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Borehole leakage is an important cause of gas extraction failure. In order to study the influence mechanism of borehole leakage on gas extraction, the borehole air leakage mode was studied, and a mathematical model was established to describe the borehole air leakage of the gas extraction. In addition, this paper also analyzed the change rules of borehole air leakage and gas extraction effect at different borehole sealing depth, negative extraction pressure, degree of fracture development and borehole sealing quality. Research shows that: (1) The hole sealing depth has a certain influence on the borehole air leakage and the gas extraction effect. As the hole sealing depth increased, the borehole air leakage gradually decreased. Moreover, the net amount of gas extraction gradually decreased, but the gas extraction concentration increased to some extent, and the increasing range gradually decreased. The reasonable sealing depth of this coal seam was 9∼10 m. (2) The negative pressure of borehole extraction has little influence on borehole air leakage and gas extraction. With the increase of the negative extraction pressure, the gas extraction net amount and borehole air leakage both increased, but the gas extraction concentration decreased. In the later stage of the gas extraction, the negative pressure can be properly reduced to improve the gas extraction effect. (3) The borehole sealing quality has a great influence on the borehole air leakage and gas extraction effect. With the improvement of the sealing quality, the coal-rock permeability gradually decreased. Moreover, the borehole air leakage significantly reduced, and the gas extraction concentration increased significantly. Therefore, attention should be paid to the selection and improvement of sealing materials and sealing techniques in the actual production so that the airtightness and sealing quality of the hole sealing section can be improved.

ObjectiveThe present study analyzed the effect of hCG trigger day progesterone (P) levels on the live birth rate (LBR) in the gonadotropin-releasing hormone (GnRH) antagonist protocol.Materials and methodsThis study was a single-center retrospective study. In vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) cycles performed from January 2017 to December 2020 were included in the analysis. This study included people with a normal ovarian response to fresh embryo transfer of GnRH antagonist protocols. All cycles were divided into 2 groups by P level on the day of human chorionic gonadotropin (hCG) trigger, P

Soybean meal (SBM) is a premier source of protein for feeding food-producing animals. However, its nutritional value can be compromised by protein oxidation. In this study, a total of 54 sources of solvent extracted SBM (SSBM) and eight sources of mechanically extracted SBM (MSBM), collected from different commercial producers and geographic locations in the United States during the years 2020 and 2021, were examined by chemometric analysis to determine the extent of protein oxidation and its correlation with soybean oil extraction methods and non-protein components. The results showed substantial differences between SSBM and MSBM in the proximate analysis composition, protein carbonyl content, lipidic aldehydes, and antioxidants, as well as subtle differences between 2020 SSBM and 2021 SSBM samples in protein oxidation and moisture content. Correlation analysis further showed positive correlations between protein carbonyl content and multiple lipid parameters, including the ether extract, p-anisidine value, individual aldehydes, and total aldehydes. Among the antioxidants in SBM, negative correlations with protein carbonyl content were observed for total phenolic content and isoflavone glycoside concentrations, but not for Trolox equivalent antioxidant capacity (TEAC), α-tocopherol, and γ-tocopherol. Overall, soybean oil extraction methods, together with other factors such as enzyme treatment and environmental conditions, can significantly affect the proximate analysis composition, the protein and lipid oxidation status, and the antioxidant profile of SBM. Lipidic aldehydes and phenolic antioxidants play counteracting roles in the oxidation of soy protein. The range of protein carbonyl content measured in this study could serve as a reference to evaluate the protein quality of SBM from various sources used in animal feed.

A dynamic disturbance will induce cracks around the tunnel in tunnel blasting or shield construction. To investigate the overall stability of cracks with various angles during a fixed borehole (round hole explosion) blasting, models containing an individual crack with different angles were introduced for simulation research. The research set up a thin sheet model with a length of 350 mm and a width of 150 mm, with a 7 mm diameter hole and a pre-existing crack of 75 mm and 5 mm in the middle. The evolution of the stress wave propagation model and the crack propagation model were simulated using the AUTODYN software. And in this study, the theory of stress wave is used to creatively explain the dynamic load under the action of formation and reasons for the danger zone. The results indicate that pre-existing cracks from different angles will have an impact on the blast hole and the new cracks generated around itself. At 45–90°, pre-existing cracks will direct reflected stress waves to promote some cracks around the hole to have faster growth rates than others, and these special cracks with faster growths and longer lengths will more easily connect with the free surface or other cracks, resulting in overall instability. And these conditions are consistent with the prediction made by the stress wave propagation simulation study. The research results have certain guiding significance for the stability analysis and hazardous area prediction of tunnel blasting with existing cracks.

We developed a Dual-PPACs detector for fast neutron measurements that consists of two sets of PPAC: conventional PPAC and fission PPAC. A238U(U3O8) coating is placed in the fission PPAC’s anode, which is used as the neutrons conversion layer. An experiment was performed to measure neutron time-of-flight (TOF) in which 252Cf spontaneous fission source was used. An excellent time resolution of 164ps has been observed at 6 mbar in isobutene gas. With the excellent time resolution of Dual-PPACs detector, exact neutron energy can be extracted from the timing measurement. The experimental detection efficiency was 1.9 × 10−7, consistent with the efficiency of 2.5 × 10−7 given by a Geant4 simulation. Ultimately, the results show that the Dual-PPACs detector is a suitable candidate for measuring fast neutrons in the future CiADS system.

Abstract Aqueous ammonium ion batteries are regarded as eco-friendly and sustainable energy storage systems. And applicable host for NH4 + in aqueous solution is always in the process of development. On the basis of density functional theory calculations, the excellent performance of NH4 + insertion in Prussian blue analogues (PBAs) is proposed, especially for copper hexacyanoferrate (CuHCF). In this work, we prove the outstanding cycling and rate performance of CuHCF via electrochemical analyses, delivering no capacity fading during ultra-long cycles of 3000 times and high capacity retention of 93.6% at 50 C. One of main contributions to superior performance from highly reversible redox reaction and structural change is verified during the ammoniation/de-ammoniation progresses. More importantly, we propose the NH4 + diffusion mechanism in CuHCF based on continuous formation and fracture of hydrogen bonds from a joint theoretical and experimental study, which is another essential reason for rapid charge transfer and superior NH4 + storage. Lastly, a full cell by coupling CuHCF cathode and polyaniline anode is constructed to explore the practical application of CuHCF. In brief, the outstanding aqueous NH4 + storage in cubic PBAs creates a blueprint for fast and sustainable energy storage.

