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The effect of cobalt on the precipitation hardening behavior of the maraging stainless steels was studied. The hardness test indicated that cobalt addition could advance peak aging time and generate a higher peak hardness. The statistical results of nano-size precipitates distribution demonstrated that cobalt increased the density of precipitates, thus resulting in a stronger precipitation hardening contribution. Based on the first-principles calculations and atom probe tomography (APT) results, a possible mechanism has been proposed to reveal the underlying mechanism: cobalt promotes the nucleation of precipitates by decreasing the diffusion activation energy of Ni and Ti.

Obtaining monitoring global droplet size distribution (DSD) is of great scientific significance for the study of precipitation characteristics and has important applications in retrieving precipitation by precipitation radar. Based on the GPM data provided by NASA's Goddard Flight Center (GFSC) and ERA5 reanalysis data, three-dimensional structure of DPR-derived mass-weighted mean diameter (D0) and normalized intercept parameter (dBNw) of DSD and precipitation reflectivity factor (PRF) from June to August in 2014-2020 were investigated. The regional and sea-land differences of DSD parameters and PRF in different latitudinal zones were also analyzed. The results are as follows. The spatial distribution patterns of D0 and dBNw are different and the large value region of dBNw (> 35) has small D0 (< 1 mm), while the low value region of dBNw (< 30) has large D0 (> 1.2 mm). The mean D0 over the land surface is larger than that over the ocean, but the mean dBNw is smaller than that over the ocean, and there are great differences between land and sea in the tropics and the northern hemisphere. The vertical distribution of precipitation reflectivity factor (PRF) is mainly determined by D0, and dBNw is a secondary factor. The D0 mean profile indicates that the main microphysical processes of the large, medium, and small precipitation particles are different. The dBNw profile indicates that the concentration of mean particles in the upper layer is smaller than that in the lower layer. Compared with other terrestrial regions, the distributions of DSD parameters and PRF over the Tibetan Plateau are the most concentrated, and the precipitation particles are smaller in size and lower in concentration.

Abstract A Randall–Sundrum-like braneworld scenario is constructed in a 5D extension of the Lorentz-violating massive gravity. The gauge hierarchy problem is solved in current model. The linear perturbations are calculated, and it is found that the tensor and vector perturbations are robust and free from the ghost and tachyonic instabilities, however, the scalar perturbation is a ghost filed. After Kaluza–Klein reduction, all the tensor, vector and scalar modes are massive and possess the mass splitting of order of TeV in their respective mass spectra. The massive ground states of tensor and scalar modes propagate only along the brane, however, the vector ground state is absent in the mass spectrum. By introducing the Goldberger-Wise mechanism to stabilize the extra dimension, the 4D effective theory on the brane includes a nearly massless graviton plus three towers of very massive spin-2, spin-1 and ghost spin-0 particles.

As the most abundant group of mammals, rodents possess a very rich ecotype, which makes them ideal for studying the relationship between diet and host gut microecology. Zokors are specialized herbivorous rodents adapted to living underground. Unlike more generalized herbivorous rodents, they feed on the underground parts of grassland plants. There are two species of the genus Myospalax in the Eurasian steppes in China: one is Myospalax psilurus, which inhabits meadow grasslands and forest edge areas, and the other is M. aspalax, which inhabits typical grassland areas. How are the dietary choices of the two species adapted to long-term subterranean life, and what is the relationship of this diet with gut microbes? Are there unique indicator genera for their gut microbial communities? Relevant factors, such as the ability of both species to degrade cellulose, are not yet clear. In this study, we analyzed the gut bacterial communities and diet compositions of two species of zokors using 16S amplicon technology combined with macro-barcoding technology. We found that the diversity of gut microbial bacterial communities in M. psilurus was significantly higher than that in M. aspalax, and that the two species of zokors possessed different gut bacterial indicator genera. Differences in the feeding habits of the two species of zokors stem from food composition rather than diversity. Based on the results of Mantel analyses, the gut bacterial community of M. aspalax showed a significant positive correlation with the creeping-rooted type food, and there was a complementary relationship between the axis root-type-food- and the rhizome-type-food-dominated (containing bulb types and tuberous root types) food groups. Functional prediction based on KEGG found that M. psilurus possessed a stronger degradation ability in the same cellulose degradation pathway. Neutral modeling results show that the gut flora of the M. psilurus has a wider ecological niche compared to that of the M. aspalax. This provides a new perspective for understanding how rodents living underground in grassland areas respond to changes in food conditions.

Due to various typical unstructured factors in the environment of photovoltaic power stations, such as high feature similarity, weak textures, and simple structures, the motion model of the ORB-SLAM2 algorithm performs poorly, leading to a decline in tracking accuracy. To address this issue, we propose PE-SLAM, which improves the ORB-SLAM2 algorithm’s motion model by incorporating the particle swarm optimization algorithm combined with epipolar constraint to eliminate mismatches. First, a new mutation strategy is proposed to introduce perturbations to the pbest (personal best value) during the late convergence stage of the PSO algorithm, thereby preventing the PSO algorithm from falling into local optima. Then, the improved PSO algorithm is used to solve the fundamental matrix between two images based on the feature matching relationships obtained from the motion model. Finally, the epipolar constraint is applied using the computed fundamental matrix to eliminate incorrect matches produced by the motion model, thereby enhancing the tracking accuracy and robustness of the ORB-SLAM2 algorithm in unstructured photovoltaic power station scenarios. In feature matching experiments, compared to the ORB algorithm and the ORB+HAMMING algorithm, the ORB+PE-match algorithm achieved an average accuracy improvement of 19.5%, 14.0%, and 6.0% in unstructured environments, respectively, with better recall rates. In the trajectory experiments of the TUM dataset, PE-SLAM reduced the average absolute trajectory error compared to ORB-SLAM2 by 29.1% and the average relative pose error by 27.0%. In the photovoltaic power station scene mapping experiment, the dense point cloud map constructed has less overlap and is complete, reflecting that PE-SLAM has basically overcome the unstructured factors of the photovoltaic power station scene and is suitable for applications in this scene.

In angiosperms, seed size is a critical trait that is influenced by the complex interplay between the endosperm and seed coat. The HAIKU (IKU) pathway, involving the transcription factor WRKY10, plays a crucial role in regulating seed size in Arabidopsis thaliana. However, the downstream targets of WRKY10 and their roles in seed size determination remain largely unexplored. Here, we identified LACCASE2 (LAC2), a laccase gene involved in lignin biosynthesis, as a new downstream target of WRKY10. We observed that the expression of LAC2 was upregulated in the mini3 mutant, which is defective in WRKY10. We demonstrated that WRKY10 directly binds to the promoter of miR397a, activating its expression. miR397a, in turn, represses the expression of LAC2. Genetic analyses revealed that a mutation in LAC2 or overexpression of miR397a partially rescued the small seed phenotype of the MINISEED3 (MINI3) mutant mini3. Conversely, the overexpression of LAC2 in the wild type led to a decrease in seed size. These findings suggest that LAC2 functions as a negative regulator of seed size, and its expression is modulated by WRKY10 through miR397a. Our study uncovers a novel WRKY10-miR397a-LAC2 pathway that regulates seed size in Arabidopsis, providing new insights into the complex regulatory network governing seed development in plants.

A metal-free and Selectfluor-mediated selective oxidation reaction of benzo[d]isothiazol-3(2H)-ones in aqueous media is presented. This novel strategy provides a facile, green, and efficient approach to access important benzo[d]isothiazol-3(2H)-one-1-oxides with excellent yields and high tolerance to various functional groups. Furthermore, the purification of benzoisothiazol-3-one-1-oxides does not rely on column chromatography. Moreover, the preparation of saccharine derivatives has been achieved through sequential, double oxidation reactions in a one-pot aqueous media.

