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This study introduces an innovative approach, Ultrasonic Sonication Pretreatment Sparger-based Microwave Assisted Extraction (U Sp-MAE), designed to enhance lemongrass essential oil extraction. Addressing the challenge of maximizing yield and quality, our work systematically optimized key extraction parameters. Results demonstrated a significant improvement over conventional methods, with the highest yield of 1.89% achieved under specific conditions: a Sparger Flow rate of 4 L/min, Ultrasound Power of 700 W, Sonication Time of 5 min, and Liquid-to-Solid Ratio of 5 mL/g. This method not only advances essential oil extraction but holds promise for broader applications in society, showcasing its potential impact on sustainable and efficient resource utilization. [ABSTRACT FROM AUTHOR]

Objective: Although cancer therapy suppresses recurrence and prolongs life, it may be accompanied by strong side effects; thus, there is a strong demand for the development effective treatments with fewer side effects. Cancer therapy using plant-derived essential oils is attracting attention as one promising method. This study investigated the antitumor effects of essential oil volatiles on breast cancer cells and identifies four essential oils that display antitumor activity. Methods: Breast cancer cells were cultured in a 96-well plate, then one of twenty essential oils was added dropwise to the central well. The plate was incubated at 37 °C for 48 h and the effect of the volatile components of each essential oil on the surrounding breast cancer cell growth ability was examined using an MTT assay. Gas chromatography was used to investigate the concentration of the transpiration components that may affect cancer cells. Results: Of the 20 essential oils, Lemongrass, Lemon myrtle, Litsea, and Melissa displayed strong anti-tumor effects. These essential oils inhibited the growth of nearby breast cancer cells, even when diluted more than 500-fold. The transpiration component of lemon Myrtle showed the strongest antitumor effect, but was the least cytotoxic to mononuclear cells in normal peripheral blood (PBMC). Each of these essential oils contained a very large amount of citral. The IC50 against breast cancer cells when citral was volatilized from each essential oil was 1.67 µL/mL for geranial and 1.31 µL/mL for neral. Volatilized citral alone showed strong anti-proliferation and infiltration-inhibiting effects. Conclusion: The transpiration components of Lemongrass, Lemon myrtle, Litsea, and Melissa are thought to inhibit breast cancer cell proliferation due to their high levels of citral. [ABSTRACT FROM AUTHOR]

Lemongrass (genus Cymbopogon) is commonly used in foods, beverages, cosmetics, pharmaceuticals, and material science. Cymbopogon ambiguus A. Camus, the Australian Native Lemongrass, is a lesser-known member of the genus Cymbopogon, and research on this plant is scarce. Australian Indigenous people use the stalks and leaves of C. ambiguus as teas. Dried chopped leaves are also used as herbs in cooking. The aim of this study was to determine the proximate composition and bioactive properties of Australian native lemongrass (C. ambiguus). Antimicrobial capacity was carried out using the well diffusion method, antioxidant capacity by the FRAP method, and antidiabetic capacity by using the α-glucosidase inhibitory activity assay. The results obtained in the current study were compared with previously published literature on lemongrass (C. citratus). The results showed that C. ambiguus has lower fat and protein content and lower antioxidant and antimicrobial capacities than C. citratus, but it is very rich in fibre (67.55%) and has strong α-glucosidase inhibitory capacity. The total phenolic and total flavonoid content determined in the aqueous extract of C. ambiguus are also notable. The results of the present study showed that Australian native lemongrass has promising bioactive potential to be used as an alternative native herbal tea. [ABSTRACT FROM AUTHOR]

Objective: This study determined the effect of herbal drinks (Moringa, turmeric, and lemongrass) on uterine involution in post-partum women at the Tuminting Regional Health Center, Manado City. Materials and methods: This experiment used a "posttest control group design." The experimental group was given special treatment, including Moringa, Turmeric, and Lemongrass herbal drinks or Saraba Keke in sachet powder drinks by pouring hot water 150 ml/cc twice daily for 14 days. Meanwhile, the control group was given treatment as usual (conventional). After being given treatment, both groups had their uterine fundus height measured on day 3, day 7, and day 14, and then compared. For data analysis in this work, the paired t-test used. Results: The age of respondents in the intervention group was 20-35 years, which was 95%. Most respondents were parity 2, about 46% in the control group. The average uterine fundus height of post-partum mothers on day 7 in the intervention group was 5.1940, while in the control group, it was 6.019; and on day 14 in the intervention group, the height of the uterine fundus was no longer palpable, while in the control group, it was still 0.4460. Conclusion: A mixed herbal drink of Moringa, Turmeric, and Lemongrass was effective in accelerating uterine involution in post-partum women and no side effects were reported during the study. [ABSTRACT FROM AUTHOR]

Consumers are more interested in replacing commonly used chemical preservatives with natural substances. The effect of 5, 10, 20, 40, and 80 mg of thyme and lemongrass essential oils (THY and LMG, respectively) per 100 g of wheat flour was studied from the viewpoints of dough rheology, dough leavening progress, and the results of laboratory baking trial. Changes in dough viscoelastic properties were evaluated by the Mixolab rheometer and the company software. The higher the thyme oil portion, the higher the dough structure destruction by kneading and heat input in torque point C2, and consecutively shorter stability of dough consistency (up to one-half of the values recorded for the control); reversely, the LMG did not affect both features verifiably. In the 90 min leavening test, a dough weight loss was decelerated by both essential oils similarly. During the baking test, the average volume of wheat small breads as control was evaluated on level 167 mL (bread yield 451 mL/100 g flour). Independently of the dose of the THY or LMG, small bread volumes oscillated between 148–168 and 135–161 mL (average bread yields 442 and 443 mL/100 g flour, respectively). The shelf life of the products with a higher portion of essential oil was extended by up to 7 days. [ABSTRACT FROM AUTHOR]

The aromatic, flavorful, and fragrant qualities of lemongrass (Cymbopogon citratus) make it a valuable herb for culinary and medical uses. This study investigated the sensory quality of C. citratus and its richness in chemical compounds. The sensory tests were realized on the infusion of the plant and compared to 4 Teas among a group of participants. Further, total polyphenol, flavonoid, and tannin contents, and antioxidant activity were evaluated in the infusions of tested products. The primary metabolites in the dry matter of the plant were also measured. Before beginning, each participant was required to read and fill out an informed permission form by the national legislation and the Declaration of Helsinki's Ethical Principles for Medical Research Involving Human Subjects. Participants were aged between 20 and 60 years old with dominance of 20-39 years old (80%). Moreover, 60% of participants were women compared to 40% of men. Half the participants drink aromatized tea, mainly menthe. The infusion of C. citratus had the highest rating, calculated at 9.33±0.62 compared to the other aromas. The infusion of C. citratus was distinguished by its strong flavour and order, as well as its pleasant aroma, flavor, and aftertaste. The values of TPC, TFC, and tannin were 0.463 mg eq Q/g dry matter, 0.033 mg eq Q/g dry matter, and 21.96 mg eq AT /g dry matter, respectively. The antioxidant activity was 98.07%. The total sugar was the most dominant in the infusion of the plant with 0.376 g per g of dry matter of C. citratus, followed by lipids with 0.106 g per g of dry matter. This study presents new data on the nutritional value of C. citratus in Morocco and is suggested to be a reference for future research. [ABSTRACT FROM AUTHOR]