ObjectiveTo compare cumulative live birth rates and perinatal outcomes of young IVF/ICSI patients with low anti-Mullerian hormone (AMH) levels on a gonadotropin-releasing hormone antagonist (GnRH-ant) regimen with those on a high progesterone state of ovulation (PPOS) regimen.MethodsWe retrospectively analyzed 798 patients who underwent in vitro fertilization (IVF) or intracytoplasmic sperm microinjection (ICSI) between January 2015 and December 2020 at the Third Affiliated Hospital of Zhengzhou University. A total of 798 cycles of complete clinical data from patients who underwent in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) at the Reproductive Medicine Center of Zhengzhou University Hospital between January 2015 and December 2020 and were eligible for AMH < 1.2 ng/ml at age < 35 years, Group A1: very low AMH levels (AMH < 0.5 ng/mL) and GnRH antagonist regimen; Group A2, very low AMH level (AMH < 0.5 ng/mL) and PPOS regimen; Group B1, low AMH level (0.5 ng/mL ≤ AMH < 1.2 ng/mL) and GnRH antagonist regimen; and Group B2, low AMH level (0.5 ng/mL ≤ AMH < 1.2 ng/mL), and the PPOS regimen.ResultsAt very low levels of AMH (< 0.5 ng/mL), the CLBR of the GnRH antagonist regimen was not significantly different from that of the PPOS regimen (P > 0.05), at 0.5 ng/mL ≤ AMH < 1.2 ng/mL. Statistics showed that the CLBR of the GnRH antagonist regimen was significantly higher than that of the PPOS regimen (49.7% vs. 35.7%, P=0.002). Logistic regression analysis showed that in Group A: the younger the female partner, the higher the CLBR (OR = 0.972, 95% CI = 0.923–1.042, P = 0.022), and the more the AFC, the higher the CLBR (OR = 1.166, 95% CI = 1.091–1.336, P < 0.001). Group B: the higher the number of good-quality embryos, the higher the CLBR (OR = 2.227, 95% CI = 1.869–2.654, P < 0.001). Compared with PPOS regimens, the antagonist regimen was able to increase the CLBR. The analysis of Group A showed that the antagonist regimen had a shorter TTP than the PPOS regimen (P < 0.001); however, the PPOS regimen had a lower cost of ovulation (4311.91 vs. 4903.81, P = 0.023). The antagonist regimen in Group B had a shorter TTP than the PPOS regimen, and there was no significant difference in the cost of ovulation. In the analysis of perinatal outcomes, there were no statistically significant differences in preterm birth, low birth weight, very low birth weight, and pregnancy complications among the four groups.ConclusionYoung patients with very low AMH levels (< 0. 5 ng/mL), the GnRH antagonist regimen was comparable to the PPOS regimen in CLBR outcomes; the antagonist regimen shortens the time to clinical pregnancy, and the PPOS regimen is more cost-effective. In young patients with low AMH levels of 0.5 ng/mL and

IntroductionHyperprogressive disease (HPD) is a paradoxically rapid disease progression during or shortly after antitumor treatment, especially immune checkpoint inhibitors (ICIs). Various diagnosis criteria of HPD cause heterogeneous incidence rates in different clinical research, and there is no consensus on potential risk factors associated with HPD occurrence. Hence, we aimed to summarize incidence of HPD in ICI treatment for solid tumors. Clinicopathological factors associated with HPD are also analyzed.MethodsClinical studies about HPD during/after ICI treatment of solid malignancies are included. Pubmed, Embase, and Cochrane library were searched for eligible studies published before October 7. The Newcastle–Ottawa scale was used to assess the quality of the included studies. Random effect and fixed effect models were, respectively, used for pooling incidence of HPD and analysis of risk factors for HPD. Heterogeneity, subgroup analysis, and publication bias were also analyzed. All meta-analysis was performed via R software (y -40v4.0.2).ResultsForty-one studies with 6009 patients were included. The pooled incidence of HPD was 13.2% (95% CI, 11.2%–15.4%). Head and neck cancer (HNC) had the highest incidence of HPD (18.06%), and melanoma had the lowest (9.9%). Tumor types (P = .0248) and gender ratio (P = .0116) are sources of heterogeneity of pooled incidence of HPD. For five clinicopathological factors associated with HPD, only programmed cell death protein 1 ligand 1 (PD-L1) positivity was a preventive factor (odds ratio = 0.61, P 2 (OR = 2.38, P

Abstract Many very deep neural networks are proposed to obtain accurate super‐resolution reconstruction of remote sensing images. However, the deeper the network for image SR is, the more difficult it is to train. The low‐resolution inputs and features contain abundant low‐frequency information and noise, which are treated equally as the high‐frequency information to across the network. To solve these problems, a novel single‐image super‐resolution algorithm named pre‐training of gated convolution neural network (PGCNN) is proposed for remote sensing images. The proposed PGCNN consists of several residual blocks with long skip connections. Each residual block contains an additional well‐designed gated convolution unit, which provides different weights to high‐frequency information and low‐frequency information to control the transmission of information, making the main network focus on learning high‐frequency information. Compared with several state‐of‐the‐art methods, experimental results on the remote sensing datasets (SIRI‐WHU, NWPU‐RESISC45, RSSCN7 and UC‐Merced‐Land‐Use) show that the proposed PGCNN has the accuracy and visual improvements.

Abstract With the rapid development of the IoT, smart home plays an increasingly important role in daily life. At the same time, privacy issues have gradually aroused the concern of researchers. Past research proves that there are privacy risks when people use smart home devices and some protection methods are proposed to protect people's privacy. However, attackers can still infer people's behaviour and activities through analysing the wireless traffic of smart home devices. Herein, an attack method under complicated WiFi scenarios is demonstrated, including different locations, different buildings and different networks. In addition, a new protection method, Air‐Padding, is developed, which can change the traffic patterns of smart home devices through injecting constructed packets into the link between devices and routers. The results prove that Air‐Padding can prevent people's behaviour and activities from being analysed and inferred.

Modern cryptography attributes the security of a cryptographic system to the security of the key. How to securely distribute the key has always been a bottleneck in key management. This paper proposes a secure group key agreement scheme for multiple parties using a multiple twinning superlattice physical unclonable function (PUF) that can be synchronized. By sharing the challenge and helper data among multiple twinning superlattice PUF holders, the scheme employs a reusable fuzzy extractor to obtain the key locally. Moreover, adopting public-key encryption encrypts public data for establishing the subgroup key, which provides independent communication for the subgroup. At the same time, when the subgroup membership changes, the public key encrypts new public data to update the subgroup key, forming scalable group communication. This paper also presents a cost and formal security analysis, which shows that the proposed scheme can achieve computational security by applying the key obtained by the computationally secure reusable fuzzy extractor to the EAV-secure symmetric-key encryption, which has indistinguishable encryption in the presence of an eavesdropper. Additionally, the scheme is secure against physical attacks, man-in-the-middle attacks, and machine learning modeling attacks.

Reversible data hiding in encrypted images (RDH-EI) is instrumental in image privacy protection and data embedding. However, conventional RDH-EI models, involving image providers, data hiders, and receivers, limit the number of data hiders to one, which restricts its applicability in scenarios requiring multiple data embedders. Therefore, the need for an RDH-EI accommodating multiple data hiders, especially for copyright protection, has become crucial. Addressing this, we introduce the application of Pixel Value Order (PVO) technology to encrypted reversible data hiding, combined with the secret image sharing (SIS) scheme. This creates a novel scheme, PVO, Chaotic System, Secret Sharing-based Reversible Data Hiding in Encrypted Image (PCSRDH-EI), which satisfies the (k,n) threshold property. An image is partitioned into N shadow images, and reconstruction is feasible when at least k shadow images are available. This method enables separate data extraction and image decryption. Our scheme combines stream encryption, based on chaotic systems, with secret sharing, underpinned by the Chinese remainder theorem (CRT), ensuring secure secret sharing. Empirical tests show that PCSRDH-EI can reach a maximum embedding rate of 5.706 bpp, outperforming the state-of-the-art and demonstrating superior encryption effects.

