Search

Your search keyword '"Kilcoyne Michelle"' showing total 328 results
Start Over Author "Kilcoyne Michelle"
328 results on '"Kilcoyne Michelle"'

53. An insight into new glycotherapeutics in glial inflammation: Understanding the role of glycosylation in mitochondrial function and acute to the chronic phases of inflammation.

Author

Patil, Vaibhav, Bohara, Raghvendra, Winter, Carla, Kilcoyne, Michelle, McMahon, Siobhan, and Pandit, Abhay

Subjects
PEARSON correlation (Statistics), GLYCOSYLATION, LECTINS, NEUROGLIA, TISSUE remodeling, MITOCHONDRIA, GLYCANS
Abstract

Introduction: Glycosylation plays a critical role during inflammation and glial scar formation upon spinal cord injury (SCI) disease progression. Astrocytes and microglia are involved in this cascade to modulate the inflammation and tissue remodeling from acute to chronic phases. Therefore, understating the glycan changes in these glial cells is paramount. Method and results: A lectin microarray was undertaken using a cytokine‐driven inflammatory mixed glial culture model, revealing considerable differential glycosylation from the acute to the chronic phase in a cytokine‐combination generated inflamed MGC model. It was found that several N‐ and O‐linked glycans associated with glia during SCI were differentially regulated. Pearson's correlation hierarchical clustering showed that groups were separated into several clusters, illustrating the heterogenicity among the control, cytokine combination, and LPS treated groups and the day on which treatment was given. Control and LPS treatments were observed to be in dense clusters. This was further confirmed with lectin immunostaining in which GalNAc, GlcNAc, mannose, fucose and sialic acid‐binding residues were detected in astrocytes and microglia. However, the sialyltransferase inhibitor inhibited this modification (upregulation of the sialic acid expression), which indeed modulates the mitochondrial functions. Conclusions: The present study is the first functional investigation of glycosylation modulation in a mixed glial culture model, which elucidates the role of the glycome in neuroinflammation in progression and identified potential therapeutic targets for future glyco therapeutics in neuroinflammation. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

54. The milk fat globule membrane

Author

Ross, Sarah A., primary, Lane, Jonathan A., additional, Kilcoyne, Michelle, additional, Joshi, Lokesh, additional, and Hickey, Rita M., additional

Published
2015
Full Text
View/download PDF

56. A Self‐Powered Piezo‐Bioelectric Device Regulates Tendon Repair‐Associated Signaling Pathways through Modulation of Mechanosensitive Ion Channels (Adv. Mater. 40/2021)

Author

Fernandez‐Yague, Marc A., primary, Trotier, Alexandre, additional, Demir, Secil, additional, Abbah, Sunny Akogwu, additional, Larrañaga, Aitor, additional, Thirumaran, Arun, additional, Stapleton, Aimee, additional, Tofail, Syed A. M., additional, Palma, Matteo, additional, Kilcoyne, Michelle, additional, Pandit, Abhay, additional, and Biggs, Manus J., additional

Published
2021
Full Text
View/download PDF

57. Cross-platform comparison of glycan microarray formats

Author

Wang, Linlin, Cummings, Richard D, Smith, David F, Huflejt, Margaret, Campbell, Christopher T, Gildersleeve, Jeffrey C, Gerlach, Jared Q, Kilcoyne, Michelle, Joshi, Lokesh, Serna, Sonia, Reichardt, Niels-Christian, Parera Pera, Núria, Pieters, Roland J, Eng, William, and Mahal, Lara K

Published
2014
Full Text
View/download PDF

59. Analysis of the Neurotropic and Anti-inflammatory Effects of Neuroelectrode Functionalisation with a Heparan Sulphate Mimetic

Author

Vallejo-Giraldo, Catalina, primary, Mohand, Idir Ouidja, additional, Huynh, Minh Bao, additional, Trotier, Alexandre, additional, Krukiewicz, Katarzyna, additional, Pallipurath, Anuradha R, additional, Flannery, Andrea, additional, Kilcoyne, Michelle, additional, Pandit, Abhay, additional, Papy-Garcia, Dulce, additional, and Biggs, Manus Jonathan Paul, additional

