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Coating conducting polymers onto active cathode materials has been proven to mitigate issues at high current densities stemming from the limited conducting abilities of the metal-oxides. In the present study, a carbon coating was applied onto nickel-rich NMC622 via polymerisation of furfuryl alcohol, followed by calcination, for the first time. The formation of a uniform amorphous carbon layer was observed with scanning- and transmission-electron microscopy (SEM and TEM) and X-ray photoelectron spectroscopy (XPS). The stability of the coated active material was confirmed and the electrochemical behaviour as well as the cycling stability was evaluated. The impact of the heat treatment on the electrochemical performance was studied systematically and was shown to improve cycling and high current performance alike. In-depth investigations of polymer coated samples show that the improved performance can be correlated with the calcination temperatures. In particular, a heat treatment at 400 °C leads to enhanced reversibility and capacity retention even after 400 cycles. At 10C, the discharge capacity for carbon coated NMC increases by nearly 50% compared to uncoated samples. This study clearly shows for the first time the synergetic effects of a furfuryl polymer coating and subsequent calcination leading to improved electrochemical performance of nickel-rich NMC622.

ARPC1B is a component of the actin-related protein 2/3 complex (Arp2/3), which is required for actin filament branching. Kahret al. show that ARPC1B deficiency in humans is associated with severe multisystem disease that includes platelet abnormalities, eosinophilia, eczema and other indicators of immune disease.

Platelets are critical to hemostasis and thrombosis. Upon detecting injury, platelets show a range of responses including the release of protein cargo from α-granules. This cargo is synthesized by platelet precursor megakaryocytes or endocytosed by megakaryocytes and/or platelets. Insights into α-granule biogenesis have come from studies of hereditary conditions where these granules are immature, deficient or absent. Studies of Arthrogryposis, Renal dysfunction, and Cholestasis (ARC) syndrome identified the first proteins essential to α-granule biogenesis: VPS33B and VPS16B. VPS33B and VPS16B form a complex, and in the absence of either, platelets lack α-granules and the granule-specific membrane protein P-selectin. Gray Platelet Syndrome (GPS) platelets also lack conventionally recognizable α-granules, although P-selectin containing structures are present. GPS arises from mutations affecting NBEAL2. The GPS phenotype is more benign than ARC syndrome, but it can cause life-threatening bleeding, progressive thrombocytopenia, and myelofibrosis. We review the essential roles of VPS33B, VPS16B, and NBEAL2 in α-granule development. We also examine the existing data on their mechanisms of action, where many details remain poorly understood. VPS33B and VPS16B are ubiquitously expressed and ARC syndrome is a multisystem disorder that causes lethality early in life. Thus, VPS33B and VPS16B are clearly involved in other processes besides α-granule biogenesis. Studies of their involvement in vesicular trafficking and protein interactions are reviewed to gain insights into their roles in α-granule formation. NBEAL2 mutations primarily affect megakaryocytes and platelets, and while little is known about NBEAL2 function some insights can be gained from studies of related proteins, such as LYST.

Atypical hemolytic uremic syndrome is a thrombotic microangiopathy, which is linked to hereditary or autoimmune defects in complement activators or regulators present in blood and on vascular endothelial cells. Acute thrombotic microangiopathy episodes are typically preceded by infections, which by themselves would not be expected to manifest HUS. Thus, it is possible that the host immune response contributes to the precipitation of aHUS. However, the mechanisms involved are not fully understood. We hypothesized that neutrophils trigger aHUS via initiating platelet aggregate formation on complement-activated endothelial cells. Methods: We investigated neutrophil adhesion to complement-activated endothelial cells under static and flow conditions in vitro and ex vivo. Results: Our results show that complement activation on endothelial cells promotes neutrophil adhesion, which is significantly reduced when the complement terminal pathway is blocked. When neutrophils and platelets are perfused simultaneously, neutrophils adhering to endothelial cells also induce the formation of platelet-neutrophil aggregates on these cells. Sera from patients with aHUS recapitulated these results. Discussion: Therefore, our findings of (i) neutrophils adhering to complement-activated endothelial cells, (ii) the formation of neutrophil-platelet aggregates on endothelial cells, and (iii) the ability of aHUS serum to induce similar effects identify a possible role for neutrophils in aHUS manifestation.