The rise of migrant workers has been a unique social phenomenon as China goes through industrialization, urbanization, and modernization. They are a special social group formed during the economic and social transition of the country. Migration of rural labor has pushed China on its new path toward industrialization and urbanization. Because of the urban-rural dual system of the country, however, it is difficult for migrant workers to be fully integrated into host cities, making them susceptible to negative emotions and mental health issues. Therefore, their mental health is an issue of great volume in the domains of social undertakings, people's livelihood, and public health. However, existing studies have paid limited attention to the psychological profile of migrant workers and even less to the interplays among their social capital, social integration, and mental health. Targeting China's internal migrant workers, this article tapped the interactions among their social integration, social capital, and mental health with a sample of the cross-sectional data from the China Labor Dynamics Survey (CLDS) in 2018. Multi-group structural equation modeling (SEM) was employed to test the moderating action of age by analyzing whether the mediation model differed significantly in the paths among young, middle-aged, and older migrant workers. The SEM based on bootstrapping suggested that, after controlling for the influence of gender, education, marital status, personal annual income, employer type, and self-rated health, migrant workers' social capital positively affect their mental health in a significant way, with social integration playing a mediating role. In terms of age difference, middle-aged migrant workers were more subject to the aforementioned mechanism than young ones, and young migrant workers were more affected by the mechanism than older ones. This study revealed different psycho-social interplays among social capital, social integration, and mental health across young, middle-aged, and elderly migrant workers. The findings could serve as an important theoretical reference and as practical guidance for improving policies concerning migrant workers' mental health and social benefits in the context of economic transition.

BackgroundAccumulating evidence suggests that dysregulation of Chordin-like 1 (CHRDL1) is associated with malignant biological behaviors in multiple cancers. However, the exact function and molecular mechanism of CHRDL1 in oral squamous cell carcinoma (OSCC) remain unclear.MethodsThe expression levels of CHRDL1 in OSCC tissues and CAL27 cells were determined by RT-qPCR. Immunohistochemical staining was applied to detect CHRDL1 protein expression in sample tissues from OSCC patients. Gain of function and knockdown by lentivirus were further used to examine the effects of CHRDL1 on cell proliferation, migration, invasion, and adhesion in OSCC. Tail vein injection of CAL27 cells with dysregulated CHRDL1 expression was further used to examine the effect of CHRDL1 on lung colonization. RNA sequencing was performed to explore the molecular mechanisms of CHRDL1 that underlie the progression of OSCC.ResultsCHRDL1 was significantly downregulated in OSCC tissues and CAL27 cells compared to controls. CHRDL1 knockdown enhanced migration, invasion, adhesion, and EMT, but not proliferation, in CAL27 cells. Overexpression of CHRDL1 had the opposite effects. Moreover, CHRDL1 was proven to inhibit tumor metastasis in vivo. Mechanistically, MAPK signaling pathway components, including ERK1/2, p38, and JNK, were found to regulate the malignant biological behaviors of CAL27 cells.ConclusionsOur results suggest that CHRDL1 has an inhibitory effect on OSCC metastasis via the MAPK signaling pathway, which provides a new possible potential therapeutic target against OSCC.

Population mobility has been one of the most basic social characteristics of China’s reform and opening up for more than 40 years. As the main labor force in Chinese cities, young migrants have made major contributions toward China’s economic miracle as the country has experienced rapid industrialization and urbanization. However, frequent mobility has caused an imbalanced social mentality in young migrants and often leads to issues with social integration, which has made this group more vulnerable with respect to their health. This study used the 2013 and 2015 Chinese General Social Survey (CGSS) data of 1,007 young migrants to investigate social mentality mediating the linkage between social integration and health among young migrants. Additionally, to probe the moderating role of gender, multi-group structural equation modeling was applied to test if the pathways in the mediation model differed between young male and female migrants in a significant way. The results suggested that after controlling for the influence of age, marital status, education, and personal annual income, social integration positively impacted the health of young migrants in a significant way; social mentality mediated the action of social integration on this group’s health; and with respect to gender difference, on the three paths of social integration affecting health, social integration affecting social mentality, and social mentality affecting health, young male migrants were more affected than young female migrants. The findings of this study could help improve gender-specific policies on the health of the floating population and offer important theoretical reference and practical suggestions for future research.

PurposeBreast cancer (BC) has been extensively and deeply studied as the number one malignant tumor in women, but its status in male patients, especially in male metastatic patients, is rarely reported. Thus, this study aimed to explore the prognosis and risk factors of male BC with bone metastasis.Patients and MethodsWe searched the Surveillance, Epidemiology, and End Results (SEER) database to identify all patients diagnosed with male BC with bone metastasis from 2010 to 2016. Risk factors of overall survival (OS) and cancer-specific survival (CSS) were analyzed by univariable and multivariable Cox analyses. We also drew Kaplan–Meier plots to show the correlation between independent risk factors and survival.ResultsA total of 207 male BC patients with bone metastasis were included for analysis. Approximately one-third of patients also had lung metastasis. Luminal A subtype comprised 58.5% of the overall patient population. These patients had a poor prognosis, with 3-year OS and CSS rates, 36.7% and 39.5%, respectively. Further analysis revealed that age ≤60 years old, luminal A or B, and surgery were independent predictors of prolonged OS and CSS. On Cox multivariable analysis, brain metastasis was associated with OS and not CSS.ConclusionWe identified four independent factors associated with prognosis in male BC patients with bone metastasis, namely age, tumor subtype, surgery, and brain metastasis. Knowing these risk factors will help clinicians make more appropriate treatment plans.

BackgroundWith the increasing use of frozen embryo transfer (FET), the best endometrial preparation protocol is continuously being discussed. The hormone replacement therapy (HRT) cycle and letrozole-induced ovulation (L-OI) cycle are available protocols for patients with abnormal ovulation. Previous comparisons of the two protocols have focused on pregnancy outcomes, with less attention to perinatal outcomes, and population heterogeneity was large; thus, convincing conclusions about which protocol is more appropriate could not be drawn.MethodsWe performed a retrospective cohort study using propensity score matching (PSM) analysis for a population of patients undergoing FET cycles in the reproductive center of the Third Affiliated Hospital of Zhengzhou University from January 2016 to September 2020. The main outcome measures were clinical pregnancy rate, live birth rate, very preterm delivery (VPTD), preterm delivery (PTD), low birth weight (LBW), macrosomia, small for gestational age (SGA), large for gestational age (LGA), hypertensive disorders of pregnancy (HDP), gestational diabetes mellitus (GDM), premature rupture of membranes (PROM), placenta previa, and congenital abnormality.ResultsA total of 8010 women were enrolled. Due to the large heterogeneity among the patients, we conducted 1:1 PSM, and 1461 women matched in each group. Compared with the HRT group, the L-OI group had a smaller proportion of thin endometrium (27.38% vs. 41.07%) and thicker endometrium on the day of embryo transfer (9.63 ± 1.82 vs. 8.91 ± 1.38). There were no significant differences in clinical pregnancy rate, early abortion rate or live birth rate between the groups. There was no significant difference in perinatal outcomes of singleton live birth, including VPTD, PTD, postterm delivery, LBW, macrosomia, SGA, LGA, GDM, HDP, placenta previa, and congenital malformation.ConclusionFor women with abnormal ovulation, the pregnancy and perinatal outcomes of HRT and L-OI protocols are reassuring. It seems that both protocols are safe and effective for endometrial preparation in frozen-thawed embryo transfer in the clinic.