WO3 and silicone rubber (SR)-based gasochromic composites were fabricated to detect hydrogen leaks at room temperature. WO3 rod-like nanostructures were uniformly distributed in the SR matrix, with a particle size of 60–100 nm. The hydrogen permeability of these composites reached 1.77 cm3·cm/cm2·s·cmHg. At a 10% hydrogen concentration, the visible light reflectance of the composite decreased 49% during about 40 s, with a color change rate of 6.4% s−1. Moreover, the composite detected hydrogen concentrations as low as 0.1%. And a color scale was obtained for easily assessing hydrogen concentrations in the environment based on the color of composites. Finally, the composite materials as disposable sensors underwent testing at several Sinopec hydrogen refueling stations.

Landslide disasters frequently result in significant casualties and property losses, underscoring the critical importance of research on landslide displacement prediction. This paper introduces an approach combining improved empirical mode decomposition (ICEEMDAN) and singular entropy-enhanced singular spectrum analysis (SSA) to predict landslide displacement using a time series short-duration memory network (LSTM). Initially, ICEEMDAN decomposes the landslide displacement time series into trend and periodic terms. SSA is then employed to denoise these components before fitting the trend term with LSTM. Pearson correlation analysis is utilized to identify characteristic factors within the LSTM model, followed by predictions using a multivariate LSTM model. The empirical results from the Baijiabao landslide in the Three Gorges Reservoir area demonstrate that the joint ICEEMDAN-SSA approach, when combined with LSTM modeling, outperforms the separate applications of SSA and ICEEMDAN, as well as other models such as RNN and SVM. Specifically, the ICEEMDAN-SSA-LSTM model achieves an RMSE of 6.472 mm and an MAE of 4.992 mm, which are considerably lower than those of the RNN model (19.945 mm and 15.343 mm, respectively) and the SVM model (16.584 mm and 11.748 mm, respectively). Additionally, the R2 value for the ICEEMDAN-SSA-LSTM model is 97.5%, significantly higher than the RNN model’s 72.3% and the SVM model’s 92.8%. By summing the predictions of the trend and periodic terms, the cumulative displacement prediction is obtained, indicating the superior accuracy of the ICEEMDAN-SSA-LSTM model. This model provides a new benchmark for precise landslide displacement prediction and contributes valuable insights to related research.

Critical raw materials (CRMs) supply is a challenge that EU countries have to face, with many thinking about domestic procurement from natural ore deposits and anthropogenic deposits (landfills and extractive waste facilities). The present research focuses on the possibilities linked to the supply of CRMs and the potential for exploiting rare earth elements (REEs), investigating a large variety of extractive waste and siliceous rocks in the Piedmont region (Northern Italy). Indeed, the recovery of REEs from the extractive waste (EW) of siliceous quarries and other siliceous ore deposits can be a valuable way to reduce supply chain risks. Starting with a review of the literature on mining activities in Piedmont and continuing with the sampling and geochemical, mineralogical, petrographic, and environmental characterization of EW facilities connected to siliceous dimension stones, of kaolinitic gneiss ore deposits, and of soils present near the investigated areas, this study shows that the degree of REEs enrichment differs depending on the sampling area (soil or EW) and lithology. The concentration of REEs in the EW at some sampling sites fulfils the indicators of industrial-grade and industrial recovery; the high cumulative production and potential market values of EW and the positive recovery effects through proven methodologies indicate a viable prospect of REE recovery from EW. However, REE recovery industrialization faces challenges such as the difficulty in achieving efficient large-scale recovery due to large regional differences in REE abundance, the mismatch between potential market value and waste annual production, etc. Nonetheless, in the future, EW from dimension stone quarries could be differentially studied and reused based on the enrichment and distribution characteristics of trace elements. The present paper shows investigation procedures undertaken to determine both CRMs potentialities and environmental issues (on the basis of literature data employed to select the more-promising areas and on sampling and characterization activities in the selected areas), together with procedures to determine the waste quantities and tentative economic values of REEs present in the investigated areas. This approach, tested on a large area (Piedmont region), is replicable and applicable to other similar case studies (at EU and non-EU levels) and offers decision makers the possibility to acquire a general overview of the potential available resources in order to decide whether and where to concentrate efforts (including economic ones) in a more detailed study to evaluate the exploitable anthropogenic deposits.

This research centers on the necessity for synchronized management of natural resources in urban agglomerations. This study utilizes the ecosystem services theory to analyze the interplay between land use and the Food-Water-Energy (F-W-E) nexus in the Beijing-Tianjin-Hebei (B-T-H) region from 2000 to 2030. Assessment of ecosystem services is conducted using InVEST models, which include Habitat Quality (HQ), Water Yield (WY), Carbon Sequestration (CS), Soil Retention (SDR), and Food Production (FP). The findings indicate an annual increase in construction land with a concurrent notable decrease in cultivated land. Furthermore, HQ, CS, and per capita FP show an annual decline until 2020, which is expected to continue until 2030. Conversely, WY and SDR have been growing annually, albeit projected to decline by 2030. Spearman coefficient analysis uncovers synergies between HQ and CS, SDR and CS, and SDR and HQ, alongside trade-offs between CS and WY and HQ and WY. Trade-offs are also observed between FP and SDR, CS, and HQ. Applying K-means clustering analysis facilitates county-based spatial planning for the F-W-E system, providing crucial insights and suggestions for sustainable resource management.

The law of ground pressure behavior can accurately guide the material proportion and performance of the roadside backfill body (RBB) in gob-side entry retaining (GER), thereby reducing the waste of materials and the cost of retaining roadway. In this study, a similar material modeling is used to verify the spatiotemporal law of the ground pressure in the engineering case of solid dense backfilling mining in Xingtai Mine, China. Based on that law, the theoretical requirements for the bearing performance of the RBB are proposed. Finally, a material mix proportion that meets the theoretical requirements is obtained by compression test, and the deformation and failure characteristics of the backfill body with that mix proportion are analyzed. The results show that the maximum pressure of the backfill body measured in Xingtai Mine is 5.5 MPa, which is about 40 m away from the coal face; after 40 m, the force on the backfill body will not increase anymore. The physical simulation experiment also proved that the ground pressure behind the coal face increases gradually and tends to be during the backfilling process, which shows certain spatiotemporal characteristics. Through the proportioning experiment, it is determined that the optimal material mix proportion of the RBB is gangue:fly ash:cement = 10:3:1, which meets the theoretical requirement that the strength of the RBB at any position is not less than the ground pressure at that position. The research results provide theoretical support for the field practice of GER in solid dense backfilling mining.

By taking advantage of the braneworld sum rules, we explore the feasibility of constructing a flat 3-brane scenario consisting solely of positive tension branes in a 5D extension of the Lorentz-violating massive gravity. It is found that the theory supports three distinct brane configurations, one of which is exactly what we expected, consisting solely of two positive tension branes. The cosmological problem of Randall-Sundrum-1 model and the gauge hierarchy problem can be solved in this model simultaneously. Furthermore, the analysis of linear perturbations reveals that the tensor, vector and scalar modes are all massive and share the same mass spectrum, except that the ground state of vector mode is absent. Moreover, the tensor and vector modes are robust, but the scalar mode is ghost-like. Interestingly, even though the Kaluza-Klein gravitons have an extremely small mass splitting scale, an estimation of the effective gravitational potential and production of these gravitons on the brane indicates that the phenomenology of the present model is equivalent to that of the 6D ADD model.