Cymbopogon citratus (C. citratus) has antioxidant, anti-inflammatory, and chemoprotective properties. This study investigated if increased doses of C. citratus ethanol extract can cause any serious adverse effect (gross, histological, hormonal, and/or inflammatory responses) on the testes of male albino rats. The sixty albino rats used in this study were randomly assigned into four groups of 15 each: 1/CENT each allowed free access to potable water, 2/CET2, 3/CET5, and 4/CET10 rats were each administered 250, 500 and 1000mg/kg b.w dose of C. citratus, respectively. At two-weeks intervals, the rats were examined for signs and lesions, serum samples were collected on days 0, 14 and 28 post-treatment and assayed for testosterone, luteinizing and follicle-stimulating hormones. On days 14 and 28 post treatment, gross and microscopic lesions were not observed in the testis of groups 2/CET2 and 3/CET5. Grossly, the testis of group 4/CET10 appeared reduced in size, with translucency of the visceral and parietal coats of the tunica vaginalis, while the histopathologic changes were degeneration of spermatogenic cells and fibrinous exudates expanding the intertubular spaces. Groups 2/CET2 and 3/CET5 testosterone, luteinizing and follicle stimulating hormones levels were significantly (p < 0.05) higher than that of 4/CET10. These findings suggest that degeneration of spermatogenic cells and fibrinous exudates expanding the intertubular spaces, and decreased reproductive hormone levels are diagnostic features of C. citratus toxicity in male albino rats. [ABSTRACT FROM AUTHOR]

Background: Lemongrass (LG) had various phytochemical components such as saponins, phenols, resins, alkaloids, tannins, flavonoids, glycosides and terpenes, minerals as well as vitamin C which had various pharmacological actions (antioxidant, anti-inflammatory, antibacterial, antibiotic, and antifungal) and growth promoter. The use of LG in broiler nutrition can be optimized the bird performance and gut health. Based on the high nutrition value of LG and absence of sufficient studies on the effect of lemongrass aqueous extract (LGX) on broiler performance and gut health (antioxidant and immune biomarkers and intestinal morphology), the aim of the present study is to investigate the impact of using LGX on productive performance, blood biomarkers, immunity and gut health of broilers. Methods: A total of two hundreds one-day- old male broiler chicks (Cobb 500) were fed on the starter basal diet for 6 days. From day 7 of age onwards, the birds were distributed, at random, into 4 groups. Each group included 5 replicates with 10 chicks per replicate. The birds in group 1 were not administered lemongrass extract (control, LGX0) while chicks in group 2 (LGX100), 3 (LGX200) and 4 (LGX300) were administered the aqueous extract of lemongrass in drinking water at levels of 100, 200 and 300 ml/l, respectively. The experimental period lasted for 35 day. Growth performance parameters, economic efficiency, hematological and biochemical biomarkers, expression of some antioxidant and immune related genes, cecal bacterial counts and intestinal morphological changes all were assessed. Results: The results indicated that, administration of LGX in drinking water at levels of 200, 100 ml/l, respectively significantly improved (p ≤ 0.001) body weight (BW), body weight gain (BWG) and feed conversion ratio (FCR) than the control but without any effect on economic efficiency index (EEI) and feed intake (FI). On the other hand, the addition of LGX in drinking water at levels of 300 ml/l significantly decreased (p ≤ 0.001) FI, EEI and all growth performance parameters as compared to those other groups. LGX supplemented birds groups exhibited higher Hb, PCV, MCH, platelets, and lymphocytes than the control group. However, the ratio of H/L in LGX100 and LGX200 groups was lower (p ≤ 0.001) than other groups. LGX supplemented groups showed low (p ≤ 0.001) cholesterol, creatinine, MDA and high (p ≤ 0.01) TAC. Up regulation (p ≤ 0.001) of the expressions of catalase, GPX1, and SOD1 were in LGX200 group compared to other groups. While, the proinflammatory genes expression (IL1B, IL6, IFNᵧ, and TNF) were down regulated (p ≤ 0.001) in the LGX200 compared to others. Moreover, LGX200 and LGX300 reduced (p ≤ 0.001) the intestinal pathogens counts (E.coli and Salmonella). Administration of LGX at levels of 200 and 100 ml/l, respectively enhanced (p ≤ 0.001) villi height and crypts depth. Conclusions: It was concluded that lemongrass aqueous extract can be included at level 100 and 200 ml/l in broilersˈ drinking water since it resulted in improved weight, feed conversion ratio, blood parameters, immunity and gut health without any deleterious effect on the health and performance of the birds. LGX at a 200 ml/l supplementation level achieved the best results followed by a 100 ml/l level. Also, the tested supplements can be used as natural growth promoter instead of antibiotic and help in solving the global problem of antimicrobial resistant bacterial strains responsible for human and animal diseases.

Air is an important part of life. The Quality of air can be seen from three parameters, namely chemical, physical, and biological parameters. One of the biological parameters of air quality can be seen from the presence of microorganisms such as bacteria. The presence of airborne microorganisms, especially in enclosed spaces can affect human health, one of which can cause respiratory infections. On the other hand, people often use air freshener products. Therefore, this research was conducted with the aim of analyzing the potential of air freshener based on siwalan (Borasus flabellifer) coir extract and lemongrass as antibacterial against airborne bacteria. This research is an experimental research with a pre test-post test design. The data obtained is in the form of the value of the Total Plate Count of indoor air bacteria before and after treatment using air freshener based on coir extract of siwalan and lemongrass. The results showed that both formulations (P1 and P2) of air freshener based on coir extract and lemongrass were able to reduce the number of airborne bacterial colonies in the room, with a significance value of 0.000

Background and Aim: The use of bioengineered nanocomposites as antimicrobials has increased in recent years, but very few investigations have been conducted to test their effectiveness against Pseudomonas aeruginosa, a pathogen presenting public health risks that can impact both humans and animals. The aim of this study was to assess the antimicrobial potential of phytofabricated silver nanoparticles synthesized using lemongrass extract against clinical isolates of P. aeruginosa. Materials and Methods: The extraction of active compounds from the leaves of Cymbopogon citratus was performed using ethanol (80%) as a solvent, high-performance liquid chromatography-mass spectrometry was used to analyze the chemical composition of the extract, the synthesis of silver nanoparticles (AgNPs) was done using silver nitrate (AgNO3) as a precursor, and the antimicrobial and antibiofilm activity of the extract and the AgNPs phytofabricated was assessed against 10 clinical strains of P. aeruginosa. Results: Lemongrass extract was found to consist of the following main compounds: Caffeic acid (445.21 ± 32.77 μg/g), p-coumaric acid (393.32 ± 39.56 μg/g), chlorogenic acid (377.65 ± 4.26 μg/g), quinic acid (161.52 ± 17.62 μg/g), and quercetin-3-glucoside (151.35 ± 11.34 μg/g). AgNPs were successfully phytofabricated using 2.5 mM AgNO3. The ultraviolet (UV)-visible absorption spectra of the AgNPs showed a localized surface plasmon resonance at 464 nm with an absorbance of 0.32 A. The 50x hydrodynamic diameter was 50.29 nm with a surface area value of 120.10 m2/ cm3, and the volume mean diameter and Sauter mean diameter were 50.63 nm and 49.96 nm, respectively. Despite the compound found in lemongrass extract, no antimicrobial activity was observed with the extract, while AgNPs exhibited noteworthy dose-dependent antimicrobial activity with inhibition diameters up to 24 mm and minimum inhibitory concentration (MIC) and minimum bactericidal concentration ranging from 2 to 16 and 4–64 µg/mL, respectively. AgNPs also demonstrated significant antibiofilm activity by inhibiting biofilms up to 99% between MIC/2 and 2MIC. Conclusion: The present study suggests that lemongrass is a good candidate for the synthesis of AgNPs with good physicochemical characteristics and having a strong anti-pseudomonas activity. Further research is needed to assess the stability and safety of these AgNPs.