Abstract Background Accidental strangulation due to scarf getting caught in the wheels of a vehicle or machine was called “Isadora Duncan Syndrome” or “Long Scarf Syndrome”. Survival of concomitant fracture dislocation of cervical spine and oesophageal perforation following Long Scarf Syndrome was rarely described and medium-term follow-up for this lesion has not been reported. Case presentation We present a 39-year-old female who suffered accidental strangulation caused by the scarf around her neck getting trapped in the wheels of the a vehicle and was referred to our hospital forty days post injury. The CT examination showed a fracture dislocation at C5/6 levels with complete dissociation of the supporting structures. She developed paravertebral abscesses, cutaneous fistulas and oesophageal perforation confirmed by oesophagoscope. The patient was treated conservatively because of poor general condition and inappropriate initial treatment. Halo-vest was used to immobilize the cervical spine. The oesophagus-cutaneous fistula was managed with enteral tube feeding and repeated local care. The patient survived despite such severe injury. Nine months after the injury, the oesophageal perforation closed spontaneously and fixed malunion of the cervical spine was achieved. Six-year follow-up demonstrated that the patient survived with complete C5 tetraplegia. Literature associated with this lesion was reviewed and factors contributing to the survival were discussed. Conclusions Concomitant fracture dislocation of cervical spine and oesophageal perforation following Long Scarf Syndrome is extremely rare with high risk of mortality. Though surgical intervention is always necessary, the optimal management for this kind of lesion should be made on an individual basis through a multidisciplinary approach.

Strong magnetic interfacial coupling in van der Waals heterostructures provides a new platform for discovering novel physics and effects. Here, the authors report the formation of skyrmion lattice in the WTe2/Fe3GeTe2 van der Waals heterostructure and a Dzyaloshinskii–Moriya interaction with a large energy density of 1.0 mJm−2.

Considering serious concrete diseases from tunnel lining structure, the pre-suspended nanomaterials combined with superfine admixtures (nano-SA) are used to improve mechanical properties and durability of concrete. Fly ash combined with ultrafine particle microspheres were selected as composite admixtures based on comprehensive performance and application research analysis on the mineral nanomaterial admixtures. Compressive strength and flexural strength for the mortar specimens at different ages were investigated by the single or composite incorporation of the silica fume and the other nanomaterials with the different physical properties. The nano-SA determined by experiment was applied to regulate strength and durability of the concrete. The Scanning Electron Microscopy (SEM) was used to analyze microstructure of concrete with different prescriptions under freeze–thaw cycles. The effects of the nano-SA acting on concrete compactness were studied. The pre-suspended nanomaterial combined with superfine admixture is used for the experimental shotcrete of tunnel. The experimental results indicate that the nano-SA not only improves the workability and compressive strength of the shotcrete but also significantly reduces the rebound rate of the shotcrete. The rebound amount of the shotcrete incorporated with the nano-SA is decreased approximately 30% than without the nano-SA. It proves the excellent performance of the nano-SA and obvious economic benefits.

Meesmann epithelial corneal dystrophy (MECD) is a rare dominantly inherited disorder that is characterized by corneal epithelial microcysts and is associated with mutations in the keratin 3 (KRT3) and keratin 12 (KRT12) genes. In this study, we report a novel mutation in the KRT12 gene in a Vietnamese pedigree with MECD. Slit-lamp examination was performed on each of the 7 recruited members of a Vietnamese family to identify characteristic features of MECD. After informed consent was obtained from each individual, genomic DNA was isolated from saliva samples and screening of KRT3and KRT12 genes was performed by Sanger sequencing. The proband, a 31-year-old man, complained of a 1-year history of eye irritation and photophobia. Slit-lamp examination revealed intraepithelial microcysts involving only the corneal periphery in each eye with clear central corneas and no stromal or endothelial involvement. Three family members demonstrated similar intraepithelial microcysts, but with diffuse involvement, extended from limbus to limbus. Sanger sequencing of KRT3 (exon 7) and KRT12 (exons 1 and 6) in the proband revealed a novel heterozygous KRT12 variant (c.1273G>A [p.Glu425Lys]) that was present in the three affected family members but was absent in the three family members with clear corneas. This study is the first report of a Vietnamese family affected with MECD, associated with an atypical peripheral corneal epithelial phenotype in the proband and a novel mutation in KRT12.

We have developed a position-sensitive Parallel Plate Avalanche Counter (PPAC) to detect the fission fragments and reconstruct the fission reaction plane in the experiment of studying nuclear equation of state (nEOS) by means of heavy ion collision (HIC). This experiment put forward high requirements for the performances of PPAC, such as the time resolution, efficiency and position resolution. According to these requirements we designed the PPAC with an active area of 240 mm × 280 mm working at low gas pressure. The results show that time resolution could be less than 300 ps. Position resolution is consistent with the theoretical calculation about 1.35 mm. Detection efficiency could be approaching 100% gradually with the voltage increasing in different gas pressures. The performances of PPAC have also been verified in beam experiment. Each set of anode wires can be accurately separated in the position spectrum. In the beam experiment, we also got the back-to-back correlation of fission fragments which is one of the direct signals characterizing binary decay. Keywords: PPAC, nEOS, Time resolution, Position resolution, Efficiency

Skyrmions are objects with whirled magnetization protected by their topology that can be created by different means, however, without control of their position. Here, the authors present a method exploiting x-rays to create skyrmions at the beam position allowing for creation of artificial skyrmion lattices.

In this paper, the synchronous control problem of photovoltaic grid-connected inverter with nonlinear impulse perturbation is studied. The mathematical model of photovoltaic grid-connected converter is established by using Kirchhoff's law. The nonlinear impulse perturbation characteristics of grid-connected current are studied and analyzed. Controllers are designed to suppress nonlinear impulse perturbation. The effectiveness of the controller is verified by Lyapunov stability theory. Finally, the simulation design is carried out by simulink in Matlab and the test platform is constructed. In this paper, simulation verification and data comparison are carried out by using various control methods for suppressing nonlinear impulse perturbation of grid-connected currents. Compared with PI controller, PR controller and other controller, the output harmonic content of photovoltaic inverter can be controlled within 1%, which can effectively reduce the harmonic content. The power quality of grid-connected current is improved. The feasibility and effectiveness of the control strategy proposed are verified by simulation and experiments.

Positional modulation (PM) has been introduced recently where a given modulation pattern can only be received at certain desired positions. To achieve it, the multi-path effect is exploited for positional modulation with the aid of metasurface acting as a low-cost flexible reflecting surface. In this paper, sparse antenna array based positional modulation design is proposed for the first time; to reduce the implementation complexity of the metasurface, the number of active units is also minimised for a given PM design requirement. Design examples are provided to show the effectiveness of the proposed design.