Relying on the middle line of south-to-north water diversion project ning storage reservoir has built the construction load impact assessment project, the study through the analysis of field conditions select reasonable monitoring section and monitoring scheme, the late monitoring, the results can reflect the overall deformation of the wall and stress, and use in advance in the wall, monitoring instruments, to the typical section of field deformation, pressure, strain and stress, automatic oblique monitoring results to introduce the stress strain of the whole wall. Research results: The soil pressure showed the larger on the outside and the slightly smaller on the inside; Concrete strain relative to the initial state in the direction of vertical wall and vertical strain increment is larger, The strain increment along the wall direction is small; The data of the reinforcement stress gauge are all negative, And the quantity value of the inner steel bar gauge continues to increase, The monitoring value of the outer steel bar gauge has a tendency to decrease gradually; Small displacement of the plastic concrete walls, Large displacement of reinforced concrete walls, And the value along the wall for the small law; The inclination displacement value of monitoring section 2-2 and 3-3 gradually decreases with the continuous construction, The displacement value of monitoring 1–1 section also becomes smaller in the first and middle stages of construction, But in the late construction, This shows that the horizontal displacement of the monitoring 1–1 section in the later construction period has a recovery trend; The top settlement value is larger at the 1st-3rd wall, About 6 mm–10mm, The 4–6 pin is smaller, About 4 mm–5mm, Article 7–10, the pin is directly crushed by the vehicle, Its value is approximately 6 mm–8mm.

Numerous subway projects are planned by China's city governments, and more subways can hardly avoid under-crossing rivers. While often being located in complex natural and social environments, subway shield construction under-crossing a river (SSCUR) is more susceptible to safety accidents, causing substantial casualties, and monetary losses. Therefore, there is an urgent need to investigate safety risks during SSCUR. The paper identified the safety risks during SSCUR by using a literature review and experts' evaluation, proposed a new safety risk assessment model by integrating confirmatory factor analysis (CFA) and fuzzy evidence reasoning (FER), and then selected a project to validate the feasibility of the proposed model. Research results show that (a) a safety risk list of SSCUR was identified, including 5 first-level safety risks and 38 second-level safety risks; (b) the proposed safety risk assessment model can be used to assess the safety risk of SSCUR; (c) safety inspection, safety organization and duty, quicksand layer, and high-pressure phreatic water were the high-level risks, and the onsite total safety risk was at the medium level; (d) management-type safety risks, environment-type safety risks, and personnel-type safety risks have higher expected utility values, and manager-type safety risks were expected have higher risk-utility values when compared to worker-type safety risks. The research can enrich the theoretical knowledge of SSCUR safety risk assessment and provide references to safety managers for conducting scientific and effective safety management on the construction site when a subway crosses under a river.

BackgroundCalcific aortic valve disease (CAVD) is one of the most prevalent valvular diseases and is the second most common cause for cardiac surgery. However, the mechanism of CAVD remains unclear. This study aimed to investigate the role of pyroptosis-related genes in CAVD by performing comprehensive bioinformatics analysis.MethodsThree microarray datasets (GSE51472, GSE12644 and GSE83453) and one RNA sequencing dataset (GSE153555) were obtained from the Gene Expression Omnibus (GEO) database. Pyroptosis-related differentially expressed genes (DEGs) were identified between the calcified and the normal valve samples. LASSO regression and random forest (RF) machine learning analyses were performed to identify pyroptosis-related DEGs with diagnostic value. A diagnostic model was constructed with the diagnostic candidate pyroptosis-related DEGs. Receiver operating characteristic (ROC) curve analysis was performed to estimate the diagnostic performances of the diagnostic model and the individual diagnostic candidate genes in the training and validation cohorts. CIBERSORT analysis was performed to estimate the differences in the infiltration of the immune cell types. Pearson correlation analysis was used to investigate associations between the diagnostic biomarkers and the immune cell types. Immunohistochemistry was used to validate protein concentration.ResultsWe identified 805 DEGs, including 319 down-regulated genes and 486 up-regulated genes. These DEGs were mainly enriched in pathways related to the inflammatory responses. Subsequently, we identified 17 pyroptosis-related DEGs by comparing the 805 DEGs with the 223 pyroptosis-related genes. LASSO regression and RF algorithm analyses identified three CAVD diagnostic candidate genes (TREM1, TNFRSF11B, and PGF), which were significantly upregulated in the CAVD tissue samples. A diagnostic model was constructed with these 3 diagnostic candidate genes. The diagnostic model and the 3 diagnostic candidate genes showed good diagnostic performances with AUC values >0.75 in both the training and the validation cohorts based on the ROC curve analyses. CIBERSORT analyses demonstrated positive correlation between the proportion of M0 macrophages in the valve tissues and the expression levels of TREM1, TNFRSF11B, and PGF.ConclusionThree pyroptosis-related genes (TREM1, TNFRSF11B and PGF) were identified as diagnostic biomarkers for CAVD. These pyroptosis genes and the pro-inflammatory microenvironment in the calcified valve tissues are potential therapeutic targets for alleviating CAVD.

BackgroundGeriatric diseases (e.g., chronic diseases and geriatric syndromes) may result in impaired physical performance and a decline in the quality of life. The results of previous studies reported the positive effects of comprehensive community-based rehabilitation (CBR) services on physical and social functioning and psychosocial wellbeing. However, to provide adequate and personalised rehabilitation services, it is essential to understand the needs of the older adults population. There have been no studies on the need for CBR in older adults populations that consider their heterogeneity. Therefore, high-quality studies are required to recognise the heterogeneity and latent classes of CBR needs in older adults population groups. This study aims to identify the heterogeneity of the rehabilitation needs of older adults in the community and explore whether older adults with similar characteristics have similar needs through a cross-sectional survey and latent class analysis (LCA) to provide support for personalised rehabilitation services.MethodsThe study is structured into four phases. The first phase will focus on constructing a comprehensive questionnaire to assess rehabilitation needs. In the second phase, a pilot study will be conducted to evaluate the reliability and validity of the completed questionnaire. This step ensures the robustness of the instrument for data collection. The third phase will involve cross-sectional surveys using the finalised questionnaires to collect the necessary data from the targeted population. The fourth phase will focus on conducting LCA to determine the CBR needs of the older adult population.DiscussionThe results of this study will provide novel and critical information for a better understanding of the rehabilitation needs, potential categories, and influencing factors of older adults in the community. The study will be conducted in Guizhou Province in western China, where economic and social development is relatively low, and the results will inform and benefit other regions and developing countries facing similar challenges. However, because of the complete social security and rehabilitation service systems in developed areas, our research results may not fully reflect the situation in these areas. Future studies may need to be conducted in places with different levels of social development.Clinical trial registrationhttps://www.chictr.org.cn/showproj.html?proj=191398, ChiCTR2300071478.