In der vorliegenden Evaluationsstudie wurden Studierende und Lehrende einer österreichischen Universität befragt, in welchen Bereichen der Lehre sich Studierende eine stärkere Flexibilisierung wünschen und inwieweit sich diese Wünsche von der Einschätzung Lehrender unterscheiden. Die Ergebnisse zeigen, dass Studierende und Lehrende in ihren Präferenzen zur Lehrveranstaltungsgestaltung zum großen Teil übereinstimmen, sich aber auch in einzelnen Bereichen unterscheiden. Insbesondere hinsichtlich einzelner Aspekte von Zeit, Inhalt, Lernressourcen und Support, Beurteilung und Bereitstellung schätzen Studierende Flexibilität bedeutsamer ein als Lehrende. Es zeigen sich hier bedeutsame statistische Effekte.

Most research universities have concrete policies for navigating the conflicts of interest of faculty members. Policies that might constrain university administrators acting on behalf of their schools, so-called institutional conflicts of interest, are absent or poorly developed at most places that could benefit from them. Researchers have argued for the illustration of institutional conflicts as a foundation for policy development. Here, we show the failure of research accountability when a faculty member made massive gifts to a leading American public research university, the University of Washington in Seattle, thereby creating allegiances that undermined commitments to academic values. Correspondence, some from thousands of pages acquired through the Washington State Public Records Act, show faculty colleagues, department chairs, deans, a provost, presidents, and the Board of Regents soliciting and accepting the donor's money but not sufficiently guarding the integrity of science when that was required. These records offer a rare look inside a university scientific misconduct investigation, a process typically shrouded in secrecy under the guise of confidentiality. They amount to a forensic analysis of what can go wrong with science at the nexus of a secret history of misconduct, spectacularly ambitious science, and large donations. The inabilities of federal and state authorities to reckon with institutional conflicts of interest are highlighted. The collective inaction can be understood within Lessig’s framework of institutional corruption. The failings described herein are metaphorical holes in the safety net intended to protect the integrity of American science, a shared practice that is under increasing strain. All public records are available from the authors upon request. Those cited here are included in an appendix posted by the journal.

In contrast to most common gene delivery techniques, lentiviral vectors allow targeting of almost any mammalian cell type, even non-dividing cells, and they stably integrate in the genome. Therefore, these vectors are a very powerful tool for biomedical research. Here we report the generation of a versatile new set of 22 lentiviral vectors with broad applicability in multiple research areas. In contrast to previous systems, our platform provides a choice between constitutive and/or conditional expression and six different C-terminal fusions. Furthermore, two compatible selection markers enable the easy derivation of stable cell lines co-expressing differently tagged transgenes in a constitutive or inducible manner. We show that all of the vector features are functional and that they contribute to transgene overexpression in proof-of-principle experiments.

In der Praxis wird zumeist angenommen, dass die Ergebnisse in einem Aufnahmeverfahren individuelle Unterschiede in den zu erfassenden Fähigkeiten fair messen. Der vorliegende Beitrag gibt einen Überblick über Modelle zur Fairness von Aufnahmeverfahren und deren Überprüfung in der Praxis anhand von Beispielen zum Aufnahmeverfahren für Medizinische Studiengänge in Österreich (MedAT). Der Beitrag arbeitet zudem auch den engen Zusammenhang zwischen Fairness und anderen Gütekriterien heraus und veranschaulicht die Vorteile einer stark theoriegeleiteten automatischen Itemgenerierung (AIG), um auf die erhöhten Qualitätsanforderungen bei modernen Aufnahmeverfahren reagieren zu können.