A high-precision networking method for a modular deployable antenna with ultramultiple modules is proposed to solve the problem of achieving an accurate formation of the structure of a large-aperture modular deployable space antenna. In accordance with the general requirements for the deployment surface of deployable antennas, the structure composition and deployment principle of a deployable antenna are analyzed, and the topological law of a hexagonal prism modular structure is expounded. On the basis of basic theories of differential geometry, analytical geometry, and robotics, the mathematical model of an envelope circle and that of its center are established. Then, a networking model of structure that includes 61 modules in the fully deployed state is established. Finally, the established model is verified via a numerical simulation method, and the influence law of structural parameters on networking characteristics is analyzed and studied. Simulation results show that the networking method can realize an accurate connection of multiple modules. The networking method for the structure of a modular deployable antenna proposed in this study can provide a reference for research on basic theory of a deployable antenna’s mechanism and related engineering applications.

ObjectiveThe purpose of this study was to explore the effects of different methods of obtaining sperm for intracytoplasmic sperm injection (ICSI) cycles on the live birth rate (LBR) and neonatal outcomes.MethodsThis was a single-center retrospective cohort study conducted from January 2016 to December 2019. A total of 3557 ICSI cycles were included in the analysis, including 540 cycles in the surgically acquired sperm group and 3017 cycles in the ejaculated sperm group. The main outcome measure was the LBR.ResultsThe clinical pregnancy rate in the surgically acquired sperm group was 69.4%, which was significantly higher than the 59.7% clinical pregnancy rate in the ejaculated sperm group (P=0.01). The LBR of the surgically acquired sperm group was significantly higher than that of the ejaculated sperm group (63.1% vs. 51.2%, P

The usage of mobile phones has undergone tremendous growth in the past decades. Large amounts of mobile-phone signaling data (MSD) are generated while using various mobile phone applications. The large-scale MSD presents opportunities for transport planners to utilize it for better planning and management of the transportation system. In this paper, we use MSD to analyze subway passengers’ travel behavior and extract their complete travel trajectories. The complete travel trajectories of subway passengers include their trajectories, both inside and outside the subway system. In the first stage, the MSD from the subway base stations is selected, sorted by time, and the rough trajectory in the subway system is extracted. The ground base stations around the subway station are then considered to correct the boarding and alighting subway stations in order to obtain a more detailed trajectory. In the second stage, the service range of the base station is determined according to the Thiessen polygon, and a temporal dynamic threshold is proposed to extract the passenger’s stop point outside the subway system. Finally, the complete trajectories of subway passengers are obtained. The proposed algorithms are verified using a set of MSD collected in Suzhou, China. The results show that the proposed algorithms can effectively extract the complete travel trajectory of subway passengers.

Network security situation awareness (NSSA) is an integral part of cybersecurity defense, and it is essential for cybersecurity managers to respond to increasingly sophisticated cyber threats. Different from traditional security measures, NSSA can identify the behavior of various activities in the network and conduct intent understanding and impact assessment from a macro perspective so as to provide reasonable decision support, predicting the development trend of network security. It is a means to analyze the network security quantitatively. Although NSSA has received extensive attention and exploration, there is a lack of comprehensive reviews of the related technologies. This paper presents a state-of-the-art study on NSSA that can help bridge the current research status and future large-scale application. First, the paper provides a concise introduction to NSSA, highlighting its development process. Then, the paper focuses on the research progress of key technologies in recent years. We further discuss the classic use cases of NSSA. Finally, the survey details various challenges and potential research directions related to NSSA.

Asobara japonica (Hymenoptera: Braconidae) is an endoparasitoid wasp that can successfully parasitize a wide range of host species across the Drosophila genus, including the invasive crop pest Drosophila suzukii. Parasitoids are capable of regulating the host metabolism to produce the nutritional metabolites for the survival of their offspring. Here, we intend to investigate the metabolic changes in D. melanogaster hosts after parasitization by A. japonica, using the non-targeted LC-MS (liquid chromatography-mass spectrometry) metabolomics analysis. In total, 3043 metabolites were identified, most of which were not affected by A. japonica parasitization. About 205 metabolites were significantly affected in parasitized hosts in comparison to non-parasitized hosts. The changed metabolites were divided into 10 distinct biochemical groups. Among them, most of the lipid metabolic substances were significantly decreased in parasitized hosts. On the contrary, most of metabolites associated with the metabolism of amino acids and sugars showed a higher abundance of parasitized hosts, and were enriched for a wide range of pathways. In addition, eight neuromodulatory-related substances were upregulated in hosts post A. japonica parasitization. Our results reveal that the metabolites are greatly changed in parasitized hosts, which might help uncover the underlying mechanisms of host manipulation that will advance our understanding of host–parasitoid coevolution.

A private intersection-sum (PIS) scheme considers the private computing problem of how parties jointly compute the sum of associated values in the set intersection. In scenarios such as electronic voting, corporate credit investigation, and ad conversions, private data are held by different parties. However, despite two-party PIS being well-developed in many previous works, its extended version, multi-party PIS, has rarely been discussed thus far. This is because, depending on the existing works, directly initiating multiple two-party PIS instances is considered to be a straightforward way to achieve multi-party PIS; however, by using this approach, the intersection-sum results of the two parties and the data only belonging to the two-party intersection will be leaked. Therefore, achieving secure multi-party PIS is still a challenge. In this paper, we propose a secure and lightweight multi-party private intersection-sum scheme called SLMP-PIS. We maintain data privacy based on zero sharing and oblivious pseudorandom functions to compute the multi-party intersection and consider the privacy of associated values using arithmetic sharing and symmetric encryption. The security analysis results show that our protocol is proven to be secure in the standard semi-honest security model. In addition, the experiment results demonstrate that our scheme is efficient and feasible in practice. Specifically, when the number of participants is five, the efficiency can be increased by 22.98%.

This paper reviews the artificial intelligent algorithms in engine management. This study provides a clear image of the current state of affairs for the past 15 years and provides fresh insights and improvements for future directions in the field of engine management. The scope of this paper comprises three main aspects to be discussed, namely, engine performance, engine control, and engine diagnosis. The first is associated with the need to control the basic characteristics that prove that the engine is working properly, namely, emission control and fuel economy. Engine control refers to the ability to identify and fulfill the requirements derived from performance, emissions, and durability. In this part, hybrid electric vehicle (HEV) application and transient operations are discussed. Lastly, engine diagnosis entails assessment techniques that can be used to identify problems in the engine and solve them accordingly. In this part, misfire detection, knock detection, and intake system leakage will be evaluated. In engine performance, neural network algorithms provide efficient results in terms of emission control and fuel economy as the requirements are easily achievable. However, when it comes to engine control and diagnosis, the fuzzy logic rule with its strong robustness and neural networks algorithms are limited in efficiency due to the complex nature of the processes and the presence of big data, for instance, in HEVs in engine control. That has brought forward the usage of reinforcement learning and novel machine learning algorithms in recent years to maximize efficiency in engine control and engine diagnosis, as highlighted in the following part. The PRISMA methodology was used to justify the reference selection in this review.