In order to investigate the health risks of NO3− in rural drinking groundwater in Suihua, China and provide a basis for healthy drinking water, 40 sets of groundwater samples were collected in the Suihua area, and the average concentration of nitrate in the study area was 71.66 mg/L, statistical analysis software (SPSS19), Hydrogeochemical Analysis Software (AqQA) and groundwater pollution analysis software were used. Through water sample collection, chemical analysis and construction of human health risk model (HHRA), a qualitative and quantitative assessment of NO3− health risk was carried out for people of different ages and sexes, and it was concluded that there was NO3− pollution health risk in rural drinking groundwater in Suihua. Health risk level: infants > children > adult females > adult males. The evaluation provides a scientific basis for the prevention and control of NO3− pollution in groundwater and new ideas for preventing human health risks. HIGHLIGHTS The study identified the distribution pattern of nitrate in rural drinking groundwater in the Suihua area, providing a basis for preventing excessive use of fertilizers and pesticides.; The chemical characteristics of groundwater in the study area were reflected and described through the Durov plot.; A HHRA was first constructed in the Suihua area to evaluate the level of health risk in the study area.;

This study aimed to characterize the structure of Passiflora edulis Sims peel polysaccharide-zinc (WPEP-Zn) and its prebiotic effects. The structural characteristics of WPEP-Zn were determined using Fourier transform infrared spectrometer, X-ray diffractometer, scanning electron microscope, Congo red test, and thermogravimetric analyzer. The WPEP-Zn was used as carbon source to study its prebiotic effects on Lactobacillus plantarum, Lactobacillus delbrueckii (subsp.) bulgaricus, Lactobacillus brevis, and Streptococcus thermophilus. The results showed that WPEP-Zn had sugar and uronic acid content of 23.26% and 50.46%, respectively, whereas the zinc content was 10.64 mg/g. It had a triple helix structure compared with the Passiflora edulis Sims peel polysaccharide (WPEP). Its relative crystallinity decreased, the infrared absorption peak changed, the morphological structure altered, and the thermal stability enhanced. The optimal concentrations of WPEP-Zn used to promote the growth of the four bacteria strains were 3%, 2%, 2%, and 2%, respectively. The prebiotic effects of WPEP-Zn on L. plantarum, L. delbrueckii (subsp.) bulgaricus, and L. brevis were lower than WPEP, but its prebiotic effect on S. thermophilus was higher than the WPEP. The findings revealed the prebiotic effect of WPEP-Zn. The study on WPEP-Zn provided a basis for developing new polysaccharide-zinc supplements and the effective utilization of passion fruit resources.

The grouting technology of bed separations has been proved to be a new method which can meet the requirements of non-destructive mining and solid waste reduction. To effectively control the subsidence of ground structures caused by mining, the whole process of bed separation grouting mining is analyzed in a steady state based on the key stratum theory. For the first time, a “four zones” control model for surface subsidence under grouting bed separation was proposed, which includes natural zone, transition zone, warning zone, and protection zone, and the calculation formula for the “four zones” range was derived. Based on the engineering back-ground of controlling the subsidence of the ground coking plant at the 3501 panel, the proposed “four zones” model was validated by combining physical modelling and field measurement of subsidence. The results show that the surface subsidence curve of bed separation grouting in physical modelling shows an irregular “V” shape, and the surface subsidence of the panel first increases rapidly. After reaching the maximum subsidence, the surface subsidence first decreases rapidly, and then the reduction rate gradually slows down. The subsidence curve shows a clear “four zones” distribution, with a maximum subsidence of 1589 mm, appearing at the contact boundary between the natural zone and the transition zone. The subsidence of the contact boundary between the transition zone and the warning zone is 497.94 mm, and there is basically no subsidence within the protection zone. The predicted surface subsidence, horizontal deformation, slope, and curvature caused by mining under grouting conditions based on probability integral method are consistent with the field measured results, but significantly smaller than the predicted values under non-grouting conditions. It is determined that the surface deformation under grouting conditions meets the requirements of Grade I damage level for structures. Based on the practical engineering geology and observed data of the mine, the natural area is 261.19 m, the transition area is 246.09 m, the warning area is 655.25 m, and the protection area is 199.53 m. The proposed “four zones” control model provides a fundamental theoretical basis for studying the subsidence of bed separation grouting mining.

Mg-Cu alloys are promising antibacterial implant materials. However, their clinical applications have been impeded by their high initial biodegradation rate, which can be alleviated using nanotechnology by for example surface nanomodification to obtain a gradient nanostructured surface layer. The present work (i) produced a gradient nanostructured surface layer with a ∼500 µm thickness on a Mg-0.2 Cu alloy by a surface mechanical grinding treatment (SMGT), and (ii) studied the biodegradation behavior in Hank's solution. The initial biodegradation rate of the SMGTed samples was significantly lower than that of the unSMGTed original counterparts, which was attributed to the surface nanocrystallization, and the fragmentation and re-dissolution of Mg2Cu particles in the surface of the SMGTed Mg-0.2 Cu alloy. Furthermore, the SMGTed Mg-0.2 Cu alloy had good antibacterial efficacy. This work creatively used SMGT technology to produce a high-performance Mg alloy implant material.

Long non-coding RNAs (lncRNAs) play an important role in cancer metastasis. Exploring metastasis-associated lncRNAs and developing effective strategy for targeted regulation of lncRNA function in vivo are of utmost importance for the treatment of metastatic cancer, which however remains a big challenge. Herein, we identified a new functional lncRNA (denoted lncBCMA), which could stabilize the expression of eukaryotic translation elongation factor 1A1 (eEF1A1) via antagonizing its ubiquitination to promote triple-negative breast cancer (TNBC) growth and metastasis. Based on this regulatory mechanism, an endosomal pH-responsive nanoparticle (NP) platform was engineered for systemic lncBCMA siRNA (siBCMA) delivery. This NPs-mediated siBCMA delivery could effectively silence lncBCMA expression and promote eEF1A1 ubiquitination, thereby leading to a significant inhibition of TNBC tumor growth and metastasis. These findings show that lncBCMA could be used as a potential biomarker to predict the prognosis of TNBC patients and NPs-mediated lncBCMA silencing could be an effective strategy for metastatic TNBC treatment.

Abstract In order to deeply study the basic characteristics, diffusion laws, and flow laws of coal gangue and coal gangue slurry, the basic characteristic parameters of coal gangue and coal gangue slurry were obtained through particle size distribution test, electron microscope scanning test, X-ray diffraction test, X-ray fluorescence spectrum test, and angle of repose test. The conveying performance test of coal gangue slurry was carried out, and based on this, a simulation test of coal gangue slurry caving areas was designed. The diffusion and flow laws of coal gangue slurry under the same inclination angle were summarized, and the field test of fluidization filling in the caving areas was conducted. The results show that: (1) The water-to-gangue ratio was the main controlling factor for the conveying performance of coal gangue slurry. The extensibility, slump, and bleeding rate of the coal gangue slurry increased with the increase of the water-to-gangue ratio. (2) The diffusion profile of coal gangue slurry at different dip angles was arc-shaped, and the diffusion distance of slurry increased with the increase of infiltration time. However, there were differences in the sustained diffusion ability of different dip angles. (3) At the same time interval, the spatial accumulation patterns of scattered gangue in different regions will lead to differences in the diffusion speed of the slurry. (4) Both burying and hanging pipes in the falling area can safely and efficiently fill the gangue slurry. The diffusion distance of the caving areas in the test working face was basically consistent with the diffusion distance of the slurry in the simulation test of the coal gangue slurry caving areas.

Abstract Aims We sought to investigate the relationship between circulating tissue plasminogen activator (t‐PA) level and long‐term outcomes in stable coronary artery disease patients with or without aortic valve sclerosis (AVSc). Methods and results Serum levels of t‐PA were determined in 347 consecutive stable angina patients with (n = 183) or without (n = 164) AVSc. Outcomes were prospectively recorded as planned clinic evaluations every 6 months up to 7 years. The primary endpoint was a composite of cardiovascular death and rehospitalization due to heart failure. The secondary endpoint included all‐cause mortality, cardiovascular death, and rehospitalization due to heart failure. Serum t‐PA was significantly higher in AVSc than in non‐AVSc patients (2131.22 pg/mL vs. 1495.85 pg/mL, P 1840.68 pg/mL) were more likely to meet the primary and secondary endpoints (all P

To simulate the impact of the periodic wetting of the groundwater reservoir water level on the mechanical performance of the groundwater reservoir dam, we made a coal pillar-artificial dam assembly with a vertical interface, designed uniaxial compression tests with different number of wetting cycles, and considered two cycles as a gradient to explore the factors that affect assembly strength. We determined the strength model of coal pillar-artificial dam assembly in the groundwater reservoir after cyclic wetting and studied the mass variation induced by cyclic wetting. In addition, we analyzed the trends in the strength and deformation of the groundwater reservoir dam after cyclic wetting, proposed three failure modes, and clarified the internal mechanism for the cyclic wetting of the groundwater reservoir dam. The results demonstrated that with the increase in the number of wetting cycles, the internal water content, hydration reaction, and damage to the specimen were significantly enhanced and became more interrelated. The proposed cyclic wetting strength model of the groundwater reservoir dam can effectively represent the three-stage evolution law of the specimen strength curve. The compaction stage in the stress-strain curve of the cyclic wetting specimen is gradually extended, the straight line segment is shortened, the yield stage is also gradually lengthened, and the yield platform is obvious. The specimen failure patterns can be classified as unilateral columnar splitting, bilateral columnar splitting, and conical failure. The concrete will form ettringite and thaumasite with load-bearing capacity during the wetting process, and subsequently, C–S–H groups to invade the coal pillar.