Published
2021
Full Text
View/download PDF

62. Elastin-like recombinamers-based hydrogel modulates post-ischemic remodeling in a non-transmural myocardial infarction in sheep

Author

Contessotto, Paolo, primary, Orbanić, Doriana, additional, Da Costa, Mark, additional, Jin, Chunsheng, additional, Owens, Peter, additional, Chantepie, Sandrine, additional, Chinello, Clizia, additional, Newell, John, additional, Magni, Fulvio, additional, Papy-Garcia, Dulce, additional, Karlsson, Niclas G., additional, Kilcoyne, Michelle, additional, Dockery, Peter, additional, Rodríguez-Cabello, José C., additional, and Pandit, Abhay, additional

Published
2021
Full Text
View/download PDF

65. Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method modulate dendritic cell phenotype and activity

Author

Murphy, Anna, primary, Cwiklinski, Krystyna, additional, Lalor, Richard, additional, O’Connell, Barry, additional, Robinson, Mark W., additional, Gerlach, Jared, additional, Joshi, Lokesh, additional, Kilcoyne, Michelle, additional, Dalton, John P., additional, and O’Neill, Sandra M., additional

Published
2020
Full Text
View/download PDF

69. Laser induced periodic surface structures enhance neuroelectrode charge transfer capability and modulate astrocyte function in vitro

Author

Kelly, Adriona, Farid, Nazar, Krukiewicz, Katarzyna, Belisle, Nicole, Groarke, John, Waters, Elaine, Trotier, Alexandre, Laffir, Fathima R., Kilcoyne, Michelle, O'Connor, Gerard M., Biggs, Manus J., and SFI

Subjects
astrogliosis, neuroelectrode, electrochemical impedance, platinum/iridium, LIPSS cell alignment
Abstract

peer-reviewed The brain machine interface (BMI) describes a group of technologies capable of communicating with excitable nervous tissue within the central nervous system (CNS). BMI’s have seen major advances in recent years but these advances have been impeded due to a temporal deterioration in the signal to noise ratio of recording electrodes following insertion into the CNS. This deterioration has been attributed to an intrinsic host tissue response, namely reactive gliosis which involves a complex series of immune mediators resulting in implant encapsulation via the synthesis of proinflammatory signaling molecules and the recruitment of glial cells. There is a clinical need to reduce tissue encapsulation in situ and improve long-term neuroelectrode functionality. Physical modification of the electrode surface at the nanoscale could satisfy these requirements by integrating electrochemical and topographical signals to modulate neural cell behavior. In this study, commercially available platinum iridium (Pt/Ir) microelectrode probes were nanotopographically (NT) functionalized using femto/picosecond laser processing to generate laser induced periodic surface structures (LIPSS). Three different topographies and their physical properties were assessed by scanning electron microscopy and atomic force microscopy. The electrochemical properties of these interfaces were investigated using electrochemical impedance spectroscopy and cyclic voltammetry. The in vitro response of mixed cortical cultures (embryonic rat E14/E17), was subsequently assessed by confocal microscopy, ELISA and multiplex protein array analysis. Overall LIPSS features improved the electrochemical properties of the electrodes, promoted cell alignment and modulated the expression of multiple ion channels involved in key neuronal functions.

Published
2020

75. Surface molecules of extracellular vesicles secreted by the helminth pathogen Fasciola hepatica direct their internalisation by host cells

Author

de la Torre Escudero, Eduardo, Gerlach, Jared Q, Bennett, Adam, Cwiklinski, Krystyna, Jewhurst, Heather, Huson, Kathryn, Joshi, Lokesh, Kilcoyne, Michelle, O'Neill, Sandra M, Dalton, John, and Robinson, Mark

Subjects
lcsh:Arctic medicine. Tropical medicine, lcsh:RC955-962, lcsh:Public aspects of medicine, lcsh:RA1-1270
Abstract