ObjectiveTo explore whether elevated anti-Müllerian hormone (AMH) levels affect the rate of preterm birth (PTB) among PCOS patients with different BMIs.MethodsIn this retrospective cohort study, patients with PCOS who had undergone IVF/ICSI from January 2017 to December 2019 were included for potential evaluation. A total of 2368 singleton live births from PCOS patients were included. According to the BMI, all the PCOS patients were divided into two groups: BMI6.45 ng/ml (n=325). Additionally, 1029 cycles of patients with a BMI≥24 kg/m2 were grouped according to the serum AMH level: ① 6.45 ng/ml (n=268), with 6.45, AOR (95% CI)=0.78 (0.35-1.73), P=0.54). Regarding PCOS patients with a BMI ≥24 kg/m2, compared with the PTB rate of the AMH 6.45 ng/ml group was significantly higher (OR=2.47; 95% CI=1.34-4.55). After multiple logistic regression analysis, the risk of PTB in the AMH>6.45 ng/ml group was 2.1 times that in the AMH6.45 ng/ml (75th percentile) is an independent risk factor for PTB.

N6-methyladenosine (m6A) RNA modification is a reversible mechanism that regulates eukaryotic gene expression. Growing evidence has demonstrated an association between m6A modification and tumorigenesis and response to immunotherapy. However, the overall influence of m6A regulators on the tumor microenvironment and their effect on the response to immunotherapy in lung adenocarcinoma remains to be explored. Here, we comprehensively analyzed the m6A modification patterns of 936 lung adenocarcinoma samples based on 24 m6A regulators. First, we described the features of genetic variation in these m6A regulators. Many m6A regulators were aberrantly expressed in tumors and negatively correlated with most tumor-infiltrating immune cell types. Furthermore, we identified three m6A modification patterns using a consensus clustering method. m6A cluster B was preferentially associated with a favorable prognosis and enriched in metabolism-associated pathways. In contrast, m6A cluster A was associated with the worst prognosis and was enriched in the process of DNA repair. m6A cluster C was characterized by activation of the immune system and a higher stromal cell score. Surprisingly, patients who received radiotherapy had a better prognosis than patients without radiotherapy only in the m6A cluster C group. Subsequently, we constructed an m6A score model that qualified the m6A modification level of individual samples by using principal component analysis algorithms. Patients with high m6A score were characterized by enhanced immune cell infiltration and prolonged survival time and were associated with lower tumor mutation burden and PD-1/CTLA4 expression. The combination of the m6A score and tumor mutation burden could accurately predict the prognosis of patients with lung adenocarcinoma. Furthermore, patients with high m6A score exhibited greater prognostic benefits from radiotherapy and immunotherapy. This study demonstrates that m6A modification is significantly associated with tumor microenvironment diversity and prognosis. A comprehensive evaluation of m6A modification patterns in single tumors will expand our understanding of the tumor immune landscape. In addition, our m6A score model demonstrated that the level of immune cell infiltration plays a significant role in cancer immunotherapy and provides a basis to increase the efficiency of current immune therapies and promote the clinical success of immunotherapy.

In this study, we performed the first nuclear proteome analysis of wheat developing grains under water deficit by using a label-free based quantitative proteomic approach. In total, we identified 625 unique proteins as differentially accumulated proteins (DAPs), of which 398 DAPs were predicted to be localized in nucleus. Under water deficit, 146 DAPs were up-regulated and mainly involved in the stress response and oxidation-reduction process, while 252 were down-regulated and mainly participated in translation, the cellular amino metabolic process, and the oxidation-reduction process. The cis-acting elements analysis of the key nuclear DAPs encoding genes demonstrated that most of these genes contained the same cis-acting elements in the promoter region, mainly including ABRE involved in abscisic acid response, antioxidant response element, MYB responsive to drought regulation and MYC responsive to early drought. The cis-acting elements related to environmental stress and hormones response were relatively abundant. The transcription expression profiling of the nuclear up-regulated DAPs encoding genes under different organs, developmental stages and abiotic stresses was further detected by RNA-seq and Real-time quantitative polymerase chain reaction, and more than 50% of these genes showed consistency between transcription and translation expression. Finally, we proposed a putative synergistic responsive network of wheat nuclear proteome to water deficit, revealing the underlying mechanisms of wheat grain nuclear proteome in response to water deficit.

ObjectiveTo compare the cumulative live birth rate (CLBR) of the progestin-primed ovarian stimulation (PPOS) protocol with that of the flexible GnRH antagonist protocol in patients with poor prognosis diagnosed per the POSEIDON criteria.MethodsThis was a retrospective cohort study. Low-prognosis women who underwent IVF/ICSI at the Reproductive Center of Third Affiliated Hospital of Zhengzhou University between January 2016 and January 2019 were included according to the POSEIDON criteria. The CLBR was the primary outcome of interest. The secondary outcome measures were the numbers of oocytes retrieved, 2PN embryos, available embryos and time to live birth.ResultsA total of 1329 women met the POSEIDON criteria for analysis. For POSEIDON group 1, the dosage of gonadotropin (Gn) was higher in the PPOS group than in the GnRH antagonist group (2757.3 ± 863.1 vs 2419.2 ± 853.1, P=0.01). The CLBR of the PPOS protocols was 54.4%, which was similar to the rate of 53.8% in the GnRH antagonist group. For POSEIDON group 2, the number of available embryos was higher in the PPOS group (2.0 ± 1.7 vs 1.6 ± 1.4, P=0.02) than in the GnRH antagonist group. However, the CLBRs of the two groups were similar (18.1% vs 24.3%, P=0.09). For POSEIDON groups 3 and 4, there were no statistically significant differences in the number of oocytes retrieved, 2PN, available embryos or CLBR between the two protocols. After adjustments for confounding factors, the CLBR remained consistent with the unadjusted rates. In the POSEIDON group 1 population, the GnRH antagonist protocols had a shorter time to live birth (P=0.04).ConclusionFor low-prognosis patients diagnosed per the POSEIDON criteria, the CLBR of PPOS protocols is comparable to that of GnRH antagonist protocols. In the POSEIDON group 1 population, the GnRH antagonist protocols resulted in a shorter time to live birth.

ObjectiveTo compare the clinical outcomes of dydrogesterone (DYG) and medroxyprogesterone (MPA) in the progestin-primed ovarian stimulation (PPOS) protocol for patients with poor ovarian response (POR).Patients and MethodsThis was a retrospective cohort study. Women with POR who underwent IVF/ICSI at the Reproductive Center of Third Affiliated Hospital of Zhengzhou University between January 2020 and January 2021 were included. The primary outcome measure of our study was the number of oocytes retrieved. The secondary outcome measures in the present study were the number of 2PN, number of available embryos, oocyte retrieval rate, fertilization rate, viable embryo rate per oocyte retrieved, cancellation rate and pregnancy outcomes of the first embryo transfer cycle, including the biochemical pregnancy, clinical pregnancy and miscarriage rates.ResultsIn total, 118 women underwent hMG +DYG protocols, and 692 women who underwent hMG +MPA met the Bologna criteria for POR. After baseline characteristics were balanced using the PSM model, 118 hMG +DYG protocols were matched to 118 hMG +MPA protocols, and the baseline characteristics were comparable between the two groups. The numbers of oocytes retrieved, 2PN, and available embryos and the oocyte retrieval rate, fertilization rate, viable embryo rate per oocyte retrieved and cancellation rate of the hMG+DYG and hMG+MPA protocols were comparable. Altogether, 66 women in the hMG+DYG group and 87 women in the hMG+MPA group underwent first embryo transfers. In the hMG+DYG group, 81.8% (54/66) of the patients underwent cleavage embryo transfers; similarly, 79.3% (69/87) of patients in the hMG+MPA group had cleavage embryo transfers (P=0.70).The biochemical pregnancy rate of the hMG+DYG group was 42.4%, and this was comparable to the rate in the hMG+DYG group, at 34.5% (P=0.32). The clinical pregnancy rates were similar between the two groups (36.4% vs. 31.0%, P=0.49), and there was no significant difference in the rate of miscarriage between the two groups (12.5% vs. 29.6%, P=0.14).ConclusionFor women with POR, the clinical outcome of the hMG + DYG group was similar to that of the hMG + MPA group, indicating that both combinations can be useful options for PPOS protocols.