Arrhythmogenic cardiomyopathy (AC) is a rare mostly hereditary disease, in which fibro-fatty tissue replaces cardiomyocytes. Typically, the first alterations of the disease can be encountered in the epicardium of the right ventricle in adolescent patients. From there, the disease usually progresses over time. Besides the development of heart failure, the clinical significance of the disease is determined by the predisposition to potentially lethal ventricular arrhythmias. Hence, a majority of patients with AC require an implantable cardioverter-defibrillator (ICD) to be protected from sudden cardiac death. A recently developed alternative to transvenous systems are subcutaneous ICDs (S-ICD), associated with a lower risk of device-related complications such as endocarditis since no foreign material is implanted within the heart and vascular system. In this report, we describe and discuss our experience with the implantation of a S-ICD in a patient with AC, who had low QRS voltage and persistent atrial fibrillation precluding successful S-ICD implantation, as well as the challenges encountered during subsequent transvenous lead implantation.

Background: The application of a social theory of learning and the notion of situated learning as a theoretical basis for understanding students' learning in PE is broadly recognised. Nevertheless, it is far more unusual for this theoretical approach to provide a basis for understanding learning processes in talent development in elite sport. Purpose: The purpose is to explore talent development and skill learning in youth elite football as a situated activity in a highly specialised community of practice (CoP) shared by talented male under-18 football players. From a social theory of learning perspective we seek to identify how the football player's ability to stay in elite football probably is influenced by the construction of the social practices in a football community of practice. Participants and setting: The research took the form of a case study of the best under-18 team from two Danish football clubs. Both teams competed in the best Danish tournament series and trained 16-20 hours a week. Data collection: Data were collected from participant observation and formal as well as informal interviews with four selected football players. The participant observations took place every day for three weeks at each club, and were performed as a "moderate participant", who observed day-to-day training, acted as an assistant coach, acted as a player in some exercises, and observed matches and other activities. Data analysis: The analysis of data was inspired by an ethnographical approach that involves a step-wise shift from data collection, analysis and description to write-up and theory, and an interpretive naturalistic approach. Findings: On the basis of observed differences in the organisation of the players' mutual relations, we established a categorisation of the two communities of practice into (1) a Small group and Position-specific based Community of Practice (SgPCoP), and (2) a Team based Community of Practice (TbCoP). The organisation influenced the ways in which players participated in the CoP, i.e. how their learning trajectories evolve in the community. The SgPCoP, rather than the TbCoP, created opportunities for players to "play upwards", mirror older players, and the feeling of being recognised by the coach. Conclusions: Due to the organisation of the training sessions, peripheral players in the SgPCoP experienced inbound learning trajectories, i.e. training-inclusion and multimembership, and assumed ownership of their long-term development as football players. This was only the case to a lesser extent in the TbCoP. (Contains 1 note.)

Ultrasound exams need skilled examiners, and simulation-based training could provide standardized and safe skill training. This study aims to give an overview of different types of virtual-reality simulators for use in abdominal diagnostic ultrasound training in radiology. Fifteen specialized radiologists and radiological residents were presented with two similar cases on four different simulators for abdominal ultrasound training. A feedback sheet for each individual simulator and for an overall comparison was filled out by all participants. All means of scores were compared, and simulators were ranked from least to most favorable. One simulator was ranked most favorable in seven out of nine questions, but none of the mean scores had statistically significant differences. All simulators were recommended for training radiologists, and all simulators were perceived to benefit trainees more than experienced ultra-sonographers.

Accurate knowledge of the spatial magnetic field distribution is necessary when measuring field gradients. Therefore, a MEMS magnetic field gradiometer is reported, consisting of two identical, but independent laterally oscillating masses on a single chip. The sensor is actuated by Lorentz force and read out by modulation of the light flux passing through stationary and moving arrays of the chip. This optical readout decouples the transducer from the electronic components. Both phase and intensity are recorded which reveals information about the uniformity of the magnetic field. The magnetic flux density is measured simultaneously at two points in space and the field gradient is evaluated locally. The sensor was characterised at ambient pressure by performing frequency and magnitude response measurements with coil and various different permanent magnet arrangements, resulting in a responsivity of 35.67 V/T and detection limit of 3.07 µT/ Hz (@ 83 Hz ENBW). The sensor is compact, offers a large dynamic measurement range and can be of low-cost by using conventional MEMS batch fabrication technology.