Citral-rich lemongrass (Cymbopogon citratus) essential oils have been reported to exhibit anticancer, antimicrobial, antifungal, and antioxidant properties, leading to wide applications in the food industry, pharmaceuticals, cosmetics, perfumery markets, and pest inhibition. Vietnam has abundant lemongrass, which can be centralized to develop a lemongrass-based essential oil industry. In this work, essential oils were extracted from lemongrass harvested from the Bac Giang and Phu Yen provinces of Vietnam. The study aimed to investigate the influential factors, including distillation time, raw material size, water quality, and water-to-material ratio, to optimize oil production from C. citratus in a 10 L hydro-distillation boiler. The maximum oil yield was obtained under the optimal conditions: distillation time of 180 min, the plant material size of 2–3 mm, reverse osmosis (RO) and/or distilled water, and water-to-material ratio of 5/1 (mL/g). The maximum yields of lemongrass oil from Bac Giang and Phu Yen provinces were determined as 2.55 and 3.96 mL/kg, with corresponding citral contents analyzed of 45.5 and 68.1%, respectively. From the experimental data, a protocol for essential oil production from Vietnam’s C. citratus by the hydro-distillation technique was proposed.

Objective: This research aimed to assess the effects of dried plantain herb, lemongrass, and their combination on milk yield, immunological, liver enzymatic, serum, and milk mineral status in dairy cows. Materials and Methods: Twenty cows were arbitrarily assigned to 4 diets. Cows were given a basal ration considered as control diet (CL-D) having 14.93% crude protein (CP)and 10.96 MJ ME per kg dry matter (DM). Each cow was given 100 gm plantain, 100 gm lemongrass, and 50 gm plantain + 50 gm lemongrass with CL-D and taken as plantain diet (PT-D), lemongrass diet (LG-D), and plantain-lemongrass diet (PL-D), daily for 63 days, respectively. Blood and milk samples were taken four times at an interval of 14 days. Data were analyzed using a two-way repeated measures analysis of covariance. Results: Better DM consumption and milk yield were observed in the PT-D and LG-D compared to the CL-D (p ≤ 0.05). LG-D improved the milk's total solids, protein, and fat compared to CL-D (p < 0.05). Substantially, herbal groups improved serum albumin and reduced globulin concentrations compared to CL-D. LG-D had the highest serum immunoglobulin G, while herbal groups effectively reduced the liver enzymes compared to CL-D. Herbal groups did not affect serum and milk's calcium and phosphorus concentrations, while LG-D and PL-D substantially improved serum and milk zinc concentrations. Conclusions: Both plantain and lemongrass improved dairy cows' DM consumption and milk yield. Plantain and/or lemongrass enhanced the immune system and liver health, but not serum and milk calcium and phosphorus level. Lemongrass and a combination of plantain and lemongrass increased the serum and milk zinc concentrations. [J Adv Vet Anim Res 2024; 11(1.000): 185-193]

Fusarium oxysporum f.sp. conglutinans (FOC) causes yellow wilt, a serious cabbage disease. This fungal species is composed of many strains within two pathotypes. In this study, the inhibitory effect of essential oils of six aromatic and medicinal plants (oregano, thyme, clove, cinnamon, lemongrass, litsea) at multiple concentrations on eight FOC isolates of different origins was investigated. Our results showed that oregano essential oil had the greatest inhibitory effect. The tested essential oils could be divided into three clusters according to the similar content of their main components (oregano and thyme, clove and cinnamon, lemongrass, and litsea), which correlated to some extent with their inhibitory effects on FOC. The tested isolates also differed in their response to the studied essential oils. The differences within FOC races were more important than between races, and the sensitivity of isolates against essential oils seemed to be more related to the geographical origin of the isolates, rather than to their race affiliation. [ABSTRACT FROM AUTHOR]

Background and aims: Surface protection of coalmine overburden (OB) dumps against shallow slope failures can be achieved through a cost-effective and sustainable solution, i.e., vegetative cover. Hence, a small-scale study is needed to study the survival, growth performance, and root distribution of plants prior to conducting a pilot-scale study. Methods: In the present study, three plant species, Dendrocalamus strictus (Bamboo grass), Cymbopogon citratus (Lemon grass), and Chrysopogon zizanioides (Vetiver grass), an endemic grass species, are studied for the slope stabilization potential of OB dumps in Jharkhand, India. Growth characteristics and root architecture of grasses grown for five months under six varying proportions (treatments) of overburden, cow dung, and garden soil were studied. Results: It was found that Vetiver grass had the highest root area ratio, followed by Bamboo grass and Lemon grass. The maximum root cohesion was found to be 1 kPa for Bamboo grass, 7.33 kPa for Lemon grass, and 9.24 kPa for Vetiver grass among different treatments. Conclusions: Treatment condition with 80% overburden and 20% cow dung was suggested to be the best soil mix for optimum growth of the chosen grasses, which have the potential to mitigate surficial movement of soil mass on the overburden dumps through root reinforcement. [ABSTRACT FROM AUTHOR]

Citrus canker is a disease caused by the gram-negative bacterium Xanthomonas citri subp. citri (X. citri), which affects all commercially important varieties of citrus and can lead to significant losses. Fruit sanitization with products such as chlorine-based ones can reduce the spread of the disease. While effective, their use raises concerns about safety of the workers. This work proposes essential oils (EOs) as viable alternatives for fruit sanitization. EOs from Cymbopogon species were evaluated as to their antibacterial activity, their effect on the bacterial membrane, and their ability to sanitize citrus fruit. The in vitro assays revealed that the EOs from C. schoenanthus and C. citratus had a lower bactericidal concentration at 312 mg L−1, followed by 625 mg L−1 for C. martini and C. winterianus. Microscopy assay revealed that the bacterial cell membranes were disrupted after 15 min of contact with all EOs tested. Regarding the sanitizing potential, the EOs with higher proportions of geraniol were more effective in sanitizing acid limes. Fruit treated with C. shoenanthus and C. martini showed a reduction of ∼68% in the recovery of viable bacterial cells. Therefore, these EOs can be used as viable natural alternatives in citrus fruit disinfection. [ABSTRACT FROM AUTHOR]