Helminth parasites secrete extracellular vesicles (EVs) that can be internalised by host immune cells resulting in modulation of host immunity. While the molecular cargo of EVs have been characterised in many parasites, little is known about the surface-exposed molecules that participate in ligand-receptor interactions with the host cell surface to initiate vesicle docking and subsequent internalisation. Using a membrane-impermeable biotin reagent to capture proteins displayed on the outer membrane surface of two EV sub-populations (termed 15k and 120k EVs) released by adult F. hepatica, we describe 380 surface proteins including an array of virulence factors, membrane transport proteins and molecules involved in EV biogenesis/trafficking. Proteomics and immunohistochemical analysis show that the 120k EVs have an endosomal origin and may be released from the parasite via the protonephridial (excretory) system whilst the larger 15k EVs are released from the gastrodermal epithelial cells that line the fluke gut. A parallel lectin microarray strategy was used to profile the topology of major surface oligosaccharides of intact fluorogenically-labelled EVs as they would be displayed to the host. Lectin profiles corresponding to glycoconjugates exposed on the surface of the 15 K and 120K EV sub-populations are practically identical but are distinct from those of the parasite surface tegument, although all are predominated by high mannose sugars. We found that while the F. hepatica EVs were resistant to exo- and endo-glycosidases, the glyco-amidase PNGase F drastically remodelled the surface oligosaccharides and blocked the uptake of EVs by host macrophages. In contrast, pre-treatment with antibodies obtained from infected hosts, or purified antibodies raised against the extracellular domains of specific EV surface proteins (DM9-containing protein, CD63 receptor and myoferlin), significantly enhanced their cellular internalisation. This work highlights the diversity of EV biogenesis and trafficking pathways used by F. hepatica and sheds light on the molecular interaction between parasite EVs and host cells.

Published
2019
Full Text
View/download PDF

76. Laser-Induced Periodic Surface Structure Enhances Neuroelectrode Charge Transfer Capabilities and Modulates Astrocyte Function

Author

Kelly, Adriona, primary, Farid, Nazar, additional, Krukiewicz, Katarzyna, additional, Belisle, Nicole, additional, Groarke, John, additional, Waters, Elaine M., additional, Trotier, Alexandre, additional, Laffir, Fathima, additional, Kilcoyne, Michelle, additional, O’Connor, Gerard M., additional, and Biggs, Manus J., additional

Published
2020
Full Text
View/download PDF

82. Structure and N-acetylglucosamine binding of the distal domain of mouse adenovirus 2 fibre

Author

Singh, Abhimanyu K., primary, Nguyen, Thanh H., additional, Vidovszky, Márton Z., additional, Harrach, Balázs, additional, Benkő, Mária, additional, Kirwan, Alan, additional, Joshi, Lokesh, additional, Kilcoyne, Michelle, additional, Berbis, M. Álvaro, additional, Cañada, F. Javier, additional, Jiménez-Barbero, Jesús, additional, Menéndez, Margarita, additional, Wilson, Sarah S., additional, Bromme, Beth A., additional, Smith, Jason G., additional, and van Raaij, Mark J., additional

Published
2018
Full Text
View/download PDF

83. Binding of Helicobacter pylori to Human Gastric Mucins Correlates with Binding of TFF1

Author

Dunne, Ciara, primary, Naughton, Julie, additional, Duggan, Gina, additional, Loughrey, Catherine, additional, Kilcoyne, Michelle, additional, Joshi, Lokesh, additional, Carrington, Stephen, additional, Earley, Helen, additional, Backert, Steffen, additional, Robbe Masselot, Catherine, additional, May, Felicity, additional, and Clyne, Marguerite, additional

Published
2018
Full Text
View/download PDF

85. Neural Interface Materials: Preparation of Cytocompatible ITO Neuroelectrodes with Enhanced Electrochemical Characteristics Using a Facile Anodic Oxidation Process (Adv. Funct. Mater. 12/2018)