Flocculation is important in the thickening process to improve the underflow concentration in thickeners for tailing suspensions. Traditional zone settling velocity (ZSV) functions ignore the effect of flocculant dosage on the ZSV and the thickening behavior of thickeners. To investigate the effect of flocculant dosage on the settling flux function, a series of batch settling tests were conducted at various flocculant dosages for unclassified and fine tailings. The correlation among flocculant dosage, solid fraction, and parameters in the ZSV function was revealed. Moreover, a simulation of continuous thickening based on the ZSV function was performed. Results indicated that flocculation influenced settling velocity and floc density. With an increased flocculant dosage, the settling velocity of floc increased, resulting in increased underflow concentration. Conversely, floc density decreased due to a stronger particle–particle interaction, leading to a decreased underflow concentration. HIGHLIGHTS Zhang's approach is validated for tailings at various initial solid fractions.; The correlation among flocculant dosage, solid fraction, and parameters in the ZSV function was revealed.; The coupling effect mechanism of flocculant dosage and solid fraction on thickening is proposed.;

Ti/Fe clad plate had attracted extensive attention because of its important application. In order to reduce the titanium layer thickness, the explosive welding of TA1 titanium foil to Q235 steel plate was carried out. The interfacial bonding performance was analyzed by micromorphology analysis and mechanical property test, and the formation process of interfacial wave and molten block in the vortex was simulated by smoothed particle hydrodynamics (SPH) method. The results showed that salt as pressure transfer layer used in explosive welding could play a good buffer effect on the collision between flyer and base layers. Regular waveforms were formed on the bonding interface, and the titanium foil/steel clad plate exhibited good welding quality and bonding property. The crest of the observed interfacial wave moved 200 μm from the beginning to the final formation, and it was important of jet on the formation of interfacial waveform. The interface was mainly bonded in the form of molten layer, and the grains near the interface were streamlined. Molten block containing intermetallic compounds and metal oxides appeared in the vortex of wave crest.

Abstract Given the deformation and instability of the layered floor of weakly cemented soft rock roadway, taking the mining roadway of Meihuajing Mine as the engineering background, the deformation and instability mechanism of the floor and the control measures of the surrounding rock under different stratum combination conditions are studied by using the methods of field investigation, numerical calculation and field test. The results show that: (1) the layered characteristics of surrounding rock in weakly cemented soft rock roadways are significant, and the combination structure of floor rock strata is complex and changeable. Under the influence of engineering disturbance, the roadway has a tendency of large deformation, especially floor heave. (2) The buckling instability of the layered floor is caused by the layered weakening effect, and the unstable zone, the substable zone and the stable zone are formed successively from the shallow to the deep. With the increase of the layer thickness, the integrity of the layered floor is improved, the layered weakening effect is reduced, and the overall stability of the layered floor is improved. (3) The existence of weak interlayer further degrades the integrity of layered floor, and the progressive failure of floor rock mass layer by layer is more significant under the influence of layered weakening effect. (4) The relatively hard rock stratum in the composite structure plays an important role in controlling the deformation and failure of the floor, playing the role of “local key stratum.” (5) Based on the cooperative control countermeasure of roof–rib–floor surrounding rock, the support technology of roof and ribs supported by blots mesh beam cable + floor supported by bolts grouting hardening + full section shotcrete is proposed. The results of numerical calculation and field test show that the support scheme greatly improves the stability of layered floor.

Effect of tempering time on microstructure and tensile properties of a low carbon martensitic stainless bearing steel was studied. Results showed that martensitic blocks became coarser and dislocation annihilation continuously took place during tempering process. It was also found that the number of M2C and M6C was increased firstly and then decreased, while their sizes were increased constantly. Moreover, the austenite content was increased due to the formation of reverted austenite. For tensile properties characterization, there was a transition from a ductile fracture to a quasi-cleavage fracture with increasing tempering time. Yield strength peaked when the steel was tempered for 24 h, followed by a constant decline. The abnormal decrease in elongation after long-term tempering was due to the formation of coarsening M23C6 at grain boundaries.

Ophibolin A, a fungal sesterterpene, exerts a pivotal influence in a diverse array of biological processes, encompassing herbicidal, bactericidal, fungicidal, and cytotoxic activities. Sixty genes associated with sesterterpene compound biosynthesis were obtained from Bipolaris eleusines via transcriptome sequencing, and those closely linked to ophiobolin A biosynthesis were subsequently filtered. A gene encoding 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) that catalyzes the first committed step of ophiobolin biosynthesis in the mevalonic acid (MVA) pathway was isolated and characterized using RACE (Rapid Amplification of cDNA Ends) technology from ophiobolin A-producing fungus, B. eleusines. The full-length cDNA of the B. eleusines HMGR gene (BeHMGR) was 3906 bp and contained a 3474 bp open reading frame (ORF) encoding 1157 amino acids. Sequence analysis revealed that deduced BeHMGR had high homology to the known HMGRs from Pyrenophora tritici-repentis and Leptosphaeria maculans. It had a calculated molecular mass of about 124.65 kDa and an isoelectric point (pI) of 6.90. It contained two putative HMG-CoA-binding motifs and two NADP(H)-binding motifs. Induced expression analysis of the BeHMGR gene by methyl jasmonate treatment using quantitative fluorescence PCR showed that it significantly elevated after 3 h of methyl jasmonate treatment, peaked at 6 h, and then gradually decreased. This demonstrates that BeHMGR gene expression is induced by methyl jasmonate.

Drought stress is a major meteorological threat to crop growth and yield. Barley (Hordeum vulgare L.) is a vital cereal crop with strong drought tolerance worldwide. However, the underlying growth properties and metabolomic regulatory module of drought tolerance remains less known. Here, we investigated the plant height, spike length, effective tiller, biomass, average spikelets, 1000-grain weight, number of seeds per plant, grain weight per plant, ash content, protein content, starch content, cellulose content, and metabolomic regulation mechanisms of drought stress in barley. Our results revealed that the growth properties were different between ZDM5430 and IL-12 under drought stress at different growth stages. We found that a total of 12,235 metabolites were identified in two barley genotype root samples with drought treatment. More than 50% of these metabolites showed significant differences between the ZDM5430 and IL-12 roots. The Kyoto Encyclopedia of Genes and Genomes pathway analysis identified 368 differential metabolites mainly involved in starch and sucrose metabolism, the pentose phosphate pathway, pyrimidine metabolism, phenylalanine, tyrosine, and tryptophan biosynthesis in ZDM5430 under drought stress, whereas the different metabolites of IL-12 under drought stress related to starch and sucrose metabolism, the pentose phosphate pathway, 2-oxocarboxylic acid metabolism, cutin, suberine and wax biosynthesis, carbon metabolism, fatty acid biosynthesis, and C5-branched dibasic acid metabolism. These metabolites have application in the tricarboxylic cycle, the urea cycle, the met salvage pathway, amino acid metabolism, unsaturated fatty acid biosynthesis, phenolic metabolism, and glycolysis. On the other hand, the expression patterns of 13 genes related to the abovementioned bioprocesses in different barley genotypes roots were proposed. These findings afford an overview for the understanding of barley roots’ metabolic changes in the drought defense mechanism by revealing the differently accumulated compounds.