Simple Summary: As whiteflies, Bemisia tabaci (Genn.), are continually developing resistance to conventional insecticides, it is crucial and pressing to seek new approaches to management. In this study, the synergistic effect of lemongrass essential oil and flometoquin, flonicamid, and sulfoxaflor on B. tabaci was studied. Based on the found LC values, the decreasing order of toxicity to B. tabaci was as follows: sulfoxaflor > flonicamid > flometoquin > lemongrass EO. Sulfoxaflor and flonicamid exhibited significant inhibition of AchE activity, while only flonicamid demonstrated a significant impact on α-esterase. However, none of the tested compounds affected cytochrome P450 or GST. Additionally, lemongrass EO and the tested insecticides exhibited significant binding affinity to AchE. The whitefly, Bemisia tabaci (Genn.), is one of the most dangerous polyphagous pests in the world. Eco-friendly compounds and new chemical insecticides have gained recognition for whitefly control. In this study, the toxicity and biochemical impact of flometoquin, flonicamid, and sulfoxaflor, alone or combined with lemongrass essential oil (EO), against B. tabaci was studied. In addition, a molecular docking study was conducted to assess the binding affinity of the tested compounds to AchE. Based on the LC values, the descending order of the toxicity of the tested compounds to B. tabaci adults was as follows: sulfoxaflor > flonicamid > flometoquin > lemongrass EO. The binary mixtures of each of the tested compounds with lemongrass EO exhibited synergism in all combinations, with observed mortalities ranging from 15.09 to 22.94% higher than expected for an additive effect. Sulfoxaflor and flonicamid, alone or in combination with lemongrass EO, significantly inhibited AchE activity while only flonicamid demonstrated a significant impact on α-esterase, and none of the tested compounds affected cytochrome P450 or GST. However, the specific activity of P450 was significantly inhibited by the lemongrass/sulfoxaflor mixture while α-esterase activity was significantly inhibited by the lemongrass/flometoquin mixture. Moreover, the lemongrass EO and all the tested insecticides exhibited significant binding affinity to AchE with energy scores ranging from −4.69 to −7.06 kcal/mol. The current findings provide a foundation for utilizing combinations of essential oils and insecticides in the integrated pest management (IPM) of B. tabaci. [ABSTRACT FROM AUTHOR]

Aging and poor maintenance of wastewater treatment works affect the wastewater quality and quantity. The focus of this experimental study was to identify appropriate grass which can be used for wastewater treatment in constructed wetlands. Vetiver grass (Chrysopogon zizanioides), Lemon grass (Cymbopogon flexuosus) and elephant grass (Pennisetum purpurreum) were planted separately and exposed to influent for a period of twenty weeks. Growth measurements were recorded weekly and the quality of the effluents from each of these grasses were assessed using standard methods to determine their treatment efficiency. The study demonstrates that different grass species can be used in constructed wetlands to treat wastewater effectively. Vetiver grass showed promising results in terms of treatment efficiency, meeting WHO standards for various water quality parameters. Further research and monitoring may be needed to confirm the long-term sustainability and performance of these grasses in wastewater treatment applications. [ABSTRACT FROM AUTHOR]

We present a groundbreaking achievement in essential oil extraction, showcasing the development of a highly efficient tri‐spiral condenser for use in microwave‐assisted extraction (MAE) setups. This innovative condenser is specifically designed to swiftly cool vapors, optimizing oil yield during the MAE process. Our investigations demonstrate a notable improvement in oil purity, attributed to the expedited condensation facilitated by the tri‐spiral design. Traditional heat exchangers, like the Liebig condenser, fall short in efficiently handling the high vapor production rates of MAE. In response to this challenge, our tri‐spiral condenser design enhances condensation speed, resulting in a remarkable 12.2% increase in oil yield compared with the Liebig condenser (2.1% with tri‐spiral vs. 1.87% with Liebig), as validated by Gas Chromatography–Mass Spectrometry. Beyond elevating oil purity, our design holds promise for diverse applications requiring rapid cooling. This work not only advances laboratory practices but also contributes to the broader societal need for efficient and sustainable methods in the essential oil extraction industry. Practical applications: The innovative tri‐spiral condenser design, as elucidated in this article, presents significant practical applications, especially in the realm of lemongrass essential oil recovery. This breakthrough technology holds promise for the fragrance and cosmetics industries, where lemongrass essential oil is a valuable ingredient. The novel condenser design enhances extraction efficiency, leading to a higher concentration of essential oil with improved aromatic compounds. Cosmetic manufacturers can benefit from a more potent and fragrant lemongrass oil for skincare and aromatherapy products. Additionally, the pharmaceutical sector could leverage this advancement for developing therapeutic products harnessing the medicinal properties of lemongrass. By optimizing essential oil recovery, the tri‐spiral condenser design not only caters to the needs of the fragrance, cosmetic, and pharmaceutical industries, but also aligns with a broader trend towards sustainable and efficient manufacturing practices, ensuring the accessibility of high‐quality lemongrass essential oil for diverse applications. [ABSTRACT FROM AUTHOR]

This study examined the concentrations of 40K, 238U, and 232Th radionuclides and evaluated the possible radiological health risks to medicinal plants found in Ewu, Edo State, Nigeria, using a NaI(Tl) gamma spectrometer. The six selected medicinal plants were Mangifera indica, Dacryodes edulis, Terminalia catappa, Cymbopogon citratus, Anacardium occidentale, and Persea Americana. The results showed that the activity concentrations for 40K ranged from 146.59 ± 4.81 in Persea americana to 296.08 3.42 Bq/kg in Cymbopogon citratus, with a mean of 209.43 ± 5.14 Bq/kg; 238U ranged from 2.25 ± 0.06 to 5.57 ± 0.15 Bq/kg, with a mean of 4.73 ± 0.15 Bq/kg; and 232Th varied from 4.50 ± 0.35 to 12.07 ± 0.57 Bq/kg, with a mean of 8.00 ± 0.40 Bq/kg. The maximum and minimum activity concentrations of both 238U and 232Th were found in Mangifera indica and Cymbopogon citratus, respectively. The calculated average committed effective dose ECED was 0.130 μSv/yr and the excess lifetime cancer risk (ELCR) has a mean of 0.00913 (x10-3). The radiological hazard assessment of the investigated medicinal plants was well within the internationally recommended safe limits of 0.3 mSv/yr and >10-4 for ECED and ELCR respectively. 232Th contributes 54.91% of the total ECED, while 238U contributes the least to 6.35%. 232Th exhibits a very strong, positive, and significant relationship with ECED and the ELCR, and it contributes largely to the ECED and ELCR due to ingestion of the examined herbal plant. Therefore, these medicinal plants are radiologically safe for human consumption. [ABSTRACT FROM AUTHOR]

Keeping an eye on the improvement of electron transportation, highly macroporous, stable, and the highly conductive carbon material is prepared from lemongrass, a known high cellulose content biomass. In order to further enhance its redox reaction and electron capture ability, the prepared biomass is further impregnated with a binary metal oxide, nickel tungsten oxide (NiWO 4). The physiochemical and morphological analyses confirmed the successful preparation of the anode electrocatalyst, and additionally, the electrochemical analysis revealed its higher oxidation reduction rate and stability. Importantly, the as prepared activated lemon grass carbon (ALC) and activated lemon grass carbon impregnated with NiWO 4 (ALC@NiWO 4) as anode catalyst based single chambered microbial fuel cell (SMFC) have yielded about 2.2 fold and 3.6 fold more power density compared to only carbon cloth (CC) anode based SMFC. The enhanced anode performance of these microporous nanocomposite modified anode is due to improved bacterial adhesion in the micro-pores, reduced charge transfer resistance, and improved capacitance in comparison to the coated CC. These findings have important implications for the development of high-performance SMFCs and the utilization of abundant biomass resources for energy generation. [Display omitted] • The potential of lemon grass with metal oxide as an anode catalyst is studied in a microbial fuel cell. • The synergistic effect of NiWO 4 and carbonized lemon grass-modified anode enhanced the power generation. • ALC@NiWO 4 exhibited the highest power density of 901.5 ± 42 mWm−2. • Higher biofilm formation was obtained from the surface of ALC@NiWO 4. [ABSTRACT FROM AUTHOR]

