya-Zhen, LI Jin-Lan, FU Jia-Yu, and RUAN Guo-Rui
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- 2003
64. Immunophenotypic Features of bcr/abl Fusion Transcript-Positive B- Lineage Acute Lymphoblast Leukemia.
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LI Jin-Lan, LIU Yan-Rong, QIN Ya-Zhen, CHANG Yan, FU Jia-Yu, WANG Hui, RUAN Guo-Rui, and CHEN Shan-Shan
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- 2003
65. The Significance of Intracellular IL-lra Expression in the Bone Marrow Cells from Adult Chronic Myelogenous Leukemia.
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RUAN Guo-Rui, CHEN Shan-Shan, WAN Hui, LIU Yan-Rong, CHANG Yan, FU Jia-Yu, LI Jin-Lan, and Qin Ya-Zhen
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- 2003
66. Low Concentrations of ST1571 Enhances β1 Integrin Mediated Inhibitory Effect on Proliferation of Myeloid Progenitors in Ph( + )Chronic Myeloid Leukemia.
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BAI Ren-Kui, CHEN Shan-Shan, LIU Yan-Rong, LI Jin-Lan, and FU Jia-Yu
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- 2001
67. Effect of Autocrine VEGF on Chronic Myeloid Leukemia Cell Line K562.
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RUAN Guo-Rui, LIU Yan-Rong, CHEN Shan-Shan, QIN Ya-Zhen, YU Hong, CHANG Yan, LI Jin-Lan, and FU Jia-Yu
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- 2001
68. Follow up Detection of AML/ETO Fushion Transcripts after Chemotherapy or Bone Marrow Transplantation in Leukemia Patients.
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QIN Ya-Zhen, LIU Yan-Rong, LI Jin-Lan, FU Jia-Yu, CHANG Yan, LU Dao-Pei, GUO Nai-Lan, and CHEN Shan-Shan
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- 2001
69. Expression of Vascular Endothelial Growth Factor in the Bone Marrow Cells from Adult Chronic Myelogenous Leukemia.
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RUAN Guo-Rui, LIU Yan-Rong, CHEN Shan-Shan, LI Jin-Lan, QIN Ya-Zhen, FU Jia-Yu, and BAI Ren-Kui
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- 2001
70. Detection of CSRP2Transcript Levels By Real-Time Quantitative PCR May be a Useful Tool for Monitoring Minimal Residual Disease in B-Cell ALL
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Cao, Lei-Ming, Wu, Li-Xin, Zhao, Ming-Yue, Zhou, Ya-Lan, Li, Jin-Lan, Sun, Qiu-Yu, Jiang, Hao, Qian, Jiang, Xu, Lan-Ping, Zhang, Xiao-Hui, Liu, Kai-Yan, Huang, Xiao-Jun, and Ruan, Guo-Rui
- Abstract
IntroductionCysteine and glycine-rich protein 2 (CSRP2) is gaining increasing attention as a therapeutic target due to its high expression in acute leukemias and its involvement in the development of cancer. However, whether it can be used as a reliable marker for minimal residual disease (MRD) remains unknown.
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- 2021
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71. Evaluating the Feasibility of Nucleated Cells Isolated by Lysis Method for Real-time Quantitative RT-PCR.
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LI Ling-Di, QIN Ya-Zhen, LI Jin-Lan, and LIU Yan-Rong
- Published
- 2011
72. Immunophenotypic Characteristics of Multiple Myeloma Cells.
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LI Jin-Lan, LIU Yan-Rong, CHANG Yah, FU Jia-Yu, and CHEN Shah-Shah
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- 2002
73. Dead/H-Box Helicase 11 (DDX11) Mutations Correlate with Increased Relapse Risk in Persons with Acute Myeloid Leukaemia and Promote Proliferation and Survival of Human AML Cells in Vitroand in Immune Deficient Mice
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Zhou, Ya-Lan, Wu, Li-Xin, Gale, Robert Peter, Wang, Zi-Long, Li, Jin-Lan, Jiang, Hao, Jiang, Qian, Jiang, Bin, Cao, Shan-Bo, Sun, Ying, Lou, Feng, Wang, Chengcheng, Liu, Yan-Rong, Wang, Yu, Chang, Ying-Jun, Xu, Lanping, Zhang, Xiaohui, Liu, Kaiyan, Ruan, Guorui, and Huang, Xiaojun
- Abstract
Background About one-half of persons with acute myeloid leukemia have normal cytogenetics but have diverse outcomes which might be explained, at least in part, by specific mutations. We focused on DDX11,the budding yeast ortholog of ChlR1, which encodes an ATP-dependent RNA- and DNA-helicase involved in diverse cell processes such as sister chromatid exchange cohesions and implicated in other cancers and which is associated with telomere shortening.
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- 2019
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74. Genomic Landscape and Risk-Stratification for De NovoAcute Myeloid Leukemia with Normal Cytogenetics and No NPM1or FLT3-itd Mutation
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Zhou, Ya-Lan, Wu, Li-Xin, Gale, Robert Peter, Wang, Zi-Long, Li, Jin-Lan, Jiang, Hao, Jiang, Qian, Jiang, Bin, Cao, Shan-Bo, Lou, Feng, Sun, Ying, Wang, Chengcheng, Li, Ting, Liu, Yan-Rong, Wang, Yu, Chang, Ying-Jun, Xu, Lan-Ping, Zhang, Xiaohui, Liu, Kaiyan, Ruan, Guorui, and Huang, Xiaojun
- Abstract
Introduction-About 25% of persons with new-diagnosed acute myeloid leukemia (AML) have normal cytogenetics and no NPM1or FLT3-ITDmutation. The prognosis and best therapy of these persons is controversial.
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- 2019
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75. A new N-nitroso compound, N-(2-methylpropyl)-N-(1-methylacetonyl)nitrosamine in moldy millet and wheat flour
- Author
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Ji, Chuan, primary, Xu, Zhi Xiang, additional, Li, Ming Xin, additional, Li, Guo Yu, additional, and Li, Jin Lan, additional
- Published
- 1986
- Full Text
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76. Mutation Topology of Adult B-Cell Acute Lymphoblastic Leukaemia
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Wu, Li-Xin, Lu, Run-Qing, Liu, Kai-Yan, Gale, Robert Peter, Zheng, Qi, Zhou, Jiao, Li, Jin-Lan, Wang, Shu-Juan, Jiang, Hao, Jiang, Qian, Jiang, Bin, Zhang, Xiao-Hui, Xu, Lan-Ping, Wang, Yue, Song, Cheng-Li, Qin, Ya-Zhen, Liu, Yan-Rong, Huang, Xiao-Jun, and Ruan, Guo-Rui
- Abstract
Background:Molecular analyses of risk cohorts in adults with B-cell acute lymphoblastic leukaemia (ALL) has lagged behind progress in children with B-cell ALL. Two widely recognized prognostic variables are mutations in BCRABL1and in IKZF1with mutation frequencies of about 25 and 50 percent in unselected adults.
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- 2017
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77. Which method better evaluates the molecular response in newly diagnosed chronic phase chronic myeloid leukemia patients with imatinib treatment, BCR-ABLIS or log reduction from the baseline level?