Rainfall-induced landslides are a major hazard in the Three Gorges Reservoir area (TGRA) of China, encompassing 19 districts and counties with extensive coverage and significant spatial variation in terrain. This study introduces the Gradient Boosting Decision Tree (GBDT) model, implemented on the Google Earth Engine (GEE) cloud platform, to dynamically assess landslide risks within the TGRA. Utilizing the GBDT model for landslide susceptibility analysis, the results show high accuracy with a prediction precision of 86.2% and a recall rate of 95.7%. Furthermore, leveraging GEE’s powerful computational capabilities and real-time updated rainfall data, we dynamically mapped landslide hazards across the TGRA. The integration of the GBDT with GEE enabled near-real-time processing of remote sensing and meteorological radar data from the significant “8–31” 2014 rainstorm event, achieving dynamic and accurate hazard assessments. This study provides a scalable solution applicable globally to similar regions, making a significant contribution to the field of geohazard analysis by improving real-time landslide hazard assessment and mitigation strategies.

A chimeric protein, formed by two fragments of the conserved nucleocapsid (N) and S2 proteins from SARS-CoV-2, was obtained as a recombinant construct in Escherichia coli. The N fragment belongs to the C-terminal domain whereas the S2 fragment spans the fibre structure in the post-fusion conformation of the spike protein. The resultant protein, named S2NDH, was able to form spherical particles of 10 nm, which forms aggregates upon mixture with the CpG ODN-39M. Both preparations were recognized by positive COVID-19 human sera. The S2NDH + ODN-39M formulation administered by the intranasal route resulted highly immunogenic in Balb/c mice. It induced cross-reactive anti-N humoral immunity in both sera and bronchoalveolar fluids, under a Th1 pattern. The cell-mediated immunity (CMI) was also broad, with positive response even against the N protein of SARS-CoV-1. However, neither neutralizing antibodies (NAb) nor CMI against the S2 region were obtained. As alternative, the RBD protein was included in the formulation as inducer of NAb. Upon evaluation in mice by the intranasal route, a clear adjuvant effect was detected for the S2NDH + ODN-39M preparation over RBD. High levels of NAb were induced against SARS-CoV-2 and SARS-CoV-1. The bivalent formulation S2NDH + ODN-39M + RBD, administered by the intranasal route, constitutes an attractive proposal as booster vaccine of sarbecovirus scope.

This paper presents a study to evoke rat cortical functional activities, including hemodynamic and neural tissue signal changes, by monopulse electrical stimulation with a microelectrode array using functional optical coherence tomography (fOCT). Based on the principal component analysis and fuzzy clustering method (PCA-FCM), the hemodynamic response of different size blood vessels in rat cortex are analyzed, showing that the hemodynamic response of the superficial large blood vessels is more concentrated. In the regions of neural tissue where blood vessels are removed, positive significant pixels (the intensity of the pixel for five consecutive frames is greater than the average value plus triple standard deviation) and negative significant pixels (the intensity of the pixel for five consecutive frames is less than the average value minus triple standard deviation) exist, and the averaged intensity signal responds rapidly with an onset time of ~20.8 ms. Furthermore, the hemodynamic response was delayed by ~3.5 s from the neural tissue response. fOCT can provide a label-free, large-scale and depth-resolved map of cortical neurovascular activation, which is a promising technology to monitor cortical small-scale neurovascular activities.

This study focused on the stability control of roof in long wall stratified mining of large dip angle thick coal seam. Taking Panbei Coal Mine in Huainan as an example, the effect mechanism of water content, compression ratio and grain size grading on the compression characteristics of gangue is analyzed by comprehensive application of gangue lateral compression test, 3D imaging borehole detection test, physical simulation and numerical simulation method. In addition, the effect of these factors on the stability of the reclaimed roof were also analyzed, the structural characteristics and stress state of the reclaimed roof were obtained, the dynamic behavior of the reclaimed roof was revealed, the stability control measures of the reclaimed roof were formulated, and the control effect of the reclaimed roof stability was evaluated. The research results indicate that the clay minerals of goaf mudstone and sandy mudstone are beneficial for the secondary cementation of gangue. The contact state and stress state of the gangue during compression under load are constantly adjusted, manifested as the compression and bonding degree of the lower and upper gangue being higher than that of the middle, with the compression and bonding degree of the middle and upper gangue being the smallest, and the regenerated roof being more prone to damage. The particle size distribution of gangue is the main factor affecting the shear strength of recycled roof. As the particle size of the gangue increases, the regenerated roof exhibits ductile failure that slides along the shear line to the staggered and bulging of the gangue particles. That is, the degree of mining fracture in the lower part of the regenerated roof tends to be milder than in the middle and upper parts, and the middle and upper parts are the key prevention and control areas for the stability control of the regenerated roof. The deflection of the main stress can lead to the fracture of the regenerated roof, so a plan for the collapse of the regenerated roof and the grouting.

Abstract Aims Lactate/albumin ratio (L/A) is a recognized prognostic index of patients with heart failure (HF) after myocardial infarction (MI). We aim to evaluate the prognostic value of L/A ratio in predicting in‐hospital mortality for those patients. Methods and results We enrolled qualified patients from Medical Information Mart for Intensive Care IV database for retrospective study. A receiver operating characteristic (ROC) curve of the subjects was applied to determine the predicted value and the best cut‐off value of L/A on admission. Univariate/multivariate Cox regression analysis and restricted cubic splines (RCS) were performed to identify the association between hospital admission and hospital mortality. The Kaplan–Meier (KM) method was used to draw the survival curve of the two groups with different L/A levels at admission. L/A values at admission were significantly higher in the death groups than the survival groups [1.36 (1.20) vs. 0.62 (0.36), P

Abstract Aims Inflammatory biomarkers, including CRP, the neutrophil‐to‐lymphocyte ratio (NLR), and the neutrophil‐to‐eosinophil ratio (NER), may predict outcomes in cancer. However, their value in immune checkpoint inhibitor (ICI) therapy‐associated cardiotoxicity remains elusive. We aimed to characterize the relationship of inflammatory markers with severity of ICI‐related cardiotoxicities (iRCs) and prognosis among patients with iRCs. Methods Patients who were diagnosed with iRCs between January 2019 and December 2021 were retrospectively enrolled and were dichotomized based on iRC severity into low‐grade (grade 1–2) vs. high‐grade (grade 3–4) groups. Results Forty‐seven patients were included. The median time‐to‐event from first ICI infusion to onset of iRCs was 35 days (IQR: 19.0–65.5 days). When compared with respective baseline values, cardiac biomarkers and inflammatory markers were significantly elevated at onset of iRCs. Compared with low‐grade iRCs, NER at iRC onset was significantly increased among patients with high‐grade iRCs (Group × Time, P