Objective: Themain objective of this research was to record ethnobotanical data on the use and exploitation of medicinal plants, highlighting their importance for the biodiversity, culture, and tradition of a peasant community in Venezuela. Sample/method: The study involved a population of 120 individuals, from which a sample size of 34 people was calculated using the formula for finite populations. A simple random sampling technique was employed, and all the participants were administered the TRAMIL (Traditions Medicine in Island) survey. Statistical analysis: The ethnopharmacological table was constructed, and descriptive statistics were used for analysis. Results: A total of 116 species of medicinal plants were documented to treat various health conditions. The informants reflected through their responses that they used medicinal plants in the first instance to address a health condition, employing varied forms of plant preparation, which include decoction (65.16%), raw consumption (16.77%), maceration (8.38%), and infusion (7.09%). The most commonly used plant parts are leaves, flowers, fruits, bark, peels, roots, and bulbs, while the most commonly used botanical families are Lamiaceae, Fabaceae, Rutaceae, Malvaceae, Verbenaceae, Acanthaceae, Asteraceae, and Euphorbaceae. On the other hand, the species with the highest TRAMIL Significant Use Level were Oregano orejón (Coleus amboinicus Lour.) (68.29), Malojillo [Cymbopogon citratus (D.C.) Stapf.] (60.97), Tua (Jatropha gossypiifolia L.) (34.15), Colombiana [Kalanchoe pinnata (Lam.) Pers.] (34.15), Poleo [Micromeria brownei (Sw.) Benth.] (29.27), Pasote (Chenopodium ambrosioides L.) (29.27), Llantén (Plantago major L.) (26.83), Te negro [Phyla stoechadifolia (L.) Small] (26.83), Yerbabuena (Mentha sp.) (21.85), and Curia (Justicia pectoralis Jacq.) (21.95). Conclusion: The community of El Onoto de El Valle de Tucutunemo, Aragua State, Venezuela has a notable utilization of medicinal plant species in their instance to treat different health conditions, with the predominant focus on treating flu and stomach ailments. It is important to emphasize that all individuals approached through various data collection instruments reported usingmedicinal plants, both individually and within their families, spanning a wide range of ages from children to the elderly. This reflects that the use of medicinal plants is part of their cultural heritage and ancestral roots. [ABSTRACT FROM AUTHOR]

Antimicrobial resistance (AMR), acknowledged by the World Health Organization (WHO) as a major global public health concern, refers to the ability of microorganisms to persist and proliferate despite the presence of antimicrobial agents. The misuse of these drugs has significantly contributed to the emergence of AMR in both human and animal populations. Microorganisms develop resistance through various mechanisms, necessitating innovative approaches to target these resistance mechanisms effectively. One such alternative is the utilization of essential oils (EOs), natural and volatile compounds produced by aromatic plants as secondary metabolites. Lemongrass oil (LGO) is among these essential oils, known for its broad spectrum of therapeutic properties, including antimicrobial, anticancer, antiinflammatory, and antioxidant activities. However, LGO is highly volatile and susceptible to degradation when exposed to environmental factors such as heat, humidity, light, and oxygen. Nanoencapsulation of LGO presents a promising solution to overcome these challenges, offering benefits such as enhanced stability, controlled release, and improved cellular uptake. This article focuses on the optimization and standardization of LGO-loaded chitosan and TPP nanoparticles through the process of ionic gelation. [ABSTRACT FROM AUTHOR]

Background & Objectives: Different essential oils (EOs) with antibacterial activities are promising natural sources for providing novel disinfectant agents for hospital surfaces. Materials & Methods: The component and antibacterial effects of six EOs, including Cuminum cyminum (CCEO), Artemisia sieberi (ASEO), Laurus nobilis (LNEO), Ferula gummosa (FGEO), Lippia citriodora (LCEO), and Cymbopogon citratus (CIEO) were assessed by GC-MS and 96-well micro-plates (IC50), against Staphylococcus aureus (S. aureus) ATCC 25923, Pseudomonas aeruginosa (P. aeruginosa) ATCC 27853 and clinical isolates of methicillin -resistant S. aureus (MRSA) and metallo-beta-lactamase (MBL)-producing P. aeruginosa. Then, the antibacterial effects of FGEO, the most effective EO, were evaluated on the trolley surface in a hospital for 1, 3, 5 and 10 min intervals. Results: CCEO, ASEO, and FGEO exerted the highest antibacterial activity against S. aureus, while CIEO and LNEO inferred the highest activity against P. aeruginosa. In addition, FGEO mitigated the growth of S. aureus and P. aeruginosa on the trolley surface (P<0.05). Conclusion: The studied EOs could be novel encouraging agents to develop further green antimicrobial agents against different infections. In addition, FGEO exhibited considerable antibacterial effects on the surface of the trolley. [ABSTRACT FROM AUTHOR]

A rare medicinal fungus called Taiwanofungus camphoratus gives people resistance to illness. In order to effectively obtain high−quality T. camphoratus mycelia, we added Cymbopogon citratus (lemongrass) water extract (LWE), which was prepared using hot water and dry lemongrass leaves and methyl jasmonate (MJ) as an additive, in order to cultivate T. camphoratus mycelia. The components of LWE were identified by gas chromatography–mass spectrometry as glucose (61.66%) and galactose (17.10%). Compare to the basal medium, 0.5–2.5 g·L−1 LWE and 5–25 μmol·L−1 MJ can enhance the proliferation of mycelia and the metabolism of polyunsaturated fatty acids (PUFAs). Among them, the T. camphoratus mycelia growth rate increased to 1.292 ± 0.01 cm·d−1 and 1.285 ± 0.05 cm·d−1, improving by 2.5 g·L−1 LWE and 25 μmol·L−1 MJ, respectively. PUFAs are mainly composed of linoleic acid (LA) and oleic acid (OA). The contents of LA and OA were 0.28 ± 0.02 mg·g−1 and 0.23 ± 0.05 mg·g−1 after MJ treatment, while the contents of LA and OA were 0.08 ± 0.03 mg·g−1 and 0.05 ± 0.05 mg·g−1 after LWE treatment. Transcriptome analyses revealed that 367 and 232 genes within MJ and LWE treatment were significantly different from the basal medium. Out of 13 unigenes, FAD2−2, SCD, and FAD2−1 had the highest expression levels according to the quantitative RT−PCR result. The bioinformatics analysis showed that three genes are closely related to the M8 chromosome of T. camphoratus, and they are hydrophobic transmembrane proteins. The identification and investigation of fatty acid genes in T. camphoratus mycelia will be improved by our findings. [ABSTRACT FROM AUTHOR]