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Qin, Ya-Zhen, Jiang, Qian, Jiang, Hao, Li, Jin-Lan, Li, Ling-Di, Zhu, Hong-Hu, Lai, Yue-Yun, Lu, Xi-Jing, Liu, Yan-Rong, Jiang, Bin, and Huang, Xiao-Jun
- Subjects
- *
CHRONIC myeloid leukemia , *TREATMENT of chronic myeloid leukemia , *IMATINIB , *PROTEIN-tyrosine kinase inhibitors , *MESSENGER RNA , *MMR vaccines , *PATIENTS - Abstract
Abstract: The molecular response of chronic myeloid leukemia (CML) patients to tyrosine kinase inhibitor treatment can be evaluated either by BCR-ABL mRNA levels on international scale (IS) or by log reduction from the baseline level of the laboratory. Both methods were compared in 248 newly diagnosed chronic phase CML patients treated with imatinib. The major molecular responses (MMR) obtained by both methods predict progression-free survival (PFS, all P <0.0001). Thirty-six patients, who were identified as MMR patients by the IS method but as non-MMR patients by the log reduction method, had the same PFS as MMR patients identified by both methods. The molecular responses of patients at 3 and 6 months, as evaluated by the two methods, have similar predictive values on their cytogenetic responses at 12 months and on their molecular responses at 18 months. Both ≤10%IS and ≥1 log reduction at 3 months and ≤1%IS at 6 months were significantly associated with PFS (P =0.0011, 0.0090, and 0.0064). The percentages of patients with BCR-ABLIS of ≤1%, >1–10%, and of >10% at 3 months and 6 months in the German CML Study IV were similar with those with corresponding BCR-ABLIS in our center, but was significantly different with those evaluated by the log reduction method. Therefore, the molecular response evaluated by BCR-ABLIS has similar trends in PFS and in response prediction, but can better differentiate patients than that by the log reduction method. Furthermore, the IS method allows comparison among molecular response results from different laboratories. [Copyright &y& Elsevier]
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- 2013
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78. [Applications of microchip electrophoresis and capillary electrophoresis for screening FLT3-ITD gene mutation in acute myeloid leukemia].
- Author
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Leng X, Li LD, Li JL, Huang XJ, and Ruan GR
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Female, Humans, Leukemia, Myeloid, Acute diagnosis, Male, Middle Aged, Mutation, Young Adult, Electrophoresis, Capillary, Electrophoresis, Microchip, Leukemia, Myeloid, Acute genetics, fms-Like Tyrosine Kinase 3 genetics
- Abstract
The purpose of the present study was to compare the reliability of microchip electrophoresis and capillary electrophoresis for screening FLT3-ITD gene mutation in acute myeloid leukemia. The FLT3-ITD mutation in the genomic DNA samples from 214 untreated AML patients were separately detected by PCR-microchip electrophoresis and PCR-capillary electrophoresis, then the DNA direct sequencing analysis was carried out. The results from PCR-microchip electrophoresis showed that there were 151 FLT3-ITD mutation negative, 58 FLT3-ITD mutation positive (58/214, 27.1%) and 5 FLT3-ITD mutation doubtful positive (5/214, 2.3%), while the outcomes from PCR-capillary electrophoresis displayed that there were 147 FLT3-ITD mutation negative and 67 FLT3-ITD mutation positive (67/214, 31.3%) without doubtful positive. In the 67 FLT3-ITD mutation positive samples detected by using PCR-capillary electrophoresis, 4 samples were detected as the negative while 5 samples were measured as the doubtful positive by using PCR-microchip electrophoresis. The followed sequencing analysis demonstrated that the above 9 samples were all FLT3-ITD mutation positive, indicating that PCR-capillary electrophoresis was more accurate and sensitive in screening the FLT3-ITD mutation, although statistic analysis showed that there were no significant differences in the detected results between PCR-microchip electrophoresis and PCR-capillary electrophoresis groups (Pearson Chi-squared Test, P > 0.05). It is concluded that both PCR-microchip electrophoresis and PCR-capillary electrophoresis were convenient and fast for screening FLT3-ITD mutation, but the accuracy of PCR-microchip electrophoresis awaits further improvement.
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- 2014
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79. [Immunophenotypic and clinical characteristic analysis of NPM1 mutated acute myeloid leukemia with a normal karyotype].
- Author
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Liu YR, Lai YY, Chang Y, Ruan GR, Qin YZ, Wang YZ, Zhu HH, Shi HX, Jiang B, Jiang H, Jiang Q, Hao L, and Li JL
- Subjects
- Adolescent, Adult, Aged, Child, Child, Preschool, Female, Flow Cytometry, Humans, Immunophenotyping, Karyotype, Leukemia, Myeloid, Acute diagnosis, Male, Middle Aged, Mutation, Nucleophosmin, Young Adult, Leukemia, Myeloid, Acute genetics, Leukemia, Myeloid, Acute immunology, Nuclear Proteins genetics
- Abstract
This study was purposed to compare the immunophenotypic and clinical characteristics of NPM1 mutated acute myeloid leukemia with a normal karyotype under the similar constituent ratio of FAB subtypes. Immunophenotyping and NPM1 gene mutation type-A,B and D and other leukemic related fusion genes were detected by multiparameter flow cytometry and real time RT-PCR or PCR, respectively. 77 AML patients with a normal karyotype (NK) and mutated NPM1 gene (NPM1m(+)AML) detected by immunophenotyping assay were included in this study. 55 cases without NPM1 mutation (NPM1m(-)AML) and with normal karyotype were served as negative control. The results showed that there was significant difference between NPM1m(+)AML and NPM1m(-)AML in terms of sex, white blood count, platelet counts, blast, WT1 expression level, FLT3-ITD mutation positive rate and response to treatment. The characteristic immunophenotype is lower expression of early differentiation-associated antigens (CD34, HLA-DR, CD117, CD38), lymphocytic antigens (CD7, CD4, CD19, CD2) and higher expression of CD33 and CD123 (P < 0.05). When above features was further analyzed between the M1/2 and M4/5 subgroups in NPM1m(+)AML patients, the M1/2 cases retained a higher frequency in women and a higher WT1 expression level (P < 0.05) . Monocytic differentiation-associated antigens including HLA-DR and lymphocytic antigens CD7 were higher expressed and CD117 was lower expressed in M4/5 subgroup (P < 0.05). It is concluded that under condition of similar constituent ratio of M1/2 and M4/5 subtype and normal karyotype, NPM1m(+)AML patients have higher WT1 expression level and better response to treatment. The major immunophenotype features of NPM1m(+)AML patients are lower expression of early differentiation antigens and lymphoid lineage antigens and higher expression of CD33 and CD123. Monocytic differentiation-associated antigens only higher are expressed in M4/5 cases when compared with M1/2 cases within NPM1m(+) AML patients.
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- 2013
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80. [The immunophenotypic and clinical characteristics of NPM1 mutated acute myeloid leukemia patients].
- Author
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Liu YR, Chang Y, Ruan GR, Qin YZ, Lai YY, Shi HX, Wang YZ, Li LD, Jiang B, and Li JL
- Subjects
- Adolescent, Adult, Aged, Antigens, CD metabolism, Child, Child, Preschool, Female, HLA-DR Antigens immunology, Humans, Immunophenotyping, Leukemia, Myeloid, Acute diagnosis, Male, Middle Aged, Mutation, Nucleophosmin, Young Adult, Leukemia, Myeloid, Acute genetics, Leukemia, Myeloid, Acute immunology, Nuclear Proteins genetics
- Abstract
Objective: To compare the immunophenotypic and clinical characteristics between NPM1 mutated acute myeloid leukemia (AML) (NPM1m(+)AML) and unmutated AML(NPM1m(-)AML) not otherwise characterized (NOS) under similar FAB subtypes constituent ratio., Methods: Immunophenotyping and NPM1 gene mutation type-A, B and D and other leukemic related fusion genes were detected by multiparameter flow cytometry and real time RT-PCR or PCR, respectively. 104 AML patients with NPM1m(+)AML and performed immunophenotyping assay were included, 97 with NPM1m(-)AML., Results: There were significant difference between the two groups at presentation in terms of sex, white blood count(WBC), platelet counts (PLT), blast ratio, normal karyotype ratio, WT1 expression level, FLT3-ITD mutation positive rate and remission rate of first course of induction therapy (P < 0.05). On the immunophenotype, the expression of early differentiation antigens (CD34, HLA-DR, CD117, CD38), lymphocytic antigens (CD7, CD4, CD19, CD2), myeloid and monocytic differentiation-associated antigens (CD13, CD14, CD15) were lower, and that of CD33 as well as CD123 were higher in NPM1m(+)AML patients. Among them, only CD34, HLA-DR, CD7, and CD4 positive cases were significantly lower in NPM1m(+)AML group than in NPM1m(-)AML group (P < 0.05), the rest of them had significant difference in the number of positive cells (P < 0.05). Above features were further analyzed between the M1/M2 and M4/M5 subgroups. M1/M2 cases retained the women prominent and had a higher WT1 expression level (P < 0.05). The expression of monocytic differentiation-associated antigens including HLA-DR and lymphocytic antigens were higher and that of CD117 were lower in M4/M5 subtype (P < 0.05). Among them, the positive rates of HLA-DR, CD64, CD11b, CD10, CD15, and CD4 were significantly higher in M4/M5 than in M1/M2 in NPM1m(+)AML group (P < 0.05)., Conclusion: The most clinical characteristics in NPM1m(+)AML patients are consistent with reports, but some immunophenotype are different to the previous reports under similar FAB subtypes constituent ratio. The major immunophenotypic features of NPM1m(+)AML patients are lower expression of progenitor, myeloid and lymphoid lineage antigens. Monocytic differentiation-associated antigens are only higher expression in M4/M5 cases when comparison with M1/M2 cases within NPM1m(+)AML group.