Today's young semi-professional football players are expected to continue their education while honing their talents as footballers. This means they must balance the contradictory demands that come from their education establishments and their football clubs. The present study explores how young Danish male football talents experience and describe these different forces in their life space (Lewin, 1951). Data for the study are gathered using a narrative and qualitative approach, including four focus group interviews with 25 footballers aged 15-19, followed by individual qualitative interviews with eight of the footballers. The elite football culture has an almost magnetic attraction for the young footballers in the study. Even if the espoused value (Schein, 1990) of a good set of academic qualifications does not entirely measure up to this, the insistence from the outside world on the importance of completing one's education is manifest and associated with significant personal concerns, lower examinations results, stress, drop-out and mental breakdown. (Contains 1 figure.)

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Neutrophils deposit antimicrobial proteins such as myeloperoxidase and proteases on chromatin, which they release as neutrophil extracellular traps (NETs). Neutrophils also carry key components of the complement alternative pathway (AP) such as properdin or complement factor P (CFP), complement factor B (CFB) and C3. However, the contribution of these complement components and complement activation during NET formation in the presence and absence of bacteria is poorly understood. We studied complement activation on NETs and a Gram-negative opportunistic bacterial pathogen Pseudomonas aeruginosa (PA01, PAKwt, PAKgfp). Here we show that anaphylatoxin C5a, formyl-Methionyl-Leucyl-Phenylalanine (fMLP), and phorbol myristate acetate (PMA) that activates NADPH oxidase induce the release of CFP, CFB and C3 from neutrophils. In response to PMA or P. aeruginosa, neutrophils secrete CFP, deposit it on NETs and bacteria, and induce the formation of terminal complement complexes (C5b-9). A blocking anti-CFP antibody inhibited AP-mediated but not non-AP-mediated complement activation on NETs and P. aeruginosa. Therefore, NET-mediated complement activation occurs via both AP- and non AP-based mechanisms, and AP-mediated complement activation during NETosis is dependent on CFP. These findings suggest that neutrophils could use their AP tool kit to readily activate complement on NETs and Gram-negative bacteria such as P. aeruginosa, whereas additional components present in the serum help to fix non-AP-mediated complement both on NETs and bacteria. This unique mechanism may play important roles in host defense, and help to explain specific roles of complement activation in NET-related diseases.

This work presents the investigation on a MEMS based optomechanical transducer for displacements or vibration regarding its cross-sensitivities to multidirectional input excitations. The principle of the optomechanical transducer is based on the modulation of the light flux passing through one static and one movable micromechanical aperture. This kind of transducer is of increasing interest for MEMS sensors since it has inherent benefits and can compete with state-ofthe- art readout concepts regarding its resolution. We have experimentally proven that the sensitivities of the device is 3.3 × 107 V/m in x-direction, 8.23 × 106 V/m in y-direction, while it is negligible in z-direction.

This paper reports a MEMS gradiometer consisting of two independent, laterally oscillating masses on a single chip with integrated optical readout featuring a responsitivity of 35V/T at resonant operation. The symmetrical design of the two masses offers high accuracy and low cost by using conventional MEMS batch fabrication technology. The sensing principle is based on lateral displacement of the masses actuated by Lorentz forces which modulates a light flux passing through a stationary mask and the moving mask integrated in the masses. Phase and intensity detected by photodiodes reveal information about the uniformity of an external applied magnetic field, hence, enables the measurement of gradient-, homogeneous- and offset gradient magnetic fields.