Simple Summary: Essential oils (EOs) from many non-host plants have been reported to have insecticidal and antifeeding activities to larvae and oviposition deterrent effects on the female moths of Plutella xylostella. However, their effect on sex pheromone communication during mate location has been less studied. Here, we studied the antennal response of both adult sexes to seven non-host plant EOs and their inhibition effect on the sex pheromone orientation of males and the oviposition of female moths. The results demonstrated that 10 mg of calamus (Acorus gramineus) and citronella (Cymbopogon citratus) EOs reduced the attraction of synthetic sex pheromones to male moths up to 72% and 66% in a sensitive way. The calamus EO also decreased the egg-laying number of female moths on host plants, with the highest inhibition rate of 100%. These findings contribute to a better understanding of the roles of volatile plant secondary metabolites in modulating reproductive-related behaviors, and exploit EOs and plant resources that can be used for the behavioral control of P. xylostella. The pest management of Plutella xylostella, the global pest of cruciferous plants, is primarily dependent upon continued applications of insecticides, which has led to severe insecticide resistance and a series of ecological concerns. The essential oils (EOs) of non-host plants are considered to have a high application potential in pest behavioral control. In P. xylostella, the insecticidal properties, antifeeding activities, and oviposition inhibition effects of many EOs have been studied in larvae and female moths. However, less focus has been placed on the inhibitory effect on sex pheromone communication during courtship, which is vital for the reproduction of the offspring. In this study, by combining electrophysiological studies, laboratory behavioral assays, and field traps, we demonstrated that non-host plant EOs significantly inhibited the reproductive behaviors of both sexes. Notably, the calamus (Acorus gramineus) EO inhibited the preference of male moths for synthetic sex pheromone blends and reduced the egg-laying number of female moths on host plants, with the highest inhibition rates of 72% and 100%, respectively, suggesting a great application prospect of calamus and its EO on the behavioral control strategies of P. xylostella. [ABSTRACT FROM AUTHOR]

Lemongrass (Cymbopogon citratus) essential oil (LEO) and concentrations (1 and 1.5 %) of its nanoemulsion (LGNE) were included in beef burger formula to investigate their effect on shelf life, microbial, chemical indices, and technological properties. The product was frozen at -18±3ºC and examined at intervals (0, 48hrs, 4 weeks, then at a month interval for up to 4 months). GC analysis of LEO revealed that citric acid, verbenol, β-pinene, á-Myrcene and ethyl acetate formulate preponderance of the oil. FTIR, PDI, TEM and cytotoxicity were used for nanoemulsion characterization. The sensory attributes study disclosed that the raw oil drastically impacted the sensory criteria “colour and odour”; though samples with LGNE showed better acceptability. Samples with LEO showed the lowest TBC (P<0.05). Both LEO and LGNE showed great antibacterial effect against TCC. TYMC exhibited a numeral decrease in the count (P>0.05) except for the second month of storage (P<0.05). LGNE showed significant antioxidant efficacy nevertheless LEO samples showed higher TBARs values. TVBN was significant lower in LGNE samples especially in the last 2 months of storage. LGNE controlled the increase in samples pH compared to the control (P<0.05). WHC and cooking yield % showed improvement in the treatment’s samples. As well, diameter loss showed numeral decrease in treatments (P>0.05). In conclusion, LGNE generally improves the sensory and cooking properties of burger, over the LGO or the control samples. [ABSTRACT FROM AUTHOR]

Anthracnose caused by Colletotrichum lindemuthianum is one of the main diseases that affect the bean crop. The use of semisynthetic compounds for controlling anthracnose aims at providing a higher balance to the ecosystem and a lower environmental impact. Based on this context, the objective of this work was: a) to carry out the prospection of compounds such as Phenyl S Citral, Phenyl Se Citronellal, and Citral at concentrations of 1, 0.5, 0.25, 0.125, 0.0625, 0.0312, 0.0156, 0.0078, and 0.0039%, which were modified from the essential oil of citronella and lemongrass, for controlling C. lindemuthianum; b) to evaluate the initial performance of seedlings and treat the incidence of C. lindemuthianum in bean seeds with Phenyl S Citral and Phenyl Se Citronellal at concentrations of 0.125 and 0.0625%. Phenyl Se Citronellal at 0.5% controlled 100% of mycelial growth and Phenyl S Citral at 0.5 and 1% controlled more than 50% of mycelial growth of C. lindemuthianum. The treatment with Phenyl S Citral and Phenyl Se Citronellal did not affect the physiological quality of bean seeds while increasing seedling development when using the 0.0625% concentration of Phenyl Se Citronellal. Treatment with Phenyl Se Citronellal at both concentrations decreased the incidence of C. lindemuthianum infection. [ABSTRACT FROM AUTHOR]

This study aimed to investigate the phytochemistry of lemongrass (Cymbopogon citratus) inoculated with Azospirillum brasilense and grown in lead (Pb)-contaminated soil to assess its responses to inoculation under different Pb levels. The experimental design was completely randomized in a 2 × 5 factorial scheme: two levels of A. brasilense (absence or presence) and five Pb levels. After four months of treatment, the following were analyzed: total and reducing sugars, total phenolic content, flavonoids, antioxidant activity, antioxidant enzymes, proline, and essential oil (EO) content and composition. Soil Pb levels and A. brasilense inoculation affected phytochemicals in lemongrass plants. Azospirillum inoculation reduced total sugars in the roots at all soil Pb levels, while increasing Pb levels favored a rise in sugar contents. There was an increase in flavonoid content in treatments associated with Pb and inoculated with A. brasilense. Antioxidant capacity was lower at lower Pb levels, regardless of bacterial inoculation. Enzymatic response was mainly affected by Pb concentrations between 50 and 100 mg kg−1 soil. EO content was influenced by soil Pb levels, with higher EO production at 500 mg Pb kg−1 soil and without A. brasilense inoculation. Overall, lemongrass cultivation in Pb-contaminated areas can be an alternative to phytoremediation and EO production for the industry. [ABSTRACT FROM AUTHOR]

Sodium benzoate is commonly used as a food preservative to prevent deterioration. According to USEPA standards, the allowable maximum for sodium benzoate in beverages is 0.15%, and 0.13% in fruit juice. A high sodium benzoate intake can induce a variety of illnesses in humans. The current study analyzes the influence of various sodium benzoate concentrations on final product quality. The product was tested to evaluate its physiochemical, proximate, and sensory properties. Acidity, pH, specific gravity, and antioxidant characteristics were identified. The product with the least amount of Na-benzoate performed better than the products with a higher amount of sodium benzoate. Products with 0.06 and 0.07% sodium benzoate were disliked due to a pungent aftertaste, whereas products with 0.05% sodium benzoate were preferred. In terms of physicochemical parameters, all treatments had similar brix, pH, acidity, specific gravity, and antioxidant values. The proximate analyses were also found same for all three treatments. [ABSTRACT FROM AUTHOR]