Author

Vallejo‐Giraldo, Catalina, primary, Pampaloni, Niccolò Paolo, additional, Pallipurath, Anuradha R., additional, Mokarian‐Tabari, Parvaneh, additional, O'Connell, John, additional, Holmes, Justin D., additional, Trotier, Alexandre, additional, Krukiewicz, Katarzyna, additional, Orpella‐Aceret, Gemma, additional, Pugliese, Eugenia, additional, Ballerini, Laura, additional, Kilcoyne, Michelle, additional, Dowd, Eilís, additional, Quinlan, Leo R., additional, Pandit, Abhay, additional, Kavanagh, Paul, additional, and Biggs, Manus Jonathan Paul, additional

Published
2018
Full Text
View/download PDF

86. Structure and N-acetylglucosamine binding of the distal domain of mouse adenovirus 2 fibre

Author

Agencia Estatal de Investigación (España), Instituto de Salud Carlos III, European Commission, Hungarian Scientific Research Fund, Enterprise Ireland, National University of Ireland, University of Washington, U.S. Public Health Service, National Institute of Allergy and Infectious Diseases (US), National Institute of General Medical Sciences (US), Singh, Abhimanyu K., Nguyen, Thanh H., Vidovszky, Márton Z., Harrach, Balázs, Benko, Mária, Kirwan, Alan, Joshi, Lokesh, Kilcoyne, Michelle, Berbís, Manuel Álvaro, Cañada, F. Javier, Jiménez-Barbero, Jesús, Menéndez, Margarita, Wilson, Sarah S., Bromme, Beth A., Smith, Jason G., van Raaij, Mark J., Agencia Estatal de Investigación (España), Instituto de Salud Carlos III, European Commission, Hungarian Scientific Research Fund, Enterprise Ireland, National University of Ireland, University of Washington, U.S. Public Health Service, National Institute of Allergy and Infectious Diseases (US), National Institute of General Medical Sciences (US), Singh, Abhimanyu K., Nguyen, Thanh H., Vidovszky, Márton Z., Harrach, Balázs, Benko, Mária, Kirwan, Alan, Joshi, Lokesh, Kilcoyne, Michelle, Berbís, Manuel Álvaro, Cañada, F. Javier, Jiménez-Barbero, Jesús, Menéndez, Margarita, Wilson, Sarah S., Bromme, Beth A., Smith, Jason G., and van Raaij, Mark J.

Abstract

Murine adenovirus 2 (MAdV-2) infects cells of the mouse gastrointestinal tract. Like human adenoviruses, it is a member of the genus Mastadenovirus, family Adenoviridae. The MAdV-2 genome has a single fibre gene that expresses a 787 residue-long protein. Through analogy to other adenovirus fibre proteins, it is expected that the carboxy-terminal virus-distal head domain of the fibre is responsible for binding to the host cell, although the natural receptor is unknown. The putative head domain has little sequence identity to adenovirus fibres of known structure. In this report, we present high-resolution crystal structures of the carboxy-terminal part of the MAdV-2 fibre. The structures reveal a domain with the typical adenovirus fibre head topology and a domain containing two triple ß-spiral repeats of the shaft domain. Through glycan microarray profiling, saturation transfer difference nuclear magnetic resonance spectroscopy, isothermal titration calorimetry and site-directed mutagenesis, we show that the fibre specifically binds to the monosaccharide N-acetylglucosamine (GlcNAc). The crystal structure of the complex reveals that GlcNAc binds between the AB and CD loops at the top of each of the three monomers of the MAdV-2 fibre head. However, infection competition assays show that soluble GlcNAc monosaccharide and natural GlcNAc-containing polymers do not inhibit infection by MAdV-2. Furthermore, site-directed mutation of the GlcNAc-binding residues does not prevent the inhibition of infection by soluble fibre protein. On the other hand, we show that the MAdV-2 fibre protein binds GlcNAc-containing mucin glycans, which suggests that the MAdV-2 fibre protein may play a role in viral mucin penetration in the mouse gut.