The functional principle of an optical gradient magnetic field sensor consisting of two independent laterally oscillating masses on a single chip is reported. These oscillations are caused by the Lorentz forces resulting from an alternating current through the masses interacting with a static magnetic field. Light is modulated by relative in-plane movement of the masses and a fixed frame and subsequently detected by two photodiodes. Evaluation of magnitude and phase of the output signal reveals information about the uniformity of the magnetic field. The sensor is capable of detecting uniaxially strength and direction of magnetic gradient fields, offset gradient fields and homogeneous fields.

This paper reports a novel 3D-printed MEMS resonant magnetometer with optical readout which features a mechanical conversion of a vertical oscillation into a horizontal one. This demonstrates the advantages of 3D-printing technology in terms of rapid prototyping, low costs and fast product development cycles. In addition, 3D-printing enables ‘true’ three-dimensional MEMS structures in contrast to the traditional MEMS technology which allows only two dimensional structures. The measurement approach comprises a hybrid implementation of an optical modulator, an LED and a photodetector.

This paper reports on a novel, miniaturized magnetomechanical transducer/sensor made of borosilicate glass with wide dynamic range. The prototype is manufactured with laser micromachining and ablation techniques. Compared to state of the art, borosilicate glass substrate offers the highest thermal shock resistance and is best suited for MEMS magnetometers, for aerospace and space applications or magnetic monitoring systems for diagnostics and plasma stability control of nuclear fusion experiments, where thermal shock resistance is a critical requirement.

This paper reports a novel 3D printed MEMS magnetometer with optical readout, which demonstrates the advantages of 3D printing technology in terms of rapid prototyping. Low-cost and fast product development cycles favour 3D printing as an effective tool. Sensitivity measurement with such devices indicate high accuracy and good structural performance, considering material and technological uncertainties. This paper is focusing on the novelty of the rapid, 3D-printing prototyping approach and verification of the working principle for printed MEMS magnetometers.

We report on a new optical sensing principle for measuring the electric field strength based on MEMS technology. This method allows for distortion-free and point-like measurements with high stability regarding temperature. The main focus of this paper rests on an enhanced measurement set-up and the thereby obtained measurement results. These results reveal an improved resolution limit and point to the limitations of the current characterization approach. A resolution limit of 222 V/m was achieved while a further improvement of roughly one order of magnitude is feasible.

Die künstlerische Forschung bietet neue Möglichkeiten und Herausforderungen für akademische Institute im Bereich Jazz und Popularmusik. In diesem Werkstattbericht über das kürzlich abgeschlossene künstlerische Forschungsprojekt Jazz & the City: Identität einer Jazz(haupt)stadt, das vom österreichischen Wissenschaftsfonds FWF im Rahmen des PEEK-Programms gefördert und in Kooperation der Institute für Jazz und Jazzforschung an der Kunstuniversität Graz durchgeführt wurde, wird versucht, eine Perspektive zur Entwicklung der bereits existenten, aber oftmals verdeckt stattfindenden künstlerischen Forschung vor dem Hintergrund der Geschichte dieser beiden Institute herauszuarbeiten. 30.03.2015 | Michael Kahr (Graz)

Cyclopentyl substituted silanetriol can be prepared and isolated. Its condensation yields the corresponding disiloxanetetrol as a primary condensation product. Further condensation leads to the hexameric polyhedral silsesquioxane cage T6. The latter has been mentioned in the literature before however, lacking structural data. All compounds have been characterized with multinuclear NMR spectroscopy and, in addition, the molecular structures have been determined in the case of the disiloxanetetrol and the hexasilsesquioxane via single crystal X-ray diffraction.

This article describes the EGO-TRAP project in which students' visits to the Experimentarium science centre in Copenhagen, Denmark, are structured via their mobile phones. EGO-TRAP, so called because it engages visitors in a personal investigation, has been developed with the aim of creating a structured learning experience for children visiting the Experimentarium. It is designed as a virtual extension of the interactive exhibits at the centre. Although initially directed at young people from high schools in Denmark, a version for primary school children is now being developed. EGO-TRAP uses features often found in computer games and applies them to the concrete physical environment of the science centre. The mobile phone was chosen as the vehicle for creating an interactive story at the science centre because, among other things, mobile phones have the capacity to organise information.