This article provides a thorough analysis of the pharmacological effects, extraction characteristics, composition, and industrial uses of lemongrass essential oil. Since ancient times, people have used lemongrass (Cymbopogon citratus) essential oil as a cure to enhance the body's immune system, treat fever and malaria, improve blood circulation, and stimulate digestion. Essential oils made from lemongrass are used in a wide variety of sectors Aromatherapy, the food and beverage business, the pharmaceutical industry, the fragrance and flavour industry, the agriculture and livestock sector, the cosmetic and soap industry and the household and domestic industry are just some of the many businesses that make extensive use of them. The process that is utilized to extract the lemongrass essential oil, in addition to the conditions in which it is manufactured, have a sizeable impact on the product's overall quality. Lemongrass essential oil can be extracted through a wide variety of processes, some of which include steam distillation, traditional solvent extraction, Soxhlet extraction, ultrasound-assisted extraction (UAE), hydro distillation (HD), microwave-assisted hydro distillation (MAHD), simultaneous distillation and extraction (SDE), solvent-free microwave extraction (SFME) and supercritical fluid extraction (SFE) with carbon dioxide. The essential oil of Citronella contains a wide variety of chemicals, including citronellyl, geraniol, elemol elemene, citral A, citral B, caryophyllene, citronellyl acetate, geranyl acetate and -caryophyllene oxide, to name just a few of them. Additionally, significant amounts of minerals such as iron, zinc, magnesium, sodium, potassium, calcium, manganese and phosphorus can be discovered in lemongrass oil. In terms of its physicochemical properties, lemongrass oil is notable for a number of characteristics, including percentage of citral, freezing point, moisture content, acid value, ester value, carbonyl value, specific gravity, optical rotation, refractive index and phenol content, to name a few. However, the exact chemical make-up of lemongrass essential oil can change depending on a variety of circumstances, such as the plant's cultivar, age, geographic origin, photoperiod, harvesting season, and photoperiod, as well as the agricultural operations and agricultural practices used to produce the oil. Lemongrass oil is therefore employed in many pharmaceutical businesses as an antidepressant, analgesic, antipyretic, antibacterial, antiseptic digestive and astringent due to the presence of diverse chemical components. [ABSTRACT FROM AUTHOR]

An indirect-type forced convection solar dryer implementing a phase-changing material (PCM) as the energy-storing medium was designed, fabricated, and investigated in this study. The effects of changing the mass flow rate on the valuable energy and thermal efficiencies were studied. The experimental results showed that the instantaneous and daily efficiencies of the indirect solar dryer (ISD) increased with the initial increase in mass flow rate, beyond which the change is not prominent both with and without using the PCM. The system consisted of a solar energy accumulator (solar air collector with a PCM cavity), a drying compartment, and a blower. The charging and discharging characteristics of the thermal energy storage unit were evaluated experimentally. It was found that after using PCM, drying air temperature was higher than ambient air temperature by 9–12 ℃ after sunset for 4 h. Using PCM accelerated the process by which Cymbopogon citratus was effectively dried between 42 and 59 °C of drying air. Energy and exergy analysis of the drying process was performed. The daily energy efficiency of the solar energy accumulator reached 35.8%, while the daily exergy efficiency reached 13.84%. The exergy efficiency of the drying chamber was in the range of 47–97%. A free energy source, a large reduction in drying time, a higher drying capacity, a decrease in mass losses, and improved product quality all contributed to the proposed solar dryer's high potential. [ABSTRACT FROM AUTHOR]

The hybrid solar smart dryer (HSSD) was established to dry medicinal and aromatic herbs that are sensitive to direct sunlight. This study explores the effectiveness of the HSSD as an indirect solar drying technique in drying lemongrass, thyme, marjoram, and lavender at different temperatures (30, 40, and 50 ± 2 °C), focusing on its ability to retain quality features. The total energy consumption was estimated by values of 27.72 kWh for lemongrass and 43.02 kWh for lavender using HSSD at 50 ± 2 °C. Generally, HSSD showed an improvement in the studied physicochemical quality parameters. The green color retention was with the parameters of 15.60 ± 0.89, −5.28 ± 1.92, −3.89 ± 1.86, and −5.89 ± 2.40 for lemongrass, thyme, marjoram, and lavender samples, respectively. The highest values of the lemon grass, thyme, marjoram, and lavender oil content (1.96, 1.73, 3.40, and 2.76%, respectively) were obtained when the aromatic herbs were dried using the HSSD at 40 °C. This study showcased the effectiveness of solar dryers in preserving the physicochemical properties of lemongrass, thyme, marjoram, and lavender during drying, which could be applied to other food products. HSSD is a promising energy-efficient method that can save 19–36% of energy consumption, reducing the carbon footprint of drying processes and delivering high-quality products. [ABSTRACT FROM AUTHOR]

Simple Summary: The housefly (Musca domestica) is a global vector of several pathogens and a nuisance to humans and animals. Repellents reduce the risk of housefly vector diseases. Green repellents from plant essential oils (EOs) and their active compounds were, at the time, a promising option against houseflies. This study evaluated the housefly-repellent activities of every tested EO, their main constituents, and several combinations of them. The combinations were lemongrass (Cymbopogon citratus) EO + trans-anethole, lemongrass EO + star anise (Illicium verum) EO, geranial + trans-anethole, and star anise EO + geranial. The efficacies of every formulation were compared against each other and DEET (a synthetic repellent). All combinations were more effective in repellency than all single-component formulations and DEET. More significantly, all of them were safe for four non-target species: guppy (Poecilia reticulata), molly (Poecilia latipinna), dwarf honeybee (Apis florea), and stingless bee (Tetragonula pagdeni). These combinations could be developed into valuable green repellents for housefly vector disease management. The present study evaluated the housefly repellency of single-component formulations and combinations of lemongrass and star anise essential oils (EOs) and their main constituents. The efficacies of the combinations were compared against those of single-component formulations and DEET. Safety bioassays of all formulations and DEET on non-target species—guppy, molly, dwarf honeybee, and stingless bee—were conducted. GC–MS analysis showed that the main constituent of lemongrass EO was geranial (46.83%) and that of star anise EO was trans-anethole (92.88%). All combinations were highly synergistic compared to single-component formulations, with an increased repellent value (IR) of 34.6 to 51.2%. The greatest synergistic effect was achieved by 1.0% lemongrass EO + 1.0% trans-anethole combination, with an IR of 51.2%. The strongest, 100% repellent rate at 6 h was achieved by 1.0% geranial + 1.0% trans-anethole. They were twice as effective as DEET and caused obvious damage to housefly antennae under microscopic observation. All single-component formulations and combinations were benign to the four tested non-target species. In contrast, DEET was highly toxic to them. The synergistic repellency and biosafety of these two combinations are compellingly strong support for developing them into an effective green repellent. [ABSTRACT FROM AUTHOR]

Larvae of an invasive drain fly Clogmia albipunctata can survive in the drains of sinks in households and poses a risk to human health through the mechanical transmission of various multidrug-resistant bacteria and pathogens. We studied if the commercially available essential oils used for household cleaning could limit their survival in homes. We exposed third instar larvae from our own lab colony to 4 essential oils [Eucalyptus globulus L., Lavandula angustifolia L., Cymbopogon citratus L. and Citrus sinensis L. + Illicium verum L.] in 2.85, 5.7 and 8.55 µL/mL concentrations in mixture with water, 8 % acetic acid (vinegar) diluted in water (1:6) and 2 % DMSO. The lemongrass essential oil was most effective followed by eucalyptus, lavender and orange + badian essential oils. The efficacy of essential oils depended on the concentration, time of exposure and the carrier type. Drain fly larvae were very sensitive to even short time exposure to these essential oils which can help reduce their numbers in homes. [ABSTRACT FROM AUTHOR]