ObjectiveTo explore whether endometrial thickness (EMT) ≤7.5 mm is associated with singleton low birth weight (LBW) from single fresh blastocyst transfer.MethodsThis was a retrospective cohort study. Only women ≤ 40 years old who underwent single fresh blastocyst transfer and had singleton live births were included in the study. LBW was the primary outcome of this study. Neonatal malformation was the secondary outcome. Logistic regression was used to evaluate the association between (EMT) ≤7.5 mm and singleton LBW.ResultsA total number of 2847 women met the study inclusion criteria. The neonatal birthweight in the EMT ≤7.5 mm group was significantly lower than that in the EMT 7.6~12.0 mm and EMT >12.0 mm group (P12.0 mm group (P12.0 mm groups. The total neonatal malformation rate was comparable between the groups.

Thermal efficiency and power density improvement are the main research foci of the literature on diesel engines. The Miller cycle is considered to be one of the most promising methods of diesel engine operation. In this study, a fully variable valve timing and lift mechanism (CD-HFVVS) was studied to determine the possibility of a Miller operation. Firstly, the valve seat impact buffer in the mechanism was tested, which proved that the buffer can effectively eliminate the valve seat impact. Then the influences of different speeds and oil temperatures were studied. The results show that the valve opening duration is prolonged when engine speed increases, and the valve lift and duration are reduced while the oil temperature increased. The valve timing and lift can be fully adjusted by changing the oil discharge position and the initial plunger position, which further proves that CD-HFVVS can achieve the performance optimization of the Miller cycle. By using the mechanism, a single cylinder test was performed. By using variable inlet valve timing, the fuel efficiency can be effectively improved and the peak pressure and in-cylinder average temperature can both be suppressed.

Spelt wheat (Triticum spelta L., 2n=6x=42, AABBDD) is a valuable source of new gene resources for wheat genetic improvement. In the present study, two novel high molecular weight glutenin subunits (HMW-GS) 1Ax2.1* at Glu-A1 and 1By19* at Glu-B1 from German spelt wheat were identified. The encoding genes of both subunits were amplified and cloned by allele-specific PCR (AS-PCR), and the complete sequences of open reading frames (ORF) were obtained. 1Ax2.1* with 2478 bp and 1By19* with 2163 bp encoded 824 and 720 amino acid residues, respectively. Molecular characterization showed that both subunits had a longer repetitive region, and high percentage of α-helices at the N- and C-termini, which are beneficial for forming superior gluten macropolymers. Protein modelling by AlphaFold2 revealed similar three-diamensional (3D) structure features of 1Ax2.1* with two x-type superior quality subunits (1Ax1 and 1Ax2*) and 1By19* with four y-type superior quality subunits (1By16, 1By9, 1By8 and 1By18). Four cysteine residues in the three x-type subunits (1Ax2.1*, 1Ax1 and 1Ax2*) and the cysteine in intermediate repeat region of y-type subunits were not expected to participate in intramolecular disulfide bond formation, but these cysteines might form intermolecular disulfide bonds with other glutenins and gliadins to enhance gluten macropolymer formation. The SNP-based molecular markers for 1Ax2.1* and 1By19* genes were developed, which were verified in different F2 populations and recombination inbred lines (RILs) derived from crossing between spelt wheat and bread wheat cultivars. This study provides data on new glutenin genes and molecular markers for wheat quality improvement.

Galfenol owing excellent deformation due to lattice softening are regarded as a new generation of smart magnetostrictive materials. However, the lack of direct probes of phase transformation and intermediate phase related to lattice softening blocks the comprehensive understanding of their intrinsic magnetostrictive mechanism and further improvement of their performance. In this work, we firstly report an atomic observation of ω phase transformation in Galfenol under low temperature aging by spherical aberration-corrected transmission electron microscopy. The ω precipitates with two variants are directly probed to be decomposed from FeGa bcc matrix with the assistances of both spinodal decomposition and displacive transformation. Their orientation relationships with the long range ordered bcc structure of D03 can be well indexed into (0003)ω1[112¯0]ω1 || (44¯4¯)D03[01¯1]D03 || (4¯401¯)ω2[112¯0]ω2. Density functional theory calculations unveil the precipitate of ω phase in Galfenol is theoretically possible. Further magnetostrictive measurements reveal the ω phase precipitates deteriorate the magnetostriction of Galfenol as empirically expected. Our work is believed to contribute a further insight of precipitate and structural evolution in Galfenol and is significant to maintain the magnetostriction performance of Galfenol in service.

Background: The efficacy of synbiotics, probiotics, prebiotics, enteral nutrition or adjuvant peripheral parenteral nutrition (EPN) and total parenteral nutrition (TPN) in preventing nosocomial infection (NI) in critically ill adults has been questioned. We conducted a systematic review and network meta-analysis (NMA) of randomized controlled trials (RCTs) to evaluate and rank the effectiveness of these therapies on NI amongst critically ill adults.Methods: Four electronic databases were systematically searched up to June 30, 2019 for RCTs comparing the administration of probiotics, prebiotics, synbiotics, EPN and TPN in critically ill adults. The primary outcome was NI. The relative efficacy of all outcomes was determined by a Bayesian framework with random effects NMA. We estimated the odds ratio (OR) and mean difference (MD) and ranked the comparative effects of all regimens with the surface under the cumulative ranking probabilities. The study has been registered on PROSPERO (CRD42019147032).Results: Fifty-five RCTs (7,119 patients) were identified. Primary outcome showed that synbiotics had the best effect in preventing NI than EPN (OR 0.37; 95% CrI 0.22–0.61), probiotics followed (OR 0.52; 95% CrI 0.34–0.77), whereas TPN significantly increased NI (OR 2.29; 95% CrI 1.48–3.67). Subgroup analysis showed that TPN significantly increased NI in intensive care unit (ICU) patients (OR 1.57; 95% CrI 1.01–2.56) and severe acute pancreatitis (SAP) patients (OR 3.93; 95% CrI 1.74–9.15). Secondary outcomes showed that synbiotics were more effective in preventing hospital-acquired pneumonia (HAP) (OR 0.34; 95% CrI 0.11–0.85), catheter-related bloodstream infection (OR 0.08; 95% CrI 0.01–0.80), urinary tract infection (OR 0.27; 95% CrI 0.08–0.71) and sepsis (OR 0.34; 95% CrI 0.16–0.70) than EPN. Amongst the treatments, probiotics were most effective for shortening the mechanical ventilation duration (MD −3.93; 95% CrI −7.98 to −0.02), prebiotics were most effective for preventing diarrhea (OR 0.24; 95% CrI 0.05–0.94) and TPN was the least effective in shortening hospital length of stay (MD 4.23; 95% CrI 0.97–7.33).Conclusions: Amongst the five therapies, synbiotics not only prevented NI in critically ill adults but also demonstrated the best treatment results. By contrast, TPN did not prevent NI and ranked last, especially in ICU and SAP patients.Take-Home Message: Nosocomial infection is a leading cause of mortality in critically ill patients in the ICU. However, the efficacy of synbiotics, probiotics, prebiotics, enteral nutrition or adjuvant peripheral parenteral nutrition and total parenteral nutrition in preventing nosocomial infection in critically ill adults has been questioned. The network meta-analysis provides evidence that amongst the five therapies, synbiotics not only prevented NI in critically ill adults but also demonstrated the best treatment results. By contrast, TPN did not prevent NI and ranked last, especially in ICU and SAP patients. The results of this study will provide a new scientific basis and a new idea for the debate on the efficacy of synbiotics and other treatments in the improvement of prognosis in critically ill adult patients.Tweet: Synbiotic prevents nosocomial infection in critically ill adults, while total parenteral nutrition has the adverse curative.