Published
2018

87. Regulation of inflammation, neurotrophins, glycosylation, sensory nerve innervation and pain in intervertebral disc degeneration using an implantable hyaluronic acid hydrogel

Author

Tiernan David, Kilcoyne Michelle, Carroll Oliver, Joshi Lokesh, Finn David P, Abbah Sunny A, Mohd Isa Isma Liza, Srivastava Akshay, Owens Peter, Dockery Peter, and Pandit Abhay

Subjects
Pathology, medicine.medical_specialty, Histology, Glycosylation, biology, business.industry, Biomedical Engineering, Bioengineering, Intervertebral disc, Inflammation, Degeneration (medical), Anatomy, chemistry.chemical_compound, medicine.anatomical_structure, chemistry, Hyaluronic acid, medicine, biology.protein, medicine.symptom, business, Biotechnology, Sensory nerve, Neurotrophin
Published
2016
Full Text
View/download PDF

88. Towards an elastin-based hydrogel platform for the repair of myocardial infarction

Author

Dockery Peter, Joshi Lokesh, Flora Tatjana, Contessotto Paolo, Kilcoyne Michelle, Pandit Abhay, Da Costa Mark, Spelat Renza, Chaturvedi Ankit, Orbanic Doriana, Milcovich Gesmi, Mccartin Mike, Marsico Grazia, Amenitsch Heinz, Rodriguez-Cabello Jos Carlos, and Rackauskas Mindaugas

Subjects
medicine.medical_specialty, Histology, biology, business.industry, Biomedical Engineering, Bioengineering, medicine.disease, Internal medicine, medicine, biology.protein, Cardiology, Myocardial infarction, business, Elastin, Biotechnology
Published
2016
Full Text
View/download PDF

92. Preparation of Cytocompatible ITO Neuroelectrodes with Enhanced Electrochemical Characteristics Using a Facile Anodic Oxidation Process

Author

Vallejo‐Giraldo, Catalina, primary, Pampaloni, Niccolò Paolo, additional, Pallipurath, Anuradha R., additional, Mokarian‐Tabari, Parvaneh, additional, O'Connell, John, additional, Holmes, Justin D., additional, Trotier, Alexandre, additional, Krukiewicz, Katarzyna, additional, Orpella‐Aceret, Gemma, additional, Pugliese, Eugenia, additional, Ballerini, Laura, additional, Kilcoyne, Michelle, additional, Dowd, Eilís, additional, Quinlan, Leo R., additional, Pandit, Abhay, additional, Kavanagh, Paul, additional, and Biggs, Manus Jonathan Paul, additional

Published
2017
Full Text
View/download PDF

93. Glycosylation-Based Serum Biomarkers for Cancer Diagnostics and Prognostics

Author

Kirwan, Alan, Utratna, Marta, O’Dwyer, Michael E., Joshi, Lokesh, and Kilcoyne, Michelle

Subjects
Article Subject
Abstract

Cancer is the second most common cause of death in developed countries with approximately 14 million newly diagnosed individuals and over 6 million cancer-related deaths in 2012. Many cancers are discovered at a more advanced stage but better survival rates are correlated with earlier detection. Current clinically approved cancer biomarkers are most effective when applied to patients with widespread cancer. Single biomarkers with satisfactory sensitivity and specificity have not been identified for the most common cancers and some biomarkers are ineffective for the detection of early stage cancers. Thus, novel biomarkers with better diagnostic and prognostic performance are required. Aberrant protein glycosylation is well known hallmark of cancer and represents a promising source of potential biomarkers. Glycoproteins enter circulation from tissues or blood cells through active secretion or leakage and patient serum is an attractive option as a source for biomarkers from a clinical and diagnostic perspective. A plethora of technical approaches have been developed to address the challenges of glycosylation structure detection and determination. This review summarises currently utilised glycoprotein biomarkers and novel glycosylation-based biomarkers from the serum glycoproteome under investigation as cancer diagnostics and for monitoring and prognostics and includes details of recent high throughput and other emerging glycoanalytical techniques.

Published
2015
Full Text
View/download PDF

95. Preparation of Cytocompatible ITO Neuroelectrodes with Enhanced Electrochemical Characteristics Using a Facile Anodic Oxidation Process.