With an increasing mining depth and intensity in Chinese coal mines, unprecedented frequency and intensity of mining tremors are attracting extensive attention from the government and society. Under the initiatives from the government and the intensive investigations, significant progress has been made in the research and engineering practice, which greatly enhances the understanding and hazard control of the mining tremors. This paper reviewed the state-of-the-art occurrence mechanism, damage characteristics and control of the mining tremors in Chinese coal mines and demonstrated their main challenges. The conclusions are as follows: due to the complicated occurrence conditions of mining tremors, various definitions and classifications of mining tremors have been proposed from different aspects. This paper has summarized these definitions and classifications and defines mining tremors from special and general aspects, respectively, which classify mining tremors in terms of phenomena and occurrence mechanisms. Because mining tremors are widespread in all mining countries in the world, targeted mining tremor hazards prevention and control are urgently needed. Although the research started late in China, the hazard control of mining tremors and the emergency dealing capacity have been significantly enhanced in recent years due to the implementation of the regulations and rules from the government for mining tremors prevention and control. It has been summarized that from the macro view, the triggering of mining tremors is mainly induced by the coal pillar failure, roof breakage and movement and fault slip, and from the micro view, the triggering of mining tremors mainly includes tension rupture, implosion rupture and shear rupture. The mining-tremor-induced hazards have been surveyed in terms of source parameters, seismic wave attenuation and seismic wave disturbances, and the methods for evaluating surface and underground damage triggered by mining tremors have been also concluded. To achieve hazard source control and precise prevention, the current situation of mining tremors treatment in Chinese coal mines has been outlined. At present, Chinese coal mines, it is still far away to achieve accurate hazard source identification and full hazard control. Therefore, to support the national strategy of deep resources development and energy security, future studies should focus on investigating the hazard-forming mechanism, damage effect and prevention and control technologies of mining tremors.

Abstract Background This retrospective study aimed to compare the outcomes of toric implantable collamer lens (TICL) surgery with those of implantable collamer lens (ICL) implantation combined with limbal relaxing incision (LRI) in patients with low myopia and astigmatism. Methods A total of 40 eyes of 28 patients who underwent TICL implantation and 40 eyes of 27 patients who underwent ICL implantation combined with manually LRI between 2021 and 2022 were included. Primary outcomes were manifest sphere and cylinder, intraocular pressure, visual acuity, and astigmatism parameters at 1 day, 1 week, and 1, 3, and 6 months postoperatively. Results The two surgeries showed comparable effects on manifest sphere and cylinder, intraocular pressure, and visual acuity (all p > 0.1). Surgery-induced astigmatism (SIA) was maintained as stable in the TICL group (1.73 to 1.68, p = 0.420), but was significantly reduced in the ICL/LRI group (1.74 to 1.17, p = 0.001) from preoperative to postoperative 6 months. The TICL group displayed significantly higher SIA and correction index at postoperative 1, 3, and 6 months than the ICL/LRI group (at 6 months: SIA, 1.68 (1.26, 1.96) vs., 1.17 (1.00, 1.64), p = 0.010; CI: 0.98 (0.78, 1.25) vs. 0.80 (0.61, 1.04), p = 0.018). No complications occurred during follow-up. Conclusions The effects of ICL/LRI are comparable to those of TICL in correcting myopia. TICL implantation displays better astigmatism correction than ICL/LRI.

The landfalls of the tropical cyclone (TC) along the coast of China have caused huge economic damages. There are approximately nine TC landfalls in China every year. It will be beneficial if the landfall frequency can be predicted in advance. Inspired by the study of Sparks and Toumi (Commun Earth Environ, 30-1-2020), six datasets, including four ocean reanalyses and two object analyses from 1993 to 2019, are employed to study the consistency in the relationship between the thermocline temperature anomalies at different longitudes and the frequency of TC landfalls along the coastal areas of China (South China, East China, and the whole of China). The thermocline temperature anomalies at different longitudes are tested in order to confirm our hypothesis that the eastward and westward transports of ocean heat from the warm pool are the causes of the significant correlations. The results show some significant correlations at various longitudes, and the temperature anomalies can predict the TC landfall frequency for several months or longer. Further study also shows the close relationship between the ocean heat transport and the sea surface temperature anomalies at the genesis locations of TC landfalls. The locations of the western Pacific subtropical high (WPSH) during high-frequency TC landfall years also show favorable spatial patterns to the TC landfall in South China and East China, respectively. In years with a high TC frequency in South China, the westward displacement of the WPSH ridge steers TC toward South China, while during high-frequency TC landfall years in East China, WPSH is located further north, and the westward extension of the ridge is in close proximity to the East China Sea.

This study aimed to evaluate the efficacy of amylase in hydrolyzing complex carbohydrates of different parts of Ganoderma spp. The aqueous extracts of the Ganoderma samples were analyzed for their selected nutritional composition and physicochemical properties. The purified extracts were also structurally characterized. The aqueous canopy extracts of red–purple Ganoderma had a notably higher total sugar and saponin content than their stalks, but not for the black-type Ganoderma. The enzymatic extraction effectively improved the extraction yields, whereas the amounts of sugars and saponins in some extracts were increased after the enzymatic treatment. The results also showed that only those enzyme-treated cultivated black Ganoderma canopy had increased total sugar and total saponin content. The antioxidant activities of all stalk extracts were higher than the canopy extracts. Their emulsifying properties were comparable with lecithin due to their high saponin content. Therefore, these extracts are new natural emulsifiers.

ObjectivePatients with temporal lobe epilepsy (TLE) often exhibit attention function impairment. The orienting network is the subsystem of the attention network that has not been fully studied. In this study, we used eye-tracking technology with an attention network test (ANT)-based task to assess the orienting function of TLE patients, aiming to characterize their eye movement patterns.MethodsA total of 37 TLE patients and 29 healthy controls (HCs) completed the ANT task based on eye-tracking technology. Orienting function damage was mainly assessed by the ANT orienting effect. Eye movement metrics, such as mean first goal-directed saccade latency (MGSL), total saccades, and saccade amplitudes, were compared between groupsResultsThe TLE patients had a significantly lower ANT orienting effect (HC, 54.05 ± 34.05; TLE, 32.29 ± 39.54) and lower eye-tracking orienting effect (HC, 116.98 ± 56.59; TLE, 86.72 ± 59.10) than those of the HCs. The larger orienting effects indicate that orienting responses are faster when receiving a spatial cue compared with a center cue. In the spatial cue condition, compared with HCs, the TLE group showed a longer first goal-directed saccade latency (HC, 76.77 ± 58.87 ms; TLE, 115.14 ± 59.15 ms), more total saccades (HC, 28.46 ± 12.30; TLE, 36.69 ± 15.13), and larger saccade amplitudes (HC, 0.75° ± 0.60°; TLE, 1.36° ± 0.89°). Furthermore, there was a positive correlation of the orienting-effect score between the ANT task and eye-tracking metrics (r = 0.58, p < 0.05).ConclusionWe innovatively developed a new detection method using eye-tracking technology in combination with an ANT-based task to detect the orienting function in TLE patients. The current research demonstrated that TLE patients had a significant orienting dysfunction with a specific saccade pattern characterized by a longer first goal-directed saccade latency, more total saccades, and larger saccade amplitudes. These oculomotor metrics are likely to be a better indicator of orienting function and may potentially be used for behavioral-based interventions and long-term cognition monitoring in TLE patients.

AbstractBased on the true triaxial dynamic anisotropic permeability model of coal and rock, a multi field coupling mathematical model of structurally anisotropic coal seams under true triaxial stress was established, and a numerical simulation experiment of porous dry gas drainage in anisotropic coal seams was conducted using the COMSOL Multiphysics numerical simulation software. It was found that with an increase in the permeability anisotropy ratio, the greater the ratio of the long and short axes of the elliptical pressure isoline was, the denser was the gas streamline in the dominant seepage direction. As the permeability anisotropy ratio increased, the gas extraction volume and rate decreased. When the permeability was isotropic, the gas drainage volume of the uniformly arranged rectangular boreholes was the largest. However, when the permeability was anisotropic, the gas extraction volume and rate were the largest along the y-direction layout (perpendicular to the dominant seepage direction), followed by the rhombus layout, and the smallest along the x-direction layout. Based on the test results, an optimal arrangement principle of multi holes pumping boreholes along the vertical dominant seepage direction was proposed.