Human β-defensins (hBD) are antimicrobial peptides that curb microbial activity. Although hBD's are primarily expressed by epithelial cells, we show that human platelets express hBD-1 that has both predicted and novel antibacterial activities. We observed that activated platelets surround Staphylococcus aureus (S. aureus), forcing the pathogens into clusters that have a reduced growth rate compared to S. aureus alone. Given the microbicidal activity of β-defensins, we determined whether hBD family members were present in platelets and found mRNA and protein for hBD-1. We also established that hBD-1 protein resided in extragranular cytoplasmic compartments of platelets. Consistent with this localization pattern, agonists that elicit granular secretion by platelets did not readily induce hBD-1 release. Nevertheless, platelets released hBD-1 when they were stimulated by α-toxin, a S. aureus product that permeabilizes target cells. Platelet-derived hBD-1 significantly impaired the growth of clinical strains of S. aureus. hBD-1 also induced robust neutrophil extracellular trap (NET) formation by target polymorphonuclear leukocytes (PMNs), which is a novel antimicrobial function of β-defensins that was not previously identified. Taken together, these data demonstrate that hBD-1 is a previously-unrecognized component of platelets that displays classic antimicrobial activity and, in addition, signals PMNs to extrude DNA lattices that capture and kill bacteria.

This article examines an educational design experiment which aimed to support young people’s involvement and reflection in the exhibition at a Danish science centre. The experiment consisted in the examination of the design and implementation of a mobile phone facilitated narrative, which was planned as a so-called scaffolding remedy in the hands-on based exhibition. The digital narrative, called EGO-TRAP, was developed using Design-Based Research as the overall methodological framework. The study of students’ interactions in the exhibition suggests, among other things, that because of its quality as a digital narrative, EGO-TRAP scaffolds pleasurable engagement and counteracts the tendency of "random button pressing" that often occurs in classical science centre exhibitions. In this connection, the mobile phone plays an essential role due to the fact that it, as the favoured media by the young students, offers an experience which they describe as both personal and flexible.

BackgroundNormal development of the atria requires left-right differentiation during embryonic development. Reduced expression of Pitx2c (paired-like homeodomain transcription factor 2, isoform c), a key regulator of left-right asymmetry, has recently been linked to atrial fibrillation. We therefore systematically studied the molecular composition of left and right atrial tissue in adult murine and human atria.MethodsWe compared left and right atrial gene expression in healthy, adult mice of different strains and ages by employing whole genome array analyses on freshly frozen atrial tissue. Selected genes with enriched expression in either atrium were validated by RT-qPCR and Western blot in further animals and in shock-frozen left and right atrial appendages of patients undergoing open heart surgery.ResultsWe identified 77 genes with preferential expression in one atrium that were common in all strains and age groups analysed. Independent of strain and age, Pitx2c was the gene with the highest enrichment in left atrium, while Bmp10, a member of the TGFβ family, showed highest enrichment in right atrium. These differences were validated by RT-qPCR in murine and human tissue. Western blot showed a 2-fold left-right concentration gradient in PITX2 protein in adult human atria. Several of the genes and gene groups enriched in left atria have a known biological role for maintenance of healthy physiology, specifically the prevention of atrial pathologies involved in atrial fibrillation, including membrane electrophysiology, metabolic cellular function, and regulation of inflammatory processes. Comparison of the array datasets with published array analyses in heterozygous Pitx2c(+/-) atria suggested that approximately half of the genes with left-sided enrichment are regulated by Pitx2c.ConclusionsOur study reveals systematic differences between left and right atrial gene expression and supports the hypothesis that Pitx2c has a functional role in maintaining "leftness" in the atrium in adult murine and human hearts.