- Published
- 2013
81. [A multicenter comparison study on the quantitative detection of bcr-abl (P210) transcript levels in China].
- Author
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Qin YZ, Cheng H, Cen JN, Geng SX, Li QH, Li XQ, Lin ZX, Ma DX, Qiao C, Wang YG, Li JL, Li LD, and Huang XJ
- Subjects
- Bone Marrow Cells, China, Fusion Proteins, bcr-abl genetics, Hospitals, Humans, Leukemia, Myelogenous, Chronic, BCR-ABL Positive genetics, Reverse Transcriptase Polymerase Chain Reaction, Fusion Proteins, bcr-abl isolation & purification, Leukemia, Myelogenous, Chronic, BCR-ABL Positive diagnosis
- Abstract
Objective: To investigate the comparability of bcr-abl (P210) transcript levels detected in different hospitals., Methods: Ten hospitals in China took part in the four times of sample exchange and comparisons from April, 2010 to August, 2011. The exchange samples were prepared by Peking University People's Hospital. Firstly, the BCR-ABL (P210)(+) cells from a newly diagnosed chronic myeloid leukemia patient were 10-fold serially diluted by BCR-ABL (P210)(-) cells and they covered 4 magnitudes. Then, TRIzol reagents were thoroughly mixed with cells in each tube. Every 12 samples (three samples per magnitude) were sent to the other 9 hospitals. The cell number of each sample was 8×10(6). The detection of bcr-abl transcript levels by real-time quantitative PCR were performed in every hospital according to their own protocols. Conversion factors (CF) were calculated using regression equation., Results: Differences in bcr-abl transcript levels did exist among results of 10 hospitals in each comparison. In general, the results of the most of hospitals were in line with the dilutions of cells. CF of every hospital fluctuated. Three hospitals had relatively stable CF, and their ranges were 2.8 - 5.2, 1.2 - 2.8 and 2.2 - 6.8, respectively; two hospitals had unstable CF with ranges 0.76 - 7.0 and 2.1 - 18.7; three hospitals couldn't be calculated CF one or two times because of the significant deviation of the results from the actually bcr-abl transcript levels, and their ranges of CF which could be calculated were 1.9 - 19.2, 3.6 - 7.6 and 0.18 - 14.7; One hospital only had two CF (3.3 and 5.0) because of the replacement of an important reagent during the period of comparisons., Conclusions: Comparability of bcr-abl (P210) transcript levels between different hospitals could be achieved through CF which acquired by sample exchange and comparison. The stable and reliable detection system is the premise to acquire correct CF.
- Published
- 2013
82. [Comparison of the basic characters of speech-evoked auditory brainstem response between school-age children and young adults].
- Author
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Liu JF, Wang NY, Fu X, Li L, Li JL, Wen XH, and Zhang HY
- Subjects
- Adult, Child, Female, Humans, Male, Young Adult, Evoked Potentials, Auditory, Brain Stem physiology, Speech
- Abstract
Objective: The aim of this study was to compare the basic characters of the speech-evoked auditory brainstem response (speech-ABR) between young adults and school-age children., Methods: Speech-ABR of thirty eight normal hearing subjects including eighteen school-age children and twenty young adults were recorded. The speech syllable/da/as stimulus sound was transmitted to right ear by insertion the earphones in speech-ABR test., Results: Response waves of speech-ABR in school-age children were similar to those in young adults, which contained the onset response (peak V and A), the transition (peak C), the frequency following responses (peak D, E and F) and the offset response (peak O). Both the latency and amplitude showed no significant difference in all waves between young adults and school-age children, except the latency of wave O and amplitude of wave F. The latency of O wave in school-age children (47.80 ± 0.38) ms were significantly shorter than that in adults (48.10 ± 0.40) ms (t = 2.330,P = 0.026). The amplitude of F wave in school-age children (-0.21 ± 0.15) µV were significantly larger than that in adults (-0.12 ± 0.08) µV (t = 2.146,P = 0.043)., Conclusion: Both the latency and amplitude of the speech ABR in school age children at 6 - 11 years old show the great similarity with the young adults, which indicate that the ability of speech processing of brainstem in children has completely reached maturity.
- Published
- 2012
83. [Clinical significance of CD34(+)CD38(+) and CD34(+)CD38(low/-) subgroups in bone marrow of patients with B lymphoblastic leukemia].
- Author
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Hao L, Liu YR, Wang YZ, Chang Y, Qin YZ, Li JL, Li LD, and Huang XJ
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- Adolescent, Adult, Bone Marrow immunology, Child, Child, Preschool, Female, Flow Cytometry, Humans, Immunophenotyping, Infant, Male, Middle Aged, Neoplasm, Residual immunology, Young Adult, ADP-ribosyl Cyclase 1 immunology, Antigens, CD34 immunology, Bone Marrow Cells immunology, Leukemia, B-Cell immunology
- Abstract
This study was purpose to investigate the biological characteristics of B lymphoblastic leukemia (B-ALL) between CD34 positive CD38 positive (CD34(+)CD38(+)) and CD34(+)CD38(low/-) subgroups and their clinical significance. Immunophenotyping of B cells in bone marrow of 54 patients with newly diagnosed CD34(+)B-ALL were analyzed by 4 color multiparametric flow cytometry (FCM). According to the different expression of CD38, the newly diagnosed patients with B-ALL were divided into two groups: CD34(+)CD38(+) subgroup and CD34(+)CD38(low/-) subgroup. BCR-ABL, TEL-AML1 fusion genes and WT1 gene were detected by real time RT-PCR simultaneously. After chemotherapy, minimal residual disease (MRD) was monitored by one tube of 7 color FCM. The average follow-up time was 12 months (range 1 - 28), the average follow-up interval was 2 months (range 1 - 5). The results showed that there was no significant differences such as WBC, Plt count and Hb level between the two groups at diagnosis, the positive rate of BCR-ABL, TEL-AML1 and WT1 gene was also no significantly different. After clinical complete remission (CR), MRD positive (MDR(+)) case rates were 28.57% (10/35) in CD34(+)CD38(+) subgroup and 68.42% (13/19) in CD34(+)CD38(low/-) subgroup (P < 0.01). The relapse rate between the two groups was 5.71% (2/35) in CD34(+)CD38(+) subgroup (relapse time at 94 and 245 d respectively) and 36.84% (7/19) in CD34(+)CD38(low/-) group [median relapse time was 263 d (range 46 - 468), P < 0.01]. The age distribution was analyzed in these two subgroups (> 16 or ≤ 16 years old), there was 8 (8/35) adult patients (> 16 years old) in CD34(+)CD38(+)group and 10 (10/19) adult patients in CD34(+)CD38(low/-) group (P < 0.05). It is concluded that CD34(+)CD38(low/-) phenotype is more often presented in adult patients and the CD34(+)CD38(low/-) patients with B-ALL are more likely to have MRD(+)and relapse after treatment.
- Published
- 2012
84. Quantitative chimerism kinetics in relapsed leukemia patients after allogeneic hematopoietic stem cell transplantation.