The share of new energy in China's energy consumption structure is expanding, posing serious challenges to the national grid's stability and reliability.As a result, it is critical to construct large-scale reliable energy storage infrastructure and smart microgrids. Based on the spatial resource endowment of abandoned mines' upper and lower wells and the principle characteristics of the gravity energy storage system, an intelligent microgrid system model for abandoned mines based on gravity energy storage is proposed, and the system's feasibility and key influencing factors are discussed and analyzed from the standpoint of economic benefits. The gravity energy storage system principle, system structure, subsurface powerhouse, underground storage, and transit system are all examined and analyzed.The viability of establishing intelligent microgrid systems in abandoned mines is proved using the resource conditions, technical conditions, economic advantages, and social benefits of Panyidong Mine in Huainan Coal Mine.The findings indicate that the project concept has good economic and social benefits as well as practical viability.Next, from the perspectives of technology, policy, and the ecological environment, several recommendations for the development of a smart microgrid system based on gravity energy storage power station are made. This study presents a novel concept for the advancement of energy storage technology and the reuse of abandoned mine resources, which is critical to the long-term development of abandoned mine resources and the advancement of energy storage technology.

Objective: Myocardial ischemia/reperfusion (MI/R) injury is a major cause of cardiac tissue damage, with high disability and death rates. Although both dexmedetomidine (Dex) and propofol (PPF) have been indicated to alleviate MI/R injury in rat models, the effects of the combined use of these two drugs remain unclear. This study aimed to investigate the combined effects of Dex and PPF against MI/R injury and related mechanisms. Methods: A rat model of MI/R injury was established and used to explore the combined effects of Dex and PPF on MI/R injury. Hematoxylin-eosin (HE) and Masson staining were used for histopathological evaluation. 2,3,5-triphenyltetrazolium chloride (TTC), echocardiography, terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining were used to determine myocardial infarction size, cardiac function, and apoptosis, respectively. Enzyme-linked immunosorbent assay (ELISA) was performed to assess myocardial function and oxidative stress (OS). Autophagy was observed through transmission electron microscopy. Moreover, western blotting was conducted to detect autophagy markers and the AMPK pathway. Results: The combination of Dex and PPF alleviated histopathological injury, reduced myocardial infarction, and rescued cardiac dysfunction in MI/R rats. Furthermore, Dex combined with PPF decreased the levels of MDA and ROS and increased the SOD level in MI/R rats. Besides, Dex combined with PPF inhibited myocardial apoptosis in MI/R rats. After combined treatment with Dex and PPF, the number of autophagosomes, expression levels of Beclin-1 and LC3II/LC3I were elevated, while the expression levels of p62 were reduced in MI/R rats. The combined use of Dex and PPF activated the AMPK pathway in MI/R rats. Compound C (an AMPK inhibitor) could abolish the combined effects of Dex and PPF on alleviating myocardial injury and enhancing autophagy in MI/R rats. Conclusion: The combination of Dex and PPF attenuated MI/R injury in rats, which may be associated with the activation of the AMPK signaling pathway.

Bacterial infectious diseases are one of the leading causes of death worldwide. Even with the use of multiple antibiotic treatment strategies, 4.95 million people died from drug-resistant bacterial infections in 2019. By 2050, the number of deaths will reach 10 million annually. The increasing mortality may be partly due to bacterial heterogeneity in the infection microenvironment, such as drug-resistant bacteria, biofilms, persister cells, intracellular bacteria, and small colony variants. In addition, the complexity of the immune microenvironment at different stages of infection makes biomaterials with direct antimicrobial activity unsatisfactory for the long-term treatment of chronic bacterial infections. The increasing mortality may be partly attributed to the biomaterials failing to modulate the active antimicrobial action of immune cells. Therefore, there is an urgent need for effective alternatives to treat bacterial infections. Accordingly, the development of immunomodulatory antimicrobial biomaterials has recently received considerable interest; however, a comprehensive review of their research progress is lacking. In this review, we focus mainly on the research progress and future perspectives of immunomodulatory antimicrobial biomaterials used at different stages of infection. First, we describe the characteristics of the immune microenvironment in the acute and chronic phases of bacterial infections. Then, we highlight the immunomodulatory strategies for antimicrobial biomaterials at different stages of infection and their corresponding advantages and disadvantages. Moreover, we discuss biomaterial-mediated bacterial vaccines’ potential applications and challenges for activating innate and adaptive immune memory. This review will serve as a reference for future studies to develop next-generation immunomodulatory biomaterials and accelerate their translation into clinical practice. Public summary: • Insights into the intricate facets of the immune microenvironment hold the key to pioneering clinical strategies in combatting bacterial infections. • The design principles for antimicrobial biomaterials vary depending on the immune microenvironment at different stages of infection. • Immunomodulatory biomaterials display robust antimicrobial efficacy and vaccine attributes in animals and clinical trials, promising for intractable infections.

BackgroundSyphilis has caused epidemics for hundreds of years, and the global syphilis situation remains serious. The reported incidence rate of syphilis in Zhejiang Province has ranked first in the province in terms of notifiable infectious diseases for many years and is the highest in China. This study attempts to use the scaling law theory to study the relationship between population size and different types of syphilis epidemics, while also exploring the main driving factors affecting the incidence of syphilis in different regions.MethodsData on syphilis cases and affected populations at the county level were obtained from the China Disease Control and Prevention Information System. The scaling relationship between different stages of syphilis and population size was explained by scaling law. The trend of the incidence from 2016 to 2022 was tested by the joinpoint regression. The index of distance between indices of simulation and observation (DISO) was applied to evaluate the overall performance of joinpoint regression model. Furthermore, a multivariate time series model was employed to identify the main driving components that affected the occurrence of syphilis at the county level. The p value less than 0.05 or confidence interval (CI) does not include 0 represented statistical significance for all the tests.ResultsFrom 2016 to 2022, a total of 204,719 cases of syphilis were reported in Zhejiang Province, including 2 deaths, all of which were congenital syphilis. Latent syphilis accounted for 79.47% of total syphilis cases. The annual percent change (APCs) of all types of syphilis, including primary syphilis, secondary syphilis, tertiary syphilis, congenital syphilis and latent syphilis, were − 21.70% (p

Severe bronchopulmonary dysplasia (BPD) is a chronic lung disorder that primarily affects premature babies with extremely low birth weight and involves in multiple organ system; no effective pharmacotherapy for this disease exists, and mortality remains high. Based on the evidence from previous preclinical studies and phase I clinical trials, this study aims to test the safety of intravenous application of a single dose of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) in patients with severe BPD. The Mesenchymal Stem cells for Bronchopulmonary Dysplasia Treatment (MSBDT) trial is a single center, open-label, dose-escalation phase I clinical trial. Severe BPD patients were enrolled in Children Hospital of Chongqing Medical University, Chongqing, China. The first six patients were treated with low-dose hUC-MSCs (1 × 106 cells/kg) and the next seven patients were treated with high-dose hUC-MSCs (5 × 106 cells/kg). This study is registered with ClinicalTrials.gov, number NCT03558334. No prespecified infusion-associated adverse events, immediate complication, respiratory or cardiovascular compromise were observed during infusion and 24 h after infusion. No significant changes in safety laboratory values were observed. One death event occurred in the low-dose group on study day 10, and one death event occurred in the high-dose group on study day 24, while, after review in detail, the two cases are not believed to be infusion-associated events. In conclusion, intravenous application of a single dose of hUC-MSCs was tolerated in thirteen patients with severe BPD.