Author

Vallejo‐Giraldo, Catalina, Pampaloni, Niccolò Paolo, Pallipurath, Anuradha R., Mokarian‐Tabari, Parvaneh, O'Connell, John, Holmes, Justin D., Trotier, Alexandre, Krukiewicz, Katarzyna, Orpella‐Aceret, Gemma, Pugliese, Eugenia, Ballerini, Laura, Kilcoyne, Michelle, Dowd, Eilís, Quinlan, Leo R., Pandit, Abhay, Kavanagh, Paul, and Biggs, Manus Jonathan Paul

Subjects
NEURAL circuitry, INDIUM tin oxide, CURRENT density (Electromagnetism), ELECTROLYTIC oxidation, LIGHT transmission
Abstract

Abstract: Physicochemical modification of implantable electrode systems is recognized as a viable strategy to enhance tissue/electrode integration and electrode performance in situ. In this work, a bench‐top electrochemical process to formulate anodized indium tin oxide (ITO) films with altered roughness, conducting profiles, and thickness is explored. In addition, the influence of these anodized films on neural cell adhesion, proliferation, and function indicates that anodized ITO film cytocompatibility can be altered by varying the anodization current density. Furthermore, ITO‐anodized films formed with a current density of 0.4 mA cm−2 show important primary neural cell survival, modulation of glial scar formation, and promotion of neural network activity. [ABSTRACT FROM AUTHOR]

Published
2018
Full Text
View/download PDF

97. Construction of a Natural Mucin Microarray and Interrogation for Biologically Relevant Glyco-Epitopes

Author

Kilcoyne, Michelle, Gerlach, Jared Q., Kane, Marian, Joshi, Lokesh, and ~

Subjects
Host-pathogen interaction, Carbohydrate, Glycosylation, High throughput, Glycomics, Lectin
Abstract

Mucins are the principal components of mucus, and mucin glycosylation has important roles in defense, microbial adhesion, immunomodulation, inflammation, and cancer. Mucin expression and glycosylation are dynamic, responding to changes in local environment and disease. Potentially hundreds of heterogeneous glycans can substitute one mucin molecule, and it is difficult to identify biologically accessible glyco-epitopes. Thirty-seven mucins, from the reproductive and gastrointestinal (GI) tracts of six species (bovine, ovine, equine, porcine, chicken, and deer) and from two human-derived cell lines, were purified. Following optimization of mucin printing and construction of a novel mucin microarray, the glycoprofiles of the whole mucins on the microarray were compared using a panel of lectins and one antibody. Accessible glyco-motifs of GI mucins varied according to species and localization of mucin origin, with terminal fucose, the sialyl T-antigen, and N-linked oligosaccharides identified as potentially important. The occurrence of T- and sialyl T-antigen varied in bovine and ovine reproductive tract mucins, and terminal N-acetylgalactosamine (GalNAc) and sulfated carbohydrates were detected. This study introduces natural mucin microarrays as an effective tool for profiling mucin glyco-epitopes and highlights their potential for discovery of biologically important motifs in bacterial host interactions and fertility. peer-reviewed

Published
2012

99. A Preliminary Study Examining the Binding Capacity of Akkermansia muciniphila and Desulfovibrio spp., to Colonic Mucin in Health and Ulcerative Colitis

Author

Earley, Helen, primary, Lennon, Grainne, additional, Balfe, Aine, additional, Kilcoyne, Michelle, additional, Clyne, Marguerite, additional, Joshi, Lokesh, additional, Carrington, Stephen, additional, Martin, Sean T., additional, Coffey, J. Calvin, additional, Winter, Desmond C., additional, and O’Connell, P. Ronan, additional

Published
2015
Full Text
View/download PDF

100. Structure and Sialyllactose Binding of the Carboxy-Terminal Head Domain of the Fibre from a Siadenovirus, Turkey Adenovirus 3

Author

Singh, Abhimanyu K., primary, Berbís, M. Álvaro, additional, Ballmann, Mónika Z., additional, Kilcoyne, Michelle, additional, Menéndez, Margarita, additional, Nguyen, Thanh H., additional, Joshi, Lokesh, additional, Cañada, F. Javier, additional, Jiménez-Barbero, Jesús, additional, Benkő, Mária, additional, Harrach, Balázs, additional, and van Raaij, Mark J., additional

Published
2015
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results