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Qin XY, Li GX, Qin YZ, Wang Y, Wang FR, Liu DH, Xu LP, Chen H, Han W, Wang JZ, Zhang XH, Li JL, Li LD, Liu KY, and Huang XJ
- Subjects
- Adolescent, Adult, Child, Female, Humans, Male, Middle Aged, Reverse Transcriptase Polymerase Chain Reaction, Young Adult, Hematopoietic Stem Cell Transplantation adverse effects, Leukemia genetics, Leukemia therapy, Transplantation Chimera genetics, Transplantation, Homologous adverse effects
- Abstract
Background: Chimerism analysis is an important tool for the surveillance of post-transplant engraftment. It offers the possibility of identifying impending graft rejection and recurrence of underlying malignant or non-malignant disease. Here we investigated the quantitative chimerism kinetics of 21 relapsed leukemia patients after allogeneic hematopoietic stem cell transplantation (HSCT)., Methods: A panel of 29 selected sequence polymorphism (SP) markers was screened by real-time polymerase chain reaction (RT-PCR) to obtain the informative marker for every leukemia patient. Quantitative chimerism analysis of bone marrow (BM) samples of 21 relapsed patients and 20 patients in stable remission was performed longitudinally. The chimerisms of BM and peripheral blood (PB) samples of 14 patients at relapse were compared., Results: Twenty-one patients experienced leukemia relapse at a median of 135 days (range, 30 - 720 days) after transplantation. High recipient chimerism in BM was found in all patients at relapse, and increased recipient chimerism in BM samples was observed in 90% (19/21) of patients before relapse. With 0.5% recipient DNA as the cut-off, median time between the detection of increased recipient chimerism and relapse was 45 days (range, 0 - 120 days), with 76% of patients showing increased recipient chimerism at least 1 month prior to relapse. Median percentage of recipient DNA in 20 stable remission patients was 0.28%, 0.04%, 0.05%, 0.05%, 0.08%, and 0.05% at 1, 2, 3, 6, 9, and 12 months, respectively, after transplantation. This was concordant with other specific fusion transcripts and fluorescent in situ hybridization examination. The recipient chimerisms in BM were significantly higher than those in PB at relapse (P = 0.001)., Conclusions: This SP-based RT-PCR assay is a reliable method for chimerism analysis. Chimerism kinetics in BM can be used as a marker of impending leukemia relapse, especially when no other specific marker is available. Based on our findings, we recommend examining not only PB samples but also BM samples in HSCT patients.
- Published
- 2012
85. [Application of auditory brainstem response (ABR) and 40 Hz auditory event related potential (40 Hz AERP) to the diagnosis of occupational noise-induced hearing impairment].
- Author
-
Xia YJ, Hao FT, Wang CY, Li JL, and Fu X
- Subjects
- Adult, Audiometry, Evoked Response, Audiometry, Pure-Tone, Auditory Threshold, Evoked Potentials, Auditory, Brain Stem, Hearing Loss, Noise-Induced physiopathology, Humans, Male, Middle Aged, Young Adult, Evoked Potentials, Auditory, Hearing Loss, Noise-Induced diagnosis, Noise, Occupational
- Abstract
Objective: To investigate the application of auditory brainstem response (ABR) and 40 Hz auditory event related potential (40 Hz AERP) to the diagnosis of occupational noise-induced hearing impairment and to provide the evidence for diagnosis of occupational deafness., Methods: Pure tone audiometry, ABR and 40 Hz AERP were performed in 54 workers occupationally exposed to noise. The thresholds of higher frequency band, 3 kHz and 4 kHz were compared with the threshold of ABR. The thresholds of auditory frequency ban and 0.5 kHz were compared with the threshold of 40 Hz AERP., Results: A better correlation was found between thresholds of ABR and higher frequency pure tone audiometry. There was a significant difference of thresholds between 40 kHz AERP and pure tone audiometry. The correction values of thresholds between 40 kHz AERP and pure tone audiometry in the light noise-induced hearing impairment group and the moderate noise-induced hearing impairment group were (16.43 ± 1.08) and (11.80 ± 1.12) dBn HL, respectively., Conclusion: In diagnosis of occupational noise-induced hearing impairment, the threshold of ABR can be used to estimate the hearing threshold of pure noise higher frequency. Because there is the significant difference of the thresholds between pure tone audiometry and 40 Hz AERP, the response threshold can not be served as the audiometry threshold, and the behavioral hearing thresholds can only be obtained by adjusting the response threshold with respective correction value.
- Published
- 2012
86. [Effects of Shenfu injection on hemodynamics and plasma E-selectin concentration in elderly patients undergoing total hip replacement surgery].
- Author
-
Yu WP, Hu J, Li JL, and Wen H
- Subjects
- Aged, Aged, 80 and over, Female, Humans, Male, Postoperative Period, Arthroplasty, Replacement, Hip, Drugs, Chinese Herbal pharmacology, E-Selectin blood, Hemodynamics drug effects
- Abstract
Objective: To investigate the effects of Shenfu (SF) injection on hemodynamics and plasma E-selectin concentrations in elderly patients undergoing total hip replacement (THR)., Methods: A total of 24 ASA II/III patients aged 65 to 85 years old were divided equally into two groups (n = 12). In Group S, SF injection was administered by peripheral intravenous infusion at initially 0.2 ml/kg and then 0.8 ml×kg(-1)×h(-1) until the end of operations. In Group N, normal saline was administered similarly. All patients received a post-operative regimen of patient-controlled epidural analgesia (PCEA). Blood samples were taken pre-operation (T(0)), immediately post-operation (T(3)) and 24 hours post-operation (T(4)) so as to detect the levels of E-selectin at these time points. mean arterial pressure (MAP), heart rate (HR) and central venous pressure (CVP) were continuously monitored and recorded at T(0), 15 min post-anesthesia (T(1)), 30 min post-anesthesia (T(2)) and T(3)., Results: The concentrations of E-selectin in Group N increased at T(3) and T(4) while those decreased in Group S [(119 ± 23) mg/L and (109 ± 23) mg/L vs (86 ± 15) mg/L, (83 ± 15) mg/L and (83 ± 12) mg/L vs (92 ± 37) mg/ L, all P < 0.01]. As compared with Group S, they were significantly higher in Group N (P < 0.05 or 0.01). There was no significant difference in CVP, HR and MAP between two groups., Conclusion: The activation of vascular endothelial cells is manifested by an elevated plasma concentration of E-selectin in the elderly patients undergoing THR. SF injection can inhibit the activation and exert no significant effects on the hemodynamics.
- Published
- 2011
87. [Evaluating the feasibility of nucleated cells isolated by lysis method for real-time quantitative RT-PCR].
- Author
-
Li LD, Qin YZ, Li JL, and Liu YR
- Subjects
- Adolescent, Adult, Child, Female, Humans, Male, Middle Aged, RNA, Messenger analysis, Young Adult, Leukemia genetics, RNA, Messenger genetics, Reverse Transcriptase Polymerase Chain Reaction methods
- Abstract
This study was aimed to investigate whether difference exists between real time RT-PCR results of nucleated cells isolated by lysis method and mononuclear cells isolated by gradient concentration method. 14 bone marrow samples from leukemia patients (7 samples of AML-M(2), 1 of AML-M(4), 1 of AML-M(4)EO, 1 of AML-M(6), 1 of APL and 3 of CML) were collected. Each sample was divided into 2 parts, and was used to isolate mononuclear cells by Ficoll-Hypaque gradient centrifugation, and other was used to isolate nuclear cells by lysis method. The RNA extraction and detection of internal reference gene ABL for all of samples were performed by RT-PCR, and mRNA expression levels in 3 samples of BCR/ABL, 6 of AML/ETO, 1 of CBFβ-MHY11, 1 of WT1 and 6 PRAME were detected by RT-PCR. The results showed that ABL copies of all samples were over 3 × 10(4), and there was significant difference in ABL copies between each pair of samples by lysis and gradient centrifugation method (p > 0.05). There was also no significant difference in every detected mRNA levels between 14 pair samples (p > 0.05). It is concluded that the lysis method may be useful one for extracting RNA from nuclear cells, and can adopt as a routing detection method for simultaneously extracting RNA from many samples to detect leukemia specific mRNA by using RT-PCR.
- Published
- 2011
88. [Synergistic killing effect of the conditionally replicating adenoviruses carrying programmed cell death 5 gene and etoposide on K562 cells].
- Author
-
Xie M, Chang Y, Niu JH, Zhang Y, Li JL, Wu HP, Li LF, Huang XJ, and Ruan GR
- Subjects
- Adenoviridae genetics, Cell Survival, Genetic Vectors, Humans, K562 Cells, Antineoplastic Agents, Phytogenic pharmacology, Apoptosis, Apoptosis Regulatory Proteins genetics, Etoposide pharmacology, Neoplasm Proteins genetics
- Abstract
The expression levels of programmed cell death 5 (PDCD5) are down-regulated in many malignancies. SG611-pdcd5, a recombinant conditionally replicative adenovirus carrying pdcd5 gene expression cassette, can evidently kill the leukemic cells and protect selectively the normal cells. The purpose of this study was to investigate the synergistic killing effect of SG611-pdcd5 and low-dose etoposide (VP-16) on K562 cells. K562 cells were treated with different concentrations of VP-16 or different multiplicities of infection (MOI) of SG611-pdcd5. After 48 hours of incubation the cell viability was determined by using MTT assay. The results showed that the cell viability of SG611-pdcd5 (MOI = 40) plus VP-16 (0.5 µg/ml) group significantly decreased as compared with single SG611-pdcd5 (MOI = 40) treatment group or single VP-16(0.5 µg/ml) treatment group (42.00 ± 5.75% vs 59.45 ± 4.12%; 42.00 ± 5.75% vs 82.91 ± 3.41%, respectively, both p < 0.05). The synergistic killing effect of SG611-pdcd5 plus VP-16 was higher than that of PDCD5 protein plus VP-16 or that of non-replicating adenovirus carrying pdcd5 (Ad-pdcd5) plus VP-16 (both p < 0.05). The cell viability of VP-16 (4.0 µg/ml) plus SG611-pdcd5 (MOI = 40) group, VP-16 (4.0 µg/ml) plus proPDCD5 (40 µg/ml) group and VP-16 (4.0 µg/ml) plus Ad-pdcd5 (MOI = 80) group was 37.09 ± 1.89%, 52.36 ± 1.64% and 73.64 ± 4.33%, respectively. It is concluded that SG611-pdcd5 can promote K562 cell death induced by low-dose VP-16. The combination of SG611-pdcd5 and VP-16 can enhance the killing effect on leukemic cells.
- Published
- 2010
89. [Effects of Shenfu injection on coagulation function, D-dimer and plasma p-selectin in elderly patients during total hip replacement surgery].
- Author
-
Hu J, Li JL, Wen H, and Yu WP
- Subjects
- Aged, Aged, 80 and over, Blood Coagulation, Blood Coagulation Tests, Female, Humans, Male, Postoperative Period, Arthroplasty, Replacement, Hip, Drugs, Chinese Herbal therapeutic use, Fibrin Fibrinogen Degradation Products metabolism, P-Selectin blood
- Abstract
Objective: To investigate the effects of SF injection on coagulation function, p-selectin and D-dimer concentrations in plasma of elderly patients undergoing total hip replacement (THR)., Methods: Twenty-four ASA II/III patients aged 65 to 85 were divided equally into two groups (n = 12). In Group S, SF injection was administered by peripheral intravenous infusion at initially 0.2 ml/kg and then 0.8 ml (kg/h) until the end of operations. In Group N, normal saline was administered similarly. All the patients were given patient-controlled epidural analgesia (PCEA) after operations. Blood samples were taken before operations (T(0)), immediately post-operation (T(1)) and 24 h post-operation to detect the levels of p-selectin, D-dimer, blood platelet count and clotting time at all time points., Results: The concentrations of p-selectin and D-dimer in Group N increased at T(1) and T(2) while those in Group S decreased at T(1) (P < 0.05). Compared with Group N, they were significantly lower in Group S (P < 0.05). There was no significant difference at T(2) between two groups., Conclusion: The plasma concentrations of p-selectin and D-dimer increase obviously in elderly THR patients. SF injection can decrease the plasma concentrations of p-selectin and D-dimer in patients during surgery. Thus it may protect the vascular endothelial cells and attenuate the activation of platelet and fibrinolysis.
- Published
- 2010
90. [Abnormal expression of programmed cell death 5 gene in multiple myeloma patients].
- Author
-
Bao L, Ruan GR, Lu XJ, Zhang XH, Lu J, Niu JH, Zhang Y, Xie M, Qin YZ, Li LD, Li JL, Liu YR, Chen SS, and Huang XJ
- Subjects
- Adult, Aged, Bone Marrow Cells pathology, Case-Control Studies, Female, Gene Expression, Humans, Male, Middle Aged, Young Adult, Apoptosis Regulatory Proteins genetics, Multiple Myeloma genetics, Multiple Myeloma pathology, Neoplasm Proteins genetics
- Abstract
The aim of this study was to investigate the gene expression of programmed cell death 5 (pdcd5) in plasma and bone marrow cells from patients with multiple myeloma (MM). Enzyme liked immunosorbent assay (ELISA) and real-time quantitative reverse transcription polymerase chain reaction (RQ-RT-PCR) were used to examine pdcd5 gene expression in plasma and marrow cells in 45 MM patients and 20 normal controls. The results showed that serum levels of PDCD5 protein in 45 MM patients were lower significantly compared with the normal controls and 20 responsive patients after chemotherapy, their plasma levels were (16.91 +/- 0.28) ng/ml, (19.11 +/- 0.29) ng/ml and (17.94 +/- 0.154) ng/ml respectively (p < 0.05). The pdcd5 gene expression levels detected by RQ-RT-PCR in 45 MM patients were lower significantly compared with the normal controls, their pdcd5 gene expression levels were 0.64 +/- 0.47 and 1.28 +/- 1.21 respectively (p < 0.05). It is concluded that the PDCD5 protein expression levels are low in patients with MM. These findings suggest that abnormal expression of pdcd5 may be involved in the pathogenesis of MM.
- Published
- 2010
91. [Abnormally lower expression of cmtm5 gene in bone marrow cells from patients with multiple myeloma].
- Author
-
Niu JH, Bao L, Zhang Y, Li JL, Li LD, Xie M, Qin YZ, Lai YY, Jiang Q, Shi HL, Liu YR, Jiang B, Chen SS, Huang XJ, and Ruan GR
- Subjects
- Adult, Aged, Aged, 80 and over, Bone Marrow Cells pathology, Case-Control Studies, Chemokines genetics, Female, Humans, MARVEL Domain-Containing Proteins, Male, Middle Aged, Multiple Myeloma pathology, Neoplasm Staging, Tumor Suppressor Proteins genetics, Young Adult, Bone Marrow Cells metabolism, Chemokines metabolism, Multiple Myeloma metabolism, Tumor Suppressor Proteins metabolism
- Abstract
This study was aimed to detect the expression level of cmtm 5 (CKLF-like MARVEL transmembrane domain containing member 5) gene in the bone marrow cells from patients with multiple myeloma (MM), and to investigate the correlation between the expression level of cmtm5 and various clinical characteristics. Real-time quantitative reverse transcription polymerase chain reaction (RQ-RT-PCR) was used to measure the expression levels of cmtm5 gene in the bone marrow cells collected from MM patients, and the MM cell lines, namely, RPMI8226 and CZ1 cells. The normal donor marrow specimens were used as the reference. The ratio of cmtm5 copy number to abl (Abelson murine leukemia viral oncogene homolog) gene copy number was used for indicating the expression level. The results showed that the expression level of cmtm5 gene was significantly down-regulated in bone marrow cells of 51 untreated or relapsed/refractory MM patient as compared to those of normal donor marrow cells (0.047+/-0.062 for the untreated or relapsed/refractory MM patients versus 0.255+/-0.333 for the normal, p<0.01). According to the International Staging System (ISS), the cmtm5 expression level in marrow cells of patients in ISS III stage was significantly lower than that in patients in ISS I stage (0.034+/-0.034 for the ISS III stage versus 0.103+/-0.109 for ISSI stage, p<0.01). Similarly, lower expression levels of cmtm5 gene were also found in two human MM cell lines (0.014+/-0.009 for RPMI8226 cells and 0.004+/-0.006 for CZ1 cells). After the MM patients were effectively treated, their expression levels of cmtm5 gene significantly increased (0.020+/-0.005 for the untreated patients versus 0.227+/-0.038 for the effectively treated patients, p<0.01). A significant negative correlation was observed between the expression level of cmtm5 gene and the number of bone marrow plasma cells (r=-0.307, p<0.05). However, the correlation was not found between the expression level of cmtm5 gene and the clinical characteristics, such as gender, age, hemoglobin level, or M-protein level, etc. It is concluded that the expression level of cmtm5 gene is abnormally lower in the bone marrow cells from the MM patients, and are associated with ISS stages. Furthermore, the expression level of cmtm5 gene is negatively correlated with the number of bone marrow abnormal plasma cells in MM patients, which suggests that the abnormally lower expression of cmtm5 may be involved in the pathogenesis of the MM patients.
- Published
- 2010
92. [Method of measuring sound localization for 4-year-old children on the horizontal plane.].
- Author
-
Zhang J, Wang X, Wang H, Liu JF, Song PL, Wen XH, Li JL, and Wang NY
- Subjects
- Child, Humans, Sound Localization
- Abstract
Objective: To investigate the effective way to test 4-year-old children's ability of sound localization in the horizontal plane., Methods: Using minimum audible angle (MAA) measure procedure on the basis of conditioned play audiometry, sound localization test was conducted for 4-year-old children at 0 degrees , +/- 45 degrees , +/- 90 degrees , +/- 135 degrees and 180 degrees standard positions in the horizontal plane., Results: The outcome of sound localization test for 4-year-old children separately were: MAA (0 degrees ) = (3.80 +/- 0.71) degrees , MAA (-45 degrees ) = (7.70 +/- 1.27) degrees , MAA (45 degrees ) = (7.10 +/- 1.39) degrees , MAA (-90 degrees ) = (8.15 +/- 2.38) degrees , MAA (90 degrees ) = (7.61 +/- 2.47) degrees , MAA (-135 degrees ) = (8.85 +/- 2.70) degrees , MAA (135 degrees ) = (8.30 +/- 1.42) degrees , MAA (180 degrees ) = (5.20 +/- 1.27) degrees . The MAA of eight standard positions were less than 10 degrees , and the MAA (0 degrees ) was the smallest one., Conclusions: Our findings suggest that MAA test procedure on the basis of conditioned play audiometry could be used to evaluate the ability of sound localization in 4-year-old children.
- Published
- 2009
93. [Quantitative analysis of dendritic cell subsets in bone marrow of patients with acute myeloid leukemia].
- Author
-
Lin HR, Wang YZ, Wang DX, Chang Y, Hao L, Qin YZ, Li JL, Li LD, Huang XJ, and Liu YR
- Subjects
- Adolescent, Adult, Aged, Case-Control Studies, Child, Dendritic Cells immunology, Female, Flow Cytometry, Humans, Immunophenotyping, Male, Middle Aged, Young Adult, Bone Marrow Cells cytology, Dendritic Cells cytology, Leukemia, Myeloid, Acute immunology
- Abstract
In order to study the quantity of dendritic cell (DC) subsets of bone marrow in patients with acute myeloid leukemia (AML), the bone marrow aspirate were collected from 77 newly diagnosed AML patients and from 30 healthy persons. The quantity of DC subsets (myeloid dendritic cells, mDC and plasmacytoid dendritic cells, pDC) were detected by flow cytometry and analysed by 3-color and 4-color cytometric gate. Based on the conventional 3-color panel, mDC were identified by Lin-HLA-DR+CD11c+ and pDC were identified by Lin-HLA-DR+CD123+. Based on the 4-color panel, mDC were identified by Lin-HLA-DR+CD11c+ BDCA-1+ and pDC were identified by Lin-HLA-DR+CD123+BDCA-2+. The results showed that a reduction of mDC was found in 74.0% (57/77) and 58.4% (45/77) patients, a reduction of pDC was found in 90.9% (70/77) and 46.8% (36/77) patients respectively by 3-color and 4-color cytometric analysis. Meanwhile an expansion of mDC was showed in 19.5% (15/77) and 22.1% (17/77) patients, an expansion of pDC was showed in 1.3% (1/77) and 27.3% (21/77) patients respectively by 3-color and 4-color cytometric analysis. In subtypes of AML-M2, AML-M3 or AML-M4/5, 81.4%, 100% and 42.1% patients showed mDC decrease and 88.4%, 100% and 89.5% patients showed pDC decrease respectively by 4-color cytometric analysis. It is concluded that the 4-color cytometric gate is better method for detection of mDC and pDC from bone marrow of newly diagnosed AML patients as compared with 3-color cytometric gate, the majority of AML patients showed reduction of mDC and pDC. The percentages of patients with mDC normal or mDC increase in AML-M4/5 subtypes are more than that in AML-M2/3 subtypes, while the pDC does not show difference between AML subtypes.
- Published
- 2009
94. [Construction and verification of a novel triple-regulated oncolytic adenovirus carrying gene Pdcd5].
- Author
-
Xie M, Wu HP, Li LF, Niu JH, Chang Y, Li JL, Huang XJ, and Ruan GR
- Subjects
- Genetic Therapy methods, Promoter Regions, Genetic, Telomerase genetics, Adenoviridae genetics, Apoptosis Regulatory Proteins genetics, Neoplasm Proteins genetics, Oncolytic Viruses genetics
- Abstract
The purpose of this study was to construct a recombinant conditionally replicating adenovirus (CRAd) expressing programmed cell death 5 (pdcd5). Pdcd5 gene was inserted in the E3 region of SG600-a CRAd in which the key genes for virus replication E1a and E1b were controlled under the human telomerase reverse transcriptase promoter (hTERTp) and the hypoxia response element (HRE) respectively, and with a deletion of 24 nucleotides within CR2 region of E1a. The insertion and orientation of all recombined plasmids were confirmed by restriction enzyme digestion and polymerase chain reaction (PCR). The infection efficiencies of a recombined virus carrying enhanced green fluorescent protein (EGFP) in leukemic cell lines were observed by using fluorescence microscope. The relative pdcd5 expression levels of K562 after being infected with SG611-pdcd5 were detected by real-time quantitative PCR. The results showed that the construction of SG611-pdcd5 was completed and confirmed. Pdcd5, hTERTp, HRE, skeleton and fiber11 of recombinant adenovirus SG611-pdcd5 were successfully amplified. The infection efficiencies of SG611-EGFP were all above 70% in both leukemic K562 and MEG-01 cell lines. SG611-pdcd5 expressed pdcd5 with high efficiency in leukemic cells as compared with Ad-pdcd5 or SG611 (p < 0.001). The expression level of pdcd5 increased gradually along with the increase of MOI. It is concluded that the triple-regulated adenovirus of SG611-pdcd5 containing the pro-apopro-tic gene pdcd5 has been successfully established with high pdcd5 expression level in leukemic cells, indicating that the recombinant adenovirus, SG611-pdcd5, promises further development of targeted tumor gene therapy.
- Published
- 2009
95. [Expression of 5 genes in CD7 positive acute myeloid leukemia stem/progenitor cells from bone marrow].
- Author
-
Wu HH, Cao H, Wang YZ, Wang DX, Lin HR, Qin YZ, Chang Y, Hao L, Li LD, Li JL, Ruan GR, Huang XJ, and Liu YR
- Subjects
- Adolescent, Adult, Aged, Antigens, CD7 immunology, Bone Marrow Cells immunology, Case-Control Studies, Female, Flow Cytometry, Gene Expression Regulation, Leukemic, Hematopoietic Stem Cells chemistry, Hematopoietic Stem Cells immunology, Humans, Leukemia, Myeloid, Acute immunology, Male, Middle Aged, Stem Cells immunology, Young Adult, Bone Marrow Cells chemistry, Leukemia, Myeloid, Acute genetics, Stem Cells chemistry
- Abstract
This study was aimed to investigate abca5, mdr-1, kdr, dapk and irf-1 expressions in leukemia stem/progenitor cells (LSC) from CD7 positive acute myeloid leukemia, the expression of these 5 genes in mononuclear cells (MNC) from 15 normal bone marrow (NBM) and 16 AML patients bone marrow (AML BM) specimen were detected by real-time quantitative PCR (RQ-PCR). CD34(+)CD38(+) progenitor cells and CD34(+)CD38(-)Lin(-) stem cells were sorted by flow cytometry (FCM) from the MNCs of 10 NBM and 21 AML BM specimen. These 5 gene expressions in the sorted cells were detected by small amount cell RQ-PCR. The results showed that these 5 genes above mentioned all expressed in NBM-MNC, in which the expression levels of irf-1 and dapk were highest with the relative expression levels 4.08 and 3.86, the expression levels of abca 5 and mdr-1 were in the middle with the relative expression 0.49 and 0.84 respectively, the kdr expression was lowest with the relative expression level 0.02. In CD34(+)CD38(+) progenitor cells, the expression level of kdr increased dramatically (p < 0.05) while irf-1 and dapk dramatically decreased (p < 0.05). There was no obvious change of expression in the rest 2 genes. In CD34(+)CD38(-) stem cells the expression level of these 5 genes all increased nearly 2 times as much as that in CD34(+)CD38(+) progenitor cells, but kdr increased 3 times as much, and the increase of kdr and irf-1 expressions was of statistical significance (p < 0.05). Compared with the NBM, expression levels of 5 genes in AML-MNC decreased, and out of them abca 5, mdr-1, kdr and dapk were decreased most remarkably (p < 0.05). Comparison between AML CD34(+)CD38(+) cells and AML MNC showed that the expression level of irf-1 and dapk were decreased dramatically (p < 0.05) while the rest 3 genes increased their expression with statistical significance (p < 0.05). The expression levels of these 5 genes were higher in CD34(+)CD38(-) cells than those in CD34(+)CD38(+) stem cells, and the increase of kdr and irf-1 expressions showed statistical difference (p < 0.05). These 5 genes expression levels were all higher than those in CD34(+)CD38(+) cells whether in AML CD34(+)CD38(-)CD7(+) cells or CD34(+)CD38(-)CD7(-) cells. The increase of kdr expression in CD34(+)CD38(-)CD7(+) cells as well as kdr and irf-1 expressions in CD34(+)CD38(-)CD7(-) cells were all of statistical significance (p < 0.05). In conclusion the expression level of kdr in NBM was highest in stem cells while dapk and irf-1 were highest in differentiated cells. The expression levels of these 5 genes in CD34(+)CD38(-)Lin(-) stem cells were higher than those in CD34(+)CD38(+) progenitor cells. The gene expressions in AML CD34(+)CD38(-)CD7(+) cells and CD34(+)CD38(-)CD7(-) cells are in accordance with the characteristics of stem cells.
- Published
- 2009
96. [Relationship of immunophenotypic features with minimal residual disease detection and gene types in 221 cases of acute promyelocytic leukemia].
- Author
-
Wang YZ, Qin YZ, Jiang B, Zhu HH, Chang Y, Hao L, Li JL, Li LD, Chen SS, Huang XJ, and Liu YR
- Subjects
- Adolescent, Adult, Aged, Antigens, CD genetics, Child, Female, Flow Cytometry, Humans, Immunophenotyping, Leukemia, Promyelocytic, Acute diagnosis, Male, Middle Aged, Neoplasm, Residual diagnosis, Young Adult, Leukemia, Promyelocytic, Acute genetics, Leukemia, Promyelocytic, Acute immunology, Neoplasm, Residual genetics
- Abstract
This study was aimed to investigate the relationship of immunophenotypic features with minimal residual disease (MRD) detection and gene types in APL patients. Immunophenotypes were analyzed in 221 newly diagnosed APL patients by using four-color flow cytometry. Among of them, CD123 antibody was examined in 87 patients and the fused gene pml-raralpha were detected by PCR in 196 specimens simultaneously. The results of immunophenotyping demonstrated that the positive percentages of CD123, CD33 and CD9 in newly diagnosed APL patients were 100%, 99.1% and 96.0% respectively, and mean percentages of positive cells in positive patients were all around 90%. Although the positive rates of CD117, CD13, CD38 and CD64 were all above 96%, but the mean percentages of positive cells in different positive patients were diverse and average percentages of positive cells were about 70%. CD15, CD56 and CD11b were expressed in some patients, but CD34 and HLA-DR were rarely expressed in the majority of patients, and average positive percentages were all lower. Among 196 newly diagnosed APL patients, bcr1, bcr2 and bcr3 expressions were 63.3%, 4.6% and 32.1% respectively. The results showed a strong correlation of positive expression of CD34 with bcr3 isoform. When cut-off value was chosen as 20%, the proportions of CD34 positive patients in bcr3 and bcr1 cases were 15.4% (10/65) and 3.3% (4/121) separately, which had a significant difference (p < 0.05). When cut-off value was 10%, bcr3 cases had a significantly higher percentage of CD34 positive, compared with bcr1 cases (p < 0.001), which was 47.7% (31/65) and 5.8% (7/121) respectively. However, there was no statistically significant difference on the other antigens between the two groups. Bcr3 isoform was highly indicated when CD34 was positive and non- large side scatter (NL-SSC) was shown in APL cells. It is concluded that there is a unique characteristics of immunophenotyping, and antigens such as CD123, CD33 and CD9 are more applicable to the detection of MRD in APL patients. The positive expression of CD34 and NL-SSC are associated with bcr3 isoform, and the relationship between gene type and antigen expression can be suggested more accurately when the cut-off value is chosen as 10%.
- Published
- 2009
97. [Elicitation of mismatch negativity and its influence from stimuli deviant in the healthy youth].
- Author
-
Chen Z, Wang NY, Li JL, Xin ZH, and Wen XH
- Subjects
- Adult, Audiometry, Pure-Tone, Female, Humans, Male, Reaction Time, Reference Values, Young Adult, Acoustic Stimulation, Evoked Potentials, Auditory physiology
- Abstract
Objective: To work out the elicitation plan, obtain the mismatch negativity (MMN) and get out the laboratory normal value as well as to study the influence to MMN from the deviation of auditory stimuli., Methods: Hearing test of the tone burst stimulation was performed on 21 healthy young volunteers according to oddball stimulation sequence. Each subject was performed two kinds of auditory stimuli including frequency deviant stimuli and intensity deviant stimuli, and of each one included three series of stimulation. MMN was gained by subtracting the ERP of deviant stimuli from the ERP of standard stimuli. The latency and amplitude of each MMN were recorded, and then the effect of the deviant extent for MMN was analyzed., Results: By this setup the MMN of normal young people was recorded and normal value of latency and amplitude of MMN were got. In the group of frequency deviant stimuli, the MMN latency [(155.81 +/- 29.08) ms], if the frequency was up to 2000 Hz, was shorter than that when the frequency deviance was 1000 Hz [(182.89 +/- 45.85) ms, (183.32 +/- 43.33) ms] (P = 0.033, 0.030); when the deviant extent were the same, the latency had no obvious difference if changing the frequency of the standard and deviant stimuli (P = 0.973); the MMN amplitude of three groups [(3.85 +/- 2.22) microV, (2.90 +/- 2.05) microV, (2.66 +/- 2.12) microV] had no obvious difference among them (P > 0.05). In the group of intensity deviant stimuli, the MMN latency [(157.04 +/- 34.87) ms], if the frequency was up to 20 dB, was shorter (P = 0.025, 0.017) than that when the intensity deviance was 10 dB [(184.46 +/- 38.05) ms, (186.24 +/- 42.36) ms]. When the deviant extent were the same, the latency had no obvious difference (P = 0.882) if changing the intensity of the standard and deviant stimuli but only group 4 and group 6 [(3.41 +/- 1.64) microV, (2.37 +/- 1.47) microV] were different in evidence (P = 0.031) while the others had no obvious difference (P = 0.524, 0.122)., Conclusions: MMN was only related to the difference between standard stimuli and deviant stimuli, but there was no relationship between MMN and the notice, which indicate that MMN could objectively reflect the capability of brain to detect the change of stimuli. MMN is the representation of brain high-level sensory function.
- Published
- 2009
98. [PRAME mRNA expression in newly diagnosed acute myeloid leukemia patients and its application to monitoring minimal residual disease].
- Author
-
Qin YZ, Li JL, Zhu HH, Li LD, Chang Y, Hao L, Wang YZ, Jiang B, Lu XJ, Liu YR, Huang XJ, and Chen SS
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Antigens, Neoplasm genetics, Child, Child, Preschool, Female, Follow-Up Studies, Humans, Leukemia, Myeloid, Acute metabolism, Male, Middle Aged, Neoplasm, Residual metabolism, RNA, Messenger genetics, Reverse Transcriptase Polymerase Chain Reaction, Young Adult, Antigens, Neoplasm metabolism, Leukemia, Myeloid, Acute diagnosis, Neoplasm, Residual diagnosis
- Abstract
Objective: To investigate the expression level of preferentially expressed antigen of melanoma (PRAME) mRNA in newly diagnosed acute myeloid leukemia (AML) patients and evaluate its usefulness for detecting minimal residual disease (MRD)., Methods: PRAME mRNA levels were detected in bone marrow samples from 142 newly diagnosed AML patients (72 of them didn't express any specific fusion gene) by TaqMan based real-time quantitative PCR methods, and were serially monitored in 60 bone marrow samples from 9 follow-up patients (2 of them without specific fusion gene), including 3 in continuous complete remission, 6 in hematological relapse. Bone marrow samples from 22 bone marrow donors (NBM) were served as normal controls. Samples from 7 AML1-ETO (+) M2 patients were detected for AML1-ETO mRNA simultaneously. abl was selected as control gene, PRAME and AML1-ETO mRNA levels were expressed by their copies/abl copies in percentage., Results: All NBM samples expressed PRAME mRNA and the upper limit was 0.28%. For all newly diagnosed AML patients, median PRAME mRNA level was 3.97% (0.00%-714.97%), 76.8% of them was higher than 0.28%, 54.9% had over 1-log increasing and 26.1% had over 2-log increasing. For patients without specific fusion gene, median PRAME mRNA level was 0.60% (0.00%-408.72%), 56.3% of them was over 0.28%, 32.4% and 11.3% had over 1-log and 2-log increasing, respectively. There was a significant difference in PRAME mRNA levels between subtypes of AML patients (P<0.01). AML1-ETO (+) M2 patients expressed the highest levels (all P<0.01), followed by acute promyelocytic leukemia patients with S type PML-RAR alpha fusion gene. PRAME and AML1-ETO mRNA levels of follow up patients displayed similar kinetic patterns, and correlated well in 43 follow up samples (r=0.88, P<0.01). PRAME mRNA levels in 3 hematological relapsed patients increased above 0.28% 1-4 months ahead relapse, and in other 3 relapsed patients the levels never decreased to normal range even in remission., Conclusions: PRAME mRNA could be used to monitor MRD for AML patients with higher than normal levels, and it increases over or persistently higher than normal range predicts hematological relapse.
- Published
- 2008
99. [Adenovirus-mediated PDCD5 gene transfer sensitizes apoptosis of K562 cells induced by etoposide].
- Author
-
Ruan GR, Chen SS, Chang Y, Li JL, Qin YZ, Li LD, Hao L, Fu JY, Liu YR, and Huang XJ
- Subjects
- Adenoviridae genetics, Antineoplastic Agents, Phytogenic pharmacology, Apoptosis Regulatory Proteins metabolism, Humans, K562 Cells, Neoplasm Proteins metabolism, RNA, Messenger metabolism, Recombinant Proteins genetics, Recombinant Proteins metabolism, Adenoviridae metabolism, Apoptosis drug effects, Apoptosis Regulatory Proteins genetics, Etoposide pharmacology, Neoplasm Proteins genetics, Transfection
- Abstract
This study was purposed to investigate the effect of adenovirus-mediated transfer of PDCD5 gene on apoptosis of K562 cells induced by etoposide. Recombinant adenovirus PDCD5 (Ad-PDCD5), control vectors Ad-null and Ad-eGFP were constructed by AdMax vector system respectively. After K562 cells were transfected by Ad-PDCD5, Ad-null or Ad-eGFP with different multiplicity of infection (MOI), the expression level of the PDCD5 gene was examined by RQ-RT-PCR assay. The effects of etoposide in combination with Ad-PDCD5 on the proliferation and apoptosis of K562 cells were measured by using MTT assay and flow cytometry with Annexin-V-FITC/PI dual labeling technique, respectively. The results showed that the transfection efficiencies of Ad-eGFP in K562, Jurkat and CEM cells ranged from 60% to 86%. Expression level of PDCD5 gene in K562 cells was evidently increased following transfection with Ad-PDCD5. The Ad-PDCD5 synergistically enhanced the apoptotic percentage of K562 cells induced by VP-16, as compared with that of Ad-null + VP16 and VP-16 alone respectively. It is concluded that Ad-PDCD5 may be a potential agent for enhancing the chemotherapy effect.
- Published
- 2007
100. [Detection of JAK2V617F mutation in patients with myeloproliferative disorders with TaqMan-MGB probe].
- Author
-
Ruan GR, Chen SS, Li LD, Liu YR, Qin YZ, Li JL, Ma X, Wang FR, Jiang Q, Jiang B, Liu KY, and Huang XJ
- Subjects
- Adult, Amino Acid Substitution, Base Sequence, DNA Mutational Analysis methods, DNA Probes genetics, Humans, Molecular Sequence Data, Polymerase Chain Reaction methods, Reproducibility of Results, Janus Kinase 2 genetics, Mutation, Myeloproliferative Disorders genetics
- Abstract
Objective: To develop a novel platform for detection of the JAK2V617F mutation in patients with myeloproliferative disorders (MPD) by real-time quantitative PCR., Methods: TaqMan-MGB probe was constructed. Peripheral blood samples were collected from 374 MPD patients, 76 with polycythemia vera (PV), 38 with chronic myelogenous leukemia (CML), and 115 with essential thrombocythemia (ET), and 19 with idiopathic myelofibrosis (IMF). Peripheral blood samples from 65 patients with acute myelogenous leukemia (AML), 30 patients with acute lymphoblastic leukemia, 8 patients with chronic lymphoblastic leukemia, and 7 patients with non-Hodgkin's lymphoma and 16 cases of normal donor bone marrow were used as controls. Genomic DNA or RNA was extracted and reversely transcrtibed into cDNA. TaqMan-MGB probe was used to detect the JAK2V617F mutant in MPD. Furthermore, 168 specimens underwent allele specific PCR and 8 specimens underwent sequencing. This method was used on both DNA and cDNA specimens from 38 MPD patients simultaneously so as to test the consistency., Results: The JAK2V617F mutation rates of the PV, ET, and IMF patients were 53 (70%), 59 (51%), (58%) respectively. JAK2V617F mutation was found in only one of the 65 AML patients and was not identified in other control specimens. Both the results of allele specific PCR and of sequencing were consistent with the result of TaqMan-MGB probe method., Conclusion: JAK2V617F mutation is widespread in Chinese MPD patients. Real-time quantitative PCR with TaqMan MGB probe can be used for rapid and accurate detection of the JAK2V617F mutation.
- Published
- 2007
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