This study determined the in vitro trypanocidal activity of lemon grass(Cymbopogon citratus) methanolic extract against Trypanosoma brucei brucei and its bio infectivity in an in vivo study using male albino rat as the experimental animal. Five treatment levels were applied (0.002mg/ml, 0.02mg/ml, 0.2mg/ml, 2.0mg/ml and 0.0mg/ml). Trypanosome was inactive at all treatment levels of lemongrass. PCV was statistically the same at all exposure times whereas the control mice had significantly lower PCV than all treated mice apart from the control, all other treatments gave similar PCV results, but the highest grand mean value (51.87%) was obtained at 0.02mg/ml while week 4 recorded the highest value (51.53%). All treatment levels had equal effects on the mice's rectal temperature, but exposure time had significant effects on the temperature parameter where the grand mean temperature readings significantly varied from 35.55oC in week 2 to 37.47oC in week 5. The weight of mice varied significantly with both treatment (F=16.01, p<0.05) and time (F=5.10, p<0.05) factors. The highest mean weight recorded was 38.37±2.46g at 2.0mg/ml treatment in week 5 thus, the weight of mice increased as lemon grass treatment concentration and exposure time increased. Treatment concentrations significantly affected mice PCV and weight where the control level had the lowest values for these physical parameters. Also, exposure time significantly affected both the temperature and weight of mice. A general fluctuation but decline in feed intake was observed in mice at different treatment levels of methanolic extracts of lemon grass from weeks 1-7. A strong positive significant correlation exists between PCV and feed intake (r= -0.930, p<0.05) as well as between rectal temperature and weight of mice (r= -0.948, p<0.05). PCV and weight had a strong positive correlation though insignificant (r= 0.728, p>0.05). Therefore, lemon grass extract showed trypanocidal efficacy in the control of trypanosomiasis in Africa. [ABSTRACT FROM AUTHOR]

The aim of this study was to investigate the suitability of selected essential oils as more environmentally friendly, sustainable antimicrobial agents in photographic films for long‐term data storage. For this purpose, essential oils of oregano, thyme, and lemongrass were added in concentrations ranging from 0.4% to 4% to the gelatin comprising a protective layer for photographic microfilms. The emulsions were analyzed via UV‐Vis for their optical transparency, then tested for their antimicrobial efficacy against Bacillus subtilis, Staphylococcus hominis and Staphylococcus epidermidis. Thin coatings generated by spreading and drying the emulsions onto photographic film substrates were then characterized morphologically via SEM before and after standardized artificial aging procedures. It was found that oregano oil at a concentration of 0.4% maintained the optical and physical properties of the emulsion and proved highly effective against all tested bacteria. Thyme and lemongrass oil also exhibited some antimicrobial activity, however, were not able to inhibit the bacterial growth completely. At higher concentrations of the oils, the gelatin coatings cracked after artificial aging, which makes them unsuitable for the application. To conclude, selected essential oils like oregano oil can be used as an effective antimicrobial agent without compromising the quality of the photographic films. Practical Applications: The results of our research can be applied directly to the manufacturing of photographic films, specifically ones intended for long‐term data storage. Furthermore, many applications of gelatin or related materials which require antimicrobial activity, such as food packaging, could be enhanced by the addition of essential oils. [ABSTRACT FROM AUTHOR]

Compounds with insecticidal properties from plant essential oils are an alternative to manage Sitophilus zeamais. However, these compounds can affect physiological processes such as seed germination and plant growth. The objective was to evaluate the toxicity of the Cymbopogon flexuosus essential oil by fumigation to S. zeamais and its phytotoxic to Zea mays. The mortality of S. zeamais was 100% with the doses of 20, 30, 40 and 50 µL of the C. flexuosus essential oil per 20 g of grains. The lethal dose (LD50) of this essential oil was 9.3 µL and its lethal time (LT50) greater with the 10 µL (18.8 h) and lowest with that of 50 µL (0.02 h), respectively. The doses of 10 and 20 µL of the C. flexuosus essential oil did not reduce the seed germination and growth of corn plants. The essential oil of C. flexuosus is an alternative for the control of S. zeamais. [ABSTRACT FROM AUTHOR]

Objective: This research aimed to assess the effects of dried plantain herb, lemongrass, and their combination on milk yield, immunological, liver enzymatic, serum, and milk mineral status in dairy cows. Materials and Methods: Twenty cows were arbitrarily assigned to 4 diets. Cows were given a basal ration considered as control diet (CL-D) having 14.93% crude protein (CP)and 10.96 MJ ME per kg dry matter (DM). Each cow was given 100 gm plantain, 100 gm lemongrass, and 50 gm plantain + 50 gm lemongrass with CL-D and taken as plantain diet (PT-D), lemongrass diet (LG-D), and plantain-lemongrass diet (PL-D), daily for 63 days, respectively. Blood and milk samples were taken four times at an interval of 14 days. Data were analyzed using a two-way repeated measures analysis of covariance. Results: Better DM consumption and milk yield were observed in the PT-D and LG-D compared to the CL-D (p = 0.05). LG-D improved the milk's total solids, protein, and fat compared to CL-D (p < 0.05). Substantially, herbal groups improved serum albumin and reduced globulin concentrations compared to CL-D. LG-D had the highest serum immunoglobulin G, while herbal groups effectively reduced the liver enzymes compared to CL-D. Herbal groups did not affect serum and milk's calcium and phosphorus concentrations, while LG-D and PL-D substantially improved serum and milk zinc concentrations. Conclusions: Both plantain and lemongrass improved dairy cows' DM consumption and milk yield. Plantain and/or lemongrass enhanced the immune system and liver health, but not serum and milk calcium and phosphorus level. Lemongrass and a combination of plantain and lemongrass increased the serum and milk zinc concentrations. [ABSTRACT FROM AUTHOR]

Lemongrass essential oil (LEO), extracted from high-oil lemongrass, gains prominence as a versatile natural product due to growing demand for safe health solutions. LEO comprises beneficial compounds like citral, isoneral, geraniol, and citronellal, offering diverse pharmacological benefits such as antioxidant, antifungal, antibacterial, antiviral, and anticancer effects. LEO finds applications in food preservation, cosmetics, and pharmaceuticals, enhancing profitability across these sectors. The review focuses on the extraction of LEO, emphasizing the need for cost-effective methods. Ultrasound and supercritical fluid extraction are effective in reducing extraction time, increasing yields, and enhancing oil quality. LEO shows promise as a valuable natural resource across industries, with applications in packaging, coating, and film development. LEO's ability to extend the shelf life of food items and impart natural flavors positions it as a valuable asset. Overall, the review emphasizes LEO's therapeutic, antimicrobial, and antioxidant properties, strengthening its potential in the food, pharmaceutical, and cosmetic sectors.

This study aimed to analyze the formulation of an aromatherapy balm composed of the essential oils O. basilicum L. and C. citratus DC. Experimental research design involving the compilation of five balm formulations at concentrations of 0, 5, 10, 15, and 20%. For four months, balm formulations were made, and physical, chemical, and microbiological tests were carried out, which were further analyzed descriptively. The findings showed that the yield of essential oils obtained from distillation reached 0.19% w/v (O. basilicum L.) and 0.24% w/v (C. citratus DC). All formulations at concentrations of 0, 5, 10, 15, and 20% passed the organoleptic tests for topical dosage balms. This means that they were semisolid, smelled like essential oils, and were yellowish-brown based on the raw materials. The balm is completely homogeneous; there are no granules, and the color is evenly spread throughout the smear. The pH for the topical preparations ranged from 6–7, the dispersion power ranged from 5.02–6.10, and the adhesion ratio ranged from 2–6 depending on the concentration. Microbiological testing revealed that the aromatherapy balm was free of S. aureus and P. aeruginosa bacteria.