Secure outsourced aggregation in the Internet of Things (IoT) can solve the problem that sensing devices are limited in energy and bandwidth by outsourcing data aggregation task to a third-party service provider. Location-based secure outsourced aggregation (LBOA), aggregating data whose location satisfies user's location strategy, is very important in some location-critical scenarios (e.g., smart homes, intelligent transportation, and smart city). Recent work studied secure data aggregation to reduce transmission overhead and network bandwidth by optimizing topology of networks or adopting the cryptographic approach. However, as far as we know, scarcely any work considers the location information of the data source and the privacy protection of the data at the same time in the studies of secure outsourced aggregation. In this paper, we first propose an LBOA scheme LBOAMax, which can return the maximum value of sensory data whose location satisfies location strategy by applying one-way chain, order-preserving encryption, and some other cryptographic operation. Then, we proposed scheme LBOATop-k and scheme LBOASum, which can return the largest k values of data and the summation value of data based on location, respectively. The security analysis results show that our schemes can satisfy the defined requirements and the experiment results show that our schemes are feasible and efficient for each entity in practice.

BackgroundThe use of frozen embryo transfer (FET) cycles has dramatically risen. The optimal endometrial preparation method for women undergoing FET is of utmost importance to provide the optimal chances of pregnancy. For patients with abnormal ovulation in particular, there have been few studies on FET protocols; notably, most of these studies focus only on the clinical pregnancy rate or live birth rate (LBR) and pay little attention to the regimen’s safety for offspring.MethodsIt was a retrospective cohort study. First FET cycle with a single blastocyst from whole embryo frozen IVF/ICSI at the Reproductive Center of Third Affiliated Hospital of Zhengzhou University between January 2016 and January 2020. The LBR was the primary outcome of interest. The secondary outcome measures were miscarriage rate and offspring safety, including preterm birth, low birthweight (LBW), small-for-gestational age (SGA), macrosomia and large-for-gestational age (LGA).ResultsIn total, 2782 FET cycles met the eligibility criteria for analysis. Additionally, there were 1178 singleton births from FET cycles. The clinical pregnancy rate was 58.4% in the L-FET group and 54.5% in the HRT group, with no statistical significance (P=.116). The miscarriage rate was higher in the HRT group than in the L-FET group (21.7% vs. 14.3%, P=.005). The LBR was significantly higher in the L-FET group than in the HRT group (49.6% vs. 41.7%, P=.001). Neonatal outcomes were similar between the two groups. After adjustments for confounding factors, the LBR was higher in the L-FET group (aOR 1.30, 95% CI 1.06-1.58). The rate of miscarriage was lower in the L-FET group (aOR 0.63, 95% CI 0.44-0.90).ConclusionFor patients with abnormal ovulation, the L-FET regimen has a higher LBR and lower miscarriage rate than HRT. The neonatal outcomes were similar between the two groups.

Previous studies have established that disturbed lymphocytes are involved in the pathogenesis of Vogt-Koyanagi-Harada (VKH) syndrome. Accordingly, glucocorticoids (GCs), with their well-recognized immune-suppressive function, have been widely used for treatment of VKH patients with acute relapses. However, the systemic response of diverse immune cells to GC therapy in VKH is poorly characterized. To address this issue, we analyzed immune cell subpopulations and their phenotype, as well as cytokine profiles in peripheral blood from VKH patients (n=25) and health controls (HCs, n=21) by flow cytometry and luminex technique, respectively. For 16 patients underwent GC therapy (methylprednisolone, MP), the aforementioned measurements as well as the transcriptome data from patients before and after one-week’s GC therapy were also compared to interrogate the systemic immune response to GC therapy. Lymphocyte composition in the blood was different in VKH patients and HCs. VKH patients had significantly higher numbers of T cells with more activated, polarized and differentiated phenotype, more unswitched memory B cells and monocytes, as compared to HCs. MP treatment resulted in decreased frequencies of T cells and NK cells, inhibited NK cell activation and T cell differentiation, and more profoundly, a marked shift in the distribution of monocyte subsets. Collectively, our findings suggest that advanced activation and differentiation, as well as dysregulated numbers of peripheral lymphocytes are the major immunological features of VKH, and GC therapy with MP not only inhibits T cell activation directly, but also affects monocyte subsets, which might combinatorically result in the inhibition of the pathogenic immune response.

The Multidrug and toxin efflux (MATE) gene family plays crucial roles in plant growth and development and response to adverse stresses. This work investigated the structural and evolutionary characteristics, expression profiling and potential functions involved in aluminium (Al) tolerance from a genome-wide level. In total, 211 wheat MATE genes were identified, which were classified into four subfamilies and unevenly distributed on chromosomes. Duplication analysis showed that fragments and tandem repeats played the main roles in the amplification of TaMATEs, and Type II functional disproportionation had a leading role in the differentiation of TaMATEs. TaMATEs had abundant Al resistance and environmental stress-related elements, and generally had a high expression level in roots and leaves and in response to Al stress. The 3D structure prediction by AlphaFold and molecular docking showed that six TaMATE proteins localised in the plasmalemma could combine with citrate via amino acids in the citrate exuding motif and other sites, and then transport citrate to soil to form citrate aluminium. Meanwhile, citrate aluminium formed in root cells might be transported to leaves by TaMATEs to deposit in vacuoles, thereby alleviating Al toxicity.

Determining the exact occupation sites of the doping ions in spinel ferrites is vital for tailoring and improving their magnetic properties. In this study, the distribution and occupation sites of cations in MgFe2O4 and Zn-doped MgFe2O4 ferrite are imaged by Cs-STEM. The experimental STEM images along [001], [011] and [111] orientations suggest that the divalent Mg2+ cations occupy all A sites, and the trivalent Fe3+ cations occupy all B sites in MgFe2O4 ferrite prepared by electrospinning, which is consistent with the normal spinel structure. We further clarify that the preferred sites of dopant Zn2+ ions are Fe3+ crystallographic sites in the Zn-doped MgFe2O4 ferrite nanofibers. Magnetic measurements show that Zn doping affects the spin states of the Fe3+, and the Fe3+-O2−-Fe3+ super-exchange interaction leads to enhancements in the magnetization and reduction in the Curie temperature. Our work should contribute a significant step toward eventually realizing the practical application of doped spinel